• 20 ] proposed auto covariance (AC) to extract information from protein sequences and used support vector machine model to predict PPIs in the Saccharomyces cerevisiae dataset with 88.09% accuracy. (biomedcentral.com)
  • 21 ] proposed local descriptors (LD) to represent protein sequences and successfully predicted potential PPIs on Saccharomyces cerevisiae (core subset) dataset by implementing K-neighbor model. (biomedcentral.com)
  • The best-studied model of eukaryotic cells is the yeast Saccharomyces cerevisiae . (cas.cz)
  • These results strongly indicate that the outer chain processing of N-glycans by H. polymorpha significantly differs from that by Saccharomyces cerevisiae, thus generating much shorter mannose outer chains devoid of terminal alpha 1,3-linked mannoses. (unboundmedicine.com)
  • The best source of chromium is brewer's yeast. (medlineplus.gov)
  • However, many people do not use brewer's yeast because it causes bloating ( abdominal distention ) and nausea . (medlineplus.gov)
  • A phylogenetic analysis of 27 plant species clustered the 14-3-3 proteins into four groups. (wikipedia.org)
  • To gain fundamental mechanistic insights into this process, a side-by-side quantitative comparison of CME was performed on two distantly related yeast species. (biorxiv.org)
  • These studies identified conserved CME mechanisms and species-specific adaptations and have broad implications that extend from yeast to humans. (biorxiv.org)
  • In more distant species, putative homologues were detected for Ykl155c, which shares conserved motifs with uncharacterized proteins of higher eukaryotes including humans. (pasteur.fr)
  • In bovine brain samples, 14-3-3 proteins were located in the 14th fraction eluting from a DEAE-cellulose column and in position 3.3 on a starch electrophoresis gel. (wikipedia.org)
  • The starchy endosperm thereby is characterized as a storage site, accumulating starch and seed storage proteins (SSPs) 2 . (nature.com)
  • 14-3-3 proteins are a family of conserved regulatory molecules that are expressed in all eukaryotic cells. (wikipedia.org)
  • Over more than 16 years, CUSABIO has established five systems from prokaryotic to eukaryotic to express the recombinant protein. (cusabio.com)
  • Thus, endomembrane-modifying proteins within the endomembrane system will have an influence on the final grain quality/yield and recombinant protein production. (nature.com)
  • This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)
  • Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • Eukaryotes can tolerate the loss of a single 14-3-3 gene if multiple genes are expressed, but deletion of all 14-3-3s (as experimentally determined in yeast) results in death. (wikipedia.org)
  • The presence of large gene families of 14-3-3 proteins in the Viridiplantae kingdom reflects their essential role in plant physiology. (wikipedia.org)
  • Here we report the temporal measurements of absolute RNA and protein levels per gene within a mixed bacterial-archaeal consortium. (nature.com)
  • Beclin l, the mammalian counterpart of the yeast Atg6 gene, is an essential protein in autophagy ( 10 ). (spandidos-publications.com)
  • Liu et al ( 13 ) reported that Beclin 1 gene deletion by either RNA interference or the autophagy inhibitor 3-methyladenine (MA) significantly enhances melatonin-induced apoptosis in mouse hepatoma H22 cells. (spandidos-publications.com)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
  • however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
  • [ 7 ] C albicans prevacuolar protein sorting gene VPS4 is required for extracellular secretion of the secreted aspartyl proteases Sap2p and Saps4-6p. (medscape.com)
  • Protein-protein interactions (PPIs) dominate intracellular molecules to perform a series of tasks such as transcriptional regulation, information transduction, and drug signalling. (biomedcentral.com)
  • In both yeasts, Arp2/3 complex activation drives membrane invagination when triggered by the accumulation of ∼70 WASP molecules. (biorxiv.org)
  • While the field of microbiology has adapted to the study of complex microbiomes via modern meta-omics techniques, we have not updated our basic knowledge regarding the quantitative levels of DNA, RNA and protein molecules within a microbial cell, which ultimately control cellular function. (nature.com)
  • The invention relates to a protein high-calcium steamed bun and a preparation method thereof. (google.com)
  • in addition, the protein high-calcium steamed bun also has the functions on calming the nerves, beautifying the features, lowering the cholesterol, removing the heart-fire, eliminating the dryness, resisting the tumor, clearing away te heat and toxic materials, nourishing the Yin and replenishing the kidney and is suitable for be eaten by all people for a long time. (google.com)
  • The invention belongs to food processing technology field, relate to a kind of steamed bun, relate in particular to a kind of protein high-calcium steamed bun and preparation method thereof. (google.com)
  • The object of this invention is to provide a kind of protein high-calcium steamed bun and preparation method thereof, the present invention has soft good to eat, nutritious feature. (google.com)
  • Live-cell super resolution analysis of endocytosis was also performed in fission yeast (Arasada, 2018). (biorxiv.org)
  • The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
  • Yarrah Organic Grain Free with Organic Chicken & Fish is made with selected organic ingredients , including delicious organic chicken as a source of easily-digestible protein. (zooplus.co.uk)
  • 14-3-3 proteins directly regulate CaMKKalpha when the enzyme is phosphorylated by protein kinase A on either Ser74 or Ser475. (cusabio.com)
  • Cereal Mash/Enzyme Rest/3.5-pounds grits, 14-ounces 6-row/1-TBSP Amylase. (brewersfriend.com)
  • Started the cereal mash with 3-gallons of water, 3.5-pounds of yellow corn grits, 14-ounces 6-row barley, and 1-TBSP of amylase enzyme. (brewersfriend.com)
  • Predicting tissue-specific mRNA and protein abundance in maize: A machine learning approach. (usda.gov)
  • Blocking the interaction between PIF7 and 14-3-3 proteins or reducing the expression of 14-3-3 proteins accelerates shade-induced nuclear localization and de-phosphorylation of PIF7, and enhances the shade phenotype. (elifesciences.org)
  • These proteins contain a number of known common modification domains, including regions for divalent cation interaction, phosphorylation & acetylation, and proteolytic cleavage, among others established and predicted. (wikipedia.org)
  • It can also correctly predict the protein interaction of cell and tumor information contained in one-core network and crossover network.The SDNN-PPI proposed in this paper not only explores the mechanism of protein-protein interaction, but also provides new ideas for drug design and disease prevention. (biomedcentral.com)
  • 14 -3-3 proteins and spinocerebellar ataxia type 1: from molecular interaction to human neuropathology. (nih.gov)
  • Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) modulates cellular apoptosis, which is involved in the occurrence and development of liver cancer. (spandidos-publications.com)
  • Furthermore, it was confirmed that Beclin 1 expression by NS5A was involved in the negative regulation of starvation‑induced liver cancer apoptosis, which was accompanied by reduced p53 and apoptosis regulator Bax expression, as well as decreased caspase‑3/-7 activation. (spandidos-publications.com)
  • GO enrichment analysis of the interacting proteins identified terms related to apoptosis, protein binding, locomotion, hippo signalling and leukocyte and lymphocyte differentiation, supporting the experimental findings. (biomedcentral.com)
  • Another newly identified ribosomal protein, Ygl129c, was previously shown to be a member of the DAP-3 family of mitochondrial apoptosis mediators. (pasteur.fr)
  • Raf-1 Bad - see Bcl-2 Bax Cdc25 Akt SOS1 - see RSK YWHAB - "14-3-3 beta" YWHAE - "14-3-3 epsilon" YWHAG - "14-3-3 gamma" YWHAH - "14-3-3 eta" YWHAQ - "14-3-3 tau" YWHAZ - "14-3-3 zeta" SFN or YWHAS - "14-3-3 sigma" (Stratifin) The 14-3-3 proteins alpha and delta (YWHAA and YWHAD) are phosphorylated forms of YWHAB and YWHAZ, respectively. (wikipedia.org)
  • Naturally contains a high amount of omega 3 & 6 fatty acids, which support the development and function of the central nervous system (including the brain) & benefit hair coat health. (karoutexpress.com)
  • This hypoallergenic protein is a great source of energy for your dog and is rich in omega-3 and -6 fatty acids, which soothe inflamed skin and encourage new hairs to grow. (medicanimal.com)
  • While the better known omega-3 fatty acids from fish oil, EPA and DHA, had negative impacts on LDL 'bad' cholesterol levels, but did lead to beneficial effects to levels of a protein that affects blood clotting. (bakeryandsnacks.com)
  • "This suggests that these n-3 fatty acids derived from different sources might have their own specific effects on cardiovascular risk markers," ​ wrote the scientists in the European Journal of Clinical Nutrition ​ (Vol. 60, pp. 978-984). (bakeryandsnacks.com)
  • Our findings indicate that n-3 fatty acids from both plant and marine sources do not affect the lipid profile equally favorable in elderly subjects as oleic acid. (bakeryandsnacks.com)
  • These nutrients include a rich source of Omega-3 fatty acids EPA & DHA, as well as borage oil. (hyperdrug.co.uk)
  • This gentle recipe excludes grains and cereals and includes fresh ocean white fish as an easily digestible source of protein which is naturally high in omega-3 essential fatty acids. (ardengrange.com)
  • These studies demonstrate a phosphorylation-dependent translocation of PIF7 when plants are in shade and a novel mechanism involving 14-3-3 proteins, mediated by the retention of PIF7 in the cytoplasm that suppresses the shade response. (elifesciences.org)
  • Binding of 14-3-3 has little effect on Cdc25C activity, and it is believed that 14-3-3 regulates Cdc25C by sequestering it to the cytoplasm, thereby preventing the interactions with CycB-Cdk1 that are localized to the nucleus at the G2/M transition. (wikipedia.org)
  • 14-3-3 proteins bind to and sequester the transcriptional coregulators YAP/TAZ to the cytoplasm, inhibiting their function. (wikipedia.org)
  • 14-3-3 proteins activate the auto-inhibited plasma membrane P-type H+ ATPases. (wikipedia.org)
  • To cope with this, yeast cells possess more than ten different transporters for K + , Na + , and H + localized in the plasma membrane (ensuring import and export) or in membranes of intracellular organelles (transporting cations between cytosol and cell organelles). (cas.cz)
  • Both budding and fission yeast have high internal turgor pressure, which strongly opposes plasma membrane invagination necessary for endocytosis. (biorxiv.org)
  • Seven genes encode seven distinct 14-3-3 proteins in most mammals (See Human genes below) and 13-15 genes in many higher plants, though typically in fungi they are present only in pairs. (wikipedia.org)
  • Our laboratory possesses a large collection of yeast strains with deletions of genes for a single transport system or with combinations of these deletions. (cas.cz)
  • RT "Identification of Barkor as a mammalian autophagy-specific factor for RT Beclin 1 and class III phosphatidylinositol 3-kinase. (pdbj.org)
  • Studied proteins are tagged with fluorescent markers and cells producing these proteins are visualized by fluorescent microscope. (cas.cz)
  • vitamins, provitamins and chemically well defined substances of analogous effect: Vitamin A20000U.I., Vitamin D32000U.I., Vitamin E (α-tocopherol) 115 mg, Vitamin C 100 mg.Oligoelements: iron 150 mg (as ferrous carbonate), iodine 3 mg (as potassium iodide), copper 10 mg (as cupric sulfate-hydrate), manganese 49 mg (as manganous oxide), zinc 165 mg (as zinc oxide), selenium 0.34 mg (as selenitosódico). (huellacanina.com)
  • TG(12:0/14:0/18:1(9Z)) belongs to the family of triradyglycerols, which are glycerolipids lipids containing a common glycerol backbone to which at least one fatty acyl group is esterified. (ymdb.ca)
  • 1975). "Lipids of yeasts. (ymdb.ca)
  • There are common recognition motifs for 14-3-3 proteins that contain a phosphorylated serine or threonine residue, although binding to non-phosphorylated ligands has also been reported. (wikipedia.org)
  • The intracellular concentration of potassium and sodium cations, as well as of protons (pH), is strictly regulated via the activity of a series of membrane proteins that mediate the flux of cations and protons with various transport mechanisms. (cas.cz)
  • These data suggest that increases in 14-3-3 proteins mediate THC-induced neuroprotection under conditions of NMDA-induced cellular stress. (cusabio.com)
  • By using label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. (nature.com)
  • In order to perform studies of such design and test if previously defined quantitative data about the functioning of microorganisms (i.e., protein-to-RNA ratio) is applicable to real world consortia, we must first sample microbial communities across transition events and employ quantification techniques that are absolute. (nature.com)
  • Our analysis of this data reveals an absolute protein-to-RNA ratio of 10 2 -10 4 for bacterial populations and 10 3 -10 5 for an archaeon, which is more comparable to Eukaryotic representatives' humans and yeast. (nature.com)
  • 22 ] utilized four categories of protein sequence information (AC, CT, LD, MAC) to encode proteins as feature vectors focusing on dimensionality reduction and proposed a new hierarchical PCA-EELM (principal component analysis-integrated extreme learning machine) model to predict protein interactions. (biomedcentral.com)
  • Thus the formation of aggregates renders these toxic proteins resistant to proteasomal degradation and initiates the accumulation of polyGln proteins and polyGln-interacting proteins. (5dok.org)
  • Botulism is caused by botulinum neurotoxins (BoNTs), extremely toxic proteins which can induce respiratory failure leading to long-term intensive care or death. (cdc.gov)
  • We own protein expression technology based on a series of protein expression and purification technology, including protein expression technology shown by Baculovirus outer membrane proteins (OMP), and membrane protein expression technology, etc. (cusabio.com)
  • Western blot analysis showed that rFg14-3-3e protein is recognized by sera from goats experimentally infected with F. gigantica and immunofluorescence staining using rat anti-rFg14-3-3e antibodies demonstrated the specific binding of rFg14-3-3e protein to the surface of goat PBMCs. (biomedcentral.com)
  • Nevertheless, in neurodegenerative diseases these proteins accumulate with disastrous consequences for neurons, eventually leading to cell death. (5dok.org)
  • citation needed] 14-3-3 proteins are structurally similar to the Tetratrico Peptide Repeat (TPR) superfamily, which generally have 9 or 10 alpha helices, and usually form homo- and/or hetero-dimer interactions along their amino-termini helices. (wikipedia.org)
  • Genetics and live-cell imaging revealed core CME spatiodynamic similarities between the two yeasts, though two-zone actin assembly is a fission yeast-specific mechanism, which is not essential for CME. (biorxiv.org)
  • For biological systems, Q peaks were first observed in protein from different perspectives [3,7,9,13,15,21-23] , the detailed molec- powders and attributed to 1H-14N spin pairs [9], which were also ular mechanism of the ubiquitous 1H-14N Q peaks in biological sys- thought to be of critical importance for the background 1H relaxa- tems has not been established. (lu.se)
  • abstract = "Myo2p is an essential class V myosin in budding yeast with several identified functions in organelle trafficking and spindle orientation. (elsevierpure.com)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
  • Calories 230, Total Fat 11g (14% DV), Sat. Fat 6g (30% DV), Trans Fat 0g, Cholest. (fastachi.com)
  • Together our data identified promising targets to be genetically engineered to modulate seed storage protein accumulation that have a growing role in health and nutritional issues. (nature.com)
  • Cytoskeletal proteins as targets for organophosphorous compound and aliphatic hexacarbo n- induced neurotoxicity. (cdc.gov)
  • Autophagy was blocked by inhibitors 3‑methyladenine and chloroquine, or via knockdown of Beclin 1. (spandidos-publications.com)
  • RT "Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate RT autophagy at different stages. (pdbj.org)
  • The basic steps of PPIs prediction based on protein sequence consist of two parts: protein coding method and machine learning model. (biomedcentral.com)
  • Sequence analysis revealed that rFg14-3-3e protein had 100% identity to 14-3-3 protein from Fasciola hepatica . (biomedcentral.com)
  • Yet, genetic and biochemical studies identified only 14 proteins, half of which were predictable by sequence homology with prokaryotic ribosomal components of the small subunit. (pasteur.fr)
  • The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
  • The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)
  • PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
  • Based on proteome annotation and a homology search, 94 proteins associated with the endomembrane system were identified that exhibited significant changes in abundance during grain development. (nature.com)
  • notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified PBs at the early development stages. (nature.com)
  • Though endocytic protein abundance in S. pombe and S. cerevisiae are more similar than previously thought, membrane invagination speed and depth are two-fold greater in fission yeast than in budding yeast. (biorxiv.org)
  • Mix the flour, salt, and yeast with a wooden spoon or dough whisk in a large bowl. (dishcrawl.com)
  • In addition, NS5A significantly increases the expression of inducible nitric oxide synthase, cyclin D1 and nuclear factor-κB, but decreases p53 protein expression in HepG2 cells ( 6 ). (spandidos-publications.com)
  • Using the bicoid 3' UTR, localized Toll(10b) products result in the formation of an ectopic, anteroposterior (AP) Dl nuclear gradient along the length of the embryo. (sdbonline.org)
  • NS5A upregulates Beclin 1 mRNA and protein expression in a HCV NS5A-transactivated protein 9 (NS5ATP9)-dependent manner ( 15 ). (spandidos-publications.com)
  • Previously, our laboratory reported in several publications on the development of the Endopep-MS method as an assay for BoNT detection and serotype differentiation [ 3 , 4 ]. (cdc.gov)
  • For example, when Escherichia coli is grown axenically in steady state, we can expect that each RNA molecule corresponds to 10 2 -10 4 of the matching protein (absolute protein-to-RNA ratio, hereafter referred protein-to-RNA ratio) and the variation in the level of cellular RNA explains ~29% of the variation in the amount of detectable protein 2 . (nature.com)
  • Our data suggest that rFg14-3-3e protein can influence various cellular and immunological functions of goat PBMCs in vitro and may be involved in mediating F. gigantica pathogenesis. (biomedcentral.com)
  • Cereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (PBs) and protein storage vacuoles (PSVs), for the accumulation of storage proteins. (nature.com)
  • RT "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p- RT Vps15p protein sorting system. (pdbj.org)
  • RT "A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, RT VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative RT endocytic traffic. (pdbj.org)