Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.
A rare transmissible encephalopathy most prevalent between the ages of 50 and 70 years. Affected individuals may present with sleep disturbances, personality changes, ATAXIA; APHASIA, visual loss, weakness, muscle atrophy, MYOCLONUS, progressive dementia, and death within one year of disease onset. A familial form exhibiting autosomal dominant inheritance and a new variant CJD (potentially associated with ENCEPHALOPATHY, BOVINE SPONGIFORM) have been described. Pathological features include prominent cerebellar and cerebral cortical spongiform degeneration and the presence of PRIONS. (From N Engl J Med, 1998 Dec 31;339(27))
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.

An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast. (1/1483)

The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well.  (+info)

Cell adhesion regulates the interaction between the docking protein p130(Cas) and the 14-3-3 proteins. (2/1483)

Integrin ligand binding induces a signaling complex formation via the direct association of the docking protein p130(Cas) (Cas) with diverse molecules. We report here that the 14-3-3zeta protein interacts with Cas in the yeast two-hybrid assay. We also found that the two proteins associate in mammalian cells and that this interaction takes place in a phosphoserine-dependent manner, because treatment of Cas with a serine phosphatase greatly reduced its ability to bind 14-3-3zeta. Furthermore, the Cas-14-3-3zeta interaction was found to be regulated by integrin-mediated cell adhesion. Thus, when cells are detached from the extracellular matrix, the binding of Cas to 14-3-3zeta is greatly diminished, whereas replating the cells onto fibronectin rapidly induces the association. Consistent with these results, we found that the subcellular localization of Cas and 14-3-3 is also regulated by integrin ligand binding and that the two proteins display a significant co-localization during cell attachment to the extracellular matrix. In conclusion, our results demonstrate that 14-3-3 proteins participate in integrin-activated signaling pathways through their interaction with Cas, which, in turn, may contribute to important biological responses regulated by cell adhesion to the extracellular matrix.  (+info)

Protein kinase C mu is negatively regulated by 14-3-3 signal transduction proteins. (3/1483)

Recent studies have documented direct interaction between 14-3-3 proteins and key molecules in signal transduction pathways like Ras, Cbl, and protein kinases. In T cells, the 14-3-3tau isoform has been shown to associate with protein kinase C theta and to negatively regulate interleukin-2 secretion. Here we present data that 14-3-3tau interacts with protein kinase C mu (PKCmu), a subtype that differs from other PKC members in structure and activation mechanisms. Specific interaction of PKCmu and 14-3-3tau can be shown in the T cell line Jurkat by immunocoprecipitiation and by pulldown assays of either endogenous or overexpressed proteins using PKCmu-specific antibodies and GST-14-3-3 fusion proteins, respectively. Using PKCmu deletion mutants, the 14-3-3tau binding region is mapped within the regulatory C1 domain. Binding of 14-3-3tau to PKCmu is significantly enhanced upon phorbol ester stimulation of PKCmu kinase activity in Jurkat cells and occurs via a Cbl-like serine containing consensus motif. However, 14-3-3tau is not a substrate of PKCmu. In contrast 14-3-3tau strongly down-regulates PKCmu kinase activity in vitro. Moreover, overexpression of 14-3-3tau significantly reduced phorbol ester induced activation of PKCmu kinase activity in intact cells. We therefore conclude that 14-3-3tau is a negative regulator of PKCmu in T cells.  (+info)

Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. (4/1483)

Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/ threonine kinase Akt, which then phosphorylates and inactivates components of the apoptotic machinery, including BAD and Caspase 9. In this study, we demonstrate that Akt also regulates the activity of FKHRL1, a member of the Forkhead family of transcription factors. In the presence of survival factors, Akt phosphorylates FKHRL1, leading to FKHRL1's association with 14-3-3 proteins and FKHRL1's retention in the cytoplasm. Survival factor withdrawal leads to FKHRL1 dephosphorylation, nuclear translocation, and target gene activation. Within the nucleus, FKHRL1 triggers apoptosis most likely by inducing the expression of genes that are critical for cell death, such as the Fas ligand gene.  (+info)

Ca2+-induced apoptosis through calcineurin dephosphorylation of BAD. (5/1483)

The Ca2+-activated protein phosphatase calcineurin induces apoptosis, but the mechanism is unknown. Calcineurin was found to dephosphorylate BAD, a pro-apoptotic member of the Bcl-2 family, thus enhancing BAD heterodimerization with Bcl-xL and promoting apoptosis. The Ca2+-induced dephosphorylation of BAD correlated with its dissociation from 14-3-3 in the cytosol and translocation to mitochondria where Bcl-xL resides. In hippocampal neurons, L-glutamate, an inducer of Ca2+ influx and calcineurin activation, triggered mitochondrial targeting of BAD and apoptosis, which were both suppressible by coexpression of a dominant-inhibitory mutant of calcineurin or pharmacological inhibitors of this phosphatase. Thus, a Ca2+-inducible mechanism for apoptosis induction operates by regulating BAD phosphorylation and localization in cells.  (+info)

Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants. (6/1483)

Fusicoccin (FC) is a fungal toxin that activates the plant plasma membrane H+-ATPase by binding with 14-3-3 proteins, causing membrane hyperpolarization. Here we report on the effect of FC on a gene-for-gene pathogen-resistance response and show that FC application induces the expression of several genes involved in plant responses to pathogens. Ten members of the FC-binding 14-3-3 protein gene family were isolated from tomato (Lycopersicon esculentum) to characterize their role in defense responses. Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. Different specific subsets of 14-3-3 genes were induced after treatment with FC and during a gene-for-gene resistance response. Possible roles for the H+-ATPase and 14-3-3 proteins in responses to pathogens are discussed.  (+info)

Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import. (7/1483)

Cdc2-cyclin B1 in the G2-arrested Xenopus oocyte is held inactive by phosphorylation of Cdc2 at two negative regulatory sites, Thr14 and Tyr15. Upon treatment with progesterone, these sites are dephosphorylated by the dual specificity phosphatase, Cdc25, leading to Cdc2-cyclin B1 activation. Whereas maintenance of the G2 arrest depends upon preventing Cdc25-induced Cdc2 dephosphorylation, the mechanisms responsible for keeping Cdc25 in check in these cells have not yet been described. Here we report that Cdc25 in the G2-arrested oocyte is bound to 14-3-3 proteins and that progesterone treatment abrogates this binding. We demonstrate that Cdc25, apparently statically localized in the cytoplasm, is actually capable of shuttling in and out of the oocyte nucleus. Binding of 14-3-3 protein markedly reduces the nuclear import rate of Cdc25, allowing nuclear export mediated by a nuclear export sequence present in the N-terminus of Cdc25 to predominate. If 14-3-3 binding to Cdc25 is prevented while nuclear export is inhibited, the coordinate nuclear accumulation of Cdc25 and Cdc2-cyclin B1 facilitates their mutual activation, thereby promoting oocyte maturation.  (+info)

The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2-adrenergic receptor subtypes. (8/1483)

The alpha2-adrenergic receptors (alpha2ARs) are localized to and function on the basolateral surface in polarized renal epithelial cells via a mechanism involving the third cytoplasmic loop. To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. A protein doublet of approximately 30 kDa in Madin-Darby canine kidney cells or pig brain cytosol (alpha2B >/= alpha2C>> alpha2A) was identified. The interacting protein was purified by sequential DEAE and size exclusion chromatography, and subsequent microsequencing revealed that they are the zeta isoform of 14-3-3 proteins. [35S]Met-14-3-3zeta binds to all three native alpha2AR subtypes, assessed using a solid phase binding assay (alpha2A>/=alpha2B> alpha2C), and this binding depends on the presence of the 3i loops. Attenuation of the alpha2AR-14-3-3 interactions in the presence of a phosphorylated Raf-1 peptide corresponding to its 14-3-3 interacting domain (residues 251-266), but not by its non-phosphorylated counterpart, provides evidence for the functional specificity of these interactions and suggests one potential interface for the alpha2AR and 14-3-3 interactions. These studies represent the first evidence for G protein-coupled receptor interactions with 14-3-3 proteins and may provide a mechanism for receptor localization and/or coordination of signal transduction.  (+info)

An encyclopedia is a comprehensive reference work containing articles on various topics, usually arranged in alphabetical order. In the context of medicine, a medical encyclopedia is a collection of articles that provide information about a wide range of medical topics, including diseases and conditions, treatments, tests, procedures, and anatomy and physiology. Medical encyclopedias may be published in print or electronic formats and are often used as a starting point for researching medical topics. They can provide reliable and accurate information on medical subjects, making them useful resources for healthcare professionals, students, and patients alike. Some well-known examples of medical encyclopedias include the Merck Manual and the Stedman's Medical Dictionary.

Electrophoresis, starch gel is a type of electrophoretic technique used in laboratory settings for the separation and analysis of large biomolecules such as DNA, RNA, and proteins. In this method, a gel made from cooked starch is used as the supporting matrix for the molecules being separated.

The sample containing the mixture of biomolecules is loaded onto the gel and an electric field is applied, causing the negatively charged molecules to migrate towards the positive electrode. The starch gel acts as a molecular sieve, with smaller molecules moving more quickly through the gel than larger ones. This results in the separation of the mixture into individual components based on their size and charge.

Once the separation is complete, the gel can be stained to visualize the separated bands. Different staining techniques are used depending on the type of biomolecule being analyzed. For example, proteins can be stained with dyes such as Coomassie Brilliant Blue or silver nitrate, while nucleic acids can be stained with dyes such as ethidium bromide.

Starch gel electrophoresis is a relatively simple and inexpensive technique that has been widely used in molecular biology research and diagnostic applications. However, it has largely been replaced by other electrophoretic techniques, such as polyacrylamide gel electrophoresis (PAGE), which offer higher resolution and can be automated for high-throughput analysis.

Creutzfeldt-Jakob syndrome (CJD) is a rare, degenerative, and fatal brain disorder. It is caused by an abnormal form of protein called prion that can cause normal proteins in the brain to fold into abnormal shapes and accumulate, leading to damage and death of brain cells.

The symptoms of CJD usually develop over a period of several months and include rapidly progressing dementia, memory loss, confusion, coordination problems, muscle stiffness, twitching, and shaking. Some people may also experience visual hallucinations, changes in personality, or depression.

There are three main types of CJD: sporadic, inherited, and acquired. Sporadic CJD is the most common form and accounts for about 85% of all cases. It occurs spontaneously with no known cause. Inherited CJD is caused by a genetic mutation that is passed down from parents to their children. Acquired CJD is caused by exposure to contaminated tissue or bodily fluids, such as through a medical procedure or eating contaminated beef (variant CJD).

There is no cure for Creutzfeldt-Jakob syndrome and it is fatal, usually within a year of onset of symptoms. Treatment focuses on managing the symptoms and making the patient as comfortable as possible.

Electrophoresis is a laboratory technique used in the field of molecular biology and chemistry to separate charged particles, such as DNA, RNA, or proteins, based on their size and charge. This technique uses an electric field to drive the movement of these charged particles through a medium, such as gel or liquid.

In electrophoresis, the sample containing the particles to be separated is placed in a matrix, such as a gel or a capillary tube, and an electric current is applied. The particles in the sample have a net charge, either positive or negative, which causes them to move through the matrix towards the oppositely charged electrode.

The rate at which the particles move through the matrix depends on their size and charge. Larger particles move more slowly than smaller ones, and particles with a higher charge-to-mass ratio move faster than those with a lower charge-to-mass ratio. By comparing the distance that each particle travels in the matrix, researchers can identify and quantify the different components of a mixture.

Electrophoresis has many applications in molecular biology and medicine, including DNA sequencing, genetic fingerprinting, protein analysis, and diagnosis of genetic disorders.

DEAE-cellulose chromatography is a method of purification and separation of biological molecules such as proteins, nucleic acids, and enzymes. DEAE stands for diethylaminoethyl, which is a type of charged functional group that is covalently bound to cellulose, creating a matrix with positive charges.

In this method, the mixture of biological molecules is applied to a column packed with DEAE-cellulose. The positively charged DEAE groups attract and bind negatively charged molecules in the mixture, such as nucleic acids and proteins, while allowing uncharged or neutrally charged molecules to pass through.

By adjusting the pH, ionic strength, or concentration of salt in the buffer solution used to elute the bound molecules from the column, it is possible to selectively elute specific molecules based on their charge and binding affinity to the DEAE-cellulose matrix. This makes DEAE-cellulose chromatography a powerful tool for purifying and separating biological molecules with high resolution and efficiency.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

I'm happy to help! However, it seems there might be a bit of confusion here. Starch is not typically considered a medical term, but rather a nutritional and biochemical concept. Here's a brief explanation:

Starch is a complex carbohydrate that serves as the primary form of energy storage in plants. It is made up of long chains of glucose molecules and can be found in various foods such as grains, legumes, fruits, and vegetables. Amylase, an enzyme present in our saliva and digestive system, helps break down starch into simpler sugars during the digestion process so that our bodies can absorb them for energy.

I hope this clarifies any confusion! If you have any other questions or need further information on a medical topic, please don't hesitate to ask.

"Structural basis for protein-protein interactions in the 14-3-3 protein family". Proc. Natl. Acad. Sci. U.S.A. 103 (46): 17237- ... These proteins contain a number of known common modification domains, including regions for divalent cation interaction, ... To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain.[ ... More than 200 signaling proteins have been reported as 14-3-3 ligands. Elevated amounts of 14-3-3 proteins in cerebrospinal ...
2000). "Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins". ... "The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins". Cell. 83 ... The cIAP2 protein contains three BIR domains, a UBA domain, a CARD domain and a RING finger domain. Transcript variants ... The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to ...
Look up tau protein or tau in Wiktionary, the free dictionary. tau+Proteins at the U.S. National Library of Medicine Medical ... The tau proteins were identified in 1975 as heat-stable proteins essential for microtubule assembly, and since then they have ... Tau protein has been shown to interact with: Alpha-synuclein, FYN, Proto-oncogene tyrosine-protein kinase Src S100B, and YWHAZ ... The tau proteins (abbreviated from tubulin associated unit) are a group of six highly soluble protein isoforms produced by ...
Serine/threonine-protein kinase D1 is an enzyme that in humans is encoded by the PRKD1 gene. Members of the protein kinase D ( ... Holmes AM (1996). "In vitro phosphorylation of human immunodeficiency virus type 1 Tat protein by protein kinase C: evidence ... "Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-alpha, and -epsilon and - ... "The pleckstrin homology domain of protein kinase D interacts preferentially with the eta isoform of protein kinase C". J. Biol ...
... , is a protein that in humans is encoded by the MSX1 gene. MSX1 transcripts are not only found in ... MSX1 and LHX2 form a protein complex in the absence of DNA, and that DNA binding by either protein alone can occur at the ... Phenotypes caused by deficiency of MSX1 protein might depend on the localization of mutations and their effect on the protein ... MSX1 has been shown to interact with DLX5, CREB binding protein, Sp1 transcription factor, DLX2, TATA binding protein and Msh ...
The yeast homolog Sec17, pictured above contains fourteen α-helices and has the approximate dimensions of 85 Å × 35 Å × 35 Å ... SNAPs associate with the proteins of the SNARE (SNAP REceptor) complex, a class of type II integral membrane protein, as well ... Some of the first proteins identified as the receptors of SNAPs were syntaxin 1, SNAP-25 (synaptosome associated protein, 25kDa ... SNAPs interact with proteins of the SNARE complex and NSF to play a key role in recycling the components of the fusion complex ...
"Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins". Proteins. 65 (3): 643-655. doi: ... Notably, the protein contains three EF hand motifs that function as binding sites for Ca2+ ions. The protein is a member of the ... In the animal, the protein occurs together with the green fluorescent protein to produce green light by resonant energy ... Because the protein is large, it has a low leakage rate from cells compared to lipophilic dyes such as DiI. It lacks phenomena ...
Protein Science. 14 (3): 812-822. doi:10.1110/ps.04951405. ISSN 0961-8368. PMC 2279270. PMID 15722453. Hulver MW, Berggren JR, ... "Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B ... "Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B ... 3 (4): 271-80. doi:10.2174/1568008033340117. PMID 14683458. Shiwaku K, Hashimoto M, Kitajima K, Nogi A, Anuurad E, Enkhmaa B, ...
Koyata H, Hiraga K (February 1991). "The glycine cleavage system: structure of a cDNA encoding human H-protein, and partial ... Activity is augmented by high-protein diets and starvation. During periods of low carbohydrates, serine is converted into ... Protein Science. 14 (3): 791-8. doi:10.1110/ps.041179105. PMC 2279282. PMID 15689518. "KEGG ENZYME Database Entry". Kyoto ... Figure 3 and Figure 4). The reaction catalyzed by serine dehydratase follows the pattern seen by other PLP-dependent reactions ...
Users can search the database for a particular motion by either protein name or Protein Data Bank ID number. Typically, however ... ISBN 978-0-470-18105-8. Frauenfelder, Hans (10 June 2010). "Chapter 26 on Protein Motions". The Physics of Proteins: An ... users will enter the database via the Protein Data Bank, which often provides a hyperlink to the molmovdb entry for proteins ... Database of protein conformational diversity Gu, Jenny; Bourne, Philip E. (March 2009). Structural Bioinformatics (2nd ed.). ...
... is a protein that in humans is encoded by the SLC25A3 gene. The encoded protein is a ... The encoded protein (PHC) is a multi-pass transmembrane protein located in the mitochondrial inner membrane; it contains six ... "SLC25A3 - Phosphate carrier protein, mitochondrial precursor - Homo sapiens (Human) - SLC25A46 gene & protein". www.uniprot.org ... "SLC25A3 - Phosphate carrier protein, mitochondrial". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). Dolce V, ...
October 2022). "Discovery and Biological Characterization of PRMT5:MEP50 Protein-Protein Interaction Inhibitors". Journal of ... in the binding groove between PRMT5 and peptides derived from the adaptor proteins lead to development of protein-protein ... "A novel WD repeat protein component of the methylosome binds Sm proteins". J. Biol. Chem. 277 (10): 8243-7. doi:10.1074/jbc. ... "Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their ...
The protein belongs to the S5P family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence ... Swiercz R, Person MD, Bedford MT (2005). "Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine ... a protein with a dual subcellular fate, is interacting with human ribosomal protein S2". Biochem. Biophys. Res. Commun. 338 (2 ... Overview of all the structural information available in the PDB for UniProt: P15880 (40S ribosomal protein S2) at the PDBe-KB. ...
Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. WD repeat-containing protein 77 has been shown ... "A novel WD repeat protein component of the methylosome binds Sm proteins". J Biol Chem. 277 (10): 8243-7. doi:10.1074/jbc. ... 2005). "Identification of proteins interacting with the RNAPII FCP1 phosphatase: FCP1 forms a complex with arginine ... Overview of all the structural information available in the PDB for UniProt: Q9BQA1 (Methylosome protein 50) at the PDBe-KB. v ...
The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has ... Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53-63. doi ... "Protein kinase CK2 regulates CDC25B phosphatase activity". Oncogene. 22 (2): 220-32. doi:10.1038/sj.onc.1206107. ISSN 0950-9232 ... 14 (20): 2485-95. doi:10.1038/sj.onc.1201063. PMID 9188863. Booher RN, Holman PS, Fattaey A (1997). "Human Myt1 is a cell cycle ...
Forkhead box O6 is a protein that in humans is encoded by the FOXO6 gene. FoxO6 is expressed in the liver, skeletal muscle, and ... 5 (3): 233-40. doi:10.1111/1753-0407.12027. PMC 3657578. PMID 23324123. Qinyu L, Long C, Zhen-dong D, Min-min S, Wei-ze W, Wei- ... 587 (14): 2105-11. doi:10.1016/j.febslet.2013.05.027. PMID 23714368. S2CID 38064268. v t e (Articles with short description, ... Tzivion G, Dobson M, Ramakrishnan G (November 2011). "FoxO transcription factors; Regulation by AKT and 14-3-3 proteins". ...
This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ... "The kinesin-like motor protein KIF1C occurs in intact cells as a dimer and associates with proteins of the 14-3-3 family". J. ... Han L, Wong D, Dhaka A, Afar D, White M, Xie W, Herschman H, Witte O, Colicelli J (1997). "Protein binding and signaling ... Aoki H, Hayashi J, Moriyama M, Arakawa Y, Hino O (2000). "Hepatitis C Virus Core Protein Interacts with 14-3-3 Protein and ...
M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A is a protein that in humans is encoded by the ... In contrast, some invertebrates harbour two (e.g., the Drosophila proteins String and Twine) or four (e.g., C. elegans Cdc-25.1 ... Dual-specificity protein phosphatases remove phosphate groups from phosphorylated tyrosine and serine / threonine residues. ... Coqueret O, Bérubé G, Nepveu A (1998). "The mammalian Cut homeodomain protein functions as a cell-cycle-dependent ...
Raf kinases A-Raf kinase B-Raf kinase KSR1 protein KSR2 protein GRCm38: Ensembl release 89: ENSMUSG00000000441 - Ensembl, May ... Yuryev A, Wennogle LP (February 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid ... a novel jun N-terminal protein kinase (JNK)-binding protein that functions as a Scaffold factor in the JNK signaling pathway". ... and the enzymes dedicated for removal of these phosphates are the protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A ...
... protein unfolding) or other diagenetic processes. Ancient proteins also tend to be fragmented, damaged and altered. Proteins ... Ancient enamel proteins are useful when aDNA or other proteins do not survive, and they have been analysed to understand ... However, ancient proteins are still one of the most informative biomolecules. Proteins tend to degrade more slowly than DNA, ... There is also the issue of dark proteomes (unknown protein regions that cannot be sequenced); approximately 44-54% of proteins ...
... (SP-A2), also known as Pulmonary surfactant-associated protein A2 (PSP-A2) is a protein that in humans is ... Together with (surfactant protein A1 ) SP-A1, they are the most abundant proteins of pulmonary surfactant. SP-A2 binds to the ... SP-A2 is a protein of 248 amino acids usually found in large oligomeric structures. The mature SP-A2 monomer is a 35kDa protein ... Using specific monoclonal antibodies for Surfactant protein A, the protein can be detected in lung alveolar type II pneumocytes ...
... +protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH) v t e (Articles with short ... or K2P18.1 is a protein that in humans is encoded by the KCNK18 gene. K2P18.1 is a potassium channel containing two pore- ... Czirják G, Vuity D, Enyedi P (2008). "Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation". J. Biol ...
The protein can be a toxin, an apoptotic factor, or a viral protein. Toxins such as diphtheria toxin interfere with cellular ... "Regulation of telomerase activity and anti-apoptotic function by protein-protein interaction and phosphorylation". FEBS Letters ... Viral proteins like viral thymidine kinase can be used for specific targeting of a drug. By introducing a prodrug only ... the introduction of hTERT has an advantage over the use of viral protein for immortalization in that it does not involve the ...
... camp-responsive element-binding protein (CREB) and CREB-binding protein (CBP). It has been proposed that recruitment of ... Mutant atrophin-1 proteins have been found in neuronal intranuclear inclusions (NII) and diffusely accumulated in the neuronal ... Atrophin-1 may be a dispensable or redundant protein as mice bred with a null allele for atrophin-1 produce viable and fertile ... It contains a putative nuclear localization signal in the N-terminus of the protein and a putative nuclear export signal in the ...
This protein family is known for regulating signal transduction pathways primarily through binding phosphoserine proteins, ... is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. The protein encoded by this gene is a member of the ... though it can also bind phosphothreonine proteins and unphosphorylated proteins. By extension, 14-3-3 proteins are involved in ... "New insights into potential functions for the protein 4.1 superfamily of proteins in kidney epithelium". Frontiers in ...
However, the Hd3a protein is found in neither of these places but instead accumulates in the SAM which shows that Hd3a protein ... CO protein promotes transcription of another gene called Flowering Locus T (FT). By this mechanism, CO protein may only reach ... and is then translated into CO protein. However CO protein is stable only in light, so levels stay low throughout short days ... The FT protein resulting from the short period of CO transcription factor activity is then transported via the phloem to the ...
T-cell leukemia homeobox protein 2 is a protein that in humans is encoded by the TLX2 gene. TLX2 has been shown to interact ... Tang SJ, Suen TC, McInnes RR, Buchwald M (September 1998). "Association of the TLX-2 homeodomain and 14-3-3eta signaling ... proteins". The Journal of Biological Chemistry. 273 (39): 25356-63. doi:10.1074/jbc.273.39.25356. PMID 9738002. v t e (Articles ... "Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins". The Journal of Biological Chemistry. 273 (39): 25356-63 ...
The cause was determined to be a histidine-to-tyrosine (amino acid) substitution at position 274 in its neuraminidase protein.[ ... Sialic acid is a negatively charged sugar associated with the protein and lipid portions of lipoproteins.[citation needed] To ... A single hemagglutinin-neuraminidase protein can combine neuraminidase and hemagglutinin functions, such as in mumps virus and ... Neuraminidase also cleaves sialic acid residues from viral proteins, preventing aggregation of viruses.[medical citation needed ...
ISBN 1-59745-189-4. Anson ML, Protein Denaturation and the Properties of Protein Groups, Advances in Protein Chemistry, 2, 361- ... Protein Sci. 4(10), 2138-2148 (1995) Robertson, A.D., Murphy, K.P. Protein structure and the energetics of protein stability, ( ... 1992) "Physical Basis for the Stability of the Folded Conformations of Proteins", in Protein Folding, TE Creighton, ed., W. H. ... "The use of high-pressure nuclear magnetic resonance to study protein folding". In Bai, Yawen; Nussinov, Ruth (eds.). Protein ...
... is a mammalian gene encoding the protein CBL which is an E3 ubiquitin-protein ligase involved in cell signalling and protein ... "The Cbl proteins are binding partners for the Cool/Pix family of p21-activated kinase-binding proteins". FEBS Lett. 550 (1-3): ... "The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src ... "The protein product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the ...
"Structural basis for protein-protein interactions in the 14-3-3 protein family". Proc. Natl. Acad. Sci. U.S.A. 103 (46): 17237- ... These proteins contain a number of known common modification domains, including regions for divalent cation interaction, ... To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain.[ ... More than 200 signaling proteins have been reported as 14-3-3 ligands. Elevated amounts of 14-3-3 proteins in cerebrospinal ...
... evolutionarily conserved proteins expressed abundantly in the nervous system. Although they were discovered more than 30 yr ago ... protein kinases with critical roles in neuronal signaling. These proteins are capable of activating Raf in vitro, and this role ... The interacting proteins, in turn, contain a 14-3-3 binding motif; proteins that interact with 14-3-3 dimers include PKC and ... evolutionarily conserved proteins expressed abundantly in the nervous system. Although they were discovered more than 30 yr ago ...
PorB outer membrane proteins are transmembrane proteins with 8 predicted surface-exposed loops (I-VIII), which vary in length ... proteins are the major proteins found in the outer membrane of Neisseria meningitidis (1); they function as porins, allowing ... Genetics protein I of Neisseria gonorrhoeae: construction of hybrid porins. Proc Natl Acad Sci U S A. 1988;85:6841-5. DOIPubMed ... The porB gene sequence shows VR1-4, which is exclusive of PorB3 protein, and VR2-Eb, VR3-2ab, and VR4-Cc, which are typical of ...
Membrane proteins as 14-3-3 clients in functional regulation and intracellular transport. In: Physiology. 2011 ; Vol. 26, No. 3 ... Membrane proteins as 14-3-3 clients in functional regulation and intracellular transport. / Smith, Andrew J.; Daut, Jürgen; ... Smith AJ, Daut J, Schwappach B. Membrane proteins as 14-3-3 clients in functional regulation and intracellular transport. ... We summarize current progress in identifying kinases that target membrane proteins with 14-3-3 binding sites and discuss the ...
Here, we show 14-3-3 is a reader protein of DNMT1pSer143. In mammalian cells 14-3-3 colocalizes and binds DNMT1pSer143 post-DNA ... Furthermore, 14-3-3 overexpression also resulted in enhanced cell invasion. Therefore, we suggest that 14-3-3 is a crucial ... Binding of 14-3-3 proteins with DNMT1pSer143 resulted in inhibition of DNA methylation activity in vitro. In addition, ... GSE72860: Binding of 14-3-3 reader proteins to phosphorylated DNMT1 facilitates aberrant DNA methylation and gene expression. ...
There is a crucial role of stress response proteins for the survival of the crops in stress conditions. So the study of wheat ... This is the first novel work for analysing the mutational effect on the structure and function of a stress response protein (14 ... The functional diversity of the protein in different species was correlated with mutations, change in secondary structure and ... Functional annotation; Multiple sequence alignment; Mutational effects; Phylogenetic tree; Protein domain analysis; Secondary ...
abstract = "Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MAPKAPK2) mediates multiple p38 MAPK-dependent ... N2 - Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MAPKAPK2) mediates multiple p38 MAPK-dependent ... AB - Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MAPKAPK2) mediates multiple p38 MAPK-dependent ... Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MAPKAPK2) mediates multiple p38 MAPK-dependent inflammatory ...
Serving: 1 ballCalories: 137 kcalCarbohydrates: 14 gProtein: 3 gFat: 8 gSaturated Fat: 2 gMonounsaturated Fat: 3 gTrans Fat: ... Fry the balls in small batches until golden brown, 2 to 3 minutes. Remove with a slotted spoon and drain on paper towels. Serve ...
Jelic et al found a positive correlation between levels of tau protein in the cerebrospinal fluid (CSF) and the EEG alpha/delta ... 14] . QEEG alone showed an accuracy of 77% in the whole group and 69% in those with mild AD; regional CBF alone had an accuracy ... The 14-3-3 protein was detected in the cerebrospinal fluid (CSF). The patient died within 5 months of presentation. [46] ... 18, 19] The QEEG of 31 patients with AD was recorded at the early stage of the disease and after a 3-year follow-up. Patients ...
Jelic et al found a positive correlation between levels of tau protein in the cerebrospinal fluid (CSF) and the EEG alpha/delta ... 14] . QEEG alone showed an accuracy of 77% in the whole group and 69% in those with mild AD; regional CBF alone had an accuracy ... The 14-3-3 protein was detected in the cerebrospinal fluid (CSF). The patient died within 5 months of presentation. [46] ... 18, 19] The QEEG of 31 patients with AD was recorded at the early stage of the disease and after a 3-year follow-up. Patients ...
3×. 1×. Bromoxynil + Pyrasulfotole. 230 g a.i. ha−1 +. 40 g a.i. ha−1. Huskie + 157 g a.i. ha−1 Section 3 (clethodim) +. 2.3 L ... 3×. 1×. Bromoxynil + Pyrasulfotole. 230 g a.i. ha−1 +. 40 g a.i. ha−1. Huskie + 157 g a.i. ha−1 Section 3 (clethodim) +. 2.3 L ... 1.0 ± 3 b. 43.6. 11.2. 29.3 ± 35. 0.0 ± 0 b. WEED-IT_Natural. 36.8. 7.0. 32.9 ± 28. 0.0 ± 0 b. 31.2. 6.6. 52.7 ± 26. 4.9 ± 14 ... 69.5 ± 14 ab. 19.3. 10.5. 19.7 ± 8 a. 71.1 ± 20 a. WeedSeeker_Natural. 5.8. 3.8. 3.8 ± 8 bc. 16.9 ± 25 bc. 5.0. 1.1. 1.0 ± 3 b ...
Proteomics is the large-scaled study of all expressed proteins that provides information about protein abundance and protein ... Fourteen differentially expressed genes were identified. Sarcomatous phenotype alters the EMT process in CCA, resulting in ... Among these proteins, 4 candidate markers actinin 1, actinin 4, protein DJ-1, and cathepsin B were validated by WB and IHC ... Protein microarray has been developed to detect thousands of proteins based on specific antibody detection [17]. Metabolic ...
The core of SidJ adopts a protein kinase fold. Although there is no detectable primary sequence homology to any known protein ... Protein crystallization. Request a detailed protocol Protein crystallization screens were performed on the Crystal Phoenix ... E) In vitro glutamylation of SdeA by SidJ-CaM proteins at a concentration of 50 nM. The proteins in the reactions were ... The digested protein was concentrated and purified further by fast protein liquid chromatography (FPLC) size exclusion ...
Protein automated matches [190669] (6 species). not a true protein. *. Species Human (Homo sapiens) [TaxId:9606] [187771] (5 ... Other proteins in same PDB: d4u19a2, d4u19b2. automated match to d2f6qb_. complexed with cl, edo; mutant. ... Other proteins in same PDB: d4u19a2, d4u19b2. automated match to d2f6qb_. complexed with cl, edo; mutant. ... Other proteins in same PDB: d4u19a2, d4u19b2. automated match to d2f6qb_. complexed with cl, edo; mutant. ...
Protein phosphatase 2A reactivates FOXO3a through a dynamic interplay with 14-3-3 and AKT. Mol Biol Cell. 2010 Mar 15; 21(6): ... The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. Nature. 2013 Jun 13; 498(7453):246-50. View in: ... Oncogenic Signaling Adaptor Proteins. J Genet Genomics. 2015 Oct 20; 42(10):521-529. View in: Pubmed ... Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens. Cell Syst. ...
Regulating proteosomal degradation of AD-related proteins, in the lab of Dr. Scott Wilson ... Role of 14-3-3 proteins in Alzheimers disease, in the lab of Dr. Talene Yacoubian ...
It is the protein which is left after milk is curdled and strained. There are plenty of whey protein supplements available in ... it is very probable that you find whey protein expensive and unaffordable or perhaps dont wish to try manufactured protein ... They may have been prescribed by your doctor or gym trainer to help you overcome your protein deficiency. However, ... Well, here are three vegetarian substitutes which can help you save the effort of buying expensive protein boxes. ...
... increase in APJ protein amounts in the spinal cord (Fig. 2B). Additionally, spinal apelin protein levels were significantly ... mRNA and protein levels were assessed at 3, 7 and 14 days post-injury; these time points corresponded, respectively, to the ... Masri B, Morin N, Pedebernade L, Knibiehler B and Audigier Y: The apelin receptor is coupled to Gi1 or Gi2 protein and is ... Western blot analysis presenting the time course for (A) apelin and (B) APJ protein expression in the spinal cord (n=6 in each ...
General protein information Go to the top of the page Help Preferred Names. protein disulfide-isomerase TMX3. Names. protein ... Protein interactions. Protein. Gene. Interaction. Pubs. Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 ... proteins that catalyze protein folding and thiol-disulfide interchange reactions. The canonical protein encoded by this gene ... Thioredoxin_like; Protein Disulfide Oxidoreductases and Other Proteins with a Thioredoxin fold. cl27119. Location:29 → 319. ...
These high-protein dinners are easy, tasty, and packed with micronutrients. They have helped a health reporter maintain her ... I followed a high-protein diet, ate at a gentle calorie deficit, and did strength training, but I didnt cut any food or drinks ... Im really into chili at the moment because its such a good source of protein, fiber, and micronutrients. I vary the toppings ... Here are three high-protein dinners that have become staples for me over the past year or two. ...
Protease-resistant prion protein (PrPres) was detected in the spleen and a cervical lymph node but not in the brain. He was ... Accumulation of prion protein in tonsil and appendix: review of tissue samples. BMJ. 2002 Sep 21. 325(7365):633-4. [QxMD ... 47] Whether PrPSc (prion protein, scrapie isoform) can be demonstrated in skeletal muscles remains controversial. [48, 49, 50, ... Prion protein (PrP) accumulation in the tonsil in variant Creutzfeldt-Jakob disease within follicular dendritic cells and ...
Per serving: 153 calories (percent of calories from fat, 75), 4 grams protein, 7 grams carbohydrates, 3 grams fiber, 14 grams ... When spinach has wilted, add 3 cloves roasted garlic, pepper and lemon juice. Reserve remainder of garlic and garlic-flavored ...
Baba W.N., Baby B., Mudgil P., Gan C.-Y., Vijayan R.* and Maqsood S.* (2021) Pepsin generated camel whey protein hydrolysates ... Jobe A., and Vijayan R. (2024) Orphan G protein-coupled receptors: the ongoing search for a home. Frontiers in Pharmacology 15: ... Ajayi F.F., Mudgil P., Jobe A., Antony P., Vijayan R., Gan C.Y., Maqsood S. (2023) Novel plant-protein (quinoa) derived ... Ali A., and Vijayan R. (2020) Dynamics of the ACE2-SARS-CoV-2/SARS-CoV spike protein interface reveal unique mechanisms. ...
Showing Protein Hemopexin (HMDBP01628). IdentificationBiological propertiesGene propertiesProtein propertiesExternal links ... Ratra R, Kar-Roy A, Lal SK: The ORF3 protein of hepatitis E virus interacts with hemopexin by means of its 26 amino acid N- ... Takahashi N, Takahashi Y, Putnam FW: Complete amino acid sequence of human hemopexin, the heme-binding protein of serum. Proc ... Bunkenborg J, Pilch BJ, Podtelejnikov AV, Wisniewski JR: Screening for N-glycosylated proteins by liquid chromatography mass ...
... daily protein intake much more than recommended - Featured Topics from the National Center for Health Statistics ... Protein intake contributes to calorie intake: therefore, if you eat more protein than is needed, your overall calorie intake ... Proteins can be found in a variety of foods that we eat on a regular basis and the table below displays the Centers for Disease ... Adults daily protein intake much more than recommended. Posted on March 3, 2010. by NCHS ...
Protein linked to Parkinsons also key for normal brain work: IIT Mandi study. * Thu, Feb 29 2024 12:56:58 PM ... Mandi, Feb 29 (IANS): A protein involved in the progression of Parkinsons disease also has a key role in regular brain ... It shows that this change, called phosphorylation at a certain site on the alpha-synuclein protein, is not just a disease ... Alpha-synuclein, like other proteins, being a polymer chain of amino acids, has a prominent phosphorylation site at the 129th ...
The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein ... Crystal structure of E.coli TPR-protein NlpI. 2bug. Solution structure of the TPR domain from Protein phosphatase 5 in complex ... Click on the protein counts, or double click on taxonomic names to display all proteins containing TPR domain in the selected ... Middle domain of Hsc70-interacting protein, crystal form I. 4j8f. Crystal structure of a fusion protein containing the NBD of ...
The ANCJDR has maintained a non-systematic approach to the prion protein genotyping of confirmed TSE cases. This may have ... Figure 3: Definite and probable TSE cases, 1970 to 2012, by aetiology and year. Year of Death. Sporadic. Familial. Iatrogenic. ... The CSF 14-3-3 protein test is now the most broadly utilized diagnostic tool for CJD. As of 2012, all CSF samples tested by the ... 1999; 3(4):311-315. *Will RG, Zeidler M, Stewart GE, Macleod MA, Ironside JW, Cousens SN, et al. Diagnosis of New Variant ...
  • 14-3-3 proteins have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. (wikipedia.org)
  • proteins that interact with 14-3-3 dimers include PKC and Raf, protein kinases with critical roles in neuronal signaling. (nih.gov)
  • We summarize current progress in identifying kinases that target membrane proteins with 14-3-3 binding sites and discuss the molecular mechanisms of 14-3-3 action. (manchester.ac.uk)
  • One of the kinases involved is Akt/PKB, which has recently been shown to activate the 14-3-3-dependent switch in a number of client membrane proteins. (manchester.ac.uk)
  • Arabidopsis Protein Kinases GRIK1 and GRIK2 Specifically Activate SnRK1 by Phosphorylating Its Activation Loop. (ncsu.edu)
  • Geminivirus infection up-regulates the expression of two Arabidopsis protein kinases related to yeast SNF1-and mammalian AMPK-activating kinases. (ncsu.edu)
  • Treating healthy seedlings and infected leaves with the proteasome inhibitor MG132 resulted in higher GRIK1 and GRIK2 protein levels, whereas treatment with the translation inhibitor cycloheximide reduced both kinases, demonstrating that their accumulation is modulated by posttranscriptional processes. (ncsu.edu)
  • Pseudokinases are considered to be the inactive counterparts of conventional protein kinases and comprise approximately 10% of the human and mouse kinomes. (elifesciences.org)
  • The SelO pseudokinases bind ATP with a flipped orientation relative to that of the ATP bound in the active site of canonical kinases and transfer the AMP moiety, instead of the γ-phosphate, from ATP to Ser, Thr, or Tyr residues on protein substrates. (elifesciences.org)
  • 14-3-3 binds to peptides. (wikipedia.org)
  • The geminivirus replication initiator protein (Rep) binds to the viral replication origin and catalyzes DNA cleavage and ligation to initiate rolling circle replication. (ncsu.edu)
  • An earlier study showed that a truncated version of GRIK1 binds to the geminivirus replication protein AL1. (ncsu.edu)
  • In mammalian cells 14-3-3 colocalizes and binds DNMT1pSer143 post-DNA replication. (pluto.bio)
  • A semisynthetic natural product derivative, DP-005, binds to an interface pocket of the p65/14-3-3 complex and concomitantly stabilizes it. (rcsb.org)
  • The study, published online this month in the journal Public Library of Science, shows that upon specific modification called phosphorylation, LRRK2 protein binds to a family of proteins called 14-3-3, which has a regulatory function inside cells. (sciencedaily.com)
  • The study, published online in PLoS ONE , shows that upon specific modification called phosphorylation, LRRK2 protein binds to a family of proteins called 14-3-3, which has a regulatory function inside cells. (sciencedaily.com)
  • Recent studies have shown that 14-3-3 binds to other proteins implicated in inherited Parkinson's disease and has a neuroprotective function, and when the binding is impaired due to these mutations, the protection may be lost. (sciencedaily.com)
  • These proteins contain a number of known common modification domains, including regions for divalent cation interaction, phosphorylation & acetylation, and proteolytic cleavage, among others established and predicted. (wikipedia.org)
  • Phosphorylation of Cdc25C by CDS1 and CHEK1 creates a binding site for the 14-3-3 family of phosphoserine binding proteins. (wikipedia.org)
  • Often, 14-3-3 binding to client proteins requires phosphorylation of the client, but the relevant kinase is unknown in most cases. (manchester.ac.uk)
  • Overexpression of a directed mutant of 14-3-3 omega in Arabidopsis leaves affects phosphorylation and protein content of nitrate reductase. (ncsu.edu)
  • In most cases, the binding was dependent on phosphorylation of the target protein, whereas that was not necessarily the case for binding to target proteins that were unaffected by polycations. (ncsu.edu)
  • To define the signal transduction pathways activated by MAPKAPK2, we identified potential MAPKAPK2 substrates by using a functional proteomic approach consisting of in vitro phosphorylation of neutrophil lysate by active recombinant MAPKAPK2, protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and phosphoprotein identification by peptide mass fingerprinting with matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) and protein database analysis. (korea.ac.kr)
  • The chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP) stimulated p38-MAPK-dependent phosphorylation of 14-3-3 proteins in human neutrophils. (korea.ac.kr)
  • Computational modeling and calculation of theoretical binding energies predicted that both phosphorylation at Ser-58 and mutation of Ser-58 to Asp (S58D) compromised the ability of 14-3-3ζ to dimerize. (korea.ac.kr)
  • These data suggest that MAPKAPK2-mediated phosphorylation regulates 14-3-3ζ functions, and this MAPKAPK2 activity may represent a novel pathway mediating p38 MAPK-dependent inflammation. (korea.ac.kr)
  • Using one-of-a-kind mouse models developed at Mount Sinai School of Medicine, Zhenyu Yue, PhD, Associate Professor of Neurology and Neuroscience, and his colleagues, found that several common Parkinson's disease mutations -- including one called G2019S -- disturb the specific phosphorylation of LRRK2.This impairs 14-3-3 binding with varying degrees, depending on the type of mutation. (sciencedaily.com)
  • Now that we know that phosphorylation is disturbed, causing 14-3-3 binding to be impaired, we have a new idea for diagnostic analysis and a new target for drug development. (sciencedaily.com)
  • Dr. Yue's team also identified a potential enzyme called protein kinase A (PKA), responsible for the phosphorylation of LRRK2. (sciencedaily.com)
  • The role of mitogen-activating protein kinase kinase kinase kinase-3 (MAP4K3) in preterm skeletal muscle satellite cells myogenesis or its relationship to mammalian target of rapamycin complex 1 (mTORC1) activity have not been previously elaborated. (medscimonit.com)
  • Several sequence analyses of these proteins have shown 4 regions with a high level of amino acid variability in loops I, V, VI, and VII (variable regions [VRs] 1-4) ( 2 ). (cdc.gov)
  • The porB gene sequence shows VR1-4, which is exclusive of PorB3 protein, and VR2-Eb, VR3-2ab, and VR4-Cc, which are typical of PorB2 (GenBank accession no. (cdc.gov)
  • The most likely origin of the porB2/3 hybrid (4, Eb, 2ab, Cc) is the acquisition of DNA that encodes a VR1-4 sequence by a meningococcus with a porB2-99 allelic variant. (cdc.gov)
  • Effect of mutations in the 14-3-3 sequence was predicted using its domain, secondary structure and multiple sequence alignment of amino acid sequences from wheat and its related species. (bvsalud.org)
  • See the reference protein sequence for serine/threonine-protein kinase PAK 4 isoform 1 (NP_005875.1). (nih.gov)
  • Seven genes encode seven distinct 14-3-3 proteins in most mammals (See Human genes below) and 13-15 genes in many higher plants, though typically in fungi they are present only in pairs. (wikipedia.org)
  • Eukaryotes can tolerate the loss of a single 14-3-3 gene if multiple genes are expressed, but deletion of all 14-3-3s (as experimentally determined in yeast) results in death. (wikipedia.org)
  • Most interestingly, mutations in the Drosophila 14-3-3 genes disrupt neuronal differentiation, synaptic plasticity, and behavioral plasticity, establishing a role for these proteins in the development and function of the nervous system. (nih.gov)
  • Genome derangement is frequently involved in carcinogenesis and may contribute to abnormalities in genes encoding proteins that have a critical role in key pathways related to cell growth and survival, leading to cancer development. (hindawi.com)
  • Binding of 14-3-3 has little effect on Cdc25C activity, and it is believed that 14-3-3 regulates Cdc25C by sequestering it to the cytoplasm, thereby preventing the interactions with CycB-Cdk1 that are localized to the nucleus at the G2/M transition. (wikipedia.org)
  • Therefore, we suggest that 14-3-3 is a crucial reader of DNMT1pSer143 that regulates DNMT1 level, DNA methylation and altered gene expression that contributes to cell invasion. (pluto.bio)
  • Experimentally, S58D mutation significantly impaired both 14-3-3ζ dimerization and binding to Raf-1. (korea.ac.kr)
  • There are common recognition motifs for 14-3-3 proteins that contain a phosphorylated serine or threonine residue, although binding to non-phosphorylated ligands has also been reported. (wikipedia.org)
  • In this study, we found that the Tomato golden mosaic virus (TGMV) Rep is phosphorylated at serine-97 by sucrose nonfermenting 1-related protein kinase 1 (SnRK1), a master regulator of plant energy homeostasis and metabolism. (ncsu.edu)
  • Nearly all geminivirus Rep proteins have a serine residue at the position equivalent to TGMV Rep serine-97. (ncsu.edu)
  • SnRK1: a versatile plant protein kinase that limits geminivirus infection. (ncsu.edu)
  • The plant protein kinase, SnRK1, has been implicated in host defenses against geminiviruses. (ncsu.edu)
  • GRIK, the SnRK1 activating kinase, is upregulated by geminivirus infection, while the viral C2 protein inhibits the SnRK1 activity. (ncsu.edu)
  • Sucrose Nonfermenting 1-Related Protein Kinase 1 Phosphorylates a Geminivirus Rep Protein to Impair Viral Replication and Infection. (ncsu.edu)
  • Sucrose nonfermenting 1-related protein kinase 1 targets the geminivirus Rep protein to interfere with viral infection. (ncsu.edu)
  • Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MAPKAPK2) mediates multiple p38 MAPK-dependent inflammatory responses. (korea.ac.kr)
  • The structure reveals that SidJ contains a protein kinase-like fold domain, which retains a majority of the characteristic kinase catalytic motifs. (elifesciences.org)
  • These proteins are capable of activating Raf in vitro, and this role has been verified by in vivo studies in Drosophila. (nih.gov)
  • As one approach to study this in-vivo role, we generated transgenic Arabidopsis plants constitutively overexpressing a directed mutant of 14-3-3 isoform ω that inhibits phosphorylated nitrate reductase (pNR) in a largely divalent-cation-independent manner in vitro. (ncsu.edu)
  • Binding of 14-3-3 proteins with DNMT1pSer143 resulted in inhibition of DNA methylation activity in vitro. (pluto.bio)
  • We confirmed that MAPKAPK2 interacted with and phosphorylated 14-3-3ζ in vitro and in HEK293 cells. (korea.ac.kr)
  • abstract = "14-3-3 proteins regulate the function and subcellular sorting of membrane proteins. (manchester.ac.uk)
  • Pentraxin 3/TSG-14 Protein, Human (HEK293, His) is the recombinant human-derived Pentraxin 3/TSG-14 protein, expressed by HEK293 , with C-10*His labeled tag and with natural variants A48D mutation. (medchemexpress.com)
  • Mutation analysis showed that MAPKAPK2 phosphorylated 14-3-3ζ at Ser-58. (korea.ac.kr)
  • When there is a mutation in LRRK2, 14-3-3 is impaired, leading to Parkinson's. (sciencedaily.com)
  • Here, we report the high-resolution crystal structures of two 14-3-3 binding motifs of p65 in complex with 14-3-3. (rcsb.org)
  • citation needed] 14-3-3 proteins were initially found in brain tissue in 1967 and purified using chromatography and gel electrophoresis. (wikipedia.org)
  • In bovine brain samples, 14-3-3 proteins were located in the 14th fraction eluting from a DEAE-cellulose column and in position 3.3 on a starch electrophoresis gel. (wikipedia.org)
  • Two-dimensional difference gel electrophoresis analysis of proteins extracted from leaves of plants expressing the mutant 14-3-3 identified numerous cellular proteins that were altered in abundance. (ncsu.edu)
  • The potential diagnostic value of these three proteins was subsequently characterized in one- and two-dimensional electrophoresis and Western blot to dot blot analyses, with Angiostrongylus-positive sera, normal human sera (NHS), and a pool of Echinococcus-positive sera (included as a specificity control) used for detection. (cdc.gov)
  • Crystallographic investigations have revealed that 14-3-3 proteins exist as dimers and that they contain a specific region for binding to other proteins. (nih.gov)
  • Early in the COVID-19 pandemic, it was quickly established that the receptor binding domain (RBD) of the SARS-CoV-2 spike protein is a prime target for neutralizing antibodies. (lbl.gov)
  • SnRK1 phosphorylated the equivalent serines in the Rep proteins of Tomato mottle virus and Tomato yellow leaf curl virus and reduced DNA binding, suggesting that SnRK1 plays a key role in combating geminivirus infection. (ncsu.edu)
  • Polycations globally enhance binding of 14-3-3 omega to target proteins in spinach leaves. (ncsu.edu)
  • The binding of 14-3-3omega to phosphorylated nitrate reductase (pNR) is stimulated by cations such as Mg(2+) or spermine, and decreased by 5'-AMP. (ncsu.edu)
  • In order to determine whether binding to other cellular proteins is affected similarly, far-Western overlays of extracts prepared from light- or dark-treated spinach (Spinacia oleracea) leaves were performed using digoxigenin (DIG)-labeled Arabidopsis 14-3-3omega. (ncsu.edu)
  • The presence of 5 mM Mg(2+) or 0.5 mM spermine enhanced binding to most of the target proteins to a maximum of about a doubling of the observed binding. (ncsu.edu)
  • In addition, binding of DIG-labeled 14-3-3omega to some, but not all, target proteins was reduced by 5 mM 5'-AMP. (ncsu.edu)
  • Interestingly, light/dark treatment of spinach leaves affected the subsequent binding of DIG-labeled 14-3-3omega in the overlay assay to only a few of the target proteins, one of which was identified as NADH:nitrate reductase. (ncsu.edu)
  • Overall, the results suggest that the binding of 14-3-3s to targets in addition to pNR may also be regulated by polycations and 5'-AMP. (ncsu.edu)
  • Here, we report the crystal structure of the Legionella pneumophila effector protein, SidJ, in complex with the eukaryotic Ca 2+ -binding regulator, calmodulin (CaM). (elifesciences.org)
  • it exhibits the strongest binding to APJ among all the isoforms ( 3 ). (spandidos-publications.com)
  • Results: Increased concentrations of beta-synuclein, gamma-synuclein, neurogranin, phosphatidylethanolamine-binding protein 1, and 14-3-3 proteins were observed in AD patients compared to controls, while neuronal pentraxin-2 and. (lu.se)
  • Goethe-Universita¨t, DE-60439 tein (I-LBP) and intestinal fatty acid-binding protein (I-FABP). (lu.se)
  • Hydration of Lipid-binding Proteins by ten anti-parallel b-strands. (lu.se)
  • 14-3-3 proteins are a family of conserved regulatory molecules that are expressed in all eukaryotic cells. (wikipedia.org)
  • 14-3-3 proteins bind to and sequester the transcriptional coregulators YAP/TAZ to the cytoplasm, inhibiting their function. (wikipedia.org)
  • Immobilized Anti-TSG-14 at 1 μg/mL (100 μL/well) can bind TSG-14. (medchemexpress.com)
  • Immobilized Anti-TSG-14 at 1 μg/mL (100 μL/well) can bind TSG-14 , The ED 50 for this effect is 0.04374 μg/mL. (medchemexpress.com)
  • His work helped generate detailed structural maps of how antibodies bind to the spike protein, enabling the selection of promising contenders. (lbl.gov)
  • The 14-3-3 family of proteins are highly conserved signaling proteins in eukaryotes that bind to their client proteins, usually through specific phosphorylated target sequences. (ncsu.edu)
  • Most of them encode proteins associated with cell survival responsiveness. (hindawi.com)
  • The level of DNMT1pSer143 increased with overexpression of 14-3-3 and decreased by its depletion. (pluto.bio)
  • In addition, overexpression of 14-3-3 in NIH3T3 cells led to decrease in DNMT1 specific activity and was rescued by transfection of DNMT1. (pluto.bio)
  • Furthermore, 14-3-3 overexpression also resulted in enhanced cell invasion. (pluto.bio)
  • Notably, Pentraxin 3/TSG-14 serves as a biomarker for several inflammatory conditions, reflecting its potential clinical significance. (medchemexpress.com)
  • The presence of 14-3-3 in spinal fluid is already used as a biomarker for the presence of neurodegenerative diseases. (sciencedaily.com)
  • Further applications of these findings could point to the use of 14-3-3 as a biomarker in testing for Parkinson's disease. (sciencedaily.com)
  • More than 200 signaling proteins have been reported as 14-3-3 ligands. (wikipedia.org)
  • Following the pairing of apelin peptides with cognate ligands, they have been identified to serve various roles in physiological and pathophysiological states, including effects on the cardiovascular system ( 6 - 8 ), fluid equilibrium ( 9 , 10 ), the adipoinsular signaling pathway ( 11 , 12 ), the immune system ( 13 ) and neuroprotection ( 14 ). (spandidos-publications.com)
  • LONP1 is an AAA+ protease that maintains mitochondrial homeostasis by removing damaged or misfolded proteins. (lbl.gov)
  • The total length of Pentraxin 3/TSG-14 Protein, Human (HEK293, His) is 364 a.a., with molecular weight of 44-50 kDa. (medchemexpress.com)
  • The extensive antigenic variability of these proteins forms the basis of the N. meningitidis serotyping scheme ( 3 , 4 ). (cdc.gov)
  • Heterologous expression studies using prokaryotic systems failed on producing antigenic proteins. (cdc.gov)
  • PorB outer membrane proteins are transmembrane proteins with 8 predicted surface-exposed loops (I-VIII), which vary in length and in amino acid sequences. (cdc.gov)
  • The presence of large gene families of 14-3-3 proteins in the Viridiplantae kingdom reflects their essential role in plant physiology. (wikipedia.org)
  • The Pentraxin 3/TSG-14 Protein, a member of the pentraxin protein family, is encoded by this gene. (medchemexpress.com)
  • Recently several reports have cleotide (designated as 0/10 bp) in the pro- focused on the association between the moter region of the gene at position -323 factor VII of the cascade coagulation and [ 13,14 ]. (who.int)
  • The impor- objectives of determining the frequencies tance of FVII in normal haemostasis is il- of these gene polymorphisms in the popula- lustrated by the severe bleeding diathesis tion and clarifying the genotype association associated with low plasma levels of the of the R353Q and 0/10 bp insertion poly- protein [ 3 ]. (who.int)
  • They are believed to interact with the protein Activation-Induced (Cytidine) Deaminase in mediating class switch recombination. (wikipedia.org)
  • While the 14-3-3 proteins are thought to interact with a wide array of cellular proteins, there have been few studies addressing the in-vivo role of 14-3-3. (ncsu.edu)
  • Membrane proteins as 14-3-3 clients in functional regulation and intracellular transport. (manchester.ac.uk)
  • for example, H-FABP makes up 3% of the plasmic intracellular LBPs from three different cytoplasmic protein content in rat heart. (lu.se)
  • While proteins are an important nutrient and you simply cannot do without them, sometimes you should listen to your body. (indiatimes.com)
  • One important nutrient is protein, which is essential to the human body because it is part of every cell, issue, and organ, allowing them to grow and repair. (cdc.gov)
  • Protein glutamylation is another type of ATP-dependent PTM, in which the γ-carboxyl group of a glutamate residue in a targeted protein is activated by ATP and then forms a isopeptide bond with the amino group of a free glutamate. (elifesciences.org)
  • A phylogenetic analysis of 27 plant species clustered the 14-3-3 proteins into four groups. (wikipedia.org)
  • The functional diversity of the protein in different species was correlated with mutations , change in secondary structure and the evolutionary relatedness of the protein in different species. (bvsalud.org)
  • This is the first novel work for analysing the mutational effect on the structure and function of a stress response protein (14-3-3) from Triticum aestivum and its related species. (bvsalud.org)
  • Protein crystallography is used for determining the molecular structure of proteins. (lbl.gov)
  • Therefore, identification of potential molecular biomarkers with high sensitivity and specificity would be beneficial for CCA diagnosis and patient prognosis as well as targeting therapeutics [ 8 , 14 ]. (hindawi.com)
  • Dysregulation of RGS5 Protein has been associated with various diseases, including hypertension and cardiovascular disorders. (medchemexpress.com)
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • 14-3-3 proteins activate the auto-inhibited plasma membrane P-type H+ ATPases. (wikipedia.org)
  • Crystallized protein molecules cause a beam of incident x-rays to scatter in many directions, with constructive and destructive interference generating a diffraction pattern. (lbl.gov)
  • Natural compounds are an important class of potent drug molecules including some retrospectively found to act as stabilizers of protein-protein interactions (PPIs). (rcsb.org)
  • Two tablespoons of homemade peanut butter equal eight grams of protein and are short of unhealthy fat as well. (indiatimes.com)
  • In addition, total NR protein was increased and the protein half-life was increased about two-fold. (ncsu.edu)
  • To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain. (wikipedia.org)
  • I lost 35 pounds by following a high-protein diet and strength training. (yahoo.com)
  • I followed a high-protein diet, ate at a gentle calorie deficit, and did strength training, but I didn't cut any food or drinks out of my diet entirely. (yahoo.com)
  • Here are three high-protein dinners that have become staples for me over the past year or two. (yahoo.com)
  • [ 3 ] The differential diagnosis of polyuria includes central diabetes insipidus, congenital or acquired nephrogenic diabetes insipidus, psychogenic polydipsia, high protein or hyperglycemic osmotic diuresis, salt-wasting nephropathies, mixed polyuria due to excess solute and water intake, and postobstructive diuresis following recovery from urinary obstruction. (medscape.com)
  • [ 3-14 ] Osmotic diuresis, driven by high protein intake, is typically encountered in hospitalized patients fed with exogenous protein administered by the enteral or parenteral route. (medscape.com)
  • Raf-1 Bad - see Bcl-2 Bax Cdc25 Akt SOS1 - see RSK YWHAB - "14-3-3 beta" YWHAE - "14-3-3 epsilon" YWHAG - "14-3-3 gamma" YWHAH - "14-3-3 eta" YWHAQ - "14-3-3 tau" YWHAZ - "14-3-3 zeta" SFN or YWHAS - "14-3-3 sigma" (Stratifin) The 14-3-3 proteins alpha and delta (YWHAA and YWHAD) are phosphorylated forms of YWHAB and YWHAZ, respectively. (wikipedia.org)
  • The aim of this study was to express and purify three recombinant glycoproteins representing A. cantonensis antigens: ES-7, Lec-5, and 14-3-3, in Chinese hamster ovary (CHO) cells and ES-7 in human embryonic kidney (HEK) cells to develop a source of specific antigens to be used in the diagnosis of angiostrongyliasis. (cdc.gov)
  • Method: Solid-phase extraction and parallel reaction monitoring mass spectrometry were used to quantify 17 synaptic proteins in CSF, in two cross-sectional studies including AD (n = 52) and controls (n = 37). (lu.se)
  • At AZTI, we design and formulate new products for this type of consumer by reducing animal protein intake and incorporating proteins from alternative sources in their composition (vegetable, microbial, marine, insect and meat substitutes). (azti.es)
  • Proteins can be found in a variety of foods that we eat on a regular basis and the table below displays the Centers for Disease Control and Prevention's recommended daily protein intake. (cdc.gov)
  • One of the eight candidate MAPKAPK2 substrates identified was the adaptor protein, 14-3-3ζ. (korea.ac.kr)
  • RGS5 Protein is a protein that plays a crucial role in the regulation of G-protein coupled receptor signaling. (medchemexpress.com)
  • This protein plays a crucial role in promoting fibrocyte differentiation and is actively involved in regulating inflammation and complement activation. (medchemexpress.com)
  • There is a crucial role of stress response proteins for the survival of the crops in stress conditions. (bvsalud.org)
  • Mutations and functional analysis of 14-3-3 stress response protein from Triticum aestivum: An evolutionary analysis through in silico structural biochemistry approach. (bvsalud.org)
  • The findings also demonstrate additional insight into the functional relevance of the LRRK2 and 14-3-3 interaction. (sciencedaily.com)
  • The retinoblastoma-related protein (RBR) is required for cell cycle control and differentiation and is expressed throughout the life of plants and animals. (ncsu.edu)
  • 14-3-3 has been reported to negatively regulate the p65 subunit of the NF-κB transcription factor, which qualifies this protein complex as a potential target for drug discovery to control cell proliferation. (rcsb.org)
  • G protein beta3 subunit 825T allele is associated with depression in young, healthy subjects. (bvsalud.org)
  • Discussion: We have established a method with a novel panel of synaptic proteins as biomarkers of synaptic dysfunction. (lu.se)
  • The results indicate that several of the proteins included in the panel may serve as synaptic biomarkers for AD. (lu.se)
  • Cooperativity analyses of this interaction, and other disease relevant 14-3-3-PPIs, demonstrated selectivity of DP-005 for the p65/14-3-3 complex. (rcsb.org)
  • Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma. (nih.gov)
  • citation needed] 14-3-3 proteins are structurally similar to the Tetratrico Peptide Repeat (TPR) superfamily, which generally have 9 or 10 alpha helices, and usually form homo- and/or hetero-dimer interactions along their amino-termini helices. (wikipedia.org)
  • Two scoops of whey protein contain approximately 35 grams of protein. (indiatimes.com)
  • In one go, your body can digest at the most 30 grams of protein. (indiatimes.com)
  • Other than this, protein quantity across brands differs, and it is not necessary that every scoop will give you 17 grams of proteins completely. (indiatimes.com)
  • CHICKPEAS: Every 100 grams of chickpea contains nine grams of protein. (indiatimes.com)
  • RAJMA OR KIDNEY BEANS: 100 grams of rajma contains anywhere between 22 grams to 24 grams of protein. (indiatimes.com)
  • The recommended daily amount of protein is 46 grams for women and 56 grams for men. (cdc.gov)
  • The fragment from nt 1 to 213 was identical to the porB3-193 allelic variant (VR1-4, VR2-Aa, VR3-7, VR4-14b), and the second part, with nt 233-972 identical to porB2-99 (VR1-Dc, VR2-Eb, VR3-2ab, VR4-Cc). The region of 214-232 nt is identical in the 3 variants. (cdc.gov)
  • A central chaperone-like role for 14-3-3 proteins in human cells. (nih.gov)
  • In this study, we analysed 14-3-3 protein , a stress response protein that is expressed in three major stresses, for example heat , drought and salinity and helps the plants to survive in those conditions. (bvsalud.org)
  • 14-3-3 proteins play an isoform-specific role in class switch recombination. (wikipedia.org)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • Protein-structure studies helped demonstrate that the primary target of antibody-based COVID-19 immunity is the part of the virus's spike protein that can most easily mutate. (lbl.gov)
  • 2,14 lation studies have examined cavity hydration in In the case of water, this has been confirmed by I-FABP. (lu.se)
  • However, porB2/3 recombinants have never been previously found in the nature. (cdc.gov)
  • Now, scientists have found a second region of the spike protein that is targeted by dozens of antibodies, some of which exhibit ultrapotent neutralizing activity. (lbl.gov)
  • [ 3 ] In contrast, no alterations in temporal or spatial EEG descriptors were found in normal aging. (medscape.com)
  • CCA is one of the highly aggressive malignant tumors [ 2 ] and has been reported as a major cause of death from the primary liver cancer [ 1 , 3 ]. (hindawi.com)