Zebrafish skeletal myogenesis satellite cells skeletal muscle. Medical search. Frequent questions

In zebrafish, Myf5 is the first MRF expressed in embryonic myogenesis and is required for adult viability, even though larval muscle forms normally. (wikipedia.org)

As one of the key cell markers of satellite cells (the stem cell pool for skeletal muscles), it plays an important role in the regeneration of adult muscle. (wikipedia.org)
Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
Established models of injury have used myotoxins and physical trauma to demonstrate the concurrence of myogenesis and angiogenesis during regeneration. (biomedcentral.com)
It is unknown whether the spatiotemporal coupling between myofiber and microvascular regeneration persists when muscle tissue is completely removed and local guidance cues are lost. (biomedcentral.com)
To test whether complete removal of skeletal muscle tissue affects the spatiotemporal relationship between myogenesis and angiogenesis during regeneration, subthreshold volumetric muscle loss was created with a biopsy punch (diameter, 2 mm) through the center of the gluteus maximus (GM) in adult mice. (biomedcentral.com)
Following punch biopsy of adult skeletal muscle, regenerating microvessels span the wound and become perfused with blood prior to myofiber regeneration. (biomedcentral.com)
We conclude that angiogenesis precedes myogenesis during regeneration following subthreshold volumetric muscle loss. (biomedcentral.com)
Therefore, as an alternative approach to test the interdependence of microvascular and myofiber regeneration following acute injury, a punch biopsy (diameter, 2 mm) was performed through the mouse gluteus maximus (GM) muscle. (biomedcentral.com)
Skeletal muscle satellite tv for pc cells (SCs) are stem cells chargeable for muscle improvement and regeneration. (korilog.com)
Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
In particular, in skeletal muscle, specific semaphorin molecules are involved in the restoration and remodeling of the nerve-muscle connections, thus emphasizing their plausible role to ensure the success of muscle regeneration. (biomed.news)
This review article aims to discuss the impact of aging on skeletal muscle regeneration and NMJs remodeling and will highlight the most recent insights about the role of semaphorins in this context. (biomed.news)
Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)

Although Myf5 is mainly associated with myogenesis, it is expressed in other tissues, as well. (wikipedia.org)
In this chapter, we describe the methodology for analyzing macrophages in various tissues in the adult zebrafish by flow cytometry. (bvsalud.org)
Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. (u-paris.fr)
In this study, we present a fully automatic multi-atlas segmentation pipeline we have developed for segmentation of quadriceps muscles from magnetic resonance images obtained in twenty five young healthy males.Materials and Methods: A non-linear registration process based on ANTs algorithm was applied to thigh images initially automatically segmented for bone, muscle and fat tissues. (jain-foundation.org)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)

Some evidence shows that Myf5 and MyoD are responsible for the development of separate muscle lineages, and are not expressed concurrently in the same cell. (wikipedia.org)
Still, the existence of these separate "MyoD-dependent" and "Myf5-dependent" subpopulations has been disputed, with some claiming that these MRFs are indeed coexpressed in muscle progenitor cells. (wikipedia.org)
As no muscle is formed in Myf5;Myod double mutant zebrafish, Myf5 cooperates with Myod to promote myogenesis. (wikipedia.org)
The MyoD gene was duplicated during the teleost whole genome duplication and, while a second MyoD gene ( MyoD2 ) was subsequently lost from the genomes of some lineages (including zebrafish), many fish lineages (including Alcolapia species) have retained both MyoD paralogues. (mdpi.com)
All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)

Most notably, while Myf5 is activated by Sonic hedgehog in the epaxial lineage, it is instead directly activated by the transcription factor Pax3 in hypaxial cells. (wikipedia.org)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)

It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. (wikipedia.org)
Although it is expressed in both the epaxial (to become muscles of the back) and hypaxial (body wall and limb muscles) portions of the myotome, it is regulated differently in these tissue lines, providing part of their alternative differentiation. (wikipedia.org)

Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)

Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)

Although the specific roles and dependency of Myf5 in adipogenesis and neurogenesis have remained to be explored, these findings show that Myf5 may play roles outside of myogenesis. (wikipedia.org)
This webinar included overviews of the clinical phenotype and genetics of dysferlinopathy, and also highlighted some of the findings of recent clinical studies, in particular the Clinical Outcome Study in Dysferlinopathy (COS), as well as large-scale sequencing studies that document the spectrum of mutations of the DYSF gene and carry implications about the role of the dysferlin protein in muscle biology. (jain-foundation.org)
This webinar included findings from metabolic studies on dysferlin-deficient muscle fibers, and the impact of dietary interventions on the phenotype of dysferlinopathy. (jain-foundation.org)

While the product of this gene is capable of directing cells towards the skeletal muscle lineage, it is not absolutely required for this process. (wikipedia.org)
They expressed macrophage-associated genes, but also showed decreased phagocytic gene expression and increased epithelial-associated gene expression, characteristic of metaphocytes, recently discovered ectoderm-derived cells. (bvsalud.org)
Using Affymetrix Gene Chip analysis, we have identified genes linked to regenerative success downstream of BMP signalling, including the BMP inhibitor Gremlin and the stress protein Hsp60 ( no blastema in zebrafish). (biomedcentral.com)

Despite only being present for a few days during embryonic development, Myf5 is still expressed in certain adult cells. (wikipedia.org)

We further demonstrated that juvenile csf1r-deficient zebrafish exhibit systemic macrophage depletion. (bvsalud.org)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)

Specifically, it allows a brief pulse of proliferation of these satellite cells in response to injury. (wikipedia.org)

Tissue macrophages are essential components of the immune system that also play key roles in vertebrate development and homeostasis, including in zebrafish, which has gained popularity over the years as a translational model for human disease. (bvsalud.org)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)

however, priority needs to be given to resistance exercise training, and secondarily to adequate protein intake should be part of this dietary regime to mitigate losing muscle mass. (biomed.news)

In contrast to preserving residual guidance cues following myotoxins or physical trauma, volumetric muscle loss (VML) removes all tissue components. (biomedcentral.com)
Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)

Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)

The IAL aims to synergise the expertise in muscle and neuromuscular diseases of French teams and the expertise in immunology and inflammation of the Brazilian teams . (institut-myologie.org)

7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)

Front Cell Dev Biol. (u-paris.fr)

Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)

For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)

Here, we analyzed mpeg1:GFP-expressing macrophages in csf1r-deficient zebrafish and report that embryonic macrophages emerge followed by their developmental arrest. (bvsalud.org)

It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)

3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

Furthermore, Myf5 is a master regulator of muscle development, possessing the ability to induce a muscle phenotype upon its forced expression in fibroblastic cells. (wikipedia.org)
The high redundancy of this system shows how crucial the development of skeletal muscle is to the viability of the fetus. (wikipedia.org)
Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)

Acute injury to skeletal muscle damages myofibers and fragment capillaries, impairing contractile function and local perfusion. (biomedcentral.com)

This activity, developed with the full support of the AFM as well as the Dutch Duchenne Parent Project NL (DPP-NL) and the Jain Foundation, has resulted in the creation of a platform for the immortalisation of human cells. (institut-myologie.org)

In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

These studies were performed after it was shown that Myf5 knockouts had no clear abnormality in their skeletal muscle. (wikipedia.org)
Identification of Lgals3bpb as a new pan-microglia marker will be fundamental in forthcoming studies using the zebrafish model. (bvsalud.org)

The absence of all three of these factors results in a phenotype with no skeletal muscle. (wikipedia.org)
Validation and reproducibility of the pipeline was assessed on two other databases of seven healthy male subjects.Results: Results for each quadriceps muscle show a mean DICE similarity coefficient higher than 0.85.Conclusion:Through some examples, we show the robustness of this registration method for the longitudinal follow-up of atypical healthy subjects and dytrophic patients. (jain-foundation.org)
This procedure results in rotation of the globe toward the resected muscle, increased resting tension across the agonist-antagonist pair, and stretching of the elastic components of the muscles. (arvojournals.org)
In the rabbit, due to orbital constraints and limited rotation, resection results in more significant stretch of the surgically treated muscle than the antagonist. (arvojournals.org)

A provisional matrix filled with PDGFRα + and CD45 + cells spanned the wound within 1 dpi. (biomedcentral.com)
Myogenesis into the wound was apparent by 14 dpi and traversed the wound by 21 dpi. (biomedcentral.com)

Primary MuSCs isolated from individual skeletal muscles (gastrocnemius and masseter) are lysed, and their RNA is reverse-transcribed into cDNA and copied into double-stranded DNA. (biomed.news)

Data from crosslinking and analysis of cDNAs (CRAC) of Nab3 in yeast cells expressing a circular ncRNA decoy. (u-paris.fr)
Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)

In larvae, mpeg1+ cell numbers then increased showing two distinct types in the skin: branched, putative Langerhans cells, and amoeboid cells. (bvsalud.org)
Metabolic changes in skeletal muscle are a hallmark of several types of muscular dystrophy. (jain-foundation.org)

BACKGROUND: Two decades ago, the fish-specific monoclonal antibody 4C4 was found to be highly reactive to zebrafish microglia, the macrophages of the central nervous system. (bvsalud.org)

This expression is eventually repressed to prevent extraneous muscle formation. (wikipedia.org)
Commonly, zebrafish macrophages are identified based on expression of fluorescent transgenic reporters, allowing for real-time imaging in living animals. (bvsalud.org)
In addition, whether the 4C4 expression pattern is strictly restricted to microglial cells in zebrafish has never been investigated. (bvsalud.org)

In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

However, no study has used this method so far for skeletal muscle segmentation. (jain-foundation.org)

Overall, we aim at providing a guide for the zebrafish community based on our expertise investigating the adult mononuclear phagocyte system. (bvsalud.org)

In contrast, although numbers also increased in csf1r-mutants, exclusively amoeboid mpeg1+ cells were present, which we showed by genetic lineage tracing to have a non-hematopoietic origin. (bvsalud.org)

However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)

Zebrafish deficient for csf1r are viable and permit analyzing the consequences of macrophage loss throughout life. (bvsalud.org)

These cells are regulated by a tight control of transcriptional programs. (biomed.news)
The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)

Optimized fusion parameters of STEPS multi-atlas segmentation were determined to obtain the highest DICE index as compared to the manual segmentation of quadriceps muscles, considered as the gold standard. (jain-foundation.org)

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