The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
Moreover, the dynamic of H3K27me3 at PCH during in vitro conversion from naïve to primed pluripotent state and during ESCs derivation suggests that the mechanisms underlying the control of this histone mark at PCH are different in embryo and in vitro. (sciencegate.app)
Currently, the ability to culture in vitro totipotent cells possessing molecular and functional features like those of an early embryo in vivo has been a challenge. (sciencegate.app)
This study underscores the subtle differences between in vitro derived TBLCs and in vivo mouse early developmental cell stages at the single-cell transcriptomic level. (sciencegate.app)
2- to 4-cell and morula- to blastocyst-stage mouse embryos were cultured for 1 h in tritiated leucine at two specific activities and their subsequent development followed in vitro and in vivo (after transfer to recipients), respectively. (sciencegate.app)
Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro. (caltech.edu)
Differentiation1
After implantation differentiation of trophoblast large cells which get excited about the remodeling from the maternal uterus during implantation is certainly achieved through many endocycles resulting in boosts of DNA articles up to 1000N (48). (bioerc-iend.org)
Post implantation8
Furthermore, bioactive surfaces (PL and BIO) showed functional mechanical stability (resonance frequency analyses) as early as 4 weeks post implantation via increased total bone area (BAT=56% and 59%) ingrowth compared to SB (BAT=35%) and AE (BAT=35%) surfaces. (academic-accelerator.com)
In the present paper, blood vessel formation and maturation were studied within and around implanted micro-structured type-I collagen scaffolds at 10 weeks post implantation in adult rat mid-cervical spinal cord lateral funiculotomy injuries. (academic-accelerator.com)
The spatiotemporal distribution of pro-inflammatory (CD68+CD86+TNF-α+) and pro-remodeling (CD68+CD206+) macrophages, and the remodeling response in terms of vascularization, total number of infiltrating cells, presence of multinucleate giant cells (MNGC), and cell layer thickness around the implanted materials was evaluated at 3, 7, 14, 21, and 35 days post implantation. (academic-accelerator.com)
Scaffolds were harvested 21 days post implantation and taken for histologic analysis to characterize the cellular infiltrate in response to the primary tumor. (academic-accelerator.com)
Over 70% of eyes in both groups became medication-free by 6 months post implantation. (academic-accelerator.com)
Auditory and speech performance results were compared across both subject groups using the Category of Auditory Performance (CAP) and Speech Intelligibility Rate (SIR) tests, with measurements recorded one year post implantation. (academic-accelerator.com)
0) questionnaires were used to assess the hearing-related quality of life in both groups at 1 year post implantation. (academic-accelerator.com)
Gastrulation1
Embryo model completes gastrulation to neurulation and organogenesis. (caltech.edu)
Extra embryonic1
Modeling human embryo development with embryonic and extra-embryonic stem cells. (caltech.edu)
Embryonic cells1
Remarkably, a subpopulation within the TBLCs population expressed a high level of the totipotent-related genes Zscan4s and displayed transcriptomic features similar to mouse two-cell stage embryonic cells. (sciencegate.app)
Embryogenesis1
To facilitate a quantitative morphological analysis of early mouse development under controlled conditions, a perfusion culture system capable of supporting embryogenesis to blastocyst stage has been developed. (sciencegate.app)
However, it is disputed whether TBLCs are 'true' totipotent stem cells equivalent to in vivo two-cell stage embryos. (sciencegate.app)
Methylation3
At blastocyst stage, the segregation of the three primordial lineages is accompanied by establishment of differential patterns of DNA methylation and post-translational modifications of histones, such as H3K27me3. (sciencegate.app)
Notably, this region included a Zfp57 binding site that is essential for maintaining paternal methylation during the early stages of pre-implantation 5 , 6 . (nature.com)
Somatic DNA methylation patterns that can persist throughout life are established shortly after fertilisation when the majority of epigenetic marks, including DNA methylation, are erased from the pre-implantation embryo. (frontiersin.org)
Early2
Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
To address this question, single-cell RNA sequencing was applied to TBLCs and cells from early mouse embryonic developmental stages and the data were integrated using canonical correlation analyses. (sciencegate.app)
Blastocyst1
In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
Through the definition of a coordinate system based on mesh structure and the development of a special sectioning procedure, sections can be localized within the intact embryo and three-dimensional coordinates given to any element of embryo volume. (sciencegate.app)
Alternatively in endocycles DNA replication (the S stage) and cell development take place lacking any intervening mitosis (M stage) phase resulting in polyploidy. (bioerc-iend.org)
Analyses1
Differential expression analyses were performed between TBLCs and multi-embryonic cell stages to identify differentially expressed genes. (sciencegate.app)
Cell7
Quantitative evaluation of tissue-processing procedures has permitted selection of conditions which reduce changes in linear dimensions to −1·6 ± 1·8 % in two-cell embryos. (sciencegate.app)
Used jointly these total outcomes claim that can be an important regulator from the cell routine in the preimplantation embryo. (bioerc-iend.org)
through all stages from the cell routine may be thoroughly modified during advancement to be able to meet the demands of a given Dalcetrapib cell at specific stages. (bioerc-iend.org)
On the two-cell stage transcription from the zygotic genome takes place and is necessary for the next cleavages to occur (11). (bioerc-iend.org)
Generation of Stem Cell-Based Mouse Embryo-Like Structures. (caltech.edu)
Stem-cell-based human and mouse embryo models. (caltech.edu)
Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension. (caltech.edu)
Culture1
The use of a mesh system allows identification of individual embryos by position, and control of their orientation during culture and preparation for light and electron microscopy. (sciencegate.app)
Distribution1
We show that this histone modification is first enriched at PCH in the whole embryo and evolves into a diffuse distribution in epiblast during its specification and maturation. (sciencegate.app)