Lipoxygenase: An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.Arachidonate 5-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 5-hydroperoxyarachidonate (5-HPETE) which is rapidly converted by a peroxidase to 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE). The 5-hydroperoxides are preferentially formed in leukocytes.Arachidonate 12-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). The 12-hydroperoxides are preferentially formed in PLATELETS.Lipoxygenase Inhibitors: Compounds that bind to and inhibit that enzymatic activity of LIPOXYGENASES. Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES.Arachidonate 15-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 15-hydroperoxyarachidonate (15-HPETE) which is rapidly converted to 15-hydroxy-5,8,11,13-eicosatetraenoate (15-HETE). The 15-hydroperoxides are preferentially formed in NEUTROPHILS and LYMPHOCYTES.Arachidonate Lipoxygenases: Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .12-Hydroxy-5,8,10,14-eicosatetraenoic Acid: A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)Leukotrienes: A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.Masoprocol: A potent lipoxygenase inhibitor that interferes with arachidonic acid metabolism. The compound also inhibits formyltetrahydrofolate synthetase, carboxylesterase, and cyclooxygenase to a lesser extent. It also serves as an antioxidant in fats and oils.Hydroxyeicosatetraenoic Acids: Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.5-Lipoxygenase-Activating Proteins: Scaffolding proteins that play an important role in the localization and activation of 5-LIPOXYGENASE.Arachidonic Acid: An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.Leukotriene B4: The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine: A dual inhibitor of both cyclooxygenase and lipoxygenase pathways. It exerts an anti-inflammatory effect by inhibiting the formation of prostaglandins and leukotrienes. The drug also enhances pulmonary hypoxic vasoconstriction and has a protective effect after myocardial ischemia.Arachidonic Acids5,8,11,14-Eicosatetraynoic Acid: A 20-carbon unsaturated fatty acid containing 4 alkyne bonds. It inhibits the enzymatic conversion of arachidonic acid to prostaglandins E(2) and F(2a).Leukotriene A4: (2S-(2 alpha,3 beta(1E,3E,5Z,8Z)))-3-(1,3,5,8-Tetradecatetraenyl)oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-HPETE via the stereospecific removal of a proton at C-10 and dehydration. Its biological actions are determined primarily by its metabolites, i.e., LEUKOTRIENE B4 and cysteinyl-leukotrienes. Alternatively, leukotriene A4 is converted into LEUKOTRIENE C4 by glutathione-S-transferase or into 5,6-di-HETE by the epoxide-hydrolase. (From Dictionary of Prostaglandins and Related Compounds, 1990)Linoleic Acids: Eighteen-carbon essential fatty acids that contain two double bonds.Eicosanoids: A class of compounds named after and generally derived from C20 fatty acids (EICOSANOIC ACIDS) that includes PROSTAGLANDINS; LEUKOTRIENES; THROMBOXANES, and HYDROXYEICOSATETRAENOIC ACIDS. They have hormone-like effects mediated by specialized receptors (RECEPTORS, EICOSANOID).SRS-A: A group of LEUKOTRIENES; (LTC4; LTD4; and LTE4) that is the major mediator of BRONCHOCONSTRICTION; HYPERSENSITIVITY; and other allergic reactions. Earlier studies described a "slow-reacting substance of ANAPHYLAXIS" released from lung by cobra venom or after anaphylactic shock. The relationship between SRS-A leukotrienes was established by UV which showed the presence of the conjugated triene. (From Merck Index, 11th ed)Lipid Peroxides: Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.Linoleic Acid: A doubly unsaturated fatty acid, occurring widely in plant glycosides. It is an essential fatty acid in mammalian nutrition and is used in the biosynthesis of prostaglandins and cell membranes. (From Stedman, 26th ed)Flavanones: A group of FLAVONOIDS characterized with a 4-ketone.Lipoxins: Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.Calcimycin: An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.Cyclooxygenase Inhibitors: Compounds or agents that combine with cyclooxygenase (PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES) and thereby prevent its substrate-enzyme combination with arachidonic acid and the formation of eicosanoids, prostaglandins, and thromboxanes.Indomethacin: A non-steroidal anti-inflammatory agent (NSAID) that inhibits the enzyme cyclooxygenase necessary for the formation of prostaglandins and other autacoids. It also inhibits the motility of polymorphonuclear leukocytes.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Leukotriene Antagonists: A class of drugs designed to prevent leukotriene synthesis or activity by blocking binding at the receptor level.Prostaglandin-Endoperoxide Synthases: Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor.Hydroxyurea: An antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.Leukotriene C4: The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Phospholipases A: Phospholipases that hydrolyze one of the acyl groups of phosphoglycerides or glycerophosphatidates.Receptors, Leukotriene: Cell-surface receptors that bind LEUKOTRIENES with high affinity and trigger intracellular changes influencing the behavior of cells. The leukotriene receptor subtypes have been tentatively named according to their affinities for the endogenous leukotrienes LTB4; LTC4; LTD4; and LTE4.Phospholipases A2: Phospholipases that hydrolyze the acyl group attached to the 2-position of PHOSPHOGLYCERIDES.Caffeic Acids: A class of phenolic acids related to chlorogenic acid, p-coumaric acid, vanillic acid, etc., which are found in plant tissues. It is involved in plant growth regulation.Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Linolenic Acids: Eighteen-carbon essential fatty acids that contain three double bonds.Leukocytes: White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).Leukotriene E4: A biologically active principle of SRS-A that is formed from LEUKOTRIENE D4 via a peptidase reaction that removes the glycine residue. The biological actions of LTE4 are similar to LTC4 and LTD4. (From Dictionary of Prostaglandins and Related Compounds, 1990)Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Benzeneacetamides: Compounds based on benzeneacetamide, that are similar in structure to ACETANILIDES.Fatty Acids, Unsaturated: FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.Reticulocytes: Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.8,11,14-Eicosatrienoic Acid: A 20-carbon-chain fatty acid, unsaturated at positions 8, 11, and 14. It differs from arachidonic acid, 5,8,11,14-eicosatetraenoic acid, only at position 5.Epoxide Hydrolases: Enzymes that catalyze reversibly the formation of an epoxide or arene oxide from a glycol or aromatic diol, respectively.Pyrazoles: Azoles of two nitrogens at the 1,2 positions, next to each other, in contrast with IMIDAZOLES in which they are at the 1,3 positions.Receptors, Leukotriene B4: A class of cell surface leukotriene receptors with a preference for leukotriene B4. Leukotriene B4 receptor activation influences chemotaxis, chemokinesis, adherence, enzyme release, oxidative bursts, and degranulation in polymorphonuclear leukocytes. There are at least two subtypes of these receptors. Some actions are mediated through the inositol phosphate and diacylglycerol second messenger systems.Thromboxane B2: A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).Oxylipins: Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Cucumis sativus: A creeping annual plant species of the CUCURBITACEAE family. It has a rough succulent, trailing stem and hairy leaves with three to five pointed lobes.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Lipoxygenases: Dioxygenases that catalyze the peroxidation of methylene-interrupted UNSATURATED FATTY ACIDS.Kinetics: The rate dynamics in chemical or physical systems.Autacoids: A chemically diverse group of substances produced by various tissues in the body that cause slow contraction of smooth muscle; they have other intense but varied pharmacologic activities.QuinolinesQuinacrine: An acridine derivative formerly widely used as an antimalarial but superseded by chloroquine in recent years. It has also been used as an anthelmintic and in the treatment of giardiasis and malignant effusions. It is used in cell biological experiments as an inhibitor of phospholipase A2.Dinoprostone: The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Peroxides: A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Solanum tuberosum: A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Benzoquinones: Benzene rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Cyclooxygenase 2: An inducibly-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes and INFLAMMATION. It is the target of COX2 INHIBITORS.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.5-Lipoxygenase-Activating Protein Inhibitors: Compounds that bind to and inhibit the action of 5-LIPOXYGENASE-ACTIVATING PROTEINS.Indoles: Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.Leukotriene D4: One of the biologically active principles of SRS-A. It is generated from LEUKOTRIENE C4 after partial hydrolysis of the peptide chain, i.e., cleavage of the gamma-glutamyl portion. Its biological actions include stimulation of vascular and nonvascular smooth muscle, and increases in vascular permeability. (From Dictionary of Prostaglandins and Related Compounds, 1990)Receptors, Eicosanoid: Cell surface proteins that bind eicosanoids with high affinity and trigger intracellular changes influencing the behavior of cells. Among the eicosanoid receptors are receptors for the prostaglandins, thromboxanes, and leukotrienes.Gas Chromatography-Mass Spectrometry: A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.Platelet Activating Factor: A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION.Cyclopentanes: A group of alicyclic hydrocarbons with the general formula R-C5H9.Intramolecular Oxidoreductases: Enzymes of the isomerase class that catalyze the oxidation of one part of a molecule with a corresponding reduction of another part of the same molecule. They include enzymes converting aldoses to ketoses (ALDOSE-KETOSE ISOMERASES), enzymes shifting a carbon-carbon double bond (CARBON-CARBON DOUBLE BOND ISOMERASES), and enzymes transposing S-S bonds (SULFUR-SULFUR BOND ISOMERASES). (From Enzyme Nomenclature, 1992) EC 5.3.Prostaglandins: A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.Nonheme Iron Proteins: Proteins, usually acting in oxidation-reduction reactions, containing iron but no porphyrin groups. (Lehninger, Principles of Biochemistry, 1993, pG-10)PyransUmbelliferones: 7-Hydroxycoumarins. Substances present in many plants, especially umbelliferae. Umbelliferones are used in sunscreen preparations and may be mutagenic. Their derivatives are used in liver therapy, as reagents, plant growth factors, sunscreens, insecticides, parasiticides, choleretics, spasmolytics, etc.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.ZymosanDose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Dioctyl Sulfosuccinic Acid: All-purpose surfactant, wetting agent, and solubilizer used in the drug, cosmetics, and food industries. It has also been used in laxatives and as cerumenolytics. It is usually administered as either the calcium, potassium, or sodium salt.Anti-Inflammatory Agents, Non-Steroidal: Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions.They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Prostaglandins E: (11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.Pleurisy: INFLAMMATION of PLEURA, the lining of the LUNG. When PARIETAL PLEURA is involved, there is pleuritic CHEST PAIN.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Pyrazolones: Compounds with a five-membered heterocyclic ring with two nitrogens and a keto OXYGEN. Some are inhibitors of TNF-ALPHA production.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Cyclooxygenase 1: A constitutively-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes.CycloheptanesIonophores: Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Eicosapentaenoic Acid: Important polyunsaturated fatty acid found in fish oils. It serves as the precursor for the prostaglandin-3 and thromboxane-3 families. A diet rich in eicosapentaenoic acid lowers serum lipid concentration, reduces incidence of cardiovascular disorders, prevents platelet aggregation, and inhibits arachidonic acid conversion into the thromboxane-2 and prostaglandin-2 families.Monocytes: Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.Enzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
... also termed arachidonate-5-lipoxygenase, 5-lipoxygenase, 5-LO, and 5-LOX). ALOX5 metabolizes arachidonic acid to its ... by the arachidonate 15-Lipoxygenase-1-based or arachidonate 15-lipoxygenased-2-based metabolism of 5-oxo-ETE; and f) conversion ... 12-Lipoxygenase (i.e. ALOX12) to metabolize 5(S)-HETE to 5(S),12(S)-diHETE. The activity of this product has not yet been fully ...
Epidermis-type lipoxygenase 3 (ALOXE3 or eLOX3) is a member of the lipoxygenase family of enzymes; in humans, it is encoded by ... The epidermis-type lipoxygenases are now regarded as a distinct subclass within the multigene family of mammalian lipoxygenases ... ALOXE3 arachidonate lipoxygenase 3". Schneider C, Brash AR (August 2002). "Lipoxygenase-catalyzed formation of R-configuration ... Among the human lipoxygenases, ALOXE3 is most closely (54% identity) related in amino acid sequence to ALOX12B. ALOXE3, ALOX12B ...
Among the human lipoxygenases, ALOX12B is most closely (54% identity) related in amino acid sequence to ALOXE3 ALOX12B ... The gene is located on chromosome 17 at position 13.1 where it forms a cluster with two other lipoxygenases, ALOXE3 and ALOX15B ... Muñoz-Garcia A, Thomas CP, Keeney DS, Zheng Y, Brash AR (March 2014). "The importance of the lipoxygenase-hepoxilin pathway in ... Zheng Y, Yin H, Boeglin WE, Elias PM, Crumrine D, Beier DR, Brash AR (July 2011). "Lipoxygenases mediate the effect of ...
J Neurosci (2002) 2000 : Hwang SW et al., Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous ... "Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous capsaicin-like substances". Proc. Natl. Acad ... 2002). "Bradykinin-12-lipoxygenase-VR1 signaling pathway for inflammatory hyperalgesia". Proc. Natl. Acad. Sci. USA. 99 (15): ... Nature (2008) 2002 : Shin J et al., Bradykinin-12-lipoxygenase-VR1 signaling pathway for inflammatory hyperalgesia. Proc Natl ...
... (also termed arachidonate 15-lipoxygenase, 15-lipoxygenase-1, 15-LO-1, 15-LOX-1) is, like other lipoxygenases, a seminal ... 15-lipoxygenase-2, 15-LOX-2, 15-LO-2, arachidonate 15-lipoxygenase type II, arachidonate 15-lipoxygenase, second type, and ... 15-LO is to be distinguished from another human 15-lipoxygenase enzyme, ALOX15B (also termed 15-lipoxygenase-2). Orthologs of ... Consequently, human ALOX15 is now referred to as arachidonate-15-lipoxygenase-1, 15-lipoxygenase-1, 15-LOX-1, 15-LO-1, human 12 ...
9 (1): 109-12. doi:10.1093/hmg/9.1.109. PMID 10587585. Al-Haggar M, Madej-Pilarczyk A, Kozlowski L, Bujnicki JM, Yahia S, Abdel ... 39 (12): 3185-91. doi:10.1021/bi992664v. PMID 10727209. Wilkinson FL, Holaska JM, Zhang Z, Sharma A, Manilal S, Holt I, Stamm S ... 93 (12): 4617-4623. doi:10.1210/jc.2008-0123. PMC 2626450 . PMID 18796515. Garg A, Cogulu O, Ozkinay F, Onay H, Agarwal AK ( ... 13 (12): 4401-13. doi:10.1091/mbc.E02-07-0450. PMC 138642 . PMID 12475961. Dechat T, Korbei B, Vaughan OA, Vlcek S, Hutchison ...
39 (12): 3185-91. doi:10.1021/bi992664v. PMID 10727209. Wu M, Xu LG, Zhai Z, Shu HB (2003). "SINK is a p65-interacting negative ... Tang K, Finley RL, Nie D, Honn KV (2000). "Identification of 12-lipoxygenase interaction with cellular proteins by yeast two- ...
39 (12): 3185-91. doi:10.1021/bi992664v. PMID 10727209. CS1 maint: Multiple names: authors list (link) Rogers MA, Winter H, ... Tang K, Finley RL, Nie D, Honn KV; Finley (2000). "Identification of 12-lipoxygenase interaction with cellular proteins by ...
... see Lipoxygenase#Biological function and classification#Human lipoxygenases) that also contains a very long chain fatty acid. ... Muñoz-Garcia, A; Thomas, C. P.; Keeney, D. S.; Zheng, Y; Brash, A. R. (2014). "The importance of the lipoxygenase-hepoxilin ... Muñoz-Garcia, A; Thomas, C. P.; Keeney, D. S.; Zheng, Y; Brash, A. R. (2014). "The importance of the lipoxygenase-hepoxilin ... Chawengsub, Y; Gauthier, K. M.; Campbell, W. B. (2009). "Role of arachidonic acid lipoxygenase metabolites in the regulation of ...
The enzymes 15-lipoxygenase-1 (15-LO-1 or ALOX15) and 15-lipoxygenase-2 (15-LO-2, ALOX15B) metabolize arachidonic acid to the S ... Other reactions of lipoxygenases generate cellular damage; murine models implicate 15-lipoxygenase in the pathogenesis of ... Lipoxygenases (LOXs): 5-Lipoxygenase (5-LOX or ALOX5) initiates the metabolism of arachidonic acid to 5- ... 15-lipoxygenase (15-lipoxygenase 1, 15-LOX, 15-LOX1, or ALOX15) initiates the metabolism of arachidonic acid to 15-HpETE, 15- ...
12 (10): 1293-9. doi:10.1038/nn.2379. PMID 19749751. Ryckmans T, Aubdool AA, Bodkin JV, Cox P, Brain SD, Dupont T, Fairman E, ... doi:10.1096/fj.12-217414. PMC 3633813 . PMID 23382512. Koivisto A, Chapman H, Jalava N, Korjamo T, Saarnilehto M, Lindstedt K, ... The eicosanoids formed in the ALOX12 (i.e. arachidonate-12-lipoxygnease) pathway of arachidonic acid metabolism, 12S- ... 12-oxido-5Z,9E,14Z-eicosatrienoic acid) and HxB3 (i.e. 10R/S-hydroxy-11,12-oxido-5Z,8Z,14Z-eicosatrienoic acid) (see Hepoxilin# ...
In addition, activated TH2 cells further release IL-4, leading to the up-regulation of the enzyme 15-lipoxygenase-1 (15-LO-1). ... "Selective survival rescue in 15-lipoxygenase-1-deficient retinal pigment epithelial cells by the novel docosahexaenoic acid- ... 12 (3): 432-437. doi:10.1039/c3ob41902a. Ogawa N, Kobayashi Y (June 2011). "Total synthesis of the antiinflammatory and ... Stein K, Stoffels M, Lysson M, Schneiker B, Dewald O, Krönke G, Kalff JC, Wehner S (2016). "A role for 12/15-lipoxygenase- ...
Takahashi, Y; Kawajiri, H; Yoshimoto, T; Hoshi, N; Higashida, H (1999). "12-Lipoxygenase overexpression in rodent NG108-15 ...
280 (36): 31405-12. doi:10.1074/jbc.M501489200. PMID 15987694. Ligresti A, Moriello AS, Starowicz K, Matias I, Pisanti S, De ... 8 (12): e81228. doi:10.1371/journal.pone.0081228. PMC 3857181 . PMID 24349046. Mabalirajan U, Rehman R, Ahmad T, Kumar S, Singh ... 12 (11): 545-54. doi:10.1016/j.molmed.2006.09.001. PMID 16996800. Pingle SC, Matta JA, Ahern GP (2007). "Capsaicin receptor: ... doi:10.1096/fj.12-217414. PMC 3633813 . PMID 23382512. Pace-Asciak CR (April 2015). "Pathophysiology of the hepoxilins". ...
Other lipoxygenases-8-LO, 12-LO and 15-LO-make other eicosanoid-like products. To act, 5-LO uses the nuclear-membrane enzyme 5- ... Lipoxygenase oxidation removes no C=C double bonds, and leads to the LK. After oxidation, the eicosanoids are further modified ... DGLA and EPA compete with AA for access to the cyclooxygenase and lipoxygenase enzymes. So the presence of DGLA and EPA in ... lipoxygenase-activating protein (FLAP), first to a hydroperoxyeicosatetraenoic acid (HPETE), then to the first leuokotriene, ...
The 5-lipoxygenase (5-LOX) pathway is implicated in the development and progression of human cancers. 5-LOX, whose crystal ... Arachidonate 5-lipoxygenase has been identified as playing a significant role in the survival of prostate cancer cells. ... Inhibition of 5-lipoxygenase by MK886 completely blocks 5-HETE production and induces massive apoptosis in both hormone- ... Ghosh J, Myers CE (October 1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate ...
Arachidonate 5-lipoxygenase Arachidonate 15-lipoxygenase Leukotriene 15-Hydroxyicosatetraenoic acid Greene ER, Huang S, Serhan ... The primary product of the lipoxygenase, 15-HPETE is believed to react with the enzyme further to produce the 14,15-epoxide, ... Claesson HE (September 2009). "On the biosynthesis and biological role of eoxins and 15-lipoxygenase-1 in airway inflammation ... Forsell PK, Brunnström A, Johannesson M, Claesson HE (2012). "Metabolism of anandamide into eoxamides by 15-lipoxygenase-1 and ...
12/15-Lipoxygenase contributes toPlatelet-Derived Growth Factor- Induced Activation of Signal Transducer and Activator of ... 107 (25): 11307-12. doi:10.1073/pnas.1000806107. ISSN 0027-8424. PMC 2895058 . PMID 20534510. ; rendered using PyMOL. Williams ...
... untranslated region of human glutathione peroxidase 4 influences lipoxygenase metabolism". Blood Cells Mol. Dis. 29 (2): 174-8 ... "Depletion of phospholipid hydroperoxide glutathione peroxidase up-regulates arachidonate metabolism by 12S-lipoxygenase and ... 16 (12): 1180-1191. doi:10.1038/ncb3064. Smith AC, Mears AJ, Bunker R, Ahmed A, MacKenzie M, Schwartzentruber JA, Beaulieu CL, ... "Glutathione peroxidase 4 senses and translates oxidative stress into 12/15-lipoxygenase dependent- and AIF-mediated cell death ...
Retrieved 2013-12-28. Elangovan S, D'Mello SR, Hong L, Ross RD, Allamargot C, Dawson DV, Stanford CM, Johnson GK, Sumner DR, ... Retrieved 2013-12-28. "Bio patch can regrow bone for dental implants and craniofacial defects". KurzweilAI. 2013-11-12. doi: ... 2011-10-12. Retrieved 2013-12-28. "New Stanford molecular target for diabetes treatment discovered - Office of Communications ... Heldin CH (1992). "Structural and functional studies on platelet-derived growth factor". EMBO J. 11 (12): 4251-4259. PMC 556997 ...
The flavonoid has been shown to inhibit certain types of lipoxygenases and act as an anti-inflammatory agent. It has ... 14 (12): 4295-301. doi:10.1016/j.bmc.2006.01.057. PMID 16500106. Hsieh CJ, Hall K, Ha T, Li C, Krishnaswamy G, Chi DS (2007). " ... 57 (12): 857-8. PMID 12561253. Hui KM, Wang XH, Xue H (2000). "Interaction of flavones from the roots of Scutellaria ... "Baicalein is a potent in vitro inhibitor against both reticulocyte 15-human and platelet 12-human lipoxygenases". Bioorg. Med. ...
... may refer to: Linoleate 8R-lipoxygenase, an enzyme 9,12-octadecadienoate 8-hydroperoxide 8R- ...
Leukotriene B4 (i.e. LTB4) is an arachidonic acid metabolite made by the 5-lipoxygenase enzyme pathway. It activates cells ... Anwar, Y; Sabir, J. S.; Qureshi, M. I.; Saini, K. S. (2014). "5-lipoxygenase: A promising drug target against inflammatory ... and lipoxygenase-derived eicosanoids". Drug Metabolism and Disposition. 39 (2): 180-90. doi:10.1124/dmd.110.035121. PMC 3033693 ... In 2009, 12-HHT was found to bind to the BLT2 receptor with ~10-fold higher affinity than LTB4; 12-HHT did not bind to the BLT1 ...
... see lipoxygenase#mouse lipoxygenases) are resistant to a) streptozotocin-induced, b) high fat diet-induced, and c) autoimmune- ... "CDNA cloning of a 8-lipoxygenase and a novel epidermis-type lipoxygenase from phorbol ester-treated mouse skin". Biochimica et ... Mice also express an epidermal type 15-lipoxygenase (e-12LO) which has 50.8% amino acid sequence identity to human 15-LOX-2 and ... Muñoz-Garcia, A; Thomas, C. P.; Keeney, D. S.; Zheng, Y; Brash, A. R. (2014). "The importance of the lipoxygenase-hepoxilin ...
Oxoeicosanoid receptor 1 is the receptor for a family of arachidonic acid metabolites made by 5-lipoxygenase viz., 5- ... G-protein coupled receptor 31 also known as 12-(S)-HETE receptor is a protein that in humans is encoded by the GPR31 gene. The ... The GPR31 receptor is a receptor for very different arachidonic acid metabolite, 12-hydroxyeicosatetraenoic acid (12-HETE), ... 12(S)-HETE stimulated the cloned GPR31 receptor to bind [35S]GTPγS with an EC50 (effective concentration causing a 50% of ...
March 2000, 39 (12): 3185-91. PMID 10727209. doi:10.1021/bi992664v.. *^ Wilkinson FL, Holaska JM, Zhang Z, Sharma A, Manilal S ... December 2006, 7 (12): 940-52. PMID 17139325. doi:10.1038/nrg1906.. *^ Rankin J, Ellard S. The laminopathies: a clinical review ... Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. Biochemistry. ... December 2002, 13 (12): 4401-13. PMC 138642. PMID 12475961. doi:10.1091/mbc.E02-07-0450.. ...
BioVision develops and offers a wide variety of products including assay kits, antibodies, recombinant proteins & enzymes, and other innovative research tools for studying Apoptosis, Metabolism, Cell Proliferation, Cellular Stress, Cell Damage and Repair, Diabetes, Obesity and Metabolic Syndrome, Stem Cell Biology, Gene Regulation, Signal Transduction, etc. BioVisions products are currently being sold in more than 60 countries worldwide.
Buy our Recombinant Human 12 Lipoxygenase protein. Ab112250 is a full length protein produced in Wheat germ and has been ... Belongs to the lipoxygenase family.. Contains 1 lipoxygenase domain.. Contains 1 PLAT domain. ... Recombinant Human 12 Lipoxygenase protein. See all 12 Lipoxygenase proteins and peptides. ...
Rabbit recombinant monoclonal 12 Lipoxygenase antibody [EPR11380(B)] validated for WB and tested in Human and Rat. Immunogen ... Belongs to the lipoxygenase family.. Contains 1 lipoxygenase domain.. Contains 1 PLAT domain. ... All lanes : Anti-12 Lipoxygenase antibody [EPR11380(B)] (ab168384) at 1/1000 dilution. Lane 1 : A431 lysate. Lane 2 : Human ... Anti-12 Lipoxygenase antibody [EPR11380(B)]. See all 12 Lipoxygenase primary antibodies. ...
Interleukin-4-dependent production of PPAR-gamma ligands in macrophages by 12/15-lipoxygenase.. Huang JT1, Welch JS, Ricote M, ... In monocytes and macrophages, 13-HODE and 15-HETE can be generated from linoleic and arachidonic acids, respectively, by a 12/ ... 15-lipoxygenase that is upregulated by the TH2-derived cytokine interleukin-4. Here we show that interleukin-4 also induces the ... These findings reveal a physiological role of 12/15-lipoxygenase in the generation of endogenous ligands for PPAR-gamma, and ...
Growth Cone Collapse Induced by Semaphorin 3A Requires 12/15-Lipoxygenase. Keith Mikule, Jesse C. Gatlin, Becky A. de la ... Growth Cone Collapse Induced by Semaphorin 3A Requires 12/15-Lipoxygenase. Keith Mikule, Jesse C. Gatlin, Becky A. de la ... Sema3A and 12(S)-HETE treatment of DRG growth cones causes a loss of adhesive area. IRM images were taken before (left) and ... Resting 12(S)-HETE levels were ∼11 fmol/μg protein (Table 1). This value is based on total protein in the cultures, rather than ...
Arachidonate 12-Lipoxygenase) Homo sapiens, Authors: Sreeparna Banerjee, Asli Erdog. Published in: Atlas Genet Cytogenet Oncol ... lipoxygenases together with 5-lipoxygenase are also particularly high in tumours from patients who died of breast cancer. ... Relationship between lipoxygenase and human testicular cancer.. Yoshimura R, Matsuyama M, Mitsuhashi M, Takemoto Y, Tsuchida K ... Arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) polymorphisms and colon cancer risk.. Goodman JE, Bowman ED, Chanock ...
Isolated liver macrophages were treated with 50 μM DHA and then infected by AAV-GFP or AAV-12-LOX. The CD206+/CD80+ ratio of F4 ... Seven-week-old WT male C57BL/6 mice were fed with HFD for 12 weeks. After 10 weeks of diet feeding, DHA was i.p. injected daily ... Levels of α-SMA, TGF-β, and 12-LOX proteins in the liver tissues were analyzed by Western blotting. (. G. ) The amount of MaR1 ... After 13 weeks of diet feeding, an intravenous injection of either AAV-GFP or AAV-12-LOX at 5 × 109 virus genomes was conducted ...
A role for lipoxygenase (LO)-derived products has been implicated in each of these events; therefore, we investigated the ... Evidence for the transformation of leukotriene A4 by platelet 12-lipoxygenase in vitro.. Serhan CN1, Sheppard KA. ... In cell-free 100,000 g supernatants of platelet lysates, which displayed 12-LO activity, LTA4 was also transformed to lipoxins ... interactions can lead to the formation of lipoxins from endogenous sources and provide a role for platelet 12-LO in the ...
ss-12-lox. Gene name: ALOX12. Species: pig (sus scorfa). Position of Oxygenation: 12 / 15. Substrate: arachidonic acid. ... Cloning and sequence analysis of the cDNA for arachidonate 12-lipoxygenase of porcine leukocytes.. Proc. Natl. Acad. Sci. U.S. ... Molecular structure and function of the porcine arachidonate 12-lipoxygenase gene.. J Biol Chem 267: 12188-12191 (1992). more ... studies on the iron-binding domain and the determinant for the substrate oxygenation site of porcine leukocyte arachidonate 12- ...
Platelet-Type 12-Lipoxygenase in a Human Prostate Carcinoma Stimulates Angiogenesis and Tumor Growth. Daotai Nie, Gilda G. ... In vitro, 12-LOX-transfected PC3 cells demonstrated a proliferation rate similar to neo controls. However, following s.c. ... Platelet-Type 12-Lipoxygenase in a Human Prostate Carcinoma Stimulates Angiogenesis and Tumor Growth ... Platelet-Type 12-Lipoxygenase in a Human Prostate Carcinoma Stimulates Angiogenesis and Tumor Growth ...
Bioactive lipids, especially cyclooxygenases and lipoxygenases (LOXs) metabolites of arachidonic acid, are known to be involved ... Additionally, 12-LOX and 12-HETE were induced in the glucose (30 mM) treated MDA-MB-231 cells and 12-HETE promoted MDA-MB-231 ... The levels of 12- LOX protein expression and its arachidonate metabolite 12-HETE were determined in both tumor tissues from the ... Abstract 3735: Dietary sugar induces tumorigenesis in mammary gland partially through 12 lipoxygenase pathway. Yan Jiang, Yong ...
arachidonate 12-lipoxygenase. Synonyms: 9930022G08Rik, Alox12p, P-12LO. Gene nomenclature, locus information, and GO, OMIM, and ... OMIM: ARACHIDONATE 12-OXIDOREDUCTASE; ALOX12*Gene Ontology: Alox12 *Mouse Phenome DB: Alox12 *UCSC: Chr.11:70,241,455- ... Vega: OTTMUSG6035 (Alox12, arachidonate 12-lipoxygenase)*CCDS: 24942, 24942.1* ...
Inhibition of platelet 12-lipoxygenase by hydroxy-fatty acids. PAUL D. MITCHELL, CATHERINE HALLAM, PAUL E. HEMSLEY, GARRY H. ... Inhibition of platelet 12-lipoxygenase by hydroxy-fatty acids. PAUL D. MITCHELL, CATHERINE HALLAM, PAUL E. HEMSLEY, GARRY H. ... Inhibition of platelet 12-lipoxygenase by hydroxy-fatty acids Message Subject (Your Name) has forwarded a page to you from ... g.c.-m.s., gas chromatography-mass spectrometry; 12-HETE, 12-ls-hydroxyeicosa-5z, 8z, 10e, 14z-tetraenoic acid; h.p.l.c., high- ...
Inhibition of 12/15-Lipoxygenase Protects Against β-Cell Oxidative Stress and Glycemic Deterioration in Mouse Models of Type 1 ... Inhibition of 12/15-Lipoxygenase Protects Against β-Cell Oxidative Stress and Glycemic Deterioration in Mouse Models of Type 1 ... Inhibition of 12/15-Lipoxygenase Protects Against β-Cell Oxidative Stress and Glycemic Deterioration in Mouse Models of Type 1 ... Inhibition of 12/15-Lipoxygenase Protects Against β-Cell Oxidative Stress and Glycemic Deterioration in Mouse Models of Type 1 ...
Alternatively, 5-lipoxygenase (5-LO) interacts with 5-lipoxygenase activating protein (FLAP), leading to the conversion of ... Synthesis of lipoxygenase metabolites. Synthesis of lipoxygenase metabolites begins with hydrolysis of arachidonic acid (AA) ... 5-lipoxygenase activating protein) with susceptibility to stroke (25,46,47). This calls further attention to the 5-lipoxygenase ... 5-Lipoxygenase has been noted to be expressed in some nonmyeloid cell types (42,43), and transcellular synthesis of ...
Inhibition of 12-LOX or PKC resulted in inhibition of dense granule secretion and attenuation of both aggregation and αIIbβ3 ... Furthermore, inhibition of 12-LOX had no effect on PKC-mediated aggregation indicating that 12-LOX is upstream of PKC. These ... Protein Kinase C Regulation of 12-lipoxygenase-mediated Human Platelet Activation. Jennifer Yeung, Patrick L Apopa, Joanne ... Protein Kinase C Regulation of 12-lipoxygenase-mediated Human Platelet Activation. Jennifer Yeung, Patrick L Apopa, Joanne ...
Lipoxygenase products have been shown to directly activate mitogen-activated protein kinases (MAPKs), and lipoxygenase pathway ... Superoxide generation by lipoxygenase in the presence of NADH and NADPH. Biochim Biophys Acta. 1994; 1214: 171-179. ... Lipoxygenase activity has been shown to be increased by and to mediate the hypertrophic effects of Ang II, the mitogenic ... Lipoxygenases and atherosclerosis: protection versus pathogenesis. Free Radical Biol Med. 2000; 28: 1726-1734. ...
The observation that 12/15-LOX-deficient (Alox15) mice developed a myeloid leukemia instigated my studies since leukemias are ... Therefore, I establish 12/15- LOX as a critical regulator of hematopoiesis and provide insight into novel mechanisms whereby ... Using genetic mouse models and in vitro and in vivo cell development assays, I found that 12/15-LOX indeed regulates multiple ... Moreover, my studies demonstrate that 12/15-LOX-mediated redox signaling of SHP-2 and the transcription factor ICSBP/IRF-8 ...
While most developed compounds primarily target the 5-lipoxygenase (5-LO) or the cyclooxygenase (COX) pathways, very few ... lipoxygenase (12-LO) pathway. The abundance of 12(S)-HETE and its contribution to several chronic inflammatory diseases have ... Identification of peracetylated quercetin as a selective 12-lipoxygenase pathway inhibitor in human platelets. Marco S Doucet, ... Identification of peracetylated quercetin as a selective 12-lipoxygenase pathway inhibitor in human platelets. Marco S Doucet, ...
Conclusion: 12/15-LO inhibition and statins like pravastatin may be useful as therapeutic strategies for prevention or ... Effects of pravastatin or 12/15 lipoxygenase pathway inhibitors on indices of diabetic nephropathy in an experimental model of ... the effects of early streptozotocin-induced diabetes on renal functions through supplementation of either pravastatin or 12/15- ...
Lipoxygenases (LOX) contribute to vascular disease and inflammation through generation of bioactive lipids, including 12-hydro( ... Platelet 12-Lipoxygenase Activation via Glycoprotein VI: Involvement of Multiple Signaling Pathways in Agonist Control of H(P) ... Human platelets that contain a 12-LOX isoform (p12-LOX) were used to define pathways that activate H(P)ETE synthesis in the ... Collagen and collagen-related peptide (CRP) (1 to 10 μg/mL) acutely induced platelet 12-H(P)ETE synthesis. This implicated the ...
We hypothesized that 12-LO in the pancreatic islet is sufficient to cause dysglycemia in the setting of high-fat feeding. To ... 12-lipoxygenase promotes obesity-induced oxidative stress in pancreatic islets.. Tersey, SA; Maier, B; Nishiki, Y; Maganti, AV ... 12-Lipoxygenase (12-LO) is activated by high-fat diets and catalyzes the oxygenation of cellular arachidonic acid to form ... At the molecular level, 12-LO deletion resulted in increases in islet antioxidant enzymes Sod1 and Gpx1 in response to high-fat ...
Beta4 interacts with 12-LOX, an enzyme that metabolizes arachidonic acid to yield 12(S)-HETE, a bioactive lipid that also ... Additionally, inhibition of 12-LOX led to decreased HGF-induced invasion. This is the first demonstration that 12-LOX may have ... However, despite 12-LOX recruitment, LC-MS analyses showed that HGF stimulation did not lead to increased 12(S)-HETE production ... 12-LOX knockdown with shRNA abolished HGF-induced beta4 phosphorylation and downstream signaling, leading to decreased HGF- ...
... and 15-lipoxygenases as well as the leukotriene receptors along the rat nephron. The kidneys were dissected into cortex and ... and 15-lipoxygenases and leukotriene receptors along the rat nephron. American Journal of Physiology: Renal Physiology 290 (4 ... the localization of lipoxygenases and leukotriene-forming enzymes together with leukotriene receptors in the kidney is less ... and 15-lipoxygenases and leukotriene receptors along the rat nephron ...
Manganese lipoxygenase. Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus). Loading... ...
  • The present studies on DRG neurons test the hypothesis that at least one of the Semas, Sema3A, also uses a signaling pathway involving 12/15-LO to trigger growth cone collapse and that this pathway affects growth cone adhesion. (jneurosci.org)
  • Two inhibitors recently identified by our groups through screening efforts, ML127 and ML351, have been shown to selectively target 12/15-LOX with high potency. (diabetesjournals.org)
  • In this study, we examined whether the distribution of hydroxyl groups among flavones could influence their potency as 12-LO inhibitors. (aspetjournals.org)
  • Objective: The present study aimed at attenuating the effects of early streptozotocin-induced diabetes on renal functions through supplementation of either pravastatin or 12/15-LO pathway inhibitors. (omicsonline.org)
  • To date few P-12-LOX inhibitors are known. (biomedcentral.com)
  • Immediately following these plenary lectures, three simultaneous breakout sessions were held, one of inflammation, a second on cancer and synthesis of novel inhibitors, and a third on enzymes-lipoxygenases/cyclooxygenases and inhibitors. (springer.com)
  • Jobard F, Lefèvre C, Karaduman A, Blanchet-Bardon C, Emre S, Weissenbach J, Ozgüc M, Lathrop M, Prud'homme JF, Fischer J. Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE) linked to chromosome 17p13.1. (nih.gov)
  • These studies demonstrate that 12/15-LO activity is a necessary step in Sema3A collapse signaling in growth cones and suggest a mechanism for its action. (jneurosci.org)
  • Moreover, my studies demonstrate that 12/15-LOX-mediated redox signaling of SHP-2 and the transcription factor ICSBP/IRF-8 promotes monocyte development while inhibiting granulocyte development. (upenn.edu)
  • Conclusions: These results demonstrate that 12/15LO mediates early stages of adipose tissue inflammation and whole body insulin resistance induced by high fat feeding. (elsevier.com)
  • 12/15-Lipoxygenase (12/15-LOX) is induced in β-cells and macrophages during T1D and produces proinflammatory lipids and lipid peroxides that exacerbate β-cell dysfunction and macrophage activity. (diabetesjournals.org)
  • This study characterizes a receptor-dependent pathway for 12-H(P)ETE synthesis via the collagen receptor GPVI, which is negatively regulated by PECAM-1 and PKC, and demonstrates a novel link between immune receptor signaling and lipid mediator generation in the vasculature. (ovid.com)
  • Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis. (biomedsearch.com)
  • F ) Levels of α-SMA, TGF-β, and 12-LOX proteins in the liver tissues were analyzed by Western blotting. (jci.org)
  • This is the first demonstration that 12-LOX may have a novel function in modulating c-MET and beta4 signaling through a scaffolding function and outlines a clear rationale to target all three proteins in cancer therapeutics. (wayne.edu)
  • Sequence data is available for the following lipoxygenases: Plants express a variety of cytosolic lipoxygenases (EC 1.13.11.12InterPro: IPR001246) as well as what seems to be a chloroplast isozyme. (wikipedia.org)
  • Thus, we tested the hypothesis that 12/15LO is involved in the onset of high fat diet (HFD)-induced insulin resistance. (elsevier.com)
  • Among them, prostaglandin endoperoxide synthases (cyclooxygenases) catalyze the committed step that leads to prostaglandin biosynthesis [ 12 - 14 ]. (hindawi.com)
  • We also show that the toxic actions of this biocide are zinc dependent and require the activation of p44/42 extracellular signal-regulated kinase (ERK) via a 12-lipoxygenase-mediated pathway. (jneurosci.org)
  • Background and Purpose- The current work is based on our previous finding that in neuronal cells, nmol/L concentrations of α-tocotrienol (TCT), but not α-tocopherol (TCP), blocked glutamate-induced death by suppressing early activation of c-Src kinase and 12-lipoxygenase. (ahajournals.org)
  • Our current work identifies two major targets of tocotrienol in the neuron: c-Src kinase and 12-lipoxygenase. (nih.gov)
  • Pharmacological as well as genetic approaches revealed that 12-Lox is rapidly tyrosine phosphorylated in the glutamate-challenged neuron and that this phosphorylation is catalyzed by c-Src. (ahajournals.org)
  • These findings reveal a physiological role of 12/15-lipoxygenase in the generation of endogenous ligands for PPAR-gamma, and suggest a paradigm for the regulation of nuclear receptor function by cytokines. (nih.gov)
  • These results indicate that neutrophil-platelet interactions can lead to the formation of lipoxins from endogenous sources and provide a role for platelet 12-LO in the formation of lipoxins from LTA4. (nih.gov)
  • Therefore, dietary sugar induced 12-LOX signaling may play an important role in increased incidence for breast cancer that is associated with a high-carbohydrate diet and could be a promising target for intervention and risk-reducing strategies for breast cancer. (aacrjournals.org)
  • We evaluated the role of 5- and 12-lipoxygenases in the development of diabetic retinopathy. (diabetesjournals.org)
  • To determine the role of PKC within the 12-LOX pathway, a number of biochemical endpoints were measured including platelet aggregation, calcium mobilization, and integrin activation. (aspetjournals.org)
  • The role of 12/15-LO in regulating VSMC proliferation is poorly understood. (ahajournals.org)
  • This study examines the role of Id3 in 12/15-LO-mediated VSMC proliferation. (ahajournals.org)
  • 9,12,15-17 Recently, its role in the VSMC has begun to be defined. (ahajournals.org)
  • Less data about platelet lipoxygenase pathway, especially for its biological role, are available. (springer.com)
  • As insulin resistance is closely associated with development of NAFLD, the role of 12-LOX in pathogenesis of NAFLD has received increasing attention in recent years. (elsevier.com)
  • In this review we discuss the role of 12-LOX in NAFLD pathogenesis and its potential role in emerging new therapeutics. (elsevier.com)
  • We identified a peracetylated quercetin (compound 6) that exert potent inhibitory activity towards the platelet 12-LO pathway (IC50 = 1.53 μM) while having a lesser affinity towards the COX pathway. (aspetjournals.org)
  • Methodology/Principal Findings: Cells over-expressing 12/15LO secreted two potent chemokines, MCP-1 and osteopontin, implicated in the development of insulin resistance. (elsevier.com)
  • Most notably, 5-lipoxygenase (LO) pathway products including LTC 4 and -D 4 , the slow-reacting substances of anaphylaxis, are considered causative agents in airway allergic responses, because they are potent bronchoconstrictors and inducers of leakage permeability changes ( 2 , 3 ). (jimmunol.org)
  • The initial 12(S)-HpETE and 12(R)-HpETE products, regardless of their pathway of formation, are rapidly reduced to 12(S)-HETE and 12(R)-HETE, respectively, by ubiquitous cellular peroxidases, including in particular Glutathione peroxidases or, alternatively, are further metabolized as described below. (wikipedia.org)