Yeast artificial chromosomes artificial gene fusion. Medical search. Frequent questions

New vector for transfer of yeast artificial chromosomes to mammalian cells. (ox.ac.uk)
A modification vector has been constructed to facilitate the transfer of yeast artificial chromosomes (YACs) to mammalian cells in culture by targeting a dominant selectable marker (G418 resistance) to the right arm of pYAC4 clones. (ox.ac.uk)
DNA is microinfected into the fertilized egg A. after the fusion of male and female nuclei B. before the fusion of male and female nuclei C. at the time of fusion of male and female nuclei D. any time, it can be infected In which year, Dolly the first mammalian clone was born? (vnedu.org)
This research is a breakthrough in bioengineering technology, helping to understand the impact of large-scale remodeling of mammalian chromosomes, and to gain a deeper understanding of the molecular mechanisms underlying growth and development, reproductive evolution, and even species formation. (seculartalk.net)
According to scientists, their breakthrough could lead to treatments for conditions such as infertility and diseases such as cancer, allowing researchers to monitor and control chromosomes in mammalian cells. (seculartalk.net)

So you can separate chromosomes based on size. (nih.gov)
Gorillas have two separate chromosomes whereas in humans they are fused, and a translocation between ancestor human chromosomes produced two different chromosomes in gorillas. (eurekalert.org)

Its genome contains one genome copy from the S. uvarum parental genome and two heterozygous copies of the S. cerevisiae parental genome, with the exception of a monosomic S. cerevisiae chromosome III, where the sex-determining MAT locus is located. (frontiersin.org)
Twenty years ago genetic studies were based not on disease but on chromosomes, because it was so hard to study a whole genome. (nih.gov)
A genome, which is the totality of the organism's genes, can either be subjected to genetics or genomics - noting the comparative difference between them. (cannabistech.com)
therefore, inferring that the genes required to form these are peculiar to the cannabis genome. (cannabistech.com)
Advancements in cannabis genomics brought to light the reason behind the transformation of a hemp plant into a marijuana plant - further analyses of a cannabis genome showed millions of DNA codons discretely separated into ten chromosomes, with all having similar characteristics but chromosome 6, which held both synthases ("THC synthase" and "CBD synthase"), separated by about 20 million nucleotides. (cannabistech.com)
The sre Gene( ORF469) has a Site-Specific Recombinase Responsible for Integration of the R4 Phage Genome, 1996 J. Transgene approximating by the online artificial photosynthesis receptor functionality: substances for the cytoplasm of several IntechOpen data in promoters and bacteria, 2000 Plant Mol. (scoutconnection.com)
Telomeres are nucleoprotein structures at the end of chromosomes that preserve the stability and function of the genome. (crcm-marseille.fr)
Recent advances in microarray technology allow for the quantification, on a single array, of transcript levels for every known gene in the human genome. (wikidoc.org)
Because it is very difficult to clone a GFP-fused protein into its native location in the genome, however, this method often cannot be used to measure endogenous regulatory mechanisms (GFP-fusions are therefore most often expressed on extra-genomic DNA such as an expression vector ). (wikidoc.org)

The genes/mutations could act alone or interact with other genetic and/or epigenetic or environmental factors, causing autism or related disorders. (neurotransmitter.net)
The first stage involved identifying, in unrelated subjects showing linkage to 2q24-q33, genetic variants in exons and flanking sequence within candidate genes and comparing the frequency of the variants between autistic and unrelated nonautistic subjects. (neurotransmitter.net)
Evolutionary chromosomal changes may take a million years in nature, but researchers are now reporting a novel technique enabling programmable chromosome fusion that has successfully produced mice with genetic changes that occur on a million-year evolutionary scale in the laboratory. (eurekalert.org)
While the consistent reliability of the chromosomes is good for understanding how things works on a short-time scale, the ability to engineer changes could inform genetic understanding across millennia, including how to correct misaligned or malformed chromosomes, LI said. (eurekalert.org)
Genomic imprinting is frequently lost, meaning the information about which genes should be active disappears, in haploid embryonic stem cells, limiting their pluripotency and genetic engineering," WANG said. (eurekalert.org)
To resolve misconfigured cells we include a Bayesian fitted online artificial that is a different restriction to perform the genetic and the yeast genes, Qbeing P-splines. (scoutconnection.com)
Interestingly, cytoplasmic genetic elements have been shown to have very different inheritance patterns to classic Mendelian nuclear chromosomes. (nature.com)
The laboratory is part of a consortium that aims to identify genes responsible for telomere biology disordes, rare genetic diseases caused by premature telomere shortening. (crcm-marseille.fr)
A number of scientific papers have been published indicating that LSD might cause structural changes in the chromosomes, genetic mutations, disturbances of embryonic development, and malignant degeneration of cells. (druglibrary.net)
The genetic controversy about LSD started in 1967 when Cohen, Marinello and Back (22) published a paper suggesting that LSD should be added to the list of substances capable of causing abnormalities in the chromosomes. (druglibrary.net)

This vector has been tested by modification of a 550-kb YAC containing part of the human MHC class II region and transfer to CHO cells by protoplast fusion. (ox.ac.uk)
The main part deals with examples of modern FISH-applications, highlighting unique possibilities of the approach, like the possibility to study individual cells and even individual chromosomes. (frontiersin.org)
According to co-first author WANG Libin, researcher with CAS and the Beijing Institute for Stem Cell and Regenerative Medicine, the difficulty is that the process requires deriving stem cells from unfertilized mouse embryos, meaning the cells only contain one set of chromosomes. (eurekalert.org)
In diploid cells, there are two sets of chromosomes that align and negotiate the genetics of the resulting organism. (eurekalert.org)
Gaussian online artificial photosynthesis in Human Cells Catalyzed by Phage polynucleotide Integrase Mutants, 2000 J. Maeser and Kahmann, The Gin model of event Mu can promote supernatant health in spline pairs, 1991 Mol. (scoutconnection.com)
As the fermentation for the ethanol production is over, the cells are separated to get the biomass of yeast cells that is used A. for next fermentation B. as single cell protein for animal feed C. as manure D. all of the above. (vnedu.org)
Second, host cells can be transformed with the genes that code for the rare tRNAs. (hideyuki-ts.com)
Typically and traditionally, the gene of interest (GOI) is incorporated into a plasmid vector via a ligation-dependent mechanism, then the plasmid is introduced into bacterial ( E. coli ) cells for expression. (hideyuki-ts.com)
The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells. (justia.com)
22) * After my arrival in the United States, I participated in a major study concentrating on structural changes of the chromosomes in the white blood cells following LSD administration. (druglibrary.net)
The first group includes papers describing structural changes of the chromosomes produced by LSD in vitro , ** in these experiments various concentrations of LSD are added to cultures of cells from human, animal, or plant tissues in a test-tube. (druglibrary.net)
Sophisticated lab facilities are needed Plants cells, tissues and organs grown in artificial medium Optimum environmental conditions are provided Aseptic condition is maintained in tissue culture laboratory Plants are multiplied by asexual method/vegetative methods Progenies obtained are true to type because of asexual reproduction 5. (pdfcoffee.com)
00:00:53.17 to biological clocks, 00:00:55.13 first talk about the genes, 00:00:57.27 and then about the clocks in cells, 00:01:00.22 and then how they're organized in the body. (ibiology.org)

The ADE2 gene is used for yeast selection with consequent disruption of the URA3 gene, allowing direct modification of YACs within the common host strain AB1380, and providing a simple test for correct targeting. (ox.ac.uk)
Cloning vectors in yeast include yeast artificial chromosomes (YACs). (fullsportpress.net)

While genetics involves examining the organism's DNA (genes) and the effect of inheritance on the genomic expression, genomics involves the collective characterization of the genes, the relationship existing between each gene, and the effects of such associations on the organism. (cannabistech.com)
The laboratory house mouse has maintained a standard 40-chromosome karyotype - or the full picture of an organism's chromosomes - after more than 100 years of artificial breeding," said co-first author LI Zhikun, researcher in the Chinese Academy of Sciences (CAS) Institute of Zoology and the State Key Laboratory of Stem Cell and Reproductive Biology. (eurekalert.org)

Housekeeping genes in the FSCN1 - ACTB (F-A) locus (Fig. 1a ) have relatively steady expression levels across various tissues and thus exogenous gene silencing resulting from chromatin inactivation might be avoided. (biomedcentral.com)

S. cerevisiae has ∼ 6,000 genes, so measuring all pairwise PPIs in one environment requires ∼ 18 million strains to be assayed, each of which characterizes a single PPI. (nature.com)

If you look at the karyotype, the chromosome map, you will see they are all different sizes. (nih.gov)
image: By fusing two medium-sized chromosomes, researchers produced the first sustainable engineered karyotype for lab mice. (eurekalert.org)
In results published today (Aug. 25) in Science , the researchers reveal that chromosome level engineering can be achieved in mammal, and they successfully derived laboratory house mouse with novel and sustainable karyotype, providing critical insight into how chromosomal rearrangements may influence evolution. (eurekalert.org)
Over longer time scales, however, karyotype changes caused by chromosome rearrangements are common. (eurekalert.org)
Using the shorter chromosomes and reducing the total number of chromosomes from 20 pairs to 19 enabled them to create a new karyotype in mice that looked healthy and normal despite having completely different chromosomes from mice in nature. (seculartalk.net)

construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in aspergillus oryzae. (liverpool.ac.uk)

Consequently, these hybrid promoters are useful for the controlled expression of foreign genes at high levels in E. coli. (wikipedia.org)
First, the rare codons in the foreign genes can be substituted with prevalent codons, but this is not always reliable. (hideyuki-ts.com)

10/21/2011 · What is the application of gene cloning in The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. (fullsportpress.net)
The plethora of mixes, which before the advent of genomics would have been formerly considered impossible, are created from the mapping out and fusion of the desired parent strain genomic sequence to identify and highlight favorable traits and create an entirely new kind. (cannabistech.com)
In certain conditions, the retroelements -molecules that possess the ability to evolve themselves and transform other genes - on the chromosome 6, mutate, duplicate and alter the structure of other genes, transforming the entire sequence. (cannabistech.com)
different online artificial receptor, longitudinal property and 0K1hisds+∫ backbone of sequence restriction of areas,4,368 data: pFlpBtM-II for expression Steroid, 2000 Mol. (scoutconnection.com)
We are no longer limited to asking questions about the differential expression of genes where we have sequence information available to make an array. (enseqlopedia.com)
This pdf left a removed sequence of 322 outcome outcomes( polynucleotide) with 64 bilirubin cDNA convergence( 80 recombination vector) to the different start gene colony sub-model construct Der f 1. (fellowshipbaptistsb.org)
Gene expression is the process by which the inheritable information in a gene , such as the DNA sequence , is made into a functional gene product , such as protein or RNA . (wikidoc.org)

View Answer Workspace Report Discuss in Forum High ethanol concentration A. promotes yeast growth B. inhibits yeast growth C. promotes bacterial growth D. inhibits bacterial growth. (vnedu.org)

Genomic sequencing analysis showed that the artificial spore-derivative hybrid is an allotriploid, which is very common among natural hybrids. (frontiersin.org)
Called genomic imprinting, this is where a dominant gene may be marked active, while a recessive gene is marked inactive. (eurekalert.org)
random to their competing genomic online artificial risk and the attP of the such trial in suppressor target pIRES2-EGFP-TSLC1 trait4 residues are again associated for the conditionsPrivacy of sequences for collection with a mRNA of elsewhere 50 polynucleotide among the polynomial differences( Figure 1). (scoutconnection.com)
Because of their role in genomic instability and gene amplification in some human cancers, it is important to develop systematic approaches to detect and characterize DNA palindromes. (biomedcentral.com)

The possibility of inducing structural changes in the chromosomes by exogenous agents such as radiation, viruses, and a variety of chemicals, has been a subject of great scientific interest for a long time. (druglibrary.net)
After a systematic selection of target sites and transgenic colonies, we successfully obtained nine exogenous natural resistance-associated macrophage protein-1 ( NRAMP1 ) gene-inserted cows through the Cas9n strategy. (biomedcentral.com)

Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation , morphogenesis and the versatility and adaptability of any organism . (wikidoc.org)
Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) the gene in the organism. (wikidoc.org)

The researchers found that the weakened fertility resulted from an abnormality in how chromosomes separated after alignment, Wang said. (eurekalert.org)

A major mutation common in leukemia is called chromosome 16 inversion. (nih.gov)
METHOD: Mutation screening of positional candidate genes was performed in two stages. (neurotransmitter.net)

295 "Gene escape model: transfer of heavy metal resis -tance genes from E.coli":App. (globalchange.com)
A. Human or rat gene for growth hormone B. Chicken gene for delta crystalline protein C. E. coli gene for β-galactosidase D. All of the above. (vnedu.org)

Since the mid-1990s, questions on whether a single gene encodes the enzymes responsible for the conversion of CBGA to THC or CBD with two variants or two closely linked genes have risen, causing scientists to delve deeper into this mystery with the science of genomics. (cannabistech.com)

A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. (lookformedical.com)

The authors recently mapped a susceptibility locus for autism to chromosome region 2q24-q33 (MIM number 606053). (neurotransmitter.net)
Here, we report the first application of single Cas9 nickase (Cas9n) to induce gene insertion at a selected locus in cattle. (biomedcentral.com)

better source needed] For example, the PTAC system is used for fusion protein expression within the PMAL-C2X expression. (wikipedia.org)
And when they have this inversion, they always have a fusion protein, CBFb-SMMHC. (nih.gov)
The fusion protein blocks normal hematopoiesis. (nih.gov)
The interesting thing is that this fusion protein binds to another protein called RUNX1. (nih.gov)
as, only 10 online artificial of all access models followed to the Protein Data Base( PDB) are suggested purified after home in several primers. (scoutconnection.com)
then, introns distributed to joint online artificial photosynthesis 2016 integrase like the Helmholtz Protein Sample Production Facility( PSPF) have a longitudinal site of blunt mortality Carriers obtaining changes, structural construct survival and the embodiment efficiency RRS exchange( BEVS). (scoutconnection.com)
the tri6 gene, encoding a zinc finger protein involved in the regulation of trichothecene biosynthesis, from Fusarium sporotrichioides (Proctor et al. (justia.com)
The F1 effects note download modern drying technology computational on the gene protein, and can change done as components from one energy to another. (naturheilpraxis-gisbert-fussek.de)
Subsequently, we demonstrate that a single Cas9n-induced single-strand break can stimulate the insertion of the natural resistance-associated macrophage protein-1 ( NRAMP1 ) gene with reduced, but still considerable, off-target effects. (biomedcentral.com)
Several steps in the gene expression process may be modulated, including the transcription step and the post-translational modification of a protein. (wikidoc.org)
Non-protein coding genes (e.g. rRNA genes, tRNA genes) are not translated into protein. (wikidoc.org)
Another commonly used method for assaying the amount of a particular protein in a cell is to fuse a copy of the protein to a reporter gene such as Green fluorescent protein , which can be directly imaged using a fluorescent microscope. (wikidoc.org)
Any step of gene expression may be modulated, from the DNA-RNA transcription step to post-translational modification of a protein. (wikidoc.org)

Other researchers have successfully engineered chromosomes in yeast but attempts to move the techniques to mammals have not been fulfilled. (eurekalert.org)
The paper was published in the academic journal Sciences On Thursday, the research team reported the first-ever modification of genes in mammals at a scale as large as chromosomes. (seculartalk.net)
For the first time in the world, we have achieved a complete rearrangement of chromosomes in mammals, and achieved a new breakthrough in synthetic biology," Lee was quoted as saying. (seculartalk.net)

We hypothesize that there might be at least three types of autism susceptibility genes/mutations that can be (i) specific to an individual patient or family, (ii) in a genetically isolated sub-population and (iii) a common factor shared amongst different populations. (neurotransmitter.net)
In the present study, genes across the 2q24-q33 interval were analyzed to identify an autism susceptibility gene in this region. (neurotransmitter.net)
We find several new and unexpected genes can impact susceptibility to fluconazole. (bvsalud.org)

RUNX1 itself is the target of several chromosome rearrangements seen in leukemia. (nih.gov)
The result may provide critical insight into how rearrangements of chromosomes - the tidy packages of organized genes, provided in equal number from each parent, which align and trade or blend traits to produce offspring - influence evolution. (eurekalert.org)

Linkage and association of the mitochondrial aspartate/glutamate carrier SLC25A12 gene with autism. (neurotransmitter.net)
Two single nucleotide polymorphisms (SNPs) that showed evidence for divergent distribution between autistic and nonautistic subjects were identified, both within SLC25A12, a gene encoding the mitochondrial aspartate/glutamate carrier (AGC1). (neurotransmitter.net)
A bZIP transcription repressor of mitochondrial function (CIN5) positively regulated Pdr1 while hundreds of genes encoding mitochondrial proteins were confirmed as negative regulators of Pdr1. (bvsalud.org)

Here especially highlighted variations of FISH are molecular combing, chromosome orientation-FISH (CO-FISH), telomere-FISH, parental origin determination FISH (POD-FISH), FISH to resolve the nuclear architecture, multicolor-FISH (mFISH) approaches, among other applied in chromoanagenesis studies, Comet-FISH, and CRISPR-mediated FISH-applications. (frontiersin.org)
Proust-Lima C, Sene M, Taylor JMG, Jacqmin-Gadda H. Joint Molecular online artificial photosynthesis aspects for physiological and time-to-event parameters: a liver. (scoutconnection.com)

IPTG), but when the foreign gene contains a large number of rare codons, this is not enough. (hideyuki-ts.com)

Without doubt, the first human transforming gene, and more broadly, oncogenes and anti-oncogenes, had been previously theorized by Theodor Boveri. (jcancer.org)

The vast majority of genes in eukaryotes are located within chromosomal structures in the nucleus of the cell. (nature.com)
We summarise the evidence for the adaptive importance of cytoplasmic genes before detailing coadaptation between the cytoplasm and the nucleus, and amongst cytoplasmic components. (nature.com)
Chromosomes, which carry DNA in the cell nucleus, naturally disintegrate and recombine via a complex and delicate process. (seculartalk.net)

Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)

A. Transfer of whole nuclei B. Transfer of whole individual chromosomes or fragment C. Transfer of DNA. (vnedu.org)

He suggested to introduce the term chromosomics being equal to cytogenomics to bring the three-dimensional morphologically of chromosomes into the focus of research, as this is essential for gene regulation. (frontiersin.org)
Regulation of gene expression is the cellular control of the amount and timing of appearance of the functional product of a gene. (wikidoc.org)

Without the three naturally imprinted regions, the researchers' engineered imprinting pattern could take hold, allowing them to fuse specific chromosomes. (eurekalert.org)

And we found that the inversion generates a fusion gene between the core binding factor beta gene, or CBFB , and MYH11 , the gene encoding smooth muscle myosin. (nih.gov)

310 "A high efficiency method for site-directed mutagen -esis with any plasmid":Gene;1989 (Dec 7);84(1);p153-7.Average yield of mutants was 60% with simple and rapid techniques. (globalchange.com)

Mice, however, clone in Much lower rate of standard experimental mice due to a defect in how chromosomes separate after alignment. (seculartalk.net)

Protoplast fusion and embryo culture techniques are used to integrate desirable genes from wild species to the cultivated species 15. (pdfcoffee.com)
Male sterile lines (cybrids) for the hybrid seed production can be developed using protoplast fusion technique. (pdfcoffee.com)

Chinese scientists have succeeded in recombining mouse chromosomes to create the world's first mammal with fully reprogrammed genes. (seculartalk.net)

Although four sources of RNA were evaluated Stratageneâ€™s Universal Human Reference RNA and Ambionâ€™s Human Brain RNA samples were chosen because of the number of genes expressed at a detectable level, and the size of the fold changes between the two samples. (enseqlopedia.com)
Amazingly, the first human cancer gene was cloned only thirty years ago. (jcancer.org)

Telomeres are chromosome-capping structures that protect the ends of eukaryotic chromosomes from degradation, end-to-end fusions and illegitimate recombination. (crcm-marseille.fr)
2022). The lab has been also pioneering in describing the role of RPA in replication, maintenance and recombination of telomeres in yeast models (Maestroni et al. (crcm-marseille.fr)

Even with recent advances such as high density yeast arrays and automated mating and scoring 11 , the time and reagent costs associated with performing screens across several environments make these studies impractical. (nature.com)

Almost every chromosome is affected by numeric or structural aberrations. (neurotransmitter.net)
Serum expressed against structural Pso online artificial photosynthesis 2016 1 time with young Der connection 1 and increased Pso 5log1+t+bi1t+bi0+ε 1 to transfer estimated to the replacement of P. Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, Scotland. (scoutconnection.com)

The tac promoter is used to control and increase the expression levels of a target gene and is used in the over-expression of recombinant proteins. (wikipedia.org)

The team uses yeast models to study the mechanisms that maintain telomere length, telomere replication and cellular responses to telomere erosion. (crcm-marseille.fr)

Nearly everyone whoâ€™s worked on differential gene expression in the last fifteen years has heard of the MAQC (MicroArray Quality Control) study. (enseqlopedia.com)
This study requires a combination of chromosome painting and fluorescent in situ hybridization (FISH) with yeast artificial chromosomes (YAC) and cosmids. (medscape.com)

In contrast to previously described BRAF fusions, MTAP-BRAF and MS4A6A-BRAF do not respond to the paradox breaker RAF inhibitor (RAFi) PLX8394 due to stable fusion dimerization mediated by the N-terminal fusion partners. (authorea.com)

By varying the IPTG concentration the amount of gene product cloned downstream from PTAC can be varied over several orders of magnitude. (wikipedia.org)

Gene Cloning and Its Medical Uses Qasim Munye 18th September 2014 Respond In its simplest form, for example in the treatment of single gene (monogenic) disorders, gene therapy can be considered the replacement of a defective gene in an individual with a functional "wild type" version of the gene to reverse the pathology. (fullsportpress.net)
At an individual level, fusions or translocations can lead to missing or extra chromosomes or even to such diseases as childhood leukemia. (eurekalert.org)

The N-terminal A domain differs among the four subtypes of Ca 2+ channel β subunits (β 1 -β 4 ) primarily as the result of two duplications of an ancestral gene containing multiple alternatively spliced exons. (jneurosci.org)

Gene cloning involves separation of specific gene or DNA fragments from a donor cell, attaching it to small carrier molecule called vector and then replicating this recombinant vector into a host cell. (fullsportpress.net)

The tac promoter is, therefore, inducible by IPTG (Isopropyl β-D-1-thiogalactopyranoside), whilst also allowing higher maximum gene expression than either the lac or trp promoters. (wikipedia.org)

The process is used to generate quantities of DNA molecule segments or copies of specific genes. (fullsportpress.net)

The clinical C31 online artificial coverage treatments enriched for time Integrate have aimed from the Eukaryotic transgenic vertebrate scan Purification accuracy HEK293 and CHO microRNAs, which are from artifacts of the observed Hamster. (scoutconnection.com)
A. Variation is caused by genes B. Variation is caused by the environment C. Variation can be caused by both genes and the environment D. None of the above Which of the following gene have been introduced into the transgenic fish? (vnedu.org)
Thus, this repair pathway causes a major safety problem for gene targeting strategies, particularly for gene therapy and transgenic animal production [ 15 , 16 ]. (biomedcentral.com)
Consequently, we determined the feasibility of using Cas9n-induced SSB to stimulate homology-directed repair (HDR) and therefore provided a safe alternative for gene insertion and transgenic animal generation. (biomedcentral.com)

examination of their progeny gave rise to the prior mentioned cannabinoids been controlled by a single gene. (cannabistech.com)

Method of gene cloning is useful in studying the structure and function of genes in detail. (fullsportpress.net)
6/3/2017 · Gene cloning- Steps involved in gene cloning Gene cloning. (fullsportpress.net)

Previous studies suggest the transcription factor Pdr1 and several target genes encoding ABC transporters are critical elements of pleiotropic defense against azoles and other antifungals. (bvsalud.org)

The karyotypes with chromosome 2 fused to the top of chromosome 1 did not lead to any full-term mouse pups, while the opposite arrangement produced pups that grew into larger, more anxious, and physically slower adults, compared to the mice with fused 4 and 5 chromosomes. (eurekalert.org)
Only the mice with fused 4 and 5 chromosomes were able to produce offspring with wild-type mice, but at a much lower rate than standard lab mice. (eurekalert.org)
When Lee and his colleagues manipulated chromosomes by stitching together two very long pieces of the chromosome, they also made some mistakes that either resulted in the death of the producing mice, distorted samples, or strange behaviors. (seculartalk.net)
The reprogrammed mice were able to mate and procreate with normal mice, suggesting that artificial changes can be passed down through generations. (seculartalk.net)

We recently identified rare heterozygous variations in RPA genes in patients exhibiting short telomeres and idiopathic pulmonary fibrosis (Sharma et al. (crcm-marseille.fr)

The expression of many genes is regulated after transcription (i.e., by microRNAs or ubiquitin ligases ), so an increase in mRNA concentration need not always increase expression. (wikidoc.org)

So what we designed a fluorescence in situ hybridization procedure to detect the fusion gene. (nih.gov)

HR is inefficient in many cell types and thus a site-specific double-strand break (DSB) is often introduced by site-specific homing endonucleases or artificial endonucleases, such as zinc finger and TALE nucleases. (biomedcentral.com)

Anatomy58

Organisms17

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Chemicals and Drugs41

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Psychiatry and Psychology1

Phenomena and Processes151

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Technology, Industry, Agriculture2

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Genome9

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Cells13

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Foreign genes2

Sequence7

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