When active, IRE1α catalyzes the processing of a 26-nucleotide fragment (containing a Pst I restriction enzyme site) from Xbp-1 mRNA. (medscape.com)
Fig. 1 D ). NO mediates IRE1α activation, as Xbp-1 processing is attenuated by NMMA. (medscape.com)
The supernatants were removed for nitrite determination ( A ), and CHOP expression was examined by Western blot analysis of the cell lysate ( B ). After incubating for 12 or 24 h with IL-1, NMMA, and tunicamycin, rat islets (150 islets/400 μl cCMRL) were isolated and eIF2α phosphorylation and total eIF2α levels determined by Western blot analysis ( C ). IRE1α activity was assayed by examining Xbp1 splicing. (medscape.com)
We further confirmed that both mRNA and protein levels of Ire1α and spliced XBP-1 were significantly increased in βKO islets. (diabetesjournals.org)
This was recently shown for your anti apoptotic protein BI 1 that actually interacts with IRE1 and suppressed XBP and UPR 1 signaling under moderate stress conditions. (plapathway.com)
The other effect was found for Bax/Bak functioning at the ER membrane to directly interact with IRE1 and stimulate the XBP 1 division of UPR signaling. (plapathway.com)
CONCLUSION@#Estrogen can inhibit the differentiation of murine macrophages into a pro-inflammatory phenotype by up-regulating the IRE1α-XBP-1 signaling axis, thereby producing an inhibitory effect on inflammatory response. (bvsalud.org)
MRNA3
Treatment of human islets for 3 h with the NO donor compound DEA-NO results in a three- to sixfold increase in the accumulation of the UPR-responsive mRNAs encoding CHOP, ATF-4, and gadd34 (Fig. 1 E ). In addition, DEA-NO also stimulates an ∼sixfold increase in the accumulation of hsp 70 mRNA in human islets. (medscape.com)
This phenomenon is connected with the loss of PERK phosphorylation and XBP-1 mRNA splicing. (hindawi.com)
The mRNA expressions of the inflammatory factors TNF-α, IL-6, and IL-1β were detected using qRT-PCR. (bvsalud.org)
Transcription factor4
2. Plasma cell-specific transcription factor XBP-1s binds to and transactivates the Epstein-Barr virus BZLF1 promoter. (nih.gov)
Protein expression of the adaptive liver response protein STC2 and the transcription factor X-box binding protein 1 spliced (XBP-1s) were significantly elevated among NASH samples, whereas other downstream ER stress proteins including CHOP, ATF4, and phosphorylated JNK and eIF2α were not significantly changed in disease progression. (nih.gov)
Compared with COPD and donor lungs, protein levels of ER stress mediators, such as processed p50 activating transcription factor (ATF)-6 and ATF-4 and the apoptosis-inductor CHOP (C/EBP-homologous protein), as well as transcript levels of spliced X-box binding protein (XBP)-1, were significantly elevated in lung homogenates and type II alveolar epithelial cells (AECIIs) of IPF lungs. (nih.gov)
Up-regulation of the chaperone BiP/GRP78 (Binding Immunoglobulin Protein/Glucose-Regulated Protein 78) and of the transcription factor CHOP/GADD153 (C/EBP Homologous Protein/Growth Arrest and DNA Damage inducible gene 153), phosphorylation of PERK (PKR-like ER Kinase) and eIF2α (Eukaryotic Initiation Factor 2α) and splicing of XBP1 (X-box binding protein 1) was observed. (oncotarget.com)
Xbp12
The N-linked glycosylation inhibitor tunicamycin, which stimulates CHOP expression, eIF2α phosphorylation, and Xbp1 splicing (Fig. 1 B - D ), was used as a positive control for UPR activation in these experiments. (medscape.com)
Tat-haFGF 14-154 antagonized Aβ 1-42 -induced cell death and structural damage in rat primary neurons in an ADAM10-dependent manner, which, in turn, was promoted by the activation of XBP1 splicing and modulated by the PI3K-CREB pathway. (nih.gov)
Induces4
IL-1 induces UPR activation in β-cells in an NO-dependent manner. (medscape.com)
3. X-box binding protein 1 induces the expression of the lytic cycle transactivator of Kaposi's sarcoma-associated herpesvirus but not Epstein-Barr virus in co-infected primary effusion lymphoma. (nih.gov)
In older and obese diabetic patients①, hyperinsulinemia② causes cellular stress and induces the XBP-1-mediated overexpression of GRP78③ in adipose tissue, which promotes localization of GRP78 to the circulation④ and cell surface⑤, not only to endoplasmic reticulum (ER)⑥. (scitechdaily.com)
It is well established that burn injury induces hepatic acute-phase response and decreases albumin production and that these conditions persist over a prolonged period of time ( 1 - 5 ). (biomedcentral.com)
Significantly1
Consistently, AR and UPR gene expression were correlated in human PCa, and spliced XBP-1 expression was significantly upregulated in cancer compared with normal prostate. (nih.gov)
Downstream1
It is possible ER remodeling by ER found Bak together with that ER expansion downstream of XBP 1, may bring about effects on the degree of intracellular Ca2 release and therefore lead to the cellular crosstalk that defines the switch from ER stress variation to cellular collapse. (plapathway.com)
Phosphorylation2
Consistent with the time-dependent production of NO, IL-1 stimulates CHOP expression (Fig. 1 B ) in RINm5F cells and eIF2α phosphorylation in rat islets (Fig. 1 C ) that is first apparent after a 12-h incubation. (medscape.com)
Importantly, the NOS inhibitor NMMA, which prevents IL-1-induced NO production, also attenuates CHOP expression and eIF2α phosphorylation in response to IL-1. (medscape.com)
CHOP1
Additionally, exposure of Neuro-2a cells to iAs triggered endoplasmic reticulum (ER) stress identified through several key molecules (GRP 78, CHOP, XBP-1, and caspase-12), which was prevented by NAC. (ntu.edu.tw)
Inflammatory2
Their findings were further supported by the identification of heightened inflammatory response in those restrained rats where part of the activated inflammatory pathways demonstrated the upregulated expression of key mediators like c-Jun kinase (JNK) and Xbox Binding Protein-1 (XBP-1). (nature.com)
XBP 1 deletion in intestinal epithelial cells triggered natural enteritis and enhanced susceptibility to induced colitis, and an association of XBP 1 variants with both kinds of human inflammatory bowel infection was determined. (plapathway.com)
20221
As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. (oncotarget.com)
Interacts1
That end of p85 interacts with a protein known as XBP-1, helping XBP-1 journey to the cell nucleus, where it activates genes that promote a cell response known as the endoplasmic reticulum (ER) stress response. (joslin.org)
Islets1
Total RNA was isolated from rat islets (150 islets/400 μl cCMRL) treated for 24 h with the indicated concentrations of IL-1, NMMA, or tunicamycin (Tuni). (medscape.com)
Proteins1
BI 1 along with Bcl2 related proteins at the ER membrane are thereby determining the amplitude of UPR reactions. (plapathway.com)
Binding Protein2
Estrogen receptor alpha (ERα), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor (mainly found as a chromatin-binding protein) that is activated by the sex hormone estrogen. (wikipedia.org)
1. X-box-binding protein 1 activates lytic Epstein-Barr virus gene expression in combination with protein kinase D. (nih.gov)
Anti-GRP78 and anti-ATF6 antibodies Olaparib kinase inhibitor had been bought from Abcam (Cambridge, MA, USA), while anti-XBP-1s, anti-eIF2, and anti-p-eIF2 antibodies had been bought from Cell Signaling Technology (Danvers, MA, USA). (bassresearch.com)
Diffuse1
The comparison of EIT and TRACE images shows that the diffuse cloud appearance of the XBP in EIT images can be resolved as a loop structure in TRACE images (also noted by Frank & Slater 2002AAS. (nasa.gov)
Stress5
In response to mild hyperthermic stress, the Heat Shock Response (HSR) and the endoplasmic reticulum (ER) stress response pathways are activated and preserve the integrity of important signaling networks to sustain cell survival under stressful conditions [ 1 - 3 ]. (oncotarget.com)
To inhibit ER stress and XBP-1 splicing, BMDMs were pretreated either with 50 g/mL TUDCA or 5 M 48c (18) for 1 h. (bassresearch.com)
Vitamin D Promotes Protein Homeostasis and Longevity via the Stress Response Pathway Genes Skn-1, Ire-1, and Xbp-1. (zotarellifilhoscientificworks.com)
Third, WWTR1( TAZ) and YAP1 enzyme ZO-1 and 2 cells( Remue et al. (evakoch.com)
Estrogen2
In humans, ERα is encoded by the gene ESR1 (EStrogen Receptor 1). (wikipedia.org)
It is hypothesized that estrogen stimulation of ERα may trigger the release of growth factors, such as epidermal growth factor or insulin-like growth factor-1, which in turn regulate bone development and maintenance. (wikipedia.org)
Cellular1
In an extension of this work, we are also exploring the differential upregulation of various cellular genes by two different HIFs, HIF-1 and HIF-2. (nih.gov)
Expression1
Starr LA, McKay LE, Peter KN, Seyfarth LM, Berkowitz LA, Caldwell KA , Caldwell GA . Attenuation of Dopaminergic Neurodegeneration in a Parkinson's Model through Regulation of Xanthine Dehydrogenase (XDH-1) Expression by the RNA Editase, ADR-2. (neurotree.org)
Cells3
RINm5F cells (2.0 × 10 5 cells/400 μl RPMI-1640) were treated for 6, 12, or 24 h with IL-1 (10 units/ml), NMMA (2 mmol/l), and tunicamycin (2 μg/ml), as indicated. (medscape.com)
5. Murine gammaherpesvirus 68 reactivation from B cells requires IRF4 but not XBP-1. (nih.gov)
Most experts currently believe that HIV type 1 (HIV-1) protease inhibitors (PIs) and nucleoside reverse transcriptase inhibitors (NRTIs), especially stavudine and zidovudine, are implicated. (medscape.com)
Genes2
Factor de transcripción con cremallera de leucina básica que regula la expresión de GENES DEL COMPLEJO HLA CLASE II a través de su unión a una secuencia conservada de ADN denominada caja X en la región promotora. (bvsalud.org)
Regula la expresión de genes implicados en la RESPUESTA A PROTEÍNAS DESPLEGADAS. (bvsalud.org)
Cell3
Using a biochemical assay of membrane integrity, the effects of 6-, 12-, and 24-h exposures to IL-1 on RINm5F cell viability were examined. (medscape.com)
As shown in Fig. 2 A , a 24-h incubation with IL-1 results in a 23% reduction in RINm5F cell viability. (medscape.com)
NO is the mediator of cytokine-induced β-cell death, as IL-1-induced nitrite production correlates temporally with cell death (Fig. 2 B ) and NMMA prevents IL-1-induced β-cell death. (medscape.com)
Addition2
In addition, because coal fires can exceed 1, degrees F, most fill material burns away, leaving more gaps. (desenzanoloft.it)
Fluoride is anticariogenic and is recommended by the U.S. Public Health Service for addition to drinking water at a concentration of 0.7-1.2 ppm, such that an average of 1 mg F − is consumed per day [ Centers for Disease Control and Prevention (CDC) 1995 ]. (nih.gov)
Infection1
This is believed to be nearly always due to HSV-1 infection. (desenzanoloft.it)
Article1
1] This article addresses both lipohypertrophy and lipoatrophy, with a focus on the morphologic changes and underlying pathophysiology of HIV-associated lipodystrophy. (medscape.com)
Form1
Reconnection processes taking place at/between the coronal loops that form the XBP have been proposed as a possible explanation. (nasa.gov)
Products1
Eagle PLC offers a 2 year warranty on all Schneider Electric / Modicon products including the 140-XBP-010-00. (eagleplc.com)
Results2
This blog gives the results of a preliminary investigation of reduced-delay (reduced group delay) IIR filters based on my understanding of the concepts presented in a recent interesting blog by Steve Maslen [1]. (dsprelated.com)
Results Our outcomes indicated that ER tension and IRE-1/XBP-1 signaling are triggered in LPS-induced ALI. (bassresearch.com)
Days1
8 hr 10 min per day, i.e per XBP 4 days TIMING ====== 15, 16, 17 & 18 July 2003 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ CDS (SOHO) ~~~~~~~~~~~~ ================================ Use existing NIS & GIS sequences with some small changes Acronym Study: ID Var BP_W_1 13 22 BP_W_2 266 1 BP_W_3 267 1 SPECT_1 G2AL BP_W_1: context rasters at beginning, middle and end of the time series. (nasa.gov)
Sequences1
Sequences to be run and their duration ====================================== ======================================== BP_W_1 x 2 Context image: == 21 min. (nasa.gov)
Stock1
The Schneider Electric / Modicon 140-XBP-010-00 is in stock and ready to ship from Eagle PLC. (eagleplc.com)
Continuous1
The goal of interpolation is to increase the sample rate such that the new (interpolated) sample values are close to the values of the continuous signal at the sample times [1]. (dsprelated.com)
Page1
Informational [Page 1] RFC 3149 MGCP Business Phone Packages September 2001 3. (ietf.org)
Development1
Maslen's development of a reduced-delay 2nd-order IIR filter begins with a traditional prototype filter, HTrad, shown in Figure 1(a). (dsprelated.com)