• Furthermore, Xenopus oocytes are a leading system for studies of ion transport and channel physiology. (wikipedia.org)
  • The RNAs for the separate subunits were transcribed in vitro from cDNAs inserted in pSP64T vectors and microinjected in Xenopus oocytes. (pnas.org)
  • Xenopus oocytes assembled functional AcChoRs from the subunit-specific RNAs. (pnas.org)
  • These results suggest that transcript availability may control receptor expression in Xenopus oocytes. (pnas.org)
  • Here, we describe techniques for purifying the MeCP2-contining histone deacetylase complex from Xenopus laevis oocytes. (springer.com)
  • All three influenza virus polymerase (P) proteins were expressed in Xenopus oocytes from microinjected in vitro transcribed mRNA analogs, with yields of up to 100 ng per oocyte. (nih.gov)
  • To confirm that the oocyte cDNA encodes a gap junction channel, the protein was over expressed in Xenopus oocytes by injection of RNA synthesized in vitro. (sciencemag.org)
  • Xenopus oocytes are naturally arrested at G2 of meiosis I. Exposure to either insulin/IGF-1 or the steroid hormone progesterone breaks this arrest and induces resumption of the two meiotic division cycles and maturation of the oocyte into a mature, fertilizable egg. (genome.jp)
  • In addition to being used to generate cell-free extracts, Xenopus oocytes and eggs can also be studying directly. (openwetware.org)
  • We have examined the initiation of polarized membrane traffic in Xenopus and show that membrane traffic is not polarized in oocytes but polarized membrane domains appear at first cleavage. (rupress.org)
  • 1995). We have carded out studies to determine whether the DNA-PK activity detected in Xenopus is associated with a Ku-like protein, and to evaluate preliminarily whether the enzyme activity varies in different stages of oocytes. (thefreelibrary.com)
  • Female African clawed frogs (Xenopus laevis) were purchased from Nasco (Wisconsin), and the oocytes were staged according to Dumont (1972). (thefreelibrary.com)
  • Electrophysiological studies that employ Xenopus oocytes include: 1) analysis of channel permeability properties and pharmacological sensitivity, 2) investigation of the properties of mutant channel subunits to address structure-function issues, 3) analysis of channel post-translational modifications, assembly and stoichiometry, 4) study of channel modulation by receptors and second messengers, 5) functional screens for genes encoding ion channels and transporters (expression-cloning). (wormbook.org)
  • The most common approach for expression of exogenous proteins in Xenopus oocytes is to inject in vitro transcribed complementary RNA into the oocyte cytoplasm ( Figure 1a ). (wormbook.org)
  • (a) Cartoon representing the process of expressing exogenous proteins in Xenopus oocytes. (wormbook.org)
  • The Xenopus serves as a model organism for the study of embryonic development, developmental biology, cell biology, and oncogenesis. (horizondiscovery.com)
  • Most importantly, an ORFeome set allows high-throughput in vivo functional-genomic screening of frog gnees in manner previously not feasible, This will help resesarchers take advantage of the strengths of Xenopus as a biomedical model organism. (xenbase.org)
  • In this regard, recent advances in adapting the transgenesis approach to Xenopus have allowed for in vivo studies of the impact of loss and gain of function of specific genes at the level of the whole organism, further enhancing the potential uses of Xenopus as an important biomedical model system. (mdpi.com)
  • Based on morphological, biochemical and karyological characters, the genus Xenopus can be divided into two main groups (subgenera), Silurana and Xenopus , and the latter into five subgroups. (thebhs.org)
  • Scientists soon realized that Xenopus was also a valuable and versatile laboratory model for basic biology, enabling studies that shed light on both fundamental mechanisms of vertebrate embryonic development and mechanisms underlying human diseases. (eurekalert.org)
  • The transcription factor Snai2, which is induced by canonical Wnt signaling to be expressed in the early CNC, is pivotal for CNC induction and migration in Xenopus . (nature.com)
  • Wade, P. A., Jones, P. L., Vermaak, D., and Wolffe, A. P. (1998) A multiple subunit Mi-2 histone deacetylase from Xenopus laevis cofractionates with an associated Snf2 superfamily ATPase. (springer.com)
  • We first demonstrate that Xenopus laevis Rpd3 (a histone deacetylase) and Sin3 (a corepressor associated to Rpd3) are expressed in premetamorphic and metamorphic tadpole tissues, suggesting their involvement in these postembryonic processes. (spandidos-publications.com)
  • The Xenopus community is in full support of this project and the ORFeome was voted as a top priority of needed Xenopus resources. (xenbase.org)
  • Clone isolation and validation of the 40% of genes not currently represented in the Xenopus ORFeome v1.0 in Gateway entry vectors. (xenbase.org)
  • The Xenopus ORFeome sequences have been cloned from Xenopus cDNA libraries or have been chemically synthesized. (xenbase.org)
  • Finally, despite the biological and physical similarities between Xenopus TCGF and mammalian IL-2, anti-human IL-2 monoclonal antibodies do not recognize Xenopus TCGF. (hindawi.com)
  • Our animal resource facility include four newly renovated rooms in the vivarium that are designated for maintaining experimentally manipulated amphibians and for maintaining and breeding stocks of outbred, partially inbred, and cloned lines of Xenopus. (rochester.edu)
  • A good starting point would be 2 gram/litre, but Xenopus are surprisingly salt-tolerant amphibians, and you could raise this a bit after a week or so to perhaps 3-5 gram/litre for a few days if needs be. (wetwebmedia.com)
  • A Xenopus model of alkaptonuria. (genetics-gsa.org)
  • The recent development of transgenic Xenopus technology offers the promise that this animal model will be utilized more than ever before. (indigo.ca)
  • This comparative model, in Xenopus , may provide an alternative to the mouse in exploring, within an evolutionary context, the interactions between tumors and their host. (pubmedcentralcanada.ca)
  • Pigment organelles in Xenopus laevis melanophores are used by the animal to change skin color, and they provide a good model for studying intracellular organelle transport. (diva-portal.org)
  • This review highlights some of the major uses and applications of the Xenopus model. (mdpi.com)
  • In addition, Xenopus is emerging as an excellent model system for toxicology, neurobiology, regenerative biology, and other areas of biomedical science [ 3 ]. (mdpi.com)
  • This development of novel research tools has proved critical to further empowering Xenopus as a model system and keeping it at the forefront of biomedical research. (mdpi.com)
  • Xenopus is remarkable for modeling human diseases including birth defects, cancer, and stem cell biology. (cshl.edu)
  • Indeed, the use of Xenopus in various areas of biomedical research including tumor biology, transplantation biology, self-tolerance, and auto immunity is steadily increasing and has significantly contributed to advances in these fields. (mdpi.com)