• We identified 1149 differentially expressed genes, which were separated into two major modules by weighted gene correlation network analysis, one consisting of mostly upregulated genes correlated with regeneration and the other consisting of only downregulated genes associated with metabolism and homeostatic processes. (biomedcentral.com)
  • This type of regeneration is rare among vertebrates but does occur in the African clawed frog Xenopus laevis , traditionally a model organism for the study of early development. (biomedcentral.com)
  • While all vertebrates are capable of some types of tissue regeneration, most, including humans, have lost the ability to regenerate whole structures such as limbs ( epimorphic regeneration ), [ 1 ]. (biomedcentral.com)
  • This study demonstrates the importance of a stress response for stem cell activation and regeneration in a basal chordate, which may have implications for understanding the limited regenerative activities in other animals, including vertebrates. (biomedcentral.com)
  • In vertebrates with limited regeneration capacities, such as mammals, wound repair typically terminates in fibrotic scar formation and is not followed by regeneration. (biomedcentral.com)
  • Tunicate embryogenesis is highly determinative: deletion of blastomeres or larval parts does not result in their replacement [ 13 ]. (biomedcentral.com)
  • In our study, we investigated whether the deletion of miR-378a (miR-378) affects muscle regeneration. (bvsalud.org)
  • To conclude, our study revealed that miR-378 deletion influences the abundance of mSCs and the adipogenic potential of FAPs, but does not affect overall regeneration upon acute, glycerol-induced muscle injury. (bvsalud.org)
  • Unidirectional regeneration in the basal chordate Ciona intestinalis involves the proliferation of adult stem cells residing in the branchial sac vasculature and the migration of progenitor cells to the site of distal injury. (biomedcentral.com)
  • However, after the Ciona body is bisected, regeneration occurs in the proximal but not in the distal fragments, even if the latter include a part of the branchial sac with stem cells. (biomedcentral.com)
  • How adult muscle stem cells contribute to this decrease in regenerative capacity is incompletely understood. (bvsalud.org)
  • Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)
  • Regeneration-competent wound healing of amphibian appendages is generally rapid and involves covering the wound surface with a specialised epidermis lacking a basement membrane and dermis [ 3 , 4 ]. (biomedcentral.com)
  • We have used this line to show that BMP function is not only required for appendage regeneration but that it is specifically needed to generate a proliferating blastema while being dispensable for wound healing [ 12 ]. (biomedcentral.com)
  • In animals with higher regeneration capacities, wound repair and regeneration are linked, but the molecular relationship between these two processes is unclear. (biomedcentral.com)
  • The findings reveal the presence of highly heterogeneous cell populations within the callus, including epidermis, vascular tissue, shoot primordia, inner callus, and outgrowth shoots. (bvsalud.org)
  • The molecular basis of the shoot regeneration process in crop callus remains largely elusive. (bvsalud.org)
  • These findings significantly advance our understanding of the cellular and molecular aspects of shoot regeneration in tomato callus and demonstrate the immense potential of spatial transcriptomics in plant biology. (bvsalud.org)
  • siRNA-mediated gene knockdown showed that hsp70 and dnaJb4, but not bag3, are required for progenitor cell targeting and distal regeneration. (biomedcentral.com)
  • Heat shock treatment of distal body fragments activated hsp70 and dnaJb4 expression indicative of a stress response, induced cell proliferation in branchial sac vasculature cells, and promoted distal regeneration. (biomedcentral.com)
  • The stress response is absent from distal fragments, but can be induced by a heat shock, which activates cell division in the branchial sac vasculature and promotes distal regeneration. (biomedcentral.com)
  • Callus is a reprogrammed cell mass involved in plant regeneration and gene transformation in crop engineering. (bvsalud.org)
  • Light is shown to promote shoot regeneration by inducing chlorenchyma cell development and coordinating sugar signaling. (bvsalud.org)
  • Muscle regeneration was induced using intramuscular cardiotoxin injection or treadmill exercise and analysed using single cell and bulk RNA sequencing, qRT-PCR and immunofluorescence. (bvsalud.org)
  • Using Affymetrix Gene Chip analysis, we have identified genes linked to regenerative success downstream of BMP signalling, including the BMP inhibitor Gremlin and the stress protein Hsp60 ( no blastema in zebrafish). (biomedcentral.com)
  • Gene Ontology analysis showed that genes involved in embryonic development and growth are significantly over-represented in regenerating early hindlimb buds and that successful regeneration in the Xenopus hindlimb correlates with the induction of stress response pathways. (biomedcentral.com)
  • We have developed a heat shock inducible transgenic line ( N1 ) of Xenopus in which the BMP antagonist Noggin can be induced at a specific time during either development or regeneration, repressing BMP signalling [ 10 , 12 ]. (biomedcentral.com)
  • This study pioneers the exploration of the spatial transcriptome of tomato callus during shoot regeneration. (bvsalud.org)
  • Notably, chlorenchyma cells, enriched in photosynthesis-related processes, play a crucial role in promoting shoot primordia formation and subsequent shoot regeneration. (bvsalud.org)
  • The chaperone system genes hsp70 , dnaJb4 , and bag3 are significantly upregulated in the branchial sac vasculature following distal injury, defining a stress response that is essential for regeneration. (biomedcentral.com)
  • 3 fold at stationary phase compared to the early mid log phase. (gp120-inhibitors.com)
  • However, the mRNA that includes Inhibitors,Modulators,Libraries the intergenic region showed variation de pending on the stage of growth, increasing 20 fold at stationary phase compared with its expression at early mid log phase. (gp120-inhibitors.com)
  • Adult zebrafish fins fully regenerate after resection, providing a highly accessible and remarkable vertebrate model of organ regeneration. (bvsalud.org)
  • The zebrafish is a powerful in vivo vertebrate model system for exploring cellular processes during development and thus may help to elucidate mechanisms of DomA developmental neurotoxicity. (nih.gov)