15-Oxoprostaglandin 13-Reductase: (5Z)-(15S)-11 alpha-Hydroxy-9,15-dioxoprostanoate:NAD(P)+ delta(13)-oxidoreductase. An enzyme active in prostaglandin E and F catabolism. It catalyzes the reduction of the double bond at the 13-14 position of the 15-ketoprostaglandins and uses NADPH as cofactor. EC 1.3.1.48.Alcohol Dehydrogenase: A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.6-Ketoprostaglandin F1 alpha: The physiologically active and stable hydrolysis product of EPOPROSTENOL. Found in nearly all mammalian tissue.Prostaglandins: A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.Prostaglandins F: (9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.Thromboxane B2: A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).Dinoprost: A naturally occurring prostaglandin that has oxytocic, luteolytic, and abortifacient activities. Due to its vasocontractile properties, the compound has a variety of other biological actions.Prostaglandins E: (11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.Ginger: Deciduous plant rich in volatile oil (OILS, VOLATILE). It is used as a flavoring agent and has many other uses both internally and topically.Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Catechin: An antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms.Sulfotransferases: Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.Fatty Alcohols: Usually high-molecular-weight, straight-chain primary alcohols, but can also range from as few as 4 carbons, derived from natural fats and oils, including lauryl, stearyl, oleyl, and linoleyl alcohols. They are used in pharmaceuticals, cosmetics, detergents, plastics, and lube oils and in textile manufacture. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Glucuronosyltransferase: A family of enzymes accepting a wide range of substrates, including phenols, alcohols, amines, and fatty acids. They function as drug-metabolizing enzymes that catalyze the conjugation of UDPglucuronic acid to a variety of endogenous and exogenous compounds. EC 2.4.1.17.Alcohol Oxidoreductases: A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.District of Columbia: A federal area located between Maryland and Virginia on the Potomac river; it is coextensive with Washington, D.C., which is the capital of the United States.Databases, Factual: Extensive collections, reputedly complete, of facts and data garnered from material of a specialized subject area and made available for analysis and application. The collection can be automated by various contemporary methods for retrieval. The concept should be differentiated from DATABASES, BIBLIOGRAPHIC which is restricted to collections of bibliographic references.Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.SwitzerlandCD-ROM: An optical disk storage system for computers on which data can be read or from which data can be retrieved but not entered or modified. A CD-ROM unit is almost identical to the compact disk playback device for home use.Steroid Isomerases: Enzymes that catalyze the transposition of double bond(s) in a steroid molecule. EC 5.3.3.Testosterone: A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.Hydrogenase: An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.Cholesterol Oxidase: An enzyme that catalyzes the oxidation of cholesterol in the presence of molecular oxygen to 4-cholesten-3-one and hydrogen peroxide. The enzyme is not specific for cholesterol, but will also oxidize other 3-hydroxysteroids. EC 1.1.3.6.Brassinosteroids: Plant steroids ubiquitously distributed throughout the plant kingdom. They play essential roles in modulating growth and differentiation of cells at nanomolar to micromolar concentrations.Steroids: A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed)NADP: Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)Urinary Retention: Inability to empty the URINARY BLADDER with voiding (URINATION).Mars: The fourth planet in order from the sun. Its two natural satellites are Deimos and Phobos. It is one of the four inner or terrestrial planets of the solar system.Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Bacteriological Techniques: Techniques used in studying bacteria.Urinary Catheterization: Passage of a CATHETER into the URINARY BLADDER or kidney.Dementia, Vascular: An imprecise term referring to dementia associated with CEREBROVASCULAR DISORDERS, including CEREBRAL INFARCTION (single or multiple), and conditions associated with chronic BRAIN ISCHEMIA. Diffuse, cortical, and subcortical subtypes have been described. (From Gerontol Geriatr 1998 Feb;31(1):36-44)Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Northwest Territories: A federally administered division of Canada. Its capital is Yellowknife. The former northern and eastern-most parts of the Territory comprise the new territory of Nunavut, effective April 1, 1999.Jaundice, Chronic Idiopathic: A benign, autosomally recessive inherited hyperbilirubinemia characterized by the presence of a dark pigment in the centrilobular region of the liver cells. There is a functional defect in biliary excretion of bilirubin, cholephilic dyes, and porphyrins. Affected persons may be asymptomatic or have vague constitutional or gastrointestinal symptoms. The liver may be slightly enlarged, and oral and intravenous cholangiography fails to visualize the biliary tract.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Endocrinology: A subspecialty of internal medicine concerned with the metabolism, physiology, and disorders of the ENDOCRINE SYSTEM.Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.

Oxidoreductases in lipoxin A4 metabolic inactivation: a novel role for 15-onoprostaglandin 13-reductase/leukotriene B4 12-hydroxydehydrogenase in inflammation. (1/10)

The lipoxins (LX) are autacoids that act within a local inflammatory milieu to dampen neutrophil recruitment and promote resolution. 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) and 15-oxoprostaglandin 13-reductase, also termed leukotriene B(4) 12-hydroxydehydrogenase (PGR/LTB(4)DH), are two enzymatic activities appreciated for their roles in the metabolism of prostaglandins and LTB(4). Here, we determined whether these oxidoreductases also catalyze the conversion of lipoxin A(4) (LXA(4)) and assessed the activities of these LXA(4) metabolites. 15-Oxo-LXA(4) was generated by incubating LXA(4) with 15-PGDH and NAD(+) for studies of its further conversion. PGR/LTB(4)DH catalyzed the NADH-dependent reduction of 15-oxo-LXA(4) to yield 13,14-dihydro-15-oxo-LXA(4). With NADH as a cofactor, 15-PGDH acted as a 15-carbonyl reductase and catalyzed the conversion of 13,14-dihydro-15-oxo-LXA(4) to 13, 14-dihydro-LXA(4). Human polymorphonuclear leukocytes (PMN) exposed to native LXA(4), 15-oxo-LXA(4), or 13,14-dihydro-LXA(4) did not produce superoxide anions. At concentrations where LXA(4) and a metabolically stable LXA(4) analog potently inhibited leukotriene B(4)-induced superoxide anion generation, the further metabolites were devoid of activity. Neither 15-oxo-LXA(4) nor 13, 14-dihydro-LXA(4) effectively competed with (3)H-labeled LXA(4) for specific binding to recombinant LXA(4) receptor (ALXR). In addition, introducing recombinant PGR/LTB(4)DH into a murine exudative model of inflammation increased PMN number by approximately 2-fold, suggesting that this enzyme participates in the regulation of PMN trafficking. These results establish the structures of LXA(4) further metabolites and indicate that conversion of LXA(4) to oxo- and dihydro- products represents a mode of LXA(4) inactivation in inflammation. Moreover, they suggest that these eicosanoid oxidoreductases have multifaceted roles controlling the levels of specific eicosanoids involved in the regulation of inflammation.  (+info)

Antioxidative function and substrate specificity of NAD(P)H-dependent alkenal/one oxidoreductase. A new role for leukotriene B4 12-hydroxydehydrogenase/15-oxoprostaglandin 13-reductase. (2/10)

There are several known routes for the metabolic detoxication of alpha,beta-unsaturated aldehydes and ketones, including conjugation to glutathione and reduction and oxidation of the aldehyde to an alcohol and a carboxylic acid, respectively. In this study, we describe a fourth class of detoxication that involves the reduction of the alpha,beta-carbon=carbon double bond to a single bond. This reaction is catalyzed by NAD(P)H-dependent alkenal/one oxidoreductase (AO), an enzyme heretofore known as leukotriene B4 12-hydroxydehydrogenase, 15-oxoprostaglandin 13-reductase, and dithiolethione-inducible gene-1. AO is shown to effectively reduce cytotoxic lipid peroxidation products such as 4-hydroxy-2-nonenal (HNE) (k(cat) = 4.0 x 10(3) min(-1); k(cat)/K(m) = 3.3 x 10(7) min(-1) M(-1)) and acrolein (k(cat) = 2.2 x 10(2) min(-1); k(cat)/K(m) = 1.5 x 10(6) min(-1) M(-1)) and common industrial compounds such as ethyl vinyl ketone (k(cat) = 9.6 x 10(3) min(-1); k(cat)/K(m) = 8.8 x 10(7) min(-1) M(-1)) and 15-oxoprostaglandin E1 (k(cat) = 2.4 x 10(3) min(-1); k(cat)/K(m) = 2.4 x 10(9) min(-1) M(-1)). Furthermore, transfection of human embryonic kidney cells with a rat liver AO expression vector protected these cells from challenge with HNE. The concentration of HNE at which 50% of the cells were killed after 24 h increased from approximately 15 microM in control cells to approximately 70 microM in AO-transfected cells. Overexpression of AO also completely abolished protein alkylation by HNE at all concentrations tested (up to 30 microM). Thus, we describe a novel antioxidative activity of a previously characterized bioactive lipid-metabolizing enzyme that could prove to be therapeutically or prophylactically useful due to its high catalytic rate and inducibility.  (+info)

Immunohistochemical localization of guinea-pig leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin 13-reductase. (3/10)

We have cloned cDNA for leukotriene B4 12-hydroxydehydrogenase (LTB4 12-HD)/15-ketoprostaglandin 13-reductase (PGR) from guinea-pig liver. LTB4 12-HD catalyzes the conversion of LTB4 into 12-keto-LTB4 in the presence of NADP+, and plays an important role in inactivating LTB4. The cDNA contained an ORF of 987 bp that encodes a protein of 329 amino-acid residues with a 78% identity with porcine LTB4 12-HD. The amino acids in the putative NAD+/NADP+ binding domain are well conserved among the pig, guinea-pig, human, rat, and rabbit enzymes. The guinea-pig LTB4 12-HD (gpLTB4 12-HD) was expressed as a glutathione S-transferase (GST) fusion protein in Escherichia coli, which exhibited similar enzyme activities to porcine LTB4 12-HD. We examined the 15-ketoprostaglandin 13-reductase (PGR) activity of recombinant gpLTB4 12-HD, and confirmed that the Kcat of the PGR activity is higher than that of LTB4 12-HD activity by 200-fold. Northern and Western blot analyses revealed that gpLTB4 12-HD/PGR is widely expressed in guinea-pig tissues such as liver, kidney, small intestine, spleen, and stomach. We carried out immunohistochemical analyses of this enzyme in various guinea-pig tissues. Epithelial cells of calyx and collecting tubules in kidney, epithelial cells of airway, alveoli, epithelial cells in small intestine and stomach, and hepatocytes were found to express the enzyme. These findings will lead to the identification of the unrevealed roles of PGs and LTs in these tissues.  (+info)

Structural basis of leukotriene B4 12-hydroxydehydrogenase/15-Oxo-prostaglandin 13-reductase catalytic mechanism and a possible Src homology 3 domain binding loop. (4/10)

The bifunctional leukotriene B(4) 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (LTB(4) 12-HD/PGR) is an essential enzyme for eicosanoid inactivation. It is involved in the metabolism of the E and F series of 15-oxo-prostaglandins (15-oxo-PGs), leukotriene B(4) (LTB(4)), and 15-oxo-lipoxin A(4) (15-oxo-LXA(4)). Some nonsteroidal anti-inflammatory drugs (NSAIDs), which primarily act as cyclooxygenase inhibitors also inhibit LTB(4) 12-HD/PGR activity. Here we report the crystal structure of the LTB(4) 12-HD/PGR, the binary complex structure with NADP(+), and the ternary complex structure with NADP(+) and 15-oxo-PGE(2). In the ternary complex, both in the crystalline form and in solution, the enolate anion intermediate accumulates as a brown chromophore. PGE(2) contains two chains, but only the omega-chain of 15-oxo-PGE(2) was defined in the electron density map in the ternary complex structure. The omega-chain was identified at the hydrophobic pore on the dimer interface. The structure showed that the 15-oxo group forms hydrogen bonds with the 2'-hydroxyl group of nicotine amide ribose of NADP(+) and a bound water molecule to stabilize the enolate intermediate during the reductase reaction. The electron-deficient C13 atom of the conjugated enolate may be directly attacked by a hydride from the NADPH nicotine amide in a stereospecific manner. The moderate recognition of 15-oxo-PGE(2) is consistent with a broad substrate specificity of LTB(4) 12-HD/PGR. The structure also implies that a Src homology domain 3 may interact with the left-handed proline-rich helix at the dimer interface and regulate LTB(4) 12-HD/PGR activity by disruption of the substrate binding pore to accommodate the omega-chain.  (+info)

Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation. (5/10)

This report identifies a novel gene encoding 15-oxoprostaglandin-Delta13-reductase (PGR-2), which catalyzes the reaction converting 15-keto-PGE2 to 13,14-dihydro-15-keto-PGE2. The expression of PGR-2 is up-regulated in the late phase of 3T3-L1 adipocyte differentiation and predominantly distributed in adipose tissue. Overexpression of PGR-2 in cells decreases peroxisome proliferator-activated receptor gamma (PPARgamma)-dependent transcription and prohibits 3T3-L1 adipocyte differentiation without affecting expression of PPARgamma. Interestingly, we found that 15-keto-PGE2 can act as a ligand of PPARgamma to increase co-activator recruitment, thus activating PPARgamma-mediated transcription and enhancing adipogenesis of 3T3-L1 cells. Overexpression of 15-hydroxyprostaglandin dehydrogenase, which catalyzes the oxidation reaction of PGE2 to form 15-keto-PGE2, significantly increased PPARgamma-mediated transcription in a PGE2-dependent manner. Reciprocally, overexpression of wild-type PGR-2, but not the catalytically defective mutant, abolished the effect of 15-keto-PGE2 on PPARgamma activation. These results demonstrate a novel link between catabolism of PGE2 and regulation of ligand-induced PPARgamma activation.  (+info)

Restoration of leukotriene B(4)-12-hydroxydehydrogenase/15- oxo-prostaglandin 13-reductase (LTBDH/PGR) expression inhibits lung cancer growth in vitro and in vivo. (6/10)

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Up-regulation of human prostaglandin reductase 1 improves the efficacy of hydroxymethylacylfulvene, an antitumor chemotherapeutic agent. (7/10)

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Inhibition of pulmonary prostaglandin metabolism by exposure of animals to oxygen or nitrogen dioxide. (8/10)

The effects of exposure of animals to 100% O2 and NO2 on the rate of prostaglandin metabolism by lung and kidney were studied in vitro. Exposure of guinea pigs to 100% O2 for 48 h inhibited the metabolism of prostaglandin F2 alpha by both NAD+- and NADP+-dependent prostaglandin dehydrogenase in lung, but had no effect on the metabolism in kidney. Succinate dehydrogenase, but not glucose 6-phosphate dehydrogenase, in guinea-pig lung was inhibited by exposure to 100% O2. Exposure to 46 p.p.m. but not 16 or 29 p.p.m. NO2 for 6 h inhibited guinea-pig lung prostaglandin dehydrogenase in vitro. The inhibition of pulmonary prostaglandin dehydrogenase by exposure to 100% O2 or to 49 p.p.m. NO2 was dependent on the duration of exposure, but returned to control values within 7 days after cessation of the exposure. The pulmonary transport system responsible for removing circulating prostaglandins from the blood was not affected by exposure to 100% O2 as measured by using the isolated perfused lung. Kinetic analysis of the inhibition of pulmonary prostaglandin dehydrogenase activity in guinea pig exposed to 100% O2 showed non-competitive inhibition with respect to both prostaglandin F2 alpha and NAD+, which suggests destruction or inactivation of the enzyme. Pulmonary prostaglandin dehydrogenase appears to be inhibited by exposure to oxidant gases, which may lead to elevated prostaglandin concentrations in the lungs or in the systemic circulation.  (+info)

Catalysis of the reaction: 15-keto-prostaglandin + NAD(P)H + H+ -> 13,14-dihydro-15-keto-prostaglandin + NAD(P)+. This reaction is the reduction of 15-keto-prostaglandin. [EC:1.3.1.48, GOC:mw, KEGG:R04556, KEGG:R04557, PMID:17449869]
Complete information for PTGR1 gene (Protein Coding), Prostaglandin Reductase 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Adaikan, G P., Tai, M.Y., Lau, L.C. (1984). A comparison of some pharmacological actions of prostaglandin E1, 6-oxo-PGE1and PGI2. Prostaglandins 27 (4) : 505-516. [email protected] Repository. https://doi.org/10.1016/0090-6980(84)90086- ...
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Define mantis: any of an order or suborder (Mantodea and especially family Mantidae) of large usually green insects that feed on other insects and…
Advanced targeted therapies have the potential to slow lung cancer growth and improve patient outcomes. according to a series of studies presented at the 13th World Conference on Lung Cancer
5185 The potent induction of apoptosis in various types of tumor cells, contrasted to largely reversible non-apoptotic effects in normal cells is one of the hallmarks of a novel dual-action DNA- and protein-alkylating drug, irofulven (hydroxymethylacylfulvene). Previous studies suggested the contribution of both nuclear and cytoplasmic signaling to apoptosis by irofulven. To better understand the nature of the differential responses of tumor and normal cells, we used a cell-free apoptosis mimicking system consisting of isolated nuclei and cytosolic extracts. We examined homologous and heterologous mixes of nuclei and cytosols from cancer cells (CEM and LNCaP-Pro5), which initiate apoptosis in response to irofulven, and from normal cells (NCM 460), which display a non-apoptotic cytostatic response. Cytosols were obtained either from untreated or drug-treated cells. In the latter case, irofulven concentrations were adjusted to provide equal levels of intracellular drug-macromolecule adducts in ...
MP Biomedicals Solid Sample Instrument Platform contains all of the instrumentation necessary to run solid samples using MP Biomedicals Peroxide or Alkenal Test Kit. This Solid Sample Instrument Platform requires the use of MP Biomedicals Solid Sample Consumables Platform (Item Number |b|07SC2000|/b|).|br|Each Solid Sample Instrument Platform contains the following items: timer, two block dri-bath 110V, 12.75mm dri-block insert, vortex 110V, 13mm test tube rack, 10-100µL pipette PDP, vacuum pump assembly 110V, acrylic base, 100-1000µL pipette PDP, 15mm dri-block insert, 20mm test tube rack, SafTest analyzer, tube rocker 110V, O-ring for vacuum pump assembly.|/br|
Human ζ-crystallin is a Zn(2+)-lacking medium-chain dehydrogenase/reductase (MDR) included in the quinone oxidoreductase (QOR) family because of its activity with quinones. In the present work a novel enzymatic activity was characterized: the double bond α,β-hydrogenation of medium-chain 2-alkenals and 3-alkenones. The enzyme is especially active with lipid peroxidation products such as 4-hydroxyhexenal, and a role in their detoxification is discussed. This specificity is novel in the QOR family, and it is similar to that described in the distantly related alkenal/one reductase family. Moreover, we report the X-ray structure of ζ-crystallin, which represents the first structure solved for a tetrameric Zn(2+)-lacking MDR, and which allowed the identification of the active-site lining residues. Docking simulations suggest a role for Tyr53 and Tyr59 in catalysis. The kinetics of Tyr53Phe and Tyr59Phe mutants support the implication of Tyr53 in binding/catalysis of alkenal/one substrates, while Tyr59 is
Urine Meaning with Examples - In mammals, a fluid excretion from the kidneys; in birds and reptiles, a solid or semisolid excretion.; To urinate.;
Estrogen, long known to fuel breast cancer growth, may spur lung cancer development as well, according to University of Pittsburgh findings presented April 2 at the American Association of Cancer Research meeting. The results are the first directly demonstrating increased growth of lung cancer in the presence of estrogen.
https://doi.org/10.18632/oncotarget.8081 Zsuzsanna Nemeth, Eva Csizmadia, Lisa Vikstrom, Mailin Li, Kavita Bisht, Alborz Feizi, Sherrie Otterbein, Brian Zuckerbraun, Daniel B. Costa, Pier Paolo...
1. We studied the effect of oral administration of acetylsalicylic acid (1200 mg/day for 3 days) on the urinary excretion of 6-ketoprostaglandin F1α in normal human subjects as an index of prostacyclin production in vivo.. 2. The concentrations and excretion rate in urine fell to 45% of pretreatment levels in 3 days, but returned to pretreatment values after 7 days.. 3. These results suggest that production of prostacyclin in vivo is only partially inhibited by high doses of aspirin and that there are sites of production of prostacyclin which are protected from inhibition by aspirin and which contribute to urinary 6-ketoprostaglandin F1α. The measurement of 6-ketoprostaglandin F1α in urine may therefore be of only limited value as an index of the metabolism of vascular tissue in vivo.. ...
Sigma-Aldrich offers abstracts and full-text articles by [T W Moody, F Zia, M Draoui, D E Brenneman, M Fridkin, A Davidson, I Gozes].
NADP-dependent oxidoreductase, putative; FUNCTIONS IN: oxidoreductase activity, binding, catalytic activity, zinc ion binding; INVOLVED IN: response to oxidative stress, response to cyclopentenone; EXPRESSED IN: 11 plant structures; EXPRESSED DURING: LP.06 six leaves visible, 4 anthesis, LP.04 four leaves visible, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: GroES-like (InterPro:IPR011032), NAD(P)-binding (InterPro:IPR016040), Alcohol dehydrogenase, zinc-binding (InterPro:IPR013149), Alcohol dehydrogenase superfamily, zinc-containing (InterPro:IPR002085); BEST Arabidopsis thaliana protein match is: AT-AER (alkenal reductase); 2-alkenal reductase (TAIR:AT5G16970.1); Has 10893 Blast hits to 10880 proteins in 1040 species: Archae - 76; Bacteria - 5027; Metazoa - 696; Fungi - 750; Plants - 299; Viruses - 0; Other Eukaryotes - 4045 (source: NCBI BLink ...
Glutathione is at present one of many most analyzed antioxidants. This is likely because of it being endogenously synthesized all all through the body and it is basically located in all cells, at times in fairly high concentrations. Investigations have highlighted several roles in which it is used which includes antioxidant protection, detoxification of electrophilic xenobiotics, modulation of redox controlled sign transduction, storage and transportation of cysteine, regulation of mobile proliferation, synthesis of deoxyribonucleotide synthesis, regulation of immune responses, and regulation of leukotriene and prostaglandin metabolism[thirteen ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Weve reviewed the documented benefits of curcurmin - the bioactive ingredient in turmeric - extensively on this blog, yet it seems that, with each new day, researchers from around the world are unearthing yet more immune-boosting applications for this natural compound. Past research has already indicated that curcumin supplements may prevent the spread of certain cancers including colon and prostate. Now, a new study from the General Hospital of Chinese PLA has indicated that this substance might help manage lung cancer as well.. The American Cancer Society estimates that 228,190 Americans are diagnosed with lung cancer each year, with smokers facing a much higher risk of developing the disease. The source notes that lung cancer has a higher mortality rate than any other form of the condition.. According to a study abstract released by Pubmed, the scientists projected that curcumin may be able to restrict the growth and metastasis of lung cancer cells based on evidence of such behavior with ...
Žmogaus papilomos viruso (ŽPV) ginekologiškai aukštos rizikos genotipų (16,18,26,31,33,35,39,45,51,52,53,56,58,59,66,68,69,73,82) nustatymas PGR ...
Other names in common use include prostaglandin-D2 11-reductase, reductase, 15-hydroxy-11-oxoprostaglandin, PGD2 11- ... prostaglandin-D2 11-reductase, PGF synthetase, NADPH-dependent prostaglandin D2 11-keto reductase, and prostaglandin 11-keto ... Reingold DF, Kawasaki A, Needleman P (1981). "A novel prostaglandin 11-keto reductase found in rabbit liver". Biochim. Biophys ... Wong PY (1981). "Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver". Biochim. ...
In enzymology, a 2-alkenal reductase (EC 1.3.1.74) is an enzyme that catalyzes the chemical reaction n-alkanal + NAD(P)+ ⇌ {\ ... A new role for leukotriene B4 12-hydroxydehydrogenase/15-oxoprostaglandin 13-reductase". J. Biol. Chem. 276 (44): 40803-10. doi ...
... prostaglandin Delta13-reductase, prostaglandin 13-reductase, and 15-ketoprostaglandin Delta13-reductase. As of late 2007, 4 ... In enzymology, a 15-oxoprostaglandin 13-oxidase (EC 1.3.1.48) is an enzyme that catalyzes the chemical reaction (5Z)-(15S)- ... The systematic name of this enzyme class is (5Z)-(15S)-11alpha-hydroxy-9,15-dioxoprostanoate:NAD(P)+ Delta13-oxidoreductase. ... Hansen HS (1982). "Purification and assay of 15-ketoprostaglandin delta 13-reductase from bovine lung". Methods Enzymol. 86: ...
9-keto-prostaglandin E2 reductase, 9-ketoprostaglandin reductase, PGE-9-ketoreductase, PGE2 9-oxoreductase, PGE2 reductase-9- ... In enzymology, a prostaglandin-E2 9-reductase (EC 1.1.1.189) is an enzyme that catalyzes the chemical reaction (5Z,13E)-(15S)- ... Watkins JD, Jarabak J (1985). "The effect of NaCl intake on 9-ketoprostaglandin reductase activity in the rabbit kidney". ... Schlegel W, Kruger S, Korte K (1984). "Purification of prostaglandin E2 reductase-9-oxoreductase from human decidua vera". FEBS ...
L-xylulose reductase Ja 1.1.1.11 D-arabitol + NAD+ ⇌. {\displaystyle \rightleftharpoons }. D-xylulose + NADH + H+ D-arabitol 4- ... D-xylulose reductase 1.1.1.10 Xylitol + NADP+ ⇌. {\displaystyle \rightleftharpoons }. L-xylulose + NADPH + H+ ...
... gmp reductase MeSH D08.811.682.655.500 --- nitrate reductases MeSH D08.811.682.655.500.124 --- nitrate reductase MeSH D08.811. ... 682.655.500.200 --- nitrate reductase (nadh) MeSH D08.811.682.655.500.249 --- nitrate reductase (nad(p)h) MeSH D08.811.682.655. ... reductase (nadh) MeSH D08.811.682.660.390 --- enoyl-(acyl-carrier protein) reductase (nadph, b-specific) MeSH D08.811.682.660. ... ferredoxin-nitrite reductase MeSH D08.811.682.655.750.500 --- nitrite reductase (NAD(P)H) MeSH D08.811.682.657.163 --- aldehyde ...
... flavin reductase (NADH) EC 1.5.1.37: FAD reductase (NADH) EC 1.5.1.38: FMN reductase (NADPH) EC 1.5.1.39: FMN reductase (NAD(P) ... zeatin reductase EC 1.3.1.70: D14-sterol reductase EC 1.3.1.71: D24(241)-sterol reductase EC 1.3.1.72: D24-sterol reductase EC ... nitrite reductase (NAD(P)H) EC 1.7.1.5: hyponitrite reductase EC 1.7.1.6: azobenzene reductase EC 1.7.1.7: GMP reductase EC 1.7 ... reductase EC 1.3.1.93: very-long-chain enoyl-CoA reductase EC 1.3.1.94: polyprenol reductase EC 1.3.1.95: acrylyl-CoA reductase ...
The chlorobenzene ring is buried in the hydrophobic pore used as a binding site by the omega-chain of 15-oxo-PGE2. The carboxyl ... The crystal structure of the ternary complex of leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin (15-oxo-PG) 13- ... reductase (LTB4 12HD/PGR), an essential enzyme for eicosanoid inactivation pathways, with indomethacin and NADP+ has been solve ... The crystal structure of the ternary complex of leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin (15-oxo-PG) 13- ...
... prostaglandin Delta13-reductase, prostaglandin 13-reductase, and 15-ketoprostaglandin Delta13-reductase. As of late 2007, 4 ... In enzymology, a 15-oxoprostaglandin 13-oxidase (EC 1.3.1.48) is an enzyme that catalyzes the chemical reaction (5Z)-(15S)- ... The systematic name of this enzyme class is (5Z)-(15S)-11alpha-hydroxy-9,15-dioxoprostanoate:NAD(P)+ Delta13-oxidoreductase. ... Hansen HS (1982). "Purification and assay of 15-ketoprostaglandin delta 13-reductase from bovine lung". Methods Enzymol. 86: ...
In enzymology, a 2-alkenal reductase (EC 1.3.1.74) is an enzyme that catalyzes the chemical reaction n-alkanal + NAD(P)+ ⇌ {\ ... A new role for leukotriene B4 12-hydroxydehydrogenase/15-oxoprostaglandin 13-reductase". J. Biol. Chem. 276 (44): 40803-10. doi ...
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity ... Prostaglandin reductase 1Add BLAST. 329. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view ... Cluster: Prostaglandin reductase 1. 25. Q14914-2. UPI000053E997. UPI0001F55035. UPI0005D0077E. A0A2I3S9F2. UPI0007DB8DD4. ... Cluster: Prostaglandin reductase 1. 42. Q14914-2. UPI000053E997. UPI0001F55035. UPI0005D0077E. A0A2I3S9F2. UPI0007DB8DD4. ...
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2- ... Prostaglandin reductase 2. *PTGR 2. *PTGR2. *PTGR2_HUMAN. *Zinc binding alcohol dehydrogenase domain containing 1 ... alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits ...
Prostaglandin Reductase 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human ... PTGR1 (Prostaglandin Reductase 1) is a Protein Coding gene. Among its related pathways are Arachidonic acid metabolism and ... Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of ... XIII:10198-11295. -- 33 Thale Cress. (Arabidopsis thaliana). eudicotyledons. AT3G03080 30 *49.17 (n) ...
13,14-dihydro-15-keto-prostaglandin + NAD(P)+. This reaction is the reduction of 15-keto-prostaglandin. [EC:1.3.1.48, GOC:mw, ... Known as: prostaglandin delta13-reductase activity, 15-oxoprostaglandin 13-reductase activity, 15-oxo-delta13-prostaglandin ... reductase activity Expand. Catalysis of the reaction: 15-keto-prostaglandin + NAD(P)H + H+ -, 13,14-dihydro-15-keto- ... The occurrence of three enzymes of prostaglandin metabolism, 15-hydroxy-prostaglandin-dehydrogenase, delta13 -reductase and… ...
... the aldo-keto reductases and the short-chain dehydrogenases/reductases (Oppermann, 2007). It is worthwhile to further ... Thirteen metabolites were detected and identified, seven of which were purified from fecal samples collected from [6]-shogaol- ... shogaol to generate M11 and whether aldo-keto reductases and/or short-chain dehydrogenases/reductases can reduce [6]-shogaol ... 2007) Carbonyl reductases: the complex relationships of mammalian carbonyl- and quinone-reducing enzymes and their role in ...
Przedborski S, Jackson-Lewis V: Mechanisms of MPTP toxicity. Mov Disord 1998, 13(1):35-38.PubMedGoogle Scholar. ... PC12 cells at passage 15-20 (ATCC, Manassas, VA) were grown in 75 cm2tissue culture flasks at 37°C under an atmosphere of 5% CO ... Within the subset of genes expressed at a 2-fold or greater level in the MPTP microarray experiments, these 13 were found co- ... A new role for leukotriene B4 12-hydroxydehydrogenase/15-oxoprostaglandin 13-reductase. J Biol Chem 2001, 276(44):40803-40810. ...
HUMAN 604296 Glyoxylate reductase/hydroxypyruvate reductase (EC 1.1.1.79) (EC 1.1.1.81) [GLXR] [MSTP035] GRIN1 9q34.3 Q05586 ... XV) chain precursor [Contains: Restin (Endostatin-XV) (Related to endostatin) (Restin-I); Restin-2 (Restin-II); Restin-3 ( ... Ferric-chelate reductase 1-like protein) [C9orf4] FSD1L 9q31 Q9BXM9 FSD1L_HUMAN 609829 FSD1-like protein (Coiled-coil domain- ... Short chain dehydrogenase/reductase family 13C member 1) [C9orf99] [SDR13C1] HSPA5 9q33-q34.1 P11021 GRP78_HUMAN 138120 78 kDa ...
L-xylulose reductase Ja 1.1.1.11 D-arabitol + NAD+ ⇌. {\displaystyle \rightleftharpoons }. D-xylulose + NADH + H+ D-arabitol 4- ... D-xylulose reductase 1.1.1.10 Xylitol + NADP+ ⇌. {\displaystyle \rightleftharpoons }. L-xylulose + NADPH + H+ ...
Am meisten referenzierte anti-Prostaglandin Reductase 1 Antikörper. Show all anti-Prostaglandin Reductase 1 (PTGR1) Antikörper ... Weitere Antikörper gegen Prostaglandin Reductase 1 Interaktionspartner. Human Prostaglandin Reductase 1 (PTGR1) ... Prostaglandin Reductase 1 (PTGR1) Antigen-Profil Protein Überblick This gene encodes an enzyme that is involved in the ... Top anti-Prostaglandin Reductase 1 Antikörper auf antikoerper-online.de. Showing 10 out of 50 products:. Katalog Nr.. ...
15-Oxoprostaglandin 13-Reductase (Structural Class). NDF-RT (Drug Classification). Drug Classes (NDF-RT). Details. ... 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid (Structural Class). NDF-RT (Drug Classification). Drug ... 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid. ...
Purification, cDNA cloning and expression of 15-oxoprostaglandin 13-reductase from pig lung PubMed ID:9461497. ...
Prostaglandin reductase 2 antibody. *PTGR 2 antibody. *PTGR2 antibody. *PTGR2_HUMAN antibody ... Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2- ... alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits ...
... cortisone Δ4-5β-reductase; steroid 5β-reductase; testosterone 5β-reductase; Δ4-3-ketosteroid 5β-reductase; Δ4-5β-reductase; Δ4- ... 5β-reductase; androstenedione 5β-reductase; cholestenone 5β-reductase; cortisone 5β-reductase; cortisone β-reductase; ... reductase; NADPH 2-enoyl Co A reductase; enoyl-ACp reductase; enoyl-[acyl-carrier-protein] reductase (NADPH2, A-specific); acyl ... reductase; NADPH 2-enoyl Co A reductase; enoyl acyl-carrier-protein reductase (ambiguous); enoyl-ACP reductase (ambiguous); ...
... reductase 1.3.1.93 Very-long-chain enoyl-CoA reductase 1.3.1.94 Polyprenol reductase 1.3.1.95 Acryloyl-CoA reductase (NADH) 1.3 ... reductase 1.3.1.105 2-methylene-furan-3-one reductase 1.3.1.106 Cobalt-precorrin-6A reductase 1.3.1.107 Sanguinarine reductase ... sterol reductase 1.3.1.72 Delta(24)-sterol reductase 1.3.1.73 1,2-dihydrovomilenine reductase 1.3.1.74 2-alkenal reductase (NAD ... 2-enoate reductase 1.3.1.32 Maleylacetate reductase 1.3.1.33 Protochlorophyllide reductase 1.3.1.34 2,4-dienoyl-CoA reductase ( ...
2-alkenal reductase (NAD(P)(+)) ,, Acrylyl-CoA reductase (NADPH) ,, Enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific) ... D-xylulose reductase. 0. 2.952. --. DIRECT. Enzyme Commission (EC). Enoyl-[acyl-carrier-protein] reductase. 0. 2.651. --. ... INHERITED FROM: 2-alkenal reductase ,, Trans-2-enoyl-CoA reductase (NADPH) ,, 15-oxoprostaglandin 13-oxidase ,, With NAD(+) or ... ketose reductase (sorbitol dehydrogenase), formaldehyde dehydrogenase, quinone oxidoreductase and 2,4-dienoyl-CoA reductase. ...
... oxo-prostaglandin 13-reductase (LTBDH/PGR) expression inhibits lung cancer growth in vitro and in vivo. Lung Cancer 68:161-9 ... 2008) 15-Hydroxyprostaglandin dehydrogenase suppresses K-RasV12-dependent tumor formation in Nu/Nu mice. Mol Carcinog 47:466-77 ... We have also cloned and expressed the cDNA of porcine lung 13-PGR in an active form. We propose to identify the binding domains ... However, regulation of the 15-PGDH gene expression has not been explored in any detail. We have cloned the genomic DNA of mouse ...
Prostaglandin Reductase 2 (PTGR2) Polyclonal Hôte: Lapin IgG WB Reactivité: Porc ELISA Biotin IHC Reactivité: Boeuf (Vache) HRP ... anti-PTGR2 anticorps (Prostaglandin Reductase 2) (AA 72-102) Rabbit, Polyclonal, Ig Fraction. ... anti-PTGR2 anticorps (Prostaglandin Reductase 2) (AA 65-94) Rabbit, Polyclonal, Ig Fraction. ... zinc binding alcohol dehydrogenase, domain containing 1 , prostaglandin reductase 2 , Zinc-binding alcohol dehydrogenase domain ...
5,10-Methylenetetrahydrofolate Reductase (FADH2) 5,10-Methylenetetrahydrofolate-Reductase (NADH) use Methylenetetrahydrofolate ... 6-Oxoprostaglandin F1 alpha use 6-Ketoprostaglandin F1 alpha 6-Phosphofructo-2-kinase-fructose-2,6-bisphosphatase use ... 5-alpha-Reductase, Testosterone use 3-Oxo-5-alpha-Steroid 4-Dehydrogenase ... 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid ...
Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific). *Enoyl-(Acyl-Carrier-Protein) Reductase (NADH) ...
13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) = 15-oxoprostaglandin E2 + H(+) + NADH ... RHEA:11918 15-oxoprostaglandin E2 + H(+) + NADH =, 13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) ... RHEA:11917 13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) =, 15-oxoprostaglandin E2 + H(+) + NADH ... RHEA:11919 13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) ,=, 15-oxoprostaglandin E2 + H(+) + NADH ...
Aldose reductase-like) (Aldose reductase-related protein) (ARP) (hARP) (Small intestine reductase) (SI reductase). ... Ptgr1 Ltb4dh] Prostaglandin reductase 1 (PRG-1) (EC 1.3.1.-) (15-oxoprostaglandin 13-reductase) (PGR) (EC 1.3.1.48) (NADP- ... AKR1B10 AKR1B11] Aldo-keto reductase family 1 member B10 (EC 1.1.1.300) (EC 1.1.1.54) (ARL-1) ( ... 11733004] Immunohistochemical localization of guinea-pig leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin 13- ...
UDihydrofolate reductase. Not Available. Escherichia coli (strain K12). UAldo-keto reductase family 1 member C1. Not Available ... UDehydrogenase/reductase SDR family member 11. Not Available. Humans. U3-hydroxy-3-methylglutaryl-coenzyme A reductase. Not ... UProstaglandin reductase 1. Not Available. Humans. USulfite reductase [NADPH] flavoprotein alpha-component. Not Available. ... U2,4-dienoyl-CoA reductase, mitochondrial. Not Available. Humans. UOxidoreductase, short chain dehydrogenase/reductase family. ...
  • In enzymology, a 2-alkenal reductase (EC 1.3.1.74) is an enzyme that catalyzes the chemical reaction n-alkanal + NAD(P)+ ⇌ {\displaystyle \rightleftharpoons } alk-2-enal + NAD(P)H + H+ The 3 substrates of this enzyme are n-alkanal, NAD+, and NADP+, whereas its 4 products are alk-2-enal, NADH, NADPH, and H+. (wikipedia.org)
  • We propose to identify the binding domains of NADPH and 15-ketoprostaglandins of 13-PGR by photoaffinity labeling studies and to elucidate the structure and function of the enzyme by site-directed mutagenesis. (grantome.com)
  • Thirteen metabolites were detected and identified, seven of which were purified from fecal samples collected from -shogaol-treated mice. (aspetjournals.org)
  • M9 and M11 were identified as the major metabolites in four different cancer cell lines (HCT-116, HT-29, H-1299, and CL-13), and M13 was detected as a major metabolite in HCT-116 human colon cancer cells. (aspetjournals.org)
  • The latter subsequently catalyzes the reduction of 15-oxo-PGE2 to 15-ketoprostaglandin F2 alpha ( 15-Keto-PGF2alpha ) that is in turn reduced by CBR1 to PGF2 alpha . (bio-rad.com)
  • PGF2 is mainly synthesized directly from PGH2 by PGH2 9,11-endoperoxide reductase. (hmdb.ca)
  • However, regulation of the 15-PGDH gene expression has not been explored in any detail. (grantome.com)
  • We have cloned the genomic DNA of mouse 15-PGDH gene and have sequenced the 5? (grantome.com)
  • We plan to identify specific regulatory elements involved in gene expression, induced by PMA and dexamethosone and to elucidate the signal transduction pathway of androgen activated 15-PGDH expression. (grantome.com)
  • 15-Keto-prostaglandin F2a is further metabolized by its delta13-reduction, beta-oxidation and omega oxidation. (hmdb.ca)
  • 11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P) + = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H. (uniprot.org)
  • Cloning, characterization and structure and activity relationship at 15-PGDH have been extensively studied. (grantome.com)