2',5'-Oligoadenylate Synthetase: An enzyme that catalyzes the conversion of ATP into a series of (2'-5') linked oligoadenylates and pyrophosphate in the presence of double-stranded RNA. These oligonucleotides activate an endoribonuclease (RNase L) which cleaves single-stranded RNA. Interferons can act as inducers of these reactions. EC 2.7.7.-.Oligoribonucleotides: A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Interferons: Proteins secreted by vertebrate cells in response to a wide variety of inducers. They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions.Porifera: The phylum of sponges which are sessile, suspension-feeding, multicellular animals that utilize flagellated cells called choanocytes to circulate water. Most are hermaphroditic. They are probably an early evolutionary side branch that gave rise to no other group of animals. Except for about 150 freshwater species, sponges are marine animals. They are a source of ALKALOIDS; STEROLS; and other complex molecules useful in medicine and biological research.Adenine NucleotidesEndoribonucleases: A family of enzymes that catalyze the endonucleolytic cleavage of RNA. It includes EC 3.1.26.-, EC 3.1.27.-, EC 3.1.30.-, and EC 3.1.31.-.RNA, Double-Stranded: RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.Enzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.Interferon-alpha: One of the type I interferons produced by peripheral blood leukocytes or lymphoblastoid cells. In addition to antiviral activity, it activates NATURAL KILLER CELLS and B-LYMPHOCYTES, and down-regulates VASCULAR ENDOTHELIAL GROWTH FACTOR expression through PI-3 KINASE and MAPK KINASES signaling pathways.Interferon Type I: Interferon secreted by leukocytes, fibroblasts, or lymphoblasts in response to viruses or interferon inducers other than mitogens, antigens, or allo-antigens. They include alpha- and beta-interferons (INTERFERON-ALPHA and INTERFERON-BETA).eIF-2 Kinase: A dsRNA-activated cAMP-independent protein serine/threonine kinase that is induced by interferon. In the presence of dsRNA and ATP, the kinase autophosphorylates on several serine and threonine residues. The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis.Interferon-beta: One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Antiviral Agents: Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cell Line: Established cell cultures that have the potential to propagate indefinitely.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Glutamate-Ammonia Ligase: An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.Amino Acyl-tRNA Synthetases: A subclass of enzymes that aminoacylate AMINO ACID-SPECIFIC TRANSFER RNA with their corresponding AMINO ACIDS.Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Kinetics: The rate dynamics in chemical or physical systems.Poly I-C: Interferon inducer consisting of a synthetic, mismatched double-stranded RNA. The polymer is made of one strand each of polyinosinic acid and polycytidylic acid.Peptide Synthases: Ligases that catalyze the joining of adjacent AMINO ACIDS by the formation of carbon-nitrogen bonds between their carboxylic acid groups and amine groups.Coenzyme A Ligases: Enzymes that catalyze the formation of acyl-CoA derivatives. EC 6.2.1.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Immunity, Innate: The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.West Nile virus: A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE). It can infect birds and mammals. In humans, it is seen most frequently in Africa, Asia, and Europe presenting as a silent infection or undifferentiated fever (WEST NILE FEVER). The virus appeared in North America for the first time in 1999. It is transmitted mainly by CULEX spp mosquitoes which feed primarily on birds, but it can also be carried by the Asian Tiger mosquito, AEDES albopictus, which feeds mainly on mammals.Codon, Terminator: Any codon that signals the termination of genetic translation (TRANSLATION, GENETIC). PEPTIDE TERMINATION FACTORS bind to the stop codon and trigger the hydrolysis of the aminoacyl bond connecting the completed polypeptide to the tRNA. Terminator codons do not specify amino acids.West Nile Fever: A mosquito-borne viral illness caused by the WEST NILE VIRUS, a FLAVIVIRUS and endemic to regions of Africa, Asia, and Europe. Common clinical features include HEADACHE; FEVER; maculopapular rash; gastrointestinal symptoms; and lymphadenopathy. MENINGITIS; ENCEPHALITIS; and MYELITIS may also occur. The disease may occasionally be fatal or leave survivors with residual neurologic deficits. (From Joynt, Clinical Neurology, 1996, Ch26, p13; Lancet 1998 Sep 5;352(9130):767-71)Nod2 Signaling Adaptor Protein: A NOD signaling adaptor protein that contains two C-terminal leucine-rich domains which recognize bacterial PEPTIDOGLYCAN. It signals via an N-terminal capase recruitment domain that interacts with other CARD SIGNALING ADAPTOR PROTEINS such as RIP SERINE-THEONINE KINASES. The protein plays a role in the host defense response by signaling the activation of CASPASES and the MAP KINASE SIGNALING SYSTEM. Mutations of the gene encoding the nucleotide oligomerization domain 2 protein have been associated with increased susceptibility to CROHN DISEASE.ArchivesAcetylmuramyl-Alanyl-Isoglutamine: Peptidoglycan immunoadjuvant originally isolated from bacterial cell wall fragments; also acts as pyrogen and may cause arthritis; stimulates both humoral and cellular immunity.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Proline: A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.BooksMolecular Weight: The sum of the weight of all the atoms in a molecule.Heparin, Low-Molecular-Weight: Heparin fractions with a molecular weight usually between 4000 and 6000 kD. These low-molecular-weight fractions are effective antithrombotic agents. Their administration reduces the risk of hemorrhage, they have a longer half-life, and their platelet interactions are reduced in comparison to unfractionated heparin. They also provide an effective prophylaxis against postoperative major pulmonary embolism.Allied Health Occupations: Occupations of medical personnel who are not physicians, and are qualified by special training and, frequently, by licensure to work in supporting roles in the health care field. These occupations include, but are not limited to, medical technology, physical therapy, physician assistant, etc.UracilRNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Library Collection Development: Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Sleep: A readily reversible suspension of sensorimotor interaction with the environment, usually associated with recumbency and immobility.Sleep, REM: A stage of sleep characterized by rapid movements of the eye and low voltage fast pattern EEG. It is usually associated with dreaming.Amyotrophic Lateral Sclerosis: A degenerative disorder affecting upper MOTOR NEURONS in the brain and lower motor neurons in the brain stem and SPINAL CORD. Disease onset is usually after the age of 50 and the process is usually fatal within 3 to 6 years. Clinical manifestations include progressive weakness, atrophy, FASCICULATION, hyperreflexia, DYSARTHRIA, dysphagia, and eventual paralysis of respiratory function. Pathologic features include the replacement of motor neurons with fibrous ASTROCYTES and atrophy of anterior SPINAL NERVE ROOTS and corticospinal tracts. (From Adams et al., Principles of Neurology, 6th ed, pp1089-94)Sleep Disorders: Conditions characterized by disturbances of usual sleep patterns or behaviors. Sleep disorders may be divided into three major categories: DYSSOMNIAS (i.e. disorders characterized by insomnia or hypersomnia), PARASOMNIAS (abnormal sleep behaviors), and sleep disorders secondary to medical or psychiatric disorders. (From Thorpy, Sleep Disorders Medicine, 1994, p187)Diaphragm: The musculofibrous partition that separates the THORACIC CAVITY from the ABDOMINAL CAVITY. Contraction of the diaphragm increases the volume of the thoracic cavity aiding INHALATION.Neuromuscular Diseases: A general term encompassing lower MOTOR NEURON DISEASE; PERIPHERAL NERVOUS SYSTEM DISEASES; and certain MUSCULAR DISEASES. Manifestations include MUSCLE WEAKNESS; FASCICULATION; muscle ATROPHY; SPASM; MYOKYMIA; MUSCLE HYPERTONIA, myalgias, and MUSCLE HYPOTONIA.Wakefulness: A state in which there is an enhanced potential for sensitivity and an efficient responsiveness to external stimuli.
... oligoadenylate synthetase-like 2 is a protein that in the house mouse is encoded by the Oasl2 gene. The gene is also known as ... oligoadenylate synthetase with a ubiquitin-like extension, is induced during maturation of murine dendritic cells". Journal of ... oligoadenylate synthetase-like 2". Retrieved 2011-12-11. "Salmonella infection data for Oasl2". Wellcome Trust Sanger Institute ... oligoadenylate synthetase gene family". Molecular Biology and Evolution. 17 (5): 738-750. doi:10.1093/oxfordjournals.molbev. ...
... oligoadenylate synthetase-like protein is an enzyme that in humans is encoded by the OASL gene. 2'-5'-oligoadenylate synthase ... oligoadenylate synthetase-like". Mackay V, Linn S (1976). "Selective inhibition of the dnase activity of the recBC enzyme by ... oligoadenylate synthetase-like gene (OASL) encoding the interferon-induced 56-kDa protein maps to chromosome 12q24.2 in the ... oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1". Eur. J. ...
... oligoadenylate synthetase 3 is an enzyme that in humans is encoded by the OAS3 gene. This gene encodes an enzyme included in ... oligoadenylate synthetase". Genomics. 70 (2): 232-40. doi:10.1006/geno.2000.6382. PMID 11112351. Ito M, Shichijo S, Tsuda N, et ... oligoadenylate synthetase locus is composed of three distinct genes clustered on chromosome 12q24.2 encoding the 100-, 69-, and ... oligoadenylate synthetase catalyzing preferentially the synthesis of dimeric pppA2'p5'A molecules is composed of three ...
... oligoadenylate synthetase 1 is an enzyme that in humans is encoded by the OAS1 gene. This gene encodes a member of the 2-5A ... oligoadenylate synthetase 1, 40/46kDa". Justesen J, Hartmann R, Kjeldgaard NO (2000). "Gene structure and function of the 2'-5 ... oligoadenylate synthetase". J. Biochem. 99 (5): 1455-64. PMID 3754863. Merlin G, Chebath J, Benech P, et al. (1983). "Molecular ... oligoadenylate synthetase is impaired by specific mutations that affect oligomerization of the protein". J. Biol. Chem. 272 (52 ...
... oligoadenylate synthetase 2 is an enzyme that in humans is encoded by the OAS2 gene. This gene encodes a member of the 2-5A ... oligoadenylate synthetase 2, 69/71kDa". Justesen J, Hartmann R, Kjeldgaard NO (2000). "Gene structure and function of the 2'-5 ... oligoadenylate synthetase locus is composed of three distinct genes clustered on chromosome 12q24.2 encoding the 100-, 69-, and ... oligoadenylate synthetase family". Cell. Mol. Life Sci. 57 (11): 1593-1612. doi:10.1007/PL00000644. PMID 11092454. Marié I, ...
Oligoadenylate Synthetase (OAS). The transcribed RNA is then spliced and modified in the nucleus before reaching the cytoplasm ... oligoadenylate synthetase-dependent ribonuclease - is an interferon (IFN)-induced ribonuclease which, upon activation, destroys ... linked oligoadenylates (2-5A), which are 5' end phosphorylated. 2-5 A molecules then bind to RNase L, promoting its activation ... oligoadenylate depends on the post-transcriptional and post-translational modifications of OAS. OAS is only activated under a ...
... oligoadenylate synthetase". Eur. J. Biochem. 257 (2): 319-30. doi:10.1046/j.1432-1327.1998.2570319.x. PMID 9826176. Guo J, Sen ... 267 (2): 209-19. doi:10.1006/viro.1999.0135. PMID 10662616. Guo J, Hui DJ, Merrick WC, Sen GC (2001). "A new pathway of ... 13 (2): 458-60. doi:10.1016/0888-7543(92)90272-T. PMID 1377167. Wathelet MG, Clauss IM, Content J, Huez GA (Jun 1988). "The IFI ... 169 (2): 313-21. doi:10.1111/j.1432-1033.1987.tb13614.x. PMID 3121313. Wathelet MG, Szpirer J, Nols CB, et al. (1988). "Cloning ...
... oligoadenylate synthetase family, which degrades viral RNA. Some viruses evade this by producing molecules which interfere with ... 5 (6): 1003-1011. doi:10.1016/S1097-2765(00)80265-8. PMID 10911994. Rojo, E.; et al. (2004). "VPEgamma exhibits a caspase-like ... 5 (4): 331-42. doi:10.1038/nri1594. PMID 15803152. Janeway, C.A.; Travers, P.; Walport, M.; Shlomchik, M.J. (2005). ... 117 (2): 289-96. doi:10.1172/JCI30555. PMC 1783813 . PMID 17273548. Tracey, KJ (2009). "Reflex control of immunity". Nature ...
... oligoadenylate synthetase as well as ISGF3G (Interferon Stimulated Gene Factor 3). IL-28A and IL-28B belong to the type III ... 17 (2): 103-6. PMID 15171810. Sheppard P, Kindsvogel W, Xu W, Henderson K, Schlutsmeyer S, Whitmore TE, Kuestner R, Garrigues U ...
... oligoadenylate synthetase MeSH D08.811.913.696.445.650 --- polynucleotide adenylyltransferase MeSH D08.811.913.696.445.692 --- ... amino acyl-trna synthetases MeSH D08.811.464.263.200.050 --- alanine-tRNA ligase MeSH D08.811.464.263.200.100 --- arginine-tRNA ... atp synthetase complexes MeSH D08.811.913.696.650.150.500 --- proton-translocating atpases MeSH D08.811.913.696.650.150.500.249 ... fatty acid synthetase complex MeSH D08.811.600.391 --- glycine decarboxylase complex MeSH D08.811.600.391.100 --- ...
... oligoadenylate synthetase is an enzyme. It is an antiviral enzyme that counteracts viral attack by degrading viral and host RNA ... oligoadenylate synthetase, also referred to as domain 2 of the enzyme, is largely alpha-helical and homologous to a tandem ... oligoadenylate synthetase". Mol. Cell. 12 (5): 1173-85. doi:10.1016/S1097-2765(03)00433-7. PMID 14636576. This article ... oligoadenylate synthetases. Structure-function relationships". J. Biol. Chem. 266 (23): 15293-9. PMID 1651324. Hartmann R, ...
... members of the oligoadenylate synthetase family of proteins (OAS1, OAS2, OAS3), and others. All of these proteins can ... 23 (2): 165-175. doi:10.1016/j.immuni.2005.06.008. PMID 16111635. Diebold SS, Kaisho T, Hemmi H, Akira S, Reis e Sousa C (2004 ... 29 (2): 178-181. doi:10.1016/j.immuni.2008.07.009. PMID 18701081. Schmidt A, Schwerd T, Hamm W, Hellmuth JC, Cui S, et al. ( ... 5 (7): 730-737. doi:10.1038/ni1087. PMID 15208624. Das HK, Das A, Ghosh-Dastidar P, Ralston RO, Yaghmai B, et al. (1981). " ...
... synthetase Polyadenylate nucleotidyltransferase Polyadenylate polymerase Polyadenylate synthetase Polyadenylic acid polymerase ... Mans RJ, Walter TJ (1971). "Transfer RNA-primed oligoadenylate synthesis in maize seedlings. II Primer, substrate and metal ... U.S.A. 69 (2): 417-21. doi:10.1073/pnas.69.2.417. PMC 426470 . PMID 4501121. Colgan DF, Murthy KG, Prives C, Manley JL ( ... 384 (6606): 282-5. doi:10.1038/384282a0. PMID 8918882. Molecular and Cellular Biology portal. ...
Aminoacyl tRNA synthetases, class I. *Aminoacyl tRNA synthetases, class II. *Aminotransferase, class V ... oligoadenylate synthase. *3-dehydroquinate dehydratase. *3-dehydroquinate synthase. *DAHP synthase. *3-hydroxy-3-methylglutaryl ...
... oligoadenylate synthetase (2-5 AS) and to measure the 2-5AS in peripheral blood mononuclear cell (PBMC) extracts of patients ... oligoadenylate synthetase (2-5 AS) and to measure the 2-5AS in peripheral blood mononuclear cell (PBMC) extracts of patients ... oligoadenylate synthetase and its application in chronic hepatitis C patients receiving interferon-alpha. ... The 2-5AS activity of PBMC in normal persons was in a quite low level (2.0%), and it was increased about ten-folds after ...
... oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis. Kirje Kelve, M.; Truve, E.; Aaspõllu ... oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis. Müller, W. E. G.. Use of Aquatic ... oligoadenylate synthetase mRNA level in rat pheochromocytoma PC12 cells during apoptosis ... A.; Kuusksalu, A.; Dapper, J.; Perovic, S.; Müller, W. E. G. (1994). Reduction of 2-5- ...
... oligoadenylate synthetase 1 (OAS1) and ribonuclease L (RNASEL) innate immunity genes. *Jonathan J Rios1. , ... oligoadenylate synthetase/L1 isoform is associated with West Nile virus susceptibility in laboratory mice. Proc Natl Acad Sci ... oligoadenylate synthetase (25AS) enzyme activity is controlled by a single-nucleotide polymorphism at a splice-acceptor site ... The small synthetases are transcribed from the OAS1 gene while the medium and large synthetases are encoded by the OAS2 and ...
... oligoadenylate synthetase (2,5AS) activity, determined in homogenates of peripheral B and T lymphocytes. From cellular ATP, ... oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and immunoglobulin M and ... oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and immunoglobulin M and ... oligoadenylate synthetase activity increases prior to the appearance of neutralizing antibodies and immunoglobulin M and ...
oligoadenylate synthetases. OAS2. 2-5- oligoadenylate synthetase type 2. poly(I:C). polyinosinic acid : polycytidylic acid. ... Oligoadenylate Synthetase Type 2 and Enhances RNase-L Function in THP-1 Cells. Jae W. Dugan,1,2,# Amador Albor,1,2,# Larry ... oligoadenylate synthetase by the herpes simplex virus type 1 Us11 protein. J Virol. 2007;81:3455-3464. [PMC free article] [ ... oligoadenylate synthetase. J Biol Chem. 1999;274:1848-1855. [PubMed]. *Silverman RH, Skehel JJ, James TC, Wreschner DH, Kerr IM ...
Oligoadenylate Synthetase 1, 40/46kDa, His-tagged. Download Datasheet See All OAS1 Products. Bring this labeled protein ... oligoadenylate synthetase 1; (2-5")oligo(A) synthetase 1; 2-5A synthetase 1; p46/p42 OAS; E18/E16; OAS1; OIAS; IFI-4; OIASI; 2- ... oligoadenylate synthetase 1, 40/46kDa; 2"-5" oligoadenylate synthetase 1 p48 isoform; 2"-5" oligoadenylate synthetase 1 p52 ... OAS1 enzyme is a member of the 2μ,5μ-oligoadenylate synthetase family. OAS1 is induced by interferons and uses adenosine ...
... oligoadenylate synthetase (2,5-OAS) activity and interferon (IFN) level in lymphocytes of rats irradiated with 0.25, 0.5, ... Murine Pancreatic Beta TC3 Cells Show Greater -Oligoadenylate Synthetase ( AS) Antiviral Enzyme Activity and Apoptosis ... oligoadenylate synthetase (2,5-OAS) activity and interferon (IFN) level in lymphocytes of rats irradiated with 0.25, 0.5, ... Oligoadenylate Synthetase by Stem Loops at the 5′-End of the West Nile Virus Genome. ...
... oligoadenylate synthetases (OAS) are antiviral proteins and several isoforms have been identified as flavivirus-resistance ... Porcine 2′, 5′-Oligoadenylate Synthetases Inhibit Japanese Encephalitis Virus Replication In Vitro. ...
... oligoadenylate synthetase by stem loops at the 5′-end of the West Nile virus genome. PLoS ONE, 9 (3). e92545/1-e92545/11. ISSN ... oligoadenylate synthetase by stem loops at the 5′-end of the West Nile virus genome. PLoS ONE 9(3): e92545. doi:10.1371/journal ... oligoadenylate synthetases (OAS) are key ISGs that help to amplify the innate immune response. Upon interaction with viral ... oligoadenylate synthetase by stem loops at the 5′-end of the West Nile virus genome ...
... linked oligoadenylates that activate RNase L and cause mRNA degradation. Because they all can synthesize 2-5(A), the reason for ... synthetase has an additional activity: it promotes apoptosis in mammalian cells. The proapoptotic activity of 9-2 was isozyme- ... synthetases are a family of interferon-induced enzymes that polymerize ATP into 2-5 ... The 9-2-expressing cells exhibited many properties of cells undergoing apoptosis, such as DNA fragmentation, caspase activation ...
Oligoadenylate Synthetase 2 (OAS2) ELISA kit, Cat#EKU02016. Write a Review Write a Review. × ... Oligoadenylate Synthetase 2 (OAS2) ELISA kit, Cat#EKU02016. Rating Required Select Rating. 1 star (worst). 2 stars. 3 stars ( ... Oligoadenylate Synthetase 2 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV ... Oligoadenylate Synthetase 2 and analogues was observed.. Assay Procedure. 1.Prepare all reagents, samples and standards; 2.Add ...
Oligoadenylate Synthetase 2 OAS2 Polyclonal Antibody 01012487543 at Gentaur 2,5 Oligoadenylate Synthetase 2 (OAS2) ... Oligoadenylate Synthetase 2 (OAS2) Polyclonal Antibody. View details of this awesome product ... If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5° ... 2,3-Cyclic Nucleotide 3-Phosphodiesterase (CNP) Polyclonal Antibody. See more details ...
Oligoadenylate synthetase 1 polymorphisms are associated with tuberculosis: a case-control study.. Wu S1, Wang Y1, Chen G1,2, ... Oligoadenylate synthetase 1 (OAS1) plays an important role in inflammatory immune reactions. OAS1 polymorphisms have been ... Oligoadenylate synthetase 1 polymorphisms are associated with tuberculosis: a case-control study ... Oligoadenylate synthetase 1 polymorphisms are associated with tuberculosis: a case-control study ...
... oligoadenylate synthetase;. ISRE,. IFN-stimulated response element;. GP,. glycoprotein;. pfu,. plaque-forming units;. moi,. ... oligoadenylate synthetase (OAS), and the Mx proteins (7-10).. Many viruses have evolved mechanisms to subvert the host IFN ... 1994) in Encyclopedia of Virology, eds Webster R G, Granoff A (Academic, New York), 2, pp 827-831. ... 5). Further, coexpression of VP35 and NP was able to block ISRE activation to the same extent as expression of VP35 alone (Fig ...
... synthetase causes a loss of its enzyme activity (Ghosh, S. K., Kusari, J., Bandyopadhyay, S. K., Samanta, H., Kumar, R., and ... synthetases revealed that the residues at positions 330 to 333 are highly conserved. Alanine-scanning mutagenesis of these ... Previous studies from our laboratory have shown that deletion of residues 321 to 344 of the 9-2 isozyme of 2-5-oligoadenylate ... These results indicate that tetramerization of the protein is required for the enzymatic activity of the small 2-5(A) ...
2014 Sep-Oct;18(5):548-50. doi: 10.1016/j.bjid.2014.03.004. Epub 2014 May 9. Research Support, Non-U.S. Govt ... Single nucleotide polymorphisms in the oligoadenylate synthetases genes are known to affect oligoadenylate synthetases activity ... Oligoadenylate synthetases play an important role in the immune response against dengue virus. ... oligoadenylate synthetase genes: a case-control study.. Thamizhmani R1, Vijayachari P2. ...
... oligoadenylate synthetase were higher in cell samples treated with IFN-alpha5 than with IFN-alpha2. These findings suggest that ... oligoadenylate synthetase than IFN-alpha2 in liver cells. J Interferon Cytokine Res 2004 Aug;24(8):497-503. ... Oligoadenylate synthetase/metabolism. DNA-Binding proteins/metabolism. Hepatocytes/drug effects. Interferon-alpha/pharmacology ... IFN-alpha5 mediates stronger Tyk2-stat-dependent activation and higher expression of 2,5-oligoadenylate synthetase than IFN- ...
... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor. Molecular and Cellular Biology, 9( ... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor. Mariano Garcia-Blanco, P. Lengyel ... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor, Molecular and Cellular Biology, ... oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor. In: Molecular and Cellular Biology ...
... oligoadenylate synthetase. CHB. chronic hepatitis B. HCC. hepatocellular carcinoma. 2-DE. two-dimensional gel electrophoresis. ... oligoadenylate synthetase and RNase L signaling pathway was noted to be activated in GRP78-overexpressing HepG2 cells. Moreover ... oligoadenylate synthetase-RNase L pathway in hepatocytes. Induction of hepatic GRP78 may provide a novel therapeutic approach ... 2. Identification of GRP78 (spot 26) by MALDI-TOF MS and MS/MS analysis. A, MALDI-TOF MS spectrum of GRP78 labeled with masses ...
... oligoadenylate synthetase. P:A ratio. perimeter to area ratio. PIE. physical interaction enrichment. PPI. protein-protein ... oligoadenylate synthetase) mRNA expression (24). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)-forward (5′- ... 5C). However, the distribution of the P:A-ratio revealed a shift toward higher values in the Lrrk2 knockdown cultures (Fig. 5C ... 5A). As a quantitative measure of cell shape, we determined the ratio of perimeter to area (P:A-ratio) (38) and calculated both ...
... oligoadenylate synthetase (2, 5-OAS) in ACHN renal cell carcinoma cells. In terms of all three activities, the nine subtypes ... Their relative effects on cell growth were similar in two other human cell lines, SK-LU-1 lung cancer cells and KU-2 renal cell ...
Oligoadenylate Synthetase. Mechanism of Action of 2-5A in Intact Cells. 2,5-0ligo(A): A Mediator of Viral RNA Cleavage in ... 2. Effect of ppGpp on RNA Polymerase and Transcription in E. Coli. Molecular Interactions in the Initiation of rRNA Synthesis ... Guanosine 3,5-Bis (Diphosphate) Search in Eukaryotes. II. Purine Nucleotides and Sporulation. Initiation of Bacterial and ... Guanosine 3,5-Bispyrophosphate is a Dispensable Metabolite. Studies on the Coordination of tRNA-Charging and Polypeptide ...
... oligoadenylate synthetase 1H MGI:2180853 .yui-skin-sam .yui-dt th{ background:url(http://www.informatics.jax.org/webshare/ ...
... oligoadenylate synthetase 1H isoform 1 [Mus musculus]. Provider RefSeq Sequence Polypeptide 369 aa ...
... oligoadenylate synthetase 1; an interferon-induced antiviral protein) have also been associated with SARS susceptibility and ... 2. ,. 3 and Yoav Keynan. 1. ,. 2. ,. 3. ,. 4. 1Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada ... 5, pp. 672-678, 2010. View at: Publisher Site , Google Scholar*J. F.-W. Chan, K. K.-W. To, H. Tse et al., "The lower serum ... 2, pp. 582-590, 2002. View at: Publisher Site , Google Scholar*W. K. E. Ip, H. C. Kwok, H. K. W. Law et al., "Mannose-binding ...
  • Here we report that the 9-2 isozyme of 2-5(A) synthetase has an additional activity: it promotes apoptosis in mammalian cells. (semanticscholar.org)
  • Fukamachi Y, Karasaki Y, Sugiura T, Itoh H, Abe T, Yamamura K, Higashi K (1998) Zinc suppresses apoptosis of U937 cells induced by hydrogen peroxide through an increase of the Bcl-2/Bax ratio. (springer.com)
  • Kim NS, Lee GM (2000) Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production. (springer.com)
  • OAS3 is an interferon inducible protein that belongs to the 2-5A synthetase family, may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. (acris-antibodies.com)
  • From cellular ATP, this enzyme generates 2',5'-oligoadenylates which mediate degradation of viral mRNA by stimulation of a latent RNase. (asm.org)
  • 2',5'-0ligo(A): A Mediator of Viral RNA Cleavage in Interferon-Treated Cells? (elsevier.com)
  • Many hosts also withstand comparable level of viral insult with little or no overt symptoms, exhibiting a higher degree of tolerance [2] , . (plos.org)
  • Viral myocarditis has occurred in an estimated 5 to 20% of the human population. (asm.org)
  • Despite the relevance of viral infection for human health, only few genome-wide association studies (GWAS) have been performed in the attempt to identify variants associated with increased susceptibility to infection or faster disease progression - [5] . (prolekare.cz)
  • However, in 2-3% of young children it leads to severe bronchiolitis and viral pneumonia, and it remains the major cause of infant hospitalization in the developed world. (rupress.org)
  • For example, the expression of interferons (IFNs), proinflammatory cytokines, and chemokines at the mucosal challenge site can be positively associated with viral replication ( 4 , 5 ). (pubmedcentralcanada.ca)
  • 1. Fankhauser, H., Schiff, J.A. and Garber, L.J. Purification and properties of adenylyl sulphate:ammonia adenylyltransferase from Chlorella catalysing the formation of adenosine 5′ -phosphoramidate from adenosine 5′ -phosphosulphate and ammonia. (qmul.ac.uk)
  • We tested their inhibitory effects on cell growth and replication of Semliki Forest virus (SFV) and vesicular stomatitis virus (VSV) and their induction of 2',5'-oligoadenylate synthetase (2', 5'-OAS) in ACHN renal cell carcinoma cells. (nih.gov)
  • The binding of recombinant leukocyte clone A IFN and the induction of 2′,5′-oligoadenylate synthetase (2,5A) in peripheral blood cells were studied to determine whether they correlate with clinical response to IFN therapy. (aacrjournals.org)
  • In a recent study, using an in vivo mouse model for genital herpes, we demonstrated an essential role of DCs in Th1 induction following HSV-2 infection in the vaginal mucosa ( 2 ). (rupress.org)
  • Burysek L, Pitha PM (2001) Latently expressed human herpesvirus 8-encoded interferon regulatory factor 2 inhibits double-stranded RNA-activated protein kinase. (springer.com)
  • 2000 Feb;107(2):147-52, 155, 158-9. (uwgi.org)
  • Prognostic impact of Bcl-2 depends on tumor histology and expression of MALAT-1 lncRNA in non-small-cell lung cancer. (semanticscholar.org)
  • Next, we identified that IFN-α secretion by pDCs required the expression of the adaptor molecule MyD88, suggesting the involvement of a Toll-like receptor (TLR) in HSV-2 recognition. (rupress.org)
  • 6. A DNA molecule according to claim 2 which is comprised in an expression vector. (google.com)
  • The RNase L protein consists of three domains: 1) an N-terminal domain of ankyrin repeats with P-loop motifs between the seventh and eighth repeat, 2) a serine/threonine protein kinase domain, and 3) a C-terminal ribonuclease domain [ 27 ]. (biomedcentral.com)
  • Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. (uniprot.org)
  • Primary and secondary immunizations with live, attenuated yellow fever virus vaccine (17D strain) were performed in order to study the course of appearance of virus-neutralizing antibodies and immunoglobulin M (IgM) and IgG antibodies directed against the virus and the interferon-dependent enzyme 2',5'-oligoadenylate synthetase (2',5'AS) activity, determined in homogenates of peripheral B and T lymphocytes. (asm.org)
  • PKR interferes with virus propagation by phosphorylating the α subunit of translation initiation factor 2 (eIF2α), converting eIF2 from a substrate to an inhibitor of its guanine nucleotide exchange factor, eIF2B. (asm.org)
  • In the primary and secondary (memory) immune responses, 2',5'AS activity is expressed in the T-lymphocyte fraction prior to the appearance of antibodies directed against the virus and may serve as an early and sensitive marker of an ongoing virus infection which is otherwise difficult to detect. (asm.org)
  • Consistent with the ability of these cells to induce T cell activation, the DCs in the draining lymph nodes were found to increase their levels of costimulatory molecules as early as 24 h after intravaginal infection with HSV-2 ( 2 ). (rupress.org)
  • Viraemia during acute RV infection is more commonly detected in children with acute asthma exacerbations than in non-asthmatic children with colds 5 , suggesting that asthma is associated with a reduced ability to limit systemic spread of virus. (ersjournals.com)
  • The simian immunodeficiency virus (SIV) infection of rhesus macaques is an excellent model to assess these different tissue sites permitting both virologic and immunologic assessment of critical tissue compartments ( 1 , 5 , 21 , 40 , 66 ). (pubmedcentralcanada.ca)
  • This model has been particularly useful in assessing the role of cytokine and chemokine responses in different tissue sites at early times after SIV infection ( 4 , 5 , 40 ). (pubmedcentralcanada.ca)
  • LN and lungs) has been observed during acute and chronic SIV infection ( 1 , 5 , 55 ). (pubmedcentralcanada.ca)
  • Inhaled IFNs have proven to be well tolerated and beneficial for rhinovirus infection ( 4 ) and pulmonary tuberculosis ( 5 ). (cdc.gov)
  • Toward developing vaccines to prevent HSV-2 transmission, a clear understanding of the mechanism of how immunity is generated within the relevant mucosal sites is necessary. (rupress.org)
  • RAF-1 is capable of activating the NF-kB transcription factor through an unknown mechanism that does not seem to involve direct phosphorylation of NF-kB and is independent of MEK1/2-ERK1/2 signaling. (atlasgeneticsoncology.org)
  • Nucleotide-binding and oligomerization domain 2 (NOD2), an NLR family member, is an intracellular protein that is activated by muramyl dipeptide (MDP), a breakdown product of bacterial peptidoglycan. (pubmedcentralcanada.ca)
  • Membrane-bound GP has been proposed to mediate cytotoxicity in endothelial cells ( 4 ), whereas soluble GP has been proposed to inhibit early neutrophil activation ( 5 ). (pnas.org)
  • The downstream effects of MEK1/2-ERK1/2 activation are varied, complex, and depend on the cellular context. (atlasgeneticsoncology.org)
  • The pathologic features and the immune responses characteristic of fatal and nonfatal human Ebola virus infections have begun to be characterized ( 3 - 5 ). (pnas.org)
  • 2',5'-Oligoadenylate and 2',5'-oligoadenylate phosphodiesterase in human plasma. (biomedsearch.com)
  • 2',5'-Oligoadenylate and 2',5'-oligoadenylate phosphodiesterase activity were detected in the human plasma and serum by sensitive radioimmuno assays. (biomedsearch.com)
  • Mutations in human leucine-rich repeat kinase 2 (Lrrk2), a protein of yet unknown function, are linked to Parkinson's disease caused by degeneration of midbrain dopaminergic neurons. (mcponline.org)
  • Their relative effects on cell growth were similar in two other human cell lines, SK-LU-1 lung cancer cells and KU-2 renal cell carcinoma cells, whereas cells of the Daudi Burkitt lymphoma line behaved quite differently, being highly sensitive to all the nine subtypes. (nih.gov)
  • In particular, viruses have affected humans before they emerged as a species, as testified by the fact that roughly 8% of the human genome is represented by recognizable endogenous retroviruses [2] which represent the fossil remnants of past infections. (prolekare.cz)
  • Following an incubation period of 2-21 days, human EBOV and MARV infections normally show an abrupt disease onset that is characterized by flu-like symptoms (fever, chills, malaise and myalgia). (biologists.org)
  • Burysek L, Yeow WS, Pitha PM (1999b) Unique properties of a second human herpesvirus 8-encoded interferon regulatory factor (vIRF-2). (springer.com)
  • 2. A DNA molecule encoding a human Tumor Necrosis Factor Stimulated Gene 6 (TSG-6), protein having the amino acid sequence SEO ID NO:2, substantially free of other human nucleotide sequences. (google.com)
  • The human double-stranded RNA (dsRNA)-dependent protein kinase PKR inhibits protein synthesis by phosphorylating translation initiation factor 2α (eIF2α). (asm.org)
  • Specifically, CD11b + DCs were found to present virus antigens to CD4 + T cells in the draining lymph nodes, and this interaction was crucial in the generation of the potent Th1 response ( 2 ) needed to provide protective immunity. (rupress.org)