Begin by inoculating a large swab of yeast cells into a flask at least four times the volume of the culture containing 50 milliliters of standard YP medium supplemented with 2%raffinose and 0.1%glucose as a carbon source and grow the culture overnight in a 30 degree Celsius shaker. (jove.com)
The next morning, inoculate the 50 milliliter pre-culture in one liter of standard YP medium with 2%raffinose and 0.1%glucose in a 2.8 liter volume baffled Fernbach flask for a four to five-hour culture in the shaking incubator. (jove.com)
Introduction of heterologous glucose- or fructose-proton symporters in an hxt 0 yeast background strain (derived from CEN.PK2-1C) restored growth on the corresponding sugar under aerobic conditions. (biomedcentral.com)
In such environments, the high extracellular glucose concentration can drive the uptake of glucose by yeast cells via diffusion, which is exemplified by the occurrence of many hexose transporters with a facilitated diffusion mechanism in S. cerevisiae . (biomedcentral.com)
Qianwa reported that 1.5˜2.0 mg/l of recombinant human lactoferrin was obtained by expressing it in the form of a fusion protein between a yeast invertase and human lactoferrin by using a chelatin promoter in Saccharomyces cerevisiae (Qianwa Liang et.al. (justia.com)
In the yeast Saccharomyces cerevisiae , which is widely applied for industrial bioethanol production, uptake of hexoses is mediated by transporters with a facilitated diffusion mechanism. (biomedcentral.com)
The yeast thallus (vegetative body) in its simplest form is a single cell or perhaps one with a bud still attached as exemplified by Saccharomyces cerevisiae. (biologydiscussion.com)
The yeast strain AK 46 was isolated from dough containing fermented cherry fruits and identified as Saccharomyces cerevisiae. (embrapa.br)
Species6
Naganishia antarctica is a yeast species that has been isolated from soil in Antarctica. (wikipedia.org)
Yeasts are eukaryotic microorganisms, classified in the kingdom fungi, with about 1500 species currently identified and described. (scirp.org)
The study aimed at isolation and identification of yeast species in palm sap (palm wine), to ascertain their ability to degrade petroleum hydrocarbons. (scirp.org)
Chromgenic Listeria Agar Basewith Listeria Selective Supplement ( A Selective And Differential Agar Medium For Rapid And Direct Identification Of Listeria Species. (haryanatenders.net)
Identification tests are standardized to enable investigators in different laboratories to compare their cultures with standard descriptions of type species and varieties. (biologydiscussion.com)
Sucrose2
This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. (bvsalud.org)
The S. mutans samples are processed and inoculated in selective culture media, such as mitis salivarius agar with bacitracin (MSB) and trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) 15 . (bvsalud.org)
Xylose1
Only certain strains of N. anarctica can use citrate at pH 6.0, D-glucitol, L-arabinose, raffinose and xylose as sole carbon sources. (wikipedia.org)
Numerical analysis and computer identification of Hansenula, Pichia and related yeast genera. (microbiologyresearch.org)
Taxonomic consideration of the yeasts relies heavily on morphological characteristics for genera. (biologydiscussion.com)
Strain2
In this study, we performed mutagenic analyses of the C-terminal region of the catalytic core domain of HIV-1 IN in order to delineate the critical amino acid(s) and/or motif(s) required for the induction of the lethal phenotype in the yeast strain HP16, and to further elucidate the molecular mechanism which causes this phenotype. (biomedcentral.com)
The nucleotide sequence of the rDNA spacer region from this strain coincided with those from the yeasts used for the production of whisky, wine, and bread. (embrapa.br)
Morphological1
1) Isolate and characterize yeasts from palm wine using morphological and molecular method. (scirp.org)
Fungi5
GPI biosynthesis is a conserved process, required for anchoring proteins to the plasma membrane of fungi and thus essential for the integrity of the fungal cell wall in yeasts and molds 4 . (nature.com)
9] reported that palm wine is a suspension of different types of microorganisms including bacteria, filamentous fungi, and yeasts. (scirp.org)
At present, many taxonomists are in agreement with the arrangement proposed by Ainsworth in which the fungi are treated as a separate kingdom and the yeasts are included in the division Eumycota. (biologydiscussion.com)
While the definition of yeasts varies somewhat according to author, they are generally defined as fungi which, in a stage of their life cycle, occur as single cells, reproducing commonly by budding or less frequently by fission. (biologydiscussion.com)
The distinction of yeasts from related mycelial fungal forms is highly subjective, resulting in a number of transitional forms between yeasts and the more typical higher fungi. (biologydiscussion.com)
Extract1
Both ethyl acetate extract and alkaline ethyl acetate extract were tested for phytochemical content by qualitative analysis and evaluated for their biological activities such as antimicrobial (by agar well diffusion assay and minimum inhibitory concentration determination) and cytotoxic activities (by MTT assay in cell culture). (biomedres.info)
Molds1
Diverse microbial population bacteria, yeasts or molds have been reported for degradation of hydrocarbons. (scirp.org)
Vegetative2
In budding yeast, individual chromosomes from vegetative cells cannot be visualized by microscopy. (silverchair.com)
Vegetative reproduction by fission is characteristic In this article we will discuss about the yeast:- 1. (biologydiscussion.com)
Acetic2
Yeast single and double mutants lacking RTG2 and/or certain factors regulating carbon source utilization, including MIG1 , HXK2 , ADR1 , CAT8 , and HAP4 , have been analyzed for their survival and CIT2 expression after acetic acid treatment. (microbialcell.com)
Moreover, our data show that simultaneous mitochondrial retrograde pathway activation and SNF1 -dependent relief of CCR have a key role in central carbon metabolism reprogramming which modulates the yeast acetic acid-stress response. (microbialcell.com)
Broth2
Hydrocarbon utilizing yeasts (HUY) were isolated from palm wine, a rich sugar substrate from a non-oil impacted environment using Bacto Bushnell Haas broth. (scirp.org)
Yeast isolates were further screened for hydrocarbon degrading potentials using the Bacto Bushnell Haas broth containing 2, 6-dichlorophenol indophenols as the indicator agent. (scirp.org)
Belong1
Botanists of the 19th century generally accepted the idea that yeasts belong to the plant kingdom. (biologydiscussion.com)
Mutants2
Our study identified three HIV-1 IN mutants, V165A, A179P and KR186,7AA, located in the C-terminal region of the catalytic core domain of IN that do not induce the lethal phenotype in yeast. (biomedcentral.com)
Chromatin binding assays in yeast and mammalian cells demonstrated that these IN mutants were impaired for the ability to bind chromatin. (biomedcentral.com)
Chromogenic Mueller Hinton Agar ( For Differentiation Of Organisms Based On Chromogenic Differentiation And Determination Of Susceptibility Of Microorganisms Against Antimicrobial Agents ) , Chromogenic Mueller Hinton Agar ( For Differentiation Of Organisms Based On Chromogenic Differentiation And Determination Of Susceptibility Of Microorganisms Against Antimicrobial Agents ) , Chromogenic Mueller Hinton Agar For Antifungal Testing ( For The Chromogenic Differentiation Of Yeast From Clinical Samples And Determination Of Susceptibility To Antifungal Agents. (haryanatenders.net)
Widely1
Yeasts are widely dispersed in the environment. (scirp.org)
Concentration1
When the optical density at 600 nanometers reaches 0.2, add galactose to a final concentration of 2%to the cells for an overnight culture in the shaking incubator. (jove.com)
500Ml1
r.biopharma 56 adrenaline hydrochloride 500ml 57 af media 100ml bottle ( ready to use, media should contains fetal bovine serum ( fbs ) , gentamicin, and l glutamine. (jharkhandtenders.net)
Methods2
Our methods can be used to understand the lipid specificities of all yeast SNX-BARs or to produce recombinant SNX-BAR proteins from any other organism. (jove.com)
In order to facilitate the isolation of this microorganism selective Gold culture media have been used 13 , and different methods have been proposed in an attempt to simplify S. mutans culture in laboratory for its detection and quantification 14 . (bvsalud.org)
Isolation1
The study aimed at isolation, characterization and testing of yeasts from palm wine for biodegradation of petroleum hydrocarbons. (scirp.org)
Yield1
In anaerobic cultures, a higher ethanol yield can be achieved when transport of hexoses is proton-coupled, because of the lower net ATP yield of sugar dissimilation. (biomedcentral.com)
Cells4
Purifying the SNB-BAR proteins from yeast cells allows the acquisition of native homo and hetero SNX-BAR dimers while minimizing the toxicity and insolubility frequently encountered with non-native expression systems. (jove.com)
The next morning, harvest cells by centrifugation and transfer the yeast pellet into a 50 milliliter conical tube. (jove.com)
In vitro, CM from HUVECs drastically greater order LDE225 the proliferation of MHCC97H cells, and induced increased expression of MMP2, MMP9, OPN, and CD44 in contrast with the manage medium. (fak-signal.com)
The early investigators described yeasts as being round to oval in appear-ance and noted that they divided by budding to form daughter cells. (biologydiscussion.com)
Reproduction2
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (webmedcentral.com)
These two encoded genes were up-regulated during early infection, and the encoded proteins were confirmed to be secreted using a yeast secretion system. (biomedcentral.com)
Sugar2
The emergence of Crabtree-positive yeasts 100-150 million years ago probably coincided with the emergence of fruit-bearing plants, providing a sugar-rich niche in which these yeasts evolved [ 9 , 10 ]. (biomedcentral.com)
Take for example en.wikipedia.org/wiki/Raffinose , that's a sugar that can't be digested by you. (stackexchange.com)
Source1
This is a good thing too because it looks like home brewers can't get a homofermentative Lactobacillus from an easily available yeast lab source. (beeradvocate.com)
Dynamics1
Starter culture growth dynamics and sensorial properties of fermented oat drink. (tftak.eu)
Liquid1
When being cultured in liquid media, constant agitation is required. (wikipedia.org)
Nature1
ADR1 and CAT8 interact with RTG2 and with each other in inducing cell resistance to AA-PCD in raffinose and controlling the nature of cell death. (microbialcell.com)
Lactic acid bacteria are generally fastidious on artificial media, but they grow readily in most food substrates and lower the pH rapidly to a point where competing organisms are no longer able to grow. (nzdl.org)
To prove that point, I performed an experiment at Omega Yeast Labs to address the question. (beeradvocate.com)
Industrial terms such as "cultured, true, wild, top, or bottom yeasts" have little meaning from the botanical point of view. (biologydiscussion.com)
70% apparent attenuation) 100% Lactobacillus fermentations are not possible and those reported as such were in reality a result of unintentionally introducing yeast. (beeradvocate.com)
Higher2
Nucleic acid having higher density finds place as 'core', medium denser protein in intermediate and least dense fat in the periphery. (webmedcentral.com)
Yeasts lack chlorophyll and are unable to manufacture by photosynthesis from inorganic substrates the organic compounds required for growth, as do higher plants, algae, and even some bacteria. (biologydiscussion.com)
Produce2
The ability to form sexual spores within an ascus or produce them externally on a basidium places most yeasts in the subdivisions Ascomycotina and Basidiomycotina, respectively. (biologydiscussion.com)
There are yeasts, however, in which the buds will remain attached so that the mother cell and first daughter cell may produce additional buds. (biologydiscussion.com)
Method1
The present invention relates to a method of mass-producing lactoferrin polypeptides from yeast which is resistant to lactoferrin polypeptides. (justia.com)
Region2
Overall, this study demonstrates that three mutations located in the C-terminal region of the catalytic core domain of HIV-1 IN inhibit the IN-induced lethal phenotype in yeast by inhibiting the binding of IN to the host chromatin. (biomedcentral.com)
These results demonstrate that the C-terminal region of the catalytic core domain of HIV-1 IN is important for binding to host chromatin and is crucial for both viral replication and the promotion of the IN-induced lethal phenotype in yeast. (biomedcentral.com)
Dosing regimens can be designed based on population statistics, using MIC 90 (e.g., packaged inserts) as a surrogate indicator of what is needed, or using MIC data from a culture report. (paperzz.com)