Loading...
  • chikungunya
  • A study performed at the University of São Paulo's Biomedical Science Institute (ICB-USP) in Brazil shows that sofosbuvir, a drug used to treat chronic hepatitis C, is capable of eliminating chikungunya virus and yellow fever virus. (eurekalert.org)
  • Human cells infected by chikungunya virus were treated with sofosbuvir, and the drug eliminated the virus without damaging any cells. (eurekalert.org)
  • immunization
  • The World Health Organization now pushes for child immunization programs which will slowly decrease yellow fever cases as well as other fatal diseases that were once a major problem. (adobe.com)
  • detection
  • Reported methods for the detection of the yellow fever viral genome are beset by limitations in sensitivity, specificity, strain detection spectra, and suitability to laboratories with simple infrastructure in areas of endemicity. (asm.org)
  • We describe the development of two different approaches affording sensitive and specific detection of the yellow fever genome: a real-time reverse transcription-quantitative PCR (RT-qPCR) and an isothermal protocol employing the same primer-probe set but based on helicase-dependent amplification technology (RT-tHDA). (asm.org)
  • Unbiased PCR amplification of total RNA extracted directly from patient sera and next generation sequencing resulted in detection of yellow fever virus and generation of 98% of the virus genome sequence. (semanticscholar.org)
  • Yellow fever virus: genetic and phenotypic diversity and implications for detection, prevention and therapy. (semanticscholar.org)
  • chills
  • PATHOGENICITY/TOXICITY: It is estimated that 15 to 50 % of people infected with YF develop illness, beginning abruptly with fever, chills and headache(5). (msdsonline.com)
  • Real-tim
  • The RT-qPCR assay is a powerful tool for reference or diagnostic laboratories with real-time PCR capability, while the isothermal RT-tHDA assay represents a useful alternative to earlier amplification techniques for the molecular diagnosis of yellow fever by field or point-of-care laboratories. (asm.org)
  • antiviral
  • Yellow fever is among one of the most lethal viral diseases for which approved antiviral therapies were yet to be discovered. (springer.com)
  • Thus, the 10 lead molecules suggested in the present study would be of interest as promising starting point for designing antiviral compound against yellow fever. (springer.com)
  • phenotypic
  • The data suggest that uncloned yellow fever virus populations, passaged a limited number of times, and exhibiting some phenotypic changes, are representative of the original virus strain and can be used with a reasonable degree of confidence in vector competence studies. (ajtmh.org)
  • assays
  • Both assays were evaluated using yellow fever cell culture supernatants as well as spiked and clinical samples. (asm.org)
  • antibody
  • The subcutaneous inoculation of the culture virus into immune persons leads to a substantial increase of the serum antibody titer. (rupress.org)
  • Trinidad
  • Thus, sequencing Venezuelan YFV strains over a wide geographic area and temporal distribution might be valuable to test the hypothesis of local maintenance in Venezuela and to determine whether the virus moves regularly between Trinidad and Venezuela or between Venezuela and Brazil. (thefreelibrary.com)