• cDNA
  • A cDNA library (complementary DNA) contains DNA prepared from the mRNA present in a given cell population using the enzymes reverse transcriptase , which produces single-stranded DNA from mRNA, and DNA polymerase, which converts single-stranded DNA into double-stranded DNA. (le.ac.uk)
  • As higher quality sources of sequence verified cloned cDNA have become available, this practice has become less urgent. (wikipedia.org)
  • 1987
  • The first description of what would later be called CRISPR was from Osaka University researcher Yoshizumi Ishino in 1987, who accidentally cloned part of a CRISPR together with the iap gene, the target of interest. (wikipedia.org)