A large family of transmembrane proteins found in TIGHT JUNCTIONS. They take part in the formation of paracellular barriers and pores that regulate paracellular permeability.
A ubiquitously-expressed claudin subtype that acts as a general barrier-forming protein in TIGHT JUNCTIONS. Elevated expression of claudin-3 is found in a variety of tumor cell types, suggesting its role as a therapeutic target for specific ANTINEOPLASTIC AGENTS.
An integral membrane protein that is localized to TIGHT JUNCTIONS, where it plays a role in controlling the paracellular permeability of polarized cells. Mutations in the gene for claudin-1 are associated with Neonatal Ichthyosis-Sclerosing Cholangitis (NISCH) Syndrome.
A claudin subtype that takes part in maintaining the barrier-forming property of TIGHT JUNCTIONS. Claudin-4 is found associated with CLAUDIN-8 in the KIDNEY COLLECTING DUCT where it may play a role in paracellular chloride ion reabsorption.
Cell-cell junctions that seal adjacent epithelial cells together, preventing the passage of most dissolved molecules from one side of the epithelial sheet to the other. (Alberts et al., Molecular Biology of the Cell, 2nd ed, p22)
A claudin subtype that is found localized to TIGHT JUNCTIONS in VASCULAR ENDOTHELIAL CELLS. The protein was initially identified as one of several proteins which are deleted in VELOCARDIOFACIAL SYNDROME and may play an important role in maintaining the integrity of the BLOOD-BRAIN BARRIER.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A MARVEL domain protein that plays an important role in the formation and regulation of the TIGHT JUNCTION paracellular permeability barrier.
A 195-kDa zonula occludens protein that is distinguished by the presence of a ZU5 domain at the C-terminal of the molecule.
A zonula occludens protein subtype found in epithelial cell junctions. Several isoforms of zonula occludens-2 protein exist due to use of alternative promoter regions and alternative mRNA splicings.
A claudin subtype that is associated with the formation of cation-selective channels and increased epithelial permeability. It is localized to the TIGHT JUNCTIONS of the PROXIMAL KIDNEY TUBULE and INTESTINAL EPITHELIUM.
A specialized barrier, in the TESTIS, between the interstitial BLOOD compartment and the adluminal compartment of the SEMINIFEROUS TUBULES. The barrier is formed by layers of cells from the VASCULAR ENDOTHELIUM of the capillary BLOOD VESSELS, to the SEMINIFEROUS EPITHELIUM of the seminiferous tubules. TIGHT JUNCTIONS form between adjacent SERTOLI CELLS, as well as between the ENDOTHELIAL CELLS.
Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.
The resistance to the flow of either alternating or direct electrical current.
The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.
A genus of pufferfish commonly used for research.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Excretion of abnormally high level of CALCIUM in the URINE, greater than 4 mg/kg/day.
An inherited renal disorder characterized by defective NaCl reabsorption in the convoluted DISTAL KIDNEY TUBULE leading to HYPOKALEMIA. In contrast with BARTTER SYNDROME, Gitelman syndrome includes hypomagnesemia and normocalcemic hypocalciuria, and is caused by mutations in the thiazide-sensitive SODIUM-POTASSIUM-CHLORIDE SYMPORTERS.
A condition characterized by calcification of the renal tissue itself. It is usually seen in distal RENAL TUBULAR ACIDOSIS with calcium deposition in the DISTAL KIDNEY TUBULES and the surrounding interstitium. Nephrocalcinosis causes RENAL INSUFFICIENCY.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.
The U-shaped portion of the renal tubule in the KIDNEY MEDULLA, consisting of a descending limb and an ascending limb. It is situated between the PROXIMAL KIDNEY TUBULE and the DISTAL KIDNEY TUBULE.
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Cells of epithelial origin possessing specialized sensory functions. They include cells that are found in the TASTE BUDS; OLFACTORY MUCOSA; COCHLEA; and NEUROEPITHELIAL BODIES.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.
Supporting cells projecting inward from the basement membrane of SEMINIFEROUS TUBULES. They surround and nourish the developing male germ cells and secrete ANDROGEN-BINDING PROTEIN and hormones such as ANTI-MULLERIAN HORMONE. The tight junctions of Sertoli cells with the SPERMATOGONIA and SPERMATOCYTES provide a BLOOD-TESTIS BARRIER.
A usually benign glandular tumor composed of oxyphil cells, large cells with small irregular nuclei and dense acidophilic granules due to the presence of abundant MITOCHONDRIA. Oxyphil cells, also known as oncocytes, are found in oncocytomas of the kidney, salivary glands, and endocrine glands. In the thyroid gland, oxyphil cells are known as Hurthle cells and Askanazy cells.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)
Established cell cultures that have the potential to propagate indefinitely.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Degree of saltiness, which is largely the OSMOLAR CONCENTRATION of SODIUM CHLORIDE plus any other SALTS present. It is an ecological factor of considerable importance, influencing the types of organisms that live in an ENVIRONMENT.
The simultaneous analysis of multiple samples of TISSUES or CELLS from BIOPSY or in vitro culture that have been arranged in an array format on slides or microchips.
The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A general term for the complete or partial loss of the ability to hear from one or both ears.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH.
Phenotypic changes of EPITHELIAL CELLS to MESENCHYME type, which increase cell mobility critical in many developmental processes such as NEURAL TUBE development. NEOPLASM METASTASIS and DISEASE PROGRESSION may also induce this transition.
The portion of renal tubule that begins from the enlarged segment of the ascending limb of the LOOP OF HENLE. It reenters the KIDNEY CORTEX and forms the convoluted segments of the distal tubule.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.
Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.
A malignant neoplasm arising from tenosynovial tissue of the joints and in synovial cells of tendons and bursae. The legs are the most common site, but the tumor can occur in the abdominal wall and other trunk muscles. There are two recognized types: the monophasic (characterized by sheaths of monotonous spindle cells) and the biphasic (characterized by slit-like spaces or clefts within the tumor, lined by cuboidal or tall columnar epithelial cells). These sarcomas occur most commonly in the second and fourth decades of life. (From Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1363)
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The mucous membrane lining the RESPIRATORY TRACT, including the NASAL CAVITY; the LARYNX; the TRACHEA; and the BRONCHI tree. The respiratory mucosa consists of various types of epithelial cells ranging from ciliated columnar to simple squamous, mucous GOBLET CELLS, and glands containing both mucous and serous cells.
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
An EPITHELIUM with MUCUS-secreting cells, such as GOBLET CELLS. It forms the lining of many body cavities, such as the DIGESTIVE TRACT, the RESPIRATORY TRACT, and the reproductive tract. Mucosa, rich in blood and lymph vessels, comprises an inner epithelium, a middle layer (lamina propria) of loose CONNECTIVE TISSUE, and an outer layer (muscularis mucosae) of SMOOTH MUSCLE CELLS that separates the mucosa from submucosa.
Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The sequential location of genes on a chromosome.
Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
A cell line derived from cultured tumor cells.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.
Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Refers to animals in the period of time just after birth.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
The relationships of groups of organisms as reflected by their genetic makeup.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
MAMMARY GLANDS in the non-human MAMMALS.
A nonparametric method of compiling LIFE TABLES or survival tables. It combines calculated probabilities of survival and estimates to allow for observations occurring beyond a measurement threshold, which are assumed to occur randomly. Time intervals are defined as ending each time an event occurs and are therefore unequal. (From Last, A Dictionary of Epidemiology, 1995)
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Tumors or cancer of the human BREAST.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
Adherence of cells to surfaces or to other cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Laboratory tests used to evaluate how well the kidneys are working through examination of blood and urine.
Proteins prepared by recombinant DNA technology.
Claudin, P; Andreotti, B (2006). "A scaling law for aeolian dunes on Mars, Venus, Earth, and for subaqueous ripples". Earth and ... Chang, Kenneth (October 1, 2013). "Hitting Pay Dirt on Mars". New York Times. Retrieved October 2, 2013. Meslin, P.-Y.; Forni, ... 252 (1-2): 30-44. arXiv:cond-mat/0603656. Bibcode:2006E&PSL.252...30C. doi:10.1016/j.epsl.2006.09.004. S2CID 13910286. Sullivan ... 186 (1): 60-96. Bibcode:2007Icar..186...60C. doi:10.1016/j.icarus.2006.08.019. ...
Bordiec, M.; Carpy, S.; Bourgeois, O.; Herny, C.; Massé, M.; Perret, L.; Claudin, P.; Pochat, S.; Douté, S. (1 December 2020 ... Only about 1% of Antarctic ice area can be considered to be blue-ice area, but they have attracted scientific interest due to ... Blue-ice areas make up only about 1% of the Antarctic surface ice; however, they are locally common and scattered across the ... doi:10.1111/j.1945-5100.2001.tb01918.x. Harvey, Ralph (1 January 2003). "The Origin and Significance of Antarctic Meteorites". ...
Claudin, Philippe; Andreotti, Bruno (2006). "A scaling law for aeolian dunes on Mars, Venus, Earth, and for subaqueous ripples ... 252 (1-2): 30-44. arXiv:cond-mat/0603656. doi:10.1016/j.epsl.2006.09.004. ISSN 0012-821X. Williams, S. H.; Zimbelman, J. R.; ... 1 m in amplitude. Understanding TAR formation and evolution could offer insight into the winds that created them. In turn, ... 31 (1): e18-e18. doi:10.1130/0091-7613-31.1.e18. ISSN 1943-2682. Wolfe, Stephen A.; Huntley, David J.; Ollerhead, Jeff (2006-07 ...
Claudin, A. (1894). Les Origines de l'imprimerie à Saint-Lô en Normandie [The origins of printing at Saint-Lô in Normandy] (in ... French). Paris: A. Claudin. Le Clerc, R. (1930). Histoire du Bon Sauveur de Saint-Lô [History of the Bon Sauveur of Saint-Lô] ( ... It is also chef-lieu of an arrondissement and two cantons (Saint-Lô-1 and Saint-Lô-2). The placename derives from that of a ... Messier 1 April 1771 made at the Observatory of Saint-Lô, in Rouen] (in French). Paris: Imprimerie Royale. Mourier, Guillaume ( ...
February 2018). "claudin 5". Biology Open. 7 (2): bio030494. doi:10.1242/bio.030494. PMC 5861362. PMID 29437557. Abbott NJ, ... 8 (1): 7. doi:10.1186/2045-8118-8-7. PMC 3042981. PMID 21349155. Chen Y, Imai H, Ito A, Saito N (2013). "Novel modified method ... 34 (1): 147-52. doi:10.1111/j.1471-4159.1980.tb04633.x. PMID 7452231. Raza MW, Shad A, Pedler SJ, Karamat KA (March 2005). " ... 80 (1): 32-9. doi:10.1124/mol.111.071027. PMID 21454448. S2CID 7146614. Krol S, Macrez R, Docagne F, Defer G, Laurent S, Rahman ...
Van Itallie CM, Mitic LL, Anderson JM (July 2012). "SUMOylation of claudin-2". Annals of the New York Academy of Sciences. 1258 ... 374 (Pt 1): 1-20. doi:10.1042/BJ20030407. PMC 1223585. PMID 12773095. Liu B, Gross M, ten Hoeve J, Shuai K (March 2001). "A ... 554 (1-2): 111-8. doi:10.1016/s0014-5793(03)01116-5. PMID 14596924. S2CID 23261716. Wong KA, Kim R, Christofk H, Gao J, Lawson ... 229 (1-2): 109-16. doi:10.1016/s0378-1119(99)00033-5. PMID 10095110. Betz A, Lampen N, Martinek S, Young MW, Darnell JE (August ...
Claudin-12 is a protein that in humans is encoded by the CLDN12 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN12 claudin 12". Human CLDN12 genome location and CLDN12 gene details page in the UCSC Genome Browser. Kniesel ... 2001). "claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung- and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-20 is a protein that in humans is encoded by the CLDN20 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN20 claudin 20". CS1 maint: discouraged parameter (link) Human CLDN20 genome location and CLDN20 gene details ... Hewitt KJ, Agarwal R, Morin PJ (Aug 2006). "The claudin gene family: expression in normal and neoplastic tissues". BMC Cancer. ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin 4, also known as CLDN4, is a protein which in humans is encoded by the CLDN4 gene. It belongs to the group of claudins ... "Entrez Gene: CLDN4 claudin 4". Human CLDN4 genome location and CLDN4 gene details page in the UCSC Genome Browser. Kniesel U, ... This gene encodes an integral membrane protein, which belongs to the claudin family. The protein is a component of tight ... 2003). "Claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer". Cancer Res. 63 (19): 6265- ...
Claudin-14 is a protein that in humans is encoded by the CLDN14 gene. It belongs to a related family of proteins called ... "Entrez Gene: CLDN14 claudin 14". Baker M, Reynolds LE, Robinson SD, Lees DM, Parsons M, Elia G, et al. (2013). "Stromal ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Sticky cells, blood vessels and cancer - the paradox of Claudin-14 - Marianne Baker, Cancer Research UK Science Update blog, 14 ...
Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins. Claudins, such as ... "Entrez Gene: CLDN7 claudin 7". CS1 maint: discouraged parameter (link) Human CLDN7 genome location and CLDN7 gene details page ... 2005). "Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study". Mod. Pathol. 18 (4 ... 2003). "Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and ...
2007). "Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus". J. Virol. 81 (22): 12465-71. doi: ... Claudin-9 is a protein that in humans is encoded by the CLDN9 gene. It belongs to the group of claudins. This gene is involved ... "Entrez Gene: CLDN9 claudin 9". Gene discovery reveals a critical protein's function in hearing Human CLDN9 genome location and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. It belongs to the group of claudins. Tight junctions ... "Entrez Gene: CLDN1 claudin 1". Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (Nov 2003). "Role of claudin ... The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight ... Miyamori H, Takino T, Kobayashi Y, Tokai H, Itoh Y, Seiki M, Sato H (2001). "Claudin promotes activation of pro-matrix ...
Claudin-8 is a protein that in humans is encoded by the CLDN8 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN8 claudin 8". CS1 maint: discouraged parameter (link) Human CLDN8 genome location and CLDN8 gene details page ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Morita K, Furuse M, Fujimoto K, Tsukita S (Mar 1999). "Claudin multigene family encoding four-transmembrane domain protein ...
Claudin-22 is a protein that in humans is encoded by the CLDN22 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN22 claudin 22". CS1 maint: discouraged parameter (link) Human CLDN22 genome location and CLDN22 gene details ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Heiskala M, Peterson PA, Yang Y (2001). "The roles of claudin superfamily proteins in paracellular transport". Traffic. 2 (2): ...
Claudin-16 is a protein that in humans is encoded by the CLDN16 gene. It belongs to the group of claudins. Tight junctions ... The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight ... 2004). "A Novel Claudin 16 Mutation Associated with Childhood Hypercalciuria Abolishes Binding to ZO-1 and Results in Lysosomal ... "Entrez Gene: CLDN16 claudin 16". "Salmonella infection data for Cldn16". Wellcome Trust Sanger Institute. "Citrobacter ...
Jia, P.; B. Andreotti; P. Claudin (March 2017). "Giant ripples on comet 67P/Churyumov-Gerasimenko sculpted by sunset thermal ... Hayes, A.G. (1 June 2018). "Dunes across the Solar System". Science. 360 (6392): 960-961. Bibcode:2018Sci...360..960H. doi: ... 1 June 2018). "Dunes on Pluto". Science. 360 (6392): 992-997. Bibcode:2018Sci...360..992T. doi:10.1126/science.aao2975. PMID ... spaced about 0.4 to 1 km apart, that are thought to be composed of 200-300 μm diameter particles of methane ice believed to be ...
Claudin domain-containing protein 1 is a protein that in humans is encoded by the CLDND1 gene. GRCh38: Ensembl release 89: ... "Entrez Gene: CLDND1 claudin domain containing 1". Human CLDND1 genome location and CLDND1 gene details page in the UCSC Genome ... 289 (1-2): 119-29. doi:10.1016/S0378-1119(02)00507-3. PMID 12036590. Zhang QH, Ye M, Wu XY, et al. (2001). "Cloning and ...
"Claudin-1 overexpression effect on lung adenocarcinoma cell line". NCBI GEO Profiles. Retrieved 4 May 2015. Vandepoele K, Van ... Neuroblastoma breakpoint family, member 1, or NBPF1, is a protein that is encoded by the gene NBPF1 in humans. This protein is ... The NBPF1 protein is also found to be disrupted by a chromosomal translocation between chromosomes 1 and 17 with in some cases ... Additionally, the inactivation of Far upstream element-binding protein 1 causes a decrease in NBPF1, while the inactivation of ...
Claudin-17 is a protein that in humans is encoded by the CLDN17 gene. It belongs to the group of claudins. It forms anion- ... "Entrez Gene: CLDN17 claudin 17". Krug SM, Günzel D, Conrad MP, Rosenthal R, Fromm A, Amasheh S, Schulzke JD, Fromm M (2012). " ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Heiskala M, Peterson PA, Yang Y (2001). "The roles of claudin superfamily proteins in paracellular transport". Traffic. 2 (2): ...
Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. It belongs to the group of claudins. This gene encodes a ... "Entrez Gene: CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome)". Coyne CB, Gambling TM, Boucher RC, ... Kojima S, Rahner C, Peng S, Rizzolo LJ (2002). "Claudin 5 is transiently expressed during the development of the retinal ... Tsukita S, Furuse M (2002). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-11 is a protein that in humans is encoded by the CLDN11 gene. It belongs to the group of claudins and was the first ... "Entrez Gene: CLDN11 claudin 11 (oligodendrocyte transmembrane protein)". Gow A, Southwood CM, Li JS, Pariali M, Riordan GP, ... The protein encoded by this gene belongs to the claudin family of tight junction associated proteins and is a major component ... Tiwari-Woodruff SK, Buznikov AG, Vu TQ, Micevych PE, Chen K, Kornblum HI, Bronstein JM (April 2001). "OSP/claudin-11 forms a ...
Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. It is a member of the claudin ... "Entrez Gene: CLDN3 claudin 3". Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (Nov 2003). "Role of claudin ... "Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium". Cancer Res ... The protein encoded by this intron-less gene, a member of the claudin family, is an integral membrane protein and a component ...
"Entrez Gene: CLDN18 claudin 18". CS1 maint: discouraged parameter (link) Niimi T, Nagashima K, Ward JM, et al. (2001). "claudin ... Claudin 18.2) is abundant in gastric tumors. Experimental antibody IMAB362 targets Claudin 18.2 to help treat gastric cancers. ... Claudin-18 is a protein that in humans is encoded by the CLDN18 gene. It belongs to the group of claudins. CLDN18 belongs to ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-19 is a protein that in humans is encoded by the CLDN19 gene. It belongs to the group of claudins. Claudin-19 has been ... "Entrez Gene: CLDN19 claudin 19". Naeem, M.; Hussain, S.; Akhtar, N. (2011). "Mutation in the Tight-Junction Gene Claudin 19 ( ... 2006). "Kidney claudin-19: localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease". ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-15 is a protein that in humans is encoded by the CLDN15 gene. It belongs to the group of claudins. Among its related ... "Entrez Gene: CLDN15 claudin 15". Database, GeneCards Human Gene. "CLDN15 Gene - GeneCards , CLD15 Protein , CLD15 Antibody". ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Heiskala M, Peterson PA, Yang Y (2001). "The roles of claudin superfamily proteins in paracellular transport". Traffic. 2 (2): ...
Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. It belongs to the group of claudins. Members of the claudin ... Claudin-2 is expressed in cation-leaky epithelia such as that of the kidney proximal tubule. Mice that are deficient in claudin ... "Entrez Gene: CLDN2 claudin 2". CS1 maint: discouraged parameter (link) Muto, S.; Hata, M.; Taniguchi, J.; Tsuruoka, S.; ... 1998). "Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to ...
This mechanism is mediated by host claudin-3 and claudin-4 receptors, situated at the tight junctions. Clostridium enterotoxin ... "Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium". Cancer ... This allows the human claudin-3,4,6,7,8 and 14 to bind but not 1,2,5, and 10. The way the protein work is it destroys the cell ... Van Itallie CM, Betts L, Smedley JG, McClane BA, Anderson JM (January 2008). "Structure of the claudin-binding domain of ...
"Tradition and Imitation in Pierre Certon's Déploration for Claudin de Sermisy". Revue de Musicologie. 85 (1): 29-62. doi: ... ISBN 978-1-5017-0486-4. Reese, Gustave (1940). Music in the Middle Ages: With an Introduction on the Music of Ancient Times. ... ISBN 978-1-137-27770-1. Wilkins, Nigel (1979). Music in the age of Chaucer. Cambridge, England: D.S. Brewer. ISBN 978-0-85991- ... ISBN 978-1-84383-016-0. Leach, Elizabeth Eva (2014). Guillaume de Machaut: Secretary, Poet, Musician. Ithaca, New York: Cornell ...
Claudin, F., Ernstson, K., Rampino, M.R., and Anguita, F. 2001. Striae, polish, imprints, rotated fractures, and related ... Ernstson, K., Claudin, F., Schüssler, U., Anguita, F. and Ernstson, T. 2001. Impact melt rocks, shock metamorphism, and ... Missing or empty ,title= (help) Ernstson, K., Claudin, F., Schüssler, U. & Hradil, K. (2002): The mid-Tertiary Azuara and ... and Claudin, F. 1994. Shock cratering of conglomeratic quartzite pebbles and the search and identification of an Azuara (Spain ...
While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the ... Often, several claudin family members are coexpressed and interact with each other, and this determines the overall ... IPR006187. Claudin. IPR003548. Claudin1. IPR017974. Claudin_CS. IPR004031. PMP22/EMP/MP20/Claudin. ... IPR006187. Claudin. IPR003548. Claudin1. IPR017974. Claudin_CS. IPR004031. PMP22/EMP/MP20/Claudin. ...
Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed ... Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed ... Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed ... Claudin-1 Target. Polyclonal Antibody - Claudin-1 Antibody - Immunoprecipitation, Western Blotting, UniProt ID O95832, Entrez ...
Using FRET analysis, the proximity of claudin N- and C-termini integrated in homopolymeric strands composed of claudin-3 or of ... The results indicate at least two different cis-interaction interfaces within claudin-3 homopolymers as well as within claudin- ... FRET; cell junctions; claudin; claudin strand model; live-cell imaging; tight junction architecture ... Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK ( ...
... and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of ... claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial ... α/claudin-1 axis regulates barrier dysfunction in eosinophilic esophagitis. ... α/claudin-1 axis regulates barrier dysfunction in eosinophilic esophagitis. ...
Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice. J Cell ... Heatmaps generated from normalized data (n = 3). (E) Western blotting confirmed claudin-1 protein loss in HIF1A-KD and HIF1A-DN ... We first noted that normal human esophageal biopsies possess the primary claudin genes CLDN1, CLDN4, and CLDN7 (Figure 3C). We ... B) Mice were assessed for claudin-1 protein by Western blot, quantified by densitometry, and CLDN1 mRNA by real-time RT-PCR. (C ...
... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ...
2018 Dec 15;373(1-2):44-56. doi: 10.1016/j.yexcr.2018.08.012. Epub 2018 Aug 9. Research Support, Non-U.S. Govt ... TUSC3 accelerates cancer growth and induces epithelial-mesenchymal transition by upregulating claudin-1 in non-small-cell lung ... On the contrary, overexpression of TUSC3 significantly enhanced EMT progress by upregulating claudin-1 expression. Overall, our ... In addition, TUSC3 knockdown suppressed epithelial-mesenchymal transition by downregulating the expression of claudin-1, which ...
Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression ... In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant ... Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant ... We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon ...
... a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are… ... Recombinant protein of human claudin 1 (CLDN1). Human. > 80 % Preparation: Recombint protein was captured through anti-DDK ... Background of Claudin-1 / CLDN1 antibody. Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and ... Western blot (WB) analysis of Claudin-1 antibody (Cat.-No.: AP06060PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2: ...
Claudin-1 / CLDN1, 50 µg. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets ... Claudin-1 / CLDN1. Not available. Human > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column ... Positive controls for Claudin-1 / CLDN1 (3 products). Catalog No.. Species. Pres.. Purity. Source. ... Proteins for Claudin-1 / CLDN1 (3 products). Catalog No.. Species. Pres.. Purity. Source. ...
GST-claudin-6 tail, GST-claudin-9 tail, GST-claudin-1 tail(YV/AA), GST-claudin-6 tail(YV/AA), or GST-claudin-9 tail(YV/AA). ... Claudin-1 was transported from the ER in an in vitro budding assay. Huh7.5 cells were treated with digitonin to prepare the ... C) Cells surface claudin-1 was quantified by FACS assay in nonpermeabilized cells. Huh7.5 cells treated with 1.562 μg/ml XN or ... Claudin-1 utilizes the YV motif-Sec24C interaction to exit from the ER to the Golgi compartment and eventually reach its final ...
In this study, claudin 1 promotes migration in luminal-like MCF7 human breast cancer cells and increases their sensitivity to ... Claudin 1 promotes migration and increases sensitivity to tamoxifen and anticancer drugs in luminal-like human breast cancer ... Downregulation of claudin 1, a critical tight junction protein, has been correlated with increased invasiveness in breast ... Collectively, our results suggest that claudin 1 has the potential to be used as a predictive marker for treatment efficacy for ...
A Claudin-1-Targeted HTA. Mayo Yamashita, Manami Iida, Minoru Tada, Yoshitaka Shirasago, Masayoshi Fukasawa, Shotaro Nagase, ... A Claudin-1-Targeted HTA. Mayo Yamashita, Manami Iida, Minoru Tada, Yoshitaka Shirasago, Masayoshi Fukasawa, Shotaro Nagase, ... Discovery of Anti-Claudin-1 Antibodies as Candidate Therapeutics against Hepatitis C Virus. Mayo Yamashita, Manami Iida, Minoru ... Claudin-1 (CLDN1), a known host factor for hepatitis C virus (HCV) entry and cell-to-cell transmission, is a target molecule ...
Claudin 1 (8G1) Monoclonal Antibody. Catalog Number. Pack Size. List Price*. Quantity. ... Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.. ...
... occludin and claudin-5 rearrangement and lessened IgG and Evans blue extravasation. (A) Occludin, ZO-1 and claudin-5 expression ... claudin-5 was continuously located along ECs, and fewer gaps were formed in the metformin-treated group (Figure 4A). Gap ... claudin-5 was continuously located along ECs, and fewer gaps were formed in the metformin-treated group (Figure 4A). Gap ... occludin and claudin-5 rearrangement and lessened IgG and Evans blue extravasation. (A) Occludin, ZO-1 and claudin-5 expression ...
Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. ... Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. ... Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. ... Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLDN1 (NP_066924.1). ...
... claudin 4, and claudin 7 in colorectal cancer and its relation with CLDN DNA methylation patterns. Hahn-Strömberg, Victoria ... Altered claudin expression has been described in colon, prostatic, ovarian, and breast carcinoma. However, the role of ... Tight junction, methylation, colon cancer, claudin Nationell ämneskategori Cancer och onkologi Identifikatorer. URN: urn:nbn:se ... We aimed our study to investigate whether claudin protein expression and methylation of CLDN can influence the tumorigenesis of ...
Claudin 7 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), ... Claudin 7 Antibody 1 Publications. Rabbit Polyclonal, Catalog number: 10118-1-AP ... Claudin 7 antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). ... Claudin 7 antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). ...
Claudin 18 antibody Mouse Monoclonal from Proteintech validated in Immunoprecipitation (IP), Immunohistochemistry (IHC), Enzyme ... Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight ... Claudin 18 Antibody 1 Publications. Mouse Monoclonal, Catalog number: 66167-1-Ig ,CloneNo.: 5G7F2 ... IP Result of anti-Claudin 18 (IP:66167-1-Ig, 3ug; Detection:66167-1-Ig 1:300) with mouse stomach tissue lysate 4000ug. ...
We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin-1 promoter. ... The transcription factors Slug and Snail act as repressors of Claudin-1 expression in epithelial cells1. Ofelia M. Martínez- ... Moreover, an inverse correlation in the levels of Claudin-1 and Slug transcripts were observed in breast cancer cell lines. E- ... Claudin-1 is an integral membrane protein component of tight junctions. The Snail family of transcription factors are ...
Rabbit Anti-Claudin 1 Polyclonal Antibody, Purified Rabbit Polyclonal Antibody (Pab) validated in WB, IF, IHC-P (AP52052), ... home , Products , Primary Antibodies , Rabbit Anti-Claudin 1 Polyclonal Antibody Rabbit Anti-Claudin 1 Polyclonal Antibody. ... While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the ... Often, several claudin family members are coexpressed and interact with each other, and this determines the overall ...
Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans ... Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans ... Download PDF Human epidermal Langerhans cells express the tight junction protein claudin-1 and are present in human genetic ... Human epidermal Langerhans cells express the tight junction protein claudin-1 and are present in human genetic claudin-1 ...
Effects of claudin-2, occludin or ZO-1 siRNA transfection on transepithelial electrical resistance (TER) in HK-2 cell ... claudin -2 expressiontubule epithelial cell line HK -2.claudin -2ZORNAHK -2 cellscontrol HK -2 monolayersTJ protein expression ... claudin -2 expressiontubule epithelial cell line HK -2.claudin -2ZORNAHK -2 cellscontrol HK -2 monolayersTJ protein expression ... Effects of claudin-2, occludin or ZO-1 siRNA transfection on transepithelial electrical resistance (TER) in HK-2 cell ...
CLAUDIN-1, EXPRESSION, CD81, RECEPTOR, IN-VIVO, CELL ENTRY, HUMAN LIVER, ENTRY FACTORS, HUMAN HEPATOCYTES, NEUTRALIZING ... Using a human liver-chimeric mouse model(6), we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) ... Using a human liver-chimeric mouse model(6), we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) ... Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody. Laurent ...
a) Immunohistochemistry of claudin-1 in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy ... b) Immunoblotting of claudin-1 in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each ... b) Immunoblotting of claudin-1 in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each ... Claudin-1 was most intense in the superficial layer of the vaginal epithelium in the control group. Expression of ZO-1, ...
We further provide direct evidence for the binding of the NFκB subunit p65 to the GLI1 promoter in both EMT and claudin-low ... We observed non-canonical activation of GLI1 in claudin-low and EMT cell lines, and identified crosstalk with the NFκB pathway ... Knockdown of GLI1 expression in claudin-low cell lines resulted in reduced cell viability, motility, clonogenicity, self- ... High GLI1 expression is a property of claudin-low cells and tumors and correlates with markers of EMT and breast cancer stem ...
The gene encoding claudin-1 (CLDN1) has been mapped to human chromosome 3 (HSA3; 3q28→q29) using a radiation hybrid panel. ... The gene encoding claudin-1 (CLDN1) has been mapped to human chromosome 3 (HSA3; 3q28→q29) using a radiation hybrid panel. ... Schempp, W und Schmid, M (2005) Comparative mapping of human claudin-1 (CLDN1) in great apes. Cytogenetic and Genome Research ...
... even though claudin 4 did not significantly correlate with patient age. Claudin 1 knockdown in BT-20 cells resulted in ... Although the expression of one of these proteins, claudin 1, is down regulated in most invasive human breast cancers (HBC), we ... In these tumors, the claudin 1 protein, usually localized in the cell membrane, is often mislocalized to the cytoplasm. To ... The association of high claudin 1 protein levels observed in tumors derived from older women with BLBC, suggests that claudin 1 ...
The claudin gene family: expression in normal and neoplastic tissues. BMC Cancer. 2006;6:186.PubMedCentralPubMedCrossRefGoogle ... Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals. Gene. 2011;481:83-92.PubMedCrossRef ... Claudin-18 is an early-stage marker of pancreatic carcinogenesis. J Histochem Cytochem. 2011;59:942-952.PubMedCentralPubMed ... miR-1303 Claudin-18 Stomach neoplasms Proliferation Invasion This is a preview of subscription content, log in to check access. ...
Claudin-19. A, C. 185. Mus musculus. Mutation(s): 3 Gene Names: Cldn19. ... The claudin-19/C-CPE complex shows no density of a short extracellular helix that is critical for claudins to assemble into TJ ... Here we present the structure of mammalian claudin-19 in complex with C-CPE at 3.7 Å resolution. The structure shows that C-CPE ... CRYSTAL STRUCTURE of MOUSE CLAUDIN-19 IN COMPLEX with C-TERMINAL FRAGMENT OF CLOSTRIDIUM PERFRINGENS ENTEROTOXIN. *DOI: 10.2210 ...
  • Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). (cellsignal.com)
  • The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. (cellsignal.com)
  • Probing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3. (nih.gov)
  • Cell lysates from Huh7.5.1 cells were incubated with purified GST-claudin-1 tail or GST, and proteins were pulled down with glutathione-Sepharose beads. (asm.org)
  • Candidate claudin-1 tail-interacting proteins were the GST-claudin-1-tail interactome. (asm.org)
  • Claudin-low breast cancers are characterized by low expression levels of cell-cell adhesion molecules including E-cadherin and several of the tight junction claudin proteins, claudin 3, 4, and 7. (biomedcentral.com)
  • Although the expression of one of these proteins, claudin 1, is down regulated in most invasive human breast cancers (HBC), we have recently shown that high levels of claudin 1, characterized tumors belonging to the very aggressive basal-like breast cancer (BLBC) subtype. (biomedcentral.com)
  • As well, the exact combination of claudin proteins within a given tissue determines the selectivity, strength and tightness of the TJ [ 11 ]. (biomedcentral.com)
  • Several classes of claudin interact with other proteins to form tight junction and regulate permeability of TJs. (biomedcentral.com)
  • It is also observed that the expression of claudin proteins found to be differentially regulated in several cancers. (biomedcentral.com)
  • Most of the claudins (claudin-12 being the exception) have a C-terminal PDZ-binding motif that can interact with other PDZ domain proteins, such as scaffolding protein, ZO-1, -2 and -3 [ PMID: 24665401 ]. (ebi.ac.uk)
  • The transmembrane domain proteins of the claudin superfamily are the major structural components of cellular tight junctions. (chalmers.se)
  • We recently demonstrated the loss of functional integrity of the BTB in testicular intraepithelial neoplasia (TIN), associated with a dislocation of the peripheral TJ proteins ZO-1 and ZO-2. (nih.gov)
  • To this end, MDCK C7 cells were stably transfected with either claudin-2 or claudin-10b, two paracellular cation-channel-forming TJ proteins that are not endogenously expressed in this cell line. (biologists.org)
  • Perturbation was overexpression of either claudin-2 or claudin-10b, as both form cation channels within the TJ of MDCK C7 cells, a tight renal epithelial cell line that lacks endogenous expression of these TJ proteins. (biologists.org)
  • Molecular architecture of tight junction has been studied extensively, which has confirmed that claudin family of proteins is integral component of tight junction. (hindawi.com)
  • however, the role of claudin family of proteins play in a series of pathophysiological events, including human carcinoma development, is only now beginning to be understood. (hindawi.com)
  • Gh, prolactin and cortisol on mRNA expression of three tight junction proteins were examined: claudin-10e (Cldn-10e), Cldn-30 and zonula occludens-1 (Zo-1). (genscript.com)
  • Claudin 7 belongs to a family of 24 tight junction (TJ) membrane proteins which are central for the regulation of cell permeability and the maintenance of cell polarity. (perkinelmer.com)
  • A closer examination of isolated tight junctions uncovered two related ~22 kDa, four-transmembrane domain proteins, claudin-1 and claudin-2, with no similarity to occludin. (thermofisher.com)
  • Excitement in the tight junction field continues to rise following the recent discovery of claudins -3, -4, -5, -6, -7, and -8 and experiments suggesting that tight junctions in different tissues are comprised of different sets of claudin family proteins. (thermofisher.com)
  • In MC3T3-E1 cells but not MLO-Y4 cells, mRNAs of claudin-1, -2, and -6, Cx43, and type I collagen, and proteins of claudin-1 and Cx43 were increased after treatment with IGF-I. Such treatment significantly decreased paracellular permeability in MC3T3-E1 cells. (elsevier.com)
  • Specific role of tight junction proteins claudin-5, occludin, and ZO-1 of the blood-brain barrier in a focal cerebral ischemic insult. (qxmd.com)
  • This study was performed to determine whether BK opens the BTB by affecting the tight junction (TJ)-associated proteins zonula occluden-1 (ZO-1), occludin, and caludin-5 and cytoskeleton protein filamentous actin (F-actin). (qxmd.com)
  • Clone REA751 recognizes the human claudin-3 antigen, an integral membrane protein which is a component of the claudin family of epithelial tight junction proteins. (miltenyibiotec.com)
  • Recent investigations have focused on tight junction proteins, belonging to the claudin family (claudins-1 to -27 in humans) and the newly defined group of TAMP (three proteins: occludin, Marvel-D2, and tricellulin). (wiley.com)
  • By contrast, in MDCK I cells, PMA treatment resulted in redistribution of claudins 1, 3, 4 and 5 from the TJ and in reorganization of the proteins into more insoluble complexes. (deepdyve.com)
  • Recent evidence indicates that both exposure to HIV-1 and HIV-1 specific proteins, such as Tat protein, can contribute to alterations of expression and distribution of claudin-5 in brain endothelial cells and brain microvessels. (elsevier.com)
  • Recent work points toward the claudin family of tight junction proteins as leading candidates for the molecular components that regulate paracellular permeability properties in epithelial tissues. (uab.edu)
  • Our results show that claudin-6 protein is significantly down-regulated in breast invasive ductal carcinomas and is an important correlate with lymphatic metastasis, but claudin-6 down-regulation was not correlated with upregulation of the methylation associated proteins (MeCP2, DNMT1) or histone modification associated proteins (HDAC1, H3Ac, H4Ac). (biomedcentral.com)
  • These proteins, such as claudin-5, occludin, and zona occludens 1 (ZO-1) prevent transcellular diffusion of molecules and cells ( 1 ). (frontiersin.org)
  • Other tight junction proteins, zonula occludens (ZO)-1, ZO-2, claudin-2, and claudin-3, were also decreased in polyamine-deficient cells. (physiology.org)
  • Major transmembrane and cytosolic tight junction proteins in the mammalian epithelium include occludin, claudins, zonula occludens (ZO)-1, and ZO-2 ( 8 , 12 , 41 , 54 , 66 ). (physiology.org)
  • Several transmembrane adhesive proteins have been identified which include vascular endothelial (VE) and neural (N) cadherin at adherens junctions (AJs), 6,7 occludin, 8 and members of the claudin family, 9 as well as the junctional adhesion molecule (JAM) family at tight junctions (TJs). (ahajournals.org)
  • 10,11 PECAM-1 12,13 or S-endo-1/Muc 18/CD146 14 are proteins that are not components of the major junctional systems but localize along the intercellular cleft. (ahajournals.org)
  • As the first step in understanding the physiologic functions of claudins (tight junction integral membrane proteins) in nephrons, the expression of claudin-1 to -16 in mouse kidneys was examined by Northern blotting. (asnjournals.org)
  • The main results are that (i) within homo- and heteropolymers, the average distance between the cytoplasmic ends of the TM1s of cis-interacting claudin molecules is shorter than the average distance between their TM4s, and (ii) TM1 segments of neighbouring claudins are oriented towards each other as the cytoplasmic end of TM1 is in close proximity to more other TM1 segments than TM4 is to other TM4 segments. (nih.gov)
  • In normal mucosa, cytoplasm showed no staining for claudins in any patient, whereas in paired colorectal cancer tissues, significant cytoplasmic staining appeared both for claudin 1 (p = 0.04) and claudin 4 (p = 0.01). (diva-portal.org)
  • The claudin-19/C-CPE complex shows no density of a short extracellular helix that is critical for claudins to assemble into TJ strands. (rcsb.org)
  • In humans, 24 claudins (claudin 1-24) have been identified. (ebi.ac.uk)
  • Claudins are the backbone of tight junction strands, and are essential components to the function of tight junctions [1]. (oatext.com)
  • It is through those "other" functions [1,2] that TJs and especially claudins play a role in carcinogenesis. (oatext.com)
  • A prime example of the aforementioned role of claudins in carcinogenesis and metastases is claudin-1. (oatext.com)
  • Claudin-1 along with claudin-3 and claudin-5 are shown to promote pro-MMP2 whereby claudins recruit MMPs on the cell surface to achieve elevated focal concentrations and eventual activations of pro-MMP2 [12]. (oatext.com)
  • Several claudin mouse knockout models have been generated and the diversity of phenotypes observed clearly demonstrates their important roles in the maintenance of tissue integrity in various organs and suggest that claudins also participate in cellular contexts other than tight junctions. (hindawi.com)
  • Claudin-4 is the first to confer ionic selectivity to paracellular transport, leading to the prediction that the combination of different claudins defines the overall selectivity of different junctions. (thermofisher.com)
  • However, there is little knowledge about the relationship of ASK1 and claudins, especially claudin-6. (biomedcentral.com)
  • Unlike in MDCK II cells, induction of claudin-8 in MDCK I cells (which did not affect levels of endogenous claudins) did not alter paracellular ion permeability. (deepdyve.com)
  • Our findings demonstrate that claudin overexpression studies measure the combined effect of alterations in both endogenous and exogenous claudins, thus explaining the dependence of the phenotype on the host cell line. (deepdyve.com)
  • In HT29 cells, claudins 1, 3, 4 and 5 and possibly claudin 2 were redistributed to apical cell-cell contacts after PKC activation and the amounts of claudins 1, 3 and 5, but not of claudin 2, were increased in cell lysates. (deepdyve.com)
  • Claudins 1 and 4 were phosphorylated in both MDCK I and HT29 cells, but PKC-induced changes in claudin phosphorylation state were detected only in MDCK I cells. (deepdyve.com)
  • When each claudin species or occludin was overexpressed in mouse L fibroblasts, claudin molecules, but not occludin, were polymerized within the plasma membranes to reconstitute paired TJ strands, indicating that claudins are major structural components of TJ strands ( 13 ). (asnjournals.org)
  • Here, we identified a regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively controlling tight junction CLDN1 (claudin-1). (jci.org)
  • Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. (acris-antibodies.com)
  • Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. (acris-antibodies.com)
  • Western Blot analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: Non-transfected lysate. (acris-antibodies.com)
  • WB Suggested Anti-CLDN1 Antibody Titration: 0.2-1 ug/ml. (acris-antibodies.com)
  • Western Blot analysis of CLDN1 expression in transfected 293T cell line ( H00009076-T02 ) by CLDN1 MaxPab polyclonal antibody.Lane 1: CLDN1 transfected lysate(22.70 KDa).Lane 2: Non-transfected lysate. (acris-antibodies.com)
  • Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. (lsbio.com)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLDN1 (NP_066924.1). (lsbio.com)
  • In conclusion, our results show that CLDN1 is significantly hypomethylated in tumor samples and that the membrane staining intensity for claudin 1, 4, and 7 is significantly lower in colorectal cancer tissues than in adjacent nonneoplastic tissue. (diva-portal.org)
  • The nucleotide sequence data reported for Canis familiaris partial mRNA for putative claudin-1 protein ( cldn1 gene) has been submitted to DDBJ, EMBL, GenBank® and GSDB Nucleotide Sequence Databases under the accession number AJ628857 . (biochemj.org)
  • Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans cells in the epidermis. (uzh.ch)
  • The tight junction protein claudin-1 (CLDN1) has been shown to be required for entry of HCV into the cell. (ox.ac.uk)
  • Three host cell molecules have been reported to be important entry factors or receptors for HCV internalization: scavenger receptor BI, the tetraspanin CD81, and the tight junction protein claudin-1 (CLDN1). (ox.ac.uk)
  • The tight junction protein claudin-1 (CLDN1) has been shown to be essential for hepatitis C virus (HCV) entry-the first step of viral infection. (bham.ac.uk)
  • In this study, we show that CLDN1 has a causal role in the epithelial-mesenchymal transition (EMT) in human liver cells, and that the c-Abl-Ras-Raf-1-ERK1/2 signaling axis is critical for the induction of malignant progression by CLDN1. (ajou.ac.kr)
  • Collectively, these results indicate that CLDN1 is necessary for the induction of EMT in human liver cells, and that activation of the c-Abl-Ras-Raf-1-ERK1/2 signaling pathway is required for CLDN1-induced acquisition of the malignant phenotype. (ajou.ac.kr)
  • Using an iterative expression cloning approach we identified claudin-1 (CLDN1), a tight junction component that is highly expressed in the liver, as essential for HCV entry. (ox.ac.uk)
  • Unexpectedly, the anti-claudin-1 antibody localized claudin to the Golgi apparatus of a sub-population of SA and RA in addition to the distal junctions of RA. (frontiersin.org)
  • Immunohistochemistry (IHC) analyzes of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • Western blot (WB) analysis of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • Final antibody concentration is 1 mg/ml. (acris-antibodies.com)
  • The lysates were immunoprecipitated with anti-claudin-1 antibody followed by Western blotting. (asm.org)
  • Claudin 1 (8G1) Monoclonal Antibody by Bioss, Cat. (lucerna-chem.ch)
  • Using a human liver-chimeric mouse model(6), we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) eliminates chronic HCV infection without detectable toxicity. (ugent.be)
  • Claudin-9 Polyclonal antibody specifically detects Claudin-9 in Human samples. (fishersci.com)
  • We offer Claudin-14 Antibody Pairs for use in common research applications: Immunoprecipitation, Western Blot. (novusbio.com)
  • Each Claudin-14 Antibody Pair is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Our Claudin-14 Antibody Pairs can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our Claudin-14 Antibody Pairs. (novusbio.com)
  • Mice with PU.1 deficiency in T cells were protected from colitis, whereas treatment with antibody to IL-9 suppressed colitis. (nature.com)
  • The following product was used in this experiment: Claudin 5 Monoclonal Antibody (4C3C2) from Thermo Fisher Scientific, catalog # 35-2500, RRID AB_2533200. (thermofisher.com)
  • Recent antibody-based studies indicated that Claudin 7 is not only localized at the apical TJs but also have a strong basolateral membrane distribution in the epithelia of various tissues. (perkinelmer.com)
  • This antibody reacts specifically with the ~ 22-24 kDa endogenous Claudin-5 protein. (thermofisher.com)
  • IHC-P analysis of human colon tissue using GTX31496 Claudin 4 antibody. (genetex.com)
  • WB analysis of HeLa cell lysate using GTX31496 Claudin 4 antibody. (genetex.com)
  • Claudin 19 antibody LS-C503440 is an AP-conjugated mouse monoclonal antibody to human Claudin 19 (CLDN19). (lsbio.com)
  • The modulating agents comprise at least one claudin cell adhesion recognition sequence or an antibody or fragment thereof that specifically binds the claudin cell adhesion recognition sequence. (justia.com)
  • The present invention relates generally to methods for regulating claudin-mediated processes, and more particularly to the use of modulating agents comprising a claudin cell adhesion recognition sequence and/or an antibody that specifically recognizes such a sequence for inhibiting functions such as cell adhesion and the formation of tissue permeability barriers. (justia.com)
  • While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. (uniprot.org)
  • Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. (uniprot.org)
  • To evaluate endothelial cell permeability after metformin treatment, we conducted occludin/CD31, ZO-1/CD31 and claudin-5/CD31 double staining to observe tight junction distribution in situ at 3 days after tMCAO. (nih.gov)
  • The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility. (ebi.ac.uk)
  • This review aims to summarise research on permeability properties of the claudin-10 channels and their role in physiology and pathophysiology of the kidney. (nih.gov)
  • Under all conditions, water flux in claudin-2-transfected cells was elevated compared with vector controls, indicating claudin-2-mediated paracellular water permeability. (biologists.org)
  • These findings suggest that the induction of tight-junction protein claudin-1 and paracellular permeability during the differentiation of osteoblast-like MC3T3-E1 cells after treatment with IGF-I is regulated via a MAP-kinase pathway, but not with respect to gap junctions. (elsevier.com)
  • Exposure of bEND3 monolayer to OGD for 2 h significantly increased its permeability to FITC-labeled dextran and promoted the secretion of metalloproteinase-2 and -9 (MMP-2/9) and cytosolic translocation of caveolin-1 (Cav-1). (qxmd.com)
  • Caveolin-1 regulates nitric oxide-mediated matrix metalloproteinases activity and blood-brain barrier permeability in focal cerebral ischemia and reperfusion injury. (qxmd.com)
  • This study demonstrates that the increase of BK-mediated BTB permeability is associated with the down-regulation of ZO-1, occludin, and claudin-5 and the rearrangement of F-actin and that cAMP/PKA signal transduction system might be involved in the modulating process. (qxmd.com)
  • Members of the claudin family are known to determine tight junction permeability in a charge- and size-selective manner. (rupress.org)
  • We previously reported that claudin-8 reduces paracellular cation permeability when expressed in low-resistance Madin-Darby canine kidney (MDCK) II cells. (deepdyve.com)
  • Furthermore, when endogenous claudin-2 in MDCK II cells was downregulated by epidermal growth factor to create a cell model with low transepithelial resistance and low levels of claudin-2, the permeability effects of claudin-8 were also abolished. (deepdyve.com)
  • BBB permeability can be altered by several factors including inflammatory molecules such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), C-C motif chemokine receptor-2 ligand (CCL-2), and interleukin-17A (IL-17A) ( 1 ). (frontiersin.org)
  • IL-1β contributes to BBB permeability in three major ways. (frontiersin.org)
  • Second, IL-1β induces expression of hypoxia-inducible factor-1α (HIF-1α) and VEGF-A, contributing to BBB permeability and increased angiogenesis ( 4 ). (frontiersin.org)
  • Third, secreted IL-1β also alters the location of CXCL12 expression in CNS endothelial cells from the basolateral BBB membrane to the luminal surface, contributing to BBB permeability to leukocytes ( 6 ). (frontiersin.org)
  • Distal junction area of ruffle-ended ameloblasts (RA) and the Golgi apparatus of a sub-population of smooth-ended ameloblasts (SA) and RAs stained positive with anti-claudin-1 antibodies. (frontiersin.org)
  • B) Huh7.5 cells treated with Sec24C siRNA or a control siRNA for 72 h were stained with antibodies against claudin-1 (green) and the tight junction marker ZO-1 (red). (asm.org)
  • A) Huh7.5 cells treated with different concentrations of XN for 12 h were fixed with methanol and stained with antibodies against claudin-1 (red) and the tight junction marker ZO-1 (green). (asm.org)
  • Both yeast membrane-bound and detergent-extracted, purified claudin-1 were antigenic and recognized by specific antibodies. (chalmers.se)
  • Monoclonal anti-claudin 1 antibodies prevent hepatitis C virus infection of primary human hepatocytes. (ox.ac.uk)
  • Using selective MMP-2/9 inhibitor SB-3CT (2-[[(4-phenoxyphenyl)sulfonyl]methyl]-thiirane) or their neutralizing antibodies or Cav-1 siRNA, we found that MMP-2 was the major enzyme mediating OGD-induced occludin degradation, while Cav-1 was responsible for claudin-5 redistribution. (qxmd.com)
  • Anti-OAP-1, anti-OSP/claudin-11, and anti-beta1 integrin antibodies inhibited migration of primary oligodendrocytes, and migration was impaired in OSP/claudin-11-deficient primary oligodendrocytes. (sigmaaldrich.com)
  • Inhibition of hepatitis C virus infection by anti-claudin-1 antibodies is mediated by neutralization of E2-CD81-claudin-1 associations. (bham.ac.uk)
  • Polyclonal antibodies specific for claudin-7 and -12 were not available. (asnjournals.org)
  • C) Cell lysates from Huh7.5.1 cells were pulled down by GST-CD81-N-tail, GST-CD81-C-tail, GST-occludin-tail, GST-SRB1-tail, GST-claudin-1 tail or GST with two extra washes. (asm.org)
  • Metformin promoted ZO-1, occludin and claudin-5 rearrangement and lessened IgG and Evans blue extravasation. (nih.gov)
  • A) Occludin, ZO-1 and claudin-5 expression in sham group, NS and metformin-treated mice at 3 days following tMCAO. (nih.gov)
  • B) Representative result of occludin, ZO-1 and claudin-5 expression at 1 and 3 days after tMCAO. (nih.gov)
  • Bar graphs show a quantification of occludin, ZO-1 and claudin-5 expression. (nih.gov)
  • Result indicated that occludin and ZO-1 were continuously located on the margin of ECs in sham group, claudin-5 was continuously located along ECs, and fewer gaps were formed in the metformin-treated group (Figure 4A). (nih.gov)
  • Effects of claudin-2, occludin or ZO-1 siRNA transfection on transepithelial electrical resistance (TER) in HK-2 cell monolayers. (figshare.com)
  • TER was significantly decreased by claudin-2 siRNA transfection, but significantly increased by siRNA transfection against occludin or ZO-1. (figshare.com)
  • The objectives of this study were to investigate the localization of tight junctions and the modulation of zonula occludens- (ZO-) 1, occludin and claudin-1 expression by estrogen in castrated female rat vagina. (nih.gov)
  • The cellular localization and expression of ZO-1, occludin, and claudin-1 were determined in each group by immunohistochemistry and western blot. (nih.gov)
  • Expression of ZO-1, occludin, and claudin-1 was significantly decreased after ovariectomy and was restored to the level of the control after estrogen replacement. (nih.gov)
  • In contrast to occludin, which induces only a small number of short strands at cell-cell contact sites when introduced into fibroblasts lacking tight junctions, claudin-1 and -2 induce networks of strands characteristic of true tight junctions. (thermofisher.com)
  • Though inconclusive, these findings suggest that claudin-1 and -2 are major structural components of TJ strands and that occludin plays some other accessory role. (thermofisher.com)
  • In order to investigate the regulation of expression of tight-junction molecules and of function during bone differentiation, osteoblast-like MC3T3-E1 cells and osteocyte-like MLO-Y4 cells were treated with IGF-I. In both MC3T3-E1 cells and MLO-Y4 cells, the tight-junction molecules occludin, claudin-1, -2, and -6, and the gap-junction molecule connexin 43 (Cx43) were detected by reverse transcription with polymerase chain reaction. (elsevier.com)
  • This same OGD treatment also led to rapid degradation of tight junction protein occludin and dissociation of claudin-5 from the cytoskeleton, which contributed to OGD-induced endothelial barrier disruption. (qxmd.com)
  • Moreover, occludin protein loss and claudin-5 redistribution were detected in ischemic cerebromicrovessels. (qxmd.com)
  • These data indicate that cerebral ischemia initiates two rapid parallel processes, MMP-2-mediated occludin degradation and Cav-1-mediated claudin-5 redistribution, to cause BBB disruption at early stroke stages relevant to acute thrombolysis. (qxmd.com)
  • In rat brain glioma model and BTB model in vitro, we find that the protein expression levels of ZO-1, occludin, and claudin-5 are attenuated by BK induction. (qxmd.com)
  • The redistribution of ZO-1, occludin, and claudin-5 and rearrangement of F-actin in brain microvascular endothelial cells are observed at the same time. (qxmd.com)
  • FAK is an integrated component of the blood-testis barrier (BTB) involved in regulating Sertoli cell adhesion via its effects on the occludin-zonula occludens-1 complex. (pnas.org)
  • Recent studies have shown that FAK is a regulatory protein that modulates TJ integrity at the BTB via its effects on the phosphorylation status of the adhesion protein complexes, such as the occludin-zonula occludens (ZO)-1 complex ( 6 ). (pnas.org)
  • On the contrary, overexpression of TUSC3 significantly enhanced EMT progress by upregulating claudin-1 expression. (nih.gov)
  • Overexpression of Slug and Snail in MDCK (Madin-Darby canine kidney) cells down-regulated Claudin-1 at protein and mRNA levels. (biochemj.org)
  • Taken together, our results indicate that overexpression of claudin-2 promotes self-renewal within CRC stem-like cells, suggesting a potential role for this protein as a therapeutic target in CRC. (aacrjournals.org)
  • Overexpression of OSP/claudin-11 or OAP-1 induced proliferation in an oligodendrocyte cell line. (sigmaaldrich.com)
  • Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate. (semanticscholar.org)
  • Overexpression studies suggest that tight junction leakiness in these two strains of MDCK cells is conferred by expression of the tight junction protein claudin-2. (uab.edu)
  • In addition, Snail and Slug are able to effectively repress human Claudin - 1 -driven reporter gene constructs containing the wild-type promoter sequence, but not those with mutations in two proximal E-box elements. (biochemj.org)
  • Taken together, these results support the hypothesis that Claudin - 1 is a direct downstream target gene of Snail family factors in epithelial cells. (biochemj.org)
  • However, the effects of microRNA-1303 (miR-1303) on gastric cancer (GC) cells and the upstream regulation of GC-associated claudin-18 gene (CLDN18) remain unclear. (springer.com)
  • The claudin gene family: expression in normal and neoplastic tissues. (springer.com)
  • To study the basolateral function of claudin-7, claudin-7 gene silencing experiments were carried out in a lung cancer cell line using the lentivirus shRNA approach. (aacrjournals.org)
  • We also generated claudin-7-deficient (Cldn7 −/− ) mice using targeted-gene deletion method. (aacrjournals.org)
  • This gene encodes a member of the claudin family. (thermofisher.com)
  • The gene expression characteristics of claudin-low-like MPA/DMBA-induced tumors accurately reflected those of human claudin-low tumors, including epithelial-mesenchymal transition phenotype, high level of immune activation, and low degree of differentiation. (uio.no)
  • In our previous study, we found that claudin-6 is preferentially expressed in mammary epithelial cells and functions as a potential breast cancer suppressor gene [ 20 ], which is supported by the follow-up study of Osanai [ 21 ]. (biomedcentral.com)
  • The protein encoded by this intronless gene belongs to the claudin family. (genetex.com)
  • Here, we show that this is not because of relocalization of claudin-2 into the cytosolic pool but primarily due to a decrease in gene expression. (deepdyve.com)
  • Claudin-6 is a candidate tumor suppressor gene in breast cancer, and has been shown to be regulated by DNA methylation and histone modification in breast cancer lines. (biomedcentral.com)
  • Immunohistochemical staining was used to study protein expression in both tumor and the adjacent nonneoplastic mucosa of claudin 1, 4, and 7. (diva-portal.org)
  • Knockdown of GLI1 expression in claudin-low cell lines resulted in reduced cell viability, motility, clonogenicity, self-renewal, and reduced tumor growth of orthotopic xenografts. (biomedcentral.com)
  • Interestingly, no significant association was found between claudin 1 and nodal involvement, tumor grade or tumor size. (biomedcentral.com)
  • miR-1303 was significantly overexpressed whereas claudin-18 was downregulated in GC tissues and cell lines, which was significantly associated with tumor size, location invasion, histologic type and tumor-node-metastasis stage. (springer.com)
  • In mice, the medroxyprogesterone acetate (MPA) and 7,12-dimethylbenzanthracene (DMBA)-induced mammary tumor model yields a heterogeneous set of tumors, a subset of which display claudin-low features. (uio.no)
  • Endoglin tumor cell expression was associated with E-cadherin and β-catenin expression (p=0.001 and p=0.068), but not with claudin-1 expression (p=0.299). (ejmjih.com)
  • A particular characteristic of EMT is the downregulation of E-cadherin expression, which causes disruption of cell-cell junctions and dissemination of cells from the primary tumor ( 1 ). (aacrjournals.org)
  • article{6717062, abstract = {Hepatitis C virus (HCV) infection is a leading cause of liver cirrhosis and cancer(1). (ugent.be)
  • Claudin-1 is a tight junction transmembrane protein. (frontiersin.org)
  • Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. (ebi.ac.uk)
  • Claudin-5 (CLDN-5), a tetra-transmembrane protein is a key component of the TJ seal that prevents the paracellular diffusion of drugs into the CNS. (aspetjournals.org)
  • Claudin-10 exists in at least two functional isoforms, claudin-10a and claudin-10b, that differ in their first transmembrane segment and first extracellular segment. (nih.gov)
  • Claudin molecules bear four transmembrane domains with both N- and C-termini located in the cytoplasm. (biologists.org)
  • Knockout of claudin-1, a transmembrane adhesive protein in TJs, in mice caused leakage of the TJ barrier in sebaceous glands and incomplete degradation of the plasma membrane and nuclei during holocrine secretion. (elsevier.com)
  • B ) HIF-1α expression was assessed by Western blot of nuclear protein lysate from esophageal epithelial cells exposed to a time course (0, 4, 24, 48, 72 hours) of experimental hypoxia compared with duration-matched normoxic cells and quantified by densitometry. (jci.org)
  • Additionally, increased claudin-11 protein was observed by Western blot. (nih.gov)
  • Downregulation of claudin 1, a critical tight junction protein, has been correlated with increased invasiveness in breast cancer. (sigmaaldrich.com)
  • Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). (cellsignal.com)
  • Claudin-7 is a unique TJ membrane protein in that it has a stronger basolateral membrane distribution than that of apical TJs in epithelial cells. (aacrjournals.org)
  • These data show for the first time that the ERK 1/2 signaling pathway negatively controls claudin-2 expression in mammalian renal epithelial cells and provide evidence for regulation of tight junction paracellular transport by alterations in claudin composition within tight junction complexes. (uab.edu)
  • For example, VE-cadherin, claudin-5, or PECAM-1 have only been found in endothelial but not in epithelial cells. (ahajournals.org)
  • Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. (mdpi.com)
  • High GLI1 expression is a property of claudin-low cells and tumors and correlates with markers of EMT and breast cancer stem cells. (biomedcentral.com)
  • Claudin-low tumors are mainly triple-negative invasive ductal carcinomas with a high frequency of metaplastic and medullary differentiation. (biomedcentral.com)
  • In these tumors, the claudin 1 protein, usually localized in the cell membrane, is often mislocalized to the cytoplasm. (biomedcentral.com)
  • The association of high claudin 1 protein levels observed in tumors derived from older women with BLBC, suggests that claudin 1 has the potential to serve as a marker which can identify a specific subgroup of patients within the BLBC subtype and thus, further contribute to the characterization of these ill-defined breast cancers. (biomedcentral.com)
  • Here we report that elevated claudin-2 expression in stage II/III colorectal tumors is associated with poor recurrence-free survival following 5-FU-based chemotherapy, an outcome in which CSC play an instrumental role. (aacrjournals.org)
  • Neither the genomic characteristics of MPA/DMBA-induced claudin-low tumors nor those of human claudin-low breast tumors have been thoroughly explored. (uio.no)
  • A publicly available dataset was queried to explore the genomic characteristics of human claudin-low breast cancer and to validate findings in the murine tumors. (uio.no)
  • Half of MPA/DMBA-induced tumors showed a claudin-low-like subtype. (uio.no)
  • however, none accurately delineated claudin-low-like tumors. (uio.no)
  • There was an elevated expression of the immunosuppressive genes PTGS2 (encoding COX-2) and CD274 (encoding PD-L1) in human and murine claudin-low tumors. (uio.no)
  • Furthermore, using genetic manipulations of claudin-1 expression in colon cancer cell lines, we demonstrate a role for claudin-1 in the regulation of epithelial to mesenchymal transition (EMT), growth of xenografted tumors and metastasis in athymic mice. (elsevier.com)
  • Results: Forty-six (66.7%), 67 (97.1%), and 67 (97.1%) of the tumors, expressed immunostaining for claudin-1, E-cadherin and β-catenin, respectively. (ejmjih.com)
  • The present invention relates to novel peptides derived from Claudin-6 (CLDN6), complexes comprising such peptides bound to recombinant MHC molecules, and cells presenting said peptide in complex with MHC molecules. (wipo.int)
  • Our simulations indicate that claudin-15 forms well-defined channels for ions and molecules and otherwise "seals" the paracellular space through hydrophobic interactions. (rupress.org)
  • The simulations suggest that the selectivity filter is formed by a cage of four aspartic acid residues (D55), contributed by four claudin-15 molecules, which creates a negative electrostatic potential to favor cation flux over anion flux. (rupress.org)
  • Although HCV entry requires the CD81 co-receptor, and other host molecules have been implicated, at least one factor critical to this process remains unknown (reviewed in refs 1-3). (ox.ac.uk)
  • It was recently demonstrated that heterogeneous claudin species are copolymerized to form individual TJ strands as heteropolymers and that, between adjacent TJ strands, claudin molecules adhere to each other in both homotypic and heterotypic manners, except in some combinations ( 14 ). (asnjournals.org)
  • miR-1303 could bind to the putative binding sites in CLDN18 mRNA 3′-UTR and visibly lower the expression of claudin-18. (springer.com)
  • Knockdown of claudin-7 by lentivirus claudin-7 shRNA downregulated integrin β1 at both mRNA and protein levels. (aacrjournals.org)
  • Suppression of claudin-7 also upregulated matrix metalloproteinase-3 (MMP-3) at both mRNA and protein levels and downregulated several collagens, such as type V α1, type XV α1, and type XVI α1, at the mRNA level. (aacrjournals.org)
  • Real-time RT-PCR of laser microdissected tubules showed an up-regulation of claudin-11 mRNA in TIN. (nih.gov)
  • Western blotting and RT-PCR consistently revealed that the level of ASK1 protein and mRNA was upregulated in MCF-7 cell clones transfected with claudin-6. (biomedcentral.com)
  • TUSC3 accelerates cancer growth and induces epithelial-mesenchymal transition by upregulating claudin-1 in non-small-cell lung cancer cells. (nih.gov)
  • In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. (mdpi.com)
  • Cell lysates from Huh7.5.1 cells were pulled down by GST-claudin-1 tail or GST followed by Western blotting. (asm.org)
  • D and E) Immunoblots showing the association of Sec24C with claudin-1 in transfected HEK293T cells by a coimmunoprecipitation assay. (asm.org)
  • HEK293T cells were transfected with Flag-claudin-1, Flag-SRB1, or PCMV-Tag2 empty vector for 48 h. (asm.org)
  • F) Coimmunoprecipitation of endogenous claudin-1 with Sec24C in Huh7.5.1 cells. (asm.org)
  • G) Colocalization of claudin-1 with Sec24C in Huh7.5.1 cells. (asm.org)
  • Huh7.5.1 cells were fixed by PFA and immunofluorescently labeled for claudin-1 (green) and Sec24C (red). (asm.org)
  • C) Cell surface claudin-1 was quantified by FACS assay in nonpermeabilized cells. (asm.org)
  • D) Intercellular claudin-1 was quantified by FACS assay in permeabilized cells. (asm.org)
  • Claudin 1 promotes migration and increases sensitivity to tamoxifen and anticancer drugs in luminal-like human breast cancer cells MCF7. (sigmaaldrich.com)
  • Furthermore, metformin activated AMPK signaling pathway and alleviated oxygen-glucose deprivation-induced ICAM-1 expression in bEND.3 cells (P (nih.gov)
  • For claudin 4, the percentage of cells staining positively was also significantly reduced (p = 0.04). (diva-portal.org)
  • The recently identified claudin-low subtype of breast cancer is enriched for cells with stem-like and mesenchymal-like characteristics. (biomedcentral.com)
  • Claudin 1 knockdown in BT-20 cells resulted in decreased cell migration. (biomedcentral.com)
  • We observed non-canonical activation of GLI1 in claudin-low and EMT cell lines, and identified crosstalk with the NF¿B pathway.ConclusionsThis work highlights the importance of GLI1 in the maintenance of characteristics of metastatic breast cancer stem cells. (diagenode.com)
  • In patient-derived organoids, primary cells, and cell lines, claudin-2 promoted CRC self-renewal in vitro and in multiple mouse xenograft models. (aacrjournals.org)
  • Next-generation sequencing in ALDHHigh cells revealed that claudin-2 regulated expression of nine microRNAs known to control stem cell signaling. (aacrjournals.org)
  • We found that claudin-7 knockdown (KD) cells showed disrupted cell-matrix interactions. (aacrjournals.org)
  • Consequently, when claudin-7 KD cells were plated on the uncoated glass surface, they were unable to attach to the glass and died the day after plating. (aacrjournals.org)
  • This cell-matrix defect can be rescued by transfecting claudin-7 or integrin β1 back to the claudin-7 KD cells. (aacrjournals.org)
  • Here we found more T cells expressing the transcription factor PU.1 and interleukin 9 (IL-9) in patients with ulcerative colitis. (nature.com)
  • Because TJs of basal cells are primarily composed of claudin-11, claudin-11-deficient (Cld11 -/- ) mice were generated with an expectation that the compartmentalization in stria vascularis in these mice would be affected. (biologists.org)
  • The expression of claudin-1 in MC3T3-E1 cells after IGF-I treatment was mainly upregulated via a mitogen-activated protein (MAP)-kinase pathway and, in part, modulated by a PI3-kinase pathway, whereas Cx43 expression and the mediated gap-junctional intercellular communication protein did not contribute to the upregulation. (elsevier.com)
  • Furthermore, in MC3T3-E1 cells during wound healing, upregulation of claudin-1 was observed together with an increase of IGF-I and type I collagen. (elsevier.com)
  • Previous studies have demonstrated that claudin-6 functions as a cancer suppressor in human MCF-7 breast cancer cells. (biomedcentral.com)
  • In MDCK II cells, barrier improvement by claudin-8 is accompanied by a reduction of endogenous claudin-2 protein at the tight junction. (deepdyve.com)
  • To test whether replacement of cation-permeable claudin-2 mediates the effect of claudin-8 on the electrophysiological phenotype of the host cell line, we expressed claudin-8 in high-resistance MDCK I cells, which lack endogenous claudin-2. (deepdyve.com)
  • In the seminiferous epithelium, the blood-testis barrier (BTB) created by coexisting tight junction (TJ) and basal ectoplasmic specialization [basal ES, a testis-specific adherens junction (AJ)] between Sertoli cells near the basement membrane is one of the tightest blood-tissue barriers in the mammalian body ( 1 ). (pnas.org)
  • A major difference between HT29 and MDCK I cells, which have low and high basal transepithelial electrical resistance, respectively, was the absence of claudin 2 in the latter. (deepdyve.com)
  • Platelet endothelial cell adhesion molecule-1, stage-specific embryonic antigen-1, and Flk-1 mark distinct populations of mouse embryonic stem cells during differentiation toward hematopoietic/endothelial cells. (semanticscholar.org)
  • Inhibition of Src and p38 MAP kinases suppresses the change of claudin expression induced on dedifferentiation of primary cultured parotid acinar cells. (semanticscholar.org)
  • Extracellular signal-regulated kinase (ERK) 1/2 activation by hepatocyte growth factor treatment of MDCK strain II cells inhibited claudin-2 expression and transiently increased TER. (uab.edu)
  • Transfection of constitutively active mitogen-activated protein kinase/extracellular signal-regulated kinase kinase into MDCK strain II cells also inhibited claudin-2 expression and increased TER. (uab.edu)
  • MDCK strain I cells have higher levels of active ERK 1/2 than do MDCK strain II cells. (uab.edu)
  • U0126 treatment of MDCK strain I cells decreased active ERK 1/2 levels, induced expression of claudin-2 protein, and decreased TER by ∼20-fold. (uab.edu)
  • AJOU Open Repository: Claudin-1 induces epithelial-mesenchymal transition through activation of the c-Abl-ERK signaling pathway in human liver cells. (ajou.ac.kr)
  • 1-5 In general, signal transduction by junctional components is sustained and directed to establish homeostasis of the cells. (ahajournals.org)
  • Claudin-5 and -15 were detected only in endothelial cells. (asnjournals.org)
  • Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). (cellsignal.com)
  • Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. (jci.org)
  • L2-IL5OXA mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal epithelial-targeted stabilized HIF-1α. (jci.org)
  • In addition, TUSC3 knockdown suppressed epithelial-mesenchymal transition by downregulating the expression of claudin-1, which plays an indispensable role in EMT progress. (nih.gov)
  • We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. (mdpi.com)
  • Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant mice. (mdpi.com)
  • In conclusion, SCF/c-kit-JNK/AP-1 signaling pathway significantly promoted claudin-3 expression in colonic epithelium and CRC, which could contribute to epithelial barrier function maintenance and to CRC development. (mdpi.com)
  • In addition, metformin activated AMPK phosphorylation, inhibited NF-κB activation, down-regulated cytokine (IL-1β, IL-6, TNF-α) and ICAM-1 expression following tMCAO (P (nih.gov)
  • We aimed our study to investigate whether claudin protein expression and methylation of CLDN can influence the tumorigenesis of colorectal cancer. (diva-portal.org)
  • Significantly, in invasive human breast tumours, high levels of Snail and Slug correlated with low levels of Claudin - 1 expression. (biochemj.org)
  • Expression of ZO-1 was diffuse in all groups, with the highest intensity in the superficial epithelium in the control group. (nih.gov)
  • Using a high throughput inhibitor screen, we identified high expression of glioma-associated oncogene homolog 1 ( GLI1) , the effector molecule of the hedgehog (Hh) pathway, as a critical determinant of cell lines that have undergone an epithelial to mesenchymal transition (EMT). (biomedcentral.com)
  • Immunohistochemical analysis of this patient cohort revealed a significant association between high claudin 1 expression and BLBCs in women 55 years of age and older. (biomedcentral.com)
  • More importantly, our studies strongly suggest that claudin 1 directly participates in promoting breast cancer progression, possibly through the alteration of expression of EMT genes. (biomedcentral.com)
  • The expression of miR-1303 and claudin-18 in GC tissues and gastric cancer cell lines were detected by qRT-PCR and western blotting, respectively. (springer.com)
  • Several authors have reported conflicting results of claudin-1 expression in colorectal carcinoma. (oatext.com)
  • The goal of this study is to examine claudin-1 expression in a tissue microarray of colorectal cancer and metastases, simultaneously, in order to assess the prognostic value of this protein in colorectal cancer. (oatext.com)
  • This study analysed the expression of claudin-1 by immunohistochemistry in 99 tissue samples (including 33 colorectal cancer cases with 33 concordant lymph node metastases and 33 concordant cancer-adjacent normal tissues, all from the same patients). (oatext.com)
  • This is the first study to demonstrate the expression of claudin-1 protein across a sample of colorectal cancer and its metastases, simultaneously. (oatext.com)
  • We report loss of claudin-1 expression in the lymph node metastases in the majority of cases. (oatext.com)
  • Claudin-1 expression is frequently altered in several cancers, including upregulation and down regulation [13]. (oatext.com)
  • Deletion of claudin-7 reduced integrin α2 expression and altered its localization. (aacrjournals.org)
  • Here, we determined the expression and distribution of claudin-11 at the human BTB in seminiferous tubules with normal spermatogenesis (NSP) and TIN. (nih.gov)
  • Therefore, the disruption of the BTB is related to a dysfunction of claudin-11 and not to a failure of its expression. (nih.gov)
  • Transepithelial resistance and claudin expression in trout RTgill-W1 cell line: Effects of osmoregulatory hormones. (genscript.com)
  • Abnormal expression and mislocalization of Claudin 7 are frequently observed in epithelial-derived lung, breast, head, and neck cancers. (perkinelmer.com)
  • Immunohistochemical analysis was performed to evaluate the ASK1 protein expression and the correlation between ASK1, claudin-6 and clinicopathological features in 85 samples of breast invasive ductal carcinomas (IDC). (biomedcentral.com)
  • Western blotting and RT-PCR was carried out to examine the expression of ASK1 and claudin-6 in MCF-7 cell clones transfected with claudin-6. (biomedcentral.com)
  • Here, we report a highly increased expression for claudin-1 in human primary colon carcinoma and metastatic tissues and cell lines derived from similar sources with relatively frequent nuclear localization. (elsevier.com)
  • Disruption of tight junctions, including changes in claudin-5 expression, is common in HIV-1-infected patients. (elsevier.com)
  • Andras, IE & Toborek, MJ 2011, HIV-1-induced alterations of claudin-5 expression at the blood-brain barrier level . (elsevier.com)
  • U0126 treatment also induced claudin-2 expression and decreased TER in a high resistance mouse cortical collecting duct cell line (94D). (uab.edu)
  • A significant association was seen between claudin-1 and Ecadherin expression (p=0.002), as well with β-catenin (p=0.009). (ejmjih.com)
  • However, the expression of claudin-6 in breast invasive ductal carcinomas and correlation with clinical behavior or expression of other markers is unclear. (biomedcentral.com)
  • We have investigated the expression of claudin-6, MeCP2, HDAC1, H3Ac and H4Ac in 100 breast invasive ductal carcinoma tissues and 22 mammary gland fibroadenoma tissues using immunohistochemistry. (biomedcentral.com)
  • Claudin-6 expression was inversely correlated with lymph node metastasis ( P = 0.021). (biomedcentral.com)
  • Expression of occluding-1 was increased. (hindawi.com)
  • Role of claudin species-specific dynamics in reconstitution and remodeling of the zonula occludens. (ebi.ac.uk)
  • Manner of interaction of heterogeneous claudin species within and between tight junction strands. (ebi.ac.uk)
  • Most cell types express more than two claudin species in various combinations to constitute TJ strands. (biologists.org)
  • Importantly, detailed analyses suggested that variations in the tightness of individual paired TJ strands are determined by the combinations and mixing ratios of claudin species ( 3 , 15 - 17 ). (asnjournals.org)
  • Here, we address how the interaction of heterologously expressed claudin-8 with endogenous claudin isoforms impacts epithelial barrier properties. (deepdyve.com)
  • In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. (mdpi.com)
  • a) Immunohistochemistry of claudin-1 in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy plus 17β-estradiol treatment (Ovx + Est) groups. (nih.gov)
  • While several of these biological processes are features of the CSC phenotype, a role for claudin-2 in the regulation of these has not been identified. (aacrjournals.org)
  • Among these, miR-222-3p was instrumental for the regulation of self-renewal by claudin-2, and enhancement of this self-renewal required activation of YAP, most likely upstream from miR-222-3p. (aacrjournals.org)
  • The mechanisms of claudin regulation and their exact roles in normal physiology and disease are being elucidated, but much work remains to be done. (hindawi.com)
  • We also observed an inverse relationship between upregulation of claudin 1 and TGFβ. (sigmaaldrich.com)
  • Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. (mdpi.com)
  • Claudin-1 is an integral membrane protein component of tight junctions. (biochemj.org)
  • The claudin-1 assembly with tight junctions is regulated by post translational modifications. (biomedcentral.com)
  • and Thr 191 may provide an on/off switch to regulate assembly of claudin-1 at tight junctions. (biomedcentral.com)
  • In addition to that, tight junctions also play critical roles in maintaining cell polarity and signal transductions [1,2]. (oatext.com)
  • Double immunogold labeling demonstrated a co-localization of claudin-11 and ZO-1 at the inter-Sertoli cell junctions. (nih.gov)
  • Thus, Claudin-4 forms channels through the tight junctions that discriminate against Na+ ions and are indifferent to Cl- ions. (thermofisher.com)
  • Oligodendrocyte-specific protein (OSP)/claudin-11 is a major component of central nervous system myelin and forms tight junctions (TJs) within myelin sheaths. (sigmaaldrich.com)
  • Claudin-8 also affects the trafficking of claudin-2, which was displaced specifically from the junctions at which claudin-8 was inserted. (deepdyve.com)
  • The integrity of the brain endothelium is regulated by intercellular tight junctions, which also play a critical role in HIV-1-entry into the brain. (elsevier.com)
  • Tight junctions (TJ) have been demonstrated to be responsible for this intercellular sealing ( 1 - 3 ). (asnjournals.org)
  • Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK (human embryonic kidney)-293. (nih.gov)
  • Recombinant human Claudin 1 protein, around 150-250aa. (lucerna-chem.ch)
  • We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin - 1 promoter. (biochemj.org)
  • We also interrogated the outcome of claudin 1 knockdown in a human BLBC cell line, BT-20. (biomedcentral.com)
  • The tight junction (TJ) protein claudin-2 is overexpressed in human CRC where it enhances cell proliferation, colony formation, and chemoresistance in vitro. (aacrjournals.org)
  • We conclude that claudin-11 constitutes a TJ protein at the human BTB. (nih.gov)
  • The AlphaLISA ® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps). (perkinelmer.com)
  • Human claudin-low breast cancers carried a distinct set of genomic characteristics, in particular a relatively low burden of mutations and copy number aberrations. (uio.no)
  • Reactivity has been confirmed with rat, human and mouse Claudin-5 using rat lung, mouse kidney, mouse small intestine, mouse lung homogenates, human colon tissue, and CACO-2 human cell line. (thermofisher.com)
  • Materials and Methods: Surgical specimens from 69 patients with colorectal cancer were immunostained for claudin-1, E-cadherin, β-catenin, endoglin and CD31. (ejmjih.com)
  • Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit signaling significantly promoted activator protein-1 (AP-1) binding with CLDN-3 promoter and enhanced its transcription activity. (mdpi.com)
  • Regression analysis however, showed a significant positive association between claudin 1 and claudin 4, even though claudin 4 did not significantly correlate with patient age. (biomedcentral.com)
  • The intestinal bacterial richness (Chao 1 and OTUs) was significantly lower in OAP than in NAP rats. (nature.com)
  • Abdominal pain and/or discomfort are common symptoms in various disease situations [ 1 - 3 ] and the severity of abdominal symptoms significantly influences patients' quality of life and psychological disturbances [ 4 , 5 ]. (hindawi.com)
  • Collectively, these studies reveal HIF-1α's critical role in maintaining barrier and highlight the HIF-1α/claudin-1 axis as a potential therapeutic target for EoE. (jci.org)
  • In mouse testis, claudin-11 is responsible for the formation of specific parallel TJ strands of the blood-testis barrier (BTB). (nih.gov)
  • HIV-1 crosses the blood-brain barrier (BBB) early in the course of systemic infection and resides in brain macrophages and microglia. (elsevier.com)
  • Claudin-1 knockout resulted in the accumulation of incompletely degenerated sebocytes in sebaceous ducts, suggesting that the TJ barrier was necessary for differentiation of holocrine secretion. (elsevier.com)
  • Using immunofluorescent microscopy and biochemistry methods, we found that claudin-7 co-localized and co-immunoprecipitated with integrin α2 in Cldn7 +/+ mouse intestines where claudin-7 was highly expressed. (aacrjournals.org)
  • However, recent studies suggest that claudin 1 contributes to the progression of some molecular subtypes of breast cancer. (sigmaaldrich.com)
  • Collectively, our results suggest that claudin 1 has the potential to be used as a predictive marker for treatment efficacy for specific breast cancer patient subgroups. (sigmaaldrich.com)
  • The data provide novel insight into the molecular TJ architecture consistent with a model with an antiparallel double-row cis-arrangement of classic claudin protomers within strands. (nih.gov)
  • To understand the molecular basis of claudin-1/CD81 association we previously produced and purified milligram quantities of functional, full-length CD81, which binds a soluble form of HCV E2 glycoprotein (sE2). (chalmers.se)
  • Claudin-low breast cancer is a molecular subtype associated with poor prognosis and without targeted treatment options. (uio.no)
  • Here, we use molecular dynamics simulations to build and refine an atomic model of claudin-15 channels and study its transport properties. (rupress.org)
  • Their structural and functional similarities and differences make them ideal subjects to study determinants of claudin charge selectivity and pore formation. (nih.gov)
  • Thus, our model provides an atomic view of claudin channels, their transport function, and a potential three-dimensional organization of its selectivity filter. (rupress.org)
  • Immunostaining of claudin-11 revealed intense signals at the basal BTB region in seminiferous epithelium with NSP. (nih.gov)
  • This is attributable to the tightly packed actin filament bundles sandwiched between cisternae of endoplasmic reticulum and the apposing Sertoli cell plasma membrane at the basal ES, the hallmark ultrastructure of the BTB ( 1 ). (pnas.org)
  • However, the BTB undergoes cyclical restructuring to allow the transit of preleptotene spermatocytes from the basal to the adluminal compartment in the rat ( 1 , 2 ), implying that these filament bundles undergo extensive reorganization. (pnas.org)
  • Using FRET analysis, the proximity of claudin N- and C-termini integrated in homopolymeric strands composed of claudin-3 or of heteropolymeric strands composed of claudin-1 and claudin-3 were determined. (nih.gov)
  • Claudin-6 shares ~25-70% overall similarity with other claudin family members at the amino acid level, displaying highest similarity to claudin-9. (ebi.ac.uk)
  • One family member, claudin-1, also associates with tetraspanin CD81 as part of a receptor complex that is essential for hepatitis C virus (HCV) infection of the liver. (chalmers.se)
  • Belongs to the claudin family. (abcam.com)
  • OSP/claudin-11 forms a complex with a novel member of the tetraspanin super family and beta1 integrin and regulates proliferation and migration of oligodendrocytes. (sigmaaldrich.com)
  • Claudin-6 was identified through searching expressed sequence tag (EST) databases for sequences similar to claudin-1 and -2 [ PMID: 9892664 ]. (ebi.ac.uk)
  • Your search returned 4 Claudin 7 ELISA ELISA Kit across 1 supplier. (biocompare.com)
  • In mammals, humans have 23 claudin genes, whereas rats and mice have 24 genes ( Lal-Nag and Morin, 2009 ). (frontiersin.org)
  • Ninety-eight adult male CD-1 mice underwent 90-minute transient middle cerebral artery occlusion (tMCAO). (nih.gov)
  • Here we generated claudin-1-deficient mice and found that the animals died within 1 d of birth with wrinkled skin. (rupress.org)
  • J:167016 Kobayashi K, Yasui M, Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice. (jax.org)
  • Measuring the Claudin 7 levels is important for the detection of different types of cancers and is also an important target for therapeutic intervention. (perkinelmer.com)
  • Objectives: Intercellular adhesion mediated by claudin and cadherin/catenin complex is a prerequisite of epithelial integrity and differentiation and has been suggested to be frequently disturbed in cancers. (ejmjih.com)
  • Both isoforms act as selective paracellular ion channels, either for anions (claudin-10a) or for cations (claudin-10b). (nih.gov)
  • We conclude that recombinant, correctly-folded, full-length claudin-1 can be produced in yeast membranes, that it can be extracted in different oligomeric forms that do not bind sE2 and that a dynamic preparation can form a specific complex with CD81 in vitro in the absence of any other cellular components. (chalmers.se)
  • Claudin-2 is typical of leaky, water-transporting epithelia, such as the kidney proximal tubule, whereas claudin-10b is present in numerous epithelia, including water-impermeable segments of the loop of Henle. (biologists.org)
  • Our results uncover crosstalk between NFκB and GLI1 signals and suggest that targeting these pathways may be effective against the claudin-low breast cancer subtype. (biomedcentral.com)
  • Our findings show that the claudin-low breast cancer subtype is not demarcated by specific genomic aberrations, but carries potentially targetable characteristics warranting further research. (uio.no)
  • The structure shows that C-CPE forms extensive hydrophobic and hydrophilic interactions with the two extracellular segments of claudin-19. (rcsb.org)
  • 4. The skin care composition of claim 1, wherein said pomegranate seed oil is present in an amount from about 0.001 to about 20% by weight of total composition ingredients. (freepatentsonline.com)
  • 1 Growing evidence indicates that endothelial cell-to-cell adhesion is accompanied by intracellular signaling. (ahajournals.org)
  • TGF-β) receptors, FAK plays a central role in transducing signals to the actin cytoskeleton to elicit F-actin remodeling (e.g., formation of lamellipodia and stress fibers) during cell adhesion and migration ( 1 , 7 ). (pnas.org)
  • In this study, the localization of claudin-1 in maturation ameloblasts was examined using immunofluorescence microscopy. (frontiersin.org)
  • Localization of claudin-1 in the Golgi apparatus may reflect rapid tight junction turnover during enamel maturation. (frontiersin.org)