2',5'-Oligoadenylate Synthetase: An enzyme that catalyzes the conversion of ATP into a series of (2'-5') linked oligoadenylates and pyrophosphate in the presence of double-stranded RNA. These oligonucleotides activate an endoribonuclease (RNase L) which cleaves single-stranded RNA. Interferons can act as inducers of these reactions. EC 2.7.7.-.Oligoribonucleotides: A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Interferons: Proteins secreted by vertebrate cells in response to a wide variety of inducers. They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions.Porifera: The phylum of sponges which are sessile, suspension-feeding, multicellular animals that utilize flagellated cells called choanocytes to circulate water. Most are hermaphroditic. They are probably an early evolutionary side branch that gave rise to no other group of animals. Except for about 150 freshwater species, sponges are marine animals. They are a source of ALKALOIDS; STEROLS; and other complex molecules useful in medicine and biological research.Adenine NucleotidesEndoribonucleases: A family of enzymes that catalyze the endonucleolytic cleavage of RNA. It includes EC 3.1.26.-, EC 3.1.27.-, EC 3.1.30.-, and EC 3.1.31.-.RNA, Double-Stranded: RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.Enzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.Interferon-alpha: One of the type I interferons produced by peripheral blood leukocytes or lymphoblastoid cells. In addition to antiviral activity, it activates NATURAL KILLER CELLS and B-LYMPHOCYTES, and down-regulates VASCULAR ENDOTHELIAL GROWTH FACTOR expression through PI-3 KINASE and MAPK KINASES signaling pathways.Interferon Type I: Interferon secreted by leukocytes, fibroblasts, or lymphoblasts in response to viruses or interferon inducers other than mitogens, antigens, or allo-antigens. They include alpha- and beta-interferons (INTERFERON-ALPHA and INTERFERON-BETA).eIF-2 Kinase: A dsRNA-activated cAMP-independent protein serine/threonine kinase that is induced by interferon. In the presence of dsRNA and ATP, the kinase autophosphorylates on several serine and threonine residues. The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis.Interferon-beta: One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Antiviral Agents: Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cell Line: Established cell cultures that have the potential to propagate indefinitely.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Glutamate-Ammonia Ligase: An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.Amino Acyl-tRNA Synthetases: A subclass of enzymes that aminoacylate AMINO ACID-SPECIFIC TRANSFER RNA with their corresponding AMINO ACIDS.Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Kinetics: The rate dynamics in chemical or physical systems.Poly I-C: Interferon inducer consisting of a synthetic, mismatched double-stranded RNA. The polymer is made of one strand each of polyinosinic acid and polycytidylic acid.Peptide Synthases: Ligases that catalyze the joining of adjacent AMINO ACIDS by the formation of carbon-nitrogen bonds between their carboxylic acid groups and amine groups.Coenzyme A Ligases: Enzymes that catalyze the formation of acyl-CoA derivatives. EC 6.2.1.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
*  2'-5'-oligoadenylate synthase
... oligoadenylate synthetase is an enzyme. It is an antiviral enzyme that counteracts viral attack by degrading viral and host RNA ... oligoadenylate synthetase, also referred to as domain 2 of the enzyme, is largely alpha-helical and homologous to a tandem ... oligoadenylate synthetase". Mol. Cell. 12 (5): 1173-85. doi:10.1016/S1097-2765(03)00433-7. PMID 14636576. This article ... oligoadenylate synthetases. Structure-function relationships". J. Biol. Chem. 266 (23): 15293-9. PMID 1651324. Hartmann R, ...
*  Oasl2
... oligoadenylate synthetase-like 2 is a protein that in the house mouse is encoded by the Oasl2 gene. The gene is also known as ... oligoadenylate synthetase with a ubiquitin-like extension, is induced during maturation of murine dendritic cells". Journal of ... oligoadenylate synthetase-like 2". Retrieved 2011-12-11. "Salmonella infection data for Oasl2". Wellcome Trust Sanger Institute ... oligoadenylate synthetase gene family". Molecular Biology and Evolution. 17 (5): 738-750. doi:10.1093/oxfordjournals.molbev. ...
*  OASL
... oligoadenylate synthetase-like protein is an enzyme that in humans is encoded by the OASL gene. 2'-5'-oligoadenylate synthase ... oligoadenylate synthetase-like". Mackay V, Linn S (1976). "Selective inhibition of the dnase activity of the recBC enzyme by ... oligoadenylate synthetase-like gene (OASL) encoding the interferon-induced 56-kDa protein maps to chromosome 12q24.2 in the ... oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1". Eur. J. ...
*  OAS3
... oligoadenylate synthetase 3 is an enzyme that in humans is encoded by the OAS3 gene. This gene encodes an enzyme included in ... oligoadenylate synthetase". Genomics. 70 (2): 232-40. doi:10.1006/geno.2000.6382. PMID 11112351. Ito M, Shichijo S, Tsuda N, et ... oligoadenylate synthetase locus is composed of three distinct genes clustered on chromosome 12q24.2 encoding the 100-, 69-, and ... oligoadenylate synthetase catalyzing preferentially the synthesis of dimeric pppA2'p5'A molecules is composed of three ...
*  OAS1
... oligoadenylate synthetase 1 is an enzyme that in humans is encoded by the OAS1 gene. This gene encodes a member of the 2-5A ... oligoadenylate synthetase 1, 40/46kDa". Justesen J, Hartmann R, Kjeldgaard NO (2000). "Gene structure and function of the 2'-5 ... oligoadenylate synthetase". J. Biochem. 99 (5): 1455-64. PMID 3754863. Merlin G, Chebath J, Benech P, et al. (1983). "Molecular ... oligoadenylate synthetase is impaired by specific mutations that affect oligomerization of the protein". J. Biol. Chem. 272 (52 ...
*  Ribonuclease L
Oligoadenylate Synthetase (OAS). The transcribed RNA is then spliced and modified in the nucleus before reaching the cytoplasm ... oligoadenylate synthetase-dependent ribonuclease - is an interferon (IFN)-induced ribonuclease which, upon activation, destroys ... linked oligoadenylates (2-5A), which are 5' end phosphorylated. 2-5 A molecules then bind to RNase L, promoting its activation ... oligoadenylate depends on the post-transcriptional and post-translational modifications of OAS. OAS is only activated under a ...
*  OAS2
... oligoadenylate synthetase 2 is an enzyme that in humans is encoded by the OAS2 gene. This gene encodes a member of the 2-5A ... oligoadenylate synthetase 2, 69/71kDa". Justesen J, Hartmann R, Kjeldgaard NO (2000). "Gene structure and function of the 2'-5 ... oligoadenylate synthetase locus is composed of three distinct genes clustered on chromosome 12q24.2 encoding the 100-, 69-, and ... oligoadenylate synthetase family". Cell. Mol. Life Sci. 57 (11): 1593-1612. doi:10.1007/PL00000644. PMID 11092454. Marié I, ...
*  IFIT1
... oligoadenylate synthetase". Eur. J. Biochem. 257 (2): 319-30. doi:10.1046/j.1432-1327.1998.2570319.x. PMID 9826176. Guo J, Sen ... 267 (2): 209-19. doi:10.1006/viro.1999.0135. PMID 10662616. Guo J, Hui DJ, Merrick WC, Sen GC (2001). "A new pathway of ... 13 (2): 458-60. doi:10.1016/0888-7543(92)90272-T. PMID 1377167. Wathelet MG, Clauss IM, Content J, Huez GA (Jun 1988). "The IFI ... 169 (2): 313-21. doi:10.1111/j.1432-1033.1987.tb13614.x. PMID 3121313. Wathelet MG, Szpirer J, Nols CB, et al. (1988). "Cloning ...
*  Innate immune system
... oligoadenylate synthetase family, which degrades viral RNA. Some viruses evade this by producing molecules which interfere with ... 5 (6): 1003-1011. doi:10.1016/S1097-2765(00)80265-8. PMID 10911994. Rojo, E.; et al. (2004). "VPEgamma exhibits a caspase-like ... 5 (4): 331-42. doi:10.1038/nri1594. PMID 15803152. Janeway, C.A.; Travers, P.; Walport, M.; Shlomchik, M.J. (2005). ... 117 (2): 289-96. doi:10.1172/JCI30555. PMC 1783813 . PMID 17273548. Tracey, KJ (2009). "Reflex control of immunity". Nature ...
*  Interleukin 28
... oligoadenylate synthetase as well as ISGF3G (Interferon Stimulated Gene Factor 3). IL-28A and IL-28B belong to the type III ... 17 (2): 103-6. PMID 15171810. Sheppard P, Kindsvogel W, Xu W, Henderson K, Schlutsmeyer S, Whitmore TE, Kuestner R, Garrigues U ...
*  List of MeSH codes (D08)
... oligoadenylate synthetase MeSH D08.811.913.696.445.650 --- polynucleotide adenylyltransferase MeSH D08.811.913.696.445.692 --- ... amino acyl-trna synthetases MeSH D08.811.464.263.200.050 --- alanine-tRNA ligase MeSH D08.811.464.263.200.100 --- arginine-tRNA ... atp synthetase complexes MeSH D08.811.913.696.650.150.500 --- proton-translocating atpases MeSH D08.811.913.696.650.150.500.249 ... fatty acid synthetase complex MeSH D08.811.600.391 --- glycine decarboxylase complex MeSH D08.811.600.391.100 --- ...
*  RNA transfection
... members of the oligoadenylate synthetase family of proteins (OAS1, OAS2, OAS3), and others. All of these proteins can ... 23 (2): 165-175. doi:10.1016/j.immuni.2005.06.008. PMID 16111635. Diebold SS, Kaisho T, Hemmi H, Akira S, Reis e Sousa C (2004 ... 29 (2): 178-181. doi:10.1016/j.immuni.2008.07.009. PMID 18701081. Schmidt A, Schwerd T, Hamm W, Hellmuth JC, Cui S, et al. ( ... 5 (7): 730-737. doi:10.1038/ni1087. PMID 15208624. Das HK, Das A, Ghosh-Dastidar P, Ralston RO, Yaghmai B, et al. (1981). " ...
*  Polynucleotide adenylyltransferase
... synthetase Polyadenylate nucleotidyltransferase Polyadenylate polymerase Polyadenylate synthetase Polyadenylic acid polymerase ... Mans RJ, Walter TJ (1971). "Transfer RNA-primed oligoadenylate synthesis in maize seedlings. II Primer, substrate and metal ... U.S.A. 69 (2): 417-21. doi:10.1073/pnas.69.2.417. PMC 426470 . PMID 4501121. Colgan DF, Murthy KG, Prives C, Manley JL ( ... 384 (6606): 282-5. doi:10.1038/384282a0. PMID 8918882. Molecular and Cellular Biology portal. ...
Spontaneous production of the interferon induced enzyme 2,5-oligoadenylate synthetase (2,5-A synthetase) was significantly greater in blood lymphocytes of patients with systemic lupus erythematosus (SLE), Behçets syndrome, and rheumatoid arthritis than in control lymphocytes. Alpha interferon (IFN-alpha) induced comparable production of enzyme in normal, rheumatoid, and Behçets lymphocytes, and reduced, but still appreciable amounts in SLE lymphocytes. Phytohaemagglutinin (PHA) did not induce enzyme. Thus spontaneous production of 2,5-A synthetase is encountered in several connective tissue diseases and seems likely to be induced by in-vivo exposure to interferons. ...
The interferon inducible anti-viral 2-5 oligoadenylate synthetase (OAS) enzyme is part of the cellular innate immune response [1-3]. There are eight to ten OAS isoforms due to alternative splicing and gene duplications [4, 5]. In humans, the OAS gene family is composed of four genes on locus 12q24.1-q24.2: hOAS1, hOAS2, hOAS3 and hOASL [6, 7]. A pathogen-associated molecular pattern (PAMPs) such as dsRNA or mRNA with significant double stranded structures stimulates OAS to catalyze the polymerization of ATP to form 2-5-linked oligomers of adenylate (2-5As) [4, 8]. The subsequent elongation reaction is (n + 1) ATP → Ppp5A(2p5A)n + nPPi [9]. 2-5A functions through its receptor, the 2-5A-dependent ribonuclease (RNaseL), a ubiquitous 83-kDa protein that dimerizes into its catalytically active form upon binding 2-5A [4, 10, 11]. The activated RNaseL cleaves the single stranded RNA with UU or UA dinucleotide at the 3 end [4, 8, 12, 13]. Thus activated OAS-RNaseL system degrades ...
Previous studies from our laboratory have shown that deletion of residues 321 to 344 of the 9-2 isozyme of 2-5-oligoadenylate (2-5(A)) synthetase causes a loss of its enzyme activity (Ghosh, S. K., Kusari, J., Bandyopadhyay, S. K., Samanta, H., Kumar, R., and Sen, G. C. (1991) J. Biol. Chem. 266, 15293-15299). Sequence comparison of this region among the different isozymes of 2-5(A) synthetases revealed that the residues at positions 330 to 333 are highly conserved. Alanine-scanning mutagenesis of these residues demonstrated that the residues present at 331, 332, and 333 are important for activity but the proline at position 330 was dispensable. The triple mutant containing Ala residues at 331, 332, and 333 was completely inactive. Different double mutants were slightly active, and the three single mutants were partially active. The triple mutant was further characterized for delineating the nature of its defect. The mutant protein was enzymatically inactive ...
OAS1 Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 384 amino acids (1-364 a.a.) and having a molecular mass of 43.9kDa. The OAS1 is purified by proprietary c
Upregulates the expression of MHC I proteins, allowing for increased presentation of peptides derived from viral antigens. This enhances the activation of CD8+ T cells that are the precursors for cytotoxic T lymphocytes (CTLs) and makes the macrophage a better target for CTL-mediated killing. Interferon alpha also induce the synthesis of several key antiviral mediators, including 2-5 oligoadenylate synthetase (2-5 A synthetase) and protein kinase R ...
Upregulates the expression of MHC I proteins, allowing for increased presentation of peptides derived from viral antigens. This enhances the activation of CD8+ T cells that are the precursors for cytotoxic T lymphocytes (CTLs) and makes the macrophage a better target for CTL-mediated killing. Interferon alpha also induce the synthesis of several key antiviral mediators, including 2-5 oligoadenylate synthetase (2-5 A synthetase) and protein kinase R ...
The 5′-triphosphorylated, 2′-5′-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system. The 2-5As bind and activate the RNase L, an endoRNase degrading viral and cellular RNA leading to inhibition of viral replication. The 2-5A system is tightly controlled by synthesis and degradation of 2-5As. Whereas synthesis is mediated by the 2′-5′ oligoadenylate synthetase family of enzymes, degradation seems to be orchestrated by multiple enzyme nucleases including phosphodiesterase 12, the ectonucleotide pyrophosphatase/phosphodiesterase 1 and the A-kinase anchoring protein 7. Here we present assay tools for identification and characterization of the enzymes regulating cellular 2-5A levels. A procedure is described for the production of 2′-5′ oligoadenylates, which are then used as substrates for development and demonstration of enzyme assays measuring synthetase and nuclease ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
The present invention provides methods for synthesis and therapeutic use of DNA and RNA oligonucleotides and analogs. RNA oligonucleotides are synthesized using a small, circular DNA template which lacks an RNA polymerase promoter sequence. The RNA synthesis is performed by combining a circular single-stranded oligonucleotide template with an effective RNA polymerase and at least two types of ribonucleotide triphosphate to form an RNA oligonucleotide multimer comprising multiple copies of the desired RNA oligonucleotide sequence. Preferably, the RNA oligonucleotide multimer is cleaved to produce RNA oligonucleotides having well-defined ends. Preferred RNA oligonucleotide multimers contain ribozymes capable of both cis (autolytic) and trans cleavage.
BioAssay record AID 197831 submitted by ChEMBL: Perfect off-state by site-specific incorporation of o-nitrobenzyl (o-NB) unit into RNase S was determined before photolysis at 10 x E5 M/min.
reaction: see text] Benzyl aryl ether dendrimers containing photosensitive, veratryl-based o-nitrobenzyl AB linkages (bold bonds) were prepared to the third generation and shown to undergo site-specific degradation when irradiated with ultraviolet light ...
Obtain superior separations and LC/MS characterization data for synthetic oligonucleotides, from routine mass and sequence confirmation to a more detailed impurity profile analysis.
Obtain superior separations and LC/MS characterization data for synthetic oligonucleotides, from routine mass and sequence confirmation to a more detailed impurity profile analysis.
Purified Recombinant Human OASL Protein, His-tagged from Creative Biomart. Recombinant Human OASL Protein, His-tagged can be used for research.
The induction of transcription of the 2-5-oligoadenylate (2-5A) synthetase gene by type I (alpha/beta) and type II (gamma) interferons (IFNs) has been studied in wild-type (w.t.) and IFN-resistant Friend leukemia cells (FLC). Following IFN treatment, new complexes are formed in vitro between the IFN-responsive sequence (IRS) of the 2-5A synthetase gene and cellular proteins. Within minutes after IFN-alpha/beta addition to w.t. FLC, an IRS-protein complex, designated F1, is detected, as already observed in several human cell lines. In response to IFN-gamma, a novel complex, designated Fg, is observed in w.t. FLC. The Fg complex appears within 3 h, while an F1-like complex is faintly visible 10 to 24 h later. In the IFN-alpha/beta-resistant FLC, IFN-gamma induces only the Fg complex and fails to induce F1. Fg formation is correlated with the IFN-gamma-induced transcription of the 2-5A synthetase gene and the appearance of the corresponding ...
Oligonucleotides can be synthesized by condensing a protected nucleoside H‐phosphonate monoester with a second nucleoside in the presence of a coupling agent to produce a dinucleoside H‐phosphonate diester
The human 6-16 and ISG12 genes are transcriptionally upregulated in a variety of cell types in response to type I interferon (IFN). The predicted products of these genes are small (12.9 and 11.5 kDa respectively), hydrophobic proteins that share 36% overall amino acid identity. Gene disruption and over-expression studies have so far failed to reveal any biochemical or cellular roles for these proteins. We have used in silico analyses to identify a novel family of genes (the ISG12 gene family) related to both the human 6-16 and ISG12 genes. Each ISG12 family member codes for a small hydrophobic protein containing a conserved ~80 amino-acid motif (the ISG12 motif). So far we have detected 46 family members in 25 organisms, ranging from unicellular eukaryotes to humans. Humans have four ISG12 genes: the 6-16 gene at chromosome 1p35 and three genes (ISG12(a), ISG12(b) and ISG12(c)) clustered at chromosome 14q32. Mice have three family members (ISG12(a), ISG12(b1) and ISG12(b2)) clustered at chromosome 12F1
A 2,5-oligoadenylate (2-5A)-dependent 37-kDa form of RNase L has been reported in extracts of peripheral blood mononuclear cells (PBMC) from individ
BACKGROUND: Inflammation may be involved in the pathogenesis of Alzheimers disease (AD). There has been little success with anti-inflammatory drugs in AD, while the promise of anti-inflammatory treatment is more evident in experimental models. A new anti-inflammatory strategy requires a better understanding of molecular mechanisms. Among the plethora of signaling pathways activated by β-amyloid (Aβ) peptides, the nuclear factor-kappa B (NF-κB) pathway could be an interesting target. In virus-infected cells, double-stranded RNA-dependent protein kinase (PKR) controls the NF-κB signaling pathway. It is well-known that PKR is activated in AD. This led us to study the effect of a specific inhibitor of PKR on the Aβ42-induced inflammatory response in primary mixed murine co-cultures, allowing interactions between neurons, astrocytes and microglia. METHODS: Primary mixed murine co-cultures were prepared in three steps: a primary culture of astrocytes and microglia for 14 days, then a primary ...
BACKGROUND: Inflammation may be involved in the pathogenesis of Alzheimers disease (AD). There has been little success with anti-inflammatory drugs in AD, while the promise of anti-inflammatory treatment is more evident in experimental models. A new anti-inflammatory strategy requires a better understanding of molecular mechanisms. Among the plethora of signaling pathways activated by β-amyloid (Aβ) peptides, the nuclear factor-kappa B (NF-κB) pathway could be an interesting target. In virus-infected cells, double-stranded RNA-dependent protein kinase (PKR) controls the NF-κB signaling pathway. It is well-known that PKR is activated in AD. This led us to study the effect of a specific inhibitor of PKR on the Aβ42-induced inflammatory response in primary mixed murine co-cultures, allowing interactions between neurons, astrocytes and microglia. METHODS: Primary mixed murine co-cultures were prepared in three steps: a primary culture of astrocytes and microglia for 14 days, then a primary culture of
F. Li, P. Pallan, M. Maier, K. Rajeev, S. Mathieu, C. Kreutz, Y. Fan, J. Sanghvi, R. Micura, E. Rozners, M. Manoharan, M. Egli*; Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme; Nucl. Acids Res. 2007, 35, 6424-6438. ...
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
Tytu : Synthesis, physico-chemical and biological properties of DNA and RNA oligonucleotides containing short alkylamino internucleotide bond. ...
Tetherin (BST2/CD317) has been recently recognized as a potent interferon-induced antiviral molecule that inhibits the release of diverse mammalian enveloped virus particles from infected cells. By targeting an immutable structure common to all these viruses, the virion membrane, evasion of this antiviral mechanism has necessitated the development of specific countermeasures that directly inhibit tetherin activity. Here we review our current understanding of the molecular basis of tetherins mode of action, the viral countermeasures that antagonize it, and how virus/tetherin interactions may affect viral transmission and pathogenicity.
Cyclic AMP; 35-cyclic AMP; 6-(6-Amino-9H-purin-9-yl)tetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinine-2,7-diol 2-oxide; Acrasin; Adenosine 3,5-cyclic monophosphate; Adenosine 3,5-cyclic phosphate; Adenosine 3,5-cyclophosphate; Adenosine 3,5-monophosphate; Adenosine 3,5-cyclic monophosphorate; Adenosine 3,5-cyclic monophosphoric acid; Adenosine cyclic monophosphate; Cyclic 3,5-AMP; Cyclic 3,5-adenylate; Cyclic 3,5-adenylic acid; Cyclic adenosine 3,5-phosphate; adenosine cyclic-monophosphate; adenosine-cyclic-phosphate; adenosine-cyclic-phosphoric-acid; ...
Cyclic AMP; 35-cyclic AMP; 6-(6-Amino-9H-purin-9-yl)tetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinine-2,7-diol 2-oxide; Acrasin; Adenosine 3,5-cyclic monophosphate; Adenosine 3,5-cyclic phosphate; Adenosine 3,5-cyclophosphate; Adenosine 3,5-monophosphate; Adenosine 3,5-cyclic monophosphorate; Adenosine 3,5-cyclic monophosphoric acid; Adenosine cyclic monophosphate; Cyclic 3,5-AMP; Cyclic 3,5-adenylate; Cyclic 3,5-adenylic acid; Cyclic adenosine 3,5-phosphate; adenosine cyclic-monophosphate; adenosine-cyclic-phosphate; adenosine-cyclic-phosphoric-acid; ...
Flow cytometry was used to assess peripheral blood mononuclear cells for IL-7-induced proliferation, CD25 expression, and signaling (signal transducer and activator of transcription 5 phosphorylation and Akt phosphorylation) in CD4 T cells. Freshly isolated cells were characterized by expression of IL-7Rα (CD127) among CD4 T-cell maturation subsets by flow cytometry and sorted CD3 T cells were assessed for expression of IFN-α and interferon stimulated genes (2′-5′-oligoadenylate synthetase-1 and myxovirus resistance A protein) by quantitative real-time PCR. Responses to IFN-α were assessed by induction of signal transducer and activator of transcription 1 phosphorylation and inhibition of IL-7-induced CD4 T-cell proliferation.. RESULTS ...
Friday Lectures. Friday, October 20, 2017 3:45 p.m.. Caspary Auditorium. Luciano Marraffini, Ph.D.. Associate Professor and Head. Laboratory of Bacteriology. The Rockefeller University. CRISPR-Cas, Acquired Immunity in Prokaryotes. Recommended Readings:. Niewoehner, Ole; Garcia-Doval, Carmela; Rostol, Jakob T.; et al. (2017). Type III CRISPR-Cas systems produce cyclic oligoadenylate second messengers. NATURE. 548 (7669): 543-+. Modell, Joshua W.; Jiang, Wenyan; Marraffini, Luciano A. (2017). CRISPR-Cas systems exploit viral DNA injection to establish and maintain adaptive immunity. NATURE. 544 (7648): 101-+ Marraffini, Luciano A. (2017). Sensing danger. PNAS. 114 (1): 15-16 Heler, Robert; Wright, Addison V.; Vucelja, Marija; et al. (2017). Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response. MOLECULAR CELL. 65 (1): 168-175. Marraffini, Luciano A. (2015). CRISPR-Cas immunity in prokaryotes. NATURE. 526 (7571): 55-61. McGinn, Jon; ...
Quantified miR-608-target interactions. (A) Target and miRNA RNA oligonucleotides sequences. Seed regions are colored. (B) Predicted structures and binding en
Tres Tintas Barcelona introduces you OAS, the collection designed by Sandberg that becomes a true Oasis. OAS murals generate a timeless feeling, allowing you to rest, both visually and spiritually, as it happens in those fascinating places.. The OAS collection includes 13 murals that combine floral designs, mosaics and textures. Pastel colours and dark midnight shades provide all their dreamy force. The combination evokes a quiet and warm atmosphere, turning any space into a cozy oasis.. The floral wallpapers use shapes such as the leaves of the black elder and introduce shades to create a magic halo. Johanna, Lo and Malin bring nature to the walls, but also an introspective touch that is inspiring and relaxing at the same time.. OAS murals based on patterns are very imaginative and different from each other. You will find from the vintage trompe loeil of Ilse to the volume brush strokes of Stine. There are also classic mosaics as Gaston, textile references as Lene or sober lines as Robin. The ...
Trispiro[furan-2(3H),2-[2H]cyclopenta[b]furan-5(3H),12(2H)-furo[3,2:3,4]cyclopenta[1,2:5,6]naphth[1,2-b]indeno[4,5-i]phenazine-2,2-[2H]pyran]-11(1H)-one,3,3a,3a,4,4,4,4b,5,5,5,6,6,6,6a,6a,7,9,9a,9b,10,12a,13,14,14a,15,17,17a,17b,18,19,19a,19b-dotriacontahydro-4,5,5,19,19b-pentahydroxy-1,3,4,4,5,5,5,9a,17a,19a-decamethyl-,(1S,2S,2R,3S,3aS,3aS,4S,4S,4bR,5R,5S,5S,6aS,6aR,9aS,9bS, ...
All Ive done is take the formula for dAGCT (DNA oligonucleotide) and found the letters corresponding to the quotients (the little numbers) in ascending order. I then translated them. The result said to try the RNA oligonucleotide which is AGCU and add "C of" to it. So I did. The translation that came back with the "C of" added to it is scientifically unexplainable. How could the author have known about DNA and RNA, let alone know how we describe it? He couldnt. So just what is the explanation? Surely it cant be divine intervention again ...
Gene Wilder, star of comedy classics like Young Frankenstein, Blazing Saddles, and Willy Wonka and the Chocolate Factory, died Monday at the age of 83 due to complications from Alzheimers.
This weeks Metropole Paris cartoon. With weekly news, events, features, photos, posters, and a real club for readers who are in Paris. Online since 1996
Researchers have found that short bursts of intense exercise produce similar results to traditional longer-duration workouts, the Mail Online reports…
FavouriteTeamOptions:{MultipleChoice:false,ShowTeams:true,Teams:[{Id:283,Name:1860 Munich},{Id:284,Name:AC Milan},{Id:285,Name:Ajax},{Id:286,Name:AS Monaco},{Id:287,Name:AS Roma},{Id:288,Name:Aston Villa},{Id:289,Name:Athletic Bilbao},{Id:290,Name:Atlanta United},{Id:291,Name:Atletico de Madrid},{Id:292,Name:AZ Alkmaar},{Id:293,Name:Barcelona},{Id:294,Name:Bastia},{Id:295,Name:Bayern Munich},{Id:296,Name:Benfica},{Id:297,Name:Boca Juniors},{Id:298,Name:Bologna},{Id:299,Name:Bordeaux},{Id:300,Name:Borussia Monchengladbach},{Id:301,Name:BVB},{Id:302,Name:Cagliari},{Id:303,Name:Celta Vigo},{Id:304,Name:Celtic},{Id:305,Name:Chelsea},{Id:306,Name:Chicago Fire},{Id:307,Name:Club America},{Id:308,Name:Club Brugge},{Id:309,Name:Colo Colo},{Id:310,Name:Colorado Rapids},{Id:311,Name:Columbus Crew},{Id:312,Name:Cruz ...
The danger of growing resistance to antibiotics should be treated as seriously as the threat of terrorism, Englands chief medical officer says.
ClickBank is the retailer of products on this site. CLICKBANK® is a registered trademark of Click Sales, Inc., a Delaware corporation located at 917 S. Lusk Street, Suite 200, Boise Idaho, 83706, USA and used by permission. ClickBanks role as retailer does not constitute an endorsement, approval or review of these products or any claim, statement or opinion used in promotion of these products.. The websites content and the product for sale is based upon the authors opinion and is provided solely on an AS IS and AS AVAILABLE basis. You should do your own research and confirm the information with other sources when searching for information regarding health issues and always review the information carefully with your professional health care provider before using any of the protocols presented on this website and/or in the product sold here. Neither ClickBank nor the author are engaged in rendering medical or similar professional services or advice via this website or in the product, and ...
Hello.. Welcome to my little part of the world.I love all things English and pretty. I love fabric and stitching, china and roses, Brambly Hedge stories and the sound of childrens laughter. I love all things organic and growing my own vegetables. Cooking and making special gifts. This blog is about my family, friends and my sewing...I am happy for you to come along on this journey with me. Enjoy reading about me and my lifes happenings ...
The report was compiled by a three-member committee last month headed by Ejaz Ahmed Siddiqui, who has since retired, Admin Officer Aftab Saleem and director public relations Mazhar Hussein.. After the inquiry, the administration wing of the authority issued charge-sheets to some 100 employee who were given out of the way promotions, according to member administration, Yasir Pirzada, who had initiated the inquiry.. He added that the Supreme Court has also declared that there is no room for out-of-turn promotions and up-gradations of posts.. "We will uphold the rule of law, come what may," he said.. According to sources, the CDA Employees Union has been backing the cases and that it has been making efforts to stop the issuance of charge-sheets.. The inquiry also cites some examples of up-gradations for instance, an official by the name of Zafar Mahmood was appointed as a mechanic helper in 1982 and that he is at present transport officer in BPS 16 after the post of senior foreman was renamed ...
Becky Carlberg, of Shawnee, Okla., lost most of her garden this year to the recent storms, but said she will make due with whats left - her pumpkins.
Becky Carlberg, of Shawnee, Okla., lost most of her garden this year to the recent storms, but said she will make due with whats left - her pumpkins.
The designations employed and the presentation of information on the FAO website do not imply the expression of any opinion whatsoever on the part of FAO concerning the legal or development status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. The mention of specific companies or products of manufacturers, whether or not these have been patented, does not imply that these have been endorsed or recommended by FAO in preference to others of a similar nature that are not mentioned. Information on this site is provided on an as is and as available basis. FAO makes every effort to ensure, but does not guarantee, the accuracy, completeness or authenticity of the information on the FAO website. FAO reserves the right to alter, limit or discontinue any part of this service at its discretion. Under no circumstances shall FAO be liable for any loss, damage, liability or expense suffered that is claimed to result from the ...
Get the Faya overnight weather forecast for today, tomorrow and 15 days including the overnight low temperature, RealFeel and chance of precipitation for Faya, Saudi Arabia from AccuWeather.com
What am I giving away........ A Millies Market Day Bag pattern by Janelle Wind, 1/2 mt (.45yd) of blue floral fabric, 2 fat quarters 1pink spots, 1 blue allover paisley, 3 skeins of DMC vareigated thread, 1/2 doz vintage wooden pegs, 2mt (1.8yd) of blue ricrac + a few little surprises because I love surprises dont you!. This bundle of goodies would love to come to your house!. How to enter?. 1 entry : Leave a comment on my blog - if you do not have a blog you MUST leave your email address or I cannot contact you and your entry will not be counted.. 1 entry : Put the Gum Tree Designers button on your blog sidebar and link it back to Gum Tree Designers(you can copy the button from my previous post - just alternate click on it and save image as.." to your computer). 1 entry : Become my follower and a follower of Among the Gum Trees!. Thats 3 entries if you do all that Ive asked. Please leave ONE comment for EACH entry...so if you are eligible for 3 entries leave 3 comments. If you are ...
Now oi asks meself: who ere as thee power to set up sech a filter for their own hamyoosement hmmm? An who ere as hadmitted tsetting sech filters in thee recent past? An who ere moight be gettin a bit o theyre own backe ...
This website is provided by EMD Serono, a business of Merck KGaA, Darmstadt, Germany. This website is not intended for residents of the United States.. The information contained on this website is provided exclusively for general information purposes. Whilst we have taken reasonable care in compiling this website, we are not responsible for any action taken by any person or organization, wherever they are based, as a result of, direct or otherwise, information contained in or accessed through this website, whether such information is provided by us or by a third party. Nothing on this website should be construed as the giving of specific medical advice or the making of any recommendation. This website should not be relied upon as the basis for any medical decision or action.. As a result of ongoing medical advances and developments, the information on this website may not always be completely up to date and, for this reason, such information is provided on an as is and as available basis. We ...
Get the Harfayn weather forecast. Access hourly, 10 day and 15 day forecasts along with up to the minute reports and videos for Harfayn, Saudi Arabia from AccuWeather.com
Ergonomically designed for all body types, features adjustable height Isometric non-stick grips Personal workout timer starts and stops when you do Compact folding design allows for easy storage
Exercise may be just as good as medication to treat heart disease and should be included as a comparison when new drugs are being developed and tested, scientists say.
Resveratrol exhibits a strong cytotoxic activity in cultured cells and has an antiviral action against polyomavirus: potential clinical use. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Farrell, P J.; Broeze, R J.; and Lengyel, P, "Accumulation of an mrna and protein in interferon-treated ehrlich ascites tumour cells." (1979). Subject Strain Bibliography 1979. 137 ...
Asparagine synthetase antibody (asparagine synthetase (glutamine-hydrolyzing)) for IHC-P, WB. Anti-Asparagine synthetase pAb (GTX114269) is tested in Human samples. 100% Ab-Assurance.
return { 1322438400:270, 1322524800:270, 1322611200:283, 1322784000:329, 1322870400:283, 1322956800:346, 1323043200:383, 1323129600:403, 1323216000:424, 1323302400:446, 1323388800:469, 1323475200:469, 1323561600:493, 1323648000:544, 1323734400:572, 1323820800:572, 1323907200:600, 1323993600:630, 1324080000:630, 1324166400:662, 1324252800:731, 1324339200:768, 1324425600:807, 1324512000:848, 1324598400:848, 1324684800:891, 1324771200:936, 1324857600:936, 1324944000:978, 1325030400:1016, 1325116800:1016, 1325203200:1046, 1325289600:1084, 1325376000:1084, 1325462400:1113, 1325548800:1113, 1325635200:1151, 1325721600:1122, 1325808000:1071, 1325894400:1071, 1325980800:1018, 1326067200:991, 1326153600:975, 1326240000:975, 1326326400:939, 1326412800:939, 1326499200:916, 1326585600:870, 1326672000:831, 1326758400:818, 1326844800:811, 1326931200:811, 1327017600:801, 1327104000:793, 1327190400:825, ...
I believe that the Offices of the General Secretariat in the OAS Member States perform an important role. In many countries these National Offices do not only have representational functions, their presence is also politically important, while they also contribute to capacity building in particularly the small states. I believe that in the context of creating a more efficient and effective Organization we have to analyze the role and scope of work of these entities. New functions can be added to their portfolio making them more useful in carrying out the tasks assigned to the OAS ...
Oas1g - mouse gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN311406G1|/strong|, Oas1g gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.
Read reviews, compare customer ratings, see screenshots and learn more about OAS Mobile App. Download OAS Mobile App and enjoy it on your iPhone, iPad and iPod touch.
This requires a response. No, I for one will not call Jeff a racist at all. However, just by your name I have always assumed you had those inclinations.. I sure am glad you admitted what you are, and I could not have said it better myself. -A hack, along with uninformed, ignorant and a Colon Hater. Still doing the name calling, That is the definition of a hater. Jeff is none of those. While I disagree with his assessment, I think he has been one of the best QBs after Joe and Steve.. To some of the ignorant and uninformed, I will try to set the record straight. Jeff Garcia said some pretty harsh things about Kaep, I will not elaborate, but it is all in the archives. For that reason, Kaep chose Warner over Jeff. Jeff cannot burn bridges and expect different treatment. Jeff got so desperate, he was begging in a wheedling tone, but it was too late. Many wondered why Kaep chose Warner over Jeff. Kaep explained it as not needing the mobility coaching, he wanted to work on his weaknesses, and Warner ...
t· RESERVE 1964 ilflllliiil . 50004759 ~ ............ -A.~.... Report l ? 1964 Southwest Foundation for Research and Education ...
head,,base target=_blank /,,/head,,html, ,head,,meta name=verify-v1 content=AS31njSF6+TSNOdeXwxfLUr19vHgBpQTL3sNZ6c0ucM= , ,meta name=verify-v1 content=OkEQEnnWGiNoIVREc4jufRx+5eNW0ESeedrMCsvY22o= , ,meta name=verify-v1 content=5fdEb3xnXHYkPLnBiORLlsIOWF1ukH3rnFcJWI3xa1w= /, ,style type=text/css, .pg-weather { display: none !important; } ,/style,,/head, ,body,,center, ,ul style=display:inline; list-style-type:none, ,li style=display:inline,,a style=padding:0 10px;font-size: 12px; target=_parent href=https://triangle411.partners.local.com,Add or Enhance Your Listing,/a,,/li, ,li style=display:inline,,a style=padding:0 10px;font-size: 12px; target=_parent href=http://www.wral.com/apps/feedback/feedback/?d_id_person=413,Contact Us,/a,,/li, ,li style=display:inline,,a style=padding:0 10px;font-size: 12px; target=_parent href=http://www.wral.com/apps/feedback/feedback/?d_id_person=413,Raleigh, NC News,/a,,/li, ,li style=display:inline,,/li,,li style=display:inline,,a ...
h -r J, : Ilf r fu . wf: : : ::; : ,:,:,:;:; ;:: ; \ ;:; ru: : ,/H.:;; ::: I ::Y: .. ,..: ,:: : : i(:;:,: :,: u { :t! : . t !H tu: ;iL:Â I- :::V; tt i \ r , i .. l! t 11 ...t; ,/C ,::::, ;;.,, Mi: . J -lIV ::,,,,,;.:.:,::::,::::::::::;,,,. w :.. ..,.:,:-,::::: W:.. :: : :::y: :,(: , :::: , J,;: : ::; } ,: ::: :: ::: y } - .fi,;;{- /I ,:f ;, L .):,fE\:,::,:.:: , ,:,,..:il:,:::::,: ,aiJt ,,:,:,,: 11\, æ{. ,ÆF! :,:t:, \::}u: 1\ u, ;:::.. :::::::t ,:,: i2 ; j;:1t;f::::: ::l ] , I 1 - - -- - I .. ... %,, XN:Y(;. : ,,,^ ^ g z. , J ?; .....}f l , :::, I: ;: -:.;.: ...;-.-:::. 22 4 la ! : :, : : :. : , : : :: . : . ; , I:,: ., .. I :,; l l i: ,:. .:.?: :::: - .... , 1: j} f ):: !--&, , -::=.:- .llt ::-- :-:. .--.- ....MI. L : ,\,li lj ... , - ( r :=h :: . :t ;; :::: p;.:: :::: 1 ;,i;; ::J;\?: ) :- :.::. :;. : . :::{ , ;: ,\: [email protected] ,: .:,.. ;: ...
Glutamine Synthetase兔多克隆抗体(ab49873)可与小鼠, 大鼠, 猴样本反应并经WB, IHC,ICC/IF实验严格验证,被8篇文献引用并得到8个独立的用户反馈。
Glutamine Synthetase兔多克隆抗体(ab73593)可与小鼠, 大鼠, 狗, 人, 狨猴样本反应并经WB, IHC, ICC/IF实验严格验证,被3篇文献引用并得到8个独立的用户反馈。
He added that RSS could be used as some type of time-shift notification, where one shift manager updates a status report so the next employee can see what happened. Coté is one of a few people who have written about the possibility, mainly in blogs. Fred Johanneson, BMC Software vice president and program executive for datacentre solutions, is another. BMC doesnt currently have the RSS feature within its systems management software, nor do other major suppliers, such as IBM, Hewlett-Packard and CA. But Johanneson is interested. He said RSS could be used as an infrastructure communicator between programs. As changes and configurations happen within that infrastructure that could affect that business, RSS could be a tool on what is affected, he said. Programs or people can decide how they want to respond to that. RSS in play today One company that has integrated RSS into its systems management software is Hyperic. CEO Javier Soltero said Hyperic has had RSS in its systems management ...
141. went to the commanding officer and told him I had a feeling that I was going to get it this time, and that I couldnt take it any more. He gave me a direct order to, and it was either do that or have a bad record, so I went. This soldier had tried on three occasions to have his rank reduced to that of private. You see, he said, as a platoon sergeant, you are more often than not a platoon leader, and I couldnt lead the men like I did before. Under shelling I got jittery. A platoon sergeant is a leader. If he isnt out in front it affects the men.. This soldier was born on a ranch in Texas. He stated that his father was epileptic, but that he rarely worried about it and that it had not affected him in any way. His parents were harmoniously married. There were seven children, of which he was next to the oldest. He had a happy family life and had many friends on nearby ranches. No significant neurotic traits or conflicts were elicited in the history. Enuresis, nail biting, temper ...
The second important thing is Keiths talent. We took it for granted, in a way, as he says. We felt it was our duty to get together and write a song, one good song each day we worked. He is kind to say I could take what he gave me and run with it. But he is the one who gave me the actual song to write the lyrics to. He wrote a dozen Top 10 hits in five years, and, after the band added Mick Taylor and essentially grew up, he wrote most of Beggars Banquet and Let It Bleed. Again: What were you doing at 25? Its interesting to me how no previous song wed recorded would have a respectable place on those albums; and any song on them would have seem out of place even on Aftermath or Between the Buttons. Keiths lurch forward was amazing. As a pure rock (not folk or pop) songwriter, I think he is not just without peer. I think he is unrivaled in depth and growth, from As Tears Go By to Satisfaction to Jumping Jack Flash to, I dont know, Gimme Shelter. Monkey Man. Street Fighting Man. The ...
My mom was telling me about one of my relatives whos been having blood problems for a few years. Now, she said, his red cell count is way down.Thats not good, I said. Guess hed better eat his liver.I thought for a second, then added, Well, not his own liver...As I reflected on the conversation…
By Clarence E. Hill Jr.. Oxnard, Calif. _ So now we know why Cowboys owner Jerry Jones, the original Mr. Socks to Jocks himself, has yet to speak with receiver Dez Bryant about the misdemeanor family violence charge for allegedly assaulting his mother.. Asked on his radio show on 105.3 the fan Tuesday morning to explain why he hasnt talked to Bryant about the incident which could potentially take him off the field under the NFLs personal conduct polcy if he is found guilty, Jones was straight and to the point.. "Because Im too pissed to talk to him," Jones said.. The Cowboys owner expanded on his feelings when he talked to reporters after practice on Tuesday.. One of the reasons is I didnt want to talk to him emotionally, Jones said. As a daddy sometimes, youre not supposed to spank when youre mad. Like I said the other day, I wanted to get my information, but I also wanted to get my mind right so we could have a good talk about things.. Jones did acknowledge that has now talked to ...
NEW YORK - A few months after announcing a test run of its reduced-sugar cola in Iowa and Wisconsin, PepsiCo unveiled plans for a nationwide rollout of Pepsi Next.. According to published reports, Pepsi Next is expected to hit store shelves in spring 2012.. As part of our continued commitment to innovate our carbonated soft drinks portfolio, we developed Pepsi Next, a great-tasting, full-flavor cola with 60% less sugar, PepsiCo Beverages America CEO Massimo dAmore said of the new beverage over the summer. In thorough consumer research, Pepsi Next resonated very well with consumers who are seeking a new, reduced-sugar alternative to their loved cola.. ...
There needs to be responsibility for actions, Adams said. As someone who is pro-life, this is also an attempt and a hope to keep the two people who have created that child together, and I suppose if you just go back to the simple beginning, there is merit to chastity, and to young men and women waiting until marriage. ...
I was waiting to go into a club recently (well, actually waiting for my friends to make up their minds about going in) and a bunch of people walked past us, one of whom yelled I love my life, to which we all responded as a DICKHEAD!. Then a girl in the group sang back all my friends are dickheads and I responded you said it, love ...
Helfant Hopes All-Star Event Will Reach Casual Fans ATP World Tour Exec Chair & President Adam Helfant will introduce an All-Star showcase next year as part of his efforts to help boost the sports popularity, according to Howard Fendrich of the AP. Helfant: Were looking
You got to starve the pigs for a few days, then the sight of a chopped-up body will look like curry to a pisshead. You gotta shave the heads of your victims, and pull the teeth out for the sake of the piggies digestion. You could do this afterwards, of course, but you dont want to go sievin through pig shit, now do you? They will go through bone like butter. You need at least sixteen pigs to finish the job in one sitting, so be wary of any man who keeps a pig farm. They will go through a body that weighs 200 pounds in about eight minutes. That means that a single pig can consume two pounds of uncooked flesh every minute. Hence the expression, as greedy as a pig. ...
2. Now that youre expecting, your couple issues will go away. Quite the contrary, whatever couple issues are present often become inflamed during pregnancy. Even if youre both distracted from the couple problems now, this is the time to get help. Dont wait, since it will become even more stressful when the baby comes and youll have less time and energy to deal with them then.. 3. All that should matter is that your baby is healthy. If youre experiencing gender disappointment, for instance, you might be hearing others around you say, As long as the babys healthy, thats all that matters. Youre allowed to feel disappointment and youll move through it. In good time after you meet your baby, youll be fine with the gender. You might also be disappointed with aspects of your labor and delivery or other topics, so make sure you talk these through with a wonderful nonjudgmental support person.. 4. You should be bonding with your baby already. Bonding is a process of getting to know each other ...
The only characteristic that frozen cells share with this definition is the one of organization. For all intents and purposes, a cryogenically frozen organism is dead and lifeless. Navarick says As the cell is thawed, the pre-existing property of life enables biochemical activity to resume.. The enabler is not the pre-existing quality of life but the thawing of the organism. Navaricks cryogenic argument actually bolsters the contention that life is not some unperceptable consequence of a god but rather that life is a product of, and is equivalent to, biochemical activity ...
TOKYO--Three Bank of Japan policy board members expressed dissent to parts of the central banks semiannual outlook report issued in October, minutes of the boards Oct. 31 board meeting showed on Tuesday, with two of them arguing that the central bank should highlight the downside risks the economy faces.. When the BOJ released the latest report a little less than a month ago, Gov. Haruhiko Kuroda disclosed at a press conference that three members --Sayuri Shirai, Takehiro Sato and Takahide Kiuchi--were opposed to parts of the report.. The minutes shows that Ms. Shirai proposed to add the phase that attention should be paid to downside risks since there is a high degree of uncertainty over foreign economies as well as jobs and income conditions at home.. While most members endorsed an expression that Japans economy has been following a path toward attaining its 2.0% inflation target as expected, she also questioned the use of the word as expected, proposing to change it to at a moderate ...
I dont normally “web log†my feelings but Im actually pretty nervous about this whole CD release. I was all “beh whatever dudes well see how it goes, just another day on the grind you know dog†until a couple hours ago when I became like !!!! HOLY LORD. It feels like my future as an artist sort of hinges on it but then I feel that way about everything.. Honestly, I think the neurotic anxiety has been getting to me for a couple weeks but Ive had tasks to keep me busy. Two weeks ago I was sleeping away peacefully and then I guess I tore at my forehead with my retarded talons. As you can see from the picture up there I managed to put a two inch scratch in my dome. That aint relaxed behavior. The CD release party is tomorrow night. Its sort of a drag though, you folks who read my blog and have been with me for a long time are not generally in my town so you wont be there. So that part breaks my heart kinda because I love you guys okay now the vodkas talking catch you ...
Lorraine Pombert once flew in Army airplanes that would sneak into war-ravaged regions of Europe to pick up wounded soldiers at night.As long as a man had a pulse, we would take him, said Pombert,
thparker writes As part of the Media Center release discussed previously, Bill Gates had an interview with USA Today. Best quote: Q: Speaking of security, Internet Explorer has had well-publicized holes... Gates: Understand those are cases where you are downloading third-party software. Well no...
The role that education plays in issues of social equity and justice cannot be undervalued. It is acknowledged by the United Nations as a human right, Everyone has the right to education (United Nations, 1948) and as outlined in the Melbourne Declaration on the Educational Goals for Young Australians As a nation Australia values the central role of education in building a democratic, equitable and just society- a society that is prosperous, cohesive and culturally diverse, and that values Australias Indigenous cultures as a key part of the nations history, present and future. (Barr et al, 2008). Such lofty assertions of the importance of education as a right and national value should be sufficient to ensure that all Australians have access to an education of the highest standard with equitable outcomes for all, the reality is that this is not the case ...
You can even see the a beating heart brain and other organs because the scales and skin have no pigments Yutaka Tamaru said, an associate professor at Mie University. As this goldfish grows bigger, you can watch its whole life, Tamaru said. ...
As long as Im director of CIA, irrespective of what the president says, Im not going to be the director of CIA that gives that order, he said.
Last week, Old Pueblo Distribution released the second title from the critically acclaimed director Uncle Lanny. Within the first 5 days of release, Uncle Lannys As Real as it Gets #1 exceeded sales of his first title Uncle Lanny Loves College Co-eds. Uncle Lannys first release did extremely well for a first title.
As I hurdled through space, one thought keep crossing my mind-every part of this rocket was supplied by the lowest bidder. John Glenn When the white missionaries came to Africa,...
As far as taxation is concerned, we have asked for a clear roadmap on the 25 per cent (corporate tax)... We are totally in support of removal of incentives and allowances, CII President Sumit Mazumder said.
Read 4 responses to: As a mother, I am constantly at this dilema when it... Find the best answer on Mamapedia - mom trusted since 2006.
The Memphis Grizzlies confirmed Friday that rookie Jaren Jackson Jr. will be out indefinitely with a deep thigh bruise. According to a team release, updates will be provided as appropriate. The 
Book now at Chicago Cut Steakhouse in Chicago, explore menu, see photos and read 5154 reviews: As always a great meal. Nice wine list and wonderful service in both the bar and restaurant. We will be back!
As Catalonia goes to the polls, Sid Lowe looks at one of the regions great cultural sporting icons and its role in Catalan identity... | Geography Education
As more HIV-positive people live longer in an era of widespread use of ART, services will need to be adapted for this populations changing needs, ...
Like you, I become interested in medical astrology from time to time on an as needs basis. Unless you are a full-time astrologer, the strict rules of decumbiture provide examples only occasionally. I tend to rely on times provided by friends of mine having a medical crisis. This isnt a very systematic way of studying decumbiture, but over the past few months several of my close friends have experienced illnesses resulting in need for bed rest, ER visits, etc. So I will try to get a few more permissions to post decumbiture charts from these folks for discussion here ...
Quotes are not sourced from all markets and may be delayed up to 20 minutes. Information is provided as is and solely for informational purposes, not for trading purposes or advice.Disclaimer ...
Christian thrash metallers TOURNIQUET have been rejoined by longtime friend, guitarist Aaron Guerra. As you may remember, it was a mutual parting ...
Quotes are not sourced from all markets and may be delayed up to 20 minutes. Information is provided as is and solely for informational purposes, not for trading purposes or advice.Disclaimer ...
Quote of the Week (From Organic Bytes) Monsantos Corn Is Toppling Over As the summer growing season draws to a close, 2011 is emerging as the year of the superinsect - the year pests officially developed resistance to Monsantos genetically engineered (ostensibly) bug-killing corn. In late July scientists in Iowa documented the existence of corn…
LOUISVILLE, KY (WAVE) - Try It Before You Buy It is a new segment well be doing on WAVE 3 Sunrise and WAVE 3 News at 5:30pm every Friday. Ill be testing those As Seen on TV products that promise
See also: *An Englishman in BBQ Sauce for more of our efforts to fatten Gavin up Last week my dad emailed me. As a parent the sheer amount of food you...
mr_sifter writes As previously discussed, computers running [email protected] now contribute over 1 petaflop of processing power to research into protein folding, making [email protected] the most successful example yet of a distributed computing app. Its also at the forefront of GPGPU computing, with both ...
Oxhoof, OXIDE, Oxigena, Oyster Node, Oyster Node, oystereye, OZ, OZ, Ozawa, ozengué, P&Oetry, P.i.l%s, P.M.E, P.M.P.…
Recombinant Human 2-5-Oligoadenylate Synthetase 1, 40/46kDa, His-tagged OAS1-1399H - Creative BioMart  Recombinant Human 2'-5'-Oligoadenylate Synthetase 1, 40/46kDa, His-tagged OAS1-1399H - Creative BioMart
Oligoadenylate Synthetase 1, 40/46kDa, His-tagged. Download Datasheet See All OAS1 Products. Bring this labeled protein ... oligoadenylate synthetase 1; (2-5")oligo(A) synthetase 1; 2-5A synthetase 1; p46/p42 OAS; E18/E16; OAS1; OIAS; IFI-4; OIASI; 2- ... oligoadenylate synthetase 1, 40/46kDa; 2"-5" oligoadenylate synthetase 1 p48 isoform; 2"-5" oligoadenylate synthetase 1 p52 ... OAS1 enzyme is a member of the 2μ,5μ-oligoadenylate synthetase family. OAS1 is induced by interferons and uses adenosine ...
more infohttps://www.creativebiomart.net/description_20759_8.htm
Enzymatic activity of 2-5-oligoadenylate synthetase is impaired by specific mutations that affect oligomerization of the...  Enzymatic activity of 2'-5'-oligoadenylate synthetase is impaired by specific mutations that affect oligomerization of the...
... synthetase causes a loss of its enzyme activity (Ghosh, S. K., Kusari, J., Bandyopadhyay, S. K., Samanta, H., Kumar, R., and ... synthetases revealed that the residues at positions 330 to 333 are highly conserved. Alanine-scanning mutagenesis of these ... Previous studies from our laboratory have shown that deletion of residues 321 to 344 of the 9-2 isozyme of 2-5-oligoadenylate ... These results indicate that tetramerization of the protein is required for the enzymatic activity of the small 2-5(A) ...
more infohttps://www.semanticscholar.org/paper/Enzymatic-activity-of-2-5-oligoadenylate-synthetas-Ghosh-Sarkar/7490dfad89b648f7b1457b0a927d58cffdab16cd
Inhibition of Double-Stranded RNA-Dependent Protein Kinase PKR by Vaccinia Virus E3: Role of Complex Formation and the E3 N...  Inhibition of Double-Stranded RNA-Dependent Protein Kinase PKR by Vaccinia Virus E3: Role of Complex Formation and the E3 N...
... oligoadenylate synthetase/RNase L portion of the interferon response pathway (2).. The C-terminal portion of E3 containing the ... 2A and B). To verify that this mutant protein was incapable of binding dsRNA, we compared it to wild-type E3 for the ability to ... 2A and B), whereas Ala substitutions at Phe-67 and Met-68, flanking Trp-66, had no effect on E3 function (Fig. 2A). It was ... 2 to 4 indicate that both the dsRNA binding activity of E3 and residues in its N-terminal domain between amino acids 60 and 82 ...
more infohttps://mcb.asm.org/content/18/12/7304?ijkey=d06698f2e38f05920198005f523aef1080b69713&keytype2=tf_ipsecsha
ETIS - Reduction of 2-5-oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis  ETIS - Reduction of 2'-5'-oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis
... oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis. Kirje Kelve, M.; Truve, E.; Aaspõllu ... oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis. Müller, W. E. G.. Use of Aquatic ... oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis ... A.; Kuusksalu, A.; Dapper, J.; Perovic, S.; Müller, W. E. G. (1994). Reduction of 2-5- ...
more infohttps://www.etis.ee/Portal/Publications/Display/0450a787-2759-4a43-bed1-dcdd6899a8cd
Establishment of a nonradioactive assay for 2-5 oligoadenylate synthetase and its application in chronic hepatitis C patients...  Establishment of a nonradioactive assay for 2'-5' oligoadenylate synthetase and its application in chronic hepatitis C patients...
... oligoadenylate synthetase (2-5 AS) and to measure the 2-5AS in peripheral blood mononuclear cell (PBMC) extracts of patients ... oligoadenylate synthetase (2-5 AS) and to measure the 2-5AS in peripheral blood mononuclear cell (PBMC) extracts of patients ... oligoadenylate synthetase and its application in chronic hepatitis C patients receiving interferon-alpha. ... The 2-5AS activity of PBMC in normal persons was in a quite low level (2.0%), and it was increased about ten-folds after ...
more infohttp://www.biomedsearch.com/nih/Establishment-nonradioactive-assay-2-5/11819237.html
Porcine 2′, 5′-Oligoadenylate Synthetases Inhibit Japanese Encephalitis Virus Replication In Vitro  Porcine 2′, 5′-Oligoadenylate Synthetases Inhibit Japanese Encephalitis Virus Replication In Vitro
... oligoadenylate synthetases (OAS) are antiviral proteins and several isoforms have been identified as flavivirus-resistance ... Porcine 2′, 5′-Oligoadenylate Synthetases Inhibit Japanese Encephalitis Virus Replication In Vitro. ...
more infohttps://insights.ovid.com/jomv/201605000/01445456-201605000-00004
Lymphocytic 2,5-oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and...  Lymphocytic 2',5'-oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and...
... oligoadenylate synthetase (2,5AS) activity, determined in homogenates of peripheral B and T lymphocytes. From cellular ATP, ... oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and immunoglobulin M and ... oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and immunoglobulin M and ... oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and immunoglobulin M and ...
more infohttps://cvi.asm.org/content/2/3/302
Nucleotide Oligomerization Domain-2 Interacts with 2-5- Oligoadenylate Synthetase Type 2 and Enhances RNase-L Function in THP...  Nucleotide Oligomerization Domain-2 Interacts with 2'-5'- Oligoadenylate Synthetase Type 2 and Enhances RNase-L Function in THP...
oligoadenylate synthetases. OAS2. 2-5- oligoadenylate synthetase type 2. poly(I:C). polyinosinic acid : polycytidylic acid. ... Oligoadenylate Synthetase Type 2 and Enhances RNase-L Function in THP-1 Cells. Jae W. Dugan,1,2,# Amador Albor,1,2,# Larry ... oligoadenylate synthetase by the herpes simplex virus type 1 Us11 protein. J Virol. 2007;81:3455-3464. [PMC free article] [ ... oligoadenylate synthetase. J Biol Chem. 1999;274:1848-1855. [PubMed]. *Silverman RH, Skehel JJ, James TC, Wreschner DH, Kerr IM ...
more infohttp://pubmedcentralcanada.ca/pmcc/articles/PMC2787966/
Activity of 2,5-oligoadenylate synthetase and interferon level in lymphocytes of rats exposed to radiation - Open Access...  Activity of 2',5'-oligoadenylate synthetase and interferon level in lymphocytes of rats exposed to radiation - Open Access...
... oligoadenylate synthetase (2,5-OAS) activity and interferon (IFN) level in lymphocytes of rats irradiated with 0.25, 0.5, ... Murine Pancreatic Beta TC3 Cells Show Greater -Oligoadenylate Synthetase ( AS) Antiviral Enzyme Activity and Apoptosis ... oligoadenylate synthetase (2,5-OAS) activity and interferon (IFN) level in lymphocytes of rats irradiated with 0.25, 0.5, ... Oligoadenylate Synthetase by Stem Loops at the 5′-End of the West Nile Virus Genome. ...
more infohttp://www.oalib.com/paper/2727277
A specific isozyme of 2-5 oligoadenylate synthetase is a dual function proapoptotic protein of the Bcl-2 family. - Semantic...  A specific isozyme of 2'-5' oligoadenylate synthetase is a dual function proapoptotic protein of the Bcl-2 family. - Semantic...
... linked oligoadenylates that activate RNase L and cause mRNA degradation. Because they all can synthesize 2-5(A), the reason for ... synthetase has an additional activity: it promotes apoptosis in mammalian cells. The proapoptotic activity of 9-2 was isozyme- ... synthetases are a family of interferon-induced enzymes that polymerize ATP into 2-5 ... The 9-2-expressing cells exhibited many properties of cells undergoing apoptosis, such as DNA fragmentation, caspase activation ...
more infohttps://www.semanticscholar.org/paper/A-specific-isozyme-of-2%27-5%27-oligoadenylate-is-a-of-Ghosh-Sarkar/8971af90b1202b7b7349200ff4737116bde316e5
2,5-Oligoadenylate Synthetase 2 (OAS2) Polyclonal Antibody | Technique alternative | 01012487543 - Bioxys  2',5'-Oligoadenylate Synthetase 2 (OAS2) Polyclonal Antibody | Technique alternative | 01012487543 - Bioxys
Oligoadenylate Synthetase 2 OAS2 Polyclonal Antibody 01012487543 at Gentaur 2,5 Oligoadenylate Synthetase 2 (OAS2) ... Oligoadenylate Synthetase 2 (OAS2) Polyclonal Antibody. View details of this awesome product ... If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5° ... 2,3-Cyclic Nucleotide 3-Phosphodiesterase (CNP) Polyclonal Antibody. See more details ...
more infohttps://bioxys.com/catalog/1-bioma/105645-2-5-oligoadenylate-synthetase-2-oas2-polyclonal-antibody-technique-alternative-01012487543
Regulation of 2,5-oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor<...  Regulation of 2',5'-oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor<...
... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor. Molecular and Cellular Biology, 9( ... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor. Mariano Garcia-Blanco, P. Lengyel ... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor, Molecular and Cellular Biology, ... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor. In: Molecular and Cellular Biology ...
more infohttps://researchexperts.utmb.edu/en/publications/regulation-of-25-oligoadenylate-synthetase-gene-expression-by-int
Dadun: IFN-alpha5 mediates stronger Tyk2-stat-dependent activation and higher expression of 2,5-oligoadenylate synthetase...  Dadun: IFN-alpha5 mediates stronger Tyk2-stat-dependent activation and higher expression of 2',5'-oligoadenylate synthetase...
... oligoadenylate synthetase were higher in cell samples treated with IFN-alpha5 than with IFN-alpha2. These findings suggest that ... oligoadenylate synthetase than IFN-alpha2 in liver cells. J Interferon Cytokine Res 2004 Aug;24(8):497-503. ... Oligoadenylate synthetase/metabolism. DNA-Binding proteins/metabolism. Hepatocytes/drug effects. Interferon-alpha/pharmacology ... IFN-alpha5 mediates stronger Tyk2-stat-dependent activation and higher expression of 2,5-oligoadenylate synthetase than IFN- ...
more infohttp://dadun.unav.edu/handle/10171/23014
Oas2 - 2-5-oligoadenylate synthase 2 - Mus musculus (Mouse) - Oas2 gene & protein  Oas2 - 2'-5'-oligoadenylate synthase 2 - Mus musculus (Mouse) - Oas2 gene & protein
... oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its ... oligoadenylate synthetase activity Source: MGI ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the ... oligoadenylate synthetase gene family.". Kakuta S., Shibata S., Iwakura Y.. J. Interferon Cytokine Res. 22:981-993(2002) [ ... oligoadenylate synthetase gene family.". Kakuta S., Shibata S., Iwakura Y.. J. Interferon Cytokine Res. 22:981-993(2002) [ ...
more infohttp://www.uniprot.org/uniprot/E9Q9A9
Recombinant Human OAS1 protein (ab116476) | Abcam  Recombinant Human OAS1 protein (ab116476) | Abcam
25 oligo A synthetase 1. *25 oligoadenylate synthetase 1. *25 oligoisoadenylate synthetase 1 ...
more infohttp://www.abcam.com/recombinant-human-oas1-protein-ab116476.html
2-5-oligoadenylate synthase - Wikipedia  2'-5'-oligoadenylate synthase - Wikipedia
... oligoadenylate synthetase is an enzyme. It is an antiviral enzyme that counteracts viral attack by degrading viral and host RNA ... oligoadenylate synthetase, also referred to as domain 2 of the enzyme, is largely alpha-helical and homologous to a tandem ... oligoadenylate synthetase". Mol. Cell. 12 (5): 1173-85. doi:10.1016/S1097-2765(03)00433-7. PMID 14636576. This article ... oligoadenylate synthetases. Structure-function relationships". J. Biol. Chem. 266 (23): 15293-9. PMID 1651324. Hartmann R, ...
more infohttps://en.wikipedia.org/wiki/2'-5'-oligoadenylate_synthase
The Ebola virus VP35 protein functions as a type I IFN antagonist | PNAS  The Ebola virus VP35 protein functions as a type I IFN antagonist | PNAS
... oligoadenylate synthetase;. ISRE,. IFN-stimulated response element;. GP,. glycoprotein;. pfu,. plaque-forming units;. moi,. ... oligoadenylate synthetase (OAS), and the Mx proteins (7-10).. Many viruses have evolved mechanisms to subvert the host IFN ... 1994) in Encyclopedia of Virology, eds Webster R G, Granoff A (Academic, New York), 2, pp 827-831. ... 5). Further, coexpression of VP35 and NP was able to block ISRE activation to the same extent as expression of VP35 alone (Fig ...
more infohttps://www.pnas.org/content/97/22/12289?ijkey=798cf74f9d659e5dc6894f776932b1d3e5db2119&keytype2=tf_ipsecsha
Oasl2 - Wikipedia  Oasl2 - Wikipedia
... oligoadenylate synthetase-like 2 is a protein that in the house mouse is encoded by the Oasl2 gene. The gene is also known as ... oligoadenylate synthetase with a ubiquitin-like extension, is induced during maturation of murine dendritic cells". Journal of ... oligoadenylate synthetase-like 2". Retrieved 2011-12-11. "Salmonella infection data for Oasl2". Wellcome Trust Sanger Institute ... oligoadenylate synthetase gene family". Molecular Biology and Evolution. 17 (5): 738-750. doi:10.1093/oxfordjournals.molbev. ...
more infohttps://en.wikipedia.org/wiki/Oasl2
Recombinant Human OASL Protein, His-tagged OASL-310H - Creative BioMart  Recombinant Human OASL Protein, His-tagged OASL-310H - Creative BioMart
OASL; 2-5-oligoadenylate synthetase-like; 59 kDa 2-5-oligoadenylate synthase-like protein; p59OASL; TRIP14; TRIP-14; p59 OASL; ... Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.. ... The loss rate is less than 5% within the expiration date under appropriate storage condition.. ... TR-interacting protein 14; thyroid receptor-interacting protein 14; 59 kDa 2-5-oligoadenylate synthetase-like protein;. ...
more infohttps://www.creativebiomart.net/recombinant-human-oasl-protein-his-tagged-502079.htm
KEGG ENZYME: 2.7.7.84  KEGG ENZYME: 2.7.7.84
Oligoadenylates of chain lengths 2, 4 and 5 are also produced. The dimer does not have any known biological activity [2].. ... 5oligoadenylate synthetase family: unique interferon-inducible enzymes catalyzing 2-5 instead of 3-5 phosphodiester bond ... Chemical substance (4) KEGG COMPOUND (4) Chemical reaction (3) KEGG REACTION (2) KEGG RCLASS (1) Gene (225) KEGG ORTHOLOGY (1) ... 2) EGENES (11) Protein sequence (54) UniProt (13) SWISS-PROT (13) RefSeq(pep) (22) PDBSTR (6) DNA sequence (24) RefSeq(nuc) (24 ...
more infohttp://www.genome.jp/dbget-bin/www_bget?ec:2.7.7.84
  • The human double-stranded RNA (dsRNA)-dependent protein kinase PKR inhibits protein synthesis by phosphorylating translation initiation factor 2α (eIF2α). (asm.org)
  • PKR interferes with virus propagation by phosphorylating the α subunit of translation initiation factor 2 (eIF2α), converting eIF2 from a substrate to an inhibitor of its guanine nucleotide exchange factor, eIF2B. (asm.org)
  • The dimer does not have any known biological activity [2]. (genome.jp)
  • To determine 2',5'-oligoadenylate synthetase (2',5'-OAS) activity and interferon (IFN) level in lymphocytes of rats irradiated with 0.25, 0.5, 0.75 and 1.0 Gy doses, as well as to study the effect of interferon inducers on these parameters. (oalib.com)
  • The increase of 2',5'-OAS activity and IFN titer in lymphocytes of irradiated animals may be regarded as a protective post radiation reaction of cells. (oalib.com)
  • The increased 2',5'-OAS activity and IFN titer in lymphocytes after their pre-incubation with IFN inducers can be considered as intensification of cellular protective postradiation mechanisms. (oalib.com)
  • Lymphocytic 2',5'-oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and immunoglobulin M and immunoglobulin G antibodies after primary and secondary immunization with yellow fever vaccine. (asm.org)
  • In the primary and secondary (memory) immune responses, 2',5'AS activity is expressed in the T-lymphocyte fraction prior to the appearance of antibodies directed against the virus and may serve as an early and sensitive marker of an ongoing virus infection which is otherwise difficult to detect. (asm.org)
  • If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. (bioxys.com)
  • Membrane-bound GP has been proposed to mediate cytotoxicity in endothelial cells ( 4 ), whereas soluble GP has been proposed to inhibit early neutrophil activation ( 5 ). (pnas.org)
  • 2',5'-0ligo(A): A Mediator of Viral RNA Cleavage in Interferon-Treated Cells? (elsevier.com)
  • The progression towards type 2 diabetes depends on the allostatic response of pancreatic beta cells to synthesise and secrete enough insulin to compensate for insulin resistance. (biomedcentral.com)
  • Tumors are further hypothesized to be sustained by the subpopulation of cancer stem cells [ 5 ], which should be specifically targeted for long-term successful therapeutic intervention. (biomedcentral.com)
  • By day 4 after the first immunization, the earliest and highest 2',5'AS activity was present in the T-lymphocyte fraction. (asm.org)
  • After the second immunization, performed 2 years +/- 2 months later, the only significant increase in 2',5'AS activity was observed in the T-lymphocyte fraction. (asm.org)
  • The 2',5'-OAS activity exceeded a control at all applied doses, the maximum level in splenocytes corresponded to 0.25 Gy dose whereas the highest values in thymocytes were revealed at 0.5-1.0 Gy doses. (oalib.com)
  • Enzymatic activity of 2'-5'-oligoadenylate synthetase is impaired by specific mutations that affect oligomerization of the protein. (semanticscholar.org)
  • These results indicate that tetramerization of the protein is required for the enzymatic activity of the small 2-5(A) synthetases. (semanticscholar.org)
  • Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. (abcam.com)
  • For example, the herpes simplex virus (HSV-1) protein ICP34.5 counteracts the PKR-mediated phosphorylation of translation initiation factor eIF-2α, preventing the establishment of an IFN-induced block in protein synthesis ( 11 ). (pnas.org)
  • Prognostic impact of Bcl-2 depends on tumor histology and expression of MALAT-1 lncRNA in non-small-cell lung cancer. (semanticscholar.org)
  • Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. (uniprot.org)
  • Mutations in human leucine-rich repeat kinase 2 (Lrrk2), a protein of yet unknown function, are linked to Parkinson's disease caused by degeneration of midbrain dopaminergic neurons. (mcponline.org)
  • The pathologic features and the immune responses characteristic of fatal and nonfatal human Ebola virus infections have begun to be characterized ( 3 - 5 ). (pnas.org)