In neurons, murine ortholog Wwp1 and its homolog Wwp2 control polarity acquisition, formation, and branching of axons, as well as migration of newly born nerve cells into the cortical plate. (wikipedia.org)
WW domain-containing protein-1 and -2 (WWP1 and WWP2) are evolutionarily conserved HECT type E3 ubiquitinligases in eukaryotes, and the C. elegans ortholog of WWP1/2 plays key roles in neuronal functions such as axon guidance and synaptic transmission. (uni-goettingen.de)
Die eukaryotischen "WW-Domänen enthaltenen Proteine 1 und 2" (WWP1 und WWP2) sind evolutionär konservierte E3 Ubiquitin Ligasen die eine HECT-Domäne aufweisen. (uni-goettingen.de)
Gene2
NEDD4-like E3 ubiquitin-protein ligase WWP1 is an enzyme that in humans is encoded by the WWP1 gene. (wikipedia.org)
Analysen von Gehirnen von Mäusen, mit einer spezifischen und begrenzten Ausschaltung der Gene für WWP1/2 im Gehirn, in vivo und in vitro zeigten, dass WWP1 und 2 entscheidend für die Etablierung der Polarität von Nervenzellen sind. (uni-goettingen.de)
Neurons1
These findings are consistent with the synaptic localization of WWP1/2 in mature neurons and indicate that WWP1/2 may be involved in synaptic transmission and synaptogenesis. (uni-goettingen.de)
Proteins1
Furthermore, I screened for binding proteins of WWP1/2 in the brain and identified WWP1/2 as specific E3 ligases for the synaptic scaffold proteins liprin-α3 and shank1a. (uni-goettingen.de)
Signaling pathway1
Thus I propose that WWP1/2 contribute to neuronal polarization, probably by regulating the SAD kinase signaling pathway. (uni-goettingen.de)
Polarity1
In analyses of brain specific conditional WWP1/2 deficient mice in vivo and in vitro, I found that WWP1/2 are critical for the establishment of neuronal polarity - the specification of a single axon and multiple dendrites, which is an essential step during neuronal development. (uni-goettingen.de)
Development1
Together, my findings show that WWP1/2 plays multiple roles in neuronal development and synaptic function in the mammalian brain. (uni-goettingen.de)
Encoded by the WWP1 gene1
NEDD4-like E3 ubiquitin-protein ligase WWP1 is an enzyme that in humans is encoded by the WWP1 gene. (wikipedia.org)
PTEN3
In particular, the WWP1 protein binds to an enzyme called PTEN. (medlineplus.gov)
Binding of the WWP1 protein to the PTEN enzyme impairs the PTEN enzyme's ability to dimerize, impairing the enzyme's activity. (medlineplus.gov)
Using human cells and mice bred to develop cancer, the researchers found that an enzyme known to promote cancer growth, called WWP1, played an important role in interfering with PTEN function. (consciouslifenews.com)
KLF151
In line with the Kobe analysis, the proteins WWP1 and KLF15 are what causes muscle atrophy. (ouzeritsitsanis.com)
Enzyme1
The ubiquitin conjugating activity of GST-WWP1 was validated through its ability to catalyse the generation of polyubiquitin chains in the presence of the E1 activating enzyme His-UBE1, the E2 conjugating enzyme His-UBE2L3 (UbcH7) (several E2s were tested, data generated with this E2 is provided by way of example) and ubiquitin. (ubiquigent.com)
HECT1
translocated to, 1) as well as HACE1 (HECT Domain And Ankyrin Repeat Containing E3 Ubiquitin Protein Ligase 1), WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1), 14 HMGN1 )High Mobility Group Nucleosome Binding Domain 1), PLAU (plasminogen activator, urokinase), and PSAP (prosaposin). (pharmamodels.net)
Amino acids1
WWP1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human WWP1. (ecmbio.com)
Variants2
Researchers describe these variants as "gain-of-function" because they appear to enhance the activity of the WWP1 protein. (medlineplus.gov)
20. Ubiquitin Ligase Activities of WWP1 Germline Variants K740N and N745S. (nih.gov)
Cancers1
WWP1 has been implicated as an oncogene as it is overexpressed in prostate and breast cancers, and knockdown of this ligase reduces cell proliferation and causes apoptosis. (ecmbio.com)
Degradation1
In addition, WWP1 has been shown to negatively regulate transforming growth factor-β (TGFβ) signaling by targeting TGFβ receptor 1, Smad2, and Smad4 for ubiquitin-mediated degradation. (ecmbio.com)
Lane3
Incubation of GST-WWP1 for 60 minutes at 37°C in the presence of ubiquitin, His-UBE1, His-UBE2L3 and ATP (Lane 1) was compared alongside two control reactions with either ATP (Lane 2) or GST- WWP1 (Lane 3) excluded from the reaction. (ubiquigent.com)
Ubiquitin conjugates were identified by Western blotting using an anti-ubiquitin conjugate antibody and these were observed only in the presence of both ATP and GST-WWP1 (with the exception of one species of approximately 140 MW observable in lane 3). (ubiquigent.com)
Western blot analysis of WWP1 expression in adult mouse liver (lane 1) and human prostate adenocarcinoma (PC3) cells (lane 2). (ecmbio.com)
Expression1
Abnormal expression of WWP1 in chronic lymphocytic leukemia and its clinical significance]. (cdc.gov)
Cells2
In neurons, murine ortholog Wwp1 and its homolog Wwp2 control polarity acquisition, formation, and branching of axons, as well as migration of newly born nerve cells into the cortical plate. (wikipedia.org)
The antibody detects a 110 kDa* protein corresponding to the apparent molecular mass of WWP1 on SDS-PAGE immunoblots of adult mouse liver and human PC3 cells. (ecmbio.com)
Family2
Flasza M, Gorman P, Roylance R, Canfield AE, Baron M (2002) Alternative splicing determines the domain structure of WWP1, a Nedd4 family protein. (ubiquigent.com)
This sequence is highly conserved in rat and mouse WWP1, and has low homology to other NEDD4-like family members. (ecmbio.com)