12-Hydroxy-5,8,10,14-eicosatetraenoic Acid: A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)Hydroxyeicosatetraenoic Acids: Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.Arachidonic AcidsLipoxygenase: An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.Arachidonate Lipoxygenases: Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .Leukotrienes: A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.Leukotriene B4: The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)Leukotriene A4: (2S-(2 alpha,3 beta(1E,3E,5Z,8Z)))-3-(1,3,5,8-Tetradecatetraenyl)oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-HPETE via the stereospecific removal of a proton at C-10 and dehydration. Its biological actions are determined primarily by its metabolites, i.e., LEUKOTRIENE B4 and cysteinyl-leukotrienes. Alternatively, leukotriene A4 is converted into LEUKOTRIENE C4 by glutathione-S-transferase or into 5,6-di-HETE by the epoxide-hydrolase. (From Dictionary of Prostaglandins and Related Compounds, 1990)Arachidonic Acid: An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.Prostaglandins B: Physiologically active prostaglandins found in many tissues and organs. They are potent pressor substances and have many other physiological activities.Arachidonate 5-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 5-hydroperoxyarachidonate (5-HPETE) which is rapidly converted by a peroxidase to 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE). The 5-hydroperoxides are preferentially formed in leukocytes.Arachidonate 12-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). The 12-hydroperoxides are preferentially formed in PLATELETS.Lipid Peroxides: Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.Lipoxygenase Inhibitors: Compounds that bind to and inhibit that enzymatic activity of LIPOXYGENASES. Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES.Eicosanoids: A class of compounds named after and generally derived from C20 fatty acids (EICOSANOIC ACIDS) that includes PROSTAGLANDINS; LEUKOTRIENES; THROMBOXANES, and HYDROXYEICOSATETRAENOIC ACIDS. They have hormone-like effects mediated by specialized receptors (RECEPTORS, EICOSANOID).Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Receptors, Leukotriene B4: A class of cell surface leukotriene receptors with a preference for leukotriene B4. Leukotriene B4 receptor activation influences chemotaxis, chemokinesis, adherence, enzyme release, oxidative bursts, and degranulation in polymorphonuclear leukocytes. There are at least two subtypes of these receptors. Some actions are mediated through the inositol phosphate and diacylglycerol second messenger systems.Gas Chromatography-Mass Spectrometry: A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.Hydroxy Acids: Organic compounds containing both the hydroxyl and carboxyl radicals.Calcimycin: An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.Fatty Acids, Unsaturated: FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.Seminal Vesicles: A saclike, glandular diverticulum on each ductus deferens in male vertebrates. It is united with the excretory duct and serves for temporary storage of semen. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)8,11,14-Eicosatrienoic Acid: A 20-carbon-chain fatty acid, unsaturated at positions 8, 11, and 14. It differs from arachidonic acid, 5,8,11,14-eicosatetraenoic acid, only at position 5.Peroxides: A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Microsomes: Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Chemotactic Factors: Chemical substances that attract or repel cells. The concept denotes especially those factors released as a result of tissue injury, microbial invasion, or immunologic activity, that attract LEUKOCYTES; MACROPHAGES; or other cells to the site of infection or insult.Prostaglandins: A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.Prostaglandin-Endoperoxide Synthases: Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Indomethacin: A non-steroidal anti-inflammatory agent (NSAID) that inhibits the enzyme cyclooxygenase necessary for the formation of prostaglandins and other autacoids. It also inhibits the motility of polymorphonuclear leukocytes.Eosinophils: Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Kinetics: The rate dynamics in chemical or physical systems.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).5,8,11,14-Eicosatetraynoic Acid: A 20-carbon unsaturated fatty acid containing 4 alkyne bonds. It inhibits the enzymatic conversion of arachidonic acid to prostaglandins E(2) and F(2a).Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Time Factors: Elements of limited time intervals, contributing to particular results or situations.

*Lipoxygenase

... arachidonic acid, linoleic acid, dihomo-γ-linolenic acid, and adrenic acid; the omega-3 fatty acids, eicosapentaenoic acid, ... Two lipoxygenases may act in series to make di-hydroxy or tri-hydroxy products that have activities quite different than either ... docosahexaenoic acid, and alpha-linolenic acid; and the omega-9 fatty acid, mead acid. Certain types of the lipoxygenases, e.g ... ALOX15 actually prefers linoleic acid over arachidonic acid, metabolizing linoleic acid to 12-hydroperoxyoctadecaenoic acid (13 ...

*15-Hydroxyeicosatetraenoic acid

Its 15(R) enantiomer is designated 15(R)-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid. Z and E give the cis-trans isomerism ... 13E-eicosatetraenoic acid (15(R)-HpETE). Human cells release arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid) from ... can be further metabolized to 15-oxo-eicosatetraenoic acid (15-oxo-ETE), 5S,15S-dihydroxy-eicosatetraenoic acid (5(S),15(S)- ... hydroxy-5Z,8Z,10E,13E-eicosatetraenoic acid (i.e. Eoxin C4 or EXC4) by leukotriene C4 synthase. EXC4 contains glutathione (i.e ...

*12-Hydroxyeicosatetraenoic acid

8R-hydroxy-11R,12R-epoxy-5Z,9E,14Z-eicosatetraenoic acid (i.e. 8R-hydroxy,11R,12R-epoxy-hepoxilin A3 or 8R-OH,11R,12R-epoxy- ... and trihydroxy-eicosatetraenoic acids that possess R or S hydroxy and epoxy residues at various sites while 8R-hydroxy,11R,12R- ... 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (12(S)-HETE or 12S-HETE), made by platelets, but also the later detected "R" ... hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (also termed 12(R)-HETE or 12R-HETE) made by other tissues through their 12R- ...

*List of MeSH codes (D10)

... oleic acids MeSH D10.251.355.325.600.525 --- oleic acid MeSH D10.251.355.325.600.780 --- ricinoleic acids MeSH D10.251.355.325. ... 15-hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic acid MeSH D10.251.355.255.100.637.100 --- prostaglandins a MeSH ... fatty acids, omega-6 MeSH D10.251.355.343.249 --- gamma-linolenic acid MeSH D10.251.355.343.500 --- linoleic acids MeSH D10.251 ... gamma-linolenic acid MeSH D10.251.355.840 --- sorbic acid MeSH D10.251.355.920 --- trans fatty acids MeSH D10.251.400.092 --- ...

*ALOX12

14Z-eicosatrienoic acid): 12S-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid → 8R/S-hydroxy-11,12-oxido-5Z,9E,14Z- ... 14Z-eicosatetraenoic acid) and O2 (i.e. oxygen) and its product is 12S-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (i.e. ... 13Z-eicosatetraenoic acid) and lipoxin B4 (5S,14R,15S-trihydroxy-6E,8Z,10E,12E-eicosatetraenoic acid); both lipoxins are SPMs ... metabolizes arachidonic acid primarily to 15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (i.e. 15(S)-HpETE; see 15- ...

*5-oxo-eicosatetraenoic acid

... hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HETE); and (d) the oxidation of 5(S)-HETE by a microsome-bound nicotinamide ... the release of arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid) from its storage sites in membrane phospholipids due ... Phospholipid-bound arachidonic acid → free arachidonic acid b) Free arachidonic acid + O2 → 5(S)-HpETE c) 5(S)-HpETE → 5(S)- ... and receptors of hydroxyeicosatetraenoic acids (HETEs) and oxoeicosatetraenoic acids (oxo-ETEs) derived from arachidonic acid ...

*ALOX15

14Z-eicosatrienoic acid) to 15S-hydroperoxy-8Z,11Z,13E-eicosatrienoic acid and 15S-hydroxy-8Z,11Z,13E-eicosatrienoic acid (15S- ... Conversion of arachidonic acid to 15-S-hydroperoxy-eicosatetraenoic acid". The Journal of Biological Chemistry. 257 (11): 6050- ... gamma-linolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid when presented not only as free acids ... By metabolizing the ω-3 polyunsaturated fatty acids, eicosapentaenoic acid and docosahexaenoic acid, into 17-HpDHA, 17-HDHA, ...

*5-Hydroxyeicosatetraenoic acid

... hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid. The Nobel laureate, Bengt I. Samuelsson, and colleagues first described 5(S)-HETE ... oxido-eicosatetraenoic acid viz., leukotriene A4 (i.e. S,6S-oxido-7E,9E,11Z,14Z-eicosatetraenoic acid). Leukotriene A4 may then ... arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid), by ALOX5 (also termed arachidonate-5-lipoxygenase, 5-lipoxygenase ... 14Z-eicosatetraenoic acid (5(S)-HpETE). 5-(S)-HpETE may then be release and rapidly converted to 5(S)-HETE by ubiquitous ...

*Eicosanoid

... hydroxy-eicosatetraenoic acid] is given the trivial names of 5S-HETE, 5(S)-HETE, 5S-HETE, or 5(S)-HETE). Since eicosanoid- ... The epoxy eicostrienoic acids (or EETs)-and, presumably, the epoxy eicosatetraenoic acids-have vasodilating actions on heart, ... The PUFA precursors to the eicosanoids include: Arachidonic acid (AA), i.e. 5Z, 8Z,11Z,14Z-eicosatetraenoic acid is ω-6 fatty ... Eicosapentaenoic acid (EPA), i.e.i.e. 5Z, 8Z,11Z,14Z,17Z-eicosapentaenoic acid is an ω-3 fatty acid with five cis double bonds ...

*Arachidonic acid

... β-hydroxy β-methylbutyric acid, and phosphatidic acid. Arachidonic acid is one of the most abundant fatty acids in the brain, ... Some chemistry sources define 'arachidonic acid' to designate any of the eicosatetraenoic acids. However, almost all writings ... Some arachidonic acid is converted into hydroxyeicosatetraenoic acids (HETEs) and epoxyeicosatrienoic acids (EETs) by ... Arachidonic acid (AA, sometimes ARA) is a polyunsaturated omega-6 fatty acid 20:4(ω-6). It is structurally related to the ...

*CYP4A11

Powell PK, Wolf I, Jin R, Lasker JM (Jun 1998). "Metabolism of arachidonic acid to 20-hydroxy-5,8,11, 14-eicosatetraenoic acid ... 20-epoxy-eicosapentaenoic acid isomers [i.e. 19,20-EDPs]) and eicosapentaenoic acid to epoxyeicosatetraenoic acids (EEQs, ... see 20-Hydroxyeicosatetraenoic acid, Epoxyeicosatetraenoic acid, and Epoxydocosapentaenoic acid sections on activities and ... CYP4A11 along with CYP4A22, CYP4F2, and CYP4F3 metabolize arachidonic acid to 20-Hydroxyeicosatetraenoic acid (20-HETE) by an ...

*Cytochrome P450 omega hydroxylase

... and docosahexaenoic acid (DHA). 20-hydroxy EPA and 20-hydroxy-DHA do stimulate Peroxisome proliferator-activated receptor alpha ... 4) Members of the CYP4F family, i.e. CYP4F2 and CYP4F3B, ω-hydroxylate very long chain fatty acids, i.e. fatty acids that are ... fatty acids that are 28 or more carbons long. The ω-hydroxylation of these special fatty acids is critical to creating and ... fatty acids that are 18 to 20 carbons long. These hydroxyl fatty acids are then serially metabolized by alcohol dehydrogenase, ...

*Oxoeicosanoid receptor 1

... hydroxy-6E,8E,11Z,14Z-eicosatetraenoic acid, and a series of synthetic mimetics of 5-oxo-ETE structure (compounds 346, S-264, S ... not only in its amino acid sequence but also in the hydroxy-carboxylic acid nature of its cognate ligands. Naturally occurring ... OXER1 is a G protein-coupled receptor (GPCR) that is structurally related to the hydroxy-carboxylic acid (HCA) family of G ... Recently, A G protein-couple receptor of the hydroxy carboxilic acid subfamily, Niacin receptor 1, has been proposed to mediate ...

*12-Hydroxyheptadecatrienoic acid

"Oxidation of 15-hydroxyeicosatetraenoic acid and other hydroxy fatty acids by lung prostaglandin dehydrogenase". Archives of ... 14Z-eicosatetraenoic acid (i.e. LTB4) but not LTB4's 12(S) or 6Z isomers while the BLT2 receptor exhibits a promiscuous finding ... 10E-heptadecatrienoic acid. Fourteen years after the first publication on its detection in 1973, 12-HHT was reported to ... hydroxy-5Z,8E,10E-heptadecatrienoic acid to indicate the S stereoisomerism of its 12-hydroxyl residue and the Z, E, and E cis- ...

*Epoxygenase

... see 20-Hydroxyeicosatetraenoic acid), i.e. 19-hydroxy- and/or 18-hydroxy-eicosatetranoic acids, takes on epoxygease activity in ... The metabolism of the straight chain 20-carbon polyunsaturated fatty eicosatetraenoic acid, arachidonic acid, by certain CYP ... However, the CYP epoxygenases also metabolize other omega-6 fatty acids such as linoleic acid and the omega-3 fatty acids, ... 14Z-eicosatetraenoic acid. Certain CYP epoxygenases attack these double bounds to form their respective eicosatrienoic acid ...

*TRPV1

Certain arachidonic acid metabolites, including 20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (see 20-Hydroxyeicosatetraenoic ... hydroxy-9Z,11E-octadecadienoic acid (13(S)-HODE), 13(R)-hydroxy-9Z,11E-octadecadienoic acid (13(R)-HODE, 9(S)-hydroxy-10(E),12( ... hydroxy-5Z,8Z,10E,12S,14Z-eicosatetraenoic acid (12(S)-HETE (see 12-HETE), hepoxilin A3 (i.e. 8R/S-hydroxy-11,12-oxido-5Z,9E, ... are metabolites of the omega 3 fatty acids, eicosapentaenoic acid (for RvE1) or docosahexaenoic acid (for RvD2, NPD1, and Mar1 ...

*Omega oxidation

... metabolize arachidonic acid (also known as eicosatetraenoic acid) to 20-hydroxyeicosatetreaneoic acid (20-HETE). 20-HETE ... which can enter the citric acid cycle, and adipic acid. The first step in ω-oxidation, i.e. addition of a hydroxy residue to ... and therapeutic modulation of 20-hydroxyeicosatetraenoic acid (20-HETE) synthase and fatty acid ω-hydroxylase activities". ... The steps of the process are as follows: After these three steps, either end of the fatty acid can be attached to coenzyme A. ...

*Docosahexaenoic acid

... elongation of the stearidonic acid by a Δ6 elongase to produce to eicosatetraenoic acid, desaturation at the fifth carbon of ... docosahexaenoyl amides of amino acids or neurotransmitters, and branched DHA esters of hydroxy fatty acids, among others. The ... eicosatetraenoic acid by a Δ5 desaturase to produce eicosapentaenoic acid, elongation of eicosapentaenoic acid by a Δ5 elongase ... DHA-clozapine List of omega-3 fatty acids Polyunsaturated fatty acids Guesnet P, Alessandri JM (2011). "Docosahexaenoic acid ( ...

*Arachidonate 5-lipoxygenase

5S-hydroxy,6R-(S-glutathionyl)-7E,9E,11Z,14Z-eicosatetraenoic acid). The Glu and Gly residues of LTC4 may be removed step-wise ... 6S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (i.e. LTA4). LTA4 is then acted on by a separate, soluble enzyme, Leukotriene-A4 ... hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (i.e. 5-HETE), or, alternatively, further metabolized by ALOX5's epoxidase (also ... ALOX5 metabolizes the omega-3 fatty acid, Eicosapentaenoic acid (EPA, i.e. 4Z,8Z,11Z,14Z,17Z-eiosapentaenoic acid), to 5- ...

*Specialized pro-resolving mediators

... pathway metabolizes arachidonic acid to 15S-hydroperoxy-eicosatetraenoic acid. The two aspirin-triggered lipoxins (AT-lipoxins ... n-3 DPA by COX2 to 13-hydroxy-DHA and 13-hydroxy-DPAn-3 products and by aspirin-treated COX2 to 17-hydroxy-DHA and 17-hydroxy- ... 16Z-docosapentaenoic acid or osbond acid) is an isomer of n-3 DPA (clupanodonic acid) differing form the latter fatty acid only ... SPM are metabolites of arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), or n-3 DPA (i.e. 7,10Z, ...

*Cytochrome P450

... and lipoxins to 20-hydroxy products. CYP4F22 ω-hydroxylates extremely long "very long chain fatty acids", i.e. fatty acids that ... eicosapentaenoic acid (i.e. EPA) to epoxyeicosatetraenoic acids (i.e. EEQs); and docosahexaenoic acid (i.e. DHA) to ... They also metabolize EPA to 19-hydroxyeicosapentaenoic acid (19-HEPE) and 20-hydroxyeicosapentaenoic acid (20-HEPE) as well as ... EETs to 20-hydroxy-EETs, and lipoxins to 20-hydroxy products. CYP4F8 and CYP4F12 metabolize PUFAs to signaling molecules: they ...

*TRPA1

... pathway of arachidonic acid metabolism, 12S-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (i.e. 12S-HpETE; see 12- ... 14Z-eicosatrienoic acid) and HxB3 (i.e. 10R/S-hydroxy-11,12-oxido-5Z,8Z,14Z-eicosatrienoic acid) (see Hepoxilin#Pain perception ... Resolvin D1 (RvD1) and RvD2 (see resolvinss) and maresin 1 are metabolites of the omega 3 fatty acid, docosahexaenoic acid. ... 14Z-eicosatetraenoic acid 4,5-Epoxyeicosatrienoic acid HC030031 GRC17536 A-967079 ALGX-2513 ALGX-2541 ALGX-2563 ALGX-2561 ALGX- ...

*Hepoxilin

13E-eicosatetraenoic acid → 11R-hydroxy-14S,15 S-epoxy-5Z,8Z,12E-eicosatrienoic acid and 13R-hydroxy-14S,15S-epoxy-5Z,8Z,11Z- ... 14Z-eicosatetraenoic acid + O2 → 12S-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid → 8R/S-hydroxy-11,12-oxido-5Z,9E,14Z- ... hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (i.e. 15S-HETE; see 15-hydroxyicosatetraenoic acid) may be involved in the ... those derived from the 22-carbon polyunsaturated fatty acid, docosahexaenoic acid, and the 18-carbon fatty acid, linoleic acid ...

*Lipoxin

13E-eicosatetraenoic acid LxB4: 5S,14R,15S-trihydroxy-6E,8Z,10E,12E-eicosatetraenoic acid 15-epi-LxA4: 5S,6R,15R-trihydroxy-7E, ... Structurally, they are defined as arachidonic acid metabolites that contain three hydroxyl residues (also termed hydroxy ... 9E,11Z,13E-eicosatetraenoic acid 15-epi-LxB4: 5S,14R,15R-trihydroxy-6E,8Z,10E,12E-eicosatrienoic acid Note that the two LXs ... which are metabolites of the omega 3 fatty acids, eicosapentaenoic acid or docosahexaenoic acid, as well as a range of ...

*Leukotriene B4 receptor 2

... see 15-Hydroxyicosatetraenoic acid), and by another member of the LTB4 family of arachidonic acid metabolites, 20-hydroxy-LTB4 ... 14Z-eicosatetraenoic acid (i.e. LTB4) but not LTB4's 12(S) or 6Z isomers whereas the BLT2 receptor exhibits a binding pattern ... Two BLT2-like receptors, Blt2a and Blt2b, with 49% amino acid identity to each other and 34% and 29%, respectively, amino acid ... Among these 7 ligand, in contrast, BLT1 binds and is activated by only LTB4 and 20-hydroxy-LTB4. The two BLT4-like receptors in ...

*Resolvin

... and docosahexaenoic acid (DHA) but also the docosapentaenoic acid (DPA), clupanodonic acid. They are members of an expanding ... hydroxy-DHA which is then transferred to nearby neutrophils which then use ALOX5 to oxygenate the 17(R)-hydroxyl-DHA to AT- ... osbond acid (i.e. 4Z,7Z,10Z,13Z,16Z-docosapentaenoic acid); the resolvin Dn-3DPA (RvDn-3DPA) are metabolites of the DPA isomer ... clupanodonic acid (i.e. 7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid); and the resolvin Ts (RvTs) are metabolites of clupanodonic ...
15-Hydroxyeicosatetraenoic acid (also termed 15-HETE, 15(S)-HETE, and 15S-HETE) is an eicosanoid, i.e. a metabolite of arachidonic acid. Various cell types metabolize arachidonic acid to 15(S)-hydroperoxyeicosatetraenoic acid (15(S)-HpETE). This initial hydroperoxide product is extremely short-lived in cells: if not otherwise metabolized, it is rapidly reduced to 15(S)-HETE. Both of these metabolites, depending on the cell type which forms them, can be further metabolized to 15-oxo-eicosatetraenoic acid (15-oxo-ETE), 5S,15S-dihydroxy-eicosatetraenoic acid (5(S),15(S)-diHETE), 5-oxo-15(S)-hydroxyeicosatetraenoic acid (5-oxo-15(S)-HETE, a subset of specialized pro-resolving mediators viz., the lipoxins, a class of pro-inflammatory mediators, the eoxins, and ...
Looking for online definition of 5,8,11,14-Eicosatetraenoic acid in the Medical Dictionary? 5,8,11,14-Eicosatetraenoic acid explanation free. What is 5,8,11,14-Eicosatetraenoic acid? Meaning of 5,8,11,14-Eicosatetraenoic acid medical term. What does 5,8,11,14-Eicosatetraenoic acid mean?
20-Hydroxyeicosatetraenoic acid (20-HETE) is a metabolite of arachidonic acid. Cytochrome P450 enzymes of the 4A and 4F families catalyze the omega-hydroxylation of arachidonic acid and produce 20-HETE. 20-HETE is a potent constrictor or renal, cerebral and mesenteric arteries. The vasoconstrictor response to 20-HETE is associated with activation of protein kinase, Rho kinase and the mitogen activated protein (MAP) kinase pathway C. 20-HETE also increases intracellular Ca2+ by causing the depolarization of vascular smooth muscle membrane secondary to blocking the large-conductance Ca2+-activated K+-channels and by a direct effect on L-type Ca channels. Elevations in the production of 20-HETE mediate the myogenic response of skeletal, renal and cerebral arteries to elevations in transmural pressure. There is an important interaction between nitric oxide (NO) and the formation of 20-HETE production. NO ...
22 20-hydroxyeicosatetraenoic acid (20-HETE) is the principal arachidonic acid metabolite in tubular and vascular structures of the rat kidney. In the tubules it inhibits sodium reabsorption, while in the renal microcirculation it is a vasoconstrictor and a regulator of myogenic tone. 20-HETE synthesis is catalyzed by the cytochrome P450 (CYP) 4A isoforms (4A1, 4A2, 4A3 and 4A8). Our studies indicated that despite the high homology, these isoforms display distinct catalytic properties. CYP4A1 is the low Km isoform and thus, by far, the most efficient 20-HETE synthesizing enzyme. Whereas CYP4A1 is solely an ω-hydroxylase, CYP4A2 and CYP4A3 also catalyze arachidonate 11,12-epoxidation. Systemic administration of CYP4A1 antisense oligodeoxynucleotides reduced the level of CYP4A proteins and 20-HETE synthesis in renal vessels by 50%, and decreased blood pressure in SHR from 137±3 to 121±4 mmHg (p , 0.05). Immunoblot analysis and ...
Recent studies have indicated that renal arteries can produce 20-hydroxyeicosatetraenoic acid (20-HETE) and suggest the potential involvement of a P450 metabolite of arachidonic acid in the myogenic activation of canine renal arteries. In the present study, the effects of 20-HETE on isolated canine renal arcuate arteries were studied. Administration of 20-HETE to the bath or the lumen at concentrations of 0.01-1 microM produced a graded reduction in the diameter of these vessels. In contrast, 19(R)-HETE was a vasodilator, whereas 19(S)-HETE was relatively inactive. The vasoconstrictor response to 20-HETE was not altered by the cyclooxygenase inhibitor indomethacin, endoperoxide/thromboxane receptor antagonist SQ29548, or combined blockade of the cyclooxygenase, lipoxygenase, and P450 pathways using indomethacin, baicalein, and 7-ethoxyresorufin. The response to 20-HETE was associated with depolarization and a sustained increase in the ...
Looking for 5,8,11,14-Eicosatetraenoic acid? Find out information about 5,8,11,14-Eicosatetraenoic acid. C20H32O2 An essential unsaturated fatty acid that is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes Explanation of 5,8,11,14-Eicosatetraenoic acid
LOXs form a heterogeneous family of lipid-peroxidizing enzymes that introduce molecular oxygen into PUFA such as AA and linoleic acid (LA). Based on their ability to oxidize the corresponding carbon atom of AA they are classified as 5-, 8-, 12-, and 15-LOXs.8 A large body of literature suggests that LOXs, including 15-LOX, play a role in the pathogenesis of vessel wall diseases.10,11,14 However, the mechanism(s) of their action is not very clear. Therefore, here we studied the effect of 15(S)-HETE, a product of 15-LOX1/2, on VSMC migration and explored the underlying mechanisms. We observed that: (1) Among the 3 LOX products of AA tested, 15(S)-HETE was found to be most potent in inducing VSMC migration; (2) 15(S)-HETE induced CREB phosphorylation in a time-dependent manner and adenovirus-mediated expression of its dominant-negative mutant inhibited 15(S)-HETE-induced VSMC migration; (3) 15(S)-HETE stimulated the phosphorylation of ERK1/2, JNK1/2, and p38MAPK in a time-dependent manner; (4) ...
P164 Angiotensin II (Ang II) promotes arachidonic acid (AA) release via activation of cytosolic phospholipase A2 (cPLA2) and D (PLD) in rabbit vascular smooth muscle cells (VSMC). Ang II also stimulates the Ras/MAP kinase pathway, and MAP kinase activates cPLA2 and PLD in VSMC. The increase in Ras/MAP kinase activity elicited by Ang II in VSMC is mediated by 20-hydroxyeicosatetraenoic acid (HETE) and to a lesser extent by 12(S)-HETE, metabolites of AA generated by cytochrome P450 (CYP) and lipoxygenase (LO), respectively, upon activation of cPLA2 by calcium calmodulin-dependent kinase II. The purpose of this study was to determine if Ang II-induced PLD activation stimulation in VSMC from rabbit aorta is mediated by the Ras/MAP kinase pathway via AA metabolites generated by cPLA2. Ang II (100 nM) increased PLD activity measured as the production of 3H-phosphatidylethanol from phospholipids in VSMC prelabeled with 3H-oleic. Inhibitors of PLD ...
OBJECTIVE: We tested the hypothesis that trophoblast produces 15-hydroxyeicosatetraenoic acid, and its level is elevated in trophoblast from preeclamptic women compared with normal. We also used selective enzymatic inhibitors to determine the relativ
12-Hydroxyeicosatetraenoic acid (12-HETE) is a derivative of the 20 carbon polyunsaturated fatty acid, arachidonic acid, containing a Hydroxyl residue at carbon 12 and a 5Z,8Z,10E,14Z Cis-trans isomerism configuration (Z=cis, E=trans) in its four double bonds. It was first found as a product of arachidonic acid metabolism made by human and bovine platelets through their 12S-lipoxygenase (i.e. ALOX12) enzyme(s). However, the term 12-HETE is ambiquous in that it has been used to indicate not only the initially detected "S" stereoisomer, 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (12(S)-HETE or 12S-HETE), made by platelets, but also the later detected "R" stereoisomer, 12(R)-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (also termed 12(R)-HETE or 12R-HETE) made by other tissues through their ...
The 5-lipoxygenase (5-LO) product 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), which is a potent chemoattractant for myeloid cells, is known to promote the survival of prostate cancer cells. In the present study, we found that PC3 prostate cancer cells and cell lines derived from breast (MCF7) and lung (A-427) cancers contain 5-hydroxyeicosanoid dehydrogenase (5-HEDH) activity and have the ability to synthesize 5-oxo-ETE from its precursor 5S-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) when added as an exogenous substrate. H2O2 strongly stimulated the synthesis of 5-oxo-ETE and induced dramatic increases in the levels of both glutathione disulfide and NADP+. The effects of H2O2 on 5-oxo-ETE and NADP+ were blocked by N-ethylmaleimide (NEM), indicating that this effect was mediated by the glutathione reductase-dependent generation of NADP+, the cofactor required by 5-HEDH. 5-Oxo-ETE synthesis was ...
The current study provides strong evidence for a mechanistic link between the vascular CYP4A-20-HETE pathway and endothelial dysfunction; it clearly demonstrates that 20-HETE impairs NO production in vitro and its function in vivo by uncoupling the eNOS activation process. These findings are novel in that they implicate 20-HETE, a major eicosanoid of the microcirculation, as a key regulator of eNOS and, thereby, endothelial function.. 20-HETE has been recognized for its presence in the vasculature, its role in the regulation of vascular tone, and its contributions to mitogenic and angiogenic responses. In the renal and cerebral microcirculation, 20-HETE elicits vasoconstriction via protein kinase C (PKC)- or tyrosine kinase-sensitive inhibition of the Ca2+-activated K channel, vascular smooth muscle cell depolarization, and elevation in cystolic Ca2+ concentration (21). In small porcine coronary arteries, 20-HETE induces contraction by a mechanism involving the activation of the Rho-kinase ...
PRIMARY OBJECTIVES:. I. To determine mean percentage change from baseline in prostaglandin E2 (PGE2) within ACF pre and post 30 days of curcumin administration at a specified dose.. SECONDARY OBJECTIVS:. I. To determine mean percentage change from baseline in 5-hydroxy-eicosatetraenoic acid (5-HETE) within ACF pre and post 30 days of curcumin administration at a specified dose.. II. To determine mean percentage change from baseline in PGE2 and 5-HETE within comparison normal mucosa pre and post 30 days of curcumin administration at a specified dose.. III. To quantify corresponding enzyme changes in the cyclooxygenases (COX-1, COX-2,) and lipoxygenase (5-LOX) protein abundance. Semi-quantitative changes in these proteins will be measured by western blotting and correlated with changes in prostaglandins and leukotrienes respectively.. IV. Document changes in total ACF number. V. Determine proliferation by Ki-67 IHC in rectal mucosa pre and post therapy and ...
In the present study, we have provided evidence that although CYP4A11 functions as a 20-HETE synthase, CYP4A22 does not contribute to renal 20-HETE biosynthesis. Furthermore, to determine the relevance of genetic variation in CYP4A11 to hypertension, we identified a coding polymorphism (T8590C), biochemically characterized 8590C as a loss-of-function variant, and demonstrated its association with hypertension in whites. Initial findings of associations between biologically plausible functional variants are often not replicated in follow-up studies, and even when associations are replicated, they are often less strong than in the initial validation cohort.27 For this reason, we conducted a study of replication in a population-based cohort of whites. Our results suggest the existence of a modest association finding in the Framingham cohort. Clearly, our findings strongly support further study of the relationship of variants in this gene with hypertension in other populations.. Both animal14,16 and ...
Leukotriene (LT) A4 hydrolase (EC 3.3.2.6) is a bifunctional zinc metalloenzyme that catalyzes the hydrolysis of the unstable epoxide intermediate LTA4 into the proinflammatory substance LTB4 and also exhibits an amidase/peptidase activity toward synthetic substrates. Based on proposed reaction mechanisms for other zinc hydrolases, we have synthesized inhibitors of LTA4 hydrolase and evaluated their effects on the formation of LTB4 from LTA4 using both purified enzyme and intact polymorphonuclear leukocytes. The two most effective inhibitors, an alpha-keto-beta-amino ester (compound IV) and a thioamine (compound VIII), exhibited IC50 values of 1.9 +/- 0.9 and 0.19 +/- 0.12 microM (mean +/- SD, n = 4), respectively. Compounds IV and VIII were also potent inhibitors of LTB4 biosynthesis in ionophore stimulated polymorphonuclear leukocytes with IC50 , 200 nM. At higher concentrations, the biosynthesis of 5-hydroxy-eicosatetraenoic acid was also inhibited with ...
The present study examined whether preglomerular arterioles of the rat produce 20-hydroxyeicosatetraenoic acid (20-HETE) and whether 20-HETE is vasoactive on these vessels. Raf preglomerular arterioles produced 20-HETE (4.8 +/- 1.0 pmol.min-1.mg-1, n = 7) and, to a lesser extent, 14-, 15-, 11-, and 12-dihydroxyeicosatetraenoic acid, 6-ketoprostaglandin F/alpha and prostaglandin E2 when incubated with [14C]larachidonic acid. The results of immunoblotting and reverse-transcription polymerase chain reaction experiments indicate that these vessels express mRNA and protein for a P-450 4A2 enzyme. With the use of a rat juxtamedullary nephron microvascular preparation perfused in vitro with a cell-free media, addition of 20-HETE (1 nM-1 microM) to the bath reduced the diameter of proximal and distal portions of the efferent arterioles. At a concentration of 1 microM, the diameter of the proximal ...
The present study evaluated the contribution of cGMP-dependent versus cGMP-independent pathways in mediating the effects NO on K+ channel activity and vascular tone in renal arterioles. The results indicate that NO selectively enhances the NPo of a large-conductance (195±9 pS) K+ channel in renal VSM cells in a concentration-dependent manner. NO had no significant effect on the activity of the two smaller conductance channels in renal VSM cells that were previously identified as a small-conductance, apamin-sensitive, KCa channel33 and the 4-aminopyridine-sensitive delayed rectifier channel.28 34 35 36 The K+ channel activated by NO is voltage-sensitive and is blocked by TEA and iberiotoxin which are selective inhibitors of the KCa channel.37 38 39 Thus, the present findings indicate that NO activates KCa channels in renal VSM cells, and they are consistent with the results of recent studies with smooth muscle cells isolated from other tissues.6 7 9 11 13 15 Additional experiments were performed ...
1. Since ageing has been associated with a decrease in both immune responses and antioxidant defences, this study was undertaken to compare the glutathione peroxidase activity in peripheral blood mononuclear cells of healthy elderly and young donors. The mean value of glutathione peroxidase activity was significantly lower in the aged group (−36%) than that observed in the young control group (n = 10).. 2. This defect was accompanied by a marked increase (+106%) in the oxygenated metabolism of endogenous arachidonic acid by lipoxygenases as judged by the radiolabel associated with hydroxyeicosatetraenoic acids in [3H]arachidonic acid-prelabelled peripheral blood mononuclear cells, whereas the cyclo-oxygenase activity, estimated by the radiolabel associated with thromboxane B2, was not significantly altered.. 3. Upon stimulation by the mitogenic lectin concanavalin A, the radioactivity associated with total eicosanoids (free ...
The eicosanoids are a group of biologically active fatty acids derived from C20 eicosanoic precursors. The eicosanoid generating capabilities of calcium ionophore stimulated rainbow trout peripheral blood cells and solid tissue were examined. Adherent leucocytes (monocytes/granulocytes) generated a plethora of eicosanoids including prostaglandins (PG), monohydroxy eicosatetraenoic acids (HETEs), monohydroxy eicosapentaenoic acids (HEPEs), lipoxins (LX), leukotrienes (LT) and other diHETEs/diHEPEs. Thrombocytes displayed a similar eicosanoid profile, and in addition, were also prolific generators of thromboxanes. Purified lymphocytes generated only trace amounts of lipoxygenase products suggesting that these cells are either poor generators or that they lack lipoxygenase activity. Trout erythrocytes generated no detectable amounts of cyclo-oxygenase or lipoxygenase products. All the solid tissues generated ...
Neither moxifloxacin nor cefuroxime produces significant attenuation of inflammatory mediator release in patients exposed to cardiopulmonary bypass: a randomized controlled trial.
11S,12S)-Epoxy-5,14-cis-7,9-trans-eicosatetraenoic acid (11,12-leukotriene A4) was nonenzymically converted to seven compounds: two diastereomers of (12S)-hydroxyeicosatetraeno-delta-lactones (major products), two diastereomers of (5,12S)-dihydroxyeicosatetraenoic acid and three stereoisomers of (11,12S)-dihydroxyeicosatetraenoic acid. Among these compounds, (11R,12S)-dihydroxy-5,14-cis-7,9-trans-eicosatetraenoic acid proved to be the only enzymic product. This hydrolysis activity was present in the cytosol fractions of various ...
20-HETE is pro-proliferative, -inflammatory, and -migratory (49, 66), all of which contribute to the pathogenesis of systemic and pulmonary hypertension. Although the increase in 20-HETE by hypoxia inhibited acute hypoxia-induced pulmonary artery constriction (2, 69), it time and dose dependently increased superoxide production from NADPH oxidases in the cultured pulmonary artery endothelial cells (37). Also, studies have reported that 20-HETE-induced superoxide mediated flow-induced constriction of cerebral arteries (58). Our current results further demonstrated that inhibition of 20-HETE biosynthesis by DDMS decreased mitochondrial superoxide generation and conversely application of 20-HETE to endothelium denuded pulmonary arteries for 12 h elicited superoxide generation from mitochondria but not from extra-mitochondrial sources. This led us to the question of how does 20-HETE increase mitochondrial superoxide generation? One potential explanation was 20-HETE passed through the gap junctions ...
Pulmonary arterial hypertension (PAH) is a disease characterized by thickening of pulmonary artery walls, elevated pulmonary vascular resistance, pulmonary vascular thrombotic lesions and right-heart failure. Recent studies suggest that 15-lipoxygenase (15-LO) /15-hydroxyeicosatetraenoic acid (15-HETE) play an important role in PAH, acting on arterial walls. Here, we show evidence for the action of the 15-LO/15-HETE signaling in the pulmonary vascular thrombotic lesions in the experimental PAH models. Platelet deposition was augmented in hypoxia+su5415 rats, which were both prevented by NDGA, a 15-LO inhibitor. Chronic hypoxic resulted in the platelet deposition specifically in pulmonary vasculature, which was reversed by 15-LO inhibitor. 15-LO pathway mediated in the endothelial dysfunction induced by hypoxia in vivo. Meanwhile, 15-HETE positively regulated the generation of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1). The ...
Two types of 12-lipoxygenase that catalyze the transformation of arachidonic acid to 12(S)-hydroperoxyeicosatetraenoic acid (12-HPETE) have been previously classified into platelet-type and leukocyte-type categories. Here, we document, for the first time, a molecular characterization of both forms within the same species. The amino acid sequence of the murine platelet 12-lipoxygenase deduced from its cDNA is 58% identical to the murine spleen/leukocyte 12-lipoxygenase. Expression constructs carrying the cDNAs for the two 12-lipoxygenase forms were introduced into human embryonic kidney 293 cells. The platelet-type enzyme metabolized arachidonic acid exclusively to 12-HPETE, whereas the leukocyte-type enzyme formed both 12-HPETE and 15-hydro(pero)xyeicosatetraenoic acid in a ratio of approximately 3:1. Linoleic acid was metabolized to a similar extent by the latter enzyme to ...
Endothelium-derived metabolites of AA modulate vascular tone and mediate the vascular effects of shear stress and hormones such as bradykinin, acetylcholine, substance P and others (2, 4, 19). CYP450 epoxygenase and lipoxygenase metabolites of AA relax arteries. While COX metabolites such as PGI2 relax arteries, other COX metabolites act as endothelium-derived constrictor factors. The effects of these AA metabolites and their mechanisms of action vary with artery and species.. 12-HETE is the primary LO-derived AA metabolite produced by mouse aorta, carotid, femoral, mesenteric arteries (10), and cerebral microvessels (20, 21). Thus we continued to investigate the function and chiral properties of 12-HETE in these arteries. To establish the enzymatic source of 12-HETE, we determined its stereoisomer configuration. While 12(S)-HETE is a product of the 12(S)-lipoxygenase pathway, 12(R)-HETE is produced by either 12(R)-lipoxygenase or CYP450 (5, 17). HPLC chiral analysis of the mouse vascular ...
Formation of the 12R-lipoxygenase product, 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE), has been detected previously only in human skin (Boeglin et al. (1998) Proc. Natl. Acad. Sci. USA 95, 6744). The unexpected appearance of an EST sequence (AA649213) for human 12R-lipoxygenase from germinal center B lymphocytes purified from human tonsils prompted our search for the existence of the enzyme in this novel source. Incubation of [1-14C]arachidonic acid with homogenates of human tonsillar tissue yielded mixtures of radiolabeled 12-HETE and 15-HETE. Stereochemical analysis showed varying ratios of 12S- and 12R-HETE, while 15-HETE was exclusively of the S-configuration. Using stereospecifically labeled [10S-3H]- and [10R-3H]arachidonic acid substrates we detected pro-R hydrogen abstraction at carbon 10 associated with formation of 12R-HETE. This mechanistic evidence implicates a 12R-lipoxygenase in the biosynthesis of 12R-HETE. The mRNA for ...
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY FOR THE STUDY OF ARACHIDONIC ACID METABOLITES Xiaojing Liu Ian A. Blair, PhD Arachidonic acid (AA) oxidation metabolism has been an important research topic for decades, and numerous oxidation products as well as enzymes involved in AA metabolism together with their downstream metabolites have been identified, although unknown pathways still remain to be characterized. In the present a study a new AA metabolite, 11-oxo-eicosatetraenoic acid (ETE), generated from a major product of cyclooxygenase (COX-2), 11(R)-hydroxyeicosatetraenoic acid (HETE), through 15-hydroxyprostaglandine dehydrogenase (15-PGDH)-mediated oxidation. 11-Oxo-ETE was found to have an anti-proliferative effect on human umbilical vein endothelial cells (HUVECs), with a similar IC50 to a well- known anti-inflammatory mediator, 15-deoxy-12,14-prostaglandin J2 ...
TY - JOUR. T1 - Cyclooxygenase-2-mediated metabolism of arachidonic acid to 15-oxo-eicosatetraenoic acid by rat intestinal epithelial cells. AU - Seon, Hwa Lee. AU - Rangiah, Kannan. AU - Williams, Michelle V.. AU - Wehr, Angela Y.. AU - DuBois, Raymond N.. AU - Blair, Ian A.. PY - 2007/11. Y1 - 2007/11. N2 - Rat intestinal epithelial cells mat permanently express the cyclooxygenase-2 (COX-2) gene (RIES cells) were used to investigate COX-2-mediated arachidonic acid (AA) metabolism. A targeted chiral lipidomics approach was employed to quantify AA metabolites that were secreted by the cells into the culture media. When intact RIES cells were treated with calcium ionophore A-23187 (1 μM) for 1 h, 11-(R)-hydroxyeicosatetraenoic acid (HETE) was the most abundant metabolite, followed by prostaglandin (PG) E2, 15-(S)-HETE, 15-oxo-eicosatetraenoic acid (ETE), and ...
Previous studies demonstrated a requirement for arachidonic acid metabolites in tumor development in mouse skin. The goal of this study was to determine whether the arachidonate content of epidermal phospholipids could be altered by increasing dietary levels of linoleate and whether specific metabolites of linoleate and arachidonate have dissimilar biological effects. In a series of tumor studies in which the quantity of dietary linoleate was incrementally increased, a slight reduction in phospholipid levels of arachidonate was observed that correlated with an increased phospholipid level of linoleate and a suppression in tumor yield. A comparison of the arachidonate lipoxygenase metabolite 12-hydroxyeicosatetraenoic acid (12-HETE) with the 13-hydroxyoctadecadienoic acid (13-HODE) lipoxygenase metabolite of linoleate revealed that 12-HETE has biological activities that mimic the phorbol ester tumor ...
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Convincing evidence exists implicating leukocyte-type 12/15-LO as a key player in the development of atherosclerosis and restenosis. KO of the 12/15-LO gene in apolipoprotein E- and LDL receptor-deficient mice, 2 models of robust atherosclerosis, resulted in a marked decrease in atherosclerosis development in vivo.5,7 Marked upregulation of 12/15-LO occurred in VSMCs after balloon carotid injury in rats, and restenosis was significantly blocked with a pharmacological 12/15-LO inhibitor as well as a specific ribozyme targeted to cleave 12/15-LO.4,27 Many events and pathways involving 12/15-LO have been proposed to contribute to atherosclerosis and restenosis. Much is understood concerning the ability of 12/15-LO within macrophages to participate in the oxidation of LDL, an early event in vascular lesion formation.2,7-9,28 However, 12/15-LO activity and products also increase in endothelial cells and VSMCs at the time of injury and in response to growth factors and cytokines. 12S-HETE, the ...
HgCl2 induced a 2-fold increase in β-hexosaminidase release, and also significant VEGF release at 0.1 and 1 μM (311 ± 32 pg/106 cells and 443 ± 143 pg/106 cells, respectively) from LAD2 mast cells compared to control cells (227 ± 17 pg/106 cells, n = 5, p , 0.05). Addition of HgCl2 (0.1 μM) to the proinflammatory neuropeptide substance P (SP, 0.1 μM) had synergestic action in inducing VEGF from LAD2 mast cells. HgCl2 also stimulated significant VEGF release (360 ± 100 pg/106 cells at 1 μM, n = 5, p , 0.05) from hCBMCs compared to control cells (182 ± 57 pg/106 cells), and IL-6 release (466 ± 57 pg/106 cells at 0.1 μM) compared to untreated cells (13 ± 25 pg/106 cells, n = 5, p , 0.05). Addition of HgCl2 (0.1 μM) to SP (5 μM) further increased IL-6 release ...
Oxoeicosanoid receptor 1 (OXER1) also known as G-protein coupled receptor 170 (GPR170) is a protein that in humans is encoded by the OXER1 gene located on human chromosome 2p21; it is the principle receptor for the 5-Hydroxyicosatetraenoic acid family of carboxy fatty acid metabolites derived from arachidonic acid. The receptor has also been termed hGPCR48, HGPCR48, and R527 but OXER1 is now its preferred designation. OXER1 is a G protein-coupled receptor (GPCR) that is structurally related to the hydroxy-carboxylic acid (HCA) family of G protein-coupled receptors whose three members are HCA1 (GPR81), HCA2 (Niacin receptor 1), and HCA3 (Niacin receptor 2); OXER1 has 30.3%, 30.7%, and 30.7% amino acid sequence identity with these GPCRs, respectively. It is also related (30.4% amino acid sequence identity) to the recently defined receptor, GPR31, for the ...
Downloadable! This paper examines four different daily datasets of hedge fund return indexes: MSCI, FTSE, Dow Jones and HFRX, all based on investable hedge funds, and three different monthly datasets of hedge fund return indexes: CSFB, CISDM and HFR which comprise both investable and non-investable hedge funds. Our study, based on standard statistical analysis, non-parametric analysis of the distribution and non-parametric regressions with respect to the S&P500 index shows that key data biases and disparate index construction methodologies lead to different statistical properties of hedge fund databases. One key variable that highly affects the statistical properties of hedge fund index returns is the �investability� of hedge funds
November 4, 1999. Hedge Balls, Acorns and Hardware. Grazing short pastures, which most beef producers are doing, provides the opportunity for beef cattle to consume numerous particles that oftentimes create problems producers need to be aware of. The height of the grass available for grazing certainly leaves something to be desired. One prominent cattle producer reminded me several years ago to remember that under short grazing conditions an old cow will eat anything. Whenever we have these grazing conditions the door is open for obstacles we normally don t think about.. One of these problems is the availability of hedge apples or hedge balls. I know of no digestive problems resulting from cattle consuming hedge balls. However, choking is a problem if large portions of the hedge ball are swallowed or the hedge ball is swallowed whole. Cows choking on hedge balls have excessive salivation, and bloat frequently occurs resulting in death due to not being able to belch. Lodging of the large ...
BioAssay record AID 3623 submitted by ChEMBL: Inhibition of [14C]arachidonic acid conversion to 5-HETE by broken cell 5-LO isolated from guinea pig PMN.
Purified human monocytes release and metabolize endogenous arachidonic acid (20:4) from phospholipid stores when challenged with particulate inflammatory stimuli or the calcium ionophore A23187. Using radiolabeled cultures, the percentage of total [3H]20:4 released was similar with each type of stimulus. However, the spectrum of 20:4 metabolites differed. With opsonized zymosan (OpZ) or Sephadex beads coated with IgG immune complexes (Ig-beads), the predominant product was thromboxane (25% of the total) together with smaller amounts of other cyclo-oxygenase products and lipoxygenase metabolites. Levels of thromboxane synthesis by monocytes were comparable to those by platelets, as measured by radioimmunoassay. In contrast, exposure to the nonspecific agent A23187 led to mainly lipoxygenase products (70% of the total). Monocytes isolated from mononuclear cell fractions of peripheral blood contain platelets specifically rosetted to their surfaces. These platelet contaminants were removed by ...
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Downloadable (with restrictions)! This paper investigates the variance minimizing currency forward hedge of an exporting firm that is exposed to different sources of risk. In an empirical study, we quantify the corresponding hedge ratios of a typical German firm for different hedge horizons. Based on cointegrated vector autoregressive models of prices, interest rates and exchange rates, we show that hedge ratios decrease substantially with the hedge horizon for different currencies, reaching values of one half or less for a ten-years horizon. Our findings can partly explain underhedging of long-term exchange rate exposures and have important implications for the design of risk management strategies.
Leukocyte migaration from the blood into tissues is vital for immune surveillance and inflammation. During this diapedesis of leukocytes, the leukocytes bind to endothelial cell adhesion molecules (CAM) and then migrate across the vascular endothelium. A leukocyte adherent to CAMs on the endothelial cells moves forward by leading-edge protrusion and retraction of its tail. In this process, alphaL /beta2 integrin activates through Vav1, RhoA, which subsequently activates the kinase p160ROCK. ROCK activation leads to MLC phosphorylation, resulting in retraction of the actin cytoskeleton. Moreover, Leukocytes activate endothelial cell signals that stimulate endothelial cell retraction during localized dissociation of the endothelial cell junctions. ICAM-1-mediated signals activate an endothelial cell calcium flux and PKC, which are required for ICAM-1 dependent leukocyte migration. VCAM-1 is involved in the opening of the "endothelial passage" through which leukocytes can extravasate. In this ...
Chemicals. Retinol, retinal, retinoic acid, and arachidonic acid were purchased from Sigma-Aldrich (St. Louis. MO). 14C-labeled substrates were purchased from PerkinElmer Life and Analytical Sciences (Boston, MA), and 3H-DMBA was obtained from Amersham Biosciences Inc. (Piscataway, NJ). Complete Protease Inhibitor Tablets were obtained from Roche Diagnostics (Indianapolis, IN). The QuikChange Site-Directed Mutagenesis Kit was obtained from Stratagene (La Jolla, CA). Human CYP1A1 and CYP1B1 Supersomes were obtained from BD Gentest (Woburn, MA). Arachidonate metabolite standards 20-HETE, 15-HETE, 11-HETE, 9-HETE, 5-HETE, 8-HETE, and 12-HETE; and 14,15-EET, 11,12-EET, 8,9-EET, and 5,6-EET were obtained from Cayman Chemical (Ann Arbor, MI).. Expression and Purification of CYP1B1 and Cyp1b1. Human CYP1B1 was expressed and purified as described earlier (Jansson et al., 2001). The mouse Cyp1b1 transcript was prepared from a cDNA library using primers with modification at the 5′ end ...
Like mutual funds, hedge funds pool investors money and invest those funds in financial instruments in an effort to make a positive return. Many hedge funds seek to profit in all kinds of markets by pursuing leveraging and other speculative investment practices that may increase the risk of investment loss.. Unlike mutual funds, however, hedge funds are not required to register with the SEC. Hedge funds typically issue securities in "private offerings" that are not registered with the SEC under the Securities Act of 1933. In addition, hedge funds are not required to make periodic reports under the Securities Exchange Act of 1934. But hedge funds are subject to the same prohibitions against fraud as are other market participants, and their managers have the same fiduciary duties as other investment advisers.. What are "funds of hedge funds?". A fund of hedge funds is an investment company that invests in hedge funds -- rather than investing in individual securities. Some funds of hedge funds ...
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A polymer having a molecular weight between 1,000 and 1,500,000 characterized in that it contains the radical of a polyunsaturated acid selected from the group consisting of 8,11,14-eicosatrienic acid, 5,8,11,14-eicosatetraenoic acid, and 5,8,11,14,17-eicosapentenoic acid, bound to a macromolecular backbone of polyacrylic or polymethacrylic units through oxyalkylenic, aminoalkylenic or oxyaminoalkylenic chains.
Abstract: : Purpose: MUC1 mucin is prominently expressed at the ocular surface, both as a transmembrane protein on conjunctival and corneal epithelial cells and as a secreted glycoprotein in aqueous tears. The eicosanoid 15(S)-HETE has been shown to stimulate MUC1 secretion from human conjunctiva and to thicken the mucin layer that coats the rabbit corneal surface, although the underlying biochemical mechanisms are unknown. The present study was undertaken to evaluate the role of Ca(2+)in 15(S)-HETE-induced MUC1 secretion by CEPI cells, an SV40 virus-immortalized human corneal epithelial cell line. Methods: Expression of MUC1 mRNA and protein by CEPI cells was demonstrated by RT-PCR and Western blot analysis, respectively. Secretion of MUC1 was measured by dot blot immunoassay using DF3 anti-MUC1 antibody and purified Ca15-3 antigen as the standard. In general, cells were pre-incubated with pharmacological agents for 30 min followed by a 60 min incubation in medium ± 15(S)-HETE. Results: ...
Ebola virus (EBOV), a member of the family Filoviridae, causes an acute hemorrhagic fever with approximately 90% fatality within seven to eleven days in those showing symptoms. Fatal viral infection overwhelms the host immune system, leading to massive inflammatory mediator release in conjunction with T and B cell apoptosis. Survival and asymptomatic disease are possible following…
It is the smart notes on How Eicosanoids are synthesizing in Human body, This notes explained with suitable images. Eicosanoids synthesis is very effective.
La arachidonato 12-lipossigenasi è un enzima appartenente alla classe delle ossidoreduttasi, che catalizza la seguente reazione: arachidonato + O2 ⇄ (5Z,8Z,10E,14Z)-(12S)-12-idroperossiicosa-5,8,10,14-tetraenoato Il prodotto è rapidamente ridotto nel corrispondente composto 12S-idrossi. Hamberg, M. and Samuelsson, B., Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets, in Proc. Natl Acad. Sci. USA, vol. 71, 1974, pp. 3400-3404, Entrez PubMed 4215079. Nugteren, D.H., Arachidonate lipoxygenase in blood platelets, in Biochim. Biophys. Acta, vol. 380, 1975, pp. 299-307, Entrez PubMed 804329. Wallach, D.P. and Brown, V.R., A novel preparation of human platelet lipoxygenase. Characteristics and inhibition by a variety of phenyl hydrazones and comparisons with other lipoxygenases, in Biochim. Biophys. Acta, vol. 663, 1981, pp. 361-372, Entrez PubMed 6783111 ...
To a stirred solution of p-toluenesulfonhydrazide (0.61g, 3.3 mmol) in methanol/water (6:4, 20 ml) at 60°C was added 2-cinnamyl-1,3-oxazole-4-carboxaldehyde [1](0.50 g, 2.5 mmol).[...]
Ron Insana has rejoined CNBC, just days after abandoning his effort to become a hedge fund mogul.. Insana was a primary anchor on the business cable channel until 2006, when he quit to set up his own fund of hedge funds. He shuttered Insana Capital Partners in August, and then joined hedge fund giant SAC Capital Management as a managing partner.. Now, hes back on television as a senior analyst for CNBC. The newsman-cum-hedgie-cum-newsman returned to the small screen today, on the Power Lunch program.. ...
|p|The Black and Decker GT5055 is an electric powered Hedge Trimmer with a 55cm blade length. The 22mm blade gap is suitable for medium hedges. |/p||p|The Hedge Trimmer has a durable, robust and sharp metallic blade with an angled blade design for optimum
With a neglected or overgrown hedge, renewal pruning can be effective. Renewal pruning should be done in late winter or early spring before it leafs out for the year. To renew, cut it down very short, close to the ground. The new growth is then trimmed often, every time it grows about 6 inches it is sheared or trimmed off again. The more often it is cut, the better. This forces lots of branching at the base of the hedge. Eventually as it grows taller it is trained at an angle -- narrow top, wide base -- to keep it leafy at the bottom. (The hedge grows back quite fast because it already has a big, established root system ...
The kidney receive 20-25 percent of the total cardiac output. In normal healthy individuals, about 1200ml of blood flows through the kidneys each minute. The blood supply to each kidney is usually a single renal artery originates along the lateral surface of the adominal aorta near the level of the superior mesenteric artery. However, there may sometimes be additional small vessels from superior mesenteric, adrenal, spermatic or ovarian arteries.. Almost all of the blood which enters the kidneys at renal hilus, via the renal artery. The renal artery branches to form several interlobar arteries which radiate outward through the renal column between the renal pyramids. The interlobar arteries subdivide themselves to arcuate arteries, which pass along the boundary between cortex and medulla. Each arcuate artery branches and travel out at a right angle, through the cortex towards the capsule and gives rise to a number of interlobular arteries, which supply the cortical portions of the adjacent renal ...
Vonach C, Viola K, Giessrigl B, Huttary N, Raab I, Kalt R, Krieger S, Vo TP, Madlener S, Bauer S, Marian B, Hämmerle M, Kretschy N, Teichmann M, Hantusch B, Stary S, Unger C, Seelinger M, Eger A, Mader R, Jäger W, Schmidt W, Grusch M, Dolznig H, Mikulits W, Krupitza G. NF-κB mediates the 12(S)-HETE-induced endothelial to mesenchymal transition of lymphendothelial cells during the intravasation of breast carcinoma cells. Br. J Cancer. 105:263-71 (2011).. ...
The right interlobar artery is not visible, because it is not surrounded by aerated lung but by the collapsed lower lobe, which is adjacent to the right atrium.. On a follow-up chest film the atelectasis has resolved. We assume that the atelectasis was a result of post-traumatic poor ventilation with mucus plugging.. Notice the reappearance of the right interlobar artery (red arrow) and the normal right heart border (blue arrow).. ...
A recognized feature of psoriasis and other proliferative dermatoses is accumulation in your skin from the unusual arachidonic acidity metabolite, 12hydrogen through the 10-carbon of arachidonate. included regions of epidermis in psoriasis possess PPARG improved concentrations of free of charge arachidonic acid and 12-HETE markedly. Chiral analysis from the 12-HETE in psoriasis revealed that the major enantiomer is 12390C404, encompassing the major M-PFB ions at 391 (unlabeled HETE) and 399 (d8 analogue), essentially as described previously (28). Experiments with Stereospecifically Labeled Arachidonic Acids. The specific activities of the two 10-3H-labeled arachidonic acids were approximately 10,000C20,000 disintegrations per min 3H per g. The pro-[10-3H]arachidonic acid was enriched in tritium by incubation with an 8as described in principle before (29). The stereospecifically labeled arachidonic acids were ...
Rigaud, M; Durand, J; and Breton, J C., "Transformation of arachidonic acid into 12-hydroxy-5,8,10,14- -eicosatetraenoic acid by mouse peritoneal macrophages." (1979). Subject Strain Bibliography 1979. 410 ...
BACKGROUND AND PURPOSE: Arachidonic acid that is released following cerebral ischemia can be metabolized to 20-hydroxyeicosatetraenoic acid (20-HETE). 20-HETE is a potent vasoconstrictor that may contribute to ischemic injury. This study examined the effects of blockading the synthesis of 20-HETE with TS-011 on infarct size after transient occlusion of the ...
Astrocytes perform critical non-cell autonomous roles following CNS injury that involve either neurotoxic or neuroprotective effects. Yet the nature of potential prosurvival cues has remained unclear. In the current study, we utilized the close interaction between astrocytes and retinal ganglion cells (RGCs) in the eye to characterize a secreted neuroprotective signal present in retinal astrocyte conditioned medium (ACM). Rather than a conventional peptide neurotrophic factor, we identified a prominent lipid component of the neuroprotective signal through metabolomics screening. The lipoxins LXA4 and LXB4 are small lipid mediators that act locally to dampen inflammation, but they have not been linked directly to neuronal actions. Here, we determined that LXA4 and LXB4 are synthesized in the inner retina, but their levels are reduced following injury. Injection of either lipoxin was sufficient for neuroprotection following acute injury, while inhibition of key lipoxin pathway components ...
Astrocytes perform critical non-cell autonomous roles following CNS injury that involve either neurotoxic or neuroprotective effects. Yet the nature of potential prosurvival cues has remained unclear. In the current study, we utilized the close interaction between astrocytes and retinal ganglion cells (RGCs) in the eye to characterize a secreted neuroprotective signal present in retinal astrocyte conditioned medium (ACM). Rather than a conventional peptide neurotrophic factor, we identified a prominent lipid component of the neuroprotective signal through metabolomics screening. The lipoxins LXA4 and LXB4 are small lipid mediators that act locally to dampen inflammation, but they have not been linked directly to neuronal actions. Here, we determined that LXA4 and LXB4 are synthesized in the inner retina, but their levels are reduced following injury. Injection of either lipoxin was sufficient for neuroprotection following acute injury, while inhibition of key lipoxin pathway components ...
Purpose: Colorectal Cancer (CRC) is the third most prevalent cancer with over 148,000 cases and almost 50,000 deaths estimated in 2008. Between 60 to 70% of all CRC cases are diagnosed at late stages when 5-year survival rates average less than 10 to 40%. Consequently, prevention of CRC with low toxicity agents could lead to considerable decrease in CRC morbidity and mortality. In vitro studies, murine models and trials in humans using drugs that inhibit cyclooxygenase enzymes (COX1 & COX2) indicate that up-regulation of inflammatory eicasanoids and in particular prostaglandin E2 (PGE2) are early events in the development of CRC. Ginger (Zingiber officinale) and its constituent\#8217;s gingerols and shogaols have demonstrated down regulation of COX1 and COX2, apoptosis in CRC cell lines and decreased incidence and multiplicity of adenomas in rats. We conducted a study to determine if ginger extract standardized to 5% gingerols could decrease the concentration of PGE2, ...
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016 ...
The relationships between arachidonic acid (AA) metabolism and chloride secretion were investigated in mucosal preparations of rabbit distal colon. Tissues displayed a significant cyclooxygenase activity already in nonstimulated conditions and incubation with exogenous AA and calcium ionophore A23187 produced a predominant prostaglandin F2 alpha (PGF2 alpha) profile [PGF2 alpha greater than PGE2 greater than thromboxane B2 (TxB2) greater than 6-keto-PGF1 alpha] as assessed by HPLC of tissue homogenates, whereas 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) was not detected in AA- or A23187-stimulated tissues. Radioimmunological assays showed that PGE2 synthesis was time dependent, plateaued at 10 min, and proceeded at rates 15-20 times over TxB2 and 6-keto-PGF1 alpha. Among the PGs produced by colonic mucosa, only PGE2 and, to a lower extent, PGF2 alpha were found to stimulate chloride secretion and cAMP synthesis. Pretreatment with 10 ...
Global hedge fund assets crossed the $3 trillion (€2.2 trillion) mark in May for the first time on record, according to the May 2014 hedge fund asset flows report from eVestment.
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To ensure that your Thuja hedge stays dense, it is important to take care of it. Thuja hedges can actually be pruned throughout the year - at frost-free temperatures. Nevertheless, January is well suited to cut back the plant, since it reduces its growth in the end of January. In February the plant grows more slowly which affects the healing process in a positive way. Do not choose a too sunny day. Best time is late afternoon. This way, the freshly cut wounds do not get burned and dry well ...
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The healthy corneal epithelium has tight junctions and forms a barrier between the environment and the inside of the eye. The tear film aids in protecting the corneal surface, and artificial tear...
The presence of specific leukotriene B4 (LTB4) binding sites was investigated in membranes prepared from mouse, rat, guinea pig and rabbit brain. Specific binding sites have been found in guinea pig brain membranes (GPBM) but not in the other species studied. Specific binding of LTB4 to GPBM was proportional to membrane concentration, saturable and reversible, reaching a steady-state suggesting equilibrium after 30 min of incubation. Analysis of the binding data showed a single binding site with a Kd = 2.27 +/- 0.55 nM and a Bmax = 576 +/- 89.6 fmol/mg protein. The relative potencies of LTB4 analogs to displace the tritiated ligand were LTB4 greater than 20-hydroxy-LTB4 much greater than 20-carboxy-LTB4 greater than 12-(S)hydroxy-5,8,10,14(Z,Z,E,Z)-eicosatetraenoic acid; LTD4 and LTC4 did not displace the ligand in concentrations up to 50 microM. The binding was inhibited by monovalent cations and GTP[gamma S] and increased by divalent ...
Nichols, Frank C., "Arachidonic Acid Metabolism in ros 17/28 Cells Treated with Parathyroid Hormone and in Human peripheal Blood Monocytes Treated with the complement Fragment, C3b and Bacterial Lipopolysaccharide" (1984). SoDM Masters Theses. 96 ...
From one of our customers: Hi Ronnie! Just an email to say that we love the Sashas Blend, (all but the smell, LOL) it has made a huge improvement in our dogs walking, and we will order more as needed. Thanks so much! Teryl SASHAS BLEND Australias # 1 selling joint supplement. Fast acting whole food, blend of Marine concentrates. New Zealand Green Lipped Mussel - A natural anti-inflammatory Abalone Joint Lubricator - rebuilds synovial fluid Marine Cartilage - Provides components (GAGS) to rebuild cartilage A Unique Combination of Marine Concentrates for Dogs and Cats in a vanilla and honey flavoured powder 1. New Zealand Green Lipped Mussel (Perna canaliculus): - contains high levels of glycosaminoglycans, as well as antioxidants and omega-3 fatty acids. A unique omega-3 called eicosatetraenoic acid (ETA) is believed to have a potent anti-inflammatory activity. Recent studies in dogs with arthritis have shown that Green Lipped Mussel can be of significant ...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
0065] In one embodiment, the pharmaceutical composition comprises a stabilizer such as a cetyl alcohol or a saturated cetyl alcohol (e.g., cetyl alcohol). In one embodiment, the pharmaceutical composition comprises about 0.1 wt. % to about 5 wt. % of a stabilizer, for example about 0.1 wt. %, about 0.11 wt. %, about 0.12 wt. %, about 0.13 wt. %, about 0.14 wt. %, about 0.15 wt. %, about 0.16 wt. %, about 0.17 wt. %, about 0.18 wt. %, about 0.19 wt. %, about 0.2 wt. %, about 0.21 wt. %, about 0.22 wt. %, about 0.23 wt. %, about 0.24 wt. %, about 0.25 wt. %, about 0.26 wt. %, about 0.27 wt. %, about 0.28 wt. %, about 0.29 wt. %, about 0.3 wt. %, about 0.31 wt. %, about 0.32 wt. %, about 0.33 wt. %, about 0.34 wt. %, about 0.35 wt. %, about 0.36 wt. %, about 0.37 wt. %, about 0.38 wt. %, about 0.39 wt. %, about 0.4 wt. %, about 0.41 wt. %, about 0.42 wt. %, about 0.43 wt. %, about 0.44 wt. %, about 0.45 wt. %, about 0.46 wt. %, about 0.47 wt. %, about 0.48 wt. %, about 0.49 wt. %, about 0.5 wt. %, ...
We want to see GM emerge as a stronger and viable manufacturer, providing thousands of jobs and products that appeal to consumers around the world. While we have not seen the revised business plan being developed by the Company and the Auto Task Force (ATF), we commit to working constructively with all parties on a plan that sets GM on the right path forward towards a financially healthy, operationally sound competitor. We will present our ideas to the ATF this afternoon and look forward to an ongoing dialogue and an agreement that is fair and equitable to all parties. Time is of the essence, and we stand ready to engage with all the parties to get to a solution that works," Siegert said ...
Fast-growing, dense bamboo hedge grows almost anywhere in zones 7-11. The Bambusa Glaucescens Multiplex quickly creates a thick, manageable green wall.
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Chris Hedges articles and opinion pieces published by Common Dreams, a non-profit independent media outlet publishing since 1997 in Portland, Maine. Page 7
Kúra 3hetes blitz-ekből hét DIANABOL (Naposim) 5mg/tab SUSTANON 250mg/amp WINSTROL 2 mg/tab PRIMOBOLAN 100mg/amp DECA-DURABOLIN 200mg/amp HCG 5000I.
Deschamps, J. D., Gautschi, J. T., Whitman, S., Johnson, T. A., Gassner, N. C., Crews, P., & Holman, T. R. (2007). Discovery of platelet-type 12-human lipoxygenase selective inhibitors by high-throughput screening of structurally diverse libraries. Bioorg ...
News about scandalous stem cell paper retraction from Nature journal blew up in the mass media last week. This event reminded me about some other
Similar bands:. Figure 1 C RT-PCR band - second from bottom (label is obscured by RETRACTED lettering, but "RNA" is visible under the first "E" of RETRACTED).. Page 84 Figure 5? Figure legend label is hidden under RETRACTED lettering. Figure 5 A second last band "DHFR".. These two RT-PCR bands are the same image, slightly darker in Fig 1 than in Fig 5, but the lighter smudges in the middle of the bands in the fourth and fifth lanes (counting from the left) are too identical for comfort.. Another example:. Fig 1 C top two bands labeled 5S rRNA and tRNATyr are the same image, slightly stretched in the 5S rRNA band relative to the tRNATyr band.. and another:. Fig 1 B (immunoblot) bottom two lanes TBP and Actin - images are too close - TBP band is slightly stretched compared to the Actin band.. The above are proposed candidates for further analysis of image duplication by image experts.. ...
Cell Culture. The human umbilical vascular endothelial cellderived EA.hy926 cell line was used (Edgell et al., 1983). Cells were grown in Dulbeccos modified Eagles medium containing 10% fetal bovine serum and hypoxanthine-aminopterin-thymidine supplement (Sigma-Aldrich, St. Louis, MO). Cells (80-90% confluent) were incubated in serum-free medium for 24 h followed by the addition of 20-HETE (0.1-10 nM) for the indicated times. Cells were harvested and processed for the various measurements. In some experiments, cells (60-70% confluent) were infected with the adenoviral constructs pAd5CMV-NpA-eGFP (Adv-GFP) or pAd5CMV-NpA-CYP4A2 (Adv-CYP4A2) as described previously (Wang et al., 2006). CYP4A2 protein expression was determined by Western blot analysis, and 20-HETE levels measured by gas chromatography/mass spectrometry as described previously (Wang et al., 2006).. Nuclear Extract Preparation. Nuclear proteins were extracted using the CelLytic NuCLEAR extraction kit (Sigma-Aldrich) according to ...
15 Lipoxygenase 1小鼠单克隆抗体[3G8](ab119774)可与大鼠, 狗, 人, 猴样本反应并经WB, IHC, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
(EMAILWIRE.COM, November 13, 2017 ) Europe Arachidonic Acid Market is worth USD xx million in 2016 and estimated to grow at a CAGR of xx.xx%, to reach USD xx million by 2021. The Europe Arachidonic Acid market is developing at an exceptionally quick pace. Browse Market data tables and in-depth...
Arachidonic Acids Role in Stress and Shock April 3, 2012 Posted in General.. Comments Off on Arachidonic Acids Role in Stress and Shock ...
References for Abcams Recombinant Human 15 Lipoxygenase 1 protein (ab114421). Please let us know if you have used this product in your publication
References for Abcams Recombinant Human 5 Lipoxygenase protein (ab114310). Please let us know if you have used this product in your publication
Discovery of selective imidazole-based inhibitors of mammalian 15-lipoxygenase: Highly potent against human enzyme within a cellular environment ...
just read a article about arachidonic acid,,,had sum great info on it,,,,was wondering if anyone here uses it while on / off cycle and does it really work or is
8(S)-Hete/AFI98462034 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Alox15 - Alox15 (untagged) - Mouse arachidonate 15-lipoxygenase (Alox15), (10ug) available for purchase from OriGene - Your Gene Company.
Royo, J., Vancanneyt, G., Perez, A.G., Sanz, C., Stormann, K., Rosahl, S. and Sanchez-Serrano, J.J. (1996). „Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns". J. Biol. Chem. 271: 21012-21019. PMID 8702864 ...
Az autoimmun thyreoiditisre (AT-re) jellemz sz vettani s cytol giai k pet mutat a HT-os betegek pajzsmirigye. Lymphocyt s s plazmasejtes infiltr ci jellemzi, helyenk nt germin lis centrumok, esetleg n. Askenazy-sejtek is l that k. Immunhisztok miai m dszerekkel a thyreoglobulin (Tg), ill. pajzsmirigy-peroxid z (TPO) elleni antitestek is kimutathat ak. Az AT egyes llatokban spont n el fordul, m sokban pedig kiv lthat . A BG-k rnak, ill. a HT-nak megfelel betegs get azonban llatokban nem figyeltek meg. Az emberi megbeteged shez legink bb a csirk kben tanulm nyozhat autoimmun pajzsmirigybetegs g hasonl t. Siker lt ugyanis a Cornell csirket rzsb l (CS) egy olyan t rzset kiteny szteni, amelyben 8-10 hetes korban a Hashimoto-thyreoiditishez hasonl megbeteged s jelentkezik s a pajzsmirigy elleni antitestek titere is emelkedett, az llatok pedig hypothyreosisosakk v lnak (Obese Strain=OS csirke). Ezekben az llatokban a neonatalis bursectomia megakad lyozta, a thymectomia pedig el seg tette a ...
AuxInfo=1/1/N:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22/E:(21,22)/rA:22nCCCCCCCCCCCCCCCCCCCCOO/rB:s1;s2;s3;s4;s5;d6;s7;s8;d9;s10;s11;d12;s13;s14;d15;s16;s17;s18;s19;s20;d20;/rC:-7.3658,-.6188,0;-6.6513,-.2062,0;-5.9368,-.6188,0;-5.2224,-.2062,0;-4.5079,-.6188,0;-3.7934,-.2062,0;-2.9684,-.2062,0;-2.2539,-.6188,0;-1.5395,-.2062,0;-.7145,-.2062,0;0,-.6188,0;.7145,-.2062,0;1.5395,-.2062,0;2.2539,-.6188,0;2.9684,-.2062,0;3.7934,-.2062,0;4.5079,-.6188,0;5.2224,-.2062,0;5.9368,-.6188,0;6.6513,-.2062,0;7.3658,-.6188,0;6.6513,.6188,0 ...
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The problem however, is that "just be yourself" is inherently bad advice. Being authentic is one thing - thats something we all should be doing. But "just be yourself" is about not changing, period. And sometimes, quite frankly, being yourself is the problem. It doesnt do you any good to "just be yourself" if you suck. Being told to be yourself means refusing to change, even when your current self is whats holding you back. Ive lost track of how many people Ive known whose "bad luck" with women boiled down to something about themselves - something that was well within their ability to fix.When Id point out their issue: a shitty attitude towards women, an unrealistic expectation of relationships or just plain being a selfish asshole - theyd come back with "well, women should love me for who I am. Im not going to change just to please people." Then with their very next breath1 theyre back to wondering why women dont like them ...

12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor | definition of 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor...12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor | definition of 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor...

... hydroxy-5,8,10,14-eicosatetraenoic acid receptor in the Medical Dictionary? 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid ... hydroxy-5,8,10,14-eicosatetraenoic acid receptor? Meaning of 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor medical ... What does 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor mean? ... hydroxy-5,8,10,14-eicosatetraenoic acid receptor) GPR31. A gene on chromosome 6q27 that encodes a G protein-coupled protein ...
more infohttps://medical-dictionary.thefreedictionary.com/12-

15-hydroxy-5,8,11,13-eicosatetraenoic acid
     Summary Report | CureHunter15-hydroxy-5,8,11,13-eicosatetraenoic acid Summary Report | CureHunter

13-eicosatetraenoic acid: potent & selective inhibitor of platelet lipoxygenase; RN given refers to cpd without isomeric ... Unsaturated Fatty Acids: 3881*Eicosanoids: 1470*Arachidonic Acids: 92*Hydroxyeicosatetraenoic Acids: 193*15-hydroxy-5,8,11,13- ... 13-eicosatetraenoic acid. Subscribe to New Research on 15-hydroxy-5,8,11,13-eicosatetraenoic acid ... Arachidonic Acids: 92*Hydroxyeicosatetraenoic Acids: 193*15-hydroxy-5,8,11,13-eicosatetraenoic acid: 107 ...
more infohttp://www.curehunter.com/public/keywordSummaryC025984-15-hydroxy-5-8-11-13-eicosatetraenoic-acid.do

In Vivo Inhibition of CC and CX3C Chemokine-induced Leukocyte Infiltration and Attenuation of Glomerulonephritis in Wistar...In Vivo Inhibition of CC and CX3C Chemokine-induced Leukocyte Infiltration and Attenuation of Glomerulonephritis in Wistar...

e-f ) Periodic acid-Schiff staining of kidney section of PBS- (e) or vMIP-II-treated rats (f ). Sections were sampled on day 6 ... 1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156- ... tissues were stained with periodic acid-Schiff reagent. The number of crescentic glomeruli per 100 glomeruli of each rat was ... Figure 5 (a) Proteinuria (milligrams of urine protein per 24 h) and (b) serum creatinine levels (arbitrary units) in WKY rats ...
more infohttp://jem.rupress.org/content/188/1/193?ijkey=c38ce77db747fb01064ee8599370fce88cf60388&keytype2=tf_ipsecsha

12-Hydroxyeicosatetraenoic acid - Wikipedia12-Hydroxyeicosatetraenoic acid - Wikipedia

8R-hydroxy-11R,12R-epoxy-5Z,9E,14Z-eicosatetraenoic acid (i.e. 8R-hydroxy,11R,12R-epoxy-hepoxilin A3 or 8R-OH,11R,12R-epoxy- ... and trihydroxy-eicosatetraenoic acids that possess R or S hydroxy and epoxy residues at various sites while 8R-hydroxy,11R,12R- ... 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (12(S)-HETE or 12S-HETE), made by platelets, but also the later detected "R" ... hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (also termed 12(R)-HETE or 12R-HETE) made by other tissues through their 12R- ...
more infohttps://en.wikipedia.org/wiki/12-Hydroxyeicosatetraenoic_acid

Transmembrane Proteins for Research Use  Page 94  - CusabioTransmembrane Proteins for Research Use Page 94 - Cusabio

For example, non-natural amino acids can be added to the protein expression to realize the complex modification process that is ... hydroxy-5,8,10,14-eicosatetraenoic acid receptor(GPR31). CSB-CF009802HU. in vitro E.coli expression system. ... Recombinant Serpentine receptor class XA 10(srxa-10). CSB-CF330368CXY. in vitro E.coli expression system. ...
more infohttps://www.cusabio.com/catalog-54-94.html

A 12(S)-hydroxyeicosatetraenoic acid receptor interacts with steroid receptor coactivator-1 | PNASA 12(S)-hydroxyeicosatetraenoic acid receptor interacts with steroid receptor coactivator-1 | PNAS

... eicosatetraenoic acid [12(S)-HETE] is formed from arachidonic acid by the enzyme 12-lipoxygenase (1, 2). Platelets, ... hydroxy-5,8,10,14-eicosatetraenoic acid [12(S)-HETE]. These binding sites have a cytosolic/nuclear localization and contain the ... 4C). Three C-terminal deletion mutants [SRC-1/AatII (amino acids 1-1,393), SRC-1/NheI (amino acids 1-947), and SRC-1/BamHI ( ... hydroxyeicosatetraenoic acid;. LLC,. Lewis lung carcinoma;. LXD,. domain having one LXXLL motif (X meaning any amino acid);. ...
more infohttp://www.pnas.org/content/97/11/5779

Preliminary Findings on the Patterns of Epidermal Damage Induced by Irritants in Man | Springer for Research & DevelopmentPreliminary Findings on the Patterns of Epidermal Damage Induced by Irritants in Man | Springer for Research & Development

... nonanoic acid, dithranol, and propylene glycol has been investigated. Patterns of epidermal damage were identified for each ... 1985) Cutaneous responses to 12 hydroxy - 5, 8, 10, 14 eicosatetraenoic acid (12 HETE) J Invest Dermatol 84: 537-541.PubMed ...
more infohttps://rd.springer.com/chapter/10.1007/978-3-642-74299-6_7

Inhibition of the Biosynthesis of Prostaglandin E2 By Low-Dose Aspirin: Implications for Adenocarcinoma Metastasis | Cancer...Inhibition of the Biosynthesis of Prostaglandin E2 By Low-Dose Aspirin: Implications for Adenocarcinoma Metastasis | Cancer...

... the same concentration of exogenous arachidonic acid was used for all experiments, (3) deuterated arachidonic acid was used to ... Volunteers were given 2 weeks of aspirin 81 mg daily, and the urinary metabolites of PGE2 (11α-hydroxy-9,15-dioxo-2,3,4,5- ... PRP was acidified to pH 6.4 with 0.15 mol/L citric acid, and centrifuged at 1,000 x g for 10 minutes at room temperature. The ... Prevention of NNK-induced lung tumorigenesis in A/J mice by acetylsalicylic acid and NS-398. Cancer Res 1998;58:5354-60. ...
more infohttp://cancerpreventionresearch.aacrjournals.org/content/9/11/855.long

DiVA - Search resultDiVA - Search result

4-hydroxy-3-(1-methylethyl)-phenoxy]-phenyl]-amino]-3-oxopropanoic acid)] in humans. In moderately overweight and ... The second interaction domain was present in the N-terminal part of SRC-1 (amino acids 1-221). This region has two LXXLL motifs ... Bile acid synthesis was stimulated without evidence of increased cholesterol production, indicating that KB2115 induced net ... A subsequent database search revealed that the amino acid sequence of a lysine-rich C-terminal segment of HP0508 is identical ...
more infohttp://liu.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22journalId%22%3A%2215041%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=

尋找研究成果
             - 臺北醫學大學尋找研究成果 - 臺北醫學大學

Effects of purified eicosapentaenoic acid on arachidonic acid metabolism in cultured murine aortic smooth muscle cells, vessel ... Javaherian, K. & Liu, L-F., 一月 25 1983, 於 : Nucleic Acids Research. 11, 2, p. 461-472 12 p.. 研究成果: 雜誌貢獻 › 文章 ... Induction of fatty acid cyclooxygenase in rat aortic smooth muscle cells by estradiol. Chang, W. C., Nakao, J., Murota, S. I ... of arachidonic acid. Nakao, J., Ito, H., Chang, W. C., Koshihara, Y. & Murota, S. I., 五月 16 1983, 於 : Biochemical and ...
more infohttps://tmu.pure.elsevier.com/zh/publications/?descending=false&showAdvanced=false&allConcepts=true&inferConcepts=true&originalSearch=&improvedLayoutOrganisationUuid=&format=&page=7

Cigarette smoking stimulates lipoxygenase but not cyclooxygenase pathway in platelets<...Cigarette smoking stimulates lipoxygenase but not cyclooxygenase pathway in platelets<...

Male rats were exposed to freshly generated cigarette smoke once daily for 4 to 8 weeks. Inhalation of smoke was verified by ... N2 - Male rats were exposed to freshly generated cigarette smoke once daily for 4 to 8 weeks. Inhalation of smoke was verified ... AB - Male rats were exposed to freshly generated cigarette smoke once daily for 4 to 8 weeks. Inhalation of smoke was verified ... abstract = "Male rats were exposed to freshly generated cigarette smoke once daily for 4 to 8 weeks. Inhalation of smoke was ...
more infohttps://tmu.pure.elsevier.com/en/publications/cigarette-smoking-stimulates-lipoxygenase-but-not-cyclooxygenase-

Src-dependent STAT-3-mediated expression of monocyte chemoattractant protein-1 is required for 15(S)-hydroxyeicosatetraenoic...Src-dependent STAT-3-mediated expression of monocyte chemoattractant protein-1 is required for 15(S)-hydroxyeicosatetraenoic...

... we have studied the effect of the major 15-LOX1 metabolite of arachidonic acid, 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE ... we have studied the effect of the major 15-LOX1 metabolite of arachidonic acid, 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE ... we have studied the effect of the major 15-LOX1 metabolite of arachidonic acid, 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE ... we have studied the effect of the major 15-LOX1 metabolite of arachidonic acid, 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE ...
more infohttps://uthsc.pure.elsevier.com/en/publications/src-dependent-stat-3-mediated-expression-of-monocyte-chemoattract

Urticaria/Angioedema | SpringerLinkUrticaria/Angioedema | SpringerLink

Farr, R. S., 1970, The need to re-evaluate acetyl salicylic acid (aspirin), J. Allergy 45: 321-328.PubMedCrossRefGoogle Scholar ... Champion, R. H., and Lachmann, P. J., 1969, Hereditary angio-oedema treated with e-aminocaproic acid, Br. J. Dermatol 81: 763- ... Sheffer, A. L., Austen, K. F., and Rosen, F. S., 1972, Tranexamic acid therapy in hereditary angioneurotic edema, N. Engl. J. ... Pence, H. L., Evans, R., Guernsey, L. H., and Gerhard, R. C., 1974, Prophylactic use of epsilon aminocaproic acid for oral ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4615-7228-2_17

Plus itPlus it

... eicosatetraenoic acid; LTD4 and LTC4 did not displace the ligand in concentrations up to 50 microM. The binding was inhibited ... The relative potencies of LTB4 analogs to displace the tritiated ligand were LTB4 greater than 20-hydroxy-LTB4 much greater ... than 20-carboxy-LTB4 greater than 12-(S)hydroxy-5,8,10,14(Z,Z,E,Z)- ...
more infohttp://jpet.aspetjournals.org/content/259/3/1035

As such, import of proteins containing a classical NLS is i - aunt high topAs such, import of proteins containing a classical NLS is i - aunt high top

In vivo transformation of arachidonic acid into 12-hydroxy-5,8,10,14-eicosatetraenoic acid by human nasal mucosa. Complex ... to begin with small doses and slowly increase them gradually to reach the level of adult dosage within 4-5 years. Participants ... http://tollcrack.site/to-evaluate-associations-between-docosahexaenoic-acid-dha-and/. http://goalrider.top/furthermore-we- ... http://enterothherss35.xyz/our-findings-suggest-that-il-8-plays-a-rol/. http://gaintool.icu/the-absence-of-consistent- ...
more infohttp://aunthigh.top/as-such-import-of-proteins-containing-a-classical-nls-is-i/

NAVER 학술정보 >...NAVER 학술정보 >...

15-Hydroperoxyeicosapentaenoic acid inhibits arachidonic acid metabolism in rabbit platelets more potently than ... Animals, Brain Chemistry, Dietary Fats, administration & dosage, Fatty Acids, Omega-3, analysis, Fatty Acids, Unsaturated, ... Animals, Ceramides, chemistry, physiology, Cholesterol, Epidermis, Lipids, Palmitic Acid, Palmitic Acids, Solubility, Swine, ... Arachidonic Acid, blood, metabolism, Blood Platelets, drug effects, Eicosapentaenoic Acid, analogs & derivatives,... ...
more infohttps://academic.naver.com/search.naver?field=3&query=Biochimica+et+Biophysica+Acta+1300%EA%B6%8C+3%ED%98%B8

List of MeSH codes (D10) - WikipediaList of MeSH codes (D10) - Wikipedia

... oleic acids MeSH D10.251.355.325.600.525 --- oleic acid MeSH D10.251.355.325.600.780 --- ricinoleic acids MeSH D10.251.355.325. ... 15-hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic acid MeSH D10.251.355.255.100.637.100 --- prostaglandins a MeSH ... fatty acids, omega-6 MeSH D10.251.355.343.249 --- gamma-linolenic acid MeSH D10.251.355.343.500 --- linoleic acids MeSH D10.251 ... gamma-linolenic acid MeSH D10.251.355.840 --- sorbic acid MeSH D10.251.355.920 --- trans fatty acids MeSH D10.251.400.092 --- ...
more infohttps://en.wikipedia.org/wiki/List_of_MeSH_codes_(D10)

Human Metabolome Database: Showing metabocard for Prostaglandin F2a (HMDB0001139)Human Metabolome Database: Showing metabocard for Prostaglandin F2a (HMDB0001139)

Increased concentrations of nonesterified arachidonic acid, 12L-hydroxy-5,8,10,14-eicosatetraenoic acid, prostaglandin E2, and ... and are based upon the fatty acid arachidonic acid. ... 9,11,15-Trihydroxyprosta-5Z,13E-dien-1-Oic acid. HMDB. 9a,11a, ... 9alpha,11alpha,15)-Trihydroxyprosta-(5Z,13E)-dien-1-Oic acid. HMDB. 7-[3,5-Dihydroxy-2-(3-hydroxy-1-octenyl)cyclopentyl]-5- ... These are unsaturated carboxylic acids consisting of a 20 carbon skeleton that also contains a five member ring, ...
more infohttp://www.hmdb.ca/metabolites/HMDB0001139

Table of Contents - December 01, 2008, 74 (6) | Molecular PharmacologyTable of Contents - December 01, 2008, 74 (6) | Molecular Pharmacology

... eicosatetraenoic Acid [12(R)-HETE], an Arachidonic Acid Derivative, Is an Activator of the Aryl Hydrocarbon Receptor ... Potent Activation of Large-Conductance Ca2+-Activated K+ Channels by the Diphenylurea 1,3-Bis-[2-hydroxy-5-(trifluoromethyl) ... The Orphan Transporter Rxt1/NTT4 (SLC6A17) Functions as a Synaptic Vesicle Amino Acid Transporter Selective for Proline, ... Dissecting the Role of Multiple Reductases in Bioactivation and Cytotoxicity of the Antitumor Agent 2,5-Diaziridinyl-3-( ...
more infohttp://molpharm.aspetjournals.org/content/74/6

Platelet Signaling Pathways and New Inhibitors | Arteriosclerosis, Thrombosis, and Vascular BiologyPlatelet Signaling Pathways and New Inhibitors | Arteriosclerosis, Thrombosis, and Vascular Biology

... hydroxy-8Z,10E,14Z-eicosatrienoic acid (Figure). Administration of dihomo-γ-linolenic acid or 12(S)-hydroxy-8Z,10E,14Z- ... 12S-hydroxy-8Z,10E,14Z-eicosatrienoic acid; 12-LOX, 12-lipoxygenase; AC, adenylyl cyclase; FCγR, Fcγ receptor; FXR, farnesoid X ... LOXs (lipoxygenases) are a family of enzymes that catalyze the oxygenation of polyunsaturated fatty acids that leads to the ... Membrane-potential-dependent inhibition of platelet adhesion to endothelial cells by epoxyeicosatrienoic acids. Arterioscler ...
more infohttp://atvb.ahajournals.org/content/38/4/e28

気管支喘息における好酸球動態の調節に関する研究 第2編 気管支喘息における好酸球動態の調節に関する研究 第2編

10) 河田一郎:気管支喘息の発症機序における好酸球動態に関する研究-第2編カンジダ抗原による末梢血単核球由来好酸球遊走因子について.岡山医誌 (1990) 102, 745-755.. 11) 河田一郎:気管支喘息の発症機序における好酸球の動態に関する ... 8) 木村郁郎:気管支肺病変におけるアレルギーとリンパ球.アレルギア (1990) 19, 12-16.. 9) 宮川秀文,難波一弘,白石高昌,名部 誠,槇本 晃,佐藤 恭,
more infohttp://eprints.lib.okayama-u.ac.jp/en/14178

Role of arachidonic acid metabolites on the control of non-differentiated intestinal epithelial cell growth.  - PubMed - NCBIRole of arachidonic acid metabolites on the control of non-differentiated intestinal epithelial cell growth. - PubMed - NCBI

2013 Aug;45(8):1620-8. doi: 10.1016/j.biocel.2013.05.009. Epub 2013 May 16. Research Support, Non-U.S. Govt ... epoxyeicosatrienoic acid; hydroxyeicosatetraenoic acid; leukotriene; lipoxygenase; nordihydroguaiaretic acid; phospholipase A(2 ... Role of arachidonic acid metabolites on the control of non-differentiated intestinal epithelial cell growth.. Cabral M1, Martín ... Increasingly evidence indicates that enzymes, receptors and metabolites of the arachidonic acid (AA) cascade play a role in ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/23685077

Direct opening of S-type K+ channels of Aplysia sensory neurons by 12-lipoxygenase metabolites - ServalDirect opening of S-type K+ channels of Aplysia sensory neurons by 12-lipoxygenase metabolites - Serval

... metabolism Hydroxyeicosatetraenoic Acids/physiology Molecular Sequence Data Neurons, Afferent/*metabolism Potassium Channels/* ... Direct opening of S-type K+ channels of Aplysia sensory neurons by 12-lipoxygenase metabolites. Details. Serval ID ... 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid Amino Acid Sequence Animals Aplysia/*metabolism Arachidonate 12-Lipoxygenase/* ... Direct opening of S-type K+ channels of Aplysia sensory neurons by 12-lipoxygenase metabolites ...
more infohttps://serval.unil.ch/notice/serval:BIB_CD2EE364FD34

Arachidonate 5-lipoxygenase - WikipediaArachidonate 5-lipoxygenase - Wikipedia

... hydroxy-6E,8Z,11Z-eicosatrienoic acid (5-HETrE), 5-oxo-6,8,11-eicosatrienoic acid (5-oxo-ETrE), LTA3, and LTC3; since LTA3 ... Eicosapentaenoic acid[edit]. ALOX5 metabolizes the omega-3 fatty acid, Eicosapentaenoic acid (EPA, i.e. 4Z,8Z,11Z,14Z,17Z- ... Arachidonic acid[edit]. ALOX5 metabolizes the omega-6 fatty acid, Arachidonic acid (AA, i.e. 5Z,8Z,11Z,15Z-eicosatrienoic acid ... Docosahexaenoic acid[edit]. ALOX5 acts in series with ALOX15 to metabolize the omega 3 fatty acid, docosahexaenoic acid (DHA, i ...
more infohttps://en.wikipedia.org/wiki/Arachidonate_5-lipoxygenase

ChemIDplus - 142828-12-4 - RIUDRKHGFDAKPO-WWWYWCMOSA-N - 5-Oxo-15-hydroxy-6,8,11,13-eicosatetraenoic acid - Similar structures...ChemIDplus - 142828-12-4 - RIUDRKHGFDAKPO-WWWYWCMOSA-N - 5-Oxo-15-hydroxy-6,8,11,13-eicosatetraenoic acid - Similar structures...

13-eicosatetraenoic acid - Similar structures search, synonyms, formulas, resource links, and other chemical information. ... 13-Eicosatetraenoic acid, 15-hydroxy-5-oxo-, (S-(E,E,Z,Z))- ... 12-13,16-17H2,1H3,(H,23,24)/b7-5-,8-6-,14-10+,15-11+/t18-/m0/s1 ... 13-eicosatetraenoic acid. RN: 142828-12-4. InChIKey: RIUDRKHGFDAKPO-WWWYWCMOSA-N. Note. *. A potent eosinophil chemotactic ...
more infohttps://chem.nlm.nih.gov/chemidplus/rn/142828-12-4
  • Lewis lung carcinoma (LLC) and B 16 melanoma], to metastasize, apparently as a consequence of increased expression of a cell adhesion glycoprotein ( 8 ). (pnas.org)
  • One such viral protein, vMIP-II, encoded by human herpesvirus 8, has been identified with in vitro antagonistic activities against CC and CXC chemokine receptors. (rupress.org)
  • The ALOX5 gene, which occupies 71.9 kilo base pairs (kb) on chromosome 10 (all other human lipoxygenases are clustered together on chromosome 17), is composed of 14 exons divided by 13 introns encoding the mature 78 kilodalton (kD) ALOX5 protein consisting of 673 amino acids. (wikipedia.org)
  • Nuclear receptors are ligand-dependent transcription factors that bind as dimers to specific DNA sequences in promoter regions of target genes ( 13 , 14 ). (pnas.org)
  • The gene promoter region of ALOX5 contains 8 GC boxes but lacks TATA boxes or CAT boxes and thus resembles the gene promoters of typical housekeeping genes . (wikipedia.org)
  • Both observational studies ( 1-5 ) and controlled clinical trials ( 6 ) support the concept that aspirin prevents mortality and metastasis from adenocarcinomas. (aacrjournals.org)
  • Similar clinical syndromes may occur after direct nonimmunological mast cell degranulation or in association with abnormalities of the complement pathways or the arachidonic acid-prostaglandin systems. (springer.com)
  • Quality and accuracy of the mobile ECG solution CardioSecur has been validated in a clinical study against standard of care 10 electrode, 12-lead ECG systems and shows 99. (freethesaurus.com)
  • To evaluate the reactivity of eosinophils in asthmatics under various conditions, the migratory function of eosinophils to PAF and IL-5 was investigated by the modified Boyden chamber method. (okayama-u.ac.jp)
  • These findings suggest that the reactivity of eosinophils is heterogenous and relates to the degree of eosinophilia, and that IL-5 as well as PAF plays an important role in the pathogenesis of bronchial asthma. (okayama-u.ac.jp)
  • 5) Wang JW, Rambaldi A, Biondi A, Chen ZG, Sanderson CJ and Montovani A : Recombinant human interleukin 5 is a selective eosinophil chemoattractant. (okayama-u.ac.jp)
  • to begin with small doses and slowly increase them gradually to reach the level of adult dosage within 4-5 years. (aunthigh.top)
  • In contrast, SPIRIT RECOVERY MEDICINE BAG is arranged around health and wholeness, and offers 12 step programs and insights on how real transformation is achieved. (thefreedictionary.com)