Muridae: A family of the order Rodentia containing 250 genera including the two genera Mus (MICE) and Rattus (RATS), from which the laboratory inbred strains are developed. The fifteen subfamilies are SIGMODONTINAE (New World mice and rats), CRICETINAE, Spalacinae, Myospalacinae, Lophiomyinae, ARVICOLINAE, Platacanthomyinae, Nesomyinae, Otomyinae, Rhizomyinae, GERBILLINAE, Dendromurinae, Cricetomyinae, MURINAE (Old World mice and rats), and Hydromyinae.Molecular Sequence Annotation: The addition of descriptive information about the function or structure of a molecular sequence to its MOLECULAR SEQUENCE DATA record.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Rodent Control: The reduction or regulation of the population of noxious, destructive, or dangerous rodents through chemical, biological, or other means.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Stroke: A group of pathological conditions characterized by sudden, non-convulsive loss of neurological function due to BRAIN ISCHEMIA or INTRACRANIAL HEMORRHAGES. Stroke is classified by the type of tissue NECROSIS, such as the anatomic location, vasculature involved, etiology, age of the affected individual, and hemorrhagic vs. non-hemorrhagic nature. (From Adams et al., Principles of Neurology, 6th ed, pp777-810)Astrocytes: A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.Brain Ischemia: Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.ReadingUp-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Cerebral Infarction: The formation of an area of NECROSIS in the CEREBRUM caused by an insufficiency of arterial or venous blood flow. Infarcts of the cerebrum are generally classified by hemisphere (i.e., left vs. right), lobe (e.g., frontal lobe infarction), arterial distribution (e.g., INFARCTION, ANTERIOR CEREBRAL ARTERY), and etiology (e.g., embolic infarction).Diffusion Magnetic Resonance Imaging: A diagnostic technique that incorporates the measurement of molecular diffusion (such as water or metabolites) for tissue assessment by MRI. The degree of molecular movement can be measured by changes of apparent diffusion coefficient (ADC) with time, as reflected by tissue microstructure. Diffusion MRI has been used to study BRAIN ISCHEMIA and tumor response to treatment.Intracranial Embolism: Blocking of a blood vessel in the SKULL by an EMBOLUS which can be a blood clot (THROMBUS) or other undissolved material in the blood stream. Most emboli are of cardiac origin and are associated with HEART DISEASES. Other non-cardiac sources of emboli are usually associated with VASCULAR DISEASES.Cerebrovascular Disorders: A spectrum of pathological conditions of impaired blood flow in the brain. They can involve vessels (ARTERIES or VEINS) in the CEREBRUM, the CEREBELLUM, and the BRAIN STEM. Major categories include INTRACRANIAL ARTERIOVENOUS MALFORMATIONS; BRAIN ISCHEMIA; CEREBRAL HEMORRHAGE; and others.14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Longevity: The normal length of time of an organism's life.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Fungal Proteins: Proteins found in any species of fungus.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Tuberous Sclerosis: Autosomal dominant neurocutaneous syndrome classically characterized by MENTAL RETARDATION; EPILEPSY; and skin lesions (e.g., adenoma sebaceum and hypomelanotic macules). There is, however, considerable heterogeneity in the neurologic manifestations. It is also associated with cortical tuber and HAMARTOMAS formation throughout the body, especially the heart, kidneys, and eyes. Mutations in two loci TSC1 and TSC2 that encode hamartin and tuberin, respectively, are associated with the disease.Angiomyolipoma: A benign tumor containing vascular, adipose, and muscle elements. It occurs most often in the kidney with smooth muscle elements (angiolipoleiomyoma) in association with tuberous sclerosis. (Dorland, 27th ed)Lymphangioleiomyomatosis: A disease characterized by the progressive invasion of SMOOTH MUSCLE CELLS into the LYMPHATIC VESSELS, and the BLOOD VESSELS. The majority of the cases occur in the LUNGS of women of child-bearing age, eventually blocking the flow of air, blood, and lymph. The common symptom is shortness of breath (DYSPNEA).Tumor Suppressor Proteins: Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.TOR Serine-Threonine Kinases: A serine threonine kinase that controls a wide range of growth-related cellular processes. The protein is referred to as the target of RAPAMYCIN due to the discovery that SIROLIMUS (commonly known as rapamycin) forms an inhibitory complex with TACROLIMUS BINDING PROTEIN 1A that blocks the action of its enzymatic activity.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Hypnosis, Dental: Process in which a patient is induced into a trance-like state in order to relieve anxiety during a dental procedure.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Rhabdomyoma: A benign tumor derived from striated muscle. It is extremely rare, generally occurring in the tongue, neck muscles, larynx, uvula, nasal cavity, axilla, vulva, and heart. These tumors are treated by simple excision. (Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1354)14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Dogs: The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Receptors, Adrenergic, alpha-2: A subclass of alpha-adrenergic receptors found on both presynaptic and postsynaptic membranes where they signal through Gi-Go G-PROTEINS. While postsynaptic alpha-2 receptors play a traditional role in mediating the effects of ADRENERGIC AGONISTS, the subset of alpha-2 receptors found on presynaptic membranes signal the feedback inhibition of NEUROTRANSMITTER release.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Calmodulin: A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.Receptors, Adrenergic, alpha-1: A subclass of alpha-adrenergic receptors that mediate contraction of SMOOTH MUSCLE in a variety of tissues such as ARTERIOLES; VEINS; and the UTERUS. They are usually found on postsynaptic membranes and signal through GQ-G11 G-PROTEINS.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Hemolysis: The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.False Positive Reactions: Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)Medical Laboratory Science: The specialty related to the performance of techniques in clinical pathology such as those in hematology, microbiology, and other general clinical laboratory applications.Medical Laboratory Personnel: Health care professionals, technicians, and assistants staffing LABORATORIES in research or health care facilities.Ice: The solid substance formed by the FREEZING of water.Laboratory Infection: Accidentally acquired infection in laboratory workers.Laboratories: Facilities equipped to carry out investigative procedures.Laboratories, Hospital: Hospital facilities equipped to carry out investigative procedures.Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
(1/1483) An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast.

The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well.  (+info)

(2/1483) Cell adhesion regulates the interaction between the docking protein p130(Cas) and the 14-3-3 proteins.

Integrin ligand binding induces a signaling complex formation via the direct association of the docking protein p130(Cas) (Cas) with diverse molecules. We report here that the 14-3-3zeta protein interacts with Cas in the yeast two-hybrid assay. We also found that the two proteins associate in mammalian cells and that this interaction takes place in a phosphoserine-dependent manner, because treatment of Cas with a serine phosphatase greatly reduced its ability to bind 14-3-3zeta. Furthermore, the Cas-14-3-3zeta interaction was found to be regulated by integrin-mediated cell adhesion. Thus, when cells are detached from the extracellular matrix, the binding of Cas to 14-3-3zeta is greatly diminished, whereas replating the cells onto fibronectin rapidly induces the association. Consistent with these results, we found that the subcellular localization of Cas and 14-3-3 is also regulated by integrin ligand binding and that the two proteins display a significant co-localization during cell attachment to the extracellular matrix. In conclusion, our results demonstrate that 14-3-3 proteins participate in integrin-activated signaling pathways through their interaction with Cas, which, in turn, may contribute to important biological responses regulated by cell adhesion to the extracellular matrix.  (+info)

(3/1483) Protein kinase C mu is negatively regulated by 14-3-3 signal transduction proteins.

Recent studies have documented direct interaction between 14-3-3 proteins and key molecules in signal transduction pathways like Ras, Cbl, and protein kinases. In T cells, the 14-3-3tau isoform has been shown to associate with protein kinase C theta and to negatively regulate interleukin-2 secretion. Here we present data that 14-3-3tau interacts with protein kinase C mu (PKCmu), a subtype that differs from other PKC members in structure and activation mechanisms. Specific interaction of PKCmu and 14-3-3tau can be shown in the T cell line Jurkat by immunocoprecipitiation and by pulldown assays of either endogenous or overexpressed proteins using PKCmu-specific antibodies and GST-14-3-3 fusion proteins, respectively. Using PKCmu deletion mutants, the 14-3-3tau binding region is mapped within the regulatory C1 domain. Binding of 14-3-3tau to PKCmu is significantly enhanced upon phorbol ester stimulation of PKCmu kinase activity in Jurkat cells and occurs via a Cbl-like serine containing consensus motif. However, 14-3-3tau is not a substrate of PKCmu. In contrast 14-3-3tau strongly down-regulates PKCmu kinase activity in vitro. Moreover, overexpression of 14-3-3tau significantly reduced phorbol ester induced activation of PKCmu kinase activity in intact cells. We therefore conclude that 14-3-3tau is a negative regulator of PKCmu in T cells.  (+info)

(4/1483) Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor.

Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/ threonine kinase Akt, which then phosphorylates and inactivates components of the apoptotic machinery, including BAD and Caspase 9. In this study, we demonstrate that Akt also regulates the activity of FKHRL1, a member of the Forkhead family of transcription factors. In the presence of survival factors, Akt phosphorylates FKHRL1, leading to FKHRL1's association with 14-3-3 proteins and FKHRL1's retention in the cytoplasm. Survival factor withdrawal leads to FKHRL1 dephosphorylation, nuclear translocation, and target gene activation. Within the nucleus, FKHRL1 triggers apoptosis most likely by inducing the expression of genes that are critical for cell death, such as the Fas ligand gene.  (+info)

(5/1483) Ca2+-induced apoptosis through calcineurin dephosphorylation of BAD.

The Ca2+-activated protein phosphatase calcineurin induces apoptosis, but the mechanism is unknown. Calcineurin was found to dephosphorylate BAD, a pro-apoptotic member of the Bcl-2 family, thus enhancing BAD heterodimerization with Bcl-xL and promoting apoptosis. The Ca2+-induced dephosphorylation of BAD correlated with its dissociation from 14-3-3 in the cytosol and translocation to mitochondria where Bcl-xL resides. In hippocampal neurons, L-glutamate, an inducer of Ca2+ influx and calcineurin activation, triggered mitochondrial targeting of BAD and apoptosis, which were both suppressible by coexpression of a dominant-inhibitory mutant of calcineurin or pharmacological inhibitors of this phosphatase. Thus, a Ca2+-inducible mechanism for apoptosis induction operates by regulating BAD phosphorylation and localization in cells.  (+info)

(6/1483) Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants.

Fusicoccin (FC) is a fungal toxin that activates the plant plasma membrane H+-ATPase by binding with 14-3-3 proteins, causing membrane hyperpolarization. Here we report on the effect of FC on a gene-for-gene pathogen-resistance response and show that FC application induces the expression of several genes involved in plant responses to pathogens. Ten members of the FC-binding 14-3-3 protein gene family were isolated from tomato (Lycopersicon esculentum) to characterize their role in defense responses. Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. Different specific subsets of 14-3-3 genes were induced after treatment with FC and during a gene-for-gene resistance response. Possible roles for the H+-ATPase and 14-3-3 proteins in responses to pathogens are discussed.  (+info)

(7/1483) Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import.

Cdc2-cyclin B1 in the G2-arrested Xenopus oocyte is held inactive by phosphorylation of Cdc2 at two negative regulatory sites, Thr14 and Tyr15. Upon treatment with progesterone, these sites are dephosphorylated by the dual specificity phosphatase, Cdc25, leading to Cdc2-cyclin B1 activation. Whereas maintenance of the G2 arrest depends upon preventing Cdc25-induced Cdc2 dephosphorylation, the mechanisms responsible for keeping Cdc25 in check in these cells have not yet been described. Here we report that Cdc25 in the G2-arrested oocyte is bound to 14-3-3 proteins and that progesterone treatment abrogates this binding. We demonstrate that Cdc25, apparently statically localized in the cytoplasm, is actually capable of shuttling in and out of the oocyte nucleus. Binding of 14-3-3 protein markedly reduces the nuclear import rate of Cdc25, allowing nuclear export mediated by a nuclear export sequence present in the N-terminus of Cdc25 to predominate. If 14-3-3 binding to Cdc25 is prevented while nuclear export is inhibited, the coordinate nuclear accumulation of Cdc25 and Cdc2-cyclin B1 facilitates their mutual activation, thereby promoting oocyte maturation.  (+info)

(8/1483) The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2-adrenergic receptor subtypes.

The alpha2-adrenergic receptors (alpha2ARs) are localized to and function on the basolateral surface in polarized renal epithelial cells via a mechanism involving the third cytoplasmic loop. To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. A protein doublet of approximately 30 kDa in Madin-Darby canine kidney cells or pig brain cytosol (alpha2B >/= alpha2C>> alpha2A) was identified. The interacting protein was purified by sequential DEAE and size exclusion chromatography, and subsequent microsequencing revealed that they are the zeta isoform of 14-3-3 proteins. [35S]Met-14-3-3zeta binds to all three native alpha2AR subtypes, assessed using a solid phase binding assay (alpha2A>/=alpha2B> alpha2C), and this binding depends on the presence of the 3i loops. Attenuation of the alpha2AR-14-3-3 interactions in the presence of a phosphorylated Raf-1 peptide corresponding to its 14-3-3 interacting domain (residues 251-266), but not by its non-phosphorylated counterpart, provides evidence for the functional specificity of these interactions and suggests one potential interface for the alpha2AR and 14-3-3 interactions. These studies represent the first evidence for G protein-coupled receptor interactions with 14-3-3 proteins and may provide a mechanism for receptor localization and/or coordination of signal transduction.  (+info)

*  14-3-3 protein
These proteins contain a number of known common modification domains, including regions for divalent cation interaction, ... More than 200 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and ... To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain.[ ... They are believed to interact with the protein Activation-Induced (Cytidine) Deaminase in mediating class switch recombination ...
*  CDC25B
The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has ... Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53-63. doi ... "Protein kinase CK2 regulates CDC25B phosphatase activity". Oncogene. England. 22 (2): 220-32. doi:10.1038/sj.onc.1206107. ISSN ... 14 (20): 2485-95. doi:10.1038/sj.onc.1201063. PMID 9188863. Booher RN, Holman PS, Fattaey A (1997). "Human Myt1 is a cell cycle ...
*  FOXO6
Forkhead box O6 is a protein that in humans is encoded by the FOXO6 gene. GRCm38: Ensembl release 89: ENSMUSG00000052135 - ... 5 (3): 233-40. doi:10.1111/1753-0407.12027. PMC 3657578 . PMID 23324123. Qinyu L, Long C, Zhen-dong D, Min-min S, Wei-ze W, Wei ... Tzivion G, Dobson M, Ramakrishnan G (November 2011). "FoxO transcription factors; Regulation by AKT and 14-3-3 proteins". ...
*  CDC25A
M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A is a protein that in humans is encoded by the ... In contrast, some invertebrates harbour 2 (e.g., the Drosophila proteins String and Twine) or four (e.g., C. elegans Cdc-25.1 ... Dual-specificity protein phosphatases remove phosphate groups from phosphorylated tyrosine and serine / threonine residues. ... Coqueret O, Bérubé G, Nepveu A (1998). "The mammalian Cut homeodomain protein functions as a cell-cycle-dependent ...
*  MAP3K2
Mitogen-activated protein kinase kinase kinase 2 is an enzyme that in humans is encoded by the MAP3K2 gene. The protein encoded ... Cheng J, Zhang D, Kim K, Zhao Y, Zhao Y, Su B (Jul 2005). "Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and ... This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a ... Yan M, Dai T, Deak JC, Kyriakis JM, Zon LI, Woodgett JR, Templeton DJ (1995). "Activation of stress-activated protein kinase by ...
*  G2-M DNA damage checkpoint
One such protein is rad18 that is required for G2 arrest even when chk1 is phosphorylated and active. This protein is also ... The cell cycle is driven by proteins called cyclin dependent kinases that associate with cyclin regulatory proteins at ... Proteins that localize to sites of DNA damage in the G2 phase initiate a signaling cascade that regulates wee1 and cdc25 ... Proteins that function in the G2-M checkpoint were originally identified in yeast screens that looked for mutants which show ...
*  KCNK18
... protein, human at the US National Library of Medicine Medical Subject Headings (MeSH). ... or K2P18.1 is a protein that in humans is encoded by the KCNK18 gene. K2P18.1 is a potassium channel containing two pore- ... Czirják G, Vuity D, Enyedi P (2008). "Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation". J. Biol ...
*  Telomerase reverse transcriptase
The protein can be a toxin, an apoptotic factor, or a viral protein. Toxins such as diphtheria toxin interfere with cellular ... "Regulation of telomerase activity and anti-apoptotic function by protein-protein interaction and phosphorylation". FEBS Lett. ... Viral proteins like viral thymidine kinase can be used for specific targeting of a drug. By introducing a prodrug only ... Song H, Li Y, Chen G, Xing Z, Zhao J, Yokoyama KK, Li T, Zhao M (April 2004). "Human MCRS2, a cell-cycle-dependent protein, ...
*  YWHAZ
This protein family is known for regulating signal transduction pathways primarily through binding phosphoserine proteins, ... is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. The protein encoded by this gene is a member of the ... though it can also bind phosphothreonine proteins and unphosphorylated proteins. By extension, 14-3-3 proteins are involved in ... "New insights into potential functions for the protein 4.1 superfamily of proteins in kidney epithelium". Frontiers in ...
*  C-Raf
Raf kinases A-Raf kinase B-Raf kinase KSR1 protein KSR2 protein GRCm38: Ensembl release 89: ENSMUSG00000000441 - Ensembl, May ... Yuryev A, Wennogle LP (February 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid ... a novel jun N-terminal protein kinase (JNK)-binding protein that functions as a Scaffold factor in the JNK signaling pathway". ... and the enzymes dedicated for removal of these phosphates are the protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A ...
*  Chloroplast DNA
... the protein import tunnel Toc75, plus the proteins Toc64 and Toc12. The first three proteins form a core complex that consists ... A protein kinase drifting around on the outer chloroplast membrane can use ATP to add a phosphate group to the Toc34 protein, ... This loss of GTP makes the Toc34 protein release the chloroplast preprotein, handing it off to the next TOC protein. Toc34 then ... Hundreds of different PPR proteins from the nuclear genome are involved in the RNA editing process. These proteins consist of ...
*  TP53
Mutant p53 proteins often fail to induce MDM2, causing p53 to accumulate at very high levels. Moreover, the mutant p53 protein ... In addition to the full-length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to ... One such example, human papillomavirus (HPV), encodes a protein, E6, which binds to the p53 protein and inactivates it. This ... The full length p53 isoform proteins can be subdivided into different protein domains. Starting from the N-terminus, there are ...
*  ASK1
MAP3K5 gene coding for the protein is located on chromosome 6 at locus 6q22.33. and the transcribed protein contains 1,374 ... "Entrez Gene: MAP3K5 mitogen-activated protein kinase kinase kinase 5". Yoon KW, Cho JH, Lee JK, Kang YH, Chae JS, Kim YM, Kim J ... Zama T, Aoki R, Kamimoto T, Inoue K, Ikeda Y, Hagiwara M (June 2002). "Scaffold role of a mitogen-activated protein kinase ... It activates c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases in a Raf-independent fashion in response ...
*  TFEB
"A helix-loop-helix protein related to the immunoglobulin E box-binding proteins". Molecular and Cellular Biology. 10 (8): 4384- ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ... Transcription factor EB is a protein that in humans is encoded by the TFEB gene. TFEB is a master gene for lysosomal biogenesis ... TFEB is a target of the protein kinase AKT/PKB. AKT/PKB phosphorylates TFEB at serine 467 and inhibits TFEB nuclear ...
*  BRAF (gene)
... while the protein is more formally known as serine/threonine-protein kinase B-Raf. The B-Raf protein is involved in sending ... B-Raf is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in ... Once B-Raf is activated, a conserved protein kinase catalytic core phosphorylates protein substrates by promoting the ... Hanks SK, Hunter T (May 1995). "Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain ...
*  KIF5B
Kinesin-1 heavy chain is a protein that in humans is encoded by the KIF5B gene. KIF5B has been shown to interact with: KLC1, ... Su Q, Cai Q, Gerwin C, Smith CL, Sheng ZH (Oct 2004). "Syntabulin is a microtubule-associated protein implicated in syntaxin ... Setou M, Seog DH, Tanaka Y, Kanai Y, Takei Y, Kawagishi M, Hirokawa N (May 2002). "Glutamate-receptor-interacting protein GRIP1 ... Hakimi MA, Speicher DW, Shiekhattar R (Oct 2002). "The motor protein kinesin-1 links neurofibromin and merlin in a common ...
*  KLC3
"Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi: ... Yang J, Liu X, Yue G, Adamian M, Bulgakov O, Li T (Nov 2002). "Rootletin, a novel coiled-coil protein, is a structural ... In the rat, a protein similar to this gene product is expressed in post-meiotic spermatids, where it associates with structural ... Identification and characterization of the encoded proteins". The Journal of Biological Chemistry. 273 (25): 15395-403. doi: ...
*  MST1
Macrophage-stimulating protein (MSP), also known as hepatocyte growth factor-like protein (HLP, HGFL, or HGFLP), is a protein ... and expression of human macrophage stimulating protein (MSP, MST1) confirms MSP as a member of the family of kringle proteins ... 2002). "Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells". J. Am. Soc. Nephrol. 13 (3 ... 1991). "Macrophage stimulating protein: purification, partial amino acid sequence, and cellular activity". J. Exp. Med. 173 (5 ...
*  MST1R
Macrophage-stimulating protein receptor is a protein that in humans is encoded by the MST1R gene. MST1R is also known as RON ( ... 1995). "Macrophage-stimulating protein activates Ras by both activation and translocation of SOS nucleotide exchange factor". ... Li BQ, Wang MH, Kung HF, Ronsin C, Breathnach R, Leonard EJ, Kamata T (November 1995). "Macrophage-stimulating protein ... Lee ST, Strunk KM, Spritz RA (1993). "A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes". ...
*  RRAD
GTP-binding protein RAD is a protein that in humans is encoded by the RRAD gene. RRAD has been shown to interact with CAMK2G ... Zhu J, Tseng YH, Kantor JD, Rhodes CJ, Zetter BR, Moyers JS, Kahn CR (1999). "Interaction of the Ras-related protein associated ... Finlin BS, Andres DA (1999). "Phosphorylation-dependent association of the Ras-related GTP-binding protein Rem with 14-3-3 ... and its regulation by a unique GTPase-activating protein (GAP)-like activity". J. Biol. Chem. 270 (9): 4805-12. doi:10.1074/jbc ...
*  HDAC7
This is most likely to allow for substrate recognition and protein-protein interactions that are necessary for class IIa HDAC ... The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous ... HDAC7A protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ... Class IIa HDACs are phosphorylated by calcium/calmodulin dependent-kindase (CaMK) and protein kinase D (PKD) in response to ...
*  PFTK1
2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... Serine/threonine-protein kinase PFTAIRE-1 is an enzyme that in humans is encoded by the PFTK1 gene. PFTK1 has been shown to ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 5 (6): 355-64. doi: ... "Entrez Gene: PFTK1 PFTAIRE protein kinase 1". Yang, Tao; Gao Yan-Kun; Chen Jiang-Ye (Jul 2002). "KIAA0202, a human septin ...
*  RPS6KA2
Ribosomal protein S6 kinase alpha-2 is an enzyme that in humans is encoded by the RPS6KA2 gene. This gene encodes a member of ... Du K, Montminy M (1999). "CREB is a regulatory target for the protein kinase Akt/PKB". J. Biol. Chem. 273 (49): 32377-9. doi: ... Lizcano JM, Morrice N, Cohen P (2001). "Regulation of BAD by cAMP-dependent protein kinase is mediated via phosphorylation of a ... Zhao, Y; Bjorbaek C; Moller D E (Nov 1996). "Regulation and interaction of pp90(rsk) isoforms with mitogen-activated protein ...
*  RIN1
2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ... Ras and Rab interactor 1 is a protein that in humans is encoded by the RIN1 gene. RIN1 has been shown to interact with HRAS. ... Han, L; Colicelli J (Mar 1995). "A human protein selected for interference with Ras function interacts directly with Ras and ... Han L, Colicelli J (1995). "A human protein selected for interference with Ras function interacts directly with Ras and ...
*  GP1BB
... protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... A 411 amino acid protein arising from a longer, unspliced transcript in endothelial cells has been described; however, the ... Longhurst CM, White MM, Wilkinson DA, Jennings LK (1999). "A CD9, alphaIIbbeta3, integrin-associated protein, and GPIb/V/IX ... Platelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain ...
14-3-3 proteins in cell cycle regulation.  - PubMed - NCBI  14-3-3 proteins in cell cycle regulation. - PubMed - NCBI
2006 Jun;16(3):183-92. Epub 2006 Apr 1. Research Support, Non-U.S. Gov't; Review ... M-transition by binding to regulatory proteins and modulating their function. In most cases, the association with 14-3-3 ... proteins requires a specific phosphorylation of the protein ligand and mediates cell cycle arrest. 14-3-3 binding may lead to ... Effects of 14-3-3 proteins on cell cycle progression and the regulation of 14-3-3 activity during the cell cycle are reviewed ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/16697662?dopt=Abstract
14-3-3 protein theta [Mus musculus] - Protein - NCBI  14-3-3 protein theta [Mus musculus] - Protein - NCBI
Protein expression data [Model Organism Protein Expres...] Protein expression data. Model Organism Protein Expression Database ... Transcript/Protein Information [PANTHER Classification System] Transcript/Protein Information. PANTHER Classification System ... Pre-computed sequence similarity results (BLAST) including alignments for the current protein against the NCBI nr protein ... MODBASE, Database of Comparative Protein Structure Models (Sali Lab/UCSF) [MODBASE, Database of Comparat...] MODBASE, Database ...
more infohttps://www.ncbi.nlm.nih.gov/protein/6756039
14-3-3 protein - Wikipedia  14-3-3 protein - Wikipedia
These proteins contain a number of known common modification domains, including regions for divalent cation interaction, ... More than 200 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and ... To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain.[ ... They are believed to interact with the protein Activation-Induced (Cytidine) Deaminase in mediating class switch recombination ...
more infohttps://en.wikipedia.org/wiki/14-3-3_protein
Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells | SpringerLink  Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells | SpringerLink
... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... of PC12 proteins and identified the TH protein as a major constituent of the proteins that are co-immunoprecipitated with 14-3- ... 5 cytoskelton proteins, 18 nuclear proteins including transcription factors, 10 metabolic enzymes, and others. We previously ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4757-3538-3_11
Frontiers | 14-3-3 Proteins in Guard Cell Signaling | Plant Science  Frontiers | 14-3-3 Proteins in Guard Cell Signaling | Plant Science
Reversible protein phosphorylation events are known to play a crucial role in the regulation of stomatal movements. However, in ... Among the phosphopeptide-binding proteins, the 14-3-3 proteins are the best characterized in plants. The 14-3-3s are found as ... Among the phosphopeptide-binding proteins, the 14-3-3 proteins are the best characterized in plants. The 14-3-3s are found as ... However, in some cases, phosphorylation alone is not sufficient to achieve complete protein regulation, but is necessary to ...
more infohttps://www.frontiersin.org/articles/10.3389/fpls.2015.01210/full
Structural basis for protein-protein interactions in the 14-3-3 protein family | PNAS  Structural basis for protein-protein interactions in the 14-3-3 protein family | PNAS
We suggest that the target protein is attracted first by general protein-protein interaction motifs (the desolvation patches) ... Structural basis for protein-protein interactions in the 14-3-3 protein family. Xiaowen Yang, Wen Hwa Lee, Frank Sobott, ... Approximately 2 μl of protein solution (10-30 μM) was loaded per capillary. Protein samples were buffer exchanged into 20 mM ... 4, where red patches represent likely protein-protein interaction interfaces. No low-desolvation patches were observed for any ...
more infohttps://www.pnas.org/content/103/46/17237?ijkey=d4da217073d5c8d2a7aadc991f9fb4af6288127c&keytype2=tf_ipsecsha
YWHAE - 14-3-3 protein epsilon - Homo sapiens (Human) - YWHAE gene & protein  YWHAE - 14-3-3 protein epsilon - Homo sapiens (Human) - YWHAE gene & protein
Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). ... Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ... to allow unambiguous identification of a protein.,p>,a href='/help/protein_names' target='_top'>More...,/a>,/p>Protein namesi. ... "Structural basis for protein-protein interactions in the 14-3-3 protein family.". Yang X., Lee W.H., Sobott F., Papagrigoriou E ...
more infohttps://www.uniprot.org/uniprot/P62258
YWHAQ - 14-3-3 protein theta - Homo sapiens (Human) - YWHAQ gene & protein  YWHAQ - 14-3-3 protein theta - Homo sapiens (Human) - YWHAQ gene & protein
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ... to allow unambiguous identification of a protein.,p>,a href='/help/protein_names' target='_top'>More...,/a>,/p>Protein namesi. ... "Structural basis for protein-protein interactions in the 14-3-3 protein family.". Yang X., Lee W.H., Sobott F., Papagrigoriou E ... PROSITE; a protein domain and family database. More...PROSITEi. View protein in PROSITE. PS00796 1433_1, 1 hit. PS00797 1433_ ...
more infohttps://www.uniprot.org/uniprot/P27348
RCSB PDB - Protein Feature View 









 - 14-3-3 protein zeta/delta - P63104 (1433Z HUMAN)  RCSB PDB - Protein Feature View - 14-3-3 protein zeta/delta - P63104 (1433Z HUMAN)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Protein Feature View of PDB entries mapped to a UniProtKB sequence * Number of PDB entries for Q6P3U9: no matching PDB entries ... This protein in other organisms (by gene name): Q6P3U9 - Homo sapiens 0 * Q32P43 - Homo sapiens no matching PDB entries ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ...
more infohttp://www.rcsb.org/pdb/protein/Q6P3U9
RCSB PDB - Protein Feature View 









 - 14-3-3 protein beta/alpha - P31946 (1433B HUMAN)  RCSB PDB - Protein Feature View - 14-3-3 protein beta/alpha - P31946 (1433B HUMAN)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... This protein in other organisms (by gene name): P31946 - Homo sapiens 11 * O70455 - Mus musculus no matching PDB entries ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a ...
more infohttp://www.rcsb.org/pdb/protein/P31946
14-3-3 protein gamma antibody | acris-antibodies.com  14-3-3 protein gamma antibody | acris-antibodies.com
14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase ... Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of ... An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.. 2. Aitken, A. et al. (2006) 14-3-3 ... Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.. *MAB2048 ...
more infohttps://www.acris-antibodies.com/target/14-3-3-protein-gamma-antibody.htm?ab_type=Ab%2FCP&images_t=ICC%2FIF&manufacturer=Acris+-+OriGene
14-3-3 protein gamma antibody | acris-antibodies.com  14-3-3 protein gamma antibody | acris-antibodies.com
14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase ... Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of ... An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.. 2. Aitken, A. et al. (2006) 14-3-3 ... Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.. *MAB2048 ...
more infohttps://www.acris-antibodies.com/target/14-3-3-protein-gamma-antibody.htm?ab_ag_reactivity=Chk&ab_host=Mouse&ab_type=Ab%2FCP&manufacturer=Acris+-+OriGene
Upregulated Expression of 14-3-3 Proteins in Astrocytes From Human Cerebrovascular Ischemic Lesions | Stroke  Upregulated Expression of 14-3-3 Proteins in Astrocytes From Human Cerebrovascular Ischemic Lesions | Stroke
Upregulated Expression of 14-3-3 Proteins in Astrocytes From Human Cerebrovascular Ischemic Lesions. Yasuhiro Kawamoto, Ichiro ... The American Heart Association is a qualified 501(c)(3) tax-exempt organization.. *Red Dress™ DHHS, Go Red™ AHA; National Wear ... Upregulated Expression of 14-3-3 Proteins in Astrocytes From Human Cerebrovascular Ischemic Lesions ... Upregulated Expression of 14-3-3 Proteins in Astrocytes From Human Cerebrovascular Ischemic Lesions ...
more infohttp://stroke.ahajournals.org/content/early/2006/01/19/01.STR.0000202587.63936.37
14-3-3 protein sigma | definition of 14-3-3 protein sigma by Medical dictionary  14-3-3 protein sigma | definition of 14-3-3 protein sigma by Medical dictionary
What is 14-3-3 protein sigma? Meaning of 14-3-3 protein sigma medical term. What does 14-3-3 protein sigma mean? ... Looking for online definition of 14-3-3 protein sigma in the Medical Dictionary? 14-3-3 protein sigma explanation free. ... A gene on chromosome 8q23.1 that encodes an adapter protein which regulates a broad spectrum of general and specialised ... When bound to KRT17, stratifin regulates protein synthesis and epithelial cell growth by stimulating the AKT/MTOR pathway. It ...
more infohttp://medical-dictionary.thefreedictionary.com/14-3-3+protein+sigma
Deleting the 14-3-3 Protein Bmh1 Extends Life Span in Saccharomyces cerevisiae by Increasing Stress Response | Genetics  Deleting the 14-3-3 Protein Bmh1 Extends Life Span in Saccharomyces cerevisiae by Increasing Stress Response | Genetics
Total protein extract was obtained as described (Easlon et al. 2008). About 15 μg of total proteins were loaded in each lane. ... Total protein extract was obtained by agitations using glass beads and the FastPrep beads beater. About 15 μg protein was ... Antibodies to total Bmh1 proteins (anti-Bmh1-total) and phosphorylated Bmh1 proteins (anti-Bmh1-pS238) were generated in ... Bmh proteins have been shown to interact with the retrograde response proteins (van Heusden and Steensma 2006), the stress- ...
more infohttp://www.genetics.org/content/183/4/1373
Regulation of tuberous sclerosis complex (TSC) function by 14-3-3 proteins | Biochemical Society Transactions  Regulation of tuberous sclerosis complex (TSC) function by 14-3-3 proteins | Biochemical Society Transactions
4E-binding protein 1; PKB, protein kinase B; TOR, target of rapamycin; FKHRL1, forkhead in rhabdosarcoma L1. ... and activating 4E-binding protein 1, an inhibitor of translation initiation. Phosphorylation-dependent binding between tuberin ... protein linase B (PKB)/target of rapamycin (TOR) signalling ... protein linase B (PKB)/target of rapamycin (TOR) signalling ... Regulation of tuberous sclerosis complex (TSC) function by 14-3-3 proteins. M. Nellist, M.A. Goedbloed, D.J.J. Halley ...
more infohttp://www.biochemsoctrans.org/content/31/3/587
The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2-adrenergic receptor...  The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2-adrenergic receptor...
To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the ... A protein doublet of approximately 30 kDa in Madin-Darby canine kidney cells or pig brain cytosol (alpha2B ,/= alpha2C, alpha2A ... To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the ... A protein doublet of approximately 30 kDa in Madin-Darby canine kidney cells or pig brain cytosol (alpha2B ,/= alpha2C, alpha2A ...
more infohttps://www.rti.org/publication/zeta-isoform-14-3-3-proteins-interacts-third-intracellular-loop-different-alpha2
Paracoccidoides brasiliensis 30 kDa Adhesin: Identification as a 14-3-3 Protein, Cloning and Subcellular Localization in...  Paracoccidoides brasiliensis 30 kDa Adhesin: Identification as a 14-3-3 Protein, Cloning and Subcellular Localization in...
These proteins are a conserved group of small acidic proteins involved in a variety of processes in eukaryotic organisms. ... suggesting that this protein may be involved in host-parasite interactions, since inhibition assays with the protein and this ... One of these proteins is a 30 kDa adhesin, pI 4.9 that was described as a laminin ligand in previous studies, and it was more ... This protein may contribute to the virulence of this important fungal pathogen. Using Edman degradation and mass spectrometry ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062533
Oscillating Aquaporin Phosphorylation and 14-3-3 Proteins Mediate the Circadian Regulation of Leaf Hydraulics | Plant Cell  Oscillating Aquaporin Phosphorylation and 14-3-3 Proteins Mediate the Circadian Regulation of Leaf Hydraulics | Plant Cell
The proteins were further analyzed by protein gel blot (protein gel blot; WB) analysis using anti-GRF4 [(A), top], anti-GRF10 [ ... Rosette Microsomal Protein Purification. The entire procedure for microsomal protein purification from rosettes was performed ... For protein gel blot analysis, proteins from microsomal extracts were separated by SDS-PAGE and transferred onto polyvinylidene ... Fusions of CBL-INTERACTING PROTEIN KINASE (CIPK10) protein kinase and AtPIP2;1 with NYFP were tentatively used as negative ( ...
more infohttp://www.plantcell.org/content/31/2/417
Phosphorylation of HopQ1, a Type III Effector from Pseudomonas syringae, Creates a Binding Site for Host 14-3-3 Proteins |...  Phosphorylation of HopQ1, a Type III Effector from Pseudomonas syringae, Creates a Binding Site for Host 14-3-3 Proteins |...
Recombinant HopQ1-6xHis protein obtained from E. coli was coincubated with leaf protein extracts from N. benthamiana or bean ( ... The protein mass and taxonomy were unrestricted, peptide mass tolerance was 20 ppm, and MS/MS tolerance was 0.8 D. Proteins ... enhanced cyan fluorescent protein. eYFP. enhanced yellow fluorescent protein. FLIM. fluorescence lifetime imaging microscopy. ... Wild-type HopQ1 protein carrying a C-terminal Flag epitope was transiently expressed in N. benthamiana leaves. Crude protein ...
more infohttp://www.plantphysiol.org/content/161/4/2049
  • citation needed] 14-3-3 proteins can be considered evolved members of the Tetratrico Peptide Repeat (TPR) superfamily, generally have 9 or 10 alpha helices, and usually form homo- and/or hetero-dimer interactions along their amino-termini helices. (wikipedia.org)
  • Binding of 14-3-3 has little effect on Cdc25C activity, and it is believed that 14-3-3 regulates Cdc25C by sequestering it to the cytoplasm, thereby preventing the interactions with CycB-Cdk1 that are localized to the nucleus at the G2/M transition. (wikipedia.org)
  • Attenuation of the alpha2AR-14-3-3 interactions in the presence of a phosphorylated Raf-1 peptide corresponding to its 14-3-3 interacting domain (residues 251-266), but not by its non-phosphorylated counterpart, provides evidence for the functional specificity of these interactions and suggests one potential interface for the alpha2AR and 14-3-3 interactions. (rti.org)
  • These studies represent the first evidence for G protein-coupled receptor interactions with 14-3-3 proteins and may provide a mechanism for receptor localization and/or coordination of signal transduction. (rti.org)
  • GRF10 (14-3-3Epsilon)] showed direct interactions with At PIP2;1 in the plant and upon coexpression in Xenopus laevis oocytes and activated At PIP2;1, preferentially when the latter was phosphorylated at its two C-terminal sites. (plantcell.org)
  • We report first non-covalent and exclusively extracellular inhibitors of 14-3-3 protein - protein interactions identified by virtual screening. (rsc.org)
  • The cellular 14-3-3 'pool' enables these proteins to react to altered signaling cues in an immediate and precise way through dynamic interactions with their clients. (elifesciences.org)
  • The ubiquitous eukaryotic 14-3-3 proteins coordinate multiple cellular processes due to their well-known regulatory function, which is based on specific recognition of phosphorylated motifs in their partners. (ovid.com)
  • 4. Nagy, G., Oostenbrink, C., and Hritz, J. Exploring the binding pathways of the 14-3-3ζ protein: Structural and free-energy profiles revealed by Hamiltonian replica exchange molecular dynamics with distancefield distance restraints. (findaphd.com)
  • citation needed] 14-3-3 proteins play an isoform-specific role in class switch recombination. (wikipedia.org)
  • Specifically, the structures of the beta isoform in its apo and peptide bound forms showed that its binding site can exhibit structural flexibility to facilitate binding of its protein and peptide partners. (pnas.org)
  • There are seven genes that encode seven distinct 14-3-3 proteins in most mammals (See Human genes below) and 13-15 genes in many higher plants, though typically in fungi they are present only in pairs. (wikipedia.org)
  • Blackledge, M. and Žídek, L.: Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. (findaphd.com)
  • Importantly, these activities are usually independent of substrate phosphorylation and therefore should be considered as distinct, 'moonlighting' functions of 14-3-3 proteins that mimic and complement the functions of dedicated molecular chaperones. (ovid.com)
  • More than 200 signaling proteins have been reported as 14-3-3 ligands. (wikipedia.org)
  • To identify proteins that may contribute to this retention, [35S]Met-labeled Gen10 fusion proteins with the 3i loops of the alpha2AAR (Val217-Ala377), alpha2BAR (Lys210-Trp354), and alpha2CAR (Arg248-Val363) were used as ligands in gel overlay assays. (rti.org)
  • These studies have revealed that 14-3-3 proteins can interact with the phosphorylated forms of their client proteins in response to certain signals, and that this binding finalizes the signaling event by enabling a change in the subcellular localization, protein stability or intrinsic enzymatic activity of the client, or by promoting an association between the client and other proteins. (elifesciences.org)
  • During the first hour of salt stress, the rate of proton extrusion from the excised roots increased in parallel with the ATP hydrolase activity and the amount of 14-3-3 proteins bound to H+-ATPase in isolated plasma membranes. (deepdyve.com)
  • To date, more than 70 proteins, localized in the cell membrane to nuclei, are reported to bind with the 14-3-3 family. (springer.com)
  • Cell cycle deregulation caused by changes in 14-3-3 expression has been implicated in cancer formation. (nih.gov)
  • Following transient expression in planta, HopQ1 was shown to copurify with host 14-3-3 proteins. (plantphysiol.org)
  • Thus, the expression of a specific bacterial effector can either sustain disease in susceptible plants or render the pathogen avirulent in resistant plants that express the corresponding R protein. (plantphysiol.org)
  • Blocking the interaction between PIF7 and 14-3-3 proteins or reducing the expression of 14-3-3 proteins accelerates shade-induced nuclear localization and de-phosphorylation of PIF7, and enhances the shade phenotype. (elifesciences.org)
  • Microscopic and flow cytometric analysis of cells with GFP-tagged SPL2 showed that disruption of BMH1 resulted in two populations of genetically identical cells, cells expressing the protein and the majority of cells with no detectible expression. (biomedcentral.com)
  • In these cells, expression of Cdc25C and Cdc25(S216A) is under the control of a hybrid protein consisting of a bacterial tetracycline repressor and VP16 activator protein ( 3 ). (sciencemag.org)
  • A ) Expression of Cdc25C and Cdc25(S216A) was induced by removal of tetracycline (Tet) from the media ( 3 ). (sciencemag.org)
  • Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. (lancs.ac.uk)
  • A comparative structural analysis of the seven 14-3-3 proteins revealed specificity determinants for binding of phosphopeptides in a specific orientation, target domain interaction surfaces and flexible adaptation of 14-3-3 proteins through domain movements. (pnas.org)
  • In addition, the complex of 14-3-3 beta with the exoenzyme S peptide displayed a secondary structural element in the 14-3-3 peptide binding groove. (pnas.org)
  • Optimization by crystal structure analysis and in vitro binding assays yielded compounds capable of disrupting the interaction of 14-3-3σ with aminopeptidase N in a cellular assay. (rsc.org)
  • Examples of well validated 14-3-3 interaction partners are the proto-oncogene RAF-1 ( 7 - 9 ) and the cell-cycle regulatory phosphatases Cdc25C/B ( 10 , 11 ). (pnas.org)
  • p>Manually curated information which has been propagated from a related experimentally characterized protein. (uniprot.org)
  • The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. (wikipedia.org)
  • Together, our studies suggest that phosphorylated Bmh1 may cause inhibitory effects on downstream longevity factors, including stress-response proteins. (genetics.org)
  • 14-3-3 binding may lead to cytoplasmic sequestration of the protein ligand but may also have other functional consequences. (nih.gov)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • The main neuropatological signs of Alzheimer disease are associated with the fibrillization of tau protein into neurofibrillar tangles. (findaphd.com)
  • The fibrillization of amyloid beta peptide into amyloid plaques and the agregation of hyperphosphorylated tau protein into neurofibrillar tangles are main neuropatological signs of Alzheimer disease. (findaphd.com)
  • 14-3-3 proteins function at several key points in G(1)/S- and G(2)/M-transition by binding to regulatory proteins and modulating their function. (nih.gov)
  • The physical interaction between HopQ1 and 14-3-3a was confirmed in planta using the fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy technique. (plantphysiol.org)
  • The 14-3-3 proteins can also recognize unmodified proteins such as the Pseudomonas aeruginosa virulence factor exoenzyme S (ExoS), p190RhoGEF, and the phage display-derived R18 peptide inhibitor ( 15 - 19 ). (pnas.org)
  • To date, the crystal structures of six classes of these proteins have been resolved and deposited in the public domain. (wikipedia.org)