Zinc dna binding proteins. Medical search. Frequent questions

Exons 1-6 of the WT1 gene encode the regulatory domain, which regulates expression of target genes, and exons 7-10 encode the 4 zinc fingers of the DNA-binding region of the WT1 protein. (medscape.com)
C2H2-type (classical) zinc fingers (Znf) were the first class to be characterised. (embl.de)
Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)
Znf domains are often found in clusters, where fingers can have different binding specificities. (embl.de)
The first of the targetable cleavage reagents to be developed were the zinc-finger nucleases (ZFNs) (1), which consist of a non-specific cleavage domain from a restriction endonuclease linked to a DNArecognition domain made up of zinc fingers. (ddw-online.com)
Each finger contacts primarily three base pairs of DNA, and there are natural and derived fingers that recognise many of the 64 possible triplets. (ddw-online.com)
It takes two ZFN monomers to make a break, each of which carries three to six fingers chosen to bind the desired genomic target (Table 1). (ddw-online.com)
The requirement for a total of 6-12 fingers, each of which binds three base pairs, provides enough specificity to address a unique sequence in a genome as complex as the three billion base pairs of human DNA. (ddw-online.com)
A simpler alternative to zinc fingers emerged with the characterisation of transcription activator-like effectors (TALEs) in plant pathogenic bacteria of the genus Xanthomonas. (ddw-online.com)
We have performed comprehensive analysis of homeodomain and Cys2His2 zinc finger specificities in D. melanogaster - (fruit fly) in collaboration with Michael Brodsky (UMMS-MCCB) & Gary Stormo (Wash. U). We have coupled this data with specificity data from artificial zinc fingers and homeodomains that we have selected using our bacterial one-hybrid system. (umassmed.edu)
This dataset can also be used to broadly predict the specificity of homeodomains ( http://stormo.wustl.edu/flyhd/ ) and zinc fingers ( http://stormo.wustl.edu/ZFModels/ ). (umassmed.edu)
DNAbinding proteins combine with DNA through several motifs called zinc fingers, zinc twists, ring fingers, or zinc clusters. (dojindo.com)
LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. (thermofisher.com)
Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. (thermofisher.com)
The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)
C) Enlargement of part of the DBD showing the amino acid sequence (single letter codes) of the 2 zinc fingers and the dimerization loop (in bold). (medscape.com)
The process of TZD binding to the cognate DNA is likely stepwise: initially TZD non-specifically binds to DNA, then fingers 1 and 2 insert cooperatively into the major groove of DNA by semi-specific binding, and finally finger 3 non-specifically binds to DNA, which promotes the specific binding on fingers 1 and 2 and stabilizes the formation of a specific TZD-DNA complex. (sinica.edu.tw)
This role involves its DNA-binding domain, which is composed of a tandem array of zinc fingers, and PRDM9-dependent trimethylation of histone H3 at lysine 4. (springer.com)

We report here that SRE1, a gene encoding a GATA-type protein, bound to promoter sequences of genes involved in siderophore biosynthesis. (nih.gov)
Our data rule out a model suggesting that xKaiso is a direct repressor of Wnt signalling target genes in early Xenopus development via binding to promoter-proximal CTGCNA sequences as part of a xTcf3 repressor complex. (biologists.com)
The new tools that have opened the door to facile manipulation of genomic DNA sequences are enzymes that can be designed to cut DNA specifically at essentially any arbitrarily chosen sequence. (ddw-online.com)
To study the interactions between these sequences and regulatory factors, HeLa cell nuclear proteins were analyzed by band-shift assay using a 95-base pair (bp) DNA probe containing a part of the human MT-IIA gene upstream sequences. (go.jp)
namely, this protein is activated only by Zn, and requires not only MRE but also its flanking sequences for optimal DNA-binding. (go.jp)
The other protein appears to be Spl, based on its recognition sequences specificity. (go.jp)
We isolated 29 unique ZFP sequences of which zinc proliferation 1 (Zipro1) was the most abundant. (tamu.edu)
Parallel-stranded DNA with natural base sequences. (mpg.de)
The DNA-binding domain (DBD) of the hGRα corresponds to amino acids 420-480 and contains 2 zinc finger motifs through which the hGRα binds to specific DNA sequences, the glucocorticoid-response elements (GREs) in the promoter region(s) of target genes. (medscape.com)
12/15 shared amino acids) and the protein sequences have 70%-82% homology. (cdc.gov)
Amino acid position is in a conserved region compared to other Aspergilli (26/31 shared amino acids) and the protein sequences have 84% homology. (cdc.gov)

Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. (nih.gov)
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)
They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. (embl.de)
LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. (thermofisher.com)
We created several deletion mutants of this protein based on its conserved motifs, and from these structures functional chimeras were synthesized, supporting the hypothesis that gene duplication and recombination could lead to novel functional forms of single-stranded DNA-binding proteins. (johnshopkins.edu)
DEAF-1 recognizes several TTCG motifs within the portion of the Dfd autoregulatory region termed 'module E.' In addition, DEAF-1 binds several similar motifs within a Dfd response element ( DRE ) from the 1.28 gene that enhances maxillary gene expression during embryogenesis (Pederson, 2000). (sdbonline.org)
The 2 zinc finger motifs are able to tetrahedrally coordinate a zinc atom and are held by 4 cysteine (Cys) residues (see the image below, panel C). (medscape.com)

The loss of iron led to a approximately 2.5-fold decrease in DNA-binding affinity, indicating that iron was directly involved in SRE1 regulation of iron-uptake genes. (nih.gov)
The WT1 protein mediates the mesenchymal-epithelial transition and differentiation during morphogenesis of the kidney and gonad by repressing genes that encode cell proliferation factors and by activating genes that encode markers of epithelial cell differentiation. (medscape.com)
We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
Our data show that the stable knockdown of HIPK2 led to wtp53 misfolding, as detected by p53 immunoprecipitation with conformation-specific antibodies, and that p53 protein misfolding impaired p53 DNA binding and transcription of target genes. (aacrjournals.org)
We present evidence that zinc supplementation to HIPK2i cells increased p53 reactivity to conformation-sensitive PAb1620 (wild-type conformation) antibody and restored p53 sequence-specific DNA binding in vivo and transcription of target genes in response to Adriamycin treatment. (aacrjournals.org)
Most unexpectedly, genes coding for similar proteins were found in the genomes of eukaryotes, including humans. (johnshopkins.edu)
At the cellular level, the actions of glucocorticoids are mediated by a 94-kd protein, the glucocorticoid receptor (GR). The human (h) GR belongs to the steroid/thyroid/retinoic acid superfamily of nuclear receptors and functions as a ligand-dependent transcription factor that regulates the expression of glucocorticoid-responsive genes positively or negatively. (medscape.com)

This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. (nih.gov)
Using this information we can build simple qualitative models of recognition that allow the design of homeodomains with novel DNA-binding specificity. (umassmed.edu)
E3 ubiquitin-protein ligases determine the substrate specificity for ubiquitylation and have been classified into HECT and RING-finger families. (embl-heidelberg.de)
2012 ), generalizing the observation that hotspot localization is determined by the DNA-binding specificity of the zinc finger array (Grey et al. (springer.com)

This gene encodes a member of the Kruppel family of zinc finger proteins. (wikipedia.org)
The Snail gene family encodes DNA-binding zinc finger proteins that function as transcriptional repressors. (plos.org)
Background: This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. (idhinhibitor.com)
Ortholog in C. albicans is ENP2 , encodes an protein. (cdc.gov)

Mutations in the conserved cysteine residues in the cysteine-rich region led to a decrease in bound iron. (nih.gov)
They contain a short beta hairpin and an alpha helix (beta/beta/alpha structure), where a single zinc atom is held in place by Cys(2)His(2) (C2H2) residues in a tetrahedral array. (embl.de)
In the proposed "zinc finger" DNA-binding motif, each repeat unit binds a zinc metal ion through invariant Cys and His residues and this drives the folding of each 30-residue unit into an independent nucleic acid-binding domain. (embl.de)

An experimentally determined model of the single finger is proposed that is consistent with circular dichroism, one- and two-dimensional nuclear magnetic resonance, and visual spectroscopy of the single-finger peptide reconstituted in the presence of zinc. (embl.de)
In addition, by South-western blotting analysis of nuclear extracts using the 95-bp probe or MRE oligonucleotide probe, we detected a Zn-dependent DNA-binding protein with a molecular mass of 116 kDa, which is likely to be ZRF. (go.jp)
Analysis of HeLa cell nuclear proteins fractionated by glycerol gradient centrifugation showed that ZRF is distinct from another MRE-binding protein, MREBP. (go.jp)
Risk and nonrisk oligonucleotides were tested for binding of nuclear extracts from A549, BEAS-2B, and IMR-90 lung cell lines by using electrophoretic mobility shift assays. (cdc.gov)
Electrophoretic mobility shift assays detected allele-dependent nuclear protein binding in A549 cells for 8 of 21 variants. (cdc.gov)
Has cohesin ATPase activity, double-stranded DNA binding, topological DNA entrapment activity, role in mitotic cohesin loading and condensed nuclear chromosome, centromeric region, nuclear mitotic cohesin complex localization. (cdc.gov)

Normally, zinc attaches to four sulfur atoms, causing the protein to adopt a structure that binds to DNA. (stanford.edu)
mechanics (QM), in particular the Schr ¨odinger equation, allow us different proteins, e.g. alcohol dehydrogenase, blue copper proteins, to calculate any measurable property of a defined set of atoms. (lu.se)

The molecular basis for the role of zinc in developmental biology. (ncl.edu.tw)
It functions as a molecular chaperone, aiding the refolding of non-native proteins, and plays a critical role in stabilisation of the cytoskeleton through interactions with several cytoskeletal components, such as actin, intermediate filaments and microtubules [ 7 ]. (biomedcentral.com)
We combine structure-based protein engineering and molecular genetics to restrict the activity of the potentially toxic endonuclease to spermatogenesis. (nature.com)
Protein structures are invaluable for biochemical studies and for mechanical or molecular mechanics calculations have become the understanding of how proteins work. (lu.se)

Targeting a DNA binding motif of the EVI1 protein by a pyrrole-imidazole polyamide. (rochester.edu)
SELEX assays and footprinting data indicate that DEAF-1 binds to and activates Mtk and Drs regulatory DNAs via a TTCGGBT motif. (sdbonline.org)
The 3D structure of the zinc ligation system is unique to the RING domain and is referred to as the 'cross-brace' motif. (embl-heidelberg.de)

The interaction of Kaiso with xTcf3 is highly conserved and is dependent on its zinc-finger domains (ZF1-3) and the corresponding HMG DNA-binding domain of TCF3/4 factors. (biologists.com)

Moreover, these long noncoding RNAs were transcriptionally regulated by epigenetic modification, including DNA methylation and histone methylation modification. (cancerindex.org)
Cross-talk between different histone modifications, as well as DNA methylation, seems complex. (aacrjournals.org)
In mammalian cells, histone H3 Lys 9 (H3K9) methylation and histone hypoacetylation are usually associated with methylated DNA, heterochromatin, and gene silencing. (aacrjournals.org)
Histone hyperacetylation and H3K4 methylation are associated with unmethylated DNA, euchromatin, and gene expression ( Fig. 2 ). (aacrjournals.org)
The effects of DNA methylation and the histone code are due, at least in part, to modification-specific recruitment of factors, such as heterochromatin-associated proteins (HP1) and methyl-binding domain proteins, which establish and maintain higher order of chromatin structure. (aacrjournals.org)
2010 ). PRDM9 includes several functional domains, such as a DNA-binding zinc finger array, a histone lysine methyltransferase PR/SET (PRDI-BF1 and RIZ1 homology) domain, and a Krüppel-associated box (KRAB)-related domain. (springer.com)

In the auxin and jasmonic acid systems, ligand recognition is shared between protein-binding partners. (nature.com)
The human GR is a modular protein composed of distinct regions illustrated in panel B in the image below, as follows: (1) The amino-terminal A/B region, also called immunogenic or N-terminal domain (NTD) and (2) the C, D, and E regions, which correspond to the DNA-binding domain, the hinge region, and the ligand-binding domain, respectively. (medscape.com)
The spacing of the cysteines in such a domain is C-x(2)-C-x(9 to 39)-C-x(1 to 3)-H-x(2 to 3)-C-x(2)-C-x(4 to 48)-C-x(2)-C. Metal ligand pairs one and three co-ordinate to bind one zinc ion, whilst pairs two and four bind the second. (embl-heidelberg.de)

This binding sites where shown within 5.0 Angstroms radius around Zinc atom. (atomistry.com)
Many gene expression proteins contain a zinc atom that can be replaced by a lead atom if a person is exposed to too much lead. (stanford.edu)
However, the direction of shift perturbation, change of DNA imino-proton NMR signal, and dynamics on the 15 N backbone atom significantly differed between specific and non-specific binding. (sinica.edu.tw)

Photophysics of green and red fluorescent proteins: Implications for quantitative microscopy. (mpg.de)

The AF-1 plays an important role in the interaction of the receptor with molecules necessary for the initiation of transcription, such as coactivators, chromatin modulators, and basal transcription factors, including RNA polymerase II, TATA-binding protein (TBP), and a host of TBP-associated proteins (TAFIIs). (medscape.com)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)
DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)

We found that after CST transection, levels for Zipro1 mRNA doubled and that Zipro1 protein expression increased in non-myelinating CST SCs. (tamu.edu)
Alternative splicing of the primary transcript gives rise to the 2 mRNA and protein isoforms, hGR-alpha and hGR-beta. (medscape.com)
The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. (idhinhibitor.com)

UV-binding factors found in 12-h-old zebrafish embryos may be involved in processing developmental stage-specific DNA structures similar to UV-damage DNA. (ncl.edu.tw)
The functional domains and subdomains are indicated beneath the linearized protein structures. (medscape.com)
Such calculations can also be used to interpret the structures, e.g. to decide the protonation state of metal-bound ligands. (lu.se)
The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)

Deformed epidermal autoregulatory factor-1 (DEAF-1) is a transcription factor that was originally shown to bind the autoregulatory enhancer of the Deformed ( Dfd ) Hox gene, which is activated in embryonic head segments of Drosophila (Gross, 1996). (sdbonline.org)

It may represent an accumulation of misfolded, damaged or unwanted proteins, awaiting or undergoing degradation. (biomedcentral.com)
Ubiquitination targets proteins for degradation and is a potent regulator of cellular protein function. (embl-heidelberg.de)
Furthermore, nutlin-3a-induced PARP1 degradation enhanced DNA-damaging effects of cisplatin in BRCA1 knockdown cells. (oncotarget.com)
Our study revealed that nutlin-3a is a PARP1 suppressor that induces PARP1 proteasomal degradation by binding to MDM2 and promoting autoPARylation of PARP1. (oncotarget.com)

The Crystal Structure of the Herpes Simplex Virus 1 Ssdna-Binding Protein Suggests the Structural Basis For Flexible, Cooperative Single-Stranded Dna Binding. (atomistry.com)
The architecture of single-stranded DNA-binding proteins, which play key roles in DNA metabolism, is based on different combinations of the oligonucleotide/oligosaccharide binding (OB) fold. (johnshopkins.edu)
Here we show that unlike these groups of organisms, the Euryarchaeota has exploited the potential in the OB fold to re-invent single-stranded DNA-binding proteins many times. (johnshopkins.edu)
Most interestingly, a second single-stranded DNA-binding protein, FacRPA2, in this archaeon exhibited the wrapping mode. (johnshopkins.edu)
Although the diversity shown by archaeal single-stranded DNA-binding proteins is unparalleled, the presence of their simplest form in many organisms across all domains of life is of greater evolutionary consequence. (johnshopkins.edu)
The protein has a preference for binding single stranded DNA and RNA. (idhinhibitor.com)

They have proved somewhat difficult to engineer for new targets, however, because an individual finger may not bind its triplet well in all possible sequence contexts. (ddw-online.com)

CYP2A6 is a protein that can be found in liver cells, where it is one of many enzymes responsible for removing toxic chemicals from the body. (stanford.edu)
Because they are membrane-bound proteins, this class of enzymes has been particularly difficult to work with. (stanford.edu)
Zinc ion regulates the activities of several enzymes. (dojindo.com)
These enzymes modify histones, which are structural proteins that attach (bind) to DNA and give chromosomes their shape. (medlineplus.gov)

Sre1p specifically bound DNA containing the 5'-(G/A)ATC(T/A)GATAA-3' sequence, and that binding was both iron- and zinc-dependent. (nih.gov)
Thus, a targeted break in DNA can lead to localised mutations or engineered sequence changes. (ddw-online.com)
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. (nih.gov)
The others are the site-specific TFs or the DNA sequence-specific binding proteins. (intechopen.com)
Although genetics have played a dominant role in cancer research, epigenetics (heritable changes in gene function that do not involve alterations in DNA sequence) has become equally important in this field. (aacrjournals.org)
20%-21% protein sequence homology. (cdc.gov)

Members of this DNA-binding protein family act as transcriptional regulators. (wikipedia.org)
EVI1 Interferes with Myeloid Maturation via Transcriptional Repression of Cebpa, via Binding to Two Far Downstream Regulatory Elements. (rochester.edu)
DDB, a putative DNA repair protein, can function as a transcriptional partner of E2F1. (ncl.edu.tw)

Homeodomain-interacting protein kinase-2 (HIPK2) is a positive regulator of p53 oncosuppressor function. (aacrjournals.org)

KIN17, which is known as a DNA and RNA binding protein, is highly expressed in numerous types of human cancers and was discovered to participate in several vital cell behaviors, including DNA replication, damage repair, regulation of cell cycle and RNA processing. (spandidos-publications.com)
Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)
It participates in the regulation of cellular PH and is blocked by zinc. (lu.se)

Dojindo offers a wide selection of fluorescent intracellular ion probes for calcium, proton, zinc, and chloride ions. (dojindo.com)
Calcium indicators selectively bind to calcium ions. (dojindo.com)
This means that a deprotonation step is involved in the calcium binding process, which causes a change in the fluorescent signal. (dojindo.com)
In designing calcium probes, the BAPTA structure is required as the calcium-binding moiety. (dojindo.com)
If the calcium ion binds to indicators, donation of the lone electron pair on the aromatic nitrogen to the calcium ion causes a change in the fluorescent signaling. (dojindo.com)
Blood lead was significantly higher in the malignant case croups, as were zinc, iron, and calcium. (who.int)

PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)
In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)

DNA constructs were cloned into a pGL3 promoter vector for luciferase gene reporter assays. (cdc.gov)

PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)

Zinc finger protein 165 is a protein that in humans is encoded by the ZNF165 gene. (wikipedia.org)
Zinc is an essential metal ion, and its level is reported to be 28.5 mg per 1 g tissue in humans. (dojindo.com)
In humans and mice, hotspots are specified by PRDM9, a meiosis-specific PRDM family protein (Baudat et al. (springer.com)

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. (lu.se)

We are in the midst of a revolution in genome engineering, based on reagents that can be designed to cut chromosomal DNA at arbitrary sites. (ddw-online.com)
Creating more precise CRISPR/Cas9 nucleases for gene therapy - We are utilzing our expertise in protein engineering and protein-DNA recognition to create hybrid CRISPR/Cas9 nucleases to achieve single-site cleavage precision within the human genome. (umassmed.edu)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

However, since most zinc ions are strongly combined with proteins, the concentration of free zinc ions in a cell is at the millimolar level or lower. (dojindo.com)
A shortage of zinc ions influences DNA transcription, and may have some relationship with the development of cancer. (dojindo.com)
Furthermore, zinc ions are thought to have a role in controlling gestation and sexual activity. (dojindo.com)
Some tissues and organs, such as the brain, spermatazoa, and salivary glands, contain a large amount of zinc ions. (dojindo.com)
Staining of such tissues with TSQ has demonstrated that zinc ions play very important roles in neuronal cell death, endocrine functions, and other physiological phenomena. (dojindo.com)
The role of intracellular zinc ions in apoptosis has also been discussed for several decades. (dojindo.com)

Identification and isolation of damage-recognition protein complexes from zebrafish(Danio rerio) early embryos. (ncl.edu.tw)
Unlike the embryonic extract, the extracts of 84-h-old larvae produced only low-shifting binding complexes when incubated with a CPD-specific probe. (ncl.edu.tw)

Using affinity chromatography coupled to multidimensional protein identification technology (MudPIT), DEAF-1 was identified as a candidate regulator. (sdbonline.org)
DNA affinity precipitation and mass spectroscopy of rs147978008 revealed allele-dependent binding of H1 histones, which was confirmed by using Western blotting. (cdc.gov)

Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

Stem cell antigen-1 (Sca-1) is a member of the Ly-6 multigene family encoding highly homologous, glycosyl-phosphatidylinositol-anchored membrane proteins. (plos.org)

First, the general TFs (GTFs), including preinitiation complex components TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and THIIH, are the primary protein factors that are required for the initiation of transcription from the TATA box (or TATA element), then elongation is executed by RNA polymerase II (RNA pol II) [ 1 ]. (intechopen.com)
Here, we used the rat cervical sympathetic trunk (CST), an autonomic nerve in which non-myelinating SCs are the predominant cell type, to isolate zinc-finger protein (ZFP) cDNAs by reverse transcriptase-polymerase chain reaction. (tamu.edu)
Poly (ADP-ribose) polymerase 1 (PARP1) plays important roles in single strand DNA repair. (oncotarget.com)

We demonstrate that a direct interaction between the methyl-CpG-dependent transcription repressor Kaiso and xTcf3, a transducer of the Wnt signalling pathway, results in their mutual disengagement from their respective DNA-binding sites. (biologists.com)
To gain insights into the interaction mechanism, here we further investigated the DNA-binding characteristics of TZD bound to non-specific DNAs and its finger segments bound to cognate DNA. (sinica.edu.tw)
The RING domain is a protein interaction domain that has been implicated in a range of diverse biological processes. (embl-heidelberg.de)
In S. cerevisiae , this interaction is at least in part provided by Spp1 that directly interacts with both methylated H3K4 near DSB sites and the axis-localized protein Mer2 (Acquaviva et al. (springer.com)

DNA recognition in these proteins is provided by modules, each of which binds a single base pair, and there is a robust code to address each of the four base pairs (2,3). (ddw-online.com)
The binding of 12-hr-old and 84-hr-old extracts to CPD- and 6-4PPs under various concentration of NaCl supported the possibility that different damage-recognition protein were expressed in zebrafish according to their developmental condition. (ncl.edu.tw)
The binding activity expressed in embryos was apparently unrelated to human DNA repair damage-recognition proteins XPA、RPA-70 and RPA-32, since those polypeptides recognized by an anti-human XPA、RPA-70 and RPA-32 antibody were detected only in 84-h-old zebrafish extracts. (ncl.edu.tw)
The DNA damage-recognition problem in human and other eukaryotic cell: the XPA damage binding protein. (ncl.edu.tw)
Characterization of DNA recognition by the human UV-damaged DNA-binding protein. (ncl.edu.tw)

Identification of one 50.2 and one 29-kDa UV-damage-DNA binding factor in 12-hr-old zebrafish extracts by EMSA linked to SDS-PAGE and determined by MALDI TOF analysis, indicated that the 29-kDa and 50.2-kDa polypeptides are high homologous to zebrafish fast skeletal muscle troponin T and vitellogenin. (ncl.edu.tw)

The first described CID systems involve microbe-derived dimerizing ligands, such as rapamycin, that direct protein neo-associations that rewire eukaryotic cell signaling 5 . (nature.com)

Scope includes mutations and abnormal protein expression. (cancerindex.org)

AFUA_2G16480 domain(s) with predicted zinc ion binding activity. (cdc.gov)

Instead, we propose that mutual inhibition by Kaiso/TCF3 of their DNA-binding functions may be important in developmental or cancer contexts and acts as a regulatory node that integrates epigenetic and Wnt signalling pathways. (biologists.com)

To obtain structural information, we synthesized single and double zinc finger peptides from the yeast transcription activator ADR1, and assessed the metal-binding and DNA-binding properties of these peptides, as well as the solution structure of the metal-stabilized domains, with the use of a variety of spectroscopic techniques. (embl.de)

A fifth variant (FAM71A rs147978008) showed nonrisk allele preferential binding to H1 histones. (cdc.gov)

Esmaeili R, Zhang M, Sternberg MR, Mapango C, Pfeiffer CM . A new multiplex immunoassay for serum ferritin, soluble transferrin receptor, C-reactive protein, α-1-glycoprotein, and retinol-binding protein showed variable comparability with traditional reference assays external icon . (cdc.gov)

We therefore hypothesized that the selective induction of DNA double-stranded breaks in the X chromosome during male meiosis might be a potential strategy to distort the sex ratio of the progeny towards male mosquitoes, if the normal production of Y-bearing gametes was unaffected ( Fig. 1a ). (nature.com)

There are 2594488 ZnF_C2H2 domains in 441262 proteins in SMART's nrdb database. (embl.de)
The 576-aa DEAF-1 protein possesses two conserved domains, SAND and MYND. (sdbonline.org)
This entry represents RING-type zinc finger domains. (embl-heidelberg.de)
There are 308772 RING domains in 276130 proteins in SMART's nrdb database. (embl-heidelberg.de)
The MG132 proteasome inhibitor, and knockdown of checkpoint with forkhead and ring finger domains (CHFR) and ring finger protein 146 (RNF146), E3 ubiquitin ligases targeting PARP1, suppressed nutlin-3a-induced PARP1 reduction. (oncotarget.com)

Anatomy13

Organisms22

Diseases1

Chemicals and Drugs119

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes75

Information Science3

Health Care2

Fingers18

Sequences11

Motifs8

Genes8

Specificity4

Encodes4

Residues3

Nuclear6

Atoms2

Molecular4

Motif3

Highly conserved1

Histone6

Ligand3

Atom3

Fluorescent Proteins1

Chromatin3

MRNA3

Structures4

19961

Degradation4

Single-stranded6

Targets1

Enzymes4

Sequence6

Transcriptional3

Kinase1

Regulation3

Calcium6

KRAB2

Promoter1

Localization1

Humans3

Membrane-bound1

Genome3

Ions6

Complexes2

Affinity2

Nuclease1

Multigene1

Polymerase3

Interaction4

Recognition5

Polypeptides1

Eukaryotic cell1

Mutations1

AFUA1

Regulatory1

Structural1

Histones1

Assays1

Double-stranded1

Domains5