Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Reference Standards: A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Linear Energy Transfer: Rate of energy dissipation along the path of charged particles. In radiobiology and health physics, exposure is measured in kiloelectron volts per micrometer of tissue (keV/micrometer T).Reference Values: The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Electrophoresis, Starch Gel: Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.Creutzfeldt-Jakob Syndrome: A rare transmissible encephalopathy most prevalent between the ages of 50 and 70 years. Affected individuals may present with sleep disturbances, personality changes, ATAXIA; APHASIA, visual loss, weakness, muscle atrophy, MYOCLONUS, progressive dementia, and death within one year of disease onset. A familial form exhibiting autosomal dominant inheritance and a new variant CJD (potentially associated with ENCEPHALOPATHY, BOVINE SPONGIFORM) have been described. Pathological features include prominent cerebellar and cerebral cortical spongiform degeneration and the presence of PRIONS. (From N Engl J Med, 1998 Dec 31;339(27))Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Chromatography, DEAE-Cellulose: A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Longevity: The normal length of time of an organism's life.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).ADP Ribose Transferases: Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.PhosphopeptidesBacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Adenosine Diphosphate Ribose: An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Plant Root Nodulation: The formation of a nitrogen-fixing cell mass on PLANT ROOTS following symbiotic infection by nitrogen-fixing bacteria such as RHIZOBIUM or FRANKIA.Schistosoma japonicum: A species of trematode blood flukes belonging to the family Schistosomatidae whose distribution is confined to areas of the Far East. The intermediate host is a snail. It occurs in man and other mammals.Bradyrhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria usually containing granules of poly-beta-hydroxybutyrate. They characteristically invade the root hairs of leguminous plants and act as intracellular symbionts.Schistosomiasis japonica: Schistosomiasis caused by Schistosoma japonicum. It is endemic in the Far East and affects the bowel, liver, and spleen.Apyrase: A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC 3.6.1.5.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Stroke: A group of pathological conditions characterized by sudden, non-convulsive loss of neurological function due to BRAIN ISCHEMIA or INTRACRANIAL HEMORRHAGES. Stroke is classified by the type of tissue NECROSIS, such as the anatomic location, vasculature involved, etiology, age of the affected individual, and hemorrhagic vs. non-hemorrhagic nature. (From Adams et al., Principles of Neurology, 6th ed, pp777-810)Astrocytes: A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.Brain Ischemia: Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.ReadingUp-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Cerebral Infarction: The formation of an area of NECROSIS in the CEREBRUM caused by an insufficiency of arterial or venous blood flow. Infarcts of the cerebrum are generally classified by hemisphere (i.e., left vs. right), lobe (e.g., frontal lobe infarction), arterial distribution (e.g., INFARCTION, ANTERIOR CEREBRAL ARTERY), and etiology (e.g., embolic infarction).

An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast. (1/1483)

The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well.  (+info)

Cell adhesion regulates the interaction between the docking protein p130(Cas) and the 14-3-3 proteins. (2/1483)

Integrin ligand binding induces a signaling complex formation via the direct association of the docking protein p130(Cas) (Cas) with diverse molecules. We report here that the 14-3-3zeta protein interacts with Cas in the yeast two-hybrid assay. We also found that the two proteins associate in mammalian cells and that this interaction takes place in a phosphoserine-dependent manner, because treatment of Cas with a serine phosphatase greatly reduced its ability to bind 14-3-3zeta. Furthermore, the Cas-14-3-3zeta interaction was found to be regulated by integrin-mediated cell adhesion. Thus, when cells are detached from the extracellular matrix, the binding of Cas to 14-3-3zeta is greatly diminished, whereas replating the cells onto fibronectin rapidly induces the association. Consistent with these results, we found that the subcellular localization of Cas and 14-3-3 is also regulated by integrin ligand binding and that the two proteins display a significant co-localization during cell attachment to the extracellular matrix. In conclusion, our results demonstrate that 14-3-3 proteins participate in integrin-activated signaling pathways through their interaction with Cas, which, in turn, may contribute to important biological responses regulated by cell adhesion to the extracellular matrix.  (+info)

Protein kinase C mu is negatively regulated by 14-3-3 signal transduction proteins. (3/1483)

Recent studies have documented direct interaction between 14-3-3 proteins and key molecules in signal transduction pathways like Ras, Cbl, and protein kinases. In T cells, the 14-3-3tau isoform has been shown to associate with protein kinase C theta and to negatively regulate interleukin-2 secretion. Here we present data that 14-3-3tau interacts with protein kinase C mu (PKCmu), a subtype that differs from other PKC members in structure and activation mechanisms. Specific interaction of PKCmu and 14-3-3tau can be shown in the T cell line Jurkat by immunocoprecipitiation and by pulldown assays of either endogenous or overexpressed proteins using PKCmu-specific antibodies and GST-14-3-3 fusion proteins, respectively. Using PKCmu deletion mutants, the 14-3-3tau binding region is mapped within the regulatory C1 domain. Binding of 14-3-3tau to PKCmu is significantly enhanced upon phorbol ester stimulation of PKCmu kinase activity in Jurkat cells and occurs via a Cbl-like serine containing consensus motif. However, 14-3-3tau is not a substrate of PKCmu. In contrast 14-3-3tau strongly down-regulates PKCmu kinase activity in vitro. Moreover, overexpression of 14-3-3tau significantly reduced phorbol ester induced activation of PKCmu kinase activity in intact cells. We therefore conclude that 14-3-3tau is a negative regulator of PKCmu in T cells.  (+info)

Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. (4/1483)

Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/ threonine kinase Akt, which then phosphorylates and inactivates components of the apoptotic machinery, including BAD and Caspase 9. In this study, we demonstrate that Akt also regulates the activity of FKHRL1, a member of the Forkhead family of transcription factors. In the presence of survival factors, Akt phosphorylates FKHRL1, leading to FKHRL1's association with 14-3-3 proteins and FKHRL1's retention in the cytoplasm. Survival factor withdrawal leads to FKHRL1 dephosphorylation, nuclear translocation, and target gene activation. Within the nucleus, FKHRL1 triggers apoptosis most likely by inducing the expression of genes that are critical for cell death, such as the Fas ligand gene.  (+info)

Ca2+-induced apoptosis through calcineurin dephosphorylation of BAD. (5/1483)

The Ca2+-activated protein phosphatase calcineurin induces apoptosis, but the mechanism is unknown. Calcineurin was found to dephosphorylate BAD, a pro-apoptotic member of the Bcl-2 family, thus enhancing BAD heterodimerization with Bcl-xL and promoting apoptosis. The Ca2+-induced dephosphorylation of BAD correlated with its dissociation from 14-3-3 in the cytosol and translocation to mitochondria where Bcl-xL resides. In hippocampal neurons, L-glutamate, an inducer of Ca2+ influx and calcineurin activation, triggered mitochondrial targeting of BAD and apoptosis, which were both suppressible by coexpression of a dominant-inhibitory mutant of calcineurin or pharmacological inhibitors of this phosphatase. Thus, a Ca2+-inducible mechanism for apoptosis induction operates by regulating BAD phosphorylation and localization in cells.  (+info)

Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants. (6/1483)

Fusicoccin (FC) is a fungal toxin that activates the plant plasma membrane H+-ATPase by binding with 14-3-3 proteins, causing membrane hyperpolarization. Here we report on the effect of FC on a gene-for-gene pathogen-resistance response and show that FC application induces the expression of several genes involved in plant responses to pathogens. Ten members of the FC-binding 14-3-3 protein gene family were isolated from tomato (Lycopersicon esculentum) to characterize their role in defense responses. Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. Different specific subsets of 14-3-3 genes were induced after treatment with FC and during a gene-for-gene resistance response. Possible roles for the H+-ATPase and 14-3-3 proteins in responses to pathogens are discussed.  (+info)

Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import. (7/1483)

Cdc2-cyclin B1 in the G2-arrested Xenopus oocyte is held inactive by phosphorylation of Cdc2 at two negative regulatory sites, Thr14 and Tyr15. Upon treatment with progesterone, these sites are dephosphorylated by the dual specificity phosphatase, Cdc25, leading to Cdc2-cyclin B1 activation. Whereas maintenance of the G2 arrest depends upon preventing Cdc25-induced Cdc2 dephosphorylation, the mechanisms responsible for keeping Cdc25 in check in these cells have not yet been described. Here we report that Cdc25 in the G2-arrested oocyte is bound to 14-3-3 proteins and that progesterone treatment abrogates this binding. We demonstrate that Cdc25, apparently statically localized in the cytoplasm, is actually capable of shuttling in and out of the oocyte nucleus. Binding of 14-3-3 protein markedly reduces the nuclear import rate of Cdc25, allowing nuclear export mediated by a nuclear export sequence present in the N-terminus of Cdc25 to predominate. If 14-3-3 binding to Cdc25 is prevented while nuclear export is inhibited, the coordinate nuclear accumulation of Cdc25 and Cdc2-cyclin B1 facilitates their mutual activation, thereby promoting oocyte maturation.  (+info)

The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2-adrenergic receptor subtypes. (8/1483)

The alpha2-adrenergic receptors (alpha2ARs) are localized to and function on the basolateral surface in polarized renal epithelial cells via a mechanism involving the third cytoplasmic loop. To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. A protein doublet of approximately 30 kDa in Madin-Darby canine kidney cells or pig brain cytosol (alpha2B >/= alpha2C>> alpha2A) was identified. The interacting protein was purified by sequential DEAE and size exclusion chromatography, and subsequent microsequencing revealed that they are the zeta isoform of 14-3-3 proteins. [35S]Met-14-3-3zeta binds to all three native alpha2AR subtypes, assessed using a solid phase binding assay (alpha2A>/=alpha2B> alpha2C), and this binding depends on the presence of the 3i loops. Attenuation of the alpha2AR-14-3-3 interactions in the presence of a phosphorylated Raf-1 peptide corresponding to its 14-3-3 interacting domain (residues 251-266), but not by its non-phosphorylated counterpart, provides evidence for the functional specificity of these interactions and suggests one potential interface for the alpha2AR and 14-3-3 interactions. These studies represent the first evidence for G protein-coupled receptor interactions with 14-3-3 proteins and may provide a mechanism for receptor localization and/or coordination of signal transduction.  (+info)

References for Abcams Recombinant Human CD3 zeta protein (ab112263). Please let us know if you have used this product in your publication
The yeast 14-3-3 proteins, Bmh1 and Bmh2, have been implicated in many cellular processes. Our studies demonstrate that yeast 14-3-3 proteins also play important roles in longevity regulation and stress response. In this study, 14-3-3 proteins were identified as longevity factors because their overexpressions extended cell survival in an accelerated cell death assay (Figure 1A) and extended CLS (Figure 1B). Interestingly, we discovered that Bmh1 deletion also extended CLS (Figure 1C). Since Bmh1-oe extended CLS only under certain growth conditions whereas bmh1Δ extended CLS regardless of growth conditions, bmh1Δ was more likely to function in a conserved pathway to extend CLS. Therefore, we focused on determining the mechanisms underlying bmh1Δ-induced CLS extension. We showed that the bmh1Δ mutant was more resistant to challenges of heat shock (Figure 2A) and oxidative stress-inducing reagents (Figure 2, E and F). Deleting Bmh1 also increased HSE-mediated transcription (Figure 2C). In ...
Complete information for YWHAH gene (Protein Coding), Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Eta, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
YWHAH protein, antibody, gene cDNA and expression plasmid. Buy premium quality reagents at up to 60% cost saving. Bulks in stock.
The alpha2-adrenergic receptors (alpha2ARs) are localized to and function on the basolateral surface in polarized renal epithelial cells via a mechanism involving the third cytoplasmic loop. To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. A protein doublet of approximately 30 kDa in Madin-Darby canine kidney cells or pig brain cytosol (alpha2B |/= alpha2C| alpha2A) was identified.
14-3-3 epsilon/YWHAE ELISA Kits & ELISA Pair Sets are produced in house. Buy premium quality reagents at up to 60% cost saving. Bulks in stock.
Mouse 14-3-3 epsilon / YWHAE Protein (GST Tag), expressed in . Produced and quality controlled in house. High quality guaranteed. Save up to 60%. Bulk in stock.
The recombinant human YWHAE/GST chimera consists of 487 amino acids and has a predicted molecular mass of 56 kDa. It migrates as an approxiamtely 50 kDa band in SDS-PAGE under reducing conditions ...
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J:189468 Lenoir O, Flosseau K, Ma FX, Blondeau B, Mai A, Bassel-Duby R, Ravassard P, Olson EN, Haumaitre C, Scharfmann R, Specific control of pancreatic endocrine beta- and delta-cell mass by class IIa histone deacetylases HDAC4, HDAC5, and HDAC9. Diabetes. 2011 Nov;60(11):2861-71 ...
Ywhaz (untagged) - Mouse tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (Ywhaz), (10ug), 10 µg.
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Our results identified a novel pathway of YWHAZ-β-catenin axis in NSCLC cell malignancy. We showed that the metastatic activity of YWHAZ is mediated through the prevention of β-catenin ubiquitination. We showed that YWHAZ associates with phospho-β-catenin at Ser552 to retard β-catenin degradation. Subsequently, β-catenin accumulation in cancer cells contributes to its nuclear translocation and the activation of EMT-related genes (Fig. 6E). Conversely, disruption of the YWHAZ-β-catenin interaction enhances β-catenin ubiquitination and reduces cell invasiveness.. YWHAZ protein expression is well known to be associated with advanced disease grade and poor clinical outcome in Western NSCLC patients (18), but the molecular contribution of YWHAZ in lung cancer metastasis is still unknown. Particularly, it is unclear whether YWHAZ gene amplification is relevant to lung cancer progression. Here, we conducted array CGH and microarray to identify YWHAZ as an invasion-enhancer candidate. Actually, ...
Mouse monoclonal antibody raised against full length recombinant YWHAE. Recombinant protein corresponding to full length human YWHAE. (MAB1125) - Products - Abnova
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Complete information for YWHAE gene (Protein Coding), Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Epsilon, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Ywhae knockout mouse is useful for pathophysiological analyses of neuropsychiatric disorders caused by defects during neurodevelopment ...
Associated to a variety over a number field $K$, one has a family of "Hasse-Weil" L-functions, which can be combined (as an alternating product) to give the Hasse--Weil zeta function of the variety, which is comparible to the (Hasse) zeta function of a proper, regular model the variety over $\mathcal{O}_K$. I will refer to the Hasse-Weil L-functions appearing in the factorization of the zeta function as L-factors of the zeta function. For example, when all the special fibres are smooth, the two zeta functions are equal. In general they disagree at finitely many (bad) primes. One is interested in basic analytic questions, such as meromorphic continuation and functional equation.. One way in which these questions can be addressed is by proving each Hasse-Weil L-factor is automorphic. According to Langlands in the short article "where stands functoriality today?", there are some deficiencies with this strategy (see, for example, his remarks in section $6$). Bearing in mind the difficulty of these ...
Competence is comfortable - but maybe it shouldnt be. Doing things youre bad at has some serious mental, physical, and emotional benefits.
The B cell-restricted transmembrane glycoprotein CD22 is rapidly phosphorylated on tyrosine in response to cross-linking of the B cell antigen receptor, thereby generating phosphotyrosine motifs in the cytoplasmic domain which recruit intracellular effector proteins that contain Src homology 2 domains. By virtue of its interaction with these effector proteins CD22 modulates signal transduction through the B cell antigen receptor. To define further the molecular mechanism by which CD22 mediates its co-receptor function, phosphopeptide mapping experiments were conducted to determine which of the six tyrosine residues in the cytoplasmic domain are involved in recruitment of the stimulatory effector proteins phospholipase Cχ (PLCχ), phosphoinositide 3-kinase (PI3K), Grb2, and Syk. The results obtained indicate that the protein tyrosine kinase Syk interacts with multiple CD22- derived phosphopeptides in both immunoprecipitation and reverse Far Western assays. In contrast, the Grb2·Sos complex was ...
YWHAE - YWHAE (Myc-DDK-tagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1 available for purchase from OriGene - Your Gene Company.
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Traditionally we have included everything in the SDK needed to build the SDK. This implies including EGit/JGit in the Eclipse SDK. However this introduces a cyclic build dependency which always causes problems and introduces end-game risk. We need to reconsider this policy of including everything we need. Using p2 having multiple "root" features installed and updating them on an ongoing basis is no longer painful. The advantage of putting everything into a single build is perhaps outweighed by the pain of build-time dependency entanglement. Recommendation: Remove all VCM support from the Eclipse SDK and users install what they need separately. Need to discuss this at the PMC call and architecture meeting to find consensus ...
... is located in Burnett County, Wisconsin. This lake is 330 acres in size. It is approximately 29 feet deep at its deepest point. Anglers can expect to catch a variety of fish including Bluegill, Largemouth Bass, Muskellunge (Muskie), Northern Pike, Tiger Muskellunge (Tiger Muskie) and Walleye.
Myotonia is a state of hyperexcitability of skeletal-muscle fibres. Mutations in the ClC-1 Cl− channel cause recessive and dominant forms of this disease. Mutations have been described throughout the protein-coding region, including three sequence variations (A885P, R894X and P932L) in a distal C-terminal stretch of residues [CTD (C-terminal domain) region] that are not conserved between CLC proteins. We show that surface expression of these mutants is reduced in Xenopus oocytes compared with wild-type ClC-1. Functional, biochemical and NMR spectroscopy studies revealed that the CTD region encompasses a segment conserved in most voltage-dependent CLC channels that folds with a secondary structure containing a short type II poly-proline helix. We found that the myotonia-causing mutation A885P disturbs this structure by extending the poly-proline helix. We hypothesize that this structural modification results in the observed alteration of the common gate that acts on both pores of the channel. ...
FMR1 is an RNA-binding protein that is either absent or mutated in patients affected by the fragile X syndrome, the most common inherited cause of mental retardation in humans. Sequence analysis of the FMR1 protein has suggested that RNA binding is related to the presence of two K-homologous (KH) modules and an RGG box. However, no attempt has been so far made to map the RNA-binding sites along the protein sequence and to identify possible differential RNA-sequence specificity. In the present article, we describe work done to dissect FMR1 into regions with structurally and functionally distinct properties. A semirational approach was followed to identify four regions: an N-terminal stretch of 200 amino acids, the two KH regions, and a C-terminal stretch. Each region was produced as a recombinant protein, purified, and probed for its state of folding by spectroscopical techniques. Circular dichroism and NMR spectra of the N-terminus show formation of secondary structure with a strong tendency to ...
Activity:. TMP195 is a selective class IIa histone deacetylase (HDAC) inhibitor with an IC50 of 300 nM. IC50 & Target: IC50: 300 nM (HDAC), 9 nM (HDAC9), 46 nM (HDAC7), 106 nM (HDAC5) , 111 nM (HDAC4), 11700 nM (HDAC8), 47800 nM (HDAC6)[1] In Vitro: TMP195 blocks the accumulation of CCL2 protein in the supernatants of monocyte-derived macrophage differentiation cultures. TMP195 significantly increases the amount of CCL1 protein secreted by the monocytes compared to vehicle group. In the transcriptional profiling data from the PHA-stimulated PBMC experiments, CCL2 and CCL1 are respectively down- or upregulated by TMP195[1]. TMP195 occupies the acetyllysine-binding site of class IIa HDACs. TMP195 competes against binding of HDAC7 to a variety of side-chain modifications on the same peptide backbone, despite no interference with the activity of other acetyllysine reader proteins BRD4 (IC50>50 μM)[2]. In Vivo: TMP195 treatment alters the tumour microenvironment and reduces tumour burden and ...
FingerCell 3.1 SDK is based on FingerCell 3.1 technology that is specially designed for integrating biometric fingerprint recognition into hardware with low-power, low-memory microcontrollers. The fingerprint templates created with FingerCell SDK are compatible with VeriFinger SDK, MegaMatcher SDK and MegaMatcher On Card SDK biometric technologies. Also, FingerCell SDK is compatible with third-party biometric systems, as it accepts and generates fingerprint templates in ISO/IEC 19794-2 and ANSI/INCITS 378 formats. The following types of FingerCell 3.1 SDK are available: ...
KAI Pharmaceuticals (now Amgen) was developing KAI 1455, a selective epsilon protein kinase C (εPKC) activator, to prevent ischaemic injury during surgical
sdk.environment=production;sdk.charset=UTF-8;api.endpoint=http://ixfd-api.bc0a.com/;sdk.account=f00000000154372;sdk.connectTimeout=500;sdk.socketTimeout=500;sdk.crawlerConnectTimeout=750;sdk.crawlerSocketTimeout=750;whitelist.parameter.list=ixf;flat.file=true;sdk.proxyPort=0;sdk.proxyProtocol=http;crawler.useragents=google,bingbot,msnbot,slurp,duckduckbot,baiduspider,yandexbot,sogou,exabot,facebot,ia_archiver;forcedirectapi.parameter.list=ixf-api,ixf;page.alias.url=https://isg-one.com/research/isg-performex/articles;canonical.host=isg-one.com;canonical.protocol= ...
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sdk.environment=production;sdk.charset=UTF-8;api.endpoint=https://ixf2-api.bc0a.com/;sdk.account=f00000000112204;sdk.connectTimeout=2000;sdk.socketTimeout=2000;sdk.crawlerConnectTimeout=10000;sdk.crawlerSocketTimeout=10000;whitelist.parameter.list=ixf;flat.file=true;sdk.proxyPort=0;sdk.proxyProtocol=http;crawler.useragents=google,bingbot,msnbot,slurp,duckduckbot,baiduspider,yandexbot,sogou,exabot,facebot,ia_archiver;canonical.page=https://www.cedarville.edu/Offices/Human-Resources/Doctrinal-Questionnaire.aspx;page.hide.originalurl= ...
Appcelerator announces general availability for the Titanium CLI, SDK and Appcelerator Studio (LiveView) that offer compatibility with Node 0.12.
Process has been suspended. This event can be used by the debugger module to signal if the process spontaneously gets suspended (not because of an exception, breakpoint, or single step). IDA will silently switch to the suspended process mode without displaying any messages. ...
Hey everyone, I just did a swap of the analog DIM cluster from my 2012 S60 to a newer style TFT DIM from the 2014+ S60 models. Theres a good amount of information on this installation already, so Ill preface this thread with a link to the long running thread discussion. https://forums.swedespeed.com/showthread.php?197030-2014-TFT-Gauge-Cluster-DIM-Retrofit There had been talk here and there that I had found in the past on getting everything but the Power/Eco gauges to work, but I
TY - JOUR. T1 - Blue-Light- and Phosphorylation-Dependent Binding of a 14-3-3 Protein to Phototropins in Stomatal Guard Cells of Broad Bean. AU - Kinoshita, Toshinori. AU - Emi, Takashi. AU - Tominaga, Misumi. AU - Sakamoto, Koji. AU - Shigenaga, Ayako. AU - Doi, Michio. AU - Shimazaki, Ken Ichiro. PY - 2003/12/1. Y1 - 2003/12/1. N2 - Phototropins are blue-light (BL) receptor serine (Ser)/threonine kinases, and contain two light, oxygen, and voltage (LOV) domains, and are members of the PAS domain superfamily. They mediate phototropism, chloroplast movement, leaf expansion, and stomatal opening of higher plants in response to BL. In stomatal guard cells, genetic analysis has revealed that phototropins mediate activation of the plasma membrane H+-ATPase by phosphorylation and drive stomatal opening. However, biochemical evidence for the involvement of phototropins in the BL response of stomata is lacking. Using guard cell protoplasts, we showed that broad bean (Vicia faba) phototropins (Vfphots) ...
Gentaur molecular products has all kinds of products like :search , Prospecbio \ Mouse Anti Human Tyr-3_trp-5 Monooxygenase Activation Protein Epsilon YWHAE \ ant-379 for more molecular products just contact us
Download PlugPDF SDK - PlugPDF SDK is a simple, cost-effective and streamlined PDF reader SDK. Designed to provide optimum support on both Android and iOS platforms, you can create a high performance mobile PDF reader application quickly and effectively with PlugPDF SDK.
The receptor for the wilt-inducing phytotoxin fusicoccin was purified to homogeneity from plasma membranes of Commelina communis as a complex with the radioligand [3H]9-nor-8-hydroxyfusicoccin. The preparation consisted of two polypeptides with apparent molecular masses of 30.5 kDa and 31.5 kDa an …
The Apache Kafka NodeJS SDK by SOHU-Co allows developers to integrate the Apache Kafka APIs into their NodeJS applications. This SDK is compatible with Apache Kafka 0.9+.
CCM (Citrix Common Connection Manager) SDK provides APIs which can be used to get the ICA session information. This can be programmatically used in custom application to modify the appearance for the end user.
... (aka p+) is a component based C++ framework that is optimized for GL/GLES based mobile cross-platform game development.
... (aka p+) is a component based C++ framework that is optimized for GL/GLES based mobile cross-platform game development.
The Portable SDK for UPnP Devices libupnp library contains multiple buffer overflow vulnerabilities. Devices that use libupnp may also accept UPnP queries over the WAN interface, therefore exposing the vulnerabilities to the internet.
MSF兔多克隆抗体(ab114099)可与人样本反应并经IP, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
In this study,YWHAE expression was silenced by RNA interference in colon cancer cell lines, and investigate the correlation between YWHAE expression and colon cancer proliferation,and differential expressed genes were analized by gene assay and qPCR technologe.The proliferation and colony formation ability of colon cancer cells were significantly reduced after YWHAE knockdown. More 1200 known genes were found to be involved in the YWHAE functions related tumorigenesis by genechip analysis, these genes related to cell proliferation/apoptosis and cell cycle process. qRT-PCR results showed an expression pattern consistent with that of the genechip analysis. Lentivirus-mediated RNA interference was used to knockdown YWHAE expression in colon cancer cell lines. And cell proliferation assays . GeneChip analysis were carried out for mRNA expression profiling, followed, the expressed levels of mRNA and protein were measured by Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot ,
Pseudomonas are a common cause of hospital-acquired infections that may be lethal. ADP-ribosyltransferase activities of Pseudomonas exotoxin-S and -T depend on 14-3-3 proteins inside the host cell. By binding in the 14-3-3 phosphopeptide binding groove, an amphipathic C-terminal helix of ExoS and ExoT has been thought to be crucial for their activation. However, crystal structures of the 14-3-3 beta: ExoS and -ExoT complexes presented here reveal an extensive hydrophobic interface that is sufficient for complex formation and toxin activation. We show that C-terminally truncated ExoS ADP-ribosyltransferase domain lacking the amphipathic binding motif is active when co-expressed with 14-3-3. Moreover, swapping the amphipathic C-terminus with a fragment from Vibrio Vis toxin creates a 14-3-3 independent toxin that ADP-ribosylates known ExoS targets. Finally, we show that 14-3-3 stabilizes ExoS against thermal aggregation. Together, this indicates that 14-3-3 proteins activate exotoxin ...
Visit Healthgrades for information on Dr. Lilaine Leonardo, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
We love to SDK. Its the 5th stroke. It takes skill, strength, flexibility, conditioning & mental toughness. For many its the 2nd fastest stroke, but its not a legal stroke. We wish it was legal. We wish there werent 15 m restrictions in races. We count our kicks because kicks count. We train to SDK faster. Some call SDKs underwaters or dolphins. What are you doing to improve your SDK? How many do you take in each race?
"Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins". Proteins. 65 (3): 643-55. doi: ... Notably, the protein contains three EF hand motifs that function as binding sites for Ca2+ ions. The protein is a member of the ... In the animals, the protein occurs together with the Green fluorescent protein to produce green light by resonant energy ... Since then, the protein has been widely used as reported in many model biological systems, including zebrafish, rats, mice, and ...
Protein Science. 14 (3): 812-822. doi:10.1110/ps.04951405. ISSN 0961-8368. PMC 2279270 . PMID 15722453. Hulver MW, Berggren JR ... "Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B ... "Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B ... 3 (4): 271-80. doi:10.2174/1568008033340117. PMID 14683458. Shiwaku K, Hashimoto M, Kitajima K, Nogi A, Anuurad E, Enkhmaa B, ...
Hydrogen bonds are (red) between protein, water (blue balls) and the cofactor PLP (purple). Figure 4 shows the alpha helices ( ... Koyata H, Hiraga K (1991). "The glycine cleavage system: Structure of a cDNA encoding human H-protein, and partial ... Protein Science. 14 (3): 791-8. doi:10.1110/ps.041179105. PMC 2279282 . PMID 15689518. "KEGG ENZYME Database Entry". Kyoto ... its activity is augmented by high-protein diets and starvation. During periods of low carbohydrates, serine is converted into ...
Users can search the database for a particular motion by either protein name or Protein Data Bank ID number. Typically, however ... ISBN 0-470-18105-2. "Chapter 26 on Protein Motions". The Physics of Proteins: An Introduction to Biological Physics and ... users will enter the database via the Protein Data Bank, which often provides a hyperlink to the molmovdb entry for proteins ... Database of protein conformational diversity Gu, Jenny; Bourne, Philip E. (March 2009). Structural Bioinformatics (2nd ed.). ...
protein binding. • MHC class II protein complex binding. • protein heterodimerization activity. • enzyme binding. • poly(A) RNA ... identical protein binding. • macromolecular complex binding. • cadherin binding. • scaffold protein binding. Cellular component ... protein localization to nucleus. • cellular response to heat. • positive regulation of protein export from nucleus. • ... protein domain specific binding. • protein complex binding. • histone deacetylase binding. • potassium channel regulator ...
The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has ... Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53-63. doi ... "Protein kinase CK2 regulates CDC25B phosphatase activity". Oncogene. England. 22 (2): 220-32. doi:10.1038/sj.onc.1206107. ISSN ... 14 (20): 2485-95. doi:10.1038/sj.onc.1201063. PMID 9188863. Booher RN, Holman PS, Fattaey A (1997). "Human Myt1 is a cell cycle ...
Forkhead box O6 is a protein that in humans is encoded by the FOXO6 gene. GRCm38: Ensembl release 89: ENSMUSG00000052135 - ... 5 (3): 233-40. doi:10.1111/1753-0407.12027. PMC 3657578 . PMID 23324123. Qinyu L, Long C, Zhen-dong D, Min-min S, Wei-ze W, Wei ... Tzivion G, Dobson M, Ramakrishnan G (November 2011). "FoxO transcription factors; Regulation by AKT and 14-3-3 proteins". ...
M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A is a protein that in humans is encoded by the ... In contrast, some invertebrates harbour 2 (e.g., the Drosophila proteins String and Twine) or four (e.g., C. elegans Cdc-25.1 ... Dual-specificity protein phosphatases remove phosphate groups from phosphorylated tyrosine and serine / threonine residues. ... Coqueret O, Bérubé G, Nepveu A (1998). "The mammalian Cut homeodomain protein functions as a cell-cycle-dependent ...
Raf kinases A-Raf kinase B-Raf kinase KSR1 protein KSR2 protein GRCm38: Ensembl release 89: ENSMUSG00000000441 - Ensembl, May ... Yuryev A, Wennogle LP (February 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid ... a novel jun N-terminal protein kinase (JNK)-binding protein that functions as a Scaffold factor in the JNK signaling pathway". ... and the enzymes dedicated for removal of these phosphates are the protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A ...
Mitogen-activated protein kinase kinase kinase 2 is an enzyme that in humans is encoded by the MAP3K2 gene. The protein encoded ... Cheng J, Zhang D, Kim K, Zhao Y, Zhao Y, Su B (Jul 2005). "Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and ... This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a ... Yan M, Dai T, Deak JC, Kyriakis JM, Zon LI, Woodgett JR, Templeton DJ (1995). "Activation of stress-activated protein kinase by ...
"A helix-loop-helix protein related to the immunoglobulin E box-binding proteins". Molecular and Cellular Biology. 10 (8): 4384- ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ... Transcription factor EB is a protein that in humans is encoded by the TFEB gene.[5][6] ... 21] TFEB nuclear export is mediated by CRM1 and is dependent on phosphorylation.[22][23] TFEB is also a target of the protein ...
One such protein is rad18 that is required for G2 arrest even when chk1 is phosphorylated and active. This protein is also ... The cell cycle is driven by proteins called cyclin dependent kinases that associate with cyclin regulatory proteins at ... Proteins that localize to sites of DNA damage in the G2 phase initiate a signaling cascade that regulates wee1 and cdc25 ... Proteins that function in the G2-M checkpoint were originally identified in yeast screens that looked for mutants which show ...
... protein, human at the US National Library of Medicine Medical Subject Headings (MeSH). ... or K2P18.1 is a protein that in humans is encoded by the KCNK18 gene. K2P18.1 is a potassium channel containing two pore- ... Czirják G, Vuity D, Enyedi P (2008). "Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation". J. Biol ...
The protein can be a toxin, an apoptotic factor, or a viral protein. Toxins such as diphtheria toxin interfere with cellular ... "Regulation of telomerase activity and anti-apoptotic function by protein-protein interaction and phosphorylation". FEBS Lett. ... Viral proteins like viral thymidine kinase can be used for specific targeting of a drug. By introducing a prodrug only ... Song H, Li Y, Chen G, Xing Z, Zhao J, Yokoyama KK, Li T, Zhao M (April 2004). "Human MCRS2, a cell-cycle-dependent protein, ...
... camp-responsive element-binding protein (CREB) and CREB-binding protein (CBP). It has been proposed that recruitment of ... Mutant atrophin-1 proteins have been found in neuronal intranuclear inclusions (NII) and diffusely accumulated in the neuronal ... Atrophin-1 may be a dispensable or redundant protein as mice bred with a null allele for atrophin-1 produce viable and fertile ... It contains a putative nuclear localization signal in the N-terminus of the protein and a putative nuclear export signal in the ...
This protein family is known for regulating signal transduction pathways primarily through binding phosphoserine proteins, ... is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. The protein encoded by this gene is a member of the ... though it can also bind phosphothreonine proteins and unphosphorylated proteins. By extension, 14-3-3 proteins are involved in ... "New insights into potential functions for the protein 4.1 superfamily of proteins in kidney epithelium". Frontiers in ...
T-cell leukemia homeobox protein 2 is a protein that in humans is encoded by the TLX2 gene. TLX2 has been shown to interact ... 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMC 528928 . PMID 15489334. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ... Tang SJ, Suen TC, McInnes RR, Buchwald M (1998). "Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins". J. ... "Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins". J. Biol. Chem. UNITED STATES. 273 (39): 25356-63. doi: ...
ER retention refers to proteins that are retained in the endoplasmic reticulum, or ER, after folding; these are known as ER ... The classical ER retention signal is the C-terminal KDEL sequence for lumen bound proteins and KKXX for transmembrane ... These signals allow for retrieval from the Golgi apparatus by ER retention receptors, effectively maintaining the protein in ... resident proteins. Their localization to the ER often depends on certain sequences of amino acids located at the N-terminus or ...
The cause was determined to be a histidine-to-tyrosine (amino acid) substitution at position 274 in its neuraminidase protein. ... A single hemagglutinin-neuraminidase protein can combine neuraminidase and hemagglutinin functions, such as in mumps virus and ... Neuraminidase also cleaves sialic acid residues from viral proteins, preventing aggregation of viruses. Neuraminidase has been ... Proteins: Structure, Function, and Genetics. 14 (3): 327-32. doi:10.1002/prot.340140302. PMID 1438172. Huang IC, Li W, Sui J, ...
Anson ML, Protein Denaturation and the Properties of Protein Groups, Advances in Protein Chemistry, 2, 361-386 (1945) ... Protein Sci. 4(10), 2138-2148 (1995) Robertson, A.D., Murphy, K.P. Protein structure and the energetics of protein stability, ( ... 1992) "Physical Basis for the Stability of the Folded Conformations of Proteins", in Protein Folding, TE Creighton, ed., W. H. ... "The use of high-pressure nuclear magnetic resonance to study protein folding". In Bai, Yawen; Nussinov, Ruth. Protein folding ...
... is a mammalian gene encoding the protein CBL which is an E3 ubiquitin-protein ligase involved in cell signalling and protein ... "The Cbl proteins are binding partners for the Cool/Pix family of p21-activated kinase-binding proteins". FEBS Lett. 550 (1-3): ... "The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src ... "The protein product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the ...
... the protein import tunnel Toc75, plus the proteins Toc64 and Toc12. The first three proteins form a core complex that consists ... A protein kinase drifting around on the outer chloroplast membrane can use ATP to add a phosphate group to the Toc34 protein, ... This loss of GTP makes the Toc34 protein release the chloroplast preprotein, handing it off to the next TOC protein. Toc34 then ... Hundreds of different PPR proteins from the nuclear genome are involved in the RNA editing process. These proteins consist of ...
Mutant p53 proteins often fail to induce MDM2, causing p53 to accumulate at very high levels. Moreover, the mutant p53 protein ... In addition to the full-length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to ... One such example, human papillomavirus (HPV), encodes a protein, E6, which binds to the p53 protein and inactivates it. This ... The full length p53 isoform proteins can be subdivided into different protein domains. Starting from the N-terminus, there are ...
... allowing for Rab protein to change from its GDP to GTP bound state. Inhibition of the GTPase-activating domain leaves proteins ... Like all proteins, the unique amino acid arrangement in the primary sequence of GLUT4 are what allow it to transport glucose ... Two proteins with capacity to bind Ubc9 and conjugated to SUMO1". The Journal of Biological Chemistry. 277 (22): 19783-91. doi: ... GLUT4 Protein at the US National Library of Medicine Medical Subject Headings (MeSH) USCD-Nature molecule pages: The signaling ...
The alpha-2B adrenergic receptor (α2B adrenoceptor), is a G-protein coupled receptor. It is a subtype of the adrenergic ... This gene encodes the α2B subtype, which was observed to associate with eIF-2B, a guanine nucleotide exchange protein that ... Comparative analysis of the primary protein sequence of ADRA2B across placentals also showed the high conservation of residues ... "Regulation of adenylyl cyclase in polarized renal epithelial cells by G protein-coupled receptors". The American Journal of ...
... is a subjective symptom of fatigue that is experienced by nearly all cancer patients. Among patients receiving cancer treatment other than surgery, it is essentially universal. Fatigue is a normal and expected side effect of most forms of chemotherapy, radiation therapy, and biotherapy. On average, cancer-related fatigue is "more severe, more distressing, and less likely to be relieved by rest" than fatigue experienced by healthy people. It can range from mild to severe, and may be either temporary or a long-term effect. Fatigue may be a symptom of the cancer, or it may be the result of treatments for the cancer. The National Comprehensive Cancer Network defines cancer-related fatigue as "a distressing persistent, subjective sense of physical, emotional and/or cognitive tiredness or exhaustion related to cancer or cancer treatment that is not proportional to recent activity and interferes with usual functioning". Cancer-related fatigue is a chronic fatigue (persistent ...
... is intended to reduce fatigue related fatalities and incidents. Several companies are working on a technology for use in industries such as mining, road- and rail haulage and aviation. The technology may soon find wider applications in industries such as health care and education. In an operational environment scenario where operating systems are dependent on human performance, fatigue can be defined as an inclination to degrade performance. Thus, fatigue is an indicator of baseline risk for the occurrence of errors and accidents. Globally mining operations are at risk of fatigued workers. Sleepiness and fatigue increase human error and contribute to accidents which can be fatal. Factors compounding fatigue levels in mine workers include; disruptions in circadian rhythms due to shift work, exposure to noise, vibration and chemicals, monotonous and repetitive nature of tasks and night shift driving. Studies recognise a connotation between lifestyle and fatigue. Mine ...
... , or central fatigue, is a form of fatigue that is associated with changes in the synaptic concentration of neurotransmitters within the central nervous system (CNS; including the brain and spinal cord) which affects exercise performance and muscle function and cannot be explained by peripheral factors that affect muscle function. In healthy individuals, central fatigue can occur from prolonged exercise and is associated with neurochemical changes in the brain, primarily involving serotonin (5-HT), noradrenaline, and dopamine. Central fatigue plays an important role in endurance sports and also highlights the importance of proper nutrition in endurance athletes. Existing experimental methods have provided enough evidence to suggest that variations in synaptic serotonin, noradrenaline, and dopamine are significant drivers of central nervous system fatigue. An increased synaptic dopamine concentration in the CNS is strongly ergogenic (promotes exercise performance). ...
ME happens when a low-melting-point metal is placed in contact with a metal with higher melting point, so the latter is embrittled. It is often manifested by static fatigue. For example, as shown in Figure 1,[2] in a test of the static fatigue of a 2024 aluminum coated with mercury, the alloy is subject to a stress level less than the value causing plastic flow, and the time it takes to fracture is measured. Usually a stress called static fatigue limit is present, representing the boundary below which the material does not fracture, no matter how long the test duration is. In this scenario, the static fatigue often depends on the presence of initial flaws. Also, if the material is "flaw-free", its static fatigue limit serves as a design parameter in a hostile environment. ...
Modern technology seeks to minimize or prevent listener fatigue entirely. Blockage of the ear canal, common in [headphones], is thought to be a main contributing factor in listener fatigue. When cut off from outside sound with the earphone, an oscillating pressure chamber is created in the eardrum. This effectively provides a boost in sound pressure levels. When this boost occurs, an acoustic reflex mechanism triggers and acts as a defense against these sounds. This mechanism seeks to reduce the sound energy in the ear by dampening its transfer from eardrum to cochlea. It has been seen that this process can reduce sound waves by up to 50 decibels. Although this mechanism can decrease the sound energy, it does not negate the oscillatory pressure. Due to this defense mechanism, sounds do not seem as loud as they are, and ironically, listeners will want to increase the volume. As a result, the reflex mechanism is activated again, and the cycle continues on. This ultimately leads to fatigue. ...
The second phase of Ranger School is conducted at the remote Camp Merrill near Dahlonega, Georgia by the 5th Ranger Training Battalion. Here, "students receive instruction on military mountaineering tasks, mobility training, as well as techniques for employing a platoon for continuous combat patrol operations in a mountainous environment".[20] Adding to the physical hardships endured in the Benning phase, in this phase "the stamina and commitment of the Ranger student is stressed to the maximum. At any time, he or she may be selected to lead tired, hungry, physically expended students to accomplish yet another combat patrol mission".[20] The Ranger student continues learning how to sustain themselves and their subordinates in the mountains. The rugged terrain, severe weather, hunger, mental and physical fatigue, and the psychological stress the student encounters allow them to measure their capabilities and limitations and those of their fellow soldiers. In addition to combat operations, the ...
Nerves are responsible for controlling the contraction of muscles, determining the number, sequence and force of muscular contraction. Most movements require a force far below what a muscle could potentially generate, and barring pathological nervous fatigue, is seldom an issue[vague][clarification needed]. But in extremely powerful contractions that are close to the upper limit of a muscle's ability to generate force, nervous fatigue (enervation), in which the nerve signal weakens, can be a limiting factor in untrained individuals. In novice strength trainers, the muscle's ability to generate force is most strongly limited by nerve's ability to sustain a high-frequency signal. After a period of maximum contraction, the nerve's signal reduces in frequency and the force generated by the contraction diminishes. There is no sensation of pain or discomfort, the muscle appears to simply 'stop listening' and gradually cease to move, often going backwards. As there is insufficient stress on the muscles ...
In June 2017, the U.S. Centers for Disease Control and Prevention stated that speaking with a therapist may help.[88] Previously, a 2014 National Institutes of Health report concluded that while counseling and behavior therapies could produce benefits for some people, they may not yield improvement in quality of life, and because of this limitation such therapies should not be considered as a primary treatment, but rather should be used only as one component of a broader approach.[89] This same report stated that although counseling approaches have shown benefit in some measures of fatigue, function and overall improvement, these approaches have been inadequately studied in subgroups of the wider CFS patient population. Further concern was expressed that reporting of negative effects experienced by patients receiving counseling and behavior therapies had been poor.[86] A report by the Institute of Medicine published in 2015 states that it is unclear whether CBT helps to improve cognitive ...
Metastaasiks ehk siirdeks ehk teiseseks haiguskoldeks (ladina metastasis) on mitmetel loomadel esineda võiv metastaatiline haiguslik seisund. Metastaasidele on iseloomulik pahaloomuliste kasvajarakkude, mikroobide levik algkoldest, peamiselt vereringe ja lümfisõlmede ning lümfisüsteemi vahendusel, organismi teistesse elunditesse. Peamiselt peetakse metastaaside puhul silmas siiski pahaloomulise kasvaja algkoldest väljaspool paikneva teisese koldega seonduvat. Kõik pahaloomulised kasvajad võivad metastaseeruda. Kui kasvaja rakk ja/või rakud erinevate tegurite kaasabil metastaseeruvad ja teises elundis paljunevad ning moodustavad seal veresoonkonnavälise kasvava massi, siis nimetatakse uut kasvajakollet rakkude sarnasuse tõttu teiseseks kasvajakoldeks. Metastaaside tavalised paikmed on luud, peaaju, diafragma, süda, maks, kops, lümfisõlmed, keskseinand, ajukelmed, kõhukelme, pleura, retroperitoneum, seljaaju.[1]. Üksikute kasvajarakkude levikut ehk mikrometastaase pole võimalik ...
Hodgkini lümfoomi levinuimad sümptomid on kaela, kaenlaaluse või vaagna piirkonnas paikneva ühe või mitme lümfisõlme, harilikult valutu, suurenemine. Lümfoomi korral suurenevad lümfisõlmed aeglaselt ja võib kuluda kuid või aastaid, enne kui neid märgatakse. Mõnikord võivad lümfisõlmed suureneda väga kiiresti.[11] Rohkem kui 80%-l patsientidest on leitud lümfadenopaatia ülalpool diafragmat.[2] Kolmandikul (25%) patsientidest esineb Hodgkini lümfoomi korral ka üldsümptomeid (nn B-sümptomid), näiteks öine higistamine, kõrge kehatemperatuur, mis tõuseb ja alaneb spontaanselt, märgatav kaalukaotus lühikese aja jooksul, sügelemine, mis võib pärast alkoholi seespidist tarbimist intensiivistuda, köha või hingeldus, kõhuvalu või oksendamine pärast alkoholi joomist.[11] ...
Interleukiin-10 ehk IL-10 on paljude selgroogsete loomade mitmete lümfisüsteemi rakkude poolt teatud sündmuste korral vabastatavad endogeensed lühiajalised valgulised signaalmolekulid, mis vahendavad tõenäoliselt põletikuvastaseid jaimmunosupressiivseid toimeid (immuunvastuse pidurdamine) ning mis liigitatakse põletikuvastaste tsütokiinide hulka. IL-10-t toodavad ja vabastavad T- (CD4 T-rakud, CD8 T-rakud jt), B-rakud, monotsüüdid, dendriitrakud ja makrofaagid, aga ka kesknärvisüsteemi liigitatud gliiarakud jpt. Eri tüüpi tsütokiinid kasutavad nn segasignaale, nii näiteks inhibeerib IL-4 ja IL-10 eritamine Th1- tüüpi immuunvastuste supressiooni, vähendamaks makrofaagidelt tuleva IL-12 tootmist. Põletikutoime arvatakse seisnevat põletikutsütokiinide, nagu IFN-γ, IL-2, IL-3, TNFα ja GM-CSF, sünteesi inhibeerimises. Interleukiin-10 on 2. rühma (IL10- perekonna), mis koosneb interleukiin-10, IL-19, IL-20, IL-22, IL-24 (Mda-7) ja IL-26, interferoonidest (IFN-alfa, -beeta, ...
TIL-rakud ehk tuumorikoe lümfotsüüdid ehk kasvajasse infiltreeruvad lümfotsüüdid (inglise tumor-infiltrating lymphocyte) on osade selgroogsete uudismoodustistes funktsioneeriv rakutüüp.[1] Osadesse kasvajatesse infiltreeruvad TIL-rakud loetakse lümfisüsteemi (lümfoid(-immuun)süsteemi) rakupopulatsioonide - leukotsüütide hulka ja neile omistatakse osa organismi kasvajavastases immuunvastuses. Arvatakse, et TIL-rakud on püsivad kasvajarakkude kaaslased ja nende osa seostatakse enim kasvajarakkude hävitamisega. ...
Roomavat madarat kasutatakse ravimtaimena. Ürt (Galii aparinis herva) on taime maapealne osa. Ürti kogutakse taime õitsemise ajal ja kuivatatakse. Droog sisaldab iridoide, flavonoide, antratseene, kumariine, tanniine, orgaanilisi happeid, mineraalaineid (vask, tsink, raud, mangaan, boor, titaan, strontsium, vanaadium, tallium, nikkel, molübdeen), rasvõli jpm.[4] Droogil on uriinieritust suurendav toime. Kasutatakse düsuuria, kuseteede, lümfisõlme-, mao- ja peensoolepõletiku, haavandtõve ja palaviku puhul. Suurendab sapinõristust.[4] Rahvameditsiinis peetakse teda põletiku-, mikroobi-, oksüdeerumis- ja vähivastaseks.[6] ...
Proportion of tau protein to phosphorylated tau protein CSF levels in differential diagnosis of dementia. Nervenarzt 2008; 79(8 ... Protein 14-3-3 Detection in Cerebrospinal Fluid - Clinico-Pathological Correlation Introduction:. The 14-3-3 - protein group is ... Protein 14-3-3 detection was performed by a standardized method using western blot with chemoluminiscent detection of ß-subunit ... Protein 14-3-3 byl poprvé popsán Moorem a Pertezem v roce 1967 [1] jako významná kyselá frakce mozkových proteinů. Skupinu ...
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... 14-3-3 protein homolog. Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast). Loading... ... 14-3-3-like protein. Oenothera elata subsp. hookeri (Hookers evening primrose). Loading... ...
2006 Jun;16(3):183-92. Epub 2006 Apr 1. Research Support, Non-U.S. Govt; Review ... M-transition by binding to regulatory proteins and modulating their function. In most cases, the association with 14-3-3 ... proteins requires a specific phosphorylation of the protein ligand and mediates cell cycle arrest. 14-3-3 binding may lead to ... Effects of 14-3-3 proteins on cell cycle progression and the regulation of 14-3-3 activity during the cell cycle are reviewed ...
The 14-3-3 proteins are binding proteins that have been shown to interact with a wide array of enzymes involved in primary ... In this review, we examine the factors that may regulate the binding of 14-3-3s to their target proteins, and discuss their ... In most cases, the significance of binding of the 14-3-3 protein is not known. However, most of the interactions are ... Metabolic enzymes as targets for 14-3-3 proteins.. Huber SC1, MacKintosh C, Kaiser WM. ...
... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... of PC12 proteins and identified the TH protein as a major constituent of the proteins that are co-immunoprecipitated with 14-3- ... 5 cytoskelton proteins, 18 nuclear proteins including transcription factors, 10 metabolic enzymes, and others. We previously ...
... is part of an EU Framework IV Biotech-programme entitled Central Role in Adaptation of Fourteen Three Three proteins, and is ... 6, 341-347) by serving as docking proteins for receptors, kinases and phosphatases. In plants some 14-3-3 isoforms make up the ... translocation within the cell and docking to the respective targets proteins) will be studied by means of Green Fuorescent ... Candidates should have demonstrated experience and publications in biochemistry/protein chemistry and molecular biology. ...
Reversible protein phosphorylation events are known to play a crucial role in the regulation of stomatal movements. However, in ... Among the phosphopeptide-binding proteins, the 14-3-3 proteins are the best characterized in plants. The 14-3-3s are found as ... Among the phosphopeptide-binding proteins, the 14-3-3 proteins are the best characterized in plants. The 14-3-3s are found as ... However, in some cases, phosphorylation alone is not sufficient to achieve complete protein regulation, but is necessary to ...
The Bmh2 protein is , 70% identical to the mammalian epsilon-isoform and , 90% identical to a previously reported yeast 14-3-3 ... Now we report that one of the suppressor loci, BMH2/SCD3, encodes a protein of the 14-3-3 family. The 14-3-3 proteins are ... encoding a cAMP-dependent protein kinase, restores Bmh- yeast to viability. High-copy TPK1 did not rescue Chc- yeast. These ... protein encoded by BMH1. Single deletions of BMH1 or BMH2 have no discernable phenotypes, but deletion of both BMH1 and BMH2 is ...
We suggest that the target protein is attracted first by general protein-protein interaction motifs (the desolvation patches) ... Structural basis for protein-protein interactions in the 14-3-3 protein family. Xiaowen Yang, Wen Hwa Lee, Frank Sobott, ... Approximately 2 μl of protein solution (10-30 μM) was loaded per capillary. Protein samples were buffer exchanged into 20 mM ... 4, where red patches represent likely protein-protein interaction interfaces. No low-desolvation patches were observed for any ...
14-3-3 zeta/delta Proteins available through Novus Biologicals. Browse our 14-3-3 zeta/delta Protein catalog backed by our ... MGC111427 protein, MGC138156 protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide ... Protein Array, SDS-Page, Western Blot. Each 14-3-3 zeta/delta Peptide and 14-3-3 zeta/delta Protein is fully covered by our ... Our 14-3-3 zeta/delta Peptides and 14-3-3 zeta/delta Proteins can be used in a variety of model species: Human. Use the list ...
The p53 tumour-suppressor protein is a sequence-specific DNA-binding transcription factor that induces cell cycle arrest or ... Both the levels of p53 protein and its affinity for specific DNA sequences increase in response to genotoxic stress. In vitro, ... The p53 tumour-suppressor protein is a sequence-specific DNA-binding transcription factor that induces cell cycle arrest or ... ATM-dependent activation of p53 involves dephosphorylation and association with 14-3-3 proteins Nat Genet. 1998 Jun;19(2):175-8 ...
A Number of Functions for a Numbered Protein ... A Number of Functions for a Numbered Protein ... A Number of Functions for a Numbered Protein ... A Number of Functions for a Numbered Protein ...
References for Abcams Recombinant Human 14-3-3 eta protein (ab116442). Please let us know if you have used this product in ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
Ab40510 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam provides free ... Protein kinase C inhibitor protein 1. *Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex Assays. By ... Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... This protein in other organisms (by gene name): Q2QBT8 - Giardia intestinalis 2 * A8BJ73 - Giardia intestinalis no matching PDB ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... The Protein Feature View requires a browser that supports SVG (Scalable Vector Graphics). Mouse over tracks and labels for more ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Protein Feature View of PDB entries mapped to a UniProtKB sequence * Number of PDB entries for Q6P3U9: no matching PDB entries ... This protein in other organisms (by gene name): Q6P3U9 - Homo sapiens 0 * Q32P43 - Homo sapiens no matching PDB entries ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ...
Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). ... Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... "Structural basis for protein-protein interactions in the 14-3-3 protein family.". Yang X., Lee W.H., Sobott F., Papagrigoriou E ...
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... "Structural basis for protein-protein interactions in the 14-3-3 protein family.". Yang X., Lee W.H., Sobott F., Papagrigoriou E ... PROSITE; a protein domain and family database. More...PROSITEi. View protein in PROSITE. PS00796 1433_1, 1 hit. PS00797 1433_ ...
Members of a family of highly conserved proteins, termed 14-3-3 proteins, were found by several experimental approaches to ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Optimal complex formation required the amino-terminal regulatory domain of Raf-1. The association of 14-3-3 proteins and Raf-1 ...
apoptosis, ASKI, Bad, difopein, PKB, protein kinase B, ASKI, apoptosis signal-regulating kinase I, EYFP, enhanced yellow ... domain in the regulation of apoptosis and Bcl-2-related proteins Biochem Soc Trans (February, 2000) ... Survival-promoting functions of 14-3-3 proteins S. C. Masters; S. C. Masters ... Thus, 14-3-3 may be part of the cellular machinery that maintains cell survival, and targeting 14-3-3-ligand interactions may ...
The proteins were N-terminally blocked. Internal amino acid sequences were obtained for both polypeptides of the fusicoccin- ... which comprises structurally conserved regulatory proteins of widespread occurrence and a wide range of functions. 14-3-3 ... partitioned into the phospholipid-rich fraction which contains the most hydrophobic proteins. The results discussed herein ... The fusicoccin receptor of plants is a member of the 14-3-3 superfamily of eukaryotic regulatory proteins FEBS Lett. 1994 Sep ...
These proteins contain a number of known common modification domains, including regions for divalent cation interaction, ... More than 200 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and ... To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain.[ ... They are believed to interact with the protein Activation-Induced (Cytidine) Deaminase in mediating class switch recombination ...
... about 14-3-3 PROTEINS. Search and download thousands of Swedish university dissertations. Full text. Free. ... Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein-protein interaction; GUS; Arabidopsis; 14-3-3; H -ATPase; expression; ... To date, 104 proteins have been shown to interact with 14-3-3s of which 85 are found in mammals and yeast, and 21 are found in ... Abstract : The P-type plasma membrane H+-ATPases form a group of proteins only found in plants and fungi. The pumping of ...
... proteopedia.org/wiki/index.php/14-3-3_protein_3D_structures" ... 14-3-3 protein 3D structures. From Proteopedia. Jump to: ... 3D structures of 14-3-3 protein. Updated on 25-May-2020 ...
Role of acidic chromatin proteins in high-affinity binding Biochem J (May,1976) ... Multiple roles for 14-3-3 proteins in nucleo-cytoplasmic transport A. Brunet; A. Brunet ... A. Brunet, F. Kanai, J. Stehn, M. E. Greenberg, M. B. Yaffe; Multiple roles for 14-3-3 proteins in nucleo-cytoplasmic transport ... Biochem Soc Trans 1 June 2002; 30 (3): A64. doi: https://doi.org/10.1042/bst030a064a ...
  • Skupinu proteinu 14-3-3 tvoří sedm izotypových signálních molekul, jejichž přesná funkce však doposud není známa zcela jednoznačně. (csnn.eu)
  • Nález ß-podjednotky proteinu 14-3-3 v cerebrospinálním likvoru je jedním z hlavních paraklinických diagnostických markerů sporadické formy Creutzfeldtovy-Jakobovy nemoci (sCJN). (csnn.eu)
  • Průkaz proteinu 14-3-3 byl prováděn standardizovanou metodou western blot s chemiluminiscenční detekcí ß-podjednotky ve vzorku likvoru pacientů s podezřením na CJN. (csnn.eu)
  • Z celkového počtu 280 likvorových vzorků bylo 57 pozitivních v průkazu proteinu 14-3-3 a 20 nálezů bylo pozitivních hraničně/slabě. (csnn.eu)
  • Ve 25 případech pozitivity proteinu 14-3-3 v likvoru a u šesti hraničních nálezů autopsie potvrdila prionové onemocnění typu CJN. (csnn.eu)
  • V 18 případech později hodnocených jako prokázaná CJN bylo vyšetření proteinu 14-3-3 negativní. (csnn.eu)
  • Naše výsledky odpovídají literárním údajům o rozporuplnosti stanovování ß-podjednotky proteinu 14-3-3 v likvoru. (csnn.eu)
  • V porovnání s recentními publikacemi je senzitivita a specificita průkazu proteinu 14-3-3 v likvoru pro diagnózu CJN v našem souboru významně nižší, což může být ovlivněno menším počtem vyšetřených pacientů. (csnn.eu)
  • Průkaz proteinu 14-3-3 v likvoru je dle doporučení WHO jednou z podmínek pro klinickou diagnózu CJN. (csnn.eu)
  • Because of its relatively low specificity it is important to consider the relevance of 14-3-3 positive test in each particular clinical case. (csnn.eu)
  • abstract = "The filamentous fungus Trichoderma reesei is a biotechnologically important organism having an excellent capability to secrete hydrolytic enzymes e.g. cellulases.Allthough the protein production technology is well established, the protein secretion apparatus of T. reesei is still insufficiently known. (vtt.fi)
  • TCR engagement activates the protein tyrosine kinases Lck, Fyn, and ζ chain-associated protein of 70 kD that initiate the signaling cascade and contribute to the assembly of a "signalosome," a multiprotein complex including various enzymes, their substrates, and scaffold/adaptor proteins ( 4 ). (rupress.org)
  • The name for the protein family is tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein family (YWHA). (biomedcentral.com)
  • Tyrosine hydroxylase or tyrosine 3-monooxygenase is the enzyme responsible for catalyzing the conversion of the amino acid L -tyrosine to L -3,4-dihydroxyphenylalanine ( L -DOPA). (wikipedia.org)
  • Tyrosine hydroxylase catalyzes the reaction in which L -tyrosine is hydroxylated in the meta position to obtain L -3,4-dihydroxyphenylalanine ( L -DOPA). (wikipedia.org)
  • Tryptophan is a poor substrate for tyrosine hydroxylase, however it can hydroxylate L -phenylalanine to form L -tyrosine and small amounts of 3-hydroxyphenylalanine. (wikipedia.org)
  • 14-3-3 proteins play a key role in coordinating multiple signalling pathways (see Aitken 1996, Trends Cell Biol. (bio.net)
  • These genetic interactions suggest that budding-yeast 14-3-3 proteins are multifunctional and may play a role in both vesicular transport and Ras signaling pathways. (pnas.org)
  • Calorie restriction (CR) and conserved nutrient-sensing target of rapamycin (TOR) and protein kinase A (PKA) pathways have also been suggested to extend life span by increasing stress response, which protects cells from age-dependent accumulation of oxidative damages. (genetics.org)
  • 4. Nagy, G., Oostenbrink, C., and Hritz, J. Exploring the binding pathways of the 14-3-3ζ protein: Structural and free-energy profiles revealed by Hamiltonian replica exchange molecular dynamics with distancefield distance restraints. (findaphd.com)
  • G protein-coupled receptor signaling pathways mediate the transmission of signals from the extracellular environment to the generation of cellular responses, a process that is critically important for neurons and neurotransmitter action. (springer.com)
  • Western blot analysis of 14-3-3 gamma Antibody (Cat. (acris-antibodies.com)
  • Western blot analysis of extracts from K562 cells, treated with insulin 0.01U/ml 15', using 14-3-3 gamma Antibody. (acris-antibodies.com)
  • 14-3-3ζ Protein is suitable for use in Western Blot. (tebu-bio.com)
  • In addition, western blot analysis validated that the expression level of 1433ε was downregulated during curcumin‑induced apoptosis of MG‑63 cells compared with that in control cells. (spandidos-publications.com)
  • We analyzed CSF samples derived from 100 patients with various neurodegenerative disorders by Western blot of 14-3-3 protein, quantification of total tau (t-tau) protein, and phosphorylated tau (p-tau) protein. (springer.com)
  • The main result of our study was the validation of a commercially available 14-3-3 ELISA next to the commonly used Western blot method as a high-throughput screening test. (cdc.gov)
  • Průkaz proteinu 14-3-3 byl prováděn standardizovanou metodou western blot s chemiluminiscenční detekcí ß-podjednotky ve vzorku likvoru pacientů s podezřením na CJN. (csnn.eu)
  • An atypical form of bovine spongiform encephalopathy has been identified in cattle in Europe, North America and Japan and was designed as L-type BSE (L-BSE) due to the lower apparent molecular mass of the unglycosylated, protease-resistant prion protein (PrPres) detected by western blot compared with classical BSE. (blogspot.com)
  • Attenuation of the alpha2AR-14-3-3 interactions in the presence of a phosphorylated Raf-1 peptide corresponding to its 14-3-3 interacting domain (residues 251-266), but not by its non-phosphorylated counterpart, provides evidence for the functional specificity of these interactions and suggests one potential interface for the alpha2AR and 14-3-3 interactions. (rti.org)
  • In comparison to recent publications both sensitivity and specificity of 14-3-3 protein detection for CJD diagnosis is significantly lower in our patient group. (csnn.eu)
  • Because of its relatively low specificity it is important to consider the relevance of 14-3-3 positive test in each particular clinical case. (csnn.eu)
  • Assays for CSF 14-3-3 protein are probably moderately accurate in diagnosing sCJD: sensitivity 92% (95% confidence interval [CI] 89.8-93.6), specificity 80% (95% CI 77.4-83.0), likelihood ratio of 4.7, and negative likelihood ratio of 0.10. (blogspot.com)
  • In a prospective study of 110 patients done in The Netherlands (see reference below), it was found that the 14-3-3 assay was 97% sensitive and 87% specific. (blogspot.com)
  • Therefore, we concluded that the assay of t-tau protein may be useful as 1st screening and the ratio of p-tau protein/t-tau protein would be useful as 2nd screening to discriminate CJD from other neurodegenerative diseases. (springer.com)
  • In order to further improve the detection sensitivity of 14-3-3, a sandwich time-resolved fluoroimmunoassay (TRFIA) was developed using a pair of monoclonal antibodies, and it could be shown that TRFIA has a higher sensitivity in detecting 14-3-3 antigen of S. japonicum than an enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
  • Both alpha-SNAP and 14-3-3 proteins stimulated in a priming but not in a triggering assay. (rupress.org)
  • Blackledge, M. and Žídek, L.: Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. (findaphd.com)
  • Importantly, these activities are usually independent of substrate phosphorylation and therefore should be considered as distinct, 'moonlighting' functions of 14-3-3 proteins that mimic and complement the functions of dedicated molecular chaperones. (ovid.com)
  • Recent evidence that 14-3-3 proteins interfere with protein aggregate formation suggests they can also display a distinct, usually phosphorylation-independent, chaperone-like activity, which we review here as their 'moonlighting' function, which is of particular relevance in the context of neurodegenerative disorders. (ovid.com)
  • Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis. (rupress.org)
  • The effects of alpha-SNAP and 14-3-3 proteins were likely to be due to distinct mechanisms of action since they differed in Ca(2+)-dependency, time course and extent of stimulation and their effects were additive. (rupress.org)
  • The data show that these three proteins have distinct stage-specific actions on exocytosis and indicate that alpha-SNAP acts in an early MgATP-requiring stage and not in the late Ca(2+)-triggered steps immediately prior to membrane fusion as previously suggested. (rupress.org)
  • Urinary protein SDSPAGE analysis showed eight distinct bands in the urine of heartworm-infected dogs . (bvsalud.org)
  • Applying temperature-induced Triton X-114 phase separation experiments, they, as well as the target enzyme of fusicoccin action, the H(+)-ATPase, partitioned into the phospholipid-rich fraction which contains the most hydrophobic proteins. (nih.gov)
  • Fusicoccin, 14-3-3 proteins and defense responses in tomato plants. (lancs.ac.uk)
  • Fusicoccin, 14-3-3 proteins and defense respons. (lancs.ac.uk)
  • The p53 tumour-suppressor protein is a sequence-specific DNA-binding transcription factor that induces cell cycle arrest or apoptosis in response to genotoxic stress. (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • Several sequence analyses of these proteins have shown 4 regions with a high level of amino acid variability in loops I, V, VI, and VII (variable regions [VRs] 1-4) ( 2 ). (cdc.gov)
  • The most likely origin of the porB2/3 hybrid (4, Eb, 2ab, Cc) is the acquisition of DNA that encodes a VR1-4 sequence by a meningococcus with a porB2-99 allelic variant. (cdc.gov)
  • 14-3-3 binding may lead to cytoplasmic sequestration of the protein ligand but may also have other functional consequences. (nih.gov)
  • In response to the spatial cue provided by Aurora B, 14-3-3 promotes Ncd binding specifically to spindle microtubules by preventing it from binding other microtubules in the large cytoplasmic volume of oocytes. (rupress.org)
  • The arrow shows cytoplasmic localization of the N protein. (asm.org)
  • The membrane (lane2) and cytoplasmic (lane 3) fractions were immunoprecipitated with anti-Myc antibody. (asm.org)
  • Cytoplasmic (lane 2) and nuclear (lane 3) fractions were immunoprecipitated using anti-Myc antibody, resolved by 12% SDS-PAGE, and the protein bands were detected by fluorography. (asm.org)
  • Subsequently, the altered expression and localization of 1433ε and its co‑localization with other apoptosis‑associated proteins during apoptosis was investigated. (spandidos-publications.com)
  • The results of the present study revealed that 1433ε is an NMP in MG‑63 cells, and its altered expression and co‑localization with apoptosis‑associated proteins indicated an important function of 1433ε in apoptosis of MG‑63 cells. (spandidos-publications.com)
  • Grawe, Anissa P., "14-3-3 Protein Rad24 and its Effect on Mid1 Localization and Contractile Acto- Myosin Ring Assembly During Cytokinesis in S. pombe" (2018). (gvsu.edu)
  • 2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). (sinobiological.com)
  • Coordination of a client protein within the amphipathic groove of a 14-3-3 dimer can have a range of contextdependent effects such as conformational change, re-localization and bridging of two molecules. (uni-tuebingen.de)
  • PorB outer membrane proteins are transmembrane proteins with 8 predicted surface-exposed loops (I-VIII), which vary in length and in amino acid sequences. (cdc.gov)
  • Four proteins in this group, RGS6, RGS7, RGS9, and RGS11, share a common molecular organization of three modules: (i) the catalytic RGS domain, (ii) a GGL domain that recruits Gβ 5 , an outlying member of the G protein beta subunit family, and (iii) a DEP/DHEX domain that mediates interactions with the membrane anchor proteins R7BP and R9AP. (springer.com)
  • 90% of their protein constituents from the cytosol in a post-translational event. (plantcell.org)
  • In addition, the cells must retard folding of the organellar proteins before these polypeptides reach their final destination to avoid the biological function of the proteins in the wrong compartment, namely, the cytosol. (plantcell.org)
  • To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. (rti.org)
  • C) Van der Waals surface representation of one amphipathic 14-3-3 binding cleft showing the close contact of both ligands. (uni-tuebingen.de)
  • The restrained and the mutated (Arg56 or Arg129 to alanine) MD simulations indicate that the conformation of four residues (Lys49, Arg56, Arg129 and Tyr130) may play an important role to keep the 14-3-3σ protein in an open or closed state. (mdpi.com)
  • By binding in the 14-3-3 phosphopeptide binding groove, an amphipathic C-terminal helix of ExoS and ExoT has been thought to be crucial for their activation. (diva-portal.org)