Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Reference Standards: A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Linear Energy Transfer: Rate of energy dissipation along the path of charged particles. In radiobiology and health physics, exposure is measured in kiloelectron volts per micrometer of tissue (keV/micrometer T).Reference Values: The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Electrophoresis, Starch Gel: Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.Creutzfeldt-Jakob Syndrome: A rare transmissible encephalopathy most prevalent between the ages of 50 and 70 years. Affected individuals may present with sleep disturbances, personality changes, ATAXIA; APHASIA, visual loss, weakness, muscle atrophy, MYOCLONUS, progressive dementia, and death within one year of disease onset. A familial form exhibiting autosomal dominant inheritance and a new variant CJD (potentially associated with ENCEPHALOPATHY, BOVINE SPONGIFORM) have been described. Pathological features include prominent cerebellar and cerebral cortical spongiform degeneration and the presence of PRIONS. (From N Engl J Med, 1998 Dec 31;339(27))Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Chromatography, DEAE-Cellulose: A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Longevity: The normal length of time of an organism's life.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).ADP Ribose Transferases: Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.PhosphopeptidesBacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Adenosine Diphosphate Ribose: An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Plant Root Nodulation: The formation of a nitrogen-fixing cell mass on PLANT ROOTS following symbiotic infection by nitrogen-fixing bacteria such as RHIZOBIUM or FRANKIA.Schistosoma japonicum: A species of trematode blood flukes belonging to the family Schistosomatidae whose distribution is confined to areas of the Far East. The intermediate host is a snail. It occurs in man and other mammals.Bradyrhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria usually containing granules of poly-beta-hydroxybutyrate. They characteristically invade the root hairs of leguminous plants and act as intracellular symbionts.Schistosomiasis japonica: Schistosomiasis caused by Schistosoma japonicum. It is endemic in the Far East and affects the bowel, liver, and spleen.Apyrase: A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC 3.6.1.5.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Stroke: A group of pathological conditions characterized by sudden, non-convulsive loss of neurological function due to BRAIN ISCHEMIA or INTRACRANIAL HEMORRHAGES. Stroke is classified by the type of tissue NECROSIS, such as the anatomic location, vasculature involved, etiology, age of the affected individual, and hemorrhagic vs. non-hemorrhagic nature. (From Adams et al., Principles of Neurology, 6th ed, pp777-810)Astrocytes: A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.Brain Ischemia: Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.ReadingUp-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Cerebral Infarction: The formation of an area of NECROSIS in the CEREBRUM caused by an insufficiency of arterial or venous blood flow. Infarcts of the cerebrum are generally classified by hemisphere (i.e., left vs. right), lobe (e.g., frontal lobe infarction), arterial distribution (e.g., INFARCTION, ANTERIOR CEREBRAL ARTERY), and etiology (e.g., embolic infarction).
An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast. (1/1483)
The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well. (+info)Cell adhesion regulates the interaction between the docking protein p130(Cas) and the 14-3-3 proteins. (2/1483)
Integrin ligand binding induces a signaling complex formation via the direct association of the docking protein p130(Cas) (Cas) with diverse molecules. We report here that the 14-3-3zeta protein interacts with Cas in the yeast two-hybrid assay. We also found that the two proteins associate in mammalian cells and that this interaction takes place in a phosphoserine-dependent manner, because treatment of Cas with a serine phosphatase greatly reduced its ability to bind 14-3-3zeta. Furthermore, the Cas-14-3-3zeta interaction was found to be regulated by integrin-mediated cell adhesion. Thus, when cells are detached from the extracellular matrix, the binding of Cas to 14-3-3zeta is greatly diminished, whereas replating the cells onto fibronectin rapidly induces the association. Consistent with these results, we found that the subcellular localization of Cas and 14-3-3 is also regulated by integrin ligand binding and that the two proteins display a significant co-localization during cell attachment to the extracellular matrix. In conclusion, our results demonstrate that 14-3-3 proteins participate in integrin-activated signaling pathways through their interaction with Cas, which, in turn, may contribute to important biological responses regulated by cell adhesion to the extracellular matrix. (+info)Protein kinase C mu is negatively regulated by 14-3-3 signal transduction proteins. (3/1483)
Recent studies have documented direct interaction between 14-3-3 proteins and key molecules in signal transduction pathways like Ras, Cbl, and protein kinases. In T cells, the 14-3-3tau isoform has been shown to associate with protein kinase C theta and to negatively regulate interleukin-2 secretion. Here we present data that 14-3-3tau interacts with protein kinase C mu (PKCmu), a subtype that differs from other PKC members in structure and activation mechanisms. Specific interaction of PKCmu and 14-3-3tau can be shown in the T cell line Jurkat by immunocoprecipitiation and by pulldown assays of either endogenous or overexpressed proteins using PKCmu-specific antibodies and GST-14-3-3 fusion proteins, respectively. Using PKCmu deletion mutants, the 14-3-3tau binding region is mapped within the regulatory C1 domain. Binding of 14-3-3tau to PKCmu is significantly enhanced upon phorbol ester stimulation of PKCmu kinase activity in Jurkat cells and occurs via a Cbl-like serine containing consensus motif. However, 14-3-3tau is not a substrate of PKCmu. In contrast 14-3-3tau strongly down-regulates PKCmu kinase activity in vitro. Moreover, overexpression of 14-3-3tau significantly reduced phorbol ester induced activation of PKCmu kinase activity in intact cells. We therefore conclude that 14-3-3tau is a negative regulator of PKCmu in T cells. (+info)Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. (4/1483)
Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/ threonine kinase Akt, which then phosphorylates and inactivates components of the apoptotic machinery, including BAD and Caspase 9. In this study, we demonstrate that Akt also regulates the activity of FKHRL1, a member of the Forkhead family of transcription factors. In the presence of survival factors, Akt phosphorylates FKHRL1, leading to FKHRL1's association with 14-3-3 proteins and FKHRL1's retention in the cytoplasm. Survival factor withdrawal leads to FKHRL1 dephosphorylation, nuclear translocation, and target gene activation. Within the nucleus, FKHRL1 triggers apoptosis most likely by inducing the expression of genes that are critical for cell death, such as the Fas ligand gene. (+info)Ca2+-induced apoptosis through calcineurin dephosphorylation of BAD. (5/1483)
The Ca2+-activated protein phosphatase calcineurin induces apoptosis, but the mechanism is unknown. Calcineurin was found to dephosphorylate BAD, a pro-apoptotic member of the Bcl-2 family, thus enhancing BAD heterodimerization with Bcl-xL and promoting apoptosis. The Ca2+-induced dephosphorylation of BAD correlated with its dissociation from 14-3-3 in the cytosol and translocation to mitochondria where Bcl-xL resides. In hippocampal neurons, L-glutamate, an inducer of Ca2+ influx and calcineurin activation, triggered mitochondrial targeting of BAD and apoptosis, which were both suppressible by coexpression of a dominant-inhibitory mutant of calcineurin or pharmacological inhibitors of this phosphatase. Thus, a Ca2+-inducible mechanism for apoptosis induction operates by regulating BAD phosphorylation and localization in cells. (+info)Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants. (6/1483)
Fusicoccin (FC) is a fungal toxin that activates the plant plasma membrane H+-ATPase by binding with 14-3-3 proteins, causing membrane hyperpolarization. Here we report on the effect of FC on a gene-for-gene pathogen-resistance response and show that FC application induces the expression of several genes involved in plant responses to pathogens. Ten members of the FC-binding 14-3-3 protein gene family were isolated from tomato (Lycopersicon esculentum) to characterize their role in defense responses. Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. Different specific subsets of 14-3-3 genes were induced after treatment with FC and during a gene-for-gene resistance response. Possible roles for the H+-ATPase and 14-3-3 proteins in responses to pathogens are discussed. (+info)Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import. (7/1483)
Cdc2-cyclin B1 in the G2-arrested Xenopus oocyte is held inactive by phosphorylation of Cdc2 at two negative regulatory sites, Thr14 and Tyr15. Upon treatment with progesterone, these sites are dephosphorylated by the dual specificity phosphatase, Cdc25, leading to Cdc2-cyclin B1 activation. Whereas maintenance of the G2 arrest depends upon preventing Cdc25-induced Cdc2 dephosphorylation, the mechanisms responsible for keeping Cdc25 in check in these cells have not yet been described. Here we report that Cdc25 in the G2-arrested oocyte is bound to 14-3-3 proteins and that progesterone treatment abrogates this binding. We demonstrate that Cdc25, apparently statically localized in the cytoplasm, is actually capable of shuttling in and out of the oocyte nucleus. Binding of 14-3-3 protein markedly reduces the nuclear import rate of Cdc25, allowing nuclear export mediated by a nuclear export sequence present in the N-terminus of Cdc25 to predominate. If 14-3-3 binding to Cdc25 is prevented while nuclear export is inhibited, the coordinate nuclear accumulation of Cdc25 and Cdc2-cyclin B1 facilitates their mutual activation, thereby promoting oocyte maturation. (+info)The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2-adrenergic receptor subtypes. (8/1483)
The alpha2-adrenergic receptors (alpha2ARs) are localized to and function on the basolateral surface in polarized renal epithelial cells via a mechanism involving the third cytoplasmic loop. To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. A protein doublet of approximately 30 kDa in Madin-Darby canine kidney cells or pig brain cytosol (alpha2B >/= alpha2C>> alpha2A) was identified. The interacting protein was purified by sequential DEAE and size exclusion chromatography, and subsequent microsequencing revealed that they are the zeta isoform of 14-3-3 proteins. [35S]Met-14-3-3zeta binds to all three native alpha2AR subtypes, assessed using a solid phase binding assay (alpha2A>/=alpha2B> alpha2C), and this binding depends on the presence of the 3i loops. Attenuation of the alpha2AR-14-3-3 interactions in the presence of a phosphorylated Raf-1 peptide corresponding to its 14-3-3 interacting domain (residues 251-266), but not by its non-phosphorylated counterpart, provides evidence for the functional specificity of these interactions and suggests one potential interface for the alpha2AR and 14-3-3 interactions. These studies represent the first evidence for G protein-coupled receptor interactions with 14-3-3 proteins and may provide a mechanism for receptor localization and/or coordination of signal transduction. (+info)"Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins". Proteins. 65 (3): 643-55. doi: ... Notably, the protein contains three EF hand motifs that function as binding sites for Ca2+ ions. The protein is a member of the ... In the animals, the protein occurs together with the Green fluorescent protein to produce green light by resonant energy ... Since then, the protein has been widely used as reported in many model biological systems, including zebrafish, rats, mice, and ...
Protein Science. 14 (3): 812-822. doi:10.1110/ps.04951405. ISSN 0961-8368. PMC 2279270 . PMID 15722453. Hulver MW, Berggren JR ... "Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B ... "Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B ... 3 (4): 271-80. doi:10.2174/1568008033340117. PMID 14683458. Shiwaku K, Hashimoto M, Kitajima K, Nogi A, Anuurad E, Enkhmaa B, ...
Hydrogen bonds are (red) between protein, water (blue balls) and the cofactor PLP (purple). Figure 4 shows the alpha helices ( ... Koyata H, Hiraga K (1991). "The glycine cleavage system: Structure of a cDNA encoding human H-protein, and partial ... Protein Science. 14 (3): 791-8. doi:10.1110/ps.041179105. PMC 2279282 . PMID 15689518. "KEGG ENZYME Database Entry". Kyoto ... its activity is augmented by high-protein diets and starvation. During periods of low carbohydrates, serine is converted into ...
Users can search the database for a particular motion by either protein name or Protein Data Bank ID number. Typically, however ... ISBN 0-470-18105-2. "Chapter 26 on Protein Motions". The Physics of Proteins: An Introduction to Biological Physics and ... users will enter the database via the Protein Data Bank, which often provides a hyperlink to the molmovdb entry for proteins ... Database of protein conformational diversity Gu, Jenny; Bourne, Philip E. (March 2009). Structural Bioinformatics (2nd ed.). ...
protein binding. • MHC class II protein complex binding. • protein heterodimerization activity. • enzyme binding. • poly(A) RNA ... identical protein binding. • macromolecular complex binding. • cadherin binding. • scaffold protein binding. Cellular component ... protein localization to nucleus. • cellular response to heat. • positive regulation of protein export from nucleus. • ... protein domain specific binding. • protein complex binding. • histone deacetylase binding. • potassium channel regulator ...
The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has ... Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53-63. doi ... "Protein kinase CK2 regulates CDC25B phosphatase activity". Oncogene. England. 22 (2): 220-32. doi:10.1038/sj.onc.1206107. ISSN ... 14 (20): 2485-95. doi:10.1038/sj.onc.1201063. PMID 9188863. Booher RN, Holman PS, Fattaey A (1997). "Human Myt1 is a cell cycle ...
Forkhead box O6 is a protein that in humans is encoded by the FOXO6 gene. GRCm38: Ensembl release 89: ENSMUSG00000052135 - ... 5 (3): 233-40. doi:10.1111/1753-0407.12027. PMC 3657578 . PMID 23324123. Qinyu L, Long C, Zhen-dong D, Min-min S, Wei-ze W, Wei ... Tzivion G, Dobson M, Ramakrishnan G (November 2011). "FoxO transcription factors; Regulation by AKT and 14-3-3 proteins". ...
M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A is a protein that in humans is encoded by the ... In contrast, some invertebrates harbour 2 (e.g., the Drosophila proteins String and Twine) or four (e.g., C. elegans Cdc-25.1 ... Dual-specificity protein phosphatases remove phosphate groups from phosphorylated tyrosine and serine / threonine residues. ... Coqueret O, Bérubé G, Nepveu A (1998). "The mammalian Cut homeodomain protein functions as a cell-cycle-dependent ...
Raf kinases A-Raf kinase B-Raf kinase KSR1 protein KSR2 protein GRCm38: Ensembl release 89: ENSMUSG00000000441 - Ensembl, May ... Yuryev A, Wennogle LP (February 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid ... a novel jun N-terminal protein kinase (JNK)-binding protein that functions as a Scaffold factor in the JNK signaling pathway". ... and the enzymes dedicated for removal of these phosphates are the protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A ...
Mitogen-activated protein kinase kinase kinase 2 is an enzyme that in humans is encoded by the MAP3K2 gene. The protein encoded ... Cheng J, Zhang D, Kim K, Zhao Y, Zhao Y, Su B (Jul 2005). "Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and ... This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a ... Yan M, Dai T, Deak JC, Kyriakis JM, Zon LI, Woodgett JR, Templeton DJ (1995). "Activation of stress-activated protein kinase by ...
"A helix-loop-helix protein related to the immunoglobulin E box-binding proteins". Molecular and Cellular Biology. 10 (8): 4384- ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ... Transcription factor EB is a protein that in humans is encoded by the TFEB gene.[5][6] ... 21] TFEB nuclear export is mediated by CRM1 and is dependent on phosphorylation.[22][23] TFEB is also a target of the protein ...
One such protein is rad18 that is required for G2 arrest even when chk1 is phosphorylated and active. This protein is also ... The cell cycle is driven by proteins called cyclin dependent kinases that associate with cyclin regulatory proteins at ... Proteins that localize to sites of DNA damage in the G2 phase initiate a signaling cascade that regulates wee1 and cdc25 ... Proteins that function in the G2-M checkpoint were originally identified in yeast screens that looked for mutants which show ...
... protein, human at the US National Library of Medicine Medical Subject Headings (MeSH). ... or K2P18.1 is a protein that in humans is encoded by the KCNK18 gene. K2P18.1 is a potassium channel containing two pore- ... Czirják G, Vuity D, Enyedi P (2008). "Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation". J. Biol ...
The protein can be a toxin, an apoptotic factor, or a viral protein. Toxins such as diphtheria toxin interfere with cellular ... "Regulation of telomerase activity and anti-apoptotic function by protein-protein interaction and phosphorylation". FEBS Lett. ... Viral proteins like viral thymidine kinase can be used for specific targeting of a drug. By introducing a prodrug only ... Song H, Li Y, Chen G, Xing Z, Zhao J, Yokoyama KK, Li T, Zhao M (April 2004). "Human MCRS2, a cell-cycle-dependent protein, ...
... camp-responsive element-binding protein (CREB) and CREB-binding protein (CBP). It has been proposed that recruitment of ... Mutant atrophin-1 proteins have been found in neuronal intranuclear inclusions (NII) and diffusely accumulated in the neuronal ... Atrophin-1 may be a dispensable or redundant protein as mice bred with a null allele for atrophin-1 produce viable and fertile ... It contains a putative nuclear localization signal in the N-terminus of the protein and a putative nuclear export signal in the ...
This protein family is known for regulating signal transduction pathways primarily through binding phosphoserine proteins, ... is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. The protein encoded by this gene is a member of the ... though it can also bind phosphothreonine proteins and unphosphorylated proteins. By extension, 14-3-3 proteins are involved in ... "New insights into potential functions for the protein 4.1 superfamily of proteins in kidney epithelium". Frontiers in ...
T-cell leukemia homeobox protein 2 is a protein that in humans is encoded by the TLX2 gene. TLX2 has been shown to interact ... 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMC 528928 . PMID 15489334. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ... Tang SJ, Suen TC, McInnes RR, Buchwald M (1998). "Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins". J. ... "Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins". J. Biol. Chem. UNITED STATES. 273 (39): 25356-63. doi: ...
ER retention refers to proteins that are retained in the endoplasmic reticulum, or ER, after folding; these are known as ER ... The classical ER retention signal is the C-terminal KDEL sequence for lumen bound proteins and KKXX for transmembrane ... These signals allow for retrieval from the Golgi apparatus by ER retention receptors, effectively maintaining the protein in ... resident proteins. Their localization to the ER often depends on certain sequences of amino acids located at the N-terminus or ...
The cause was determined to be a histidine-to-tyrosine (amino acid) substitution at position 274 in its neuraminidase protein. ... A single hemagglutinin-neuraminidase protein can combine neuraminidase and hemagglutinin functions, such as in mumps virus and ... Neuraminidase also cleaves sialic acid residues from viral proteins, preventing aggregation of viruses. Neuraminidase has been ... Proteins: Structure, Function, and Genetics. 14 (3): 327-32. doi:10.1002/prot.340140302. PMID 1438172. Huang IC, Li W, Sui J, ...
Anson ML, Protein Denaturation and the Properties of Protein Groups, Advances in Protein Chemistry, 2, 361-386 (1945) ... Protein Sci. 4(10), 2138-2148 (1995) Robertson, A.D., Murphy, K.P. Protein structure and the energetics of protein stability, ( ... 1992) "Physical Basis for the Stability of the Folded Conformations of Proteins", in Protein Folding, TE Creighton, ed., W. H. ... "The use of high-pressure nuclear magnetic resonance to study protein folding". In Bai, Yawen; Nussinov, Ruth. Protein folding ...
... is a mammalian gene encoding the protein CBL which is an E3 ubiquitin-protein ligase involved in cell signalling and protein ... "The Cbl proteins are binding partners for the Cool/Pix family of p21-activated kinase-binding proteins". FEBS Lett. 550 (1-3): ... "The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src ... "The protein product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the ...
... the protein import tunnel Toc75, plus the proteins Toc64 and Toc12. The first three proteins form a core complex that consists ... A protein kinase drifting around on the outer chloroplast membrane can use ATP to add a phosphate group to the Toc34 protein, ... This loss of GTP makes the Toc34 protein release the chloroplast preprotein, handing it off to the next TOC protein. Toc34 then ... Hundreds of different PPR proteins from the nuclear genome are involved in the RNA editing process. These proteins consist of ...
Mutant p53 proteins often fail to induce MDM2, causing p53 to accumulate at very high levels. Moreover, the mutant p53 protein ... In addition to the full-length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to ... One such example, human papillomavirus (HPV), encodes a protein, E6, which binds to the p53 protein and inactivates it. This ... The full length p53 isoform proteins can be subdivided into different protein domains. Starting from the N-terminus, there are ...
... allowing for Rab protein to change from its GDP to GTP bound state. Inhibition of the GTPase-activating domain leaves proteins ... Like all proteins, the unique amino acid arrangement in the primary sequence of GLUT4 are what allow it to transport glucose ... Two proteins with capacity to bind Ubc9 and conjugated to SUMO1". The Journal of Biological Chemistry. 277 (22): 19783-91. doi: ... GLUT4 Protein at the US National Library of Medicine Medical Subject Headings (MeSH) USCD-Nature molecule pages: The signaling ...
The alpha-2B adrenergic receptor (α2B adrenoceptor), is a G-protein coupled receptor. It is a subtype of the adrenergic ... This gene encodes the α2B subtype, which was observed to associate with eIF-2B, a guanine nucleotide exchange protein that ... Comparative analysis of the primary protein sequence of ADRA2B across placentals also showed the high conservation of residues ... "Regulation of adenylyl cyclase in polarized renal epithelial cells by G protein-coupled receptors". The American Journal of ...
... is a subjective symptom of fatigue that is experienced by nearly all cancer patients. Among patients receiving cancer treatment other than surgery, it is essentially universal. Fatigue is a normal and expected side effect of most forms of chemotherapy, radiation therapy, and biotherapy. On average, cancer-related fatigue is "more severe, more distressing, and less likely to be relieved by rest" than fatigue experienced by healthy people. It can range from mild to severe, and may be either temporary or a long-term effect. Fatigue may be a symptom of the cancer, or it may be the result of treatments for the cancer. The National Comprehensive Cancer Network defines cancer-related fatigue as "a distressing persistent, subjective sense of physical, emotional and/or cognitive tiredness or exhaustion related to cancer or cancer treatment that is not proportional to recent activity and interferes with usual functioning". Cancer-related fatigue is a chronic fatigue (persistent ...
... is intended to reduce fatigue related fatalities and incidents. Several companies are working on a technology for use in industries such as mining, road- and rail haulage and aviation. The technology may soon find wider applications in industries such as health care and education. In an operational environment scenario where operating systems are dependent on human performance, fatigue can be defined as an inclination to degrade performance. Thus, fatigue is an indicator of baseline risk for the occurrence of errors and accidents. Globally mining operations are at risk of fatigued workers. Sleepiness and fatigue increase human error and contribute to accidents which can be fatal. Factors compounding fatigue levels in mine workers include; disruptions in circadian rhythms due to shift work, exposure to noise, vibration and chemicals, monotonous and repetitive nature of tasks and night shift driving. Studies recognise a connotation between lifestyle and fatigue. Mine ...
... , or central fatigue, is a form of fatigue that is associated with changes in the synaptic concentration of neurotransmitters within the central nervous system (CNS; including the brain and spinal cord) which affects exercise performance and muscle function and cannot be explained by peripheral factors that affect muscle function. In healthy individuals, central fatigue can occur from prolonged exercise and is associated with neurochemical changes in the brain, primarily involving serotonin (5-HT), noradrenaline, and dopamine. Central fatigue plays an important role in endurance sports and also highlights the importance of proper nutrition in endurance athletes. Existing experimental methods have provided enough evidence to suggest that variations in synaptic serotonin, noradrenaline, and dopamine are significant drivers of central nervous system fatigue. An increased synaptic dopamine concentration in the CNS is strongly ergogenic (promotes exercise performance). ...
ME happens when a low-melting-point metal is placed in contact with a metal with higher melting point, so the latter is embrittled. It is often manifested by static fatigue. For example, as shown in Figure 1,[2] in a test of the static fatigue of a 2024 aluminum coated with mercury, the alloy is subject to a stress level less than the value causing plastic flow, and the time it takes to fracture is measured. Usually a stress called static fatigue limit is present, representing the boundary below which the material does not fracture, no matter how long the test duration is. In this scenario, the static fatigue often depends on the presence of initial flaws. Also, if the material is "flaw-free", its static fatigue limit serves as a design parameter in a hostile environment. ...
Modern technology seeks to minimize or prevent listener fatigue entirely. Blockage of the ear canal, common in [headphones], is thought to be a main contributing factor in listener fatigue. When cut off from outside sound with the earphone, an oscillating pressure chamber is created in the eardrum. This effectively provides a boost in sound pressure levels. When this boost occurs, an acoustic reflex mechanism triggers and acts as a defense against these sounds. This mechanism seeks to reduce the sound energy in the ear by dampening its transfer from eardrum to cochlea. It has been seen that this process can reduce sound waves by up to 50 decibels. Although this mechanism can decrease the sound energy, it does not negate the oscillatory pressure. Due to this defense mechanism, sounds do not seem as loud as they are, and ironically, listeners will want to increase the volume. As a result, the reflex mechanism is activated again, and the cycle continues on. This ultimately leads to fatigue. ...
The second phase of Ranger School is conducted at the remote Camp Merrill near Dahlonega, Georgia by the 5th Ranger Training Battalion. Here, "students receive instruction on military mountaineering tasks, mobility training, as well as techniques for employing a platoon for continuous combat patrol operations in a mountainous environment".[20] Adding to the physical hardships endured in the Benning phase, in this phase "the stamina and commitment of the Ranger student is stressed to the maximum. At any time, he or she may be selected to lead tired, hungry, physically expended students to accomplish yet another combat patrol mission".[20] The Ranger student continues learning how to sustain themselves and their subordinates in the mountains. The rugged terrain, severe weather, hunger, mental and physical fatigue, and the psychological stress the student encounters allow them to measure their capabilities and limitations and those of their fellow soldiers. In addition to combat operations, the ...
Nerves are responsible for controlling the contraction of muscles, determining the number, sequence and force of muscular contraction. Most movements require a force far below what a muscle could potentially generate, and barring pathological nervous fatigue, is seldom an issue[vague][clarification needed]. But in extremely powerful contractions that are close to the upper limit of a muscle's ability to generate force, nervous fatigue (enervation), in which the nerve signal weakens, can be a limiting factor in untrained individuals. In novice strength trainers, the muscle's ability to generate force is most strongly limited by nerve's ability to sustain a high-frequency signal. After a period of maximum contraction, the nerve's signal reduces in frequency and the force generated by the contraction diminishes. There is no sensation of pain or discomfort, the muscle appears to simply 'stop listening' and gradually cease to move, often going backwards. As there is insufficient stress on the muscles ...
In June 2017, the U.S. Centers for Disease Control and Prevention stated that speaking with a therapist may help.[88] Previously, a 2014 National Institutes of Health report concluded that while counseling and behavior therapies could produce benefits for some people, they may not yield improvement in quality of life, and because of this limitation such therapies should not be considered as a primary treatment, but rather should be used only as one component of a broader approach.[89] This same report stated that although counseling approaches have shown benefit in some measures of fatigue, function and overall improvement, these approaches have been inadequately studied in subgroups of the wider CFS patient population. Further concern was expressed that reporting of negative effects experienced by patients receiving counseling and behavior therapies had been poor.[86] A report by the Institute of Medicine published in 2015 states that it is unclear whether CBT helps to improve cognitive ...
Metastaasiks ehk siirdeks ehk teiseseks haiguskoldeks (ladina metastasis) on mitmetel loomadel esineda võiv metastaatiline haiguslik seisund. Metastaasidele on iseloomulik pahaloomuliste kasvajarakkude, mikroobide levik algkoldest, peamiselt vereringe ja lümfisõlmede ning lümfisüsteemi vahendusel, organismi teistesse elunditesse. Peamiselt peetakse metastaaside puhul silmas siiski pahaloomulise kasvaja algkoldest väljaspool paikneva teisese koldega seonduvat. Kõik pahaloomulised kasvajad võivad metastaseeruda. Kui kasvaja rakk ja/või rakud erinevate tegurite kaasabil metastaseeruvad ja teises elundis paljunevad ning moodustavad seal veresoonkonnavälise kasvava massi, siis nimetatakse uut kasvajakollet rakkude sarnasuse tõttu teiseseks kasvajakoldeks. Metastaaside tavalised paikmed on luud, peaaju, diafragma, süda, maks, kops, lümfisõlmed, keskseinand, ajukelmed, kõhukelme, pleura, retroperitoneum, seljaaju.[1]. Üksikute kasvajarakkude levikut ehk mikrometastaase pole võimalik ...
Hodgkini lümfoomi levinuimad sümptomid on kaela, kaenlaaluse või vaagna piirkonnas paikneva ühe või mitme lümfisõlme, harilikult valutu, suurenemine. Lümfoomi korral suurenevad lümfisõlmed aeglaselt ja võib kuluda kuid või aastaid, enne kui neid märgatakse. Mõnikord võivad lümfisõlmed suureneda väga kiiresti.[11] Rohkem kui 80%-l patsientidest on leitud lümfadenopaatia ülalpool diafragmat.[2] Kolmandikul (25%) patsientidest esineb Hodgkini lümfoomi korral ka üldsümptomeid (nn B-sümptomid), näiteks öine higistamine, kõrge kehatemperatuur, mis tõuseb ja alaneb spontaanselt, märgatav kaalukaotus lühikese aja jooksul, sügelemine, mis võib pärast alkoholi seespidist tarbimist intensiivistuda, köha või hingeldus, kõhuvalu või oksendamine pärast alkoholi joomist.[11] ...
Interleukiin-10 ehk IL-10 on paljude selgroogsete loomade mitmete lümfisüsteemi rakkude poolt teatud sündmuste korral vabastatavad endogeensed lühiajalised valgulised signaalmolekulid, mis vahendavad tõenäoliselt põletikuvastaseid jaimmunosupressiivseid toimeid (immuunvastuse pidurdamine) ning mis liigitatakse põletikuvastaste tsütokiinide hulka. IL-10-t toodavad ja vabastavad T- (CD4 T-rakud, CD8 T-rakud jt), B-rakud, monotsüüdid, dendriitrakud ja makrofaagid, aga ka kesknärvisüsteemi liigitatud gliiarakud jpt. Eri tüüpi tsütokiinid kasutavad nn segasignaale, nii näiteks inhibeerib IL-4 ja IL-10 eritamine Th1- tüüpi immuunvastuste supressiooni, vähendamaks makrofaagidelt tuleva IL-12 tootmist. Põletikutoime arvatakse seisnevat põletikutsütokiinide, nagu IFN-γ, IL-2, IL-3, TNFα ja GM-CSF, sünteesi inhibeerimises. Interleukiin-10 on 2. rühma (IL10- perekonna), mis koosneb interleukiin-10, IL-19, IL-20, IL-22, IL-24 (Mda-7) ja IL-26, interferoonidest (IFN-alfa, -beeta, ...
TIL-rakud ehk tuumorikoe lümfotsüüdid ehk kasvajasse infiltreeruvad lümfotsüüdid (inglise tumor-infiltrating lymphocyte) on osade selgroogsete uudismoodustistes funktsioneeriv rakutüüp.[1] Osadesse kasvajatesse infiltreeruvad TIL-rakud loetakse lümfisüsteemi (lümfoid(-immuun)süsteemi) rakupopulatsioonide - leukotsüütide hulka ja neile omistatakse osa organismi kasvajavastases immuunvastuses. Arvatakse, et TIL-rakud on püsivad kasvajarakkude kaaslased ja nende osa seostatakse enim kasvajarakkude hävitamisega. ...
Roomavat madarat kasutatakse ravimtaimena. Ürt (Galii aparinis herva) on taime maapealne osa. Ürti kogutakse taime õitsemise ajal ja kuivatatakse. Droog sisaldab iridoide, flavonoide, antratseene, kumariine, tanniine, orgaanilisi happeid, mineraalaineid (vask, tsink, raud, mangaan, boor, titaan, strontsium, vanaadium, tallium, nikkel, molübdeen), rasvõli jpm.[4] Droogil on uriinieritust suurendav toime. Kasutatakse düsuuria, kuseteede, lümfisõlme-, mao- ja peensoolepõletiku, haavandtõve ja palaviku puhul. Suurendab sapinõristust.[4] Rahvameditsiinis peetakse teda põletiku-, mikroobi-, oksüdeerumis- ja vähivastaseks.[6] ...
Proportion of tau protein to phosphorylated tau protein CSF levels in differential diagnosis of dementia. Nervenarzt 2008; 79(8 ... Protein 14-3-3 Detection in Cerebrospinal Fluid - Clinico-Pathological Correlation Introduction:. The 14-3-3 - protein group is ... Protein 14-3-3 detection was performed by a standardized method using western blot with chemoluminiscent detection of ß-subunit ... Protein 14-3-3 byl poprvé popsán Moorem a Pertezem v roce 1967 [1] jako významná kyselá frakce mozkových proteinů. Skupinu ...
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... 14-3-3 protein homolog. Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast). Loading... ... 14-3-3-like protein. Oenothera elata subsp. hookeri (Hookers evening primrose). Loading... ...
2006 Jun;16(3):183-92. Epub 2006 Apr 1. Research Support, Non-U.S. Govt; Review ... M-transition by binding to regulatory proteins and modulating their function. In most cases, the association with 14-3-3 ... proteins requires a specific phosphorylation of the protein ligand and mediates cell cycle arrest. 14-3-3 binding may lead to ... Effects of 14-3-3 proteins on cell cycle progression and the regulation of 14-3-3 activity during the cell cycle are reviewed ...
The 14-3-3 proteins are binding proteins that have been shown to interact with a wide array of enzymes involved in primary ... In this review, we examine the factors that may regulate the binding of 14-3-3s to their target proteins, and discuss their ... In most cases, the significance of binding of the 14-3-3 protein is not known. However, most of the interactions are ... Metabolic enzymes as targets for 14-3-3 proteins.. Huber SC1, MacKintosh C, Kaiser WM. ...
... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... of PC12 proteins and identified the TH protein as a major constituent of the proteins that are co-immunoprecipitated with 14-3- ... 5 cytoskelton proteins, 18 nuclear proteins including transcription factors, 10 metabolic enzymes, and others. We previously ...
... is part of an EU Framework IV Biotech-programme entitled Central Role in Adaptation of Fourteen Three Three proteins, and is ... 6, 341-347) by serving as docking proteins for receptors, kinases and phosphatases. In plants some 14-3-3 isoforms make up the ... translocation within the cell and docking to the respective targets proteins) will be studied by means of Green Fuorescent ... Candidates should have demonstrated experience and publications in biochemistry/protein chemistry and molecular biology. ...
Reversible protein phosphorylation events are known to play a crucial role in the regulation of stomatal movements. However, in ... Among the phosphopeptide-binding proteins, the 14-3-3 proteins are the best characterized in plants. The 14-3-3s are found as ... Among the phosphopeptide-binding proteins, the 14-3-3 proteins are the best characterized in plants. The 14-3-3s are found as ... However, in some cases, phosphorylation alone is not sufficient to achieve complete protein regulation, but is necessary to ...
The Bmh2 protein is , 70% identical to the mammalian epsilon-isoform and , 90% identical to a previously reported yeast 14-3-3 ... Now we report that one of the suppressor loci, BMH2/SCD3, encodes a protein of the 14-3-3 family. The 14-3-3 proteins are ... encoding a cAMP-dependent protein kinase, restores Bmh- yeast to viability. High-copy TPK1 did not rescue Chc- yeast. These ... protein encoded by BMH1. Single deletions of BMH1 or BMH2 have no discernable phenotypes, but deletion of both BMH1 and BMH2 is ...
We suggest that the target protein is attracted first by general protein-protein interaction motifs (the desolvation patches) ... Structural basis for protein-protein interactions in the 14-3-3 protein family. Xiaowen Yang, Wen Hwa Lee, Frank Sobott, ... Approximately 2 μl of protein solution (10-30 μM) was loaded per capillary. Protein samples were buffer exchanged into 20 mM ... 4, where red patches represent likely protein-protein interaction interfaces. No low-desolvation patches were observed for any ...
14-3-3 zeta/delta Proteins available through Novus Biologicals. Browse our 14-3-3 zeta/delta Protein catalog backed by our ... MGC111427 protein, MGC138156 protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide ... Protein Array, SDS-Page, Western Blot. Each 14-3-3 zeta/delta Peptide and 14-3-3 zeta/delta Protein is fully covered by our ... Our 14-3-3 zeta/delta Peptides and 14-3-3 zeta/delta Proteins can be used in a variety of model species: Human. Use the list ...
The p53 tumour-suppressor protein is a sequence-specific DNA-binding transcription factor that induces cell cycle arrest or ... Both the levels of p53 protein and its affinity for specific DNA sequences increase in response to genotoxic stress. In vitro, ... The p53 tumour-suppressor protein is a sequence-specific DNA-binding transcription factor that induces cell cycle arrest or ... ATM-dependent activation of p53 involves dephosphorylation and association with 14-3-3 proteins Nat Genet. 1998 Jun;19(2):175-8 ...
A Number of Functions for a Numbered Protein ... A Number of Functions for a Numbered Protein ... A Number of Functions for a Numbered Protein ... A Number of Functions for a Numbered Protein ...
References for Abcams Recombinant Human 14-3-3 eta protein (ab116442). Please let us know if you have used this product in ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
Ab40510 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam provides free ... Protein kinase C inhibitor protein 1. *Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex Assays. By ... Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... This protein in other organisms (by gene name): Q2QBT8 - Giardia intestinalis 2 * A8BJ73 - Giardia intestinalis no matching PDB ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... The Protein Feature View requires a browser that supports SVG (Scalable Vector Graphics). Mouse over tracks and labels for more ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Protein Feature View of PDB entries mapped to a UniProtKB sequence * Number of PDB entries for Q6P3U9: no matching PDB entries ... This protein in other organisms (by gene name): Q6P3U9 - Homo sapiens 0 * Q32P43 - Homo sapiens no matching PDB entries ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ...
Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). ... Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... "Structural basis for protein-protein interactions in the 14-3-3 protein family.". Yang X., Lee W.H., Sobott F., Papagrigoriou E ...
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... "Structural basis for protein-protein interactions in the 14-3-3 protein family.". Yang X., Lee W.H., Sobott F., Papagrigoriou E ... PROSITE; a protein domain and family database. More...PROSITEi. View protein in PROSITE. PS00796 1433_1, 1 hit. PS00797 1433_ ...
Members of a family of highly conserved proteins, termed 14-3-3 proteins, were found by several experimental approaches to ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins ... Optimal complex formation required the amino-terminal regulatory domain of Raf-1. The association of 14-3-3 proteins and Raf-1 ...
apoptosis, ASKI, Bad, difopein, PKB, protein kinase B, ASKI, apoptosis signal-regulating kinase I, EYFP, enhanced yellow ... domain in the regulation of apoptosis and Bcl-2-related proteins Biochem Soc Trans (February, 2000) ... Survival-promoting functions of 14-3-3 proteins S. C. Masters; S. C. Masters ... Thus, 14-3-3 may be part of the cellular machinery that maintains cell survival, and targeting 14-3-3-ligand interactions may ...
The proteins were N-terminally blocked. Internal amino acid sequences were obtained for both polypeptides of the fusicoccin- ... which comprises structurally conserved regulatory proteins of widespread occurrence and a wide range of functions. 14-3-3 ... partitioned into the phospholipid-rich fraction which contains the most hydrophobic proteins. The results discussed herein ... The fusicoccin receptor of plants is a member of the 14-3-3 superfamily of eukaryotic regulatory proteins FEBS Lett. 1994 Sep ...
These proteins contain a number of known common modification domains, including regions for divalent cation interaction, ... More than 200 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and ... To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain.[ ... They are believed to interact with the protein Activation-Induced (Cytidine) Deaminase in mediating class switch recombination ...
... about 14-3-3 PROTEINS. Search and download thousands of Swedish university dissertations. Full text. Free. ... Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein-protein interaction; GUS; Arabidopsis; 14-3-3; H -ATPase; expression; ... To date, 104 proteins have been shown to interact with 14-3-3s of which 85 are found in mammals and yeast, and 21 are found in ... Abstract : The P-type plasma membrane H+-ATPases form a group of proteins only found in plants and fungi. The pumping of ...
... proteopedia.org/wiki/index.php/14-3-3_protein_3D_structures" ... 14-3-3 protein 3D structures. From Proteopedia. Jump to: ... 3D structures of 14-3-3 protein. Updated on 25-May-2020 ...
Role of acidic chromatin proteins in high-affinity binding Biochem J (May,1976) ... Multiple roles for 14-3-3 proteins in nucleo-cytoplasmic transport A. Brunet; A. Brunet ... A. Brunet, F. Kanai, J. Stehn, M. E. Greenberg, M. B. Yaffe; Multiple roles for 14-3-3 proteins in nucleo-cytoplasmic transport ... Biochem Soc Trans 1 June 2002; 30 (3): A64. doi: https://doi.org/10.1042/bst030a064a ...
CerebrospinalProteinuSensitivityDetectionClinicalZetaRegulationEukaryoticRegulateAbstractIsoformTyrosineFound that phosphodockMotifsInteractions with 14-3-3 protAssociation with 14-3-3 protPlant 14-3-3 protRolesPathwaysPeptidesPhosphorylation-dependentArabidopsisCerebrospinal fluidBindsBarleySubcellular LocalizationWestern Blot2002PutativeSigmaPlasma membraneSpecificityPhosphoserineEndothelial CellsHighly conserved and ubiquitously expressedMitogen-activaAssayTargetsDistinctFusicoccinIdentical to the mouse orthologSequenceIntracellularAcidic proteinsFamily of proteinsCytoplasmicLocalizationMembraneMolecularEncodesPlantsCytosolRecombinantFunctionallyLigandsResiduesApoptosis signal-regulaPhosphopeptide bindin
- Detection of the ß-subunit of the 14-3-3 protein in cerebrospinal fluid is one of the most important paraclinical markers in sporadic Creutzfeldt-Jakob disease (sCJD) diagnostic procedure. (csnn.eu)
- Protein 14-3-3 detection was performed by a standardized method using western blot with chemoluminiscent detection of ß-subunit in cerebrospinal fluid samples from patients with clinical suspection of CJD. (csnn.eu)
- 57 cerebrospinal fluid samples were 14-3-3 - positive and 20 were weakly positive from all 280 samples in total. (csnn.eu)
- Nevertheless, a positive finding of protein 14-3-3 in the cerebrospinal fluid is one of the requirements for CJD diagnosis according to WHO diagnostic criteria. (csnn.eu)
- Skupinu proteinu 14-3-3 tvoří sedm izotypových signálních molekul, jejichž přesná funkce však doposud není známa zcela jednoznačně. (csnn.eu)
- Nález ß-podjednotky proteinu 14-3-3 v cerebrospinálním likvoru je jedním z hlavních paraklinických diagnostických markerů sporadické formy Creutzfeldtovy-Jakobovy nemoci (sCJN). (csnn.eu)
- Průkaz proteinu 14-3-3 byl prováděn standardizovanou metodou western blot s chemiluminiscenční detekcí ß-podjednotky ve vzorku likvoru pacientů s podezřením na CJN. (csnn.eu)
- Z celkového počtu 280 likvorových vzorků bylo 57 pozitivních v průkazu proteinu 14-3-3 a 20 nálezů bylo pozitivních hraničně/slabě. (csnn.eu)
- Ve 25 případech pozitivity proteinu 14-3-3 v likvoru a u šesti hraničních nálezů autopsie potvrdila prionové onemocnění typu CJN. (csnn.eu)
- V 18 případech později hodnocených jako prokázaná CJN bylo vyšetření proteinu 14-3-3 negativní. (csnn.eu)
- Naše výsledky odpovídají literárním údajům o rozporuplnosti stanovování ß-podjednotky proteinu 14-3-3 v likvoru. (csnn.eu)
- V porovnání s recentními publikacemi je senzitivita a specificita průkazu proteinu 14-3-3 v likvoru pro diagnózu CJN v našem souboru významně nižší, což může být ovlivněno menším počtem vyšetřených pacientů. (csnn.eu)
- Průkaz proteinu 14-3-3 v likvoru je dle doporučení WHO jednou z podmínek pro klinickou diagnózu CJN. (csnn.eu)
- In comparison to recent publications both sensitivity and specificity of 14-3-3 protein detection for CJD diagnosis is significantly lower in our patient group. (csnn.eu)
- Our results correspond to previously published data, where a predictive value in ß-subunit of protein 14-3-3 detection in CSF is discussed. (csnn.eu)
- We offer 14-3-3 zeta/delta Peptides and 14-3-3 zeta/delta Proteins for use in common research applications: ELISA, Protein Array, SDS-Page, Western Blot. (novusbio.com)
- Each 14-3-3 zeta/delta Peptide and 14-3-3 zeta/delta Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
- Our 14-3-3 zeta/delta Peptides and 14-3-3 zeta/delta Proteins can be used in a variety of model species: Human. (novusbio.com)
- Choose from our 14-3-3 zeta/delta Peptides and Proteins. (novusbio.com)
- The interacting protein was purified by sequential DEAE and size exclusion chromatography, and subsequent microsequencing revealed that they are the zeta isoform of 14-3-3 proteins. (rti.org)
- 1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. (sinobiological.com)
- 14-3-3 proteins in cell cycle regulation. (nih.gov)
- In addition, 14-3-3 proteins have been implicated in the transcriptional regulation of CDK-inhibitors as they modulate the transcription factors p53, FOXO and MIZ1. (nih.gov)
- Effects of 14-3-3 proteins on cell cycle progression and the regulation of 14-3-3 activity during the cell cycle are reviewed in this chapter. (nih.gov)
- In this review, we examine the factors that may regulate the binding of 14-3-3s to their target proteins, and discuss their possible roles in the regulation of the activity and proteolytic degradation of enzymes involved in primary carbon and nitrogen metabolism. (nih.gov)
- This protein family binds to a variety of proteins in a phosphorylation-dependent manner and participates in the regulation of cell proliferation, differentiation and function. (springer.com)
- A postdoctoral position is available to investigate the role of 14-3-3 proteins in the regulation of the plant plasma membrane H+-ATPase and nitrate reductase, an enzyme which is located in the cytosol. (bio.net)
- Reversible protein phosphorylation events are known to play a crucial role in the regulation of stomatal movements. (frontiersin.org)
- However, in some cases, phosphorylation alone is not sufficient to achieve complete protein regulation, but is necessary to mediate the binding of interactors that modulate protein function. (frontiersin.org)
- In this review, we describe the current knowledge about 14-3-3 roles in the regulation of their binding partners in guard cells: receptors, ion pumps, channels, protein kinases, and some of their substrates. (frontiersin.org)
- Regulation of these targets by 14-3-3 proteins is discussed and related to their function in guard cells during stomatal movements in response to abiotic or biotic stresses. (frontiersin.org)
- Reversible protein phosphorylation is recognized as one of the most important post-translational modifications in eukaryotes, playing major roles in the regulation of cellular processes ( Cohen, 2002 ). (frontiersin.org)
- Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. (abcam.com)
- Phosphorylation-dependent binding between tuberin and members of the 14-3-3 protein family indicates how the tuberin-hamartin complex may interact with upstream and downstream effectors, and suggests how phosphorylation-dependent regulation of the complex may be controlled. (biochemsoctrans.org)
- 14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. (acris-antibodies.com)
- The binding of 14-3-3 gamma to glial fibrillary acidic protein is important in the regulation of glial filaments. (acris-antibodies.com)
- Spatial regulation of motor and nonmotor microtubule-associated proteins is crucial to overcoming these challenges. (rupress.org)
- In this study, we tried to investigate which mechanism plays more important role in the 14‐3‐3 σ regulation, how the change of the 14‐3‐3 σ expression affect the biology of human breast cancer, and hence to extend our understanding of the role of the 14‐3‐3 σ in human breast cancer. (aacrjournals.org)
- To investigate the role of methylation in regulation of 14‐3‐3 σ, we examined the methylation status of 14‐3‐3 σ promoter in human breast carcinoma tissue by methylation‐specific polymerase chain reaction. (aacrjournals.org)
- These results suggest that 14‐3‐3 σ may be regulated by both mechanisms i.e. ubiquitin‐mediated proteolysis by Efp and down‐regulation by hypermethylation in human breast cancers. (aacrjournals.org)
- therefore, 14-3-3ε serves functions in the regulation of the cell cycle ( 3 , 4 ) and apoptosis ( 5 - 8 ). (spandidos-publications.com)
- Proper CAR formation and constriction is heavily reliant on the temporal regulation, phosphorylation, and localization of key proteins. (gvsu.edu)
- These proteins associate with many intracellular proteins involved in a variety of cellular processes including regulation of the cell cycle, metabolism and protein trafficking. (biomedcentral.com)
- There is also interest in understanding the role of 14-3-3 proteins in the regulation of oogenesis and the cell cycle during oocyte maturation and in early development. (biomedcentral.com)
- Our findings provide new evidence that 14-3-3 proteins play important roles in neurogenesis and neuronal migration via the regulation of distinct signaling cascades. (jneurosci.org)
- The R7 group of RGS proteins (R7 RGS) has received special attention due to their pivotal roles in the regulation of a range of crucial neuronal processes such as vision, motor control, reward behavior, and nociception in mammals. (springer.com)
- 2003). Insights into G protein structure, function, and regulation. (springer.com)
- The 14-3-3 protein is a family of acidic, dimeric proteins distributed widely among eukaryotic cells. (springer.com)
- 14-3-3 proteins are a family of conserved regulatory molecules that are expressed in all eukaryotic cells. (wikipedia.org)
- Sequence information, as well as subsequent immunological analysis, proved that both polypeptides are members of the eukaryotic 14-3-3 family, which comprises structurally conserved regulatory proteins of widespread occurrence and a wide range of functions. (nih.gov)
- The controlled degradation of proteins by the ubiquitin-26S proteasome system is an important regulatory mechanism in eukaryotic cells. (plantcell.org)
- 14-3-3 sigma (also called stratifin or SFN), a tumor suppressor, is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. (proteinlounge.com)
- Collinge, David 2004-10-13 00:00:00 14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. (deepdyve.com)
- The ubiquitous eukaryotic 14-3-3 proteins coordinate multiple cellular processes due to their well-known regulatory function, which is based on specific recognition of phosphorylated motifs in their partners. (ovid.com)
- 14-3-3 cruciform-binding proteins as regulators of eukaryotic DNA replication. (semanticscholar.org)
- The seven members of the human 14-3-3 protein family regulate a diverse range of cell signaling pathways by formation of protein-protein complexes with signaling proteins that contain phosphorylated Ser/Thr residues within specific sequence motifs. (pnas.org)
- 14-3-3s are involved in numerous processes in the cell and they typically bind to phosphorylated motifs in other proteins and regulate their activities. (dissertations.se)
- Substrate adaptors, such as the ETO1/EOLs, regulate the accessibility of substrate proteins to the CUL3 ligase for ubiquitination, and their availability is important for the ubiquitination and, thus, stability of the cognate substrates. (plantcell.org)
- 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. (acris-antibodies.com)
- Plant 14-3-3 proteins are phosphoserine-binding proteins that regulate a wide array of targets via direct protein-protein interactions. (ovid.com)
- Various factors affecting the oligomeric status of 14-3-3 proteins can thus regulate the balance between regulatory phosphomotif binding and genuine chaperone-like activity. (ovid.com)
- The ubiquitous and mighty 14-3-3 proteins regulate a plethora of cellular processes via coordinated binding to hundreds of specifically phosphorylated partners. (ovid.com)
- In conclusion, 14-3-3 proteins promote the cell-surface transport of correctly assembled complexes but do not regulate the activity of KATP channels at the cell surface. (ox.ac.uk)
- As heterotrimeric complexes, R7 RGS proteins not only associate with and regulate a number of G protein signaling pathway components, but have also been found to form complexes with proteins that are not traditionally associated with G protein signaling. (springer.com)
- In the cytoplasm, the encoded protein may function to regulate apoptosis. (wikipedia.org)
- abstract = "The filamentous fungus Trichoderma reesei is a biotechnologically important organism having an excellent capability to secrete hydrolytic enzymes e.g. cellulases.Allthough the protein production technology is well established, the protein secretion apparatus of T. reesei is still insufficiently known. (vtt.fi)
- citation needed] 14-3-3 proteins play an isoform-specific role in class switch recombination. (wikipedia.org)
- Specifically, the structures of the beta isoform in its apo and peptide bound forms showed that its binding site can exhibit structural flexibility to facilitate binding of its protein and peptide partners. (pnas.org)
- 14-3-3 isoform(s) constituting the fusicoccin receptor are distinguishable from other cellular 14-3-3 proteins by their tight association with the plasma membrane. (nih.gov)
- In addition to the precursor protein, it contains 14-3-3 (probably as a dimer) and a heat shock protein Hsp70 isoform. (plantcell.org)
- The first set of 17 SF -samples from patients with inflamed joints were analyzed for 14-3-3 eta isoform by Western blot. (jrheum.org)
- TCR engagement activates the protein tyrosine kinases Lck, Fyn, and ζ chain-associated protein of 70 kD that initiate the signaling cascade and contribute to the assembly of a "signalosome," a multiprotein complex including various enzymes, their substrates, and scaffold/adaptor proteins ( 4 ). (rupress.org)
- The name for the protein family is tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein family (YWHA). (biomedcentral.com)
- Tyrosine hydroxylase or tyrosine 3-monooxygenase is the enzyme responsible for catalyzing the conversion of the amino acid L -tyrosine to L -3,4-dihydroxyphenylalanine ( L -DOPA). (wikipedia.org)
- Tyrosine hydroxylase catalyzes the reaction in which L -tyrosine is hydroxylated in the meta position to obtain L -3,4-dihydroxyphenylalanine ( L -DOPA). (wikipedia.org)
- Tryptophan is a poor substrate for tyrosine hydroxylase, however it can hydroxylate L -phenylalanine to form L -tyrosine and small amounts of 3-hydroxyphenylalanine. (wikipedia.org)
- We found that phosphodocking 14-3-3 proteins stabilize spindle bipolarity in Drosophila melanogaster oocytes. (rupress.org)
- The 14-3-3s form a family of highly conserved proteins found in all eukaryotes that bind to phosphoserine/phosphothreonine-containing motifs. (frontiersin.org)
- This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. (sinobiological.com)
- Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. (sinobiological.com)
- Current results show that a 14-3-3 dimer is simultaneously occupied by two entire binding motifs of the H+-pump - each consisting of two nonclassical sites - in an antiparallel fashion and an unusual conformation. (uni-tuebingen.de)
- It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms interactions with 14-3-3 proteins. (tudelft.nl)
- These clock-driven cycles of phosphorylation / dephosphorylation may affect interactions with 14-3-3 proteins. (plantae.org)
- In most cases, the association with 14-3-3 proteins requires a specific phosphorylation of the protein ligand and mediates cell cycle arrest. (nih.gov)
- Plant 14-3-3 proteins interact specifically with wheat germ lysate-synthesized chloroplast precursor proteins and require an intact phosphorylation motif within the transit sequence. (plantcell.org)
- Roles for plant 14-3-3 proteins in signalling and development. (lancs.ac.uk)
- Many biological roles for plant 14-3-3 proteins have been suggested in recent months. (lancs.ac.uk)
- 14-3-3 proteins are highly conserved chaperone-like proteins that play important roles in many cellular processes. (genetics.org)
- 14-3-3 proteins play particularly important roles in coordinating progression of cells through the cell cycle, regulating their response to DNA damage, and influencing life-death decisions following internal injury or external cytokine-mediated cues (Ref.1 and 2). (proteinlounge.com)
- Here, we describe two different approaches that identify roles for 14-3-3 proteins in signalling and plant development. (lancs.ac.uk)
- kinesin-14), which has well-documented roles in stabilizing a bipolar spindle in oocytes. (rupress.org)
- Drosophila therefore provides an advantage in defining in vivo roles of 14-3-3. (rupress.org)
- Possible roles for the H+-ATPase and 14-3-3 proteins in responses to pathogens are discussed. (lancs.ac.uk)
- This review summarizes our current understanding of the biology of the R7 RGS complexes including their structure/functional organization, protein-protein interactions, and physiological roles. (springer.com)
- 14-3-3 proteins play a key role in coordinating multiple signalling pathways (see Aitken 1996, Trends Cell Biol. (bio.net)
- These genetic interactions suggest that budding-yeast 14-3-3 proteins are multifunctional and may play a role in both vesicular transport and Ras signaling pathways. (pnas.org)
- Calorie restriction (CR) and conserved nutrient-sensing target of rapamycin (TOR) and protein kinase A (PKA) pathways have also been suggested to extend life span by increasing stress response, which protects cells from age-dependent accumulation of oxidative damages. (genetics.org)
- 4. Nagy, G., Oostenbrink, C., and Hritz, J. Exploring the binding pathways of the 14-3-3ζ protein: Structural and free-energy profiles revealed by Hamiltonian replica exchange molecular dynamics with distancefield distance restraints. (findaphd.com)
- G protein-coupled receptor signaling pathways mediate the transmission of signals from the extracellular environment to the generation of cellular responses, a process that is critically important for neurons and neurotransmitter action. (springer.com)
- Indeed, expression of 14-3-3 inhibitor peptides in cells is sufficient to induce apoptosis. (portlandpress.com)
- Interestingly, these 14-3-3 antagonist peptides can sensitize cells for effective killing by anticancer agents such as cisplatin. (portlandpress.com)
- Phosphorylation-dependent and -independent binding have been shown to be targeted to the same site of the 14-3-3 proteins ( 18 ). (pnas.org)
- These studies demonstrate a phosphorylation-dependent translocation of PIF7 when plants are in shade and a novel mechanism involving 14-3-3 proteins, mediated by the retention of PIF7 in the cytoplasm that suppresses the shade response. (elifesciences.org)
- Members of the 14-3-3 family are key proteins in a number of intracellular events, particularly those involving phosphorylation-dependent switching. (biomedcentral.com)
- Both of these designations are currently used for Arabidopsis 14-3-3s in the literature. (frontiersin.org)
- Secondly, we have identified the Arabidopsis ethylene signalling protein, CTR1, as a 14-3-3-interacting protein. (lancs.ac.uk)
- Here, we demonstrate a shade induction of the nuclear localization of dephosphorylated PIF7 and a role for the 14-3-3 proteins in the cytoplasmic retention of PIF7 in Arabidopsis . (elifesciences.org)
- Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. (wikipedia.org)
- However, diagnostic biochemical parameters including 14-3-3 protein, S100, neuron-specific enorase in cerebrospinal fluid (CSF) have been used as diagnostic markers, elevated titers of these markers can also be observed in CSF in other neurodegenerative diseases. (springer.com)
- 1996). The 14-3-3 brain protein in cerebrospinal fluid as a marker for transmissible spongiform encephalopathies. (springer.com)
- 1998). Detection of 14-3-3 protein in the cerebrospinal fluid supports the diagnosis of Creutzfeldt-Jakob disease. (springer.com)
- At present, the testing of 14-3-3 protein in cerebrospinal fluid (CSF) is a standard biomarker test in suspected sporadic Creutzfeldt-Jakob disease (sCJD) diagnosis. (cdc.gov)
- Detection of the ß-subunit of the 14-3-3 protein in cerebrospinal fluid is one of the most important paraclinical markers in sporadic Creutzfeldt-Jakob disease (sCJD) diagnostic procedure. (csnn.eu)
- Protein 14-3-3 detection was performed by a standardized method using western blot with chemoluminiscent detection of ß-subunit in cerebrospinal fluid samples from patients with clinical suspection of CJD. (csnn.eu)
- 57 cerebrospinal fluid samples were 14-3-3 - positive and 20 were weakly positive from all 280 samples in total. (csnn.eu)
- Nevertheless, a positive finding of protein 14-3-3 in the cerebrospinal fluid is one of the requirements for CJD diagnosis according to WHO diagnostic criteria. (csnn.eu)
- Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. (tudelft.nl)
- In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. (wikipedia.org)
- In the lab you will be part of a group of 3 postdocs working on 14-3-3 proteins in barley and Petunia. (bio.net)
- Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. (tudelft.nl)
- First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. (tudelft.nl)
- Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with the powdery mildew fungus. (deepdyve.com)
- Subcellular localization of N protein. (asm.org)
- The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. (wikipedia.org)
- Western blot analysis of 14-3-3 gamma Antibody (Cat. (acris-antibodies.com)
- Western blot analysis of extracts from K562 cells, treated with insulin 0.01U/ml 15', using 14-3-3 gamma Antibody. (acris-antibodies.com)
- 14-3-3ζ Protein is suitable for use in Western Blot. (tebu-bio.com)
- In addition, western blot analysis validated that the expression level of 14‑3‑3ε was downregulated during curcumin‑induced apoptosis of MG‑63 cells compared with that in control cells. (spandidos-publications.com)
- We analyzed CSF samples derived from 100 patients with various neurodegenerative disorders by Western blot of 14-3-3 protein, quantification of total tau (t-tau) protein, and phosphorylated tau (p-tau) protein. (springer.com)
- The main result of our study was the validation of a commercially available 14-3-3 ELISA next to the commonly used Western blot method as a high-throughput screening test. (cdc.gov)
- Průkaz proteinu 14-3-3 byl prováděn standardizovanou metodou western blot s chemiluminiscenční detekcí ß-podjednotky ve vzorku likvoru pacientů s podezřením na CJN. (csnn.eu)
- An atypical form of bovine spongiform encephalopathy has been identified in cattle in Europe, North America and Japan and was designed as L-type BSE (L-BSE) due to the lower apparent molecular mass of the unglycosylated, protease-resistant prion protein (PrPres) detected by western blot compared with classical BSE. (blogspot.com)
- Biochem Soc Trans (2002) 30 (3): A64. (portlandpress.com)
- 2002). Tau protein and 14-3-3 protein in the differential diagnosis of Creutzfeldt-Jakob disease. (springer.com)
- Type-2 ACS proteins have only a putative calcium-dependent protein kinase target site, and type-3 ACS proteins have a short C-terminal domain with no recognized phosphorylation sites ( Chae and Kieber, 2005 ). (plantcell.org)
- 14‐3‐3 sigma (σ) is a negative regulator of the cell cycle and contributes to G2/M arrest. (aacrjournals.org)
- The Efp‐positive cases were more frequently 14‐3‐3 sigma‐negative. (aacrjournals.org)
- Amino acids M1 - S248 (end) of human 14-3-3 sigma. (dundee.ac.uk)
- 14-3-3 proteins activate the auto-inhibited plasma membrane P-type H+ ATPases. (wikipedia.org)
- The P-type plasma membrane H+-ATPases form a group of proteins only found in plants and fungi. (dissertations.se)
- For instance, SLP-76 detaches from plasma membrane-proximal protein complexes a few minutes after TCR stimulation and translocates to a perinuclear compartment ( 12 ). (rupress.org)
- Overlay assays show a fungus-induced increase in binding of digoxygenin-labelled 14-3-3 protein to several proteins including a 100 kDa membrane protein, probably the plasma membrane H+-ATPase. (deepdyve.com)
- We propose that 14-3-3 proteins are involved in an epidermis-specific response to the powdery mildew fungus, possibly via an activation of the plasma membrane H+-ATPase. (deepdyve.com)
- Fusicoccin (FC) is a fungal toxin that activates the plant plasma membrane H+-ATPase by binding with 14-3-3 proteins, causing membrane hyperpolarization. (lancs.ac.uk)
- In the fission yeast S. pombe, Mid1 is an important dimeric CAR scaffolding protein that connects the contractile apparatus to the plasma membrane at the right place and time during cytokinesis. (gvsu.edu)
- TAZ may link events at the plasma membrane and cytoskeleton to nuclear transcription in a manner that can be regulated by 14-3-3. (elsevier.com)
- Co-immunoprecipitation studies, as well as experiments using confocal microscopy, revealed that dopamine favored the association of 14-3-3 protein with the basolateral plasma membrane and its co-localization with the Na(+),K(+)-ATPase alpha-subunit. (mysciencework.com)
- Attenuation of the alpha2AR-14-3-3 interactions in the presence of a phosphorylated Raf-1 peptide corresponding to its 14-3-3 interacting domain (residues 251-266), but not by its non-phosphorylated counterpart, provides evidence for the functional specificity of these interactions and suggests one potential interface for the alpha2AR and 14-3-3 interactions. (rti.org)
- In comparison to recent publications both sensitivity and specificity of 14-3-3 protein detection for CJD diagnosis is significantly lower in our patient group. (csnn.eu)
- Because of its relatively low specificity it is important to consider the relevance of 14-3-3 positive test in each particular clinical case. (csnn.eu)
- Assays for CSF 14-3-3 protein are probably moderately accurate in diagnosing sCJD: sensitivity 92% (95% confidence interval [CI] 89.8-93.6), specificity 80% (95% CI 77.4-83.0), likelihood ratio of 4.7, and negative likelihood ratio of 0.10. (blogspot.com)
- citation needed] Phosphorylation of Cdc25C by CDS1 and CHEK1 creates a binding site for the 14-3-3 family of phosphoserine binding proteins. (wikipedia.org)
- Moreover, mass spectrometric analyses detected specific phosphorylation of the canonical 14-3-3 binding site (RSXpSXP, where pS denotes phosphoserine) located in the amino-terminal region of HopQ1. (plantphysiol.org)
- C) pCDNA3.1 (lane 2) or pCDNA3.1 N (lanes 1 and 3)-transfected and 35 S Promix-labeled cells were immunoprecipitated with anti-Myc antibody and Western blotted with phosphoserine antibody. (asm.org)
- 19,20 Notably, 14-3-3 proteins were the first molecules identified as discrete binding partners of phosphoserine/threonine (pSer/Thr) residues in proteins, recognizing the sequence RSXpSXP or RXXXpSXP. (ahajournals.org)
- 14-3-3 proteins were the first molecules to be identified as discrete phosphoserine/ threonine binding modules and are highly conserved in both the animal and plant kingdoms. (uni-tuebingen.de)
- The antiapoptotic PPARδ might affect different proteins in endothelial cells, with 14-3-3 proteins being key candidates because of their ability to promote cell survival. (ahajournals.org)
- Because 14-3-3 proteins and 14-3-3ε are antiapoptotic and antiinflammatory molecules in endothelial cells, they may play an important role in atherothrombosis. (ahajournals.org)
- In an attempt to identify the most prominent proteins expressed by this bacterium, especially those encoded by the major antigenic protein 1 (map1) multigene family , a proteome map of ER cultivated in endothelial cells was constructed by using two dimensional gel electrophoresis combined with mass spectrometry . (bvsalud.org)
- Overall , the results presented herein not only reveal the first partial proteome map of ER but provide new insights on the expression ER MAP1- family proteins in host endothelial cells . (bvsalud.org)
- The 14-3-3 protein family contains seven members that are highly conserved and ubiquitously expressed ( 1 ). (pnas.org)
- 14-3-3 proteins are highly conserved and ubiquitously expressed. (ibl-america.com)
- Type-1 ACS proteins contain target sites for both mitogen-activated protein kinase and calcium-dependent protein kinase phosphorylation. (plantcell.org)
- In a prospective study of 110 patients done in The Netherlands (see reference below), it was found that the 14-3-3 assay was 97% sensitive and 87% specific. (blogspot.com)
- Therefore, we concluded that the assay of t-tau protein may be useful as 1st screening and the ratio of p-tau protein/t-tau protein would be useful as 2nd screening to discriminate CJD from other neurodegenerative diseases. (springer.com)
- In order to further improve the detection sensitivity of 14-3-3, a sandwich time-resolved fluoroimmunoassay (TRFIA) was developed using a pair of monoclonal antibodies, and it could be shown that TRFIA has a higher sensitivity in detecting 14-3-3 antigen of S. japonicum than an enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
- Both alpha-SNAP and 14-3-3 proteins stimulated in a priming but not in a triggering assay. (rupress.org)
- Metabolic enzymes as targets for 14-3-3 proteins. (nih.gov)
- translocation within the cell and docking to the respective targets proteins) will be studied by means of Green Fuorescent Protein (GFP) constructs. (bio.net)
- Moreover, swapping the amphipathic C-terminus with a fragment from Vibrio Vis toxin creates a 14-3-3 independent toxin that ADP-ribosylates known ExoS targets. (diva-portal.org)
- Blackledge, M. and Žídek, L.: Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. (findaphd.com)
- Importantly, these activities are usually independent of substrate phosphorylation and therefore should be considered as distinct, 'moonlighting' functions of 14-3-3 proteins that mimic and complement the functions of dedicated molecular chaperones. (ovid.com)
- Recent evidence that 14-3-3 proteins interfere with protein aggregate formation suggests they can also display a distinct, usually phosphorylation-independent, chaperone-like activity, which we review here as their 'moonlighting' function, which is of particular relevance in the context of neurodegenerative disorders. (ovid.com)
- Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis. (rupress.org)
- The effects of alpha-SNAP and 14-3-3 proteins were likely to be due to distinct mechanisms of action since they differed in Ca(2+)-dependency, time course and extent of stimulation and their effects were additive. (rupress.org)
- The data show that these three proteins have distinct stage-specific actions on exocytosis and indicate that alpha-SNAP acts in an early MgATP-requiring stage and not in the late Ca(2+)-triggered steps immediately prior to membrane fusion as previously suggested. (rupress.org)
- Urinary protein SDSPAGE analysis showed eight distinct bands in the urine of heartworm-infected dogs . (bvsalud.org)
- Applying temperature-induced Triton X-114 phase separation experiments, they, as well as the target enzyme of fusicoccin action, the H(+)-ATPase, partitioned into the phospholipid-rich fraction which contains the most hydrophobic proteins. (nih.gov)
- Fusicoccin, 14-3-3 proteins and defense responses in tomato plants. (lancs.ac.uk)
- Fusicoccin, 14-3-3 proteins and defense respons. (lancs.ac.uk)
- This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. (mybiosource.com)
- 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 1% identical to the mouse ortholog. (sinobiological.com)
- The p53 tumour-suppressor protein is a sequence-specific DNA-binding transcription factor that induces cell cycle arrest or apoptosis in response to genotoxic stress. (nih.gov)
- Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
- Several sequence analyses of these proteins have shown 4 regions with a high level of amino acid variability in loops I, V, VI, and VII (variable regions [VRs] 1-4) ( 2 ). (cdc.gov)
- The most likely origin of the porB2/3 hybrid (4, Eb, 2ab, Cc) is the acquisition of DNA that encodes a VR1-4 sequence by a meningococcus with a porB2-99 allelic variant. (cdc.gov)
- ABC proteins transport various molecules across extra- and intracellular membranes. (genecards.org)
- The 14-3-3 protein family are highly conserved soluble acidic proteins ( 1 ). (spandidos-publications.com)
- The 14-3-3 (YWHA) proteins are a highly conserved, ubiquitously expressed family of proteins. (biomedcentral.com)
- 14-3-3 binding may lead to cytoplasmic sequestration of the protein ligand but may also have other functional consequences. (nih.gov)
- In response to the spatial cue provided by Aurora B, 14-3-3 promotes Ncd binding specifically to spindle microtubules by preventing it from binding other microtubules in the large cytoplasmic volume of oocytes. (rupress.org)
- The arrow shows cytoplasmic localization of the N protein. (asm.org)
- The membrane (lane2) and cytoplasmic (lane 3) fractions were immunoprecipitated with anti-Myc antibody. (asm.org)
- Cytoplasmic (lane 2) and nuclear (lane 3) fractions were immunoprecipitated using anti-Myc antibody, resolved by 12% SDS-PAGE, and the protein bands were detected by fluorography. (asm.org)
- Subsequently, the altered expression and localization of 14‑3‑3ε and its co‑localization with other apoptosis‑associated proteins during apoptosis was investigated. (spandidos-publications.com)
- The results of the present study revealed that 14‑3‑3ε is an NMP in MG‑63 cells, and its altered expression and co‑localization with apoptosis‑associated proteins indicated an important function of 14‑3‑3ε in apoptosis of MG‑63 cells. (spandidos-publications.com)
- Grawe, Anissa P., "14-3-3 Protein Rad24 and its Effect on Mid1 Localization and Contractile Acto- Myosin Ring Assembly During Cytokinesis in S. pombe" (2018). (gvsu.edu)
- 2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). (sinobiological.com)
- Coordination of a client protein within the amphipathic groove of a 14-3-3 dimer can have a range of contextdependent effects such as conformational change, re-localization and bridging of two molecules. (uni-tuebingen.de)
- PorB outer membrane proteins are transmembrane proteins with 8 predicted surface-exposed loops (I-VIII), which vary in length and in amino acid sequences. (cdc.gov)
- Four proteins in this group, RGS6, RGS7, RGS9, and RGS11, share a common molecular organization of three modules: (i) the catalytic RGS domain, (ii) a GGL domain that recruits Gβ 5 , an outlying member of the G protein beta subunit family, and (iii) a DEP/DHEX domain that mediates interactions with the membrane anchor proteins R7BP and R9AP. (springer.com)
- Candidates should have demonstrated experience and publications in biochemistry/protein chemistry and molecular biology. (bio.net)
- In this work, we found that the apo-14-3-3σ is in an open state compared with the phosphopeptide bound 14-3-3σ complex which is in a more closed state based on our 80 ns molecular dynamics (MD) simulations. (mdpi.com)
- Hu G, Li H, Liu J-Y, Wang J. Insight into Conformational Change for 14-3-3σ Protein by Molecular Dynamics Simulation. (mdpi.com)
- Molecular binding mode involving 14-3-3 will be elucidated by NMR. (findaphd.com)
- Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 141 (3 Supp). (lancs.ac.uk)
- Understanding the latter mode of 14-3-3 functioning is fundamental to defining the underlying molecular mechanisms for a range of human disorders. (ovid.com)
- In addition to protein crystallization and structure determination, an array of biochemical, molecular and cell biological approaches is applied. (uni-tuebingen.de)
- In this study, we describe a molecular mechanism linking phosphorylation of Na(+),K(+)-ATPase alpha-subunit to binding and activation of phosphoinositide 3-kinase. (mysciencework.com)
- Now we report that one of the suppressor loci, BMH2/SCD3, encodes a protein of the 14-3-3 family. (pnas.org)
- Among the phosphopeptide-binding proteins, the 14-3-3 proteins are the best characterized in plants. (frontiersin.org)
- Effector-triggered immunity represents a second layer of defense, whereby plants have evolved mechanisms that rely upon Resistance (R) proteins to sense and respond to cognate TTSS effectors. (plantphysiol.org)
- Thus, the expression of a specific bacterial effector can either sustain disease in susceptible plants or render the pathogen avirulent in resistant plants that express the corresponding R protein. (plantphysiol.org)
- This indicates an unrecognized selectivity of 14-3-3 proteins for precursors from mitochondria and plastids in plants in comparison to fungi and animals. (plantcell.org)
- We conclude that the 14-3-3-Hsp70-precursor protein complex is a bona fide intermediate in the in vivo protein import pathway in plants. (plantcell.org)
- We find that mutation of a 14-3-3 binding site in CTR1 blocks its activity when introduced into transgenic plants, resulting in constitutive ethylene signalling and aberrant plant development. (lancs.ac.uk)
- This highly conserved protein family is found in both plants and mammals. (mybiosource.com)
- Research in my laboratory aims to understand the biological functions of 14-3-3 proteins in higher plants. (uni-tuebingen.de)
- 90% of their protein constituents from the cytosol in a post-translational event. (plantcell.org)
- In addition, the cells must retard folding of the organellar proteins before these polypeptides reach their final destination to avoid the biological function of the proteins in the wrong compartment, namely, the cytosol. (plantcell.org)
- Small volumes of 14-3-3 alpha/beta recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
- Small volumes of YWHAE recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
- In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. (sinobiological.com)
- Bulk packages of recombinant proteins are provided as frozen liquid. (sinobiological.com)
- By contrast, 14-3-3 proteins functionally antagonized the Arg-based signal present in SUR1. (ox.ac.uk)
- To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. (rti.org)
- C) Van der Waals surface representation of one amphipathic 14-3-3 binding cleft showing the close contact of both ligands. (uni-tuebingen.de)
- 14-3-3 proteins promote survival in part by antagonizing the activity of associated proapoptotic proteins, including Bad and apoptosis signal-regulating kinase 1 (ASK1). (portlandpress.com)
- By binding in the 14-3-3 phosphopeptide binding groove, an amphipathic C-terminal helix of ExoS and ExoT has been thought to be crucial for their activation. (diva-portal.org)