Enzymes of the oxidoreductase class that catalyze the dehydrogenation of hydroxysteroids. (From Enzyme Nomenclature, 1992) EC 1.1.-.
Catalyze the oxidation of 3-hydroxysteroids to 3-ketosteroids.
A class of enzymes that catalyzes the oxidation of 17-hydroxysteroids to 17-ketosteroids. EC 1.1.-.
A group of enzymes that catalyze the reversible reduction-oxidation reaction of 20-hydroxysteroids, such as from a 20-ketosteroid to a 20-alpha-hydroxysteroid (EC 1.1.1.149) or to a 20-beta-hydroxysteroid (EC 1.1.1.53).
A 3-hydroxysteroid dehydrogenase which catalyzes the reversible reduction of the active androgen, DIHYDROTESTOSTERONE to 5 ALPHA-ANDROSTANE-3 ALPHA,17 BETA-DIOL. It also has activity towards other 3-alpha-hydroxysteroids and on 9-, 11- and 15- hydroxyprostaglandins. The enzyme is B-specific in reference to the orientation of reduced NAD or NADPH.
An high-affinity, NAD-dependent 11-beta-hydroxysteroid dehydrogenase that acts unidirectionally to catalyze the dehydrogenation of CORTISOL to CORTISONE. It is found predominantly in mineralocorticoid target tissues such as the KIDNEY; COLON; SWEAT GLANDS; and the PLACENTA. Absence of the enzyme leads to a fatal form of childhood hypertension termed, APPARENT MINERALOCORTICOID EXCESS SYNDROME.
A low-affinity 11 beta-hydroxysteroid dehydrogenase found in a variety of tissues, most notably in LIVER; LUNG; ADIPOSE TISSUE; vascular tissue; OVARY; and the CENTRAL NERVOUS SYSTEM. The enzyme acts reversibly and can use either NAD or NADP as cofactors.
Hydroxysteroid dehydrogenases that catalyzes the reversible conversion of CORTISOL to the inactive metabolite CORTISONE. Enzymes in this class can utilize either NAD or NADP as cofactors.
Enzymes that catalyze the oxidation of estradiol at the 17-hydroxyl group in the presence of NAD+ or NADP+ to yield estrone and NADH or NADPH. The 17-hydroxyl group can be in the alpha- or beta-configuration. EC 1.1.1.62
Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.
A naturally occurring glucocorticoid. It has been used in replacement therapy for adrenal insufficiency and as an anti-inflammatory agent. Cortisone itself is inactive. It is converted in the liver to the active metabolite HYDROCORTISONE. (From Martindale, The Extra Pharmacopoeia, 30th ed, p726)
A microsomal cytochrome P450 enzyme that catalyzes the 17-alpha-hydroxylation of progesterone or pregnenolone and subsequent cleavage of the residual two carbons at C17 in the presence of molecular oxygen and NADPH-FERRIHEMOPROTEIN REDUCTASE. This enzyme, encoded by CYP17 gene, generates precursors for glucocorticoid, androgen, and estrogen synthesis. Defects in CYP17 gene cause congenital adrenal hyperplasia (ADRENAL HYPERPLASIA, CONGENITAL) and abnormal sexual differentiation.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed)
A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)
The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.
The rate dynamics in chemical or physical systems.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A metabolite of TESTOSTERONE or ANDROSTENEDIONE with a 3-alpha-hydroxyl group and without the double bond. The 3-beta hydroxyl isomer is epiandrosterone.
A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen.
Enzymes that catalyze the dehydrogenation of GLYCERALDEHYDE 3-PHOSPHATE. Several types of glyceraldehyde-3-phosphate-dehydrogenase exist including phosphorylating and non-phosphorylating varieties and ones that transfer hydrogen to NADP and ones that transfer hydrogen to NAD.
An enzymes that catalyzes the reversible reduction-oxidation reaction of 20-alpha-hydroxysteroids, such as from PROGESTERONE to 20-ALPHA-DIHYDROPROGESTERONE.
An enzyme that oxidizes an aldehyde in the presence of NAD+ and water to an acid and NADH. This enzyme was formerly classified as EC 1.1.1.70.
An enzyme that catalyzes the conversion of L-glutamate and water to 2-oxoglutarate and NH3 in the presence of NAD+. (From Enzyme Nomenclature, 1992) EC 1.4.1.2.
An enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. EC 1.1.1.37.
An enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. (From Dorland, 27th ed) (The NADP+ enzyme is EC 1.1.1.42.) EC 1.1.1.41.
3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.
A sulfotransferase that catalyzes the sulfation of a phenol in the presence of 3'-phosphoadenylylsulfate as sulfate donor to yield an aryl sulfate and adenosine 3',5'-bisphosphate. A number of aromatic compounds can act as acceptors; however, organic hydroxylamines are not substrates. Sulfate conjugation by this enzyme is a major pathway for the biotransformation of phenolic and catechol drugs as well as neurotransmitters. EC 2.8.2.1.
Steroid derivatives formed by oxidation of a methyl group on the side chain or a methylene group in the ring skeleton to form a ketone.
Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)
A flavoprotein containing oxidoreductase that catalyzes the reduction of lipoamide by NADH to yield dihydrolipoamide and NAD+. The enzyme is a component of several MULTIENZYME COMPLEXES.
Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.
A flavoprotein containing oxidoreductase that catalyzes the dehydrogenation of SUCCINATE to fumarate. In most eukaryotic organisms this enzyme is a component of mitochondrial electron transport complex II.
An alcohol oxidoreductase which catalyzes the oxidation of L-iditol to L-sorbose in the presence of NAD. It also acts on D-glucitol to form D-fructose. It also acts on other closely related sugar alcohols to form the corresponding sugar. EC 1.1.1.14
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A major C19 steroid produced by the ADRENAL CORTEX. It is also produced in small quantities in the TESTIS and the OVARY. Dehydroepiandrosterone (DHEA) can be converted to TESTOSTERONE; ANDROSTENEDIONE; ESTRADIOL; and ESTRONE. Most of DHEA is sulfated (DEHYDROEPIANDROSTERONE SULFATE) before secretion.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A glucose dehydrogenase that catalyzes the oxidation of beta-D-glucose to form D-glucono-1,5-lactone, using NAD as well as NADP as a coenzyme.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Oxidoreductases that are specific for ALDEHYDES.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
D-Glucose:1-oxidoreductases. Catalyzes the oxidation of D-glucose to D-glucono-gamma-lactone and reduced acceptor. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.47; EC 1.1.1.118; EC 1.1.1.119 and EC 1.1.99.10.
An enzyme of the oxidoreductase class that catalyzes the reaction 6-phospho-D-gluconate and NADP+ to yield D-ribulose 5-phosphate, carbon dioxide, and NADPH. The reaction is a step in the pentose phosphate pathway of glucose metabolism. (From Dorland, 27th ed) EC 1.1.1.43.
Reversibly catalyzes the oxidation of a hydroxyl group of sugar alcohols to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2. and EC 1.1.99.
Enzymes that catalyze the first step in the beta-oxidation of FATTY ACIDS.
A flavoprotein and iron sulfur-containing oxidoreductase that catalyzes the oxidation of NADH to NAD. In eukaryotes the enzyme can be found as a component of mitochondrial electron transport complex I. Under experimental conditions the enzyme can use CYTOCHROME C GROUP as the reducing cofactor. The enzyme was formerly listed as EC 1.6.2.1.
An enzyme that catalyzes the dehydrogenation of inosine 5'-phosphate to xanthosine 5'-phosphate in the presence of NAD. EC 1.1.1.205.
Alcohol oxidoreductases with substrate specificity for LACTIC ACID.
A flavoprotein oxidoreductase that has specificity for medium-chain fatty acids. It forms a complex with ELECTRON TRANSFERRING FLAVOPROTEINS and conveys reducing equivalents to UBIQUINONE.
Flavoproteins that catalyze reversibly the reduction of carbon dioxide to formate. Many compounds can act as acceptors, but the only physiologically active acceptor is NAD. The enzymes are active in the fermentation of sugars and other compounds to carbon dioxide and are the key enzymes in obtaining energy when bacteria are grown on formate as the main carbon source. They have been purified from bovine blood. EC 1.2.1.2.
An enzyme that catalyzes the oxidation of XANTHINE in the presence of NAD+ to form URIC ACID and NADH. It acts also on a variety of other purines and aldehydes.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
A ketone oxidoreductase that catalyzes the overall conversion of alpha-keto acids to ACYL-CoA and CO2. The enzyme requires THIAMINE DIPHOSPHATE as a cofactor. Defects in genes that code for subunits of the enzyme are a cause of MAPLE SYRUP URINE DISEASE. The enzyme was formerly classified as EC 1.2.4.3.
Proteins prepared by recombinant DNA technology.
Enzymes that reversibly catalyze the oxidation of a 3-hydroxyacyl CoA to 3-ketoacyl CoA in the presence of NAD. They are key enzymes in the oxidation of fatty acids and in mitochondrial fatty acid synthesis.
The E1 component of the multienzyme PYRUVATE DEHYDROGENASE COMPLEX. It is composed of 2 alpha subunits (pyruvate dehydrogenase E1 alpha subunit) and 2 beta subunits (pyruvate dehydrogenase E1 beta subunit).
Oxidoreductases that are specific for KETONES.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
Inorganic salts of sulfuric acid.
An oxidoreductase involved in pyrimidine base degradation. It catalyzes the catabolism of THYMINE; URACIL and the chemotherapeutic drug, 5-FLUOROURACIL.
An enzyme that catalyzes the oxidation of UDPglucose to UDPglucuronate in the presence of NAD+. EC 1.1.1.22.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A disease-producing enzyme deficiency subject to many variants, some of which cause a deficiency of GLUCOSE-6-PHOSPHATE DEHYDROGENASE activity in erythrocytes, leading to hemolytic anemia.
A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).
One of the two major pharmacological subdivisions of adrenergic receptors that were originally defined by the relative potencies of various adrenergic compounds. The alpha receptors were initially described as excitatory receptors that post-junctionally stimulate SMOOTH MUSCLE contraction. However, further analysis has revealed a more complex picture involving several alpha receptor subtypes and their involvement in feedback regulation.

26-cholesterol hydroxylase in rat corpora lutea: A negative regulator of progesterone secretion. (1/46)

From a subtracted cDNA library of rat luteal tissue, where cDNA fragments in functional luteal tissue were subtracted from those in regressing luteal tissue, a cDNA clone corresponding to 26-cholesterol hydroxylase (P450(C26)) was obtained. It is known that P450(C26) catalyzes the conversion of cholesterol to 26-hydroxycholesterol, which blocks cholesterol utilization in the cell, and that 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) catalyzes the conversion of progesterone to an inactive steroid, 20alpha-dihydroprogesterone (20alpha-OHP). Thus, using pseudopregnant rats as a model, physiological cooperation of P450(C26) and 20alpha-HSD in the reduction of progesterone release toward the end of the luteal phase was evaluated. Levels of P450(C26) and 20alpha-HSD mRNA were examined in corpora lutea from pseudopregnant rats by Northern blot or reverse transcription-polymerase chain reaction or both. P450(C26) mRNA was ubiquitously expressed in corpora lutea, and its expression increased toward the end of pseudopregnancy, while 20alpha-HSD was expressed in all corpora lutea on Day 16 (Day 0 = the day of after cervical stimulation) but not detected before Day 10. An inhibitor of 20alpha-HSD, STZ26 (D-homo-16-oxa-4-androstene-3,16alpha-dione), was administered at various doses to rats from Day 12 to 20, effectively suppressing the elevation of 20alpha-OHP in a dose-dependent manner but not the depletion of progesterone completely. The expression of P450(C26) mRNA was increased as STZ26 dose increased, which negatively correlated with the progesterone levels. These results strongly suggest that P450(C26) cooperated with 20alpha-HSD in the reduction of progesterone release from the rat luteal tissue at the end of the functional luteal phase.  (+info)

Conversion of mammalian 3alpha-hydroxysteroid dehydrogenase to 20alpha-hydroxysteroid dehydrogenase using loop chimeras: changing specificity from androgens to progestins. (2/46)

Hydroxysteroid dehydrogenases (HSDs) regulate the occupancy and activation of steroid hormone receptors by converting potent steroid hormones into their cognate inactive metabolites. 3alpha-HSD catalyzes the inactivation of androgens in the prostate by converting 5alpha-dihydrotestosterone to 3alpha-androstanediol, where excess 5alpha-dihydrotestosterone is implicated in prostate disease. By contrast, 20alpha-HSD catalyzes the inactivation of progestins in the ovary and placenta by converting progesterone to 20alpha-hydroxyprogesterone, where progesterone is essential for maintaining pregnancy. Mammalian 3alpha-HSDs and 20alpha-HSDs belong to the aldo-keto reductase superfamily and share 67% amino acid sequence identity yet show positional and stereospecificity for the formation of secondary alcohols on opposite ends of steroid hormone substrates. The crystal structure of 3alpha-HSD indicates that the mature steroid binding pocket consists of 10 residues located on five loops, including loop A and the mobile loops B and C. 3alpha-HSD was converted to 20alpha-HSD by replacing these loops with those found in 20alpha-HSD. However, when pocket residues in 3alpha-HSD were mutated to those found in 20alpha-HSD altered specificity was not achieved. Replacement of loop A created a 17beta-HSD activity that was absent in either 3alpha- or 20alpha-HSD. Once loops A and C were replaced, the chimera had both 3alpha- and 20alpha-HSD activity. When loops A, B, and C were substituted, 3alpha-HSD was converted to a stereospecific 20alpha-HSD with a resultant shift in k(cat)/K(m) for the desired reaction of 2 x 10(11). This study represents an example where sex hormone specificity can be changed at the enzyme level.  (+info)

Prostaglandin F2alpha-induced expression of 20alpha-hydroxysteroid dehydrogenase involves the transcription factor NUR77. (3/46)

Prostaglandin F(2)alpha (PGF(2)alpha) binding to its receptor on the rat corpus luteum triggers various signal transduction pathways that lead to the activation of a steroidogenic enzyme, 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD), which in turn catabolizes progesterone. The molecular mechanism underlying PGF(2)alpha-induced 20alpha-HSD enzyme activity has not yet been explored. In this report we show, using mice lacking PGF(2)alpha receptor and pregnant rats, that PGF(2)alpha is responsible for the rapid and massive expression of the 20alpha-HSD gene at the end of pregnancy leading to a decrease in progesterone secretion. We also present evidence that PGF(2)alpha enhances 20alpha-HSD promoter activity. We have determined a region upstream of the -1590 position in the 20alpha-HSD promoter that confers regulation by PGF(2)alpha in ovarian primary cells. This region encompasses a unique transcription factor-binding site with a sequence of a NUR77 response element. Deletion of this motif or overexpression of a NUR77 dominant negative protein caused a complete loss of 20alpha-HSD promoter activation by PGF(2)alpha. NUR77 also transactivated the 20alpha-HSD promoter in transient transfection experiments in corpus luteum-derived cells (GG-CL). This induction required the NUR77-transactivating domain. We also show that PGF(2)alpha induces a very rapid expression of NUR77 that binds to a distal response element located at -1599/-1606 but does not interact with another proximal putative NUR77 response element located downstream in the promoter. A rapid increase in NUR77 mRNA was observed in mice corpora lutea just before parturition at a time when 20alpha-HSD becomes expressed. This increase in the expression of both genes was not seen in PGF(2)alpha receptor knockout mice. By using cyclosporin A and PGF(2)alpha treatment, we established that inhibition of NUR77 DNA binding in vivo prevents PGF(2)alpha induction of the 20alpha-HSD gene in the corpus luteum. Taken together, our results demonstrate, for the first time, that PGF(2)alpha induces in the corpus luteum the expression of the nuclear orphan receptor and transcription factor, NUR77, which in turn leads to the transcriptional stimulation of 20alpha-HSD, triggering the decrease in serum progesterone essential for parturition.  (+info)

Characterization of a human 20alpha-hydroxysteroid dehydrogenase. (4/46)

It has been suggested that 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) is a T-cell differentiation marker in mice. In the human, this enzyme has generally been associated with types 1 and 2 17beta-HSDs, which belong to the short-chain alcohol dehydrogenase family, whereas the rat, rabbit, pig and bovine 20alpha-HSDs are members of the aldoketo reductase superfamily, which also includes the 3alpha-HSD family. In this study, we report the cloning, from a human skin cDNA library, of a cDNA that shows, after transfection into human embryonic kidney (HEK-293) cells, high 20alpha-HSD activity but negligible 3alpha- and 17beta-hydroxysteroid dehydrogenase activities. A comparison of the amino acid sequence of the human 20alpha-HSD with those of other related 20alpha- and 3alpha-HSDs indicates that the human 20alpha-HSD shares 79.9, 68.7 and 52.3% identity with rabbit, rat and bovine 20alpha-HSDs, whereas it shows 97, 84 and 65% identity with human type 3, type 1 and rat 3alpha-HSDs. In contrast, the enzyme shares only 15.2 and 15.0% identity with type 1 and type 2 human 17beta-HSDs. DNA analysis predicts a protein of 323 amino acids, with a calculated molecular weight of 36 767 Da. In intact transfected cells, the human 20alpha-HSD preferentially catalyzes the reduction of progesterone to 20alpha-hydroxyprogesterone with a K(m) value of 0.6 microM, the reverse reaction (oxidation) being negligible. In a cell cytosolic preparation, the enzyme could use both NADPH and NADH as cofactors, but NADPH, which gave 4-fold lower K(m) values, was preferred. We detected the expression of 20alpha-HSD mRNA in liver, prostate, testis, adrenal, brain, uterus and mammary-gland tissues and in human keratinocyte (HaCaT) cells. The present study clearly indicates that the genuine human 20alpha-HSD belongs to the aldoketo reductase family, like the 20alpha-HSDs from other species.  (+info)

Dependence on prolactin of the luteolytic effect of prostaglandin F2alpha in rat luteal cell cultures. (5/46)

Luteal regression is a multistep, prolonged process, and long-term luteal cultures are required for studying it in vitro. Cell suspensions from ovaries of superovulated rats were enriched with steroidogenic cells, seeded on laminin or fibronectin, and maintained in defined medium for up to 10 days. Progesterone secretion was much lower than that of 20alpha-dihydroprogesterone, a product of 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD). Prolactin added throughout the incubation period gradually increased the percent progesterone out of total progestins to fourfold, while reducing 20alpha-HSD mRNA by 73%. Luteinizing hormone accelerated the establishment of higher percent progesterone by prolactin but by itself had no effect. Prolactin did not increase total progestin production or cytochrome P450 side-chain cleavage (P450(scc)) mRNA. Cell viability was unaffected by prolactin and/or LH. Prostaglandin F2alpha (PGF2alpha) was added 7-8 days after seeding. In prolactin-treated cells, PGF2alpha reduced steroidogenesis after 4-45 h, and at 45 h total progestins and P450(scc) mRNA were reduced by 45%. At 8-45 h PGF2alpha reduced the percent progesterone out of total progestins, and at 45 h 20alpha-HSD mRNA was doubled. In contrast, in prolactin-deprived cultures, PGF2alpha had little effect on total progestins or 20alpha-HSD mRNA but doubled P450(scc) mRNA. Phospholipase C activity was stimulated by PGF2alpha regardless of prolactin. Thus, when prolactin-treated, our cultures are a good model for mature corpora lutea challenged with PGF2alpha; the finding that without prolactin PGF2alpha has an alternative set of actions could help in identifying the signaling pathways of PGF2alpha responsible for its luteolytic effects.  (+info)

Luteal expression of cytochrome P450 side-chain cleavage, steroidogenic acute regulatory protein, 3beta-hydroxysteroid dehydrogenase, and 20alpha-hydroxysteroid dehydrogenase genes in late pregnant rats: effect of luteinizing hormone and RU486. (6/46)

A decrease in serum progesterone at the end of pregnancy is essential for the induction of parturition in rats. We have previously demonstrated that LH participates in this process through: 1) inhibiting 3beta-hydroxysteroid dehydrogenase (3beta-HSD) activity and 2) stimulating progesterone catabolism by inducing 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) activity. The objective of this investigation was to determine the effect of LH and progesterone on the luteal expression of the steroidogenic acute regulatory protein (StAR), cytochrome P450 side-chain cleavage (P450(scc)), 3beta-HSD, and 20alpha-HSD genes. Gene expression was analyzed by Northern blot analysis 24 and 48 h after administration of LH or vehicle on Day 19 of pregnancy. StAR and 3beta-HSD mRNA levels were lower in LH-treated rats than in rats administered with vehicle at both time points studied. P450(scc) mRNA levels were unaffected by LH. The 20alpha-HSD mRNA levels were not different between LH and control rats 24 h after treatment; however, greater expression of 20alpha-HSD, with respect to controls, was observed in LH-treated rats 48 h after treatment. Luteal progesterone content dropped in LH-treated rats at both time points studied, whereas serum progesterone decreased after 48 h only. In a second set of experiments, the anti-progesterone RU486 was injected intrabursally on Day 20 of pregnancy. RU486 had no effect on 3beta-HSD or P450(scc) expression but increased 20alpha-HSD mRNA levels after 8 h treatment. In conclusion, the luteolytic effect of LH is mediated by a drop in StAR and 3beta-HSD expression without effect on P450(scc) expression. We also provide the first in vivo evidence indicating that a decrease in luteal progesterone content may be an essential step toward the induction of 20alpha-HSD expression at the end of pregnancy in rats.  (+info)

Progesterone metabolism in human leukemic monoblast U937 cells. (7/46)

Progesterone markedly inhibits the functions of human macrophages and T lymphocytes, and acts as an immunosuppressant during pregnancy. It is important to examine progesterone metabolites to understand the overall bioactive properties of this sex steroid. However, progesterone metabolism has not been examined in human immune cells. The human leukemic monoblast U937 cell line exhibits monocytic lineage and provides a valuable model to analyze monocyte-macrophage differentiation. Therefore, in this study, we analyzed progesterone metabolism in U937 cells by thin-layer chromatography. Progesterone was metabolized to 5alpha-pregnan-3beta,6alpha-diol-20-one via 5alpha-dihydroprogesterone and 5alpha-pregnan-3beta-ol-20-one, and 5alpha-pregnan-3beta,20alpha-diol was also detected as a final metabolic product via 20alpha-dihydroprogesterone and 5alpha-pregnan-20alpha-ol-3-one. 5alpha-reduction (5alpha-reductase type 1) and 20alpha-reduction were involved in the first step of metabolism. To identify the enzyme responsible for the 20alpha-reduction, we screened an U937 cDNA library, and obtained a clone (1.2 kb), which was identical to the human hepatic bile acid-binding protein or 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD). 293 cells transfected with this cDNA demonstrated marked 20alpha-reduction of progesterone to 20alphaDHP, but 20alpha-oxidative, 3alpha-HSD or 17beta-HSD activity was found to be negligible. In experimental animals, the importance of 20alpha-HSD has been reported to be involved in the protection of immune cells from the toxic effects of progesterone. Therefore, our present data suggest that 20alpha-HSD plays an important role in the regulation of progesterone actions in human immune cells.  (+info)

Effects of deletion of the prolactin receptor on ovarian gene expression. (8/46)

Prolactin (PRL) exerts pleiotropic physiological effects in various cells and tissues, and is mainly considered as a regulator of reproduction and cell growth. Null mutation of the PRL receptor (R) gene leads to female sterility due to a complete failure of embryo implantation. Pre-implantatory egg development, implantation and decidualization in the mouse appear to be dependent on ovarian rather than uterine PRLR expression, since progesterone replacement permits the rescue of normal implantation and early pregnancy. To better understand PRL receptor deficiency, we analyzed in detail ovarian and corpora lutea development of PRLR-/- females. The present study demonstrates that the ovulation rate is not different between PRLR+/+ and PRLR-/- mice. The corpus luteum is formed but an elevated level of apoptosis and extensive inhibition of angiogenesis occur during the luteal transition in the absence of prolactin signaling. These modifications lead to the decrease of LH receptor expression and consequently to a loss of the enzymatic cascades necessary to produce adequate levels of progesterone which are required for the maintenance of pregnancy.  (+info)

Weinstein, Y, 20alpha-hydroxysteroid Dehydrogenase. A t lymphocyte-associated enzyme. (1977). Subject Strain Bibliography 1977. 930 ...
1J96: Structure of the human 3alpha-hydroxysteroid dehydrogenase type 3 in complex with testosterone and NADP at 1.25-A resolution.
1MRQ: Human 20alpha-hydroxysteroid dehydrogenase: crystallographic and site-directed mutagenesis studies lead to the identification of an alternative binding site for C21-steroids.
3cv7: Structure of aldehyde reductase in ternary complex with coenzyme and the potent 20alpha-hydroxysteroid dehydrogenase inhibitor 3,5-dichlorosalicylic acid: implications for inhibitor binding and selectivity.
Also acts on other 17beta-hydroxysteroids and on the 3alpha-hydroxy group of pregnanes and bile acids. Different from EC 1.1.1.50 3alpha-hydroxysteroid dehydrogenase (Si-specific) or EC 1.1.1.213 3alpha-hydroxysteroid dehydrogenase (Re-specific ...
trans-1,2-dihydrobenzene-1,2-diol dehydrogenase: rat liver cytosol enzyme also catalyzes 3alpha-hydroxysteroid dehydrogenase activity (EC 1.1.1.50); GenBank AH009074 (rat); RefSeq NM_001818 (human)
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011 ...
From NCBI Gene:. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]. From UniProt: ...
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Dab (Limanda limanda) ovarian fragments were incubated in vitro with either [4,7-3H]pregnenolone or 17 alpha-hydroxy[1,2,6,7-3H]progesterone to investigate the pattern of steroidogenesis. A major enzyme found in the dab ovary was 20 alpha-hydroxysteroid dehydrogenase. Among the steroids that were tentatively identified in ovarian incubates were 17 alpha,20 alpha-dihydroxy-4-pregnen-3-one (17,20 alpha-P). 17 alpha,20 alpha-dihydroxy-5 beta-pregnan-3-one, 3 beta, 17 alpha,20 alpha-trihydroxy-5 beta-pregnane (3 beta,17,20 alpha-P-5 beta), and 3 alpha,17 alpha,20 alpha-trihydroxy-5 beta-pregnane. The presence of these steroids in plasma of mature female and male dabs was studied by radioimmunoassay. The antiserum was raised against 17,20 alpha-P. The 17,20 alpha-[3H]P label was produced by incubating place milt with 17 alpha-hydroxy [3H]progesterone. The radioimmunoassay was shown to have a high cross-reaction with the 5 beta-reduced analogues of 17,20 alpha-P and was therefore used, in conjunction ...
11beta,21-Dihydroxy-3,20-oxo-5beta-pregnan-18-al is an intermediate in C21-Steroid hormone metabolism. 11beta,21-Dihydroxy-3,20-oxo-5beta-pregnan-18-al is converted from Aldosterone via the enzyme 3-oxo-5beta-steroid 4-dehydrogenase (EC:1.3.99.6). It is then converted to 3alpha,11beta,21-Trihydroxy-20-oxo-5beta-pregnan-18-al via the enzyme 3-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.50 ...
Rat Luteal Cell Cultures Seeded and Incubated in the Absence of Serum: Long- and Short-Term Effects of Hormones, and Influence of the Extracellular ...
Expression of DHRS9 (3alpha-HSD, RDH15, RDHL, RETSDR8, SDR9C4) in skeletal muscle tissue. Antibody staining with HPA036491 and HPA036667 in immunohistochemistry.
Antibody information for antibodies HPA036491 and HPA036667 used in analysis of ENSG00000073737 / DHRS9 (3alpha-HSD, RDH15, RDHL, RETSDR8, SDR9C4)
kód: 0653 rod: Dryopteris druh: Dryopteris erythrosora Prolifica čeľaď: Polypodiaceae syn: Dryopteris erythrosora var. prolifica slov. názov:
Evidence is reported for the existence of a structurally and functionally related and probably evolutionarily conserved class of membrane-bound liver carbonyl reductases/hydroxysteroid dehydrogenases involved in steroid and xenobiotic carbonyl metabolism. Carbonyl reduction was investigated in liver microsomes of 8 vertebrate species, as well as in insect larvae total homogenate and in purified 3 alpha-hydroxysteroid dehydrogenase preparations of the procaryont Pseudomonas testosteroni, using the ketone compound 2-methyl-1,2 di-(3-pyridyl)-1-propanone (metyrapone) as substrate. The enzyme activities involved in the metyrapone metabolism were screened for their sensitivity to several steroids as inhibitors. In all fractions tested, steroids of the adrostane or pregnane class strongly inhibited xenobiotic carbonyl reduction, whereas only in the insect and procaryotic species could ecdysteroids inhibit this reaction. Immunoblot analysis with antibodies against the respective microsomal mouse liver
Hydroxysteroid Dehydrogenase (3.BETA.-HSD), 17.ALPHA.-Hydroxylase and 17, 20 Lyase by Progestins and Danazol". Endocrinologia ... p. 5. Archived from the original (RTF) on 2006-06-20. Retrieved 2006-06-01. Arakawa, Satoko; Mitsuma, Mizue; Iyo, Masato; ... 11-trien-20-yn-17β-ol-3-one, is a synthetic estrane steroid and a derivative of testosterone. It is more specifically a ...
3-alpha)-hydroxysteroid dehydrogenase)". National Center for Biotechnology Information, U.S. National Library of Medicine. CS1 ... 3α-hydroxysteroid dehydrogenase type 3, and dihydrodiol dehydrogenase type 2, is an enzyme that in humans is encoded by the ... This enzyme binds bile acid with high affinity, and shows minimal 3α-hydroxysteroid dehydrogenase activity. This gene shares ... "Entrez Gene: AKR1C2 aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 1; 20-alpha ( ...
RAG1 Alpha-2-plasmin inhibitor deficiency; 262850; PLI Alpha-ketoglutarate dehydrogenase deficiency; 203740; OGDH Alpha- ... CYP17A1 17-beta-hydroxysteroid dehydrogenase X deficiency; 300438; HSD17B10 2-methylbutyrylglycinuria; 610006; ACADSB 3- ... PC Pyruvate dehydrogenase deficiency; 312170; PDHA1 Pyruvate dehydrogenase E2 deficiency; 245348; DLAT Pyruvate dehydrogenase ... SCARB2 Acyl-CoA dehydrogenase, long chain, deficiency of; 201460; ACADL Acyl-CoA dehydrogenase, medium chain, deficiency of; ...
Khanna M, Qin KN, Cheng KC (Jun 1995). "Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular cloning ... "Close kinship of human 20alpha-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes". Genes to Cells. 5 (2): ... Bennett MJ, Schlegel BP, Jez JM, Penning TM, Lewis M (Aug 1996). "Structure of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase ... "Entrez Gene: AKR1C3 aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)". Theisen, J. ...
Hepatic metabolism is mediated by 11 beta-hydroxysteroid dehydrogenases (11[beta]-HSD) and 20-ketosteroid reductases. ... alpha]-hydroxymethylprednisolone. As stated previously, the primary use of methylprednisolone is to suppress inflammatory and ... 20 mg/day of prednisone (16 mg/day of methylprednisolone) is the threshold dosage for PAE development agreed upon by many ... Depo-Medrol is available as sterile aqueous solution in 20 mg/mL, 40 mg/mL, or 80 mg/mL strengths. Solu-Medrol is the only ...
... alpha-hydroxysteroid dehydrogenase for neurosteroids and its inhibition by benzodiazepines" (pdf). Biological & Pharmaceutical ... while 3β-hydroxysteroid dehydrogenase and hydroxysteroid sulfotransferases are involved in excitatory neurosteroid production. ... 5α-reductase type I and 3α-hydroxysteroid dehydrogenase are involved in the biosynthesis of inhibitory neurosteroids, ... Melcangi RC, Celotti F, Martini L (March 1994). "Progesterone 5-alpha-reduction in neuronal and in different types of glial ...
Khanna M, Qin KN, Cheng KC (Jun 1995). "Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular cloning ... Couture JF, Legrand P, Cantin L, Luu-The V, Labrie F, Breton R (Aug 2003). "Human 20alpha-hydroxysteroid dehydrogenase: ... Zhang Y, Dufort I, Rheault P, Luu-The V (Oct 2000). "Characterization of a human 20alpha-hydroxysteroid dehydrogenase". Journal ... 3-alpha)-hydroxysteroid dehydrogenase)". Human AKR1C1 genome location and AKR1C1 gene details page in the UCSC Genome Browser. ...
... alpha-hydroxysteroid dehydrogenase for neurosteroids and its inhibition by benzodiazepines" (pdf). Biol Pharm Bull. 25 (4): 441 ... Usami N; Yamamoto T; Shintani S; Ishikura S; Higaki Y; Katagiri Y; Hara A. (Apr 2002). "Substrate specificity of human 3(20) ...
... cytokine was originally discovered via the observation that it induced the synthesis of 20alpha-hydroxysteroid dehydrogenase in ... induces disulfide-linked IL-3 receptor alpha- and beta-chain heterodimerization, which is required for receptor activation but ... induces disulfide-linked IL-3 receptor alpha- and beta-chain heterodimerization, which is required for receptor activation but ... Ihle JN, Pepersack L, Rebar L (June 1981). "Regulation of T cell differentiation: in vitro induction of 20 alpha-hydroxysteroid ...
... variations in levels of 17 beta-hydroxysteroid dehydrogenase type 2 and 5 alpha-reductase activity compatible with androgen ... 17β-Hydroxysteroid dehydrogenase 2 (17β-HSD2) is an enzyme of the 17β-hydroxysteroid dehydrogenase (17β-HSD) family that in ... dehydrogenase 2". Moeller G, Adamski J (2006). "Multifunctionality of human 17beta-hydroxysteroid dehydrogenases". Mol. Cell. ... Soubhye J, Alard IC, van Antwerpen P, Dufrasne F (2015). "Type 2 17-β hydroxysteroid dehydrogenase as a novel target for the ...
Souness GW, Morris DJ (March 1996). "11 alpha- and 11 beta-hydroxyprogesterone, potent inhibitors of 11 beta-hydroxysteroid ... of 11β-hydroxysteroid dehydrogenase (11β-HSD). It has been known since 1987 that increased levels of 11β-OHP occur in 21- ... potent inhibitors of 11 beta-hydroxysteroid dehydrogenase (isoforms 1 and 2), confer marked mineralocorticoid activity on ... Souness GW, Latif SA, Laurenzo JL, Morris DJ (April 1995). "11 alpha- and 11 beta-hydroxyprogesterone, ...
Hastings C, Hansson V (August 1981). "Kinetic properties of the soluble 3 alpha-hydroxysteroid dehydrogenase from rat testis ... 5α-Pregnan-17α-ol-3,20-dione is produced by 5α-reduction of 17-OHP. The reaction is catalyzed by SRD5A1/SRD5A2 enzymes. ... 5α-Pregnan-17α-ol-3,20-dione, also known as 17α-hydroxy-dihydroprogesterone (17‐OH-DHP) is an endogenous steroid. 5α-Pregnan-17 ... The subsequent intermediate products in the pathway are 5α-pregnane-3α,17α-diol-20-one, androsterone and 5α-androstane-3α,17β- ...
20alpha-hydroxysteroid dehydrogenase MeSH D08.811.682.047.436.400.150 - cortisone reductase MeSH D08.811.682.047.485 - imp ... succinate dehydrogenase MeSH D08.811.682.660.900 - testosterone 5-alpha-Reductase MeSH D08.811.682.662.162 - dihydropteridine ... 11-beta-hydroxysteroid dehydrogenase type 1 MeSH D08.811.682.047.436.174.600 - 11-beta-hydroxysteroid dehydrogenase type 2 MeSH ... hydroxysteroid dehydrogenases MeSH D08.811.682.047.436.174 - 11-beta-hydroxysteroid dehydrogenases MeSH D08.811.682.047.436.174 ...
... has been reported to act as an inhibitor of 17β-hydroxysteroid dehydrogenases. 16-Ketoestrone can be converted ... Huffman MN, Lott MH (January 1948). "16-Substituted steroids; 16-keto-alpha-estradiol and 16-ketoestrone". J. Biol. Chem. 172 ( ... by 16α-hydroxysteroid dehydrogenase into estriol in the body. Breuer, Heinz (1962). "The Metabolism of the Natural Estrogens". ... "Affinity labeling of arginyl residues at the catalytic region of estradiol 17 beta-dehydrogenase from human placenta by 16- ...
11 beta hydroxysteroid dehydrogenase type 2 deficiency 17 alpha hydroxylase deficiency 17 beta hydroxysteroide dehydrogenase ... 46 xx gonadal dysgenesis epibulbar dermoid, rare (NIH) 47, XXY syndrome 47, XYY syndrome 47, XXX syndrome 48, XXXX syndrome 48 ... 3 alpha methylglutaconic aciduria, type 3, rare (NIH) 3 beta hydroxysteroid dehydrogenase deficiency 3 hydroxyisobutyric ... 3 alpha methylcrotonyl-Coa carboxylase 1 deficiency, rare (NIH) 3 alpha methylcrotonyl-coa carboxylase 2 deficiency, rare (NIH ...
Stomski FC, Sun Q, Bagley CJ, et al., Human interleukin-3 (IL-3) induces disulfide-linked IL-3 receptor alpha- and beta-chain ... Egli trovò un fattore derivato dalla cellula T che induceva la sintesi di 20alpha-idrossisteroido-deidrogenasi in cellule ... induces disulfide-linked IL-3 receptor alpha- and beta-chain heterodimerization, which is required for receptor activation but ... Ihle JN, Pepersack L, Rebar L, Regulation of T cell differentiation: in vitro induction of 20 alpha-hydroxysteroid ...
"Mechanism of inhibition of 3 alpha, 20 beta-hydroxysteroid dehydrogenase by a licorice-derived steroidal inhibitor". Structure ... Glucose/ribitol dehydrogenase InterPro: IPR002347 Insect alcohol dehydrogenase family InterPro: IPR002424 2,3-dihydro-2,3- ... alcohol dehydrogenases. Most members of this family are proteins of about 250 to 300 amino acid residues. Most dehydrogenases ... The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or ...
20alpha-dihydroxypregn-4-en-3-one, NAD+, and NADP+, whereas its 4 products are 17-alpha-hydroxyprogesterone, NADH, NADPH, and ... Other names in common use include 20alpha-hydroxy steroid dehydrogenase, 20alpha-hydroxy steroid dehydrogenase, 20alpha-HSD, ... "Expression of 17beta-hydroxysteroid dehydrogenase type 1 and type 2, P450 aromatase, and 20alpha-hydroxysteroid dehydrogenase ... Hershkovitz L, Beuschlein F, Klammer S, Krup M, Weinstein Y (March 2007). "Adrenal 20alpha-hydroxysteroid dehydrogenase in the ...
3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4)". Rizner TL, Lin HK, Peehl DM, Steckelbroeck S, ... "Close kinship of human 20alpha-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes". Genes to Cells. 5 (2): ... Khanna M, Qin KN, Cheng KC (June 1995). "Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular ... Steroidogenic enzyme 3α-hydroxysteroid dehydrogenase type 3 3β-Hydroxysteroid dehydrogenase GRCh38: Ensembl release 89: ...
... hydroxysteroid dehydrogenase (EC 1.1.1.210) is an enzyme that catalyzes the chemical reaction 5α-androstan-3β,17β-diol + NADP+ ... H+ This enzyme possesses the combined activities of the 3-β-hydroxysteroid dehydrogenase/Δ-5-4 isomerase and 20-α- ... hydroxysteroid dehydrogenase enzymes. Sharaf MA, Sweet F (September 1982). "Dual activity at an enzyme active site: 3 beta,20 ... alpha-hydroxysteroid oxidoreductase from fetal blood". Biochemistry. 21 (19): 4615-20. doi:10.1021/bi00262a016. PMID 6958329. ...
It is biosynthesized by 3β-hydroxysteroid dehydrogenase from progesterone. Unlike 3α-dihydroprogesterone (3α-DHP), 3β-DHP does ... 3 alpha HP)". Brain Research. 645 (1-2): 325-329. doi:10.1016/0006-8993(94)91667-5. ISSN 0006-8993. PMID 7914815. S2CID ... "Reduction of predator odor-induced anxiety in mice by the neurosteroid 3 alpha-hydroxy-4-pregnen-20-one ( ... In the Clauberg bioassay the 3β-hydroxy-4-pregnen-20-one shows about the same potency as progesterone (34). In regard to the ...
Casey ML, MacDonald PC, Andersson S (November 1994). "17 beta-Hydroxysteroid dehydrogenase type 2: chromosomal assignment and ... and AKR1C3 and the 17β-hydroxysteroid dehydrogenase (17β-HSD) HSD17B1. 20α-DHP can be transformed back into progesterone by 20α ... Pasqualini JR, Chetrite G (2008). "The anti-aromatase effect of progesterone and of its natural metabolites 20alpha- and 5alpha ... In animal studies, 20α-DHP has been found to be selectively taken up into and retained in target tissues such as the uterus, ...
"The identification of 5 alpha-reductase-2 and 17 beta-hydroxysteroid dehydrogenase-3 gene defects in male pseudohermaphrodites ... "Entrez Gene: HSD17B3 hydroxysteroid (17-beta) dehydrogenase 3". "Testosterone 17-beta-dehydrogenase deficiency - Conditions - ... 17β-Hydroxysteroid dehydrogenase 3 (17β-HSD3) is an enzyme that in humans is encoded by the HSD17B3 gene and is involved in ... 17β-Hydroxysteroid dehydrogenase GRCh38: Ensembl release 89: ENSG00000130948 - Ensembl, May 2017 GRCm38: Ensembl release 89: ...
"11beta-hydroxysteroid dehydrogenase type 1: a tissue-specific regulator of glucocorticoid response". Endocrine Reviews. 25 (5 ... Cortisol is also metabolized into 5-alpha tetrahydrocortisol (5-alpha THF) and 5-beta tetrahydrocortisol (5-beta THF), ... Cortisol is metabolized by the 11-beta hydroxysteroid dehydrogenase system (11-beta HSD), which consists of two enzymes: 11- ... and tumor necrosis factor alpha (TNF-alpha) by antigen-presenting cells (APCs) and T helper cells (Th1 cells), but upregulates ...
It is biosynthesized by 3α-hydroxysteroid dehydrogenase from progesterone. 3α-DHP has been found to act as a positive ... 3 alpha HP)". Brain Research. 645 (1-2): 325-329. doi:10.1016/0006-8993(94)91667-5. ISSN 0006-8993. PMID 7914815. S2CID ... "Reduction of predator odor-induced anxiety in mice by the neurosteroid 3 alpha-hydroxy-4-pregnen-20-one ( ... 3α-Dihydroprogesterone (3α-DHP), also known as 3α-hydroxyprogesterone, as well as pregn-4-en-3α-ol-20-one, is an endogenous ...
Marcus PI, Talalay P (February 1956). "Induction and purification of alpha- and beta-hydroxysteroid dehydrogenases". The ... 17β-Hydroxysteroid dehydrogenases (17β-HSD, HSD17B) (EC 1.1.1.51), also 17-ketosteroid reductases (17-KSR), are a group of ... Ning X, Yang Y, Deng H, Zhang Q, Huang Y, Su Z, Fu Y, Xiang Q, Zhang S (2017). "Development of 17β-hydroxysteroid dehydrogenase ... Soubhye J, Alard IC, van Antwerpen P, Dufrasne F (2015). "Type 2 17-β hydroxysteroid dehydrogenase as a novel target for the ...
This process is catalyzed by 3 α- and 3 β-hydroxy steroid dehydrogenase (HSD). CYP450 enzymes will then catalyze aromatic ... Alpha-zearalanol is also produced semisynthetically, for veterinary use; such use is prohibited in the European Union. ... More than one study has shown that detectable levels of zearalenone and its metabolite alpha-zearalanol in girls are associated ... Richardson, Kurt E.; Hagler, Winston M.; Mirocha, Chester J. (September 1985). "Production of zearalenone, .alpha.- and .beta.- ...
Conversion of androstenedione to testosterone requires the enzyme 17β-hydroxysteroid dehydrogenase. Androstenedione is released ... "DHEA metabolites activate estrogen receptors alpha and beta". Steroids. 78 (1): 15-25. doi:10.1016/j.steroids.2012.10.002. PMC ... with subsequent conversion of DHEA to androstenedione via the enzyme 3β-hydroxysteroid dehydrogenase. The secondary pathway ... "Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta". ...
... factor-5 and a GATA-like protein determine placental-specific expression of the Type I human 3beta-hydroxysteroid dehydrogenase ... is a transcriptional activator in alpha 1-adrenergic agonist-stimulated cardiac myocytes". The Journal of Biological Chemistry ... 272 (20): 12928-37. doi:10.1074/jbc.272.20.12928. PMID 9148898. "Entrez Gene: TEAD3 TEA domain family member 3". Bürglin, TR ( ...
Mutations in the genes encoding 11β-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase interact to cause ... Fukuoka M, Yasuda K, Fujiwara H, Kanzaki H, Mori T. Interactions between interferon gamma, tumour necrosis factor alpha, and ... 20 April 2011 [19 November 2011].. *^ 52.0 52.1 52.2 52.3 52.4 52.5 52.6 52.7 52.8 Richard Scott Lucidi. Polycystic Ovarian ... 2014, 20 (5): 688-701. PMID 24821925. doi:10.1093/humupd/dmu020.. *^ 69.0 69.1 Legro RS, Kunselman AR, Dodson WC, Dunaif A. ...
Phosphogluconate dehydrogenase. *Sorbitol dehydrogenase. *Hydroxysteroid dehydrogenase: 3β *3β-HSD. *NSDHL ... DCSR catalyzes the reduction of several L-xylylose as well as a number of pentoses, tetroses, trioses, alpha-dicarbonyl ...
17β-Hydroxysteroid dehydrogenase deficiency. *Aromatase excess syndrome. Androgen receptor. *Androgen insensitivity syndrome ... EIS results when the function of the estrogen receptor alpha (ERα) is impaired. The ERα protein (pictured) mediates most of the ... 20 (3): 358-417. doi:10.1210/edrv.20.3.0370. PMID 10368776.. *^ a b c Hill RA, Boon WC (2009). "Estrogens, brain, and behavior ... the estrogen receptor alpha (ERα) - that results in an inability of estrogen to mediate its biological effects in the body.[3] ...
11β-hydroxylase and 3β-hydroxysteroid dehydrogenase), lipoid CAH due to deficiency of StAR and mitochondrial DNA mutations.[8] ... The subunit ACTH undergoes further cleavage to produce alpha-MSH, the most important MSH for skin pigmentation. In secondary ... Diagnosis is made by a blood test to detect very long chain fatty acids.[20] ...
... involves a defect in the gene for 11β-hydroxysteroid dehydrogenase, an enzyme that normally inactivates circulating cortisol to ... 17 alpha-hydroxylase deficiency causes an inability to produce cortisol. Instead, extremely high levels of the precursor ... by causing inhibition of the 11β-hydroxysteroid dehydrogenase enzyme and likewise leading to secondary apparent ... "Impaired protein stability of 11beta-hydroxysteroid dehydrogenase type 2: a novel mechanism of apparent mineralocorticoid ...
... crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, alpha-ketomalonate and tetrahydoNAD". J Mol ... Phosphogluconate dehydrogenase. *Sorbitol dehydrogenase. *Hydroxysteroid dehydrogenase: 3β *3β-HSD. *NSDHL ... lactate dehydrogenase A. (subunit M). Human lactate dehydrogenase M4 (the isoenzyme found in skeletal muscle). From PDB: 1I10​. ... D-lactate dehydrogenase, membrane binding. crystal structure of d-lactate dehydrogenase, a peripheral membrane respiratory ...
17β-hydroxysteroid dehydrogenase deficiency. *aromatase excess syndrome. *Androgen receptor (Androgen insensitivity syndrome) ... Alpha-thalassemia mental retardation syndrome. *Siderius X-linked mental retardation syndrome. *Eye disorders: Color blindness ... The amount of urine produced can be nearly 20 liters per day.[1] Reduction of fluid has little effect on the concentration of ... Central DI usually starts between the ages of 10 and 20 and occurs in males and females equally.[2] Nephrogenic DI can begin at ...
... which regulates the expression of the enzyme 17β-hydroxysteroid dehydrogenase that is used to convert androstenedione, the ... The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids in human but 96 amino acids in almost all ... In contrast to the alpha gene activity, beta LH subunit gene activity is restricted to the pituitary gonadotropic cells. It is ... Each monomeric unit is a glycoprotein molecule; one alpha and one beta subunit make the full, functional protein. ...
The enzymes involved in these metabolic processes are 5α- and 5β-reductase as well as 3α- and 3β-hydroxysteroid dehydrogenase ( ... Chu YH, Li QA, Zhao ZF, Zhou YP, Cao DC (1985). "[Antiprogestational action of 5 alpha-dihydronorethisterone]". Zhongguo Yao Li ... and 3β-hydroxysteroid dehydrogenase both in the liver and in extrahepatic tissues such as the pituitary gland, uterus, prostate ... GREENBLATT RB (1956). "The progestational activity of 17-alpha-ethinyl-19-nortestosterone". J. Clin. Endocrinol. Metab. 16 (7 ...
The critical enzyme step is two-fold using a 3β-hydroxysteroid dehydrogenase and a Δ5-4 isomerase. The latter transfers the ... Pregnenolone (P5), or pregn-5-en-3β-ol-20-one, is an endogenous steroid and precursor/metabolic intermediate in the ... Pregnenolone is also known chemically as pregn-5-en-3β-ol-20-one.[27][28] Like other steroids, it consists of four ... InChI=1S/C21H32O2/c1-13(22)17-6-7-18-16-5-4-14-12-15(23)8-10-20(14,2)19(16)9-11-21(17,18)3/h4,15-19,23H,5-12H2,1-3H3/t15-,16-, ...
16α-OH-DHEA is converted by 3β-hydroxysteroid dehydrogenase type I (3β-HSD1) into 16α-hydroxyandrostenedione (16α-OH-A4) and 16 ... "Evaluation of ligand selectivity using reporter cell lines stably expressing estrogen receptor alpha or beta". Biochemical ... placental 17β-hydroxysteroid dehydrogenase interconverts estrone and estradiol and the two hormones are secreted into the ... DHEA is converted by 3β-hydroxysteroid dehydrogenase type I into androstenedione, and androstenedione is aromatized into ...
Zheng F (2010). "Methyltrienolone (R1881) is a Potent Inhibitor of 3B-Hydroxysteroid Dehydrogenase (3B-HSD) Activity". ... Krüskemper HL, Noell G (July 1966). "Liver toxicity of a new anabolic agent: methyltrienolone (17-alpha-methyl-4,9,11- ... metribolone was identified in 2010 as a potent inhibitor of 3β-hydroxysteroid dehydrogenase (3β-HSD) 1 and 2 (IC50 = 0.02 and ... InChI=1S/C19H24O2/c1-18-9-7-15-14-6-4-13(20)11-12(14)3-5-16(15)17(18)8-10-19(18,2)21/h7,9,11,16-17,21H,3-6,8,10H2,1-2H3/t16-, ...
Phosphogluconate dehydrogenase. *Sorbitol dehydrogenase. *Hydroxysteroid dehydrogenase: 3β *3β-HSD. *NSDHL ... renal (25-Hydroxyvitamin D3 1-alpha-hydroxylase or CYP27B1). *degradation (1,25-Dihydroxyvitamin D3 24-hydroxylase or CYP24A1) ... 20 (9): 1597-610. doi:10.1359/JBMR.050406. PMID 16059632.. *^ Lisko, Katherine; Raquel Torres; Rodney S. Harris; Melinda ... are a popular human model but lost the ability to synthesize L-gulono-gamma-lactone oxidase 20 million years ago.[6] This is a ...
Hydroxysteroid dehydrogenase. ... CYP27A1 (bile acid biosynthesis), CYP27B1 (vitamin D3 1-alpha ... CYP51A1 (lanosterol 14-alpha demethylase) P450s in other species[edit]. Animals[edit]. Many animals have as many or more CYP ... 7-alpha hydroxylation of 24-hydroxycholesterol. 1 subfamily, 1 gene. CYP39A1 CYP46. cholesterol 24-hydroxylase. 1 subfamily, 1 ... steroid biosynthesis, 17-alpha hydroxylase. 1 subfamily, 1 gene. CYP17A1 CYP19. steroid biosynthesis: aromatase synthesizes ...
"Chalcones are potent inhibitors of aromatase and 17beta-hydroxysteroid dehydrogenase activities". Life Sciences. 68 (7): 751-61 ... Om AS, Chung KW (April 1996). "Dietary zinc deficiency alters 5 alpha-reduction and aromatization of testosterone and androgen ... 20 (3): 277-84. doi:10.1055/s-2002-35374. PMID 12428207.. *. Carreau S, Bourguiba S, Lambard S, Galeraud-Denis I (2003). "[ ... 20] It is one of the causes of familial precocious puberty-a condition first described in 1937.[21] ...
... and oxidative 3α-hydroxysteroid dehydrogenase and 3β-hydroxysteroid dehydrogenase enzymes.[42] ... Lee JY, Cho KS (May 2018). "Effects of 5-alpha reductase inhibitors: new insights on benefits and harms". Curr Opin Urol. 28 (3 ... specifically with local expression at the skin of reductive 17β-hydroxysteroid dehydrogenase, ... Trost L, Saitz TR, Hellstrom WJ (2013). "Side Effects of 5-Alpha Reductase Inhibitors: A Comprehensive Review". Sex Med Rev. 1 ...
This is attributed to its high affinity as a substrate for 3α-hydroxysteroid dehydrogenase (3α-HSD), which is highly expressed ... Blackburn CM, Childs DS (March 1959). "Use of 2 alpha-methyl androstan-17 beta-ol, 3-one (2-methyl dihydrotestosterone) in the ... InChI=1S/C19H30O2/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(21)19(15,2)10-8-16(14)18/h12,14-17,21H,3-11H2,1-2H3/t12-,14-,15-,16 ... "Intramuscular administration of 5 alpha-dihydrotestosterone heptanoate: changes in urinary hormone profile". Clinical ...
17β-hydroxysteroid dehydrogenase deficiency. *aromatase excess syndrome. *Androgen receptor (Androgen insensitivity syndrome) ... Pure β-blockers do not inhibit lid-retraction in the eyes, which is mediated by alpha adrenergic receptors. ... 20 (11): 897-909. doi:10.2165/00023210-200620110-00003. PMID 17044727.. *^ Falgarone G, Heshmati HM, Cohen R, Reach G (January ... 14 (1): 107-20. doi:10.1210/er.14.1.107. PMID 8491150.. *^ Smith TJ, Hegedüs L (October 2016). "Graves' Disease". The New ...
... and oxidative 3α-hydroxysteroid dehydrogenase and 3β-hydroxysteroid dehydrogenase enzymes.[37] ... Trost L, Saitz TR, Hellstrom WJ (2013). "Side Effects of 5-Alpha Reductase Inhibitors: A Comprehensive Review". Sex Med Rev. 1 ... specifically with local expression at the skin of reductive 17β-hydroxysteroid dehydrogenase, ... "FDA Alert: 5-alpha reductase inhibitors (5-ARIs): Label Change - Increased Risk of Prostate Cancer". Drugs.com. Retrieved 2014- ...
Souness GW, Morris DJ (1996). "11 alpha- and 11 beta-hydroxyprogesterone, potent inhibitors of 11 beta-hydroxysteroid ... of 11β-hydroxysteroid dehydrogenase (11β-HSD). It is notably not metabolized by 11β-HSD2. 11α-OHP is a more potent inhibitor of ... 11 alpha-Hydroxyprogesterone (11 alpha OH-P) was an order of magnitude more potent a competitive inhibitor of the 11 beta HSD-2 ... "Human 11β-hydroxysteroid dehydrogenase: Studies on the stably transfected isoforms and localization of the type 2 isozyme ...
Marcus PI, Talalay P (February 1956). "Induction and purification of alpha- and beta-hydroxysteroid dehydrogenases". The ... 17β-Hydroxysteroid dehydrogenases (17β-HSD, HSD17B) (EC 1.1.1.51), also 17-ketosteroid reductases (17-KSR), are a group of ... Yang SY, He XY, Miller D (2011). "Hydroxysteroid (17β) dehydrogenase X in human health and disease". Mol. Cell. Endocrinol. 343 ... Mindnich R, Möller G, Adamski J (2004). "The role of 17 beta-hydroxysteroid dehydrogenases". Mol. Cell. Endocrinol. 218 (1-2): ...
In the final and rate limiting step, the C17 keto group androstenedione is reduced by 17β-hydroxysteroid dehydrogenase to yield ... Randall VA (Apr 1994). "Role of 5 alpha-reductase in health and disease". Baillière's Clinical Endocrinology and Metabolism. 8 ... 3α-hydroxysteroid dehydrogenase, and 17β-HSD, in that order.[1][147][148] Androsterone and etiocholanolone are then ... which regulates the expression of 17β-hydroxysteroid dehydrogenase.[124]. The amount of testosterone synthesized is regulated ...
The conversion of androstenedione to testosterone is catalyzed by 17β-hydroxysteroid dehydrogenase (17β-HSD), whereas the ... "DHEA metabolites activate estrogen receptors alpha and beta". Steroids. 78 (1): 15-25. doi:10.1016/j.steroids.2012.10.002. PMC ... estradiol is dehydrogenated by 17β-hydroxysteroid dehydrogenase into the much less potent estrogen estrone. These reactions ... "Discovery of estrogen receptor alpha target genes and response elements in breast tumor cells". Genome Biology. 5 (9): R66. ...
17β-hydroxysteroid dehydrogenase deficiency. *aromatase excess syndrome. *Androgen receptor (Androgen insensitivity syndrome) ... Alpha lipoic acid. *Benfotiamine. *Botulinum toxin A. *Bupropion. *Cannabinoids (e.g., cannabis, dronabinol, nabilone) ... Heat, therapeutic ultrasound,[20] hot wax[20] are also useful for treating diabetic neuropathy.[20] Pelvic floor muscle ... 20] Off-loading techniques can include the use of mobility aids (e.g. crutches) or foot splints.[20] Gait re-training would ...
17β-hydroxysteroid dehydrogenase deficiency. *aromatase excess syndrome. *Androgen receptor (Androgen insensitivity syndrome) ... Pure β-blockers do not inhibit lid-retraction in the eyes, which is mediated by alpha adrenergic receptors. ... 20 (11): 897-909. doi:10.2165/00023210-200620110-00003. PMID 17044727.. *^ Falgarone G, Heshmati HM, Cohen R, Reach G (2013). " ... Longo, Dan L.; Smith, Terry J.; Hegedüs, Laszlo (20 October 2016). "Graves' Disease". New England Journal of Medicine. 375 (16 ...
DHT is metabolized by 3α-hydroxysteroid dehydrogenase (3α-HSD) and 3β-hydroxysteroid dehydrogenase (3β-HSD) into 3α- ... Mutzebaugh C (1998). "Does the choice of alpha-AAS really make a difference?". HIV Hotline. 8 (5-6): 10-1. PMID 11366379.. ... AAS differ in their capacities to be metabolized by steroidogenic enzymes such as 5α-reductase, 3-hydroxysteroid dehydrogenases ... DHT, via its metabolite 3α-androstanediol (produced by 3α-hydroxysteroid dehydrogenase (3α-HSD)), is a neurosteroid that acts ...
20alpha-dihydroxypregn-4-en-3-one, NAD+, and NADP+, whereas its 4 products are 17-alpha-hydroxyprogesterone, NADH, NADPH, and ... Other names in common use include 20alpha-hydroxy steroid dehydrogenase, 20alpha-hydroxy steroid dehydrogenase, 20alpha-HSD, ... "Expression of 17beta-hydroxysteroid dehydrogenase type 1 and type 2, P450 aromatase, and 20alpha-hydroxysteroid dehydrogenase ... Hershkovitz L, Beuschlein F, Klammer S, Krup M, Weinstein Y (March 2007). "Adrenal 20alpha-hydroxysteroid dehydrogenase in the ...
... hydroxysteroid dehydrogenase (EC 1.1.1.210) is an enzyme that catalyzes the chemical reaction 5α-androstan-3β,17β-diol + NADP+ ... H+ This enzyme possesses the combined activities of the 3-β-hydroxysteroid dehydrogenase/Δ-5-4 isomerase and 20-α- ... hydroxysteroid dehydrogenase enzymes. Sharaf MA, Sweet F (September 1982). "Dual activity at an enzyme active site: 3 beta,20 ... alpha-hydroxysteroid oxidoreductase from fetal blood". Biochemistry. 21 (19): 4615-20. doi:10.1021/bi00262a016. PMID 6958329. ...
In vitro, 20 alpha SDH can be induced in splenic lymphocytes from nu/nu mice by conditioned media from mitogen- or alloantigen- ... The enzyme 20 alpha-hydroxysteroid dehydrogenase (20 alpha SDH) has previously been shown to be a specific enzyme marker of ... In vivo, the expression of 20 alpha SDH is thymus dependent, in that splenic lymphocytes from athymic mice have only low levels ... The factor responsible for 20 alpha SDH induction has been partially purified and is distinct from other known lymphokines in ...
... and that of 20alpha-hydroxysteroid dehydrogenase (20alphaHSD), involved in progesterone catabolism; and ii) to determine the ... the luteal metabolism of progesterone during PSP by measuring the activities of the enzymes 3beta-hydroxysteroid dehydrogenase ... Hedin L, Rodgers RJ, Simpson ER, Richards JS: Changes in content of cytochrome P450(17)alpha, cytochrome P450scc, and 3-hydroxy ... Luteal 3beta-hydroxysteroid dehydrogenase and 20alpha-hydroxysteroid dehydrogenase activities in the rat corpus luteum of ...
Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2) (PubMed:8994190). Also has 20-alpha-HSD activity. Uses ... 17-beta-hydroxysteroid dehydrogenase (NAD+) activity Source: RHEA. *17-beta-hydroxysteroid dehydrogenase (NADP+) activity ... 17-beta-hydroxysteroid dehydrogenas.... 17-beta-hydroxysteroid dehydrogenase type 1 (cDNA FLJ50217, highly similar to Estradiol ... 17-beta-hydroxysteroid dehydrogenase type 1Add BLAST. 327. Amino acid modifications. Feature key. Position(s). Description ...
dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III. pseudo-chlordecone ... Human 3-alpha hydroxysteroid dehydrogenase type 3 (3α-HSD3): the V54L mutation restricting the steroid alternative binding and ... Title: Human 3-alpha hydroxysteroid dehydrogenase type 3 (3α-HSD3): the V54L mutation restricting the steroid alternative ... This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene ...
3β-hydroxysteroid dehydrogenase deficiency, see 3-beta-hydroxysteroid dehydrogenase deficiency. *4 alpha aminobutyrate ... 46,XX sex reversal, see 46,XX testicular disorder of sex development ... 3-beta-hydroxysteroid dehydrogenase deficiency. *3-beta-hydroxysteroid dehydrogenase deficiency, see 3-beta-hydroxysteroid ... 3b-hydroxysteroid dehydrogenase deficiency, see 3-beta-hydroxysteroid dehydrogenase deficiency. *3beta-HSDH deficiency, see ...
To study the effects of WCF extract on the activities of ovarian 3 [3-hydroxy steroid dehydrogenase [3beta-HSD] responsible for ... Animals, Laboratory , Plant Extracts , Hydroxysteroid Dehydrogenases/drug effects , Ovary , Pregnancy, Animal , Rats, Sprague- ... Hydroxysteroid Dehydrogenases Limits: Animals Language: English Journal: Iran. J. Med. Sci. Year: 2004 ... Hydroxysteroid Dehydrogenases Limits: Animals Language: English Journal: Iran. J. Med. Sci. Year: 2004 ...
... hydroxysteroid dehydrogenase deficiency. 5. 3C syndrome. 6. 4-Alpha-hydroxyphenylpyruvate hydroxylase deficiency. 7. 46,XX ... 1. 17-Beta-hydroxysteroid dehydrogenase III deficiency. 2. 2-Hydroxyglutaricaciduria. 3. 3-M Syndrome. 4. 3?- ... 8. 46,XX testicular disorder of sex development. 9. Aagenaes syndrome. 10. Aase Smith syndrome. More causes » , Show All 3609 ...
... hydroxysteroid dehydrogenase deficiency. 5. 3C syndrome. 6. 4-Alpha-hydroxyphenylpyruvate hydroxylase deficiency. 7. 46,XX ... 1. 17-Beta-hydroxysteroid dehydrogenase III deficiency. 2. 2-Hydroxyglutaricaciduria. 3. 3-M Syndrome. 4. 3?- ... 8. 46,XX testicular disorder of sex development. 9. Aagenaes syndrome. 10. Aase Smith syndrome. More causes » , Show All 4466 ... Generalized Edema (20 causes) *more types...» See full list of 13 types for Edema Causes of General Symptom Types. Research the ...
20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the ... Aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II). PANTR ... indanol dehydrogenase activity Source: UniProtKB-EC. *trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity Source: ... "Molecular characterization of two monkey dihydrodiol dehydrogenases.". Higaki Y., Kamiya T., Usami N., Shintani S., Shiraishi H ...
... corticosteroid 11-beta-dehydrogenase isozyme 2 [EC:1.1.1.-] K05295 E1.1.1.149; 20alpha-hydroxysteroid dehydrogenase [EC:1.1. ... 3-oxo-5-alpha-steroid 4-dehydrogenase 2 [EC:1.3.1.22] K12345 SRD5A3; 3-oxo-5-alpha-steroid 4-dehydrogenase 3 [EC:1.3.1.22 1.3. ... dehydrogenase 1 15486 Hsd17b2; hydroxysteroid (17-beta) dehydrogenase 2 27400 Hsd17b6; hydroxysteroid (17-beta) dehydrogenase 6 ... 15483 Hsd11b1; hydroxysteroid 11-beta dehydrogenase 1 15484 Hsd11b2; hydroxysteroid 11-beta dehydrogenase 2 105349 Akr1c18; ...
Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody. *Type III 3-alpha-hydroxysteroid dehydrogenase antibody ... Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. ... The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then ...
Hydroxysteroid (17 beta) dehydrogenase 1 antibody. *MGC13814 antibody. *Placental 17 beta hydroxysteroid dehydrogenase antibody ... Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. ... PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific ...
3-alpha hydroxysteroid dehydrogenase, type II)), Authors: Hsueh Kung Lin. Published in: Atlas Genet Cytogenet Oncol Haematol. ... Increased expression of type 2 3alpha-hydroxysteroid dehydrogenase/type 5 17beta-hydroxysteroid dehydrogenase (AKR1C3) and its ... Localization of type 5 17beta-hydroxysteroid dehydrogenase, 3beta-hydroxysteroid dehydrogenase, and androgen receptor in the ... Immunoelectron microscopic localization of 3beta-hydroxysteroid dehydrogenase and type 5 17beta-hydroxysteroid dehydrogenase in ...
17-beta hydroxysteroid dehydrogenase 3 deficiency: MedlinePlus Genetics (National Library of Medicine) ... 5-alpha reductase deficiency: MedlinePlus Genetics (National Library of Medicine) * Aromatase deficiency: MedlinePlus Genetics ... 46,XX testicular disorder of sex development: MedlinePlus Genetics (National Library of Medicine) ...
Hydroxysteroid Dehydrogenase mRNA in Human Endometrium and Decidua. (2003) ...
1.1.1.149 20alpha-hydroxysteroid dehydrogenase. K00212 AKR1C1; 20alpha/3alpha-hydroxysteroid dehydrogenase / dihydrodiol ... Molecular cloning of two human liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with ... 1.1.1.357 3alpha-hydroxysteroid 3-dehydrogenase. K00212 AKR1C1; 20alpha/3alpha-hydroxysteroid dehydrogenase / dihydrodiol ... 20alpha/3alpha-hydroxysteroid dehydrogenase / dihydrodiol dehydrogenase [EC:1.1.1.149 1.1.1.357 1.3.1.20]. ...
Response-specific and ligand dose-dependent modulation of estrogen receptor (ER) alpha activity by ERbeta in the uterus. ... Impact of estrogen receptor beta on gene networks regulated by estrogen receptor alpha in breast cancer cells. ... Transcriptional profiling of estrogen-regulated gene expression via estrogen receptor (ER) alpha or ERbeta in human ... 20.. PHLDA1 expression is controlled by an estrogen receptor-NFκB-miR-181 regulatory loop and is essential for formation of ER+ ...
The human type II 17 beta-hydroxysteroid dehydrogenase gene encodes two alternatively spliced mRNA species. DNA Cell Biol. 1995 ... 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity / estradiol 17-beta-dehydrogenase activity / testosterone ... Expression cloning and characterization of human 17 beta-hydroxysteroid dehydrogenase type 2, a microsomal enzyme possessing 20 ... alpha-hydroxysteroid dehydrogenase activity. J Biol Chem. 1993 Jun 15;268(17):12964-9. [PubMed:8099587] ...
... alpha-hydroxysteroid dehydrogenase for neurosteroids and its inhibition by benzodiazepines" (pdf). Biol Pharm Bull. 25 (4): 441 ... Usami N; Yamamoto T; Shintani S; Ishikura S; Higaki Y; Katagiri Y; Hara A. (Apr 2002). "Substrate specificity of human 3(20) ...
Aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid ... Alcohol dehydrogenase 5 (class III), chi polypeptide. NM_000671. Gene Info. ADH7. Alcohol dehydrogenase 7 (class IV), mu or ... Aldehyde dehydrogenase 1 family, member A1. NM_000689. Gene Info. ALDH1A2. Aldehyde dehydrogenase 1 family, member A2. NM_ ... 3-alpha)-hydroxysteroid dehydrogenase). NM_001353. Gene Info. AKR1C2. ...
20alpha-hydroxysteroid dehydrogenase; 3-alpha hydroxysteroid dehydrogenase, type II; 3-alpha-HSD type 2; 3-alpha-HSD type II, ... Testosterone 17-beta-dehydrogenase 5; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type IIb 3-alpha hydroxysteroid ... 3-alpha hydroxysteroid dehydrogenase, type II); Chlordecone reductase homolog HAKRb; DD-3; Dihydrodiol dehydrogenase 3; ... 3-alpha-hydroxysteroid dehydrogenase type 2; Aldo-keto reductase family 1 member C18; Aldo-keto reductase family 1 member C3; ...
Deficiency of 3alpha-hydroxysteroid dehydrogenase. *Deficiency of 3-hydroxyacyl-CoA dehydrogenase. *Deficiency of 3- ... Deficiency of alpha- and beta-trypsin. *Deficiency of alpha-1,4-glucan-protein synthase ... DPD (dihydropyrimidine dehydrogenase deficiency) 277.6. *Edema, edematous 782.3. *. angioneurotic (allergic) (any site) (with ...
... alpha}$-HSD Activity;Progesterone;$20{\alpha}$-dihydroprogesterone;Rat Ovary; ... Changes in Ovarian and Placental 20α-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat - $20{\ ... alpha}$. -hydroxysteroid dehydrogenase activity in spontaneous Neoplasms in the dog and cat. J. Vet. Med. Sci. 53:549-552. ... alpha}$. -hydroxysteroid dehydro-genase in the control of progesterone synthesis preceding parturition and lactogenesis in the ...
Aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II). NM_003739. NM_001253908. NM_001253909 ... Aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid ... 3-alpha)-hydroxysteroid dehydrogenase). NM_001353. Gene Info. AKR1C2. ... Aldehyde dehydrogenase 1 family, member A1. NM_000689. Gene Info. ALK. Anaplastic lymphoma receptor tyrosine kinase. NM_004304 ...
Chlordecone Reductase; 3-Alpha Hydroxysteroid Dehydrogenase, Type I; Dihydrodiol Dehydrogenase 4 2 3 ... Close kinship of human 20alpha-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes. (PMID: 10672042) ... aldo-keto reductase family 1,member C4 (3-alpha hydroxysteroid dehydrogenase,type I),with high affinity for dihydrotestosterone ... Molecular cloning of two human liver 3 alpha- hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with ...
... hydroxysteroid dehydrogenase. Biochemca et Biophysica Acta. 1079. 112-118 (1991). *. Description. 「研究成果報告書概要(欧文)」より ... Growth Factors / Cytokine / Activin / Transforming Growth Factor-beta / Nerve growth factor / Tumor Necrosis Factor-alpha / ... hydroxysteroid dehydrogenase in luteotrophic and luteolytic processes during rat pseudopregnancy. Journal of Reproduction and ... and alpha- subunits in the early embryos and oviducts of different strains of mice. Journal of Reproduction and Development.38 ...
17Beta-hydroxysteroid dehydrogenase-3 deficiency: diagnosis, phenotypic variability, population genetics, and worldwide ... Gad YZ, Nasr H, Mazen I. 5 alpha-reductase deficiency in patients with micropenis. J Inherit Metab Dis. 1997 Mar. 20(1):95-101 ... Individuals with 17-beta hydroxysteroid dehydrogenase deficiency most often have female-appearing genitalia and, less often, ... Phenotypic variability in 17beta-hydroxysteroid dehydrogenase-3 deficiency and diagnostic pitfalls. Clin Endocrinol (Oxf). 2007 ...
Incomplete forms of 17 beta-hydroxysteroid dehydrogenase deficiency type 3 can cause micropenis, but the genitalia are most ... Deficiency of 5-alpha reductase. 5-alpha reductase peripherally converts testosterone to the more potent DHT, which is ... 17Beta-hydroxysteroid dehydrogenase-3 deficiency: diagnosis, phenotypic variability, population genetics, and worldwide ... The enzyme 17-beta-hydroxysteroid dehydrogenase normally converts androstenedione to testosterone. In the deficiency state, the ...
  • Proteomics-based identification of secreted protein dihydrodiol dehydrogenase 2 as a potential biomarker for predicting cisplatin efficacy in advanced NSCLC patients. (nih.gov)
  • Molecular cloning of two human liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with chlordecone reductase and bile-acid binder. (genome.jp)
  • Aldo-keto reductase family 1 member C1 also known as 20α-hydroxysteroid dehydrogenase , 3α-hydroxysteroid dehydrogenase , and dihydrodiol dehydrogenase 1/2 is an enzyme that in humans is encoded by the AKR1C1 gene . (wikidoc.org)
  • 20alpha-HSD has been initially described as a progesterone metabolizing enzyme of the ovary. (wikipedia.org)
  • On a functional level, ovarian 20alpha-HSD is actively involved in the control of progesterone homeostasis in pregnancy of rats and mice. (wikipedia.org)
  • Accordingly, in mice with targeted deletion of the 20alpha-HSD gene, progesterone blood concentration remain high throughout pregnancy which results in a delay of 2-4 days in parturition. (wikipedia.org)
  • Indicating that expression of 20alpha-HSD activity is mandatory for the induction of parturition through reduction of progesterone blood concentration. (wikipedia.org)
  • To study the effects of WCF extract on the activities of ovarian 3 [3-hydroxy steroid dehydrogenase [3beta-HSD] responsible for the synthesis of progesterone and 20 alpha-HSD responsible for its degradation in rats . (bvsalud.org)
  • The extract of WCF decreased the ovarian 3beta-HSD specific activity by 47%, serum progesterone concentration by 30% and the number of live embryos by 67%, but it had no effect on the specific activity of ovarian 20alpha-HSD. (bvsalud.org)
  • Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). (uniprot.org)
  • Selective and potent inhibitors of human 20alpha-hydroxysteroid dehydrogenase (AKR1C1) that metabolizes neurosteroids derived from progesterone. (genome.jp)
  • 1986. Microsonal $20{\alpha}$ -atiol and $20{\alpha}$ -hydroxysteroid dehydrogenase activity for progesterone in human placenta. (koreascience.or.kr)
  • 1970. The roles of pregn- 5-ene- $3{\beta}$ , $20{\alpha}$ -atiol and $20{\alpha}$ -hydroxysteroid dehydro-genase in the control of progesterone synthesis preceding parturition and lactogenesis in the rat. (koreascience.or.kr)
  • This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. (abnova.com)
  • This finding suggests that the 17alpha-hydroxylase/c17-20-lyase step, which converts progesterone to androstenedione, is inhibited by OP, and that the cholesterol side-chain cleavage and 3beta-hydroxysteroid dehydrogenase -isomerase steps, which convert cholesterol to pregnenolone and pregnenolone to progesterone, respectively, are other potential sites of OP action. (cdc.gov)
  • Dab (Limanda limanda) ovarian fragments were incubated in vitro with either [4,7-3H]pregnenolone or 17 alpha-hydroxy[1,2,6,7-3H]progesterone to investigate the pattern of steroidogenesis. (ualg.pt)
  • The antiserum was raised against 17,20 alpha-P. The 17,20 alpha-[3H]P label was produced by incubating place milt with 17 alpha-hydroxy [3H]progesterone. (ualg.pt)
  • Monkey 20α-hydroxysteroid dehydrogenase (20α-HSD) is a catabolic enzyme responsible for converting progesterone into biologically inactive 20α-hydroxyprogesterone, thereby playing a key role in the estrous cycle or pregnancy and allowing ovulation and parturition to occur in most mammalian animals. (biomedcentral.com)
  • The activity of 20α-HSD is known to be down-regulated by prolactin (PRL) [ 5 ], allowing for the maintenance of progesterone secretion during the first half of pregnancy. (biomedcentral.com)
  • 20α-HSD-deficient mice sustain high progesterone levels and present a delay in parturition of several days, demonstrating that 20α-HSD regulates parturition downstream of the PGF 2 α receptor in an essential and nonredundant manner. (biomedcentral.com)
  • AKR1C1 transfers progesterone to its inactive state or in other words catalyzes the reaction of 20-alpha-hydroxy progesterone (20-alpha-OHP). (prospecbio.com)
  • Estrogen receptor beta - an independent prognostic marker in estrogen receptor alpha and progesterone receptor-positive breast cancer? (townsendletter.com)
  • The enzyme 17-beta,10-alpha-hydroxysteroid dehydrogenase may play an important role in increasing the estrogen-progesterone ratio in fetal membranes at the time of parturition. (readabstracts.com)
  • A study examined the activity of 17-beta,10-alpha-hydroxysteroid dehydrogenase and its affect on the estrogen-progesterone ratio in fetal membranes. (readabstracts.com)
  • Estradiol is the most potent form of naturally occurring estrogen, and 20-alpha-dihydroprogesterone is a weak form of progesterone. (readabstracts.com)
  • AKR1C1, a member of the AKR1C subfamily (that shows both 20α- and 3α-HSD activities), plays a major role in progesterone metabolism and maintenance of pregnancy through the formation of progestin. (biomedcentral.com)
  • Human AKR1C1 (20α-HSD) has been functionally expressed in the fission yeast, and that this has enabled the resulting yeast strain to efficiently catalyze the reduction of progesterone to 20α-dihydroprogesterone. (biomedcentral.com)
  • In enzymology, a 3-β(or 20-α)-hydroxysteroid dehydrogenase (EC 1.1.1.210) is an enzyme that catalyzes the chemical reaction 5α-androstan-3β,17β-diol + NADP+ ⇌ {\displaystyle \rightleftharpoons } 17β-hydroxy-5α-androstan-3-one + NADPH + H+ This enzyme possesses the combined activities of the 3-β-hydroxysteroid dehydrogenase/Δ-5-4 isomerase and 20-α-hydroxysteroid dehydrogenase enzymes. (wikipedia.org)
  • Because concomitant exposure to the antioxidants alpha-tocopherol or ascorbate did not alter the inhibition of testosterone formation by higher OP concentrations, it does not appear that OP is acting as a pseudosubstrate for the generation of free radicals, which can damage P450 enzymes. (cdc.gov)
  • AdhD alcohol dehydrogenase from the thermophile Pyrococcus furiosus is one of the enzymes being engineered with these features by the Banta Lab. (proteopedia.org)
  • I. A histochemical study of delta-5-3-beta-and 20-alpha-hydroxysteroid dehydrogenases and enzymes of carbohydrate oxidation during the estrous cycle. (harvard.edu)
  • Breast tissue and cell lines contain the necessary enzymes for estrogen synthesis, including aromatase and 17beta-hydroxysteroid dehydrogenase (17beta-HSD). (hairloss-research.org)
  • Testosterone deficiency may be caused by a defect anywhere along the hypothalamic-pituitary-gonadal axis or a defect in peripheral androgen action (5-alpha reductase deficiency or PAIS). (medscape.com)
  • 11-Beta hydroxylase deficiency, 3-beta hydroxysteroid dehydrogenase deficiency, and 17 alpha-hydroxylase/17,20-lyase deficiency are generally not characterized by the development of hyperkalemia. (medscape.com)
  • while CAH forms which are associated with hypertension include 17[alpha]-hydroxylase and 11[beta]-hydroxylase deficiency. (thefreedictionary.com)
  • The V54L mutation significantly decreases the 3alpha-hydroxysteroid dehydrogenase activity of DDH2 for the reduction of dihydrotestosterone. (nih.gov)
  • Labrie Y, Durocher F, Lachance Y, Turgeon C, Simard J, Labrie C, Labrie F: The human type II 17 beta-hydroxysteroid dehydrogenase gene encodes two alternatively spliced mRNA species. (drugbank.ca)
  • Our results indicate that the Ap-1 site (-281 → -274) (5′-TGTCTCAT-3′) plays a crucial role in the activation of the monkey 20α-HSD gene. (biomedcentral.com)
  • Placental lactogen (PL) is another suppressor of 20α-HSD gene expression. (biomedcentral.com)
  • The 20alpha-HSD gene (from now on referred to as AKR1C1 ) cloned in this paper encodes a deletion of 4 amino acids, compared with the C-terminal region of AKR1C1 genes from other animals. (biomedcentral.com)
  • 1994. Identification of a major prolactin-regulated protein as 20 alpha-hydroxysteroid dehydrogenase: coordinate regulation of its activity, protein content, and messenger ribonucleic acid expression. (koreascience.or.kr)
  • These impairments concurred with the inhibited mRNA and protein levels of oestrogen receptor-alpha (Esr1) and 3β-hydroxysteroid dehydrogenase (HSD) in the adult uterus and/or ovaries. (mdpi.com)
  • Glucose chemotaxis is affected in specific TRPV subunit allele mutants: ocr-2(vs29) and osm-9(yz6), serotonin receptor mutants: ser-1(ok345) and mod-1(ok103), and G-alpha protein mutant: gpa-10(pk362). (unt.edu)
  • The AKR1C1 protein solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT and 20% glycerol. (prospecbio.com)
  • Genistein was shown to inhibit 5 alpha-reductase 2 in a non-competitive nature (Vmax and Km values without and with inhibitor were 30 and 20 pmol/mg protein per h and 177 and 170 nM respectively). (hairloss-research.org)
  • The porcine 20alpha-HSD cDNA is 957 bp in length and encodes a protein of 319 amino acids. (biomedcentral.com)
  • Testosterone is peripherally converted by the enzyme 5-alpha reductase to the more potent androgen DHT, which is responsible for virilization of the male external genitalia. (medscape.com)
  • The enzyme 17β-hydroxysteroid dehydrogenase type 2 (HSD17B2) plays a vital role in steroid metabolism, including the conversion of the biologically potent factor estradiol into the weakly estrogenic estrone in addition to testosterone and androstenedione [ 1 ]. (jcancer.org)
  • The conversion of androstenedione to testosterone was unaffected by exposure to OP, suggesting that the 17beta-hydroxysteroid dehydrogenase step is not inhibited. (cdc.gov)
  • HSD17B1(17-BETA-HYDROXYSTEROID DEHYDROGENASE I),also known as 17-beta-hydroxysteroid dehydrogenase or 17-ketosteroid reductase,is an enzyme that is responsible for the interconversion of estrone (E1) and estradiol (E2) as well as the interconversion of androstenedione and testosterone. (acris-antibodies.com)
  • Intratesticular testosterone concentrations were extremely low in hypophysectomized rats 15-20 days after surgery. (ulpgc.es)
  • The Conversion of Testosterone to 5 .alpha. (regrowth.com)
  • Azasteroids as Inhibitors of Testosterone 5 Alpha-Redustase in Mammalian Skin," J. Steroid Biochem. (regrowth.com)
  • AKR1C1, AKR1C2, and AKR1C3 AKR1C1 has high catalytic efficiency as a 20α-HSD and AKR1C2 and AKR1C3 efficiently catalyze this reaction as well. (wikipedia.org)
  • 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) (AKR1C1), mRNA. (abnova.com)
  • AKR1C1 Human Recombinant fused to a 20 amino acid His Tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 343 amino acids (1-323 a.a.) and having a molecular mass of 38.9 kDa. (prospecbio.com)
  • The AKR1C1 is fused to a 20 a.a. (prospecbio.com)
  • Monkey 20α-HSD was highly abundant in ovarian and placental tissues during the pre-ovulation and pre-parturition phase and was primarily localized in the syncytiotrophoblast of the placenta. (biomedcentral.com)
  • Human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase. (wikipedia.org)
  • Immunohistochemical Distribution of Aromatase and 3B-hydroxysteroid Dehydrogenase in Human Hair Follicle and Sebaceous Gland," J. Cutan. (regrowth.com)
  • Mammalian lignans and genistein decrease the activities of aromatase and 17beta-hydroxysteroid dehydrogenase in MCF-7 cells. (hairloss-research.org)
  • 1989. Purification and characterization of pig adrenal $20{\alpha}$ -hydroxysteroid dehydro-genage. (koreascience.or.kr)
  • [4] Another study with 46, XY individuals with androgen secreting testicular tissue and 46, XX individuals with congenital adrenal hyperplasia concluded that androgen exposure may have some effect in higher brain function and the perception of the gender. (nih.gov)
  • Human 3-alpha hydroxysteroid dehydrogenase type 3 (3α-HSD3): the V54L mutation restricting the steroid alternative binding and enhancing the 20α-HSD activity. (nih.gov)
  • Wu L, Einstein M, Geissler WM, Chan HK, Elliston KO, Andersson S: Expression cloning and characterization of human 17 beta-hydroxysteroid dehydrogenase type 2, a microsomal enzyme possessing 20 alpha-hydroxysteroid dehydrogenase activity. (drugbank.ca)
  • To identify associations between 17β-hydroxysteroid dehydrogenase type 2 (HSD17B2) expression and clinicopathological variables and prognoses in patients with urothelial carcinoma of the urinary tract. (jcancer.org)
  • 17β-hydroxysteroid dehydrogenase type 2, Urothelial carcinoma, Prognosis. (jcancer.org)
  • Quantitative RT-PCR confirmed the downregulation of three high-interest candidate genes which are all components of the extracellular matrix: proteoglycan 4 (PRG4), fibulin-1 (FBLN-1) transcript variant D, and type XV collagen alpha 1 chain. (biomedcentral.com)
  • Human 3-alpha hydroxysteroid dehydrogenase type 3 (3? (nih.gov)
  • 11b-hydroxysteroid dehydrogenase type 1: A tissue specific regulator of glucocorticoid response. (townsendletter.com)
  • Inhibition of rat ovarian 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), 17 alpha-hydroxylase and 17,20 lyase by progestins and danazol. (semanticscholar.org)
  • 1992. Changes of two kinds of $20{\alpha}$ - hydroxysteroid dehydrogenase during luteal phase in the rat. (koreascience.or.kr)
  • Hydroxysteroid Dehydrogenase mRNA in Human Endometrium and Decidua. (nii.ac.jp)
  • In enzymology, a 20-α-hydroxysteroid dehydrogenase (EC 1.1.1.149) is an enzyme that catalyzes the chemical reaction 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ ⇌ {\displaystyle \rightleftharpoons } 17alpha-hydroxyprogesterone + NAD(P)H + H+ The 3 substrates of this enzyme are 17alpha,20alpha-dihydroxypregn-4-en-3-one, NAD+, and NADP+, whereas its 4 products are 17-alpha-hydroxyprogesterone, NADH, NADPH, and H+. (wikipedia.org)
  • abstract = "The steroids, 17β-[1(R)-1-hydroxy-2-propynyl]androst-4-en-3-one (α-HPA) and 17β-(1-oxo-2-propynyl)-androst-4-en-3-one (OPA), were used to investigate the 17β-estradiol dehydrogenase and 20α-hydroxysteroid dehydrogenase activities which co-exist in the homogeneous enzyme purified from human placental cytosol. (wustl.edu)
  • Substrate specificity of human 3(20)alpha-hydroxysteroid dehydrogenase for neurosteroids and its inhibition by benzodiazepines" (pdf). (wikipedia.org)
  • Inhibition of 5 alpha-reductase in genital skin fibroblasts and prostate tissue by dietary lignans and isoflavonoids. (hairloss-research.org)
  • This report describes the inhibition of 5 alpha-reductase and 17 beta-hydroxysteroid dehydrogenase by six isoflavonoids and two lignans in human genital skin fibroblast monolayers and homogenates, and in benign prostatic hyperplasia tissue homogenates. (hairloss-research.org)
  • In genital skin fibroblasts, genistein, biochanin A and equol were the most potent inhibitors of 5 alpha-reductase activity, each resulting in greater than 80% inhibition at a concentration of 100 microM. (hairloss-research.org)
  • The systematic name of this enzyme class is 20alpha-hydroxysteroid:NAD(P)+ 20-oxidoreductase. (wikipedia.org)
  • 1981. Purification of $20{\alpha}$ hydroxysteroid oxidoreductase from bovine fetal erythrocytes. (koreascience.or.kr)
  • HSD17B2(17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. (acris-antibodies.com)
  • Pregnane-4,20-Dione in Female Rats. (semanticscholar.org)
  • Sixty-four pregnant rats were divided into 4 groups and exposed to feed contaminated with ZEN (0, 5, 10, and 20 mg/kg feed) on gestational days (GDs) 0-21. (mdpi.com)
  • Compared with the controls, the groups exposed to 10 and 20 mg/kg ZEN showed significantly decreased feed intake and body weight of pregnant rats and/or female offspring. (mdpi.com)
  • Meanwhile, 20 mg/kg ZEN significantly decreased the birth weight and viability of F1 newborn rats. (mdpi.com)
  • Moreover, 10 and 20 mg/kg ZEN diets increased follicle-stimulating hormone concentrations but decreased oestradiol in both maternal and F1 adult rats. (mdpi.com)
  • In the F1 generation, ZEN caused no pathological changes in ovaries and uterus in weaned rats, but significant follicular atresia and a thinning uterine layer were found in F1 female adult rats in the 20 mg/kg ZEN group. (mdpi.com)
  • Additionally, 20 mg/kg ZEN significantly upregulated ABCc5 expression in the placenta and ovaries of weaned rats. (mdpi.com)
  • This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. (nih.gov)
  • While 20alpha-HSD expression and activity is downregulated in the corpus luteum of pregnancy, 24 hrs prior to parturition ovarian 20alpha-HSD activity is acutely stimulated. (wikipedia.org)
  • In vivo, the expression of 20 alpha SDH is thymus dependent, in that splenic lymphocytes from athymic mice have only low levels of activity, although the levels of enzyme activity increase gradually with age. (jimmunol.org)
  • Beginning after a lag period of approximately 6 hr, enzyme activity increases linearly for approximately 20 to 30 hr resulting in a 5- to 10-fold increase in activity. (jimmunol.org)
  • Also has 20-alpha-HSD activity. (uniprot.org)
  • Also has some 20-alpha-hydroxysteroid dehydrogenase activity. (genecards.org)
  • Thus, we demonstrated that monkey 20α-HSD promoter activity is regulated by the transcription factor Ap-1 in CHO-K1 cells. (biomedcentral.com)
  • The simultaneous inactivation of both the major 17β-estradiol dehydrogenase and 20α-hydroxysteroid dehydrogenase activities by OPA and by enzymatic oxidation of α-HPA clearly demonstrates the bifunctional activity of the single enzyme-active site. (wustl.edu)
  • All of the compounds tested inhibited 17 beta-hydroxysteroid dehydrogenase activity in genital skin fibroblast monolayers. (hairloss-research.org)
  • Increased 5 alpha-reductase activity and adrenocortical drive in women with polycystic ovary syndrome. (townsendletter.com)
  • The activity of 17-beta,10-alpha-hydroxysteroid dehydrogenase changed at the time of parturition. (readabstracts.com)
  • Around this time, its activity resulted in the net production of estradiol and 20-alpha-dihydroprogesterone. (readabstracts.com)
  • The phenotype of both the precursor and the induced lymphocyte populations is Thy 1.2-, Lyt 1-, 2-, suggesting that induction of 20 alpha SDH expression is an early step in T cell differentiation. (jimmunol.org)
  • Expression of 20.ALPHA. (nii.ac.jp)
  • Tissue Distribution and Ontogeny of Steroid 5 Alpha-Redustase Isozyme Expression," J. Clin. (regrowth.com)
  • Reverse-transcriptase-PCR (RT-PCR), real-time PCR, northern blot analysis, and western blot analysis were performed to examine the expression of porcine 20alpha-HSD. (biomedcentral.com)
  • Furthermore, 10 and/or 20 mg/kg ZEN exposure significantly reduced Esr1, gonadotropin-releasing hormone receptor (GnRHr), and ATP binding cassette transporters b1 and c1 (ABCb1 and ABCc1) in the placenta and foetal and weaned F1 brains, and also produced a dose-dependent increase in 3β-HSD in the placenta. (mdpi.com)
  • A major enzyme found in the dab ovary was 20 alpha-hydroxysteroid dehydrogenase. (ualg.pt)
  • In this study, we focused on the molecular characterization of the monkey 20α-HSD promoter region by conducting reporter assays in Chinese hamster ovary (CHO) K1 cells. (biomedcentral.com)
  • Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. (nih.gov)
  • 1994. Molecular cloning of cDNA for rat ovarian 20 alpha-hydroxysteroid dehydrogenase (HSD1). (koreascience.or.kr)
  • 11beta,21-Dihydroxy-3,20-oxo-5beta-pregnan-18-al is an intermediate in C21-Steroid hormone metabolism. (hmdb.ca)
  • These results suggest that OP is a hormonally active agent, but that some of its actions are distinct from those of 17beta-estradiol and are not mediated through the estrogen receptor alpha or beta pathway. (cdc.gov)
  • Estrogens signal through two nuclear receptors: estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ). (frontiersin.org)
  • The radioimmunoassay was shown to have a high cross-reaction with the 5 beta-reduced analogues of 17,20 alpha-P and was therefore used, in conjunction with thin-layer chromatography, to measure the steroids. (ualg.pt)
  • Reaction of cyanuric fluoride with ribonuclease, alpha-lactalbumin, and beta-lactoglobulin. (naver.com)
  • Regulation of T cell differentiation: in vitro induction of 20 alpha-hydroxysteroid dehydrogenase in splenic lymphocytes from athymic mice by a unique lymphokine. (jimmunol.org)
  • 1990. Participation of ovarian $20{\alpha}$ -hydroxysteroid dehydrogenase in luteotrophic and luteolytic processes during rat pseudopregnancy. (koreascience.or.kr)
  • Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). (genecards.org)
  • Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). (nih.gov)
  • 11beta,21-Dihydroxy-3,20-oxo-5beta-pregnan-18-al is converted from Aldosterone via the enzyme 3-oxo-5beta-steroid 4-dehydrogenase (EC:1.3.99.6). (hmdb.ca)
  • It is then converted to 3alpha,11beta,21-Trihydroxy-20-oxo-5beta-pregnan-18-al via the enzyme 3-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.50). (hmdb.ca)