An enzyme that catalyzes the oxidation of 1-pyrroline-5-carboxylate to L-GLUTAMATE in the presence of NAD. Defects in the enzyme are the cause of hyperprolinemia II.
A group of enzymes that catalyze the reduction of 1-pyrroline carboxylate to proline in the presence of NAD(P)H. Includes both the 2-oxidoreductase (EC 1.5.1.1) and the 5-oxidoreductase (EC 1.5.1.2). The former also reduces 1-piperidine-2-carboxylate to pipecolate and the latter also reduces 1-pyrroline-3-hydroxy-5-carboxylate to hydroxyproline.
The first enzyme of the proline degradative pathway. It catalyzes the oxidation of proline to pyrroline-5-carboxylic acid in the presence of oxygen and water. The action is not reversible. The specific activity of proline oxidase increases with age. EC 1.5.3.-.
Enzymes catalyzing the dehydrogenation of secondary amines, introducing a C=N double bond as the primary reaction. In some cases this is later hydrolyzed.
A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.
A metabolite in the principal biochemical pathway of lysine. It antagonizes neuroexcitatory activity modulated by the glutamate receptor, N-METHYL-D-ASPARTATE; (NMDA).
A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the transfer of amino group of L-LYSINE onto 2-oxoglutarate to generate 2-aminoadipate 6-semialdehyde and L-GLUTAMATE.
Heterocyclic compounds in which an oxygen is attached to a cyclic nitrogen.
A technique for detecting short-lived reactive FREE RADICALS in biological systems by providing a nitrone or nitrose compound for an addition reaction to occur which produces an ELECTRON SPIN RESONANCE SPECTROSCOPY-detectable aminoxyl radical. In spin trapping, the compound trapping the radical is called the spin trap and the addition product of the radical is identified as the spin adduct. (Free Rad Res Comm 1990;9(3-6):163)
Molecules which contain an atom or a group of atoms exhibiting an unpaired electron spin that can be detected by electron spin resonance spectroscopy and can be bonded to another molecule. (McGraw-Hill Dictionary of Chemical and Technical Terms, 4th ed)
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
Proteins found in any species of bacterium.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Inorganic oxides that contain nitrogen.
A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen.
The univalent radical OH. Hydroxyl radical is a potent oxidizing agent.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies produced by a single clone of cells.
A genus of gram-negative, anaerobic, metal-reducing bacteria in the family Geobacteraceae. They have the ability to oxidize a variety of organic compounds, including AROMATIC HYDROCARBONS.
Electric power supply devices which convert biological energy, such as chemical energy of metabolism or mechanical energy of periodic movements, into electrical energy.
A group of PROTEOBACTERIA represented by morphologically diverse, anaerobic sulfidogens. Some members of this group are considered bacterial predators, having bacteriolytic properties.
Inorganic or organic compounds containing trivalent iron.
The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.
The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.
A cyclized derivative of L-GLUTAMIC ACID. Elevated blood levels may be associated with problems of GLUTAMINE or GLUTATHIONE metabolism.
An NADP+ dependent enzyme that catalyzes the oxidation of L-glutamate 5-semialdehyde to L-glutamyl 5-phosphate. It plays a role in the urea cycle and metabolism of amino groups.
Proteins encoded by the mitochondrial genome or proteins encoded by the nuclear genome that are imported to and resident in the MITOCHONDRIA.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)

Molecular enzymology of mammalian Delta1-pyrroline-5-carboxylate synthase. Alternative splice donor utilization generates isoforms with different sensitivity to ornithine inhibition. (1/62)

Delta1-Pyrroline-5-carboxylate synthase (P5CS; EC not assigned), a mitochondrial inner membrane, ATP- and NADPH-dependent, bifunctional enzyme, catalyzes the reduction of glutamate to Delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline and ornithine. We utilized published plant P5CS sequence to search the expressed sequence tag data base and cloned two full-length human P5CS cDNAs differing in length by 6 base pairs (bp) in the open reading frame. The short cDNA has a 2379-bp open reading frame encoding a protein of 793 residues; the long cDNA, generated by "exon sliding," a form of alternative splicing, contains an additional 6-bp insert following bp +711 of the short form resulting in inclusion of two additional amino acids in the region predicted to be the gamma-glutamyl kinase active site of P5CS. The long form predominates in all tissues examined except gut. We also isolated the corresponding long and short murine P5CS transcripts. To confirm the identity of the putative P5CS cDNAs, we expressed both human forms in gamma-glutamyl kinase- and gamma-glutamyl phosphate reductase-deficient strains of Saccharomyces cerevisiae and showed that they conferred the proline prototrophy. Additionally, we found expression of the murine putative P5CS cDNAs conferred proline prototrophy to P5CS-deficient Chinese hamster ovary cells (CHO-K1). We utilized stable CHO-K1 cell transformants to compare the biochemical characteristics of the long and short murine P5CS isoforms. We found that both confer P5CS activity and that the short isoform is inhibited by L-ornithine with a Ki of approximately 0.25 mM. Surprisingly, the long isoform is insensitive to ornithine inhibition. Thus, the two amino acid insert in the long isoform abolishes feedback inhibition of P5CS activity by L-ornithine.  (+info)

The sfr6 mutation in Arabidopsis suppresses low-temperature induction of genes dependent on the CRT/DRE sequence motif. (2/62)

The sfr mutations, which result in sensitivity to freezing after cold acclimation, define genes that are required for freezing tolerance. We tested plants homozygous for mutations sfr2 to sfr7 for cold-induced gene expression and found that sfr 6 plants were deficient in cold-inducible expression of the genes KIN1, COR15a, and LTI78, which all contain the C repeat/dehydration-responsive element (CRT/DRE) motif in their promoters. Similarly, sfr 6 plants failed to induce KIN1 normally in response to either osmotic stress or the application of abscisic acid. In contrast, cold-inducible expression of genes CBF1, CBF2, CBF3, and ATP5CS1, which lack the CRT/DRE motif, was not affected. The freezing-sensitive phenotype that defines sfr 6 also was found to be tightly linked to the gene expression phenotype. To determine whether the failure of cold induction of CRT/DRE-containing genes in sfr 6 was due to altered low-temperature calcium signaling, cold-induced cytosolic-free calcium ([Ca2+]cyt) elevations were investigated in the sfr 6 mutant, but these were found to be indistinguishable from those of the wild type. We discuss the possibilities that CRT/DRE binding proteins (such as CBF1) require activation to play a role in transcription and that the SFR6 protein is a vital component of their activation.  (+info)

Proline accumulation in developing grapevine fruit occurs independently of changes in the levels of delta1-pyrroline-5-carboxylate synthetase mRNA or protein. (3/62)

Mature fruit of grapevine (Vitis vinifera) contains unusually high levels of free proline (Pro; up to 24 micromol or 2.8 mg/g fresh weight). Pro accumulation does not occur uniformly throughout berry development but only during the last 4 to 6 weeks of ripening when both berry growth and net protein accumulation have ceased. In contrast, the steady-state levels of both the mRNA encoding V. vinifera Delta1-pyrroline-5-carboxylate synthetase (VVP5CS), a key regulatory enzyme in Pro biosynthesis, and its protein product remain relatively uniform throughout fruit development. In addition, the steady-state protein levels of Pro dehydrogenase, the first enzyme in Pro degradation, increased throughout early fruit development but thereafter remained relatively constant. The developmental accumulation of free Pro late in grape berry ripening is thus clearly distinct from the osmotic stress-induced accumulation of Pro in plants. It is not associated with either sustained increases in steady-state levels of P5CS mRNA or protein or a decrease in steady-state levels of Pro dehydrogenase protein, suggesting that other physiological factors are important for its regulation.  (+info)

Metabolite repression and inducer exclusion in the proline utilization gene cluster of Aspergillus nidulans. (4/62)

The clustered prnB, prnC, and prnD genes are repressed by the simultaneous presence of glucose and ammonium. A derepressed mutation inactivating a CreA-binding site acts in cis only on the permease gene (prnB) while derepression of prnD and prnC is largely the result of reversal of inducer exclusion.  (+info)

Removal of feedback inhibition of delta(1)-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. (5/62)

The Delta(1)-pyrroline-5-carboxylate synthetase (P5CS; EC not assigned) is the rate-limiting enzyme in proline (Pro) biosynthesis in plants and is subject to feedback inhibition by Pro. It has been suggested that the feedback regulation of P5CS is lost in plants under stress conditions. We compared Pro levels in transgenic tobacco (Nicotiana tabacum) plants expressing a wild-type form of Vigna aconitifolia P5CS and a mutated form of the enzyme (P5CSF129A) whose feedback inhibition by Pro was removed by site-directed mutagenesis. Transgenic plants expressing P5CSF129A accumulated about 2-fold more Pro than the plants expressing V. aconitifolia wild-type P5CS. This difference was further increased in plants treated with 200 mM NaCl. These results demonstrated that the feedback regulation of P5CS plays a role in controlling the level of Pro in plants under both normal and stress conditions. The elevated Pro also reduced free radical levels in response to osmotic stress, as measured by malondialdehyde production, and significantly improved the ability of the transgenic seedlings to grow in medium containing up to 200 mM NaCl. These findings shed new light on the regulation of Pro biosynthesis in plants and the role of Pro in reducing oxidative stress induced by osmotic stress, in addition to its accepted role as an osmolyte.  (+info)

Genetic manipulation of the metabolism of polyamines in poplar cells. The regulation of putrescine catabolism. (6/62)

We investigated the catabolism of putrescine (Put) in a non-transgenic (NT) and a transgenic cell line of poplar (Populus nigra x maximowiczii) expressing a mouse (Mus musculus) ornithine (Orn) decarboxylase (odc) cDNA. The transgenic cells produce 3- to 4-fold higher amounts of Put than the NT cells. The rate of loss of Put from the cells and the initial half-life of cellular Put were determined by feeding the cells with [U-(14)C]Orn and [1,4-(14)C]Put as precursors and following the loss of [(14)C]Put in the cells at various times after transfer to label-free medium. The amount of Put converted into spermidine as well as the loss of Put per gram fresh weight were significantly higher in the transgenic cells than the NT cells. The initial half-life of exogenously supplied [(14)C]Put was not significantly different in the two cell lines. The activity of diamine oxidase, the major enzyme involved in Put catabolism, was comparable in the two cell lines even though the Put content of the transgenic cells was severalfold higher than the NT cells. It is concluded that in poplar cells: (a) exogenously supplied Orn enters the cells and is rapidly converted into Put, (b) the rate of Put catabolism is proportional to the rate of its biosynthesis, and (c) the increased Put degradation occurs without significant changes in the activity of diamine oxidase.  (+info)

A plant gene up-regulated at rust infection sites. (7/62)

Expression of the fis1 gene from flax (Linum usitatissimum) is induced by a compatible rust (Melampsora lini) infection. Infection of transgenic plants containing a beta-glucuronidase (GUS) reporter gene under the control of the fis1 promoter showed that induction is highly localized to those leaf mesophyll cells within and immediately surrounding rust infection sites. The level of induction reflects the extent of fungal growth. In a strong resistance reaction, such as the hypersensitive fleck mediated by the L6 resistance gene, there is very little fungal growth and a microscopic level of GUS expression. Partially resistant flax leaves show levels of GUS expression that were intermediate to the level observed in the fully susceptible infection. Sequence and deletion analysis using both transient Agrobacterium tumefaciens expression and stable transformation assays have shown that the rust-inducible fis1 promoter is contained within a 580-bp fragment. Homologs of fis1 were identified in expressed sequence tag databases of a range of plant species including dicots, monocots, and a gymnosperm. Homologous genes isolated from maize (Zea mays; mis1), barley (Hordeum vulgare; bis1), wheat (Triticum aestivum; wis1), and Arabidopsis encode proteins that are highly similar (76%-82%) to the FIS1 protein. The Arabidopsis homologue has been reported to encode a delta1-pyrroline-5-carboxylate dehydrogenase that is involved in the catabolism of proline to glutamate. RNA-blot analysis showed that mis1 in maize and the bis1 homolog in barley are both up-regulated by a compatible infection with the corresponding species-specific rust. The rust-induced genes homologous to fis1 are present in many plants. The promoters of these genes have potential roles for the engineering of synthetic rust resistance genes by targeting transgene expression to the sites of rust infection.  (+info)

Molecular mechanisms of proline-mediated tolerance to toxic heavy metals in transgenic microalgae. (8/62)

Pro has been shown to play an important role in ameliorating environmental stress in plants and microorganisms, including heavy metal stress. Here, we describe the effects of the expression of a mothbean delta(1)-pyrroline-5-carboxylate synthetase (P5CS) gene in the green microalga Chlamydomonas reinhardtii. We show that transgenic algae expressing the mothbean P5CS gene have 80% higher free-Pro levels than wild-type cells, grow more rapidly in toxic Cd concentrations (100 microM), and bind fourfold more Cd than wild-type cells. In addition, Cd-K edge extended x-ray absorption fine structure studies indicated that Cd does not bind to free Pro in transgenic algae with increased Pro levels but is coordinated tetrahedrally by sulfur of phytochelatin. In contrast to P5CS-expressing cells, Cd is coordinated tetrahedrally by two oxygen and two sulfur atoms in wild-type cells. Measurements of reduced/oxidized GSH ratios and analyses of levels of malondialdehyde, a product of the free radical damage of lipids, indicate that free Pro levels are correlated with the GSH redox state and malondialdehyde levels in heavy metal-treated algae. These results suggest that the free Pro likely acts as an antioxidant in Cd-stressed cells. The resulting increased GSH levels facilitate increased phytochelatin synthesis and sequestration of Cd, because GSH-heavy metal adducts are the substrates for phytochelatin synthase.  (+info)

Matki Indian Moth Bean Vigna Aconitifolia Seeds Packet of 30+ freshly harvested home grown seeds! This is a very handy little nitrogen fixing legume that you dont see grown in Australia very often. Its native to India and Pakistan it is super drought tolerant. Over there they grown heaps both as a food crop and […]
Vigna aconitifolia is a drought-resistant legume, commonly grown in arid and semi-arid regions of India. It is commonly called mat bean, moth bean, matki, Turkish gram or dew bean. The pods, sprouts and protein rich seeds of this crop are commonly consumed in India. Moth bean can be grown on many soil types, and can also act as a pasture legume. Moth bean is an herbaceous creeping annual which grows to approximately 40 cm high. Yellow flowers on its hairy and densely packed branches develop into yellow-brown pods, 2 to 3 inches in length The seeds of these pods contain approximately 22-24% protein. Due to its drought resistant qualities, its ability to combat soil erosion and its high protein content, moth bean has been identified as possibly a more significant food source in the future. It has been suggested that its suitability as a grain legume in semi-arid Africa should be further investigated. Belonging to the family Fabaceae (sub-family Papilionaceae), the moth bean is an herbaceous ...
Inactivation of Surface Microorganisms of Moth bean (Vigna aconitifolia) by Micronization and Changes in its Physico Chemical and Rheological Properties
Glutamic gamma-semialdehyde is the metabolic precursor for proline biosynthesis. The conversion from L-Glutamate, an ATP- and NADPH-dependent reaction, is catalyzed by the enzyme Delta-1-pyrroline-5-carboxylate synthetase (P5CS) (OMIM 138250 ). L-Glutamic-gamma-semialdehyde can also be converted to or be formed from the amino acids L-ornithine (EC 2.6.1.13) and L-proline (EC 1.5.99.8 and EC 1.5.1.2). It is also one of the few metabolites that can be a precursor to other metabolites of both the urea cycle and the citric acid cycle (BioCyc ...
The 45-days-old seedlings of drought resistant (N-22, CR143-2-2) and susceptible rice (Oryza sativa L.) genotypes (Panidhan, Pusa-169) were subjected to osmotic stress in PEG-6000 solution of -10 and -16 bar and the relative water content (RWC), proline content, and pyrroline-5-carboxylate synthetase (P5CS) activity and its P5CS expression were studied. A gradual decrease in RWC was observed in tolerant genotypes, whereas the decrease was drastic in susceptible ones. Proline content and P5CS activity increased both in susceptible and tolerant genotypes; the increase was higher in tolerant genotypes. Higher proline levels in tolerant genotypes were due to increased P5CS activity. The EcoRI, BamHI and XbaI restricted DNA of N-22 and Panidhan genotypes were hybridized with Arabidopsis P5CS sequence and a single band (approx 2.4 kb) was observed, however, P5CS expression was more in N-22, as compared to Panidhan ...
Read Characterization of the gene for Δ1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L., Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Yellow mosaic virus (YMV) causes a greater loss of electrolytes from infected leaf tissues ofVigna aconitifolia (mothbean). Four highly susceptible entries of mothbean were examined for the pattern of electrolyte loss after virus has invaded the tissues. It was observed that YMV triggered a heavy loss of ions at initial stages of disease development, but the loss receded at advanced stages of infection. Maximal damage to electrolytes occurred at the second stage, showing about 50% infection. The findings are interesting as the present observations on viral disease differ from other plant diseases.
Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of ...
CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): In higher eukaryotes, miRNAs and siRNAs guide translational inhibition, mRNA cleavage, or chromatin regulation. We found that the antisense overlapping gene pair of D 1-pyrroline-5-carboxylate dehydrogenase (P5CDH), a stress-related gene, and SRO5, a gene of unknown function, generates two types of siRNAs. When both transcripts are present, a 24-nt siRNA is formed by a biogenesis pathway dependent on DCL2, RDR6, SGS3, and NRPD1A. Initial cleavage of the P5CDH transcript guided by the 24-nt siRNA establishes a phase for the subsequent generation of 21-nt siRNAs by DCL1 and further cleavage of P5CDH transcripts. The expression of SRO5 is induced by salt, and this induction is required to initiate siRNA formation. Our data suggest that the P5CDH and SRO5 proteins are also functionally related, and that the P5CDH-SRO5 gene pair defines a mode of siRNA function and biogenesis that may be applied to other natural cis-antisense gene
1-Pyrroline-5-carboxylic acid is an enamine or an imino acid that forms on spontaneous dehydration of L-glutamate γ-semialdehyde in aqueous solutions. The stereoisomer (S)-1-Pyrroline-5-carboxylate is an intermediate in glutamate metabolism, in arginine degradation and in proline biosynthesis and degradation and it can be converted to or be formed from the three amino acids L-glutamate, L-ornithine and L-proline. In particular, it is synthesized with the oxidation of proline by pyrroline-5-carboxylate reductase 1 (EC 1.5.1.2, PYCR1) or by proline dehydrogenase (EC 1.5.99.8, PRODH) and it is hydrolyzed to L-glutamate by delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12, ALDH4A1). It is also one of the few metabolites that can be a precursor to other metabolites of both the urea cycle and the tricarboxylic acid (TCA) cycle ...
Other articles where Bambara groundnut is discussed: Fabales: Ecological and economic importance: …family is Vigna subterranea (Bambara groundnut), a leguminous plant that develops underground fruits in the arid lands of Africa. Important too are the seeds of Bauhinia esculenta; they are gathered for the high-protein tubers and seeds. Vigna aconitifolia (moth bean) and V. umbellata (rice bean) are much used in…
This nighttime treatment formulation combines 0.5% pure retinol paired with antioxidants and a host of beneficial ingredients to hydrate and soothe, while minimizing the appearance of fine lines and wrinkles. Retinol (Vitamin A) - is converted to retinoic acid in the skin. Vitamin A helps to promote a clear complexion and an even skin tone.Vigna Aconitifolia Seed Extract - is a botanical that promotes a clear complexion and an even skin tone.Sodium Hyaluronate - has the ability to hold 1,000 times its weight in water plays an important role in skin hydration.Glycerophosphoinositol Lysine - is a skin calming agent.Superoxide Dismutase (SOD) - is an enzyme that supports antioxidant defense.
Other articles where Moth bean is discussed: Fabales: Ecological and economic importance: Vigna aconitifolia (moth bean) and V. umbellata (rice bean) are much used in the tropics for forage and soil improvement, and their seeds are palatable and rich in protein. Psophocarpus tetragonolobus (winged bean) is collected in Southeast Asia for the edible fruits and protein-rich tubers. Pachyrhizus (yam…
Suppliers List, E-mail/RFQ Form, Molecular Structure, Weight, Formula, IUPAC, Synonyms for (1-OXYL-2,2,5,5-TETRAMETHYL-3-PYRROLINE-3-METHYL) METHANETHIOSULFONATE (CAS No. 81213-52-7)
Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle between P5C and proline is very important for the regulation of amino acid metabolism, intracellular redox potential, and apoptosis. Here, we present the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1 Angstroms resolution ternary complex with NAD(P)H and substrate-analog. The refined structures demonstrate a decameric architecture with five homodimer subunits and ten catalytic sites arranged around a peripheral circular groove. Mutagenesis and kinetic studies reveal the pivotal roles of the dinucleotide-binding Rossmann motif and residue Glu221 in the human enzyme. Human P5CR is thermostable and the crystals were grown at 37 degrees C. The enzyme is implicated in oxidation of the anti-tumor drug thioproline ...
Biochemistry, Metabolism:, Hereditary Factors:, Origin:, Pathology:, Strains: AKR, AU, C57BL/6, DBA/2 (212), L (P), LP, P, SIMPSON, PL (PLA, PLB), RF (W), SJL, SM, ST/B (STB), SWR, 129, RIII/S. ...
Complete information for PYCR2 gene (Protein Coding), Pyrroline-5-Carboxylate Reductase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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methyl 4-amino-5-bromopyridine-3-carboxylate; CAS Number: 1446182-20-2; Linear Formula: C7H7BRN2O2; find AChemBlock-ADVH94547B44 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
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Base de maquillaje compacta, pret à porter, ligerísima sobre la piel, uniforma el color del cutis sin recargarlo.. Su textura impalpable la hace perfecta para pieles de normales a grasas, el acabado es mate gracias a la mica y el silicio presentes en la fórmula.. La fórmula está enriquecida además por vigna aconitifolia, garcinia mangostana, aceite de macadamia, aceite de aguacate, manteca de karitè, aceite de karanja y aceite de albaricoque.. La cobertura es media/ligera, pero modulable. Por eso, es posible estratificar el producto para obtener una mayor cobertura.. ...
Fabro et al. Plant Physiol Biochem. 2020. Proline dehydrogenase (ProDH) is a flavoenzyme that catalyzes the oxidation of proline (Pro) into Δ1-pyrroline-5-carboxylate (P5C). In eukaryotes, ProDH coordinates with different Pro metabolism enzymes to control energy supply or stress responses signaling. Heterologous expression and crystallization of prokaryotic enzymes provided key data on their active center, folding capacity and oligomerization status. In contrast, eukaryotic ProDHs have not been crystallized so far, and their study as recombinant proteins remains limited. Plants contain two isoforms of ProDH with non-redundant functions. To contribute to the study of these enzymes, we describe the modeling, expression in E. coli, purification, and characterization of the Arabidopsis isoenzymes, AtProDH1 and AtProDH2. The 3D model suggested that both proteins adopt a distorted barrel structure (βα) with a cap formed by N-terminal α helices. The expression of two types of N-terminal deletion ...
Δ,sup,1,/sup,-pyrroline-5-carboxylate (P5C) reductase (P5CR) catalyses the final step of proline synthesis in plants. In Arabidopsis thaliana, protein levels are correlated neither to the corresponding mRNA copy numbers, nor to intracellular proline concentrations. The occurrence of post-translational regulatory mechanisms has therefore been hypothesized, but never assessed.,br /,,br /,The purification of A. thaliana P5CR was achieved through either a six-step protocol from cultured cells, or heterologous expression of AtP5CR in Escherichia coli. The protein was characterized with respect to structural, kinetic, and biochemical properties.,br /,,br /,P5CR was able to use either NADPH or NADH as the electron donor, with contrasting affinities and maximum reaction rates. The presence of equimolar concentrations of NADP+ completely suppressed the NADH-dependent activity, whereas the NADPH-dependent reaction was mildly affected. Proline inhibited only the NADH-dependent reaction. At physiological ...
The protein encoded by this gene is similar to proline dehydrogenase (oxidase) 1, a mitochondrial enzyme which catalyzes the first step in proline catabolism. The function of this protein has not been determined. [provided by RefSeq, Jul 2008 ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012 ...
In many plants, free proline accumulates in response to the imposition of a wide range of biotic and abiotic stresses. Controversy has surrounded the extent to which this shift in nitrogen metabolism
Amino Acids and DerivativesProline and 4-hydroxyprolineProline, 4-hydroxyproline uptake and utilization Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) ...
Methyl 2-amino-5-bromothiazole-4-carboxylate 850429-60-6 NMR spectrum, Methyl 2-amino-5-bromothiazole-4-carboxylate H-NMR spectral analysis, Methyl 2-amino-5-bromothiazole-4-carboxylate C-NMR spectral analysis ect.
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Reaction of ethyl 5-acetyl-3,4-dihydropyridine-1(2H)-carboxylate with diverse 1,3-N,N-bis-nucleophiles results in the regioselective formation of the correspondingly substituted pyrimidines in good yields.. ...
Instructions for use: Blend a small amount onto your skin from the nose outwards For a more flawless finish, pat on top of areas where extra coverage is needed using a brush 40ml/1.4 fl.oz. Ingredients: Cyclopentasiloxane, Water (Aqua), Hydrogenated Didodecene, Titanium Dioxide (Nano), Ethylhexyl Methoxycinnamate, Mica, Glycerin, Sorbitan Isostearate, Peg/Ppg 18/18 Dimethicone, Sodium Chloride, Cyclohexasiloxane , Aluminum/Magnesium Hydroxide Stearate, Dimethicone/Vinyl Dimethicone Crosspolymer, Squalane, Cetearyl Ethylhexanoate, Dimethicone, Bis-C24-28 Hydroxyalkyl Olivoyl Glutamate , Disodium Cocoyl Glutamate, Methylparaben, Potassium Sorbate, Propylparaben, Isopropyl Titanium Triisostearate, Fragrance (Parfum), Glycine Soja (Soybean) Sterols, Sodium Cocoyl Glutamate, Laureth-4, Pentaerythrityl Tetra-Di-T-Butyl Hydroxyhydrocinnamate, Sodium Citrate, Vigna Aconitifolia Seed Extract, Bht, Chlorphenesin, [May Contain: Titanium Dioxide (CI77891), Mica, Iron Oxides (CI77491 ,CI 77492, CI77499),
Instructions for use: Blend a small amount onto your skin from the nose outwards For a more flawless finish, pat on top of areas where extra coverage is needed using a brush 40ml/1.4 fl.oz. Ingredients: Cyclopentasiloxane, Water (Aqua), Hydrogenated Didodecene, Titanium Dioxide (Nano), Ethylhexyl Methoxycinnamate, Mica, Glycerin, Sorbitan Isostearate, Peg/Ppg 18/18 Dimethicone, Sodium Chloride, Cyclohexasiloxane , Aluminum/Magnesium Hydroxide Stearate, Dimethicone/Vinyl Dimethicone Crosspolymer, Squalane, Cetearyl Ethylhexanoate, Dimethicone, Bis-C24-28 Hydroxyalkyl Olivoyl Glutamate , Disodium Cocoyl Glutamate, Methylparaben, Potassium Sorbate, Propylparaben, Isopropyl Titanium Triisostearate, Fragrance (Parfum), Glycine Soja (Soybean) Sterols, Sodium Cocoyl Glutamate, Laureth-4, Pentaerythrityl Tetra-Di-T-Butyl Hydroxyhydrocinnamate, Sodium Citrate, Vigna Aconitifolia Seed Extract, Bht, Chlorphenesin, [May Contain: Titanium Dioxide (CI77891), Mica, Iron Oxides (CI77491 ,CI 77492, CI77499),
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Read Effect of Mutations in Genes PH085 and PH04 on Proline Utilization in Yeast Saccharomyces cerevisiae, Russian Journal of Genetics on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Amino Acids and DerivativesProline and 4-hydroxyprolineA Hypothetical Protein Related to Proline Metabolism Pyrroline-5-carboxylate reductase (EC 1.5.1.2) ...
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Fe2+-dependent enzyme. The enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin. It differs from EC 1.14.11.34, 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming), because it does not form guanidine and (S)-1-pyrroline-5-carboxylate from 3-hydroxy-L-arginine ...
SWISS-MODEL Template Library (SMTL) entry for 1tj2.2. Crystal structure of E. coli PutA proline dehydrogenase domain (residues 86-669) complexed with acetate
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Hydroxypethidine Hydroxypethidine Systematic (IUPAC) name Ethyl 4-(3-hydroxyphenyl)-1-methyl-piperidine-4-carboxylate Identifiers CAS number 468-56-4 ATC code
Promag P는 다양한 산업에서 가장 까다로운 요구 사항을 가진 분야에서 선호되는 센서입니다. 기초적인 어플리케이션 및 직접 통합을 위해 Promag 10 트랜스미터와 결합 된 Promag 10P는 부식성 액체 및 고온이 있는 화학 및 프로세스 응용 산업 전용입니다.
This enzyme helps to break down the pyrroline-5-carboxylate produced in the previous reaction, converting it to the amino acid ... A deficiency of either proline oxidase or pyrroline-5-carboxylate dehydrogenase results in a buildup of proline in the body. A ... This enzyme begins the process of degrading proline by starting the reaction that converts it to pyrroline-5-carboxylate. ... Pyridoxal phosphate de-activation by pyrroline-5-carboxylic acid. Increased risk of vitamin B6 deficiency and seizures in ...
Dengue fever Dennis-Cohen syndrome Dennis-Fairhurst-Moore syndrome Dent disease Dental aberrations steroid dehydrogenase ... hypertension Dextrocardia with situs inversus Dextrocardia Dextrocardia-bronchiectasis-sinusitis D-glycerate dehydrogenase ... duplication Digitorenocerebral syndrome Digoxin toxicity Dihydropteridine reductase deficiency Dihydropyrimidine dehydrogenase ... nephrogenic type 1 Diabetes insipidus, nephrogenic type 2 Diabetes insipidus, nephrogenic type 3 Diabetes insipidus, ...
In many prokaryotes, proline dehydrogenase and P5C dehydrogenase form a bifunctional enzyme that prevents the release of P5C ... The enzyme pyrroline-5-carboxylate reductase converts P5C into proline In proline degradation, the enzyme proline dehydrogenase ... "Entrez Gene: ALDH18A1 aldehyde dehydrogenase 18 family, member A1". Liu, Li-Kai; Becker, Donald F.; Tanner, John J. (2017-10-15 ... Heacock, Anne M.; Williams, Irene H.; Frank, Leonard H.; Adams, Elijah (1975-04-01). "Δ1-Pyrroline-5-carboxylate and Δ1- ...
III Delta1-Pyrroline-5-carboxylic acid dehydrogenase". J. Biol. Chem. 235: 3218-3223. Biology portal. ... L-pyrroline-5-carboxylate-NAD+ oxidoreductase, and 1-pyrroline-5-carboxylate:NAD+ oxidoreductase. This enzyme participates in ... In enzymology, a 1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88) is an enzyme that catalyzes the chemical reaction (S)-1- ... pyrroline-5-carboxylate + NAD+ + 2 H2O ⇌ {\displaystyle \rightleftharpoons } L-glutamate + NADH + H+ The three substrates of ...
1998). "Mutations in the Delta1-pyrroline 5-carboxylate dehydrogenase gene cause type II hyperprolinemia". Hum. Mol. Genet. 7 ( ... This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent ... dehydrogenase that catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to ... "Entrez Gene: ALDH4A1 aldehyde dehydrogenase 4 family, member A1". Human ALDH4A1 genome location and ALDH4A1 gene details page ...
B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, ... S)-1-pirolidin-5-karboksilat + NAD(P)+ + 2H2O ⇌. {\displaystyle \rightleftharpoons }. L-glutamat + NAD(P)H + H+. Ovaj enzim ... 1-pirolin-5-karboksilat dehidrogenaza (EC 1.5.1.12, Delta1-pirolidin-5-karboksilat dehidrogenaza, 1-pirolidinska dehidrogenaza ... Δ1-Pyrroline-5-carboxylic acid dehydrogenase". J. Biol. Chem. 235: 3218-3223.. ...
Aldehyde Dehydrogenase 18 Family, Member A1; ALDH18A1 - 138250 Reddy GP, Mishra B, Upadhyaya DN (2019). "Acquired Localized ... Pyrroline-5-Carboxylate Reductase 1; PYCR1 - 179035 Online Mendelian Inheritance in Man (OMIM): ... 8 (1): 42-51. doi:10.1159/000443696. PMC 4899661. PMID 27293393. Bouloc A, Godeau G, Zeller J, Wechsler J, Revuz J, Cosnes A ( ... 64 (1): 55-58. doi:10.4103/ijd.IJD_14_18. PMC 6340237. PMID 30745636. Rongioletti F, Cutolo M, Bondavalli P, Rebora A (January ...
In enzymology, a proline dehydrogenase (EC 1.5.5.2, formerly EC 1.5.99.8) is an enzyme that catalyzes the chemical reaction L- ... Other names in common use include L-proline dehydrogenase, and L-proline:(acceptor) oxidoreductase. This enzyme participates in ... Scarpulla RC, Soffer RL (1978). "Membrane-bound proline dehydrogenase from Escherichia coli Solubilization, purification, and ... 1-pyrroline-5-carboxylate + ubiquinol Thus, the two substrates of this enzyme are L-proline and ubiquinone, whereas its two ...
3-Hydroxybutyryl-CoA dehydrogenase. *Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase ... 29 (5): 422-8. doi:10.1007/BF02602912. PMID 2575672. S2CID 36704558.. *^ Brown JR, Masuchi Y, Robb FT, Doolittle WF (June 1994 ... 978-1-305-57720-6. .. *^ Ivanovsky RN, Khatipov EA (1994). "Evidence of covalent modification of glutamine synthetase in the ... doi:10.1016/S0197-0186(02)00033-5. PMID 12020613. S2CID 24661063.. *^ Venkatesh K, Srikanth L, Vengamma B, Chandrasekhar C, ...
3-Hydroxybutyryl-CoA dehydrogenase. *Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase ... Pyrroline-5-carboxylate reductase. *1-Pyrroline-5-carboxylate dehydrogenase/ALDH4A1. *PYCR1. arginine→. *Ornithine ... o-Quinone undergoes several reactions to eventually form melanin.[5] Tyrosinase is a copper-containing enzyme present in plant ... GeneReviews/NCBI/NIH/UW entry on Oculocutaneous Albinism Type 1. *Tyrosinase at the US National Library of Medicine Medical ...
3-Hydroxybutyryl-CoA dehydrogenase. *Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase ... Pyrroline-5-carboxylate reductase. *1-Pyrroline-5-carboxylate dehydrogenase/ALDH4A1. *PYCR1. arginine→. *Ornithine ... To date, two structures of human kynureninase have determined by X-ray diffraction with resolutions of 2.0 and 1.7 Å.[1][8] ... doi:10.1007/s00018-003-3161-1. PMID 14704851.. *. Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial ...
glycine dehydrogenase (decarboxylating) or just glycine dehydrogenase. L-protein (GCSL or DLD) EC 1.8.1.4 known by many names, ... but most commonly dihydrolipoyl dehydrogenase H-protein (GCSH) is modified with lipoic acid and interacts with all other ... doi:10.1016/S1360-1385(01)01892-1. PMID 11286922.. *^ Fujiwara K, Okamura K, Motokawa Y (Oct 1979). "Hydrogen carrier protein ... Pyrroline-5-carboxylate reductase. *1-Pyrroline-5-carboxylate dehydrogenase/ALDH4A1. *PYCR1. arginine→. *Ornithine ...
3-Hydroxybutyryl-CoA dehydrogenase. *Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase ... 47 (5): 677-81. doi:10.1002/mus.23633. PMID 23400941.. *^ O'Dwyer AM, Sheppard NP (May 1993). "The role of creatine kinase in ... 29 (1): 36-42. doi:10.1097/HJH.0b013e32834068e0. PMID 21063205.. *^ Brewster LM, Coronel CM, Sluiter W, Clark JF, van Montfrans ... 31 (3): 171-5. doi:10.1080/07435800500371706. PMID 16392619.. *^ Johannsen S, Berberich C, Metterlein T, Roth C, Reiners K, ...
PheA uses a simple dehydrogenase to convert prephenate to phenylpyruvate, while TyrA uses a NAD-dependent dehydrogenase to make ... Relevant enzymes include aspartokinase, aspartate-semialdehyde dehydrogenase, homoserine dehydrogenase, homoserine O- ... β-aspartate semialdehyde dehydrogenase, homoserine dehydrogenase, homoserine kinase, threonine synthase. ... Phosphoglycerate dehydrogenase is regulated by the concentration of serine in the cell. At high concentrations this enzyme will ...
Branched-chain alpha-keto acid dehydrogenase complex. *3-hydroxy-2-methylbutyryl-CoA dehydrogenase ... 281 (5): 272-273. doi:10.1056/NEJM196907312810518. PMID 5791298.. *^ Knowles, William S. (1983). "Asymmetric hydrogenation". ... Pyrroline-5-carboxylate reductase. *1-Pyrroline-5-carboxylate dehydrogenase/ALDH4A1. *PYCR1. arginine→. *Ornithine ... 81 (3-4): 1-21. doi:10.1080/00218460590944602.. *^ "Study Reveals Details Of Mussels' Tenacious Bonds". Science Daily. Aug 16, ...
20alpha-hydroxysteroid dehydrogenase Ja 1.1.1.153 sepiapterin reductase Ja 1.1.1.170 sterol-4alpha-carboxylate 3-dehydrogenase ... pyrroline-5-carboxylate reductase Ja 1.5.1.3 dihydrofolate reductase Ja 1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP+) ... oxoglutarate dehydrogenase (succinyl-transferring) Ja 1.2.4.4 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl- ... L-iditol 2-dehydrogenase Ja 1.1.1.15 D-iditol + NAD+ ⇌. {\displaystyle \rightleftharpoons }. D-sorbose + NADH + H+ D-iditol 2- ...
... which is reduced to proline by pyrroline-5-carboxylate reductase (using NADH or NADPH), or turned into ornithine by ornithine ... Retrieved 5 March 2018. R.H.A. Plimmer (1912) [1908], R.H.A. Plimmer & F.G. Hopkins (ed.), The chemical composition of the ... 19 (1): 37-42. doi:10.1002/cbic.201700548. PMC 5977977. PMID 29064600. Sarkar, S.K.; Young, P.E.; Sullivan, C.E. & Torchia, D.A ... 309-. ISBN 978-1-4398-4833-3. Archived from the original on 14 May 2016. Verbruggen N, Hermans C (2008). "Proline accumulation ...
For example, Birch reduction of pyrrole esters and amides produced pyrrolines, with the regioselectivity depending on the ... Structural analogs of pyrrole include: Pyrroline, a partially saturated analog with one double bond Pyrrolidine, the saturated ... which is reduced to proline by pyrroline-5-carboxylate reductase (using NADH or NADPH), or turned into ornithine by ornithine ... Pyrroles can undergo reductions to pyrrolidines and to pyrrolines. ...
... which is reduced to proline by pyrroline-5-carboxylate reductase (using NADH or NADPH), or turned into ornithine by ornithine ... Glutamate-5-semialdehyde is first formed by glutamate 5-kinase (ATP-dependent) and glutamate-5-semialdehyde dehydrogenase ( ... which requires NADH or NADPH). This can then either spontaneously cyclize to form 1-pyrroline-5-carboxylic acid, ...
Hu CA, Khalil S, Zhaorigetu S, Liu Z, Tyler M, Wan G, Valle D (Nov 2008). "Human Delta1-pyrroline-5-carboxylate synthase: ... Baumgartner MR, Rabier D, Nassogne MC, Dufier JL, Padovani JP, Kamoun P, Valle D, Saudubray JM (Jan 2005). "Delta1-pyrroline-5- ... carboxylate synthase deficiency: neurodegeneration, cataracts and connective tissue manifestations combined with ... The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo ...
Pyrroline-5-carboxylate reductase 1, mitochondrial is an enzyme that in humans is encoded by the PYCR1 gene. This gene encodes ... "Entrez Gene: PYCR1 pyrroline-5-carboxylate reductase 1". Herzfeld A, Legg MA, Greengard O (1978). "Human colon tumors: enzymic ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Pyrroline-5-carboxylate reductase ... Basch JJ, Wickham ED, Farrell HM (1997). "Pyrroline-5-carboxylate reductase in lactating bovine mammary glands". J. Dairy Sci. ...
... pyrroline carboxylate reductases MeSH D08.811.682.662.750 - saccharopine dehydrogenases MeSH D08.811.682.662.825 - ... malate dehydrogenase MeSH D08.811.682.047.748 - malate dehydrogenase (nadp+) MeSH D08.811.682.047.892 - xanthine dehydrogenase ... acetoin dehydrogenase MeSH D08.811.682.047.070 - alcohol dehydrogenase MeSH D08.811.682.047.150 - carbohydrate dehydrogenases ... acyl-coa dehydrogenase MeSH D08.811.682.660.150.150 - acyl-coa dehydrogenase, long-chain MeSH D08.811.682.660.150.200 - acyl- ...
"Crystal structures of Delta1-piperideine-2-carboxylate/Delta1-pyrroline-2-carboxylate reductase belonging to a new family of ... including glyceraldehyde 3-phosphate dehydrogenase and pyruvate dehydrogenase. In healthy mammalian tissues, estimates of the ... Rossmann fold in part of the lactate dehydrogenase of Cryptosporidium parvum, showing NAD+ in red, beta sheets in yellow, and ... Lesk AM (1995). "NAD-binding domains of dehydrogenases". Curr. Opin. Struct. Biol. 5 (6): 775-83. doi:10.1016/0959-440X(95) ...
Pyrroline-5-carboxylate is further reduced by the enzyme pyrroline-5-carboxylate reductase (P5CR) to yield a proline amino acid ... Aspartate-semialdehyde dehydrogenase catalyzes the reduction reaction by dephosphorylation of aspartyl-β-phosphate to yield ... kinase catalyzes the initial step in the diaminopimelic acid pathway by transferring a phosphoryl from ATP onto the carboxylate ... The next reaction is catalyzed by the enzyme pyrroline-5-carboxylate synthase (P5CS), which catalyzes the reduction of the ϒ- ...
"Crystal structures of Delta1-piperideine-2-carboxylate/Delta1-pyrroline-2-carboxylate reductase belonging to a new family of ... Lesk AM (1995). "NAD-binding domains of dehydrogenases". Curr. Opin. Struct. Biol. 5 (6): 775-83. doi:10.1016/0959-440X(95) ... 21 (FVII · FIX · FX · FXI · FXII · FD · PROC · Trombin) · .22 · .23 · .24 (.1 ALA · .7 MMP-1 · .17 MMP-3/MMP-6 · .19 BMP-1 · . ... Helikase · Girase · Konvertase (C3/C5) · Permease (ATPase · EEAT · FATP · GLUT · MCT · OATP · SGLT · UCP-1) · Aldolase · ...
Crystal structures of Delta1-piperideine-2-carboxylate/Delta1-pyrroline-2-carboxylate reductase belonging to a new family of ... Lesk AM «NAD-binding domains of dehydrogenases». Curr. Opin. Struct. Biol., 5, 6, 1995, pàg. 775-83. DOI: 10.1016/0959-440X(95) ... Jameson DM, Thomas V, Zhou DM «Time-resolved fluorescence studies on NADH bound to mitochondrial malate dehydrogenase». Biochim ... Biellmann JF, Lapinte C, Haid E, Weimann G «Structure of lactate dehydrogenase inhibitor generated from coenzyme». Biochemistry ...
"Crystal structures of Delta1-piperideine-2-carboxylate/Delta1-pyrroline-2-carboxylate reductase belonging to a new family of ... Lesk AM (1995). "NAD-binding domains of dehydrogenases". Curr. Opin. Struct. Biol. 5 (6): 775-83. PMID 8749365. doi:10.1016/ ... Biellmann JF, Lapinte C, Haid E, Weimann G (1979). "Structure of lactate dehydrogenase inhibitor generated from coenzyme". ... Jameson DM, Thomas V, Zhou DM (1989). "Time-resolved fluorescence studies on NADH bound to mitochondrial malate dehydrogenase ...
"Crystal structures of Delta1-piperideine-2-carboxylate/Delta1-pyrroline-2-carboxylate reductase belonging to a new family of ... including glyceraldehyde 3-phosphate dehydrogenase and pyruvate dehydrogenase. In healthy mammalian tissues, estimates of the ... Lesk AM (1995). "NAD-binding domains of dehydrogenases". Curr. Opin. Struct. Biol. 5 (6): 775-83. doi:10.1016/0959-440X(95) ... ISBN 978-0-911910-27-8. Biellmann JF, Lapinte C, Haid E, Weimann G (1979). "Structure of lactate dehydrogenase inhibitor ...
2015-09-03). "Recurrent De Novo Mutations Affecting Residue Arg138 of Pyrroline-5-Carboxylate Synthase Cause a Progeroid Form ... January 30, 2020). "Loss-of-function mutations in UDP-Glucose 6-Dehydrogenase cause recessive developmental epileptic ... ISBN 978-1-107-00382-8 - via Google Books. Rosier, Florence. "Sur la piste d'un gène responsable de la gémellité" [On the trail ... 167 (1): 187-202.E17. doi:10.1016/j.cell.2016.09.001. PMID 27662089. Zhonga, Franklin L.; Robinson, Kim; Teo, Daniel Eng Thiam ...
The change in protonation state occurs at pH 4.07.[9] This form with both carboxylates lacking protons is dominant in the ... Glutamate undergoes deamination, an oxidative reaction catalysed by glutamate dehydrogenase,[15] as follows: glutamate + H2O + ... Grabowska, A.; Nowicki, M.; Kwinta, J. (2011). "Glutamate dehydrogenase of the germinating triticale seeds: Gene expression, ... 2 (5): 225-233. doi:10.1007/BF01246639.. *^ a b c William H. Brown and Lawrence S. Brown (2008), Organic Chemistry (5th edition ...
... namely acyl-CoA dehydrogenase, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and thiolase. The cycle produces a new ... Acetyl-CoA can be carboxylated in the cytosol by acetyl-CoA carboxylase, giving rise to malonyl-CoA, a substrate required for ... The oxidative conversion of pyruvate into acetyl-CoA is referred to as the pyruvate dehydrogenase reaction. It is catalyzed by ... Acetyl-CoA serves as an allosteric regulator of pyruvate dehydrogenase kinase (PDK). It regulates through the ratio of acetyl- ...
The change in protonation state occurs at pH 4.07.[9] This form with both carboxylates lacking protons is dominant in the ... Glutamate undergoes deamination, an oxidative reaction catalysed by glutamate dehydrogenase,[15] as follows: glutamate + H2O + ... 2 (5): 225-233. doi:10.1007/BF01246639. S2CID 93590487.. *^ a b c William H. Brown and Lawrence S. Brown (2008), Organic ... 5H. 9O. 4N. Its molecular structure could be idealized as HOOC-CH(NH. 2)-(CH. 2)2-COOH, with two carboxyl groups -COOH and one ...
Glyoxylate is then oxidized by hepatic lactate dehydrogenase to oxalate in an NAD+-dependent reaction.[26] ... These can be classified into N-centered and carboxylate-center reactions. The amine undergoes the expected reactions. With acid ... Glycine (symbol Gly or G;[5] /ˈɡlaɪsiːn/)[6] is an amino acid that has a single hydrogen atom as its side chain. It is the ... 4 + N5,N10-Methylene tetrahydrofolate + NADH + H+ ⇌ Glycine + tetrahydrofolate + NAD+. Degradation[edit]. Glycine is degraded ...
The histidine-bound heme group of succinate dehydrogenase, an electron carrier in the mitochondrial electron transfer chain. ... and the favorable hydrogen bonding between a carboxylate and an NH. This should act to decrease the N1-H tautomer preference.[5 ... 193 (1): 397-404. PMID 14907727.. *^ a b Ingle, Robert A. "Histidine Biosynthesis". The Arabidopsis Book. 9: e0141. doi:10.1199 ... 60 (1): 44-69. ISSN 0146-0749. PMC 239417. PMID 8852895.. *^ a b Kulis-Horn, Robert K; Persicke, Marcus; Kalinowski, Jörn (2014 ...
The change in protonation state occurs at pH 4.07.[9] This form with both carboxylates lacking protons is dominant in the ... Glutamate undergoes deamination, an oxidative reaction catalysed by glutamate dehydrogenase,[15] as follows: glutamate + H2O + ... Grabowska, A.; Nowicki, M.; Kwinta, J. (2011). "Glutamate dehydrogenase of the germinating triticale seeds: Gene expression, ... 2 (5): 225-233. doi:10.1007/BF01246639.. *^ a b c William H. Brown and Lawrence S. Brown (2008), Organic Chemistry (5th edition ...
1,3-bisphospho-D-glycerate 3-phosphoglycerate kinase 3-phospho-D-glycerate Phosphoglyceromutase 2-phospho-D-glycerate ... Click on genes, proteins and metabolites below to link to respective articles. [§ 1] ... Carboxylate anions. *Organophosphates. *Photosynthesis. *Biochemistry stubs. Hidden categories: *Articles without InChI source ... Thus, two equivalents of 3-phosphoglycerate are produced for each molecule of CO2 that is fixed.[1][2] ...
III Delta1-Pyrroline-5-carboxylic acid dehydrogenase". J. Biol. Chem. 235: 3218-3223. Biology portal. ... L-pyrroline-5-carboxylate-NAD+ oxidoreductase, and 1-pyrroline-5-carboxylate:NAD+ oxidoreductase. This enzyme participates in ... In enzymology, a 1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88) is an enzyme that catalyzes the chemical reaction (S)-1- ... pyrroline-5-carboxylate + NAD+ + 2 H2O ⇌ {\displaystyle \rightleftharpoons } L-glutamate + NADH + H+ The three substrates of ...
... dehydrogenase (aka P5CDH and ALDH4A1) is an aldehyde dehydrogenase that catalyzes the oxidation of γ-glutamate semialdehyde to ... dehydrogenase (aka P5CDH and ALDH4A1) is an aldehyde dehydrogenase that catalyzes the oxidation of γ-glutamate semialdehyde to ... The trends in Ki likely reflect a trade-off between the penalty for desolvating the carboxylates of the free inhibitor and the ... pyrroline-5-carboxylate dehydrogenase (ALDH4A1): Semialdehyde chain length.. Pemberton, T.A., Tanner, J.J.. (2013) Arch Biochem ...
Pyrroline hydroxycarboxylic acid + NAD + Water → 4-Hydroxy-L-glutamic acid + NADH + Hydrogen Ion. details ... Pyrroline hydroxycarboxylic acid + NADP + Water → 4-Hydroxy-L-glutamic acid + NADPH + Hydrogen Ion. details ... Hempel J, Eckey R, Berie D, Romovacek H, Agarwal DP, Goedde HW: Human liver glutamic gamma-semialdehyde dehydrogenase: ... Agarwal DP, Eckey R, Hempel J, Goedde HW: Human liver high Km aldehyde dehydrogenase (ALDH4): properties and structural ...
1-Pyrroline-5-Carboxylate Dehydrogenase From Thermus With Bound Citrate ... Sodium binding site 1 out of 2 in 2iy6. Click to enlarge. Click to enlarge. Mono- and Stereo- picture of 5.0 Angstrom ... The binding sites of Sodium atom in the structure of 1-Pyrroline-5-Carboxylate Dehydrogenase From Thermus With Bound Citrate ( ... Sodium in the structure of 1-Pyrroline-5-Carboxylate Dehydrogenase From Thermus With Bound Citrate (pdb 2iy6). ...
... of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Delta-1- ... Genetic and Rare Diseases Information Center (GARD) - PO Box 8126, Gaithersburg, MD 20898-8126 - Toll-free: 1-888-205-2311 ...
Compare Anti-aldehyde dehydrogenase 5 family member A1 Antibody Products from leading suppliers on Biocompare. View ... Anti-aldehyde dehydrogenase 5 family member A1 Antibody Products. Anti-Aldehyde dehydrogenase 5 family member A1 antibodies are ... The protein may also be known as SSADH, SSDH, succinate-semialdehyde dehydrogenase, mitochondrial, and NAD(+)-dependent ... Your search returned 192 aldehyde dehydrogenase 5 family member A1 Antibodies across 28 suppliers. ...
Aldehyde dehydrogenase , Mitochondria , Nad or nadp as acceptor , Nucleotide binding , Oxidoreductase , Rossmann fold ... pyrroline-5-carboxylate dehydrogenase (P5CDH; also known as ALDH4A1), the aldehyde dehydrogenase that catalyzes the oxidation ... Categories: 1-pyrroline-5-carboxylate dehydrogenase , Human , Singh, R K , Tanner, J J , Acting on aldehyde or oxo group of ... The 2. 5-A-resolution crystal structure of HsP5CDH was determined using experimental phasing. Structures of the mutant enzymes ...
An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis. Boris R. Belitsky and ... The rocG gene of Bacillus subtilis, encoding a catabolic glutamate dehydrogenase, is transcribed by SigL (σ54)-containing RNA ... We conclude that high glutamate dehydrogenase activity was detected in strains with integrated rocG plasmids when the DAS was ... No glutamate dehydrogenase activity was detected in a sigL mutant (Table 2). ...
Pyrroline-5-carboxylate dehydrogenase References *↑ Srivastava D, Singh RK, Moxley MA, Henzl MT, Becker DF, Tanner JJ. The ... Aldehyde dehydrogenase , Mitochondria , Nad or nadp as acceptor , Nucleotide binding , Oxidoreductase , Rossmann fold ... the aldehyde dehydrogenase that catalyzes the oxidation of glutamate semialdehyde to glutamate. Here, we report the first ... pyrroline-5-carboxylate dehydrogenase (P5CDH; also known as ALDH4A1), ...
Proline dehydrogenase and Delta-1-pyrroline-5-carboxylate dehydrogenase. A, B. 1005. Geobacter sulfurreducens PCA. Mutation(s) ... the oxidation of L-proline to L-glutamate by the sequential activities of proline dehydrogenase and aldehyde dehydrogenase ... the oxidation of L-proline to L-glutamate by the sequential activities of proline dehydrogenase and aldehyde dehydrogenase ... 1,2-ETHANEDIOL. C2 H6 O2. LYCAIKOWRPUZTN-UHFFFAOYSA-N. Ligand Interaction. ...
Luiz Eduardo Baggio Savio 1 , Fernanda Cenci Vuaden, Angelo L Piato, Carla Denise Bonan, Angela T S Wyse ... 1 Laboratório de Neuroproteção e Doenças Metabólicas, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, ...
1-pyrroline-5-carboxylate dehydrogenase Feature Type. ORF , Verified Description. Delta-1-pyrroline-5-carboxylate dehydrogenase ...
Bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase. Salmonella enterica subsp. enterica serovar ... IPR005933 Delta1-pyrroline-5-COlate_DH-3. IPR029041 FAD-linked_oxidoreductase-like. IPR041349 PRODH. IPR024090 PRODH_PutA_dom_I ... Bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase. Salmonella enterica subsp. enterica serovar ... Bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase. Salmonella enterica subsp. enterica serovar ...
1998). "Mutations in the Delta1-pyrroline 5-carboxylate dehydrogenase gene cause type II hyperprolinemia". Hum. Mol. Genet. 7 ( ... This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent ... dehydrogenase that catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to ... "Entrez Gene: ALDH4A1 aldehyde dehydrogenase 4 family, member A1". Human ALDH4A1 genome location and ALDH4A1 gene details page ...
FAD-dependent proline dehydrogenase (PRODH) and NAD -dependent glutamate semialdehyde dehydrogenase (GSALDH) convert proline to ... 0 (GABA Antagonists); 3KX376GY7L (Glutamic Acid); 56-12-2 (gamma-Aminobutyric Acid); EC 1.3.8.6 (Glutaryl-CoA Dehydrogenase); ... In vitro modeling of experimental succinic semialdehyde dehydrogenase deficiency (SSADHD) using brain-derived neural stem cells ... NSCs were obtained from aldh5a1+/+ and aldh5a1-/- mice (aldh5a1 = aldehyde dehydrogenase 5a1 = SSADH). Multiple parameters were ...
Keywords: aldehyde dehydrogenase, Rossmann fold, nucleotide binding, acting on aldehyde or oxo group of donors, NAD or NADP as ... Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial. Species: Mus musculus [TaxId:10090]. Gene: Aldh4a1. ... Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial. Species: Mus musculus [TaxId:10090]. Gene: Aldh4a1. ... Description: Crystal structure of mouse 1-pyrroline-5-carboxylate dehydrogenase complexed with the product glutamate. Class: ...
Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial. Species: Mus musculus [TaxId:10090]. Gene: ADLH4a1, ... Compound: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial. Species: Mus musculus [TaxId:10090]. Gene: ADLH4a1, ... Description: Structure of mouse 1-Pyrroline-5-Carboxylate Dehydrogenase (ALDH4A1) complexed with succinate. Class: ...
... for the assay of P5C dehydrogenase comprised 50 mM Tris-HCl, 50 μM K-phosphate, 1.2 mM pyrroline 5-carboxylate and acetone ... Mezl VA, Knox WE: Properties and analisis of a stable derivative of pyrroline-5-carboxylic acid for use in metabolic studies. ... Urea and ammonia were determined enzymatically with glutamate dehydrogenase and a combination of urease-S and glutamate ... AspAT, aspartate aminotransferase; DH, dehydrogenase; Hyp, hydroxyproline; OAA, oxaloacetate; 4-OH-Glu, 4-hydroxy-L-glutamate. ...
5 causes are "rare" diseases *16 causes are "very rare" diseases *251 causes have no prevalence information. See the analysis ... Diet and exercise can prevent development of diabetes in pre-diabetics_1 *Maintaining a healthy brain may reduce dementia risk ... IDDM (1 cause) *Glucosuria (18 causes) *Hyperglycemia (94 causes) *Gestational diabetes (4 causes) *Acute hemorrhagic ... MODY syndrome, type 1 ... hyperglycemia*MODY syndrome, type 2 ... glucosuria*MODY syndrome, type III ... hyperglycemia*MODY ...
Genes related to succinate dehydrogenase were up-regulated under moderate and severe drought stress, whereas the genes encoding ... Genes encoding L-lactate dehydrogenase were decreased after drought stress relative to the control, which prevented the ... Moreover, some of the identified genes encoded proline degrading enzymes, such as proline dehydrogenase (PDH). The down- ... 1970;46:233-5.View ArticlePubMedPubMed CentralGoogle Scholar. *. Lawlor DW, Cornic G. Photosynthetic carbon assimilation and ...
3. Delta-1-pyrroline-5-carboxylate synthase. General function:. Involved in oxidoreductase activity. Specific function:. ... 1. Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial. General function:. Involved in oxidoreductase activity. ... Predicted GC-MS Spectrum - GC-MS (1 TMS) - 70eV, Positive. splash10-000i-9200000000-b09702360bf061bd64db. View in MoNA ... The conversion from L-Glutamate, an ATP- and NADPH-dependent reaction, is catalyzed by the enzyme Delta-1-pyrroline-5- ...
TBHG_02416 NAD-dependent glutamate dehydrogenase Gdh TBHG_01170 pyrroline-5-carboxylate dehydrogenase RocA TBHG_01833 glutamine ... TBHG_00234 succinate-semialdehyde dehydrogenase [NADP] dependent GabD1 TBHG_01688 succinate-semialdehyde dehydrogenase GabD2 ... succinate-semialdehyde dehydrogenase / glutarate-semialdehyde dehydrogenase [EC:1.2.1.16 1.2.1.79 1.2.1.20] K00135 gabD; ... succinate-semialdehyde dehydrogenase / glutarate-semialdehyde dehydrogenase [EC:1.2.1.16 1.2.1.79 1.2.1.20] K00265 gltB; ...
B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, ... S)-1-pirolidin-5-karboksilat + NAD(P)+ + 2H2O ⇌. {\displaystyle \rightleftharpoons }. L-glutamat + NAD(P)H + H+. Ovaj enzim ... 1-pirolin-5-karboksilat dehidrogenaza (EC 1.5.1.12, Delta1-pirolidin-5-karboksilat dehidrogenaza, 1-pirolidinska dehidrogenaza ... Δ1-Pyrroline-5-carboxylic acid dehydrogenase". J. Biol. Chem. 235: 3218-3223.. ...
Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole ...
The ALDH4A1 gene provides instructions for producing the enzyme pyrroline-5-carboxylate dehydrogenase, which is found in ... Pyrroline-5-carboxylate dehydrogenase starts the second step in the process that breaks down the protein building block (amino ... Yoshida A, Rzhetsky A, Hsu LC, Chang C. Human aldehyde dehydrogenase gene family. Eur J Biochem. 1998 Feb 1;251(3):549-57. ... A lack of pyrroline-5-carboxylate dehydrogenase function leads to decreased breakdown of proline and elevated levels of proline ...
Apurinic/Apyrimidinic Endonuclease 1/Redox Factor-1 Could Serve as a Potential Serological Biomarker for the Diagnosis and ... Mannose receptor C type 2 mediates 1,25(OH)2D3/vitamin D receptor-regulated collagen metabolism through collagen type 5, alpha ... 5.. Electrochemically Controlled RAFT Polymerization for Highly Sensitive Electrochemical Biosensing of Protein Kinase Activity ... The role of delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDh) in the Pacific white shrimp (Litopenaeus vannamei) during ...
A Bifunctional Enzyme (Δ1-Pyrroline-5-Carboxylate Synthetase) Catalyzes the First Two Steps in Proline Biosynthesis in Plants ... A Soybean Gene Encoding Δ1-Pyrroline-5-Carboxylate Reductase Was Isolated by Functional Complementation in Escherichia coli and ... The Role of Delta-1-Pyrroline-5-Carboxylate Dehydrogenase in Proline Degradation ...
The Role of Δ1-Pyrroline-5-Carboxylate Dehydrogenase in Proline Degradation Karen Deuschle, Dietmar Funck, Giuseppe Forlani, ... Phosphorylation of 1-Aminocyclopropane-1-Carboxylic Acid Synthase by MPK6, a Stress-Responsive Mitogen-Activated Protein Kinase ... Translational Regulation via 5′ mRNA Leader Sequences Revealed by Mutational Analysis of the Arabidopsis Translation Initiation ... Expression and Functional Roles of the Two Distinct NDH-1 Complexes and the Carbon Acquisition Complex NdhD3/NdhF3/CupA/Sll1735 ...
Deutch CE (1992) Oxidation of l-thiazolidine-4-carboxylate by l-proline dehydrogenase in Escherichia coli. J Gen Microbiol 138: ... Arthrobacter aurescens l-Glutamate-γ-semialdehyde dehydrogenase Paenarthrobacter aurescens l-Proline dehydrogenase PutA protein ... Both l-proline dehydrogenase and l-glutamate-γ-semialdehyde dehydrogenase (l-Δ1-pyrroline-5-carboxylate dehydrogenase) ... Deutch CE (2004) Oxidation of 3,4-dehydro-d-proline and other d-amino acid analogues by d-alanine dehydrogenase from ...
Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase Open Datasheet ... Lyophilized product should be stored at -20°C for up to 1 year. ... Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma- ...
  • The conversion from L-Glutamate, an ATP- and NADPH-dependent reaction, is catalyzed by the enzyme Delta-1-pyrroline-5-carboxylate synthetase (P5CS) (OMIM 138250 ). (hmdb.ca)
  • Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. (semanticscholar.org)
  • Isolation, characterization, and chromosomal location of a gene encoding the delta 1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana. (semanticscholar.org)
  • Removal of feedback inhibition of delta 1-pyrroline-5-carboxylate synthetase, a bifunctional enzyme catalyzing the first two steps of proline biosynthesis in plants. (semanticscholar.org)
  • delta 1-Pyrroline-5-carboxylate synthetase (P5CS) catalyzes the first two steps in proline biosynthesis in plants. (semanticscholar.org)
  • The 45-days-old seedlings of drought resistant (N-22, CR143-2-2) and susceptible rice (Oryza sativa L.) genotypes (Panidhan, Pusa-169) were subjected to osmotic stress in PEG-6000 solution of -10 and -16 bar and the relative water content (RWC), proline content, and pyrroline-5-carboxylate synthetase (P5CS) activity and its P5CS expression were studied. (bvsalud.org)
  • Δ1-pyrroline-5-carboxylate synthetase (P5CS), one of two main enzymes in proline biosynthesis pathway from glutamate precursor, has been demonstrated to play significant role in proline accumulation in plants under water stresses. (jpmb-gabit.ir)
  • Comparative Analysis of the Regulation of Expression and Structures of Two Evolutionarily Divergent Genes for Δ 1 -Pyrroline-5-Carboxylate Synthetase from Tomato. (jpmb-gabit.ir)
  • The genes encoding 1-pyrroline-5-carboxylate synthetase (P5CS), glutathione S-transferase (GST), superoxide dismutase (SOD), serine threonine-protein kinase (STK), serine threonine protein phosphatase (PSP), aldehyde dehydrogenase (AD), leucoanthocyanidin dioxygenase/anthocyanin synthase (LD/AS), HSP, MYB, and WRKY have shown upregulation in response to drought stress condition in leaf tissues. (sigmaaldrich.com)
  • Expression of the Saccharomyces cerevisiae inositol-1-phosphate synthase (INO1) gene is regulated by factors that affect phospholipid synthesis. (asm.org)
  • This protein family also includes saccharopine dehydrogenase and homospermidine synthase . (xfam.org)
  • Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. (wikipedia.org)
  • Hyperprolinemia, also known as proline oxidase deficiency , is related to schizoaffective disorder and hyperprolinemia, type ii , and has symptoms including seizures An important gene associated with Hyperprolinemia is PRODH (Proline Dehydrogenase 1), and among its related pathways/superpathways are Arginine and proline metabolism and Histidine, lysine, phenylalanine, tyrosine, proline and tryptophan catabolism . (malacards.org)
  • Mutations in the MCEE gene causes methymalonyl-Coa epimerase deficiency (MCEED), [5] a rare autosomal recessive inborn error of metabolism in amino acid metabolisms involving branched-chain amino acids valine , leucine , and isoleucine . (wikidoc.org)
  • The protein may also be known as SSADH, SSDH, succinate-semialdehyde dehydrogenase, mitochondrial, and NAD(+)-dependent succinic semialdehyde dehydrogenase. (biocompare.com)
  • This protein belongs to the aldehyde dehydrogenase family of proteins. (wikipedia.org)
  • Pyrroline-5-carboxylate dehydrogenase starts the second step in the process that breaks down the protein building block (amino acid) proline. (medlineplus.gov)
  • Analysis of its genome indicated strain TC1 has the potential to form a bifunctional PutA protein containing l -proline dehydrogenase and l- glutamate-γ-semialdehyde dehydrogenase ( l -Δ 1 -pyrroline-5-carboxylate dehydrogenase) activities. (springer.com)
  • Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification. (semanticscholar.org)
  • The eta isoform of protein kinase C mediates transcriptional activation of the human transglutaminase 1 gene. (naver.com)
  • We have recently identified ferritin as a cellular protein particle whose synthesis is stimulated in mouse or human cells infected by the picornavirus Mengo (1). (naver.com)
  • [1] The OAT protein is localized to the mitochondrion within the cells where it is expressed. (wikidoc.org)
  • The co-factor of the OAT protein ( pyridoxal-5'-phosphate ) binds to OAT through a Schiff base at the lysine 292 position situated between two of the seven-stranded beta-sheet. (wikidoc.org)
  • The protein encoded by this gene is similar to proline dehydrogenase (oxidase) 1, a mitochondrial enzyme which catalyzes the first step in proline catabolism. (nih.gov)
  • This family also includes omega crystallin, an eye lens protein from squid and octopus that has little aldehyde dehydrogenase activity. (umbc.edu)
  • In molecular biology , the protein domain Saccharopine dehydrogenase (SDH), also named Saccharopine reductase, is an enzyme involved in the metabolism of the amino acid lysine , via an intermediate substance called saccharopine . (xfam.org)
  • Succinate-semialdehyde dehydrogenase EC:1.2.1.16. (umbc.edu)
  • The rocG gene of Bacillus subtilis , encoding a catabolic glutamate dehydrogenase, is transcribed by SigL (σ 54 )-containing RNA polymerase and requires for its expression RocR, a member of the NtrC/NifA family of proteins that bind to enhancer-like elements, called upstream activating sequences (UAS). (pnas.org)
  • In the present work, we show that the B. subtilis rocG gene, encoding a major catabolic glutamate dehydrogenase (GlutDH) ( 17 ), is a member of the RocR regulon. (pnas.org)
  • Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the ALDH4A1 gene. (wikipedia.org)
  • The ALDH4A1 gene provides instructions for producing the enzyme pyrroline-5-carboxylate dehydrogenase, which is found in tissues throughout the body. (medlineplus.gov)
  • ALDH4A1 gene variants reduce or eliminate the function of the pyrroline-5-carboxylate dehydrogenase enzyme. (medlineplus.gov)
  • Sophos NA, Vasiliou V. Aldehyde dehydrogenase gene superfamily: the 2002 update. (medlineplus.gov)
  • The ALDH4A1 gene is found on chromosome 1 . (medlineplus.gov)
  • Proline biosynthesis in Saccharomyces cerevisiae: analysis of the PRO3 gene, which encodes delta 1-pyrroline-5-carboxylate reductase. (semanticscholar.org)
  • Molecular cloning and evidence for osmoregulation of the delta 1-pyrroline-5-carboxylate reductase (proC) gene in pea (Pisum sativum L. (semanticscholar.org)
  • 5'-flanking sequence required for regulated expression of a muscle-specific Drosophila melanogaster actin gene. (asm.org)
  • We found that the antisense overlapping gene pair of D 1-pyrroline-5-carboxylate dehydrogenase (P5CDH), a stress-related gene, and SRO5, a gene of unknown function, generates two types of siRNAs. (psu.edu)
  • Proline dehydrogenase/proline oxidase (PRODH/POX) catalyzes the first step in proline catabolism, and its function in tumors has attracted attention since it was reported to be a P53-induced gene in tumor cell lines ( 9 , 10 ). (spandidos-publications.com)
  • These mutations were secondarily screened for their effects in trans on the promoter of the PUT2 (delta 1-pyrroline-5-carboxylate dehydrogenase) gene by using a PUT2-lacZ (E. coli beta-galactosidase) gene fusion. (asm.org)
  • The theory that operons are the remnants of tandem duplication has recently been criticized by Lawrence and Roth [ 5 ], who instead proposed that horizontal gene transfer may be the underlying mechanism for the occurrence of gene clusters. (biomedcentral.com)
  • For this purpose, we investigated ( 1 ) the effect of glucagon on enzymes involved in L-hydroxyproline metabolism (experiment 1), and ( 2 ) the effect of glucagon induction of mitochondrial SPT/AGT on oxalate formation from L-hydroxyproline and that from glycolate in vivo (experiment 2). (asnjournals.org)
  • Enzymes involved in the catabolism of proline with high activities were: proline dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, fumarase, NADP-linked malic enzyme, alanine aminotransferase and malate dehydrogenase. (ac.ke)
  • We find a strong over-representation of homologous enzymes at MPL 1. (biomedcentral.com)
  • Proline metabolism is of critical importance for tumor cells, and pyrroline‑5‑carboxylate reductase 1 (PYCR1), a key proline biosynthesis enzyme, has been reported to be overexpressed in prostate cancer and to promote tumor cell growth in breast cancer. (spandidos-publications.com)
  • Pyrroline-5-carboxylate reductase (PYCR) catalyzes the last step of proline synthesis, and three isozymes are encoded by three human genes. (spandidos-publications.com)
  • P5CR, D 1 -pyrroline-5-carboxylate reductase. (sinica.edu.tw)
  • In pathway II, proline racemase (prdF) forms D-proline, and a reductase forms 5-aminovalerate. (lbl.gov)
  • The enzyme Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase (aka P5CDH and ALDH4A1) is an aldehyde dehydrogenase that catalyzes the oxidation of γ-glutamate semialdehyde to l-glutamate. (rcsb.org)
  • PutA (proline utilization A) is a large bifunctional flavoenzyme with proline dehydrogenase (PRODH) and δ 1 -pyrroline- 5-carboxylate dehydrogenase (P5CDH) domains that catalyze the oxidation of L-proline to L-glutamate in two successive reactions. (nebraska.edu)
  • In the P5CDH active site, L-glutamate- semialdehyde (the hydrolyzed form ofδ 1 -pyrroline- 5-carboxylate) undergoes a two-electron oxidation in which a hydride is transferred toNAD+-producingNADHand glutamate. (nebraska.edu)
  • A lack of pyrroline-5-carboxylate dehydrogenase function leads to decreased breakdown of proline and elevated levels of proline and intermediate breakdown product pyrroline-5-carboxylate, causing the signs and symptoms of hyperprolinemia type II. (medlineplus.gov)
  • There are two inherited forms: hyperprolinemia type 1 and hyperprolinemia type 2. (malacards.org)
  • Hyperprolinemia type 2 causes proline levels in the blood to be 10 to 15 times higher than normal, and it also causes high levels of a related compound called pyrroline-5-carboxylate. (cdc.gov)
  • Hyperprolinemia type II results in proline levels in the blood between 10 and 15 times higher than normal, and high levels of a related compound called pyrroline-5-carboxylate. (malacards.org)
  • NADP, also known as NADP+ or TPN, belongs to the class of organic compounds known as (5'->5')-dinucleotides. (ymdb.ca)
  • Proline utilization A (PutA) proteins are bifunctional peripheral membrane flavoenzymes that catalyze the oxidation of L-proline to L-glutamate by the sequential activities of proline dehydrogenase and aldehyde dehydrogenase domains. (rcsb.org)
  • Comment: In pathway I, proline dehydrogenase (put1) forms (S)-1-pyrroline-5-carboxylate, which spontaneously hydrates to L-glutamate 5-semialdehyde, and a dehydrogenase (putA) to glutamate. (lbl.gov)
  • A candidate enzyme responsible for the transamination of glyoxylate to glycine is a dual-functional enzyme, serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT, alternatively named alanine:glyoxylate aminotransferase-1, AGT-1 or serine:pyruvate aminotransferase, SPT). (asnjournals.org)
  • a dehydrogenase forms (2R)-2-amino-4-oxopentanoate, and a thiolase cleaves it to D-alanine and acetyl-CoA. (lbl.gov)
  • The systematic name of this enzyme class is (S)-1-pyrroline-5-carboxylate:NAD+ oxidoreductase. (wikipedia.org)
  • Other names in common use include delta-1-pyrroline-5-carboxylate dehydrogenase, 1-pyrroline dehydrogenase, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylic acid dehydrogenase, L-pyrroline-5-carboxylate-NAD+ oxidoreductase, and 1-pyrroline-5-carboxylate:NAD+ oxidoreductase. (wikipedia.org)
  • Purification and characterization of human liver "high Km" aldehyde dehydrogenase and its identification as glutamic gamma-semialdehyde dehydrogenase. (reactome.org)
  • This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase that catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. (wikipedia.org)
  • Amino-terminal fragments of delta 1-pyrroline-5-carboxylate dehydrogenase direct beta-galactosidase to the mitochondrial matrix in Saccharomyces cerevisiae. (asm.org)
  • also known as ALDH4A1), the aldehyde dehydrogenase that catalyzes the oxidation of glutamate semialdehyde to glutamate. (proteopedia.org)
  • PDBe-KB provides an overview of all the structure information available in the PDB for Human Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (ALDH4A1) PDBe-KB provides an overview of all the structure information available in the PDB for Mouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (ALDH4A1) Valle D, Goodman SI, Harris SC, Phang JM (1980). (wikipedia.org)
  • Mitochondrial delta-1-pyrroline-5-carboxylate dehydrogenase (ALDH4A1) catalyzes the reaction of L-glutamate gamma-semialdehyde and NAD+ to form glutamate and NADH + H+ (Hu et al. (reactome.org)
  • Mannose receptor C type 2 mediates 1,25(OH) 2 D 3 /vitamin D receptor-regulated collagen metabolism through collagen type 5, alpha 2 chain and matrix metalloproteinase 13 in murine MC3T3-E1 cells. (nih.gov)
  • In enzymology, a 1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88) is an enzyme that catalyzes the chemical reaction (S)-1-pyrroline-5-carboxylate + NAD+ + 2 H2O ⇌ {\displaystyle \rightleftharpoons } L-glutamate + NADH + H+ The three substrates of this enzyme are (S)-1-pyrroline-5-carboxylate, NAD+, and H2O, whereas its three products are glutamate, NADH, and H+. (wikipedia.org)
  • RocR controls two operons, rocABC and rocDEF , involved in arginine degradation to glutamate (Fig. 1 ). (pnas.org)
  • Or as part of L-arginine degradation I, the aminotransferase rocD converts ornithine to glutamate 5-semialdehyde, which spontaneously converts to 1-pyrroline-5-carboxylate. (lbl.gov)
  • Or 1-pyrroline-5-carboxylate can be reduced to proline by PRO3, as in L-arginine degradation VI. (lbl.gov)
  • GABA can also be produced via glutamate degradation by the glutamate decarboxylase, this variant of the pathway includes a 2-oxoglutarate-dependent 4-aminobutyrate transaminase and an NAD+-dependent dehydrogenase. (smpdb.ca)
  • For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. (lbl.gov)
  • Proline is converted to glutamate via Δ 1 -pyrroline-5-carboxylate. (pnas.org)
  • In the PRODH active site, proline undergoes a two-electron oxidation to δ 1 -pyrroline-5- carboxlylate, and the FAD cofactor is reduced. (nebraska.edu)
  • The reaction requires pyridoxal 5'-phosphate as a co-factor and forms part of the subpathway that synthesizes L- glutamate 5-semialdehyde from L- ornithine . (wikidoc.org)
  • Glutamate-5-semialdehyde dehydrogenase EC:1.2.1.41. (umbc.edu)
  • Pro dehydrogenase (PDH) catalyzes the first and rate-limiting step in the Pro catabolic pathway. (plantphysiol.org)
  • Pathway IX is similar to pathway VIII but the transaminase aruH forms the 5-guanidino-2-oxopentanoate. (lbl.gov)
  • It can be succinylated by aruFG and then catabolized by the later steps of the arginine succinyltransferase pathway, via aminotransferase, dehydrogenase, and desuccinylase reactions (see PMC179677 and PMID:7523119 ). (lbl.gov)
  • An enzyme that catalyzes the oxidation of 1-pyrroline-5-carboxylate to L-GLUTAMATE in the presence of NAD. (bvsalud.org)
  • RocG activity (glutamate + NAD → 2-ketoglutarate + NH 3 + NADH) can be viewed as the final step in the use of arginine, ornithine, and proline as carbon or nitrogen sources (Fig. 1 ). (pnas.org)
  • RocG, glutamate dehydrogenase. (pnas.org)
  • Glycine thus formed may be oxidized to CO 2 and ammonia by the mitochondrial glycine cleavage enzyme system, and in conjugation with this oxidation another molecule of glycine is converted to L-serine by mitochondrial serine hydroxymethyltransferase ( 5 ). (asnjournals.org)
  • [5] Tyrosinase is a copper -containing enzyme present in plant and animal tissues that catalyzes the production of melanin and other pigments from tyrosine by oxidation. (wikipedia.org)
  • Enhancer elements and enhancer-binding proteins are widespread components of transcriptional regulation systems in eukaryotes ( 1 ). (pnas.org)
  • To initiate transcription, σ 54 -containing RNA polymerases must interact with additional proteins, members of the NtrC/NifA family, that bind to UAS usually located 100-200 bp upstream of the regulated genes ( 2 - 5 ). (pnas.org)
  • It has been shown that UAS can be moved 1-2 kilobases (kb) further upstream from the regulated genes without losing their ability to interact with UAS-binding proteins and to activate expression of the regulated genes ( 6 , 7 ). (pnas.org)
  • Forty-five different proteins were further analysed, corresponding to (1) spots stained with more than four of 13 (30 %) sera tested with the CCLP1 or the CCSW1 cell line and with the normal liver, and (2) to spots immunoreactive with at least two of the five sera probed with their tumour and non-tumour counter-part of cholangiocarcinoma. (biomedcentral.com)
  • the saposin-like proteins 1, 2, and 3 of fasciola gigantica. (liverpool.ac.uk)
  • This step converts pyrroline-5-carboxylate, which is produced in the first step, to the amino acid glutamate. (medlineplus.gov)
  • A dehydrogenase converts this to glutamate. (lbl.gov)
  • Converts proline to delta-1-pyrroline-5-carboxylate. (nih.gov)
  • In most cases, they are the upstream activating sequences (UAS) required for expression of genes transcribed by RNA polymerases containing σ 54 (RpoN)-like subunits ( 2 - 5 ). (pnas.org)
  • Eukaryotic aldehyde dehydrogenase (ALDH) genes: human polymorphisms, and recommended nomenclature based on divergent evolution and chromosomal mapping. (medlineplus.gov)
  • Glutamate semialdehyde is spontaneously converted to pyrroline 5-carboxylate, the more-stable cyclic form of glutamate semialdehyde. (asmscience.org)
  • Activities of 1-pyrroline-5 carboxylate dehydrogenase, glutamate dehydrogenase, aspartate aminotransferase and NAD-linked malic enzyme were detectable but lower. (ac.ke)
  • Both l -proline dehydrogenase and l- glutamate-γ-semialdehyde dehydrogenase ( l -Δ 1 -pyrroline-5-carboxylate dehydrogenase) activities were found primarily in a membrane fraction from exponential-phase cells. (springer.com)
  • Methylmalonate-semialdehyde dehydrogenase EC:1.2.1.27. (umbc.edu)
  • Vasiliou V, Pappa A. Polymorphisms of human aldehyde dehydrogenases. (medlineplus.gov)
  • The family includes the following members: The prototypical members are the aldehyde dehydrogenases EC:1.2.1.3. (umbc.edu)
  • [1] Several natural variants in amino acid sequences exist. (wikidoc.org)
  • Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. (hmdb.ca)
  • Catalytic transfer of hydride ions from NADPH to oxygen by the interconversions of proline and delta 1-pyrroline-5-carboxylate. (semanticscholar.org)
  • Transfer of reducing equivalents into mitochondria by the interconversions of proline and delta 1-pyrroline-5-carboxylate. (semanticscholar.org)
  • Efficient 4- and 5-carbon substrates share the common feature of being long enough to span the distance between the anchor loop at the bottom of the active site and the oxyanion hole at the top of the active site. (rcsb.org)
  • This family of dehydrogenases act on aldehyde substrates. (umbc.edu)
  • Although At-PDH has been referred to as Pro oxidase, its classification as a dehydrogenase is more appropriate (N. Verbruggen, unpublished results). (plantphysiol.org)
  • Microdeletions of chromosome 22q11 are associated with variable phenotypic expression that often includes learning disabilities, palatal abnormalities, congenital heart defects, and mildly dysmorphic facial features ( 1 ). (pnas.org)
  • The utilization of carbon-1 compounds by plants. (deepdyve.com)
  • RESULTS: Au -MPO is a potent TGR inhibitor that achieves 75% inhibition at a 1:1 TGR:Au ratio and efficiently kills E. granulosus in vitro. (bireme.br)
  • [5] Mutations in this domain are correlated with hereditary diseases . (wikidoc.org)
  • For example, because of its colocalization with ornithine decarboxylase (ODC) in the cytosol, arginase I may preferentially direct ornithine to polyamine synthesis, whereas arginase II may preferentially direct ornithine to proline and glutamate production due to the colocalization of arginase II and ornithine aminotransferase (OAT) in mitochondria (Fig. 1 ). (physiology.org)