1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine: A specific protein kinase C inhibitor, which inhibits superoxide release from human neutrophils (PMN) stimulated with phorbol myristate acetate or synthetic diacylglycerol.Isoquinolines: A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)PiperazinesProtein Kinase C: An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.Tetradecanoylphorbol Acetate: A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.DiglyceridesProtein Kinase Inhibitors: Agents that inhibit PROTEIN KINASES.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Receptors, sigma: A class of cell surface receptors recognized by its pharmacological profile. Sigma receptors were originally considered to be opioid receptors because they bind certain synthetic opioids. However they also interact with a variety of other psychoactive drugs, and their endogenous ligand is not known (although they can react to certain endogenous steroids). Sigma receptors are found in the immune, endocrine, and nervous systems, and in some peripheral tissues.Phorbol 12,13-Dibutyrate: A phorbol ester found in CROTON OIL which, in addition to being a potent skin tumor promoter, is also an effective activator of calcium-activated, phospholipid-dependent protein kinase (protein kinase C). Due to its activation of this enzyme, phorbol 12,13-dibutyrate profoundly affects many different biological systems.Staurosporine: An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)Sulfonamides: A group of compounds that contain the structure SO2NH2.Alkaloids: Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)Phorbol Esters: Tumor-promoting compounds obtained from CROTON OIL (Croton tiglium). Some of these are used in cell biological experiments as activators of protein kinase C.Sphingosine: An amino alcohol with a long unsaturated hydrocarbon chain. Sphingosine and its derivative sphinganine are the major bases of the sphingolipids in mammals. (Dorland, 28th ed)Calcimycin: An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Somatostatin: A 14-amino acid peptide named for its ability to inhibit pituitary GROWTH HORMONE release, also called somatotropin release-inhibiting factor. It is expressed in the central and peripheral nervous systems, the gut, and other organs. SRIF can also inhibit the release of THYROID-STIMULATING HORMONE; PROLACTIN; INSULIN; and GLUCAGON besides acting as a neurotransmitter and neuromodulator. In a number of species including humans, there is an additional form of somatostatin, SRIF-28 with a 14-amino acid extension at the N-terminal.Receptors, Somatostatin: Cell surface proteins that bind somatostatin and trigger intracellular changes which influence the behavior of cells. Somatostatin is a hypothalamic hormone, a pancreatic hormone, and a central and peripheral neurotransmitter. Activated somatostatin receptors on pituitary cells inhibit the release of growth hormone; those on endocrine and gastrointestinal cells regulate the absorption and utilization of nutrients; and those on neurons mediate somatostatin's role as a neurotransmitter.Pertussis Toxin: One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.Virulence Factors, Bordetella: A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.Calcium Channels: Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Sea Urchins: Somewhat flattened, globular echinoderms, having thin, brittle shells of calcareous plates. They are useful models for studying FERTILIZATION and EMBRYO DEVELOPMENT.Embryo, Nonmammalian: The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.Strongylocentrotus purpuratus: A species of SEA URCHINS in the family Strongylocentrotidae found on the Pacific coastline from Alaska to Mexico. This species serves as a major research model for molecular developmental biology and other fields.Lytechinus: A genus of SEA URCHINS in the family Toxopneustidae possessing trigeminate ambulacral plating.Hemicentrotus: A genus of SEA URCHINS in the family Strongylocentrotidae with a hemicyclic apical disk and short spines.Ovum: A mature haploid female germ cell extruded from the OVARY at OVULATION.Paracentrotus: A genus of SEA URCHINS in the family Echinidae found primarily on the western coasts of Ireland.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Myosins: A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Phosphoprotein Phosphatases: A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)Okadaic Acid: A specific inhibitor of phosphoserine/threonine protein phosphatase 1 and 2a. It is also a potent tumor promoter. (Thromb Res 1992;67(4):345-54 & Cancer Res 1993;53(2):239-41)Protein Phosphatase 2: A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.Hydrogen Sulfide: A flammable, poisonous gas with a characteristic odor of rotten eggs. It is used in the manufacture of chemicals, in metallurgy, and as an analytical reagent. (From Merck Index, 11th ed)Protein Phosphatase 1: A eukayrotic protein serine-threonine phosphatase subtype that dephosphorylates a wide variety of cellular proteins. The enzyme is comprised of a catalytic subunit and regulatory subunit. Several isoforms of the protein phosphatase catalytic subunit exist due to the presence of multiple genes and the alternative splicing of their mRNAs. A large number of proteins have been shown to act as regulatory subunits for this enzyme. Many of the regulatory subunits have additional cellular functions.Cystathionine gamma-Lyase: A multifunctional pyridoxal phosphate enzyme. In the final step in the biosynthesis of cysteine it catalyzes the cleavage of cystathionine to yield cysteine, ammonia, and 2-ketobutyrate. EC 18.104.22.168.Neuronal Plasticity: The capacity of the NERVOUS SYSTEM to change its reactivity as the result of successive activations.Aplysia: An opisthobranch mollusk of the order Anaspidea. It is used frequently in studies of nervous system development because of its large identifiable neurons. Aplysiatoxin and its derivatives are not biosynthesized by Aplysia, but acquired by ingestion of Lyngbya (seaweed) species.Action Potentials: Abrupt changes in the membrane potential that sweep along the CELL MEMBRANE of excitable cells in response to excitation stimuli.Invertebrate Hormones: Hormones produced by invertebrates, usually insects, mollusks, annelids, and helminths.Neurosecretion: The production and release of substances such as NEUROTRANSMITTERS or HORMONES from nerve cells.Thioridazine: A phenothiazine antipsychotic used in the management of PHYCOSES, including SCHIZOPHRENIA.Carcinoma, Non-Small-Cell Lung: A heterogeneous aggregate of at least three distinct histological types of lung cancer, including SQUAMOUS CELL CARCINOMA; ADENOCARCINOMA; and LARGE CELL CARCINOMA. They are dealt with collectively because of their shared treatment strategy.Lung Neoplasms: Tumors or cancer of the LUNG.Receptor, Epidermal Growth Factor: A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.QuinazolinesRNA Interference: A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.Cell Line, Tumor: A cell line derived from cultured tumor cells.
2,3,4,5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 --- 2,3,4,5-tetrahydro-7,8-dihydroxy-1- ... quinolizin-2-ol, 2-ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- MeSH D03.438.834.775 --- sparteine MeSH D03.438. ... 5-amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole MeSH D03.383.129.462.580.400 --- 4-chloro-7-nitrobenzofurazan MeSH D03.383. ... 5-amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole MeSH D03.383.312.649.290 --- fanft MeSH D03.383.312.649.308 --- furagin ...
... vitamin k 1 MeSH D02.455.849.291.523.500.844 --- vitamin k 2 MeSH D02.455.849.291.523.500.922 --- vitamin k 3 MeSH D02.455. ... 5-amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole MeSH D02.640.600.290 --- fanft MeSH D02.640.600.308 --- furagin MeSH ... 3-oxo-1,5-pentanediyl)bis(n,n-dimethyl-n-2-propenyl-), dibromide MeSH D02.092.146.325 --- p-dimethylaminoazobenzene MeSH ... vitamin k 1 MeSH D02.806.550.750 --- vitamin k 2 MeSH D02.806.550.875 --- vitamin k 3 MeSH D02.845.746.703 --- ...
2-methylpiperazine dihydrochloride H-NMR spectral analysis, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride C-NMR ...
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine: A specific protein kinase C inhibitor, which inhibits superoxide release from ... Isoquinolinesulfonyl)- 2- Methylpiperazine (H 7). Subscribe to New Research on 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine ... 1. Anoxia (Hypoxia) 05/01/2008 - "The performed study has shown that in rats submitted to hypoxia (3 h, 7% O2) at the 14th day ... 5. Neoplasms (Cancer) 01/01/1999 - "Four thin-section CT types of nodules were observed: (a) type L1 (4 of 19, 21 %), a fairly ...
108930-17-2 - OARGPFMFRLLKPF-UHFFFAOYSA-N - 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride - Similar structures ... RN: 108930-17-2. UNII: 55VT8U9HB1. InChIKey: OARGPFMFRLLKPF-UHFFFAOYSA-N. Note. *. A specific protein kinase C inhibitor, which ... 1S/C14H17N3O2S.2ClH/c1-11-9-16-7-8-17(11)20(18,19)14-4-2-3-12-10-15-6-5-13(12)14;;/h2-6,10-11,16H,7-9H2,1H3;2*1H. Download. ... S([[email protected]@]1[[email protected]@H](CNCC1). C). (c1c2c(cncc2). ccc1). (=O). =O.Cl.Cl. Download. ...
These results demonstrate that the calcium signaling pathway in PBMCs of CKD patients is defective already in CKD stage 2-3, ... Ingrid Lajdova 1 , Adrian Oksa, Dusan Chorvat Jr, Peter Topor, Viera Spustova ... 1 Department of Clinical and Experimental Pharmacology, Faculty of Medicine, Slovak Medical University, Bratislava, Slovak ... Methods: The study involved 20 healthy volunteers and 20 CKD stage 2-3 patients. The free cytosolic calcium concentration ([Ca( ...
Isoquinoline-5-sulfonic 2-methyl-1-piperazide; Linear Formula: C14H17N3O2S; find Sigma-Aldrich-I6891 MSDS, related peer- ... Isoquinolinesulfonyl). -. 2-. methylpiperazine, Isoquinoline-. 5-. sulfonic 2-. methyl-. 1-. piperazide * CAS Number 84477-87-2 ... 5 mg in glass bottle Biochem/physiol Actions Inhibitor of cyclic nucleotide dependent protein kinase (PKA) and protein kinase C ... Empirical Formula (Hill Notation) C14H17N3O2S ... 2-8°C Gene Information human ... CALCRL(10203). rat ... Calcrl( ...
5. Enhancement of the basal Ca2+ currents by FK960. A, time course of enhancement of peak currents by 0.1 μM FK960. Inset shows ... 1. Effect of FK960 on somatostatin-induced inhibition of Ca2+ currents in hippocampal neurons. Currents were evoked every 10 s ... 2, application of a GABAB receptor agonist baclofen (25 μM) reduced the peak currents with an average 16.33 ± 2.88% (n = 7) ... 2. Effect of FK960 on baclofen-induced inhibition of Ca2+ currents in hippocampal neurons. A, superimposed current traces were ...
Human promyelocytic leukemia HL-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25- ... dihydroxyvitamin D3 [1,25-(OH)2D3] or all-trans retinoic acid, respectively. In this study, the effect of capsaicin, an active ... Treatment of HL-60 cells with 5-30 microg/ml capsaicin for 72 h inhibited cell proliferation and induced a small increase in ... Capsaicin enhanced protein kinase C activity in 1,25-(OH)2D3- and all-trans retinoic acid-treated HL-60 cells. In addition, ...
... isoquinolinesulfonyl)‐2‐methylpiperazine) (H‐7; Sigma), store for several days at −20°C ... Basic Protocol 2: Harvesting Sea Urchin Gametes. * 20 mM L‐nicotine bitartrate in 100% (v/v) methanol, store in dark up to 1 to ... 72:1‐16. Dinnel, P.A., Link, J.M., Stober, Q.J., Letourneau, M.W., and Roberts, W.F. 1989. Comparative sensitivity of sea ... Zygote 5:277‐287. Slotkin, T.A. 1998. Fetal nicotine or cocaine exposure: Which one is worse? J. Pharmacol. Exp. Ther. 285:931‐ ...
Concepts are listed by decreasing relevance which is based on many factors, including how many publications the person wrote about that topic, how long ago those publications were written, and how many publications other people have written on that same topic ...
Intravitreal injection of liposomes loaded with HA-1077 decreases phospho-profilin 1 in mouse retina.One eye of 9-week-old wild ... The R6/2 HD mouse model is widely used for pre-clinical trials because of its progressive and robust neural dysfunction, which ... The R6/2 HD mouse model is widely used for pre-clinical trials because of its progressive and robust neural dysfunction, which ... Profilin-1 is a Htt binding protein that inhibits Htt aggregation. Its binding to Htt is regulated by the rho-associated kinase ...
5, A and B) and accumulation of vinculin (Fig. 5, D and E) were also associated with the infecting bacteria (Fig. 5, G and H). ... 4, A, B, D, E, G, and H). On the other hand, the CHO cells treated with C3 (at 1.25 μl/ml) for 1 d had an altered cell shape ... A 5 ml culture of YSH6000T in MEM (-FCS) was grown at 37°C up to middle log phase (∼2 × 109 CFU/ml), centrifuged at 4,000 g for ... Figure 2 Enhancement of S. flexneri invasion of CHO cells by Val14RhoA injection. A, C, and E represent serum-starved CHO cells ...
Our results indicate (1) that the ability of various compounds to inhibit PKC in vitro does not correlate with their inhibitory ... Thrombin, 1-oleoyl-2-acetyl-rac-glycerol (OAG), cis- or trans-octadecadienoic acids (linoleic and linolelaidic acid) and the ... Thrombin, 1-oleoyl-2-acetyl-rac-glycerol (OAG), cis- or trans-octadecadienoic acids (linoleic and linolelaidic acid) and the ... 2-methylpiperazine (H-7) or staurosporine, all being potent inhibitors of PKC in vitro. The OAG-induced aggregation, however, ...
The phorbol ester 4 alpha-phorbol 12,13-didecanoate (1-100 nM) did not alter renal vascular resistance, whereas phorbol 12- ... 2 mM) was added to the perfusate. Taken together, our findings support the concept that the renal vasoconstrictory effect of ... 1 microM) and N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide (W-7, 10 microM) but was blunted by staurosporine (100 nM) and ... 1 microM) and N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide (W-7, 10 microM) but was blunted by .... ...
1) The authors show that PKCδ phosphorylates NP to keep it in the monomeric form. As a result this protein is not available for ... 2) The authors speculate that PB2 bridges the binding of PKCδ to NP, however, a direct interaction between PKCδ and PB2 was not ... 1) In Figure 2, panels A, C, and D have bands that are clearly overexposed. While we understand the difficulty in displaying ... 5) Figure 4C. Has the oligomerization status of NP and associated PB2 before and after the kinase assay? This would reveal ...
1999;1:72-74. [PubMed]. *Merrifield CJ, Rescher U, Almers W, Proust J, Gerke V, Sechi AS, Moss SE. Annexin 2 has an essential ... 2000;2:195-205. [PubMed]. *Cramer LP, Mitchison TJ. Myosin is involved in postmitotic cell spreading. J Cell Biol. 1995;131:179 ... In control cells (Figure (Figure5,5, A-H), ST-B-KDEL travels from early/recycling endosomes (Figure (Figure6A)6A) via the ... Figure 2. Electron microscopy of the Golgi complex in untreated (A) and BDM- (40 mM) or ML7 (30 μM)-treated NRK cells (B and C ...
1A), whereas in the presence of 50 μm H2O2, EPSPs were 1.16 ± 0.04 of baseline (n = 12; F = 16.2; p , 0.05). The higher H2O2 ... A, EPSP slopes of 2-month-old tg-SOD mice treated with 50 μm H2O2 and 10 μm H7 or 20 μm FK506. Treatment with H2O2 restores LTP ... H2O2 rescues LTP in tg-SOD slices. We have shown recently (Kamsler and Segal, 2003) that 20 μm H2O2 can inhibit LTP induction ... H2O2 restores LTP in aged wt and inhibits LTP in aged tg-SOD mice. Because aged wt mice are impaired in LTP, as are young tg- ...
... isoquinolinesulfonyl]-2-methyl piperazine (H-7) inhibits the phorbol ester-induced enhancement of bag cell action potentials ... We have now found that treatment of bag cell neurons with the protein kinase inhibitor 1-[5- ...
1 (5 isoquinolinesulfonyl) 2 methylpiperazine. ampicillin. bromocriptine. camptothecin. CD27 antigen. clotrimazole. dasatinib. ... Oncotarget 6 (2) : 814-824. [email protected] Repository. https://doi.org/10.18632/oncotarget.2891. ...
Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00 ... 2-methyl piperazine dihydrochloride, an inhibitor of protein kinase C, had no effect. These results indicate that Ca2+- ...
Y1 - 2001/1. N2 - Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that plays a central role in ... Consistently, an intracellular Ca2+ chelator, BAPTA/AM significantly decreased hypoglycemia-induced VEGF expression and AP-1 ... Consistently, an intracellular Ca2+ chelator, BAPTA/AM significantly decreased hypoglycemia-induced VEGF expression and AP-1 ... Consistently, an intracellular Ca2+ chelator, BAPTA/AM significantly decreased hypoglycemia-induced VEGF expression and AP-1 ...
Second, prolonged exposure of canine carotid arterial rings to PDBu (1-100 nM for 24 hr), a means of depleting PKC from the ... JPET articles become freely available 12 months after publication, and remain freely available for 5 years. Non-open access ... Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00 ... Lowering the concentration of PDBu during preincubation to 30, 10 or 1 nM reduced markedly the inhibitory effects. The inactive ...
2 A and Fig. 3 A), growth cone morphology (Fig. 2 B, Fig. 3 B, and Fig. 5 C), and in retrograde flow and bead diffusion (Fig. 6 ... 2, A and B, Fig. 3, A and B, and Fig. 5 C). These findings support a role for the MIIB isoform in lamellipodial protrusion. ... Figure 1. Pharmacological inhibition using ML-7 leads to a reduction in growth cone area. ML-7 was applied to cultures for a 5- ... 2 B), whereas CALI using a nonspecific IgG had no effect. Loading of MG-anti-MII had no effect on neurite length or growth cone ...
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid. 1. + +. ...
CAS 108930-17-2 - Calbiochem CAS 108930-17-2 A broad-based, cell-permeable, reversible, ATP-competitive serine/threonine kinase ... 2°C to +8°C. Protect from Light. Protect from light. Do not freeze. Ok to freeze. Special Instructions. Following ... 1 mg. Retrieving price.... Price could not be retrieved. Minimum Quantity needs to be mulitiple of Upon Order Completion More ... 2°C to +8°C. Protect from light. Do Not Freeze. Ok to freeze. Special Instructions. Following reconstitution, store in the ...
FIGURE 5 OMITTED] [FIGURE 6 OMITTED] The present study further investigated the potential involvement of muscarinic ... 2-guanidinoethyl)-5-isoquinolinesulfonamide (HA-1004), an inhibitor of PKA (both from RBI, and each at 20 mM in distilled water ... 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein kinase (PK) C, and N-( ...
- Platelet activation by thrombin was only slightly suppressed by polymyxin B, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) or staurosporine, all being potent inhibitors of PKC in vitro. (uni-regensburg.de)
- Using the isolated perfused rat kidney model, we found that renal vasoconstriction produced by ANG II (100 pM) was not altered by the calmodulin antagonists calmidazolium (1 microM) and N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide (W-7, 10 microM) but was blunted by staurosporine (100 nM) and 1-(5-isoquinolinylsulfonyl)-2-methyl-piperazine (H-7, 50 microM), two structurally distinct putative protein kinase C inhibitors. (uni-regensburg.de)
- The phorbol ester 4 alpha-phorbol 12,13-didecanoate (1-100 nM) did not alter renal vascular resistance, whereas phorbol 12-myristate 13-acetate (PMA, 1-100 nM) caused potent and dose-dependent vasoconstriction that was prevented by staurosporine (100 nM) and H-7 (50 microM). (uni-regensburg.de)
- First, the effects of two purported PKC inhibitors, staurosporine (stauro) and H-7 [1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride], were examined on contractility of isolated, intact canine femoral artery. (aspetjournals.org)
- Pretreatment with staurosporine, K252a and H-7 attenuated decreases in 2-DG uptake and CA1 field potentials. (elsevier.com)
- and (b) PMA-induced actin assembly and membrane ruffling were not prevented by the conventional PKC inhibitors 1-(5-isoquinolinesulfonyl)-2-methylpiperazine, staurosporine, calphostin C, or sphingosine at concentrations that precluded PMA-induced protein phosphorylation and superoxide production. (rupress.org)
- Staurosporine and 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine, two structurally different inhibitors of PKC activity, inhibited basal and stimulated EPO production with ED50 values of 9 nM and 50 microM, respectively. (ox.ac.uk)
- 2. A method according to claim 1 , wherein the GAG synthesis inhibitor is/are genistein, NaVO 3 or staurosporine, or combinations thereof. (google.com)
- In addition, inhibitors for protein kinase C [bisindolylmaleimide (GF-109203X), chelerythrine, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7)] and an inhibitor for extracellular signal-regulated kinase [2-(2'-amino-3'-methoxyphenyl)-oxanaphthalen-4-one (PD-098059)] significantly inhibited HL-60 cell differentiation induced by capsaicin in combination with either 1,25-(OH)2D3 or all-trans retinoic acid. (nih.gov)
- 12- O -tetradecanoylphorbol 13-acetate, a phorbol ester that activates protein kinase C, produced no increase in phosphorylation, and 1-(5-isoquinoline sulfonyl)-2-methyl piperazine dihydrochloride, an inhibitor of protein kinase C, had no effect. (diabetesjournals.org)
- 1) Protein kinase inhibitor H-7 [1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride] can stimulate the neurite outgrowth of mouse neuroblastoma N18TG2 cell and NG108-15 hybrid cell. (nii.ac.jp)
- Four groups of rats were infused intra-cranially with 1-(5'-isoquinolinesulfonyl)-2-methylpiperazine (H7-dihydrochloride), a potent inhibitor of both protein kinase C (PKC) and cAMP-dependent protein kinase (PKA), at different time intervals in order to examine their involvement in the acquisition and consolidation of contextual fear memory. (virginia.edu)
- Effects of Phorbol Ester 8- Bromo- cAMP on Bisindolylmaleimide Ι, 2′ - O- dibutiryladenosine- 3′, 5′ - cyclic monophosphate [ ( Bu) 2cAMP], 12- O- tetradecanoyl phorbol- 13- acetate ( TPA), 4α- phorbol 12, Forskolin , N- [ 2- ( p- bromocinnamylamino) ethyl] - 5- isoquinolinesulfonamide dihydrochloride ( H89), 13- didecanoate ( 4α- PDD), forskolin, N6 9- dideoxyforskolin were obtained. (insulintherapie-news.info)
- H-8 dihydrochloride (N-5-isoquinolinesulfonamide. (mybiosource.com)
- Crystal structures of catalytic subunit of cAMP-dependent protein kinase in complex with isoquinolinesulfonyl protein kinase inhibitors H7, H8, and H89. (embl.de)
- Of these, the H series protein kinase inhibitors (1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H7), N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H8) N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89)) are frequently used to block signaling pathways in studies of cellular regulation. (embl.de)
- In future, we will test the inhibitory effect of above described PK inhibitors on both HSV-1 and tumor formation in vivo. (nii.ac.jp)
- Induction of interferon-β (IFN-β) in human (BG-9), simian (CV-1) and mouse (L-929) cell lines by Sendai virus and by poly(rI).poly(rC) has been studied for its possible dependence on protein kinase C (PKC) through the use of pharmacological inhibitors (K252a and H-7) of PKC. (elsevier.com)
- Therefore we examined the effect of protein kinase C inhibitors on oxygen/glucose free-induced impairments of 2-DG uptake and CA1 field potentials. (elsevier.com)
- Human cytomegalovirus stimulates arachidonic acid metabolism through pathways that are affected by inhibitors of phospholipase A2 and protein kinase C . Biochemical and Biophysical Research Communications , 166 (2), 953-959. (utmb.edu)
- AbuBakar, S , Boldogh, I & Albrecht, T 1990, ' Human cytomegalovirus stimulates arachidonic acid metabolism through pathways that are affected by inhibitors of phospholipase A2 and protein kinase C ', Biochemical and Biophysical Research Communications , vol. 166, no. 2, pp. 953-959. (utmb.edu)
- Elicitor-induced production of the phytoalexin, 6-methoxymellein, in cultured carrot cells was appreciably depressed by the calmodulin inhibitors N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide and trifluoperazine. (mysciencework.com)
- PKC activators ( SC- 9 calmodulin inhibitors ( W- 7 , TPA) W- 5) did not block melanophore response to MCH. (insulintherapie-news.info)
- It has been shown that a number of proteins of herpes simplex virus type 1 (HSV- 1) are phosphorylated and HSV-1 encodes viral PKs, but the role of phosphorylation of viral proteins in HSV-1 infection has not been clarified. (nii.ac.jp)
- The results suggest that the inhibitory effect of clonidine on GABA-activated currents in DRG neurons might involve phosphorylation of GABA(A) receptor following activation of α 2 -adrenoceptor through a mechanism of intracellular transduction. (elsevier.com)
- MI significantly increased phosphorylation levels of ERK 42 and ERK 44 by 2-fold and 63% respectively whereas in the fasudil-treated MI group these levels were similar to those in the sham group. (bvsalud.org)
- Differential effects of interleukin-2 and interleukin-4 on protein tyrosine phosphorylation in factor-dependent murine T cells. (naver.com)
- Human promyelocytic leukemia HL-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)2Dor all-trans retinoic acid, respectively. (nih.gov)
- Treatment of HL-60 cells with 5-30 microg/ml capsaicin for 72 h inhibited cell proliferation and induced a small increase in cell differentiation. (nih.gov)
- Capsaicin enhanced protein kinase C activity in 1,25-(OH)2D3- and all-trans retinoic acid-treated HL-60 cells. (nih.gov)
- Uptake of bacteria by the mammalian cells is directed by bacterial factors named IpaB, IpaC, and IpaD invasins, in which Ipa invasins secreted into the bacterial environment can interact with α 5 β 1 integrin. (rupress.org)
- Stimulation of the neurite outgrowth by H-7 was also observed with cerebellar granule cells prepared from 1-week-old mice, the morphology of which was different from that by dB-cAMP. (nii.ac.jp)
- 2) We found that the administration of carbachol, noradrenaline or brdykinin can induce the expression of egr mRNA in NG108-15 cells, which was maximally observed 1 hr after administration. (nii.ac.jp)
- On the other hand, PKC activator 12-o-teradecanoyl phorbol-13-acetate enhanced the HSV-1-induced syncytium formation in A431 epidermoid carcinoma cells, suggesting that PKC activation promotes the development of multinucleated giant cells in herpetic muco-cutaneous lesions. (nii.ac.jp)
- j Immunofluorescent imaging of DMSO or H1152 treated cells for c-peptide expression in 2 different iPSC lines. (nih.gov)
- In contrast, virus-induced IFN production was enhanced threefold or more by K252a in BG-9 and L-929 but not in CV-1 cells. (elsevier.com)
- Viral induction of IFN was not affected significantly by H-7 in BG-9, CV-1 and L-929 cells. (elsevier.com)
- 15. A method of inhibiting the expression of human protein kinase C-α in vitro comprising contacting human cells with a therapeutically effective amount of an antisense oligonucleotide 5 to 50 nucleotides in length, said antisense oligonucleotide comprising a nucleotide sequence complementary to a portion of the sequence set forth in SEQ ID NO: 105. (google.com.au)
- 1. Measurements of membrane capacitance, as an indicator of exocytosis, and intracellular Ca2+ concentration ([Ca2+]i) were used to determine the Ca2+ dependence of secretion in single pancreatic B-cells. (ox.ac.uk)
- During a 72-h incubation, EPO production by the cells was stimulated sevenfold by exposure to low oxygen tension (1%) and threefold by exposure to cobaltous chloride (100 microM). (ox.ac.uk)
- 2. The method of claim 1, wherein the agent inhibits proliferation of epithelial cells in the tissue. (patentsencyclopedia.com)
- 4. The method of claim 1, wherein the internal epithelial cell or tissue are esophageal cells. (patentsencyclopedia.com)
- Cytokines, such as tumor necrosis factor α (TNFα) and interleukin-1 (IL-1) can potentiate the toxic effect of VT by inducing a protein-synthesis dependent increase in VT receptors on endothelial cells. (tudelft.nl)
- Using PRDX6 protein linked to TAT, the transduction domain from human immunodeficiency virus type 1 TAT protein, we demonstrated that PRDX6 was transduced into lens epithelial cells derived from rat or mouse lenses. (courtfield.tk)
- Wortmannin inhibits activation of nuclear transcription factors NF-kappaB and activated protein-1 induced by lipopolysaccharide and phorbol ester. (nih.gov)
- Wortmannin blocked NF-kappaB and AP-1 activation by lipopolysaccharide and phorbol ester but had minimal effect on activation by hydrogen peroxide, ceramide, okadaic acid and tumor necrosis factor. (nih.gov)
- Thus, the mechanism of NF-kappaB and AP-1 activation by lipopolysaccharide and phorbol ester involves PI-3K. (nih.gov)
- Therefore we examined the effect of pretreatment with phorbol ester for 90 min on oxygen/glucose free-induced decreases in 2-DG uptake and CA1 field potentials. (elsevier.com)
- These decrements were not attenuated by 5-min treatment with phorbol ester but were attenuated by 90-min treatment. (elsevier.com)
- The effect of PMA on EPO production was mimicked by phorbol 12,13-dibutyrate (ED50 1 microM) but not by 4 alpha-phorbol 12,13-didecanoate. (ox.ac.uk)
- These results indicate that capsaicin potentiates 1,25-(OH)2D3- or all-trans retinoic acid-induced HL-60 cell differentiation and that both protein kinase C and extracellular signal-regulated kinase are involved in the cell differentiation synergistically enhanced by capsaicin. (nih.gov)
- Thrombin, 1-oleoyl-2-acetyl-rac-glycerol (OAG), cis- or trans-octadecadienoic acids (linoleic and linolelaidic acid) and the synergistic combination of octadecadienoic acids plus OAG lead to the activation of gel-filtered human platelets, i.e. aggregation via protein kinase C (PKC). (uni-regensburg.de)
- On the other hand, actin comet tails were induced by the overexpression of phosphatidylinositol phosphate 5-kinase. (pubmedcentralcanada.ca)
- The crystal structure of the tyrosine kinase domain of fibroblast growth factor receptor 1 (FGFR1K) has been determined in its unliganded form to 2.0 angstroms resolution and in complex with with an ATP analog to 2.3 angstrosms A resolution. (embl.de)
- Fingerprint Dive into the research topics of 'Gene expression of monocyte chemoattractant protein-1 in human monocytes is regulated by cell density through protein tyrosine kinase and protein kinase C'. Together they form a unique fingerprint. (elsevier.com)
- 10. An antisense oligonuclcotide 5 to 50 nucleotides in length comprising a nucleotide sequence which is complementary to the long mRNA transcript of human protein kinase C-α and which is not complementary to the short mRNA transcript of human protein kinase C-α. (google.com.au)
- These results suggest that CMV-induced stimulation of AA metabolism is mediated by pathways which are associated with activation of PLA 2 and protein kinase C. (utmb.edu)
- Specific activity: 1 U/mg (One unit is defined as the amount Protein Kinase G, required to incorporate 1 pmol of phosphate into the substrate peptide kemptide (LRRASLG) in one minute at 25°C using 450 nM cGMP. (proteinkinase.biz)
- The results also indicate that Rho-kinase is central to the ACh-induced stiffness, because inhibition of the kinase by H1152 (1 μM) abolished the stiffening. (ubc.ca)
- The present invention provides a compound of Formula (I) and methods for improving memory, inhibiting rho kinase 1 or 2, inhibiting PIM kinase, or inhibiting IRAK1 kinase in a subject by administering a therapeutically effective amount of the compound. (patentsencyclopedia.com)
- These results demonstrate that the calcium signaling pathway in PBMCs of CKD patients is defective already in CKD stage 2-3, and the pore-forming P2X(7) receptors are involved in these pathophysiological processes. (nih.gov)
- FIGURE 5 OMITTED] [FIGURE 6 OMITTED] The present study further investigated the potential involvement of muscarinic acetylcholine receptors . (acronymfinder.com)
- The induction of egr-1 mRNA expression was abolished by the presence of specific antagonists, indicating that the induction is evoked through specific receptors to stimuli. (nii.ac.jp)
- The shortage of glucose significantly enhanced VEGF mRNA expression in a time-dependent manner as well as increased DNA-binding activity of AP-1 that plays an important role in VEGF transcription. (elsevier.com)
- Therefore, these results indicate that increase of intracellular Ca2+ level induces the activation of PKC, which induce the activation of AP-1 leading to the increase of VEGF in glucose-deprived environment. (elsevier.com)
- We have demonstrated that rat hippocampal slices exposed to oxygen/glucose-free medium showed decreases in 2-deoxyglucose (2-DG) uptake and CA1 field potentials elicited by the stimulation of Schaffer collaterals. (elsevier.com)
- The vasoconstrictory effects of ANG II and PMA were attenuated by the calcium channel blockers verapamil (5 microM) and nifedipine (5 microM) and were reversibly inhibited when cobaltous chloride (2 mM) was added to the perfusate. (uni-regensburg.de)
- Pretreatment of tissues with 1 microM stauro suppressed tension development almost completely when PE and PDBu were the contractile agonists, and partially in K(+)-depolarized rings. (aspetjournals.org)
- The synthetic diacylglycerol analogues oleolyl-acetylglycerol and dioctanoylglycerol (2-200 microM) also had no effect on either basal or stimulated EPO production. (ox.ac.uk)
- Genistein and prunetin which inhibit PTK inhibited the replication of HSV-1 at genistein concentrations of more than 25muM,whereas the related comp … More ounds, which do not inhibit PTK,did not affect the replication of HSV-1. (nii.ac.jp)
- Human prostate cancer cell lines PC3 and DU145 were treated with fasudil at the concentrations of 5, 10, 20, 40, 80, and 160 μmol/L, respectively, and those as negative controls cultured in the Ham's-F12 medium, all for 24 hours. (bvsalud.org)
- Hydrogen peroxide (H 2 O 2 ), a reactive oxygen species, is assumed to have a detrimental effect on neuronal plasticity. (jneurosci.org)
- Metabolic products of oxidation/reduction processes such as hydrogen peroxide (H 2 O 2 ) have been considered, until recently, only in the context of the homeostatic maintenance of cellular functions. (jneurosci.org)
- Lowering the concentration of PDBu during preincubation to 30, 10 or 1 nM reduced markedly the inhibitory effects. (aspetjournals.org)
- Publications] Yoshiaki Yura: 'Inhibitory effect of tyrphostin on the replication of herpes simplex virus type 1' Archives of Virology. (nii.ac.jp)
- Then, MTT assay was used to measure the cell inhibition rate and half maximal inhibitory concentration (IC50) value of fasudil, with 1/4 of IC50 as the medication dose for further investigation. (bvsalud.org)
- 2001 May 25;420(2-3):83-90. (nih.gov)
- 2001 Jan;7(1):91-96. (elsevier.com)
- We now use hippocampal slices from tg-SOD mice and evoked LTP by theta burst stimulation (TBS), which is patterned after innate rhythms in the hippocampus and is a milder stimulation that does not saturate the LTP generating mechanism ( Morgan and Teyler, 2001 ), to further investigate the role of H 2 O 2 as a diffuse modulator of synaptic plasticity. (jneurosci.org)
- HA-1077 increased photopic and flicker ERG response amplitudes in R6/2 mice, but not in wild-type littermate controls. (nih.gov)
- Intravitreal injection of liposomes loaded with HA-1077 decreases phospho-profilin 1 in mouse retina.One eye of 9-week-old wild-type mice (n = 4) was intravitreally injected with liposomes loaded with 40 µM HA-1077, and the other eye was intravitreally injected with empty liposomes. (nih.gov)
- Indeed, H 2 O 2 suppresses long-term potentiation (LTP) in hippocampal slices of normal rats and wild-type (wt) mice. (jneurosci.org)