Wild type p53 cyclin dependent kinase inhibitor p21. Medical search. Frequent questions

In response to DNA-damaging agents, the wild-type p53-activated fragment 1 (WAF1 also known as p21) is an important downstream effector in the p53-specific growth arrest pathway. (lu.se)
The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
Purpose: The study investigated whether basal, constitutive levels of p21(WAF1/CIP1) protein in murine carcinoma are related to in vivo tumor radioresponse. (elsevierpure.com)
The study is based on recent observations demonstrating that in vitro cancer cell lines are resistant to cytotoxic drugs when they express high basal levels of p21(WAF1/CIP1) protein, and that the loss of the p21 gene in the HCT116 human colorectal cancer cell line results in increased radioresponse of xenografts derived from that cell line. (elsevierpure.com)
Methods and Materials: Protein levels of p21(WAF1/CIP1), p53, bax, and bcl-2 were determined in 8 carcinomas (3 mammary carcinomas designated MCa-4, MCa-29, and MCa-35, 2 squamous cell carcinomas designated SCC-IV and SCC-VII, ovarian adenocarcinoma OCa-I, hepatocarcinoma HCa-I, and adenosquamous carcinoma ACa SG) syngeneic to C3Hf/Kam mice using Western blot analysis. (elsevierpure.com)
Results: Protein levels of these oncogenes varied among tumors, with p21(WAF1/CIP1) showing the greates variation: its mean densitometric value ranged from 1 to 19. (elsevierpure.com)
Tumor radioresponse correlated significantly (R = 0.77, p = 0.02) only with the magnitude of p21(WAF1/CIP1) expression: tumors with high levels of p21(WAF1/CIP1) were less radiocurable than those with lower levels. (elsevierpure.com)
Despite a strong trend to correlation, (p = 0.15), p21(WAF1/CIP1) expression did not correlate significantly with radiation-induced apoptosis, which suggested that p21(WAF1/CIP1) influenced tumor radioresponse by mechanisms beyond that of apoptosis induction. (elsevierpure.com)
Conclusion: Our findings showed that murine tumors exhibit wide variation in constitutive levels of p21(WAF1/CIP1) which had a significant relationship with tumor radioresponse: tumors with high levels of p21(WAF1/CIP1) were less radiocurable than those with lower levels. (elsevierpure.com)
These findings support the concept that p21(WAF1/CIP1) is a major determinant of tumor radioresponse in vivo, and may have important clinical implications. (elsevierpure.com)
The pretreatment assessment of p21(WAF1/CIP1) protein could serve as a useful predictor of radiotherapy outcome and may assist in selecting an effective treatment modality. (elsevierpure.com)
Protein levels of CDKN1A -encoded p21 CIP1/WAF1 were determined by western blotting and the importance of p53 was shown by immunofluorescence and by a loss-of-function approach. (biomedcentral.com)
Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
EBF3 bound directly to p21 cip1/waf1 promoter and regulated transcription from both p21 cip1/waf1 and p27 kip1 promoters in reporter assays. (aacrjournals.org)
In view of the importance that the checkpoint G1 / S in the viral replication plays, it is not surprising that HIV-1 proteins, Indeed, as has been shown that the activity t of G1 / S Our own studies, we observed the activity cyclin E/CDK2 kinase complexes, the t in HIV-infected cells of a fa erh ht Is latent because of the loss of natural CDK inhibitor p21/waf1. (micrornalibrary.com)
Cdk inhibitor p21/waf1 is normally cellular p53 in Ren induced stress and regulates the G1 / S transition by inhibiting the activity of t of cyclin / cdk complexes. (micrornalibrary.com)
Studies from our laboratory have shown that an HIV-infected T-cells of F Latent expression of p21/waf1 is not to breach the host cell They induce. (micrornalibrary.com)
The lack of expression of p21/waf1 was on physical and functional interaction of Tat with p53, which then caused due no inactivation of p53. (micrornalibrary.com)

p53 is a transcription factor that participates in cell cycle checkpoint processes and apoptosis. (lu.se)
p53 is critical for DNA repair and apoptosis. (umich.edu)
Our simulations show that, if HMGB1 is overexpressed, then the oncoproteins CyclinD/E, which regulate cell proliferation, are overexpressed, while tumor suppressor proteins that regulate cell apoptosis (programmed cell death), such as p53, are repressed. (biomedcentral.com)
Moreover, the models also predict that mutations of RAS, ARF and P21 in the context of HMGB1 signaling can influence the cancer cell's fate - apoptosis or survival - through the crosstalk of different pathways. (biomedcentral.com)
The HMGB1 signal transduction can influence the cell's fate by two important processes - apoptosis and cell proliferation - which are regulated respectively by the proteins p53 and CyclinE, acting in two different signaling pathways. (biomedcentral.com)
The protein p53 is one of the most important tumor suppressor proteins: its activation can lead to cell cycle arrest, DNA repair, or apoptosis. (biomedcentral.com)
NQ induced apoptosis in HepG2 cells by activating p53-ROS crosstalk and induces epigenetic modifications leading to inhibited proliferation and cell cycle arrest. (molcells.org)
Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
It was identified that propofol inhibited the viability and invasion, but promoted apoptosis of HGC‑27 and AGS cells in a dose‑dependent manner. (spandidos-publications.com)
A hypoxic environment devoid of nutrients prevents the cell undergoing energy dependent apoptosis and cells become necrotic. (bmj.com)
The key regulator of this process, hypoxia inducible factor 1 (HIF-1), can initiate apoptosis by inducing high concentrations of proapoptotic proteins, such as BNIP3, and can cause stabilisation of p53. (bmj.com)
The p53-inducible gene 3 (PIG3) was originally indentified by Polyak et al in the analysis of p53-induced genes related to the onset of apoptosis ( 1 ). (ijbs.com)
In some p53 mutants, induction of cell cycle arrest, but not apoptosis was found to be associated with a lack of induction of PIG3 expression ( 2 ). (ijbs.com)

Mutations of p53 tumor suppressor gene increase with tumor progression in colorectal cancers. (nih.gov)
The basic unit of the p53 protein consists of three major functional domains such as an N-terminal transactivation domain (TAD), a core DNA-binding domain (DBD)-the main target for mutations, and a C-terminal regulatory domain (CTD) ( Figure 1 A) [ 2 ] . (encyclopedia.pub)
In human cancers, specific residues of p53 are mutated at a high frequency, and those mutations are known as hotspot mutations. (bvsalud.org)
Mutations of p53 are associated with many forms of human malignancy. (umich.edu)
Mutations in cyclin-dependent kinase inhibitors controlling the G1 phase of the cell cycle are prevalent in various cancers. (koreamed.org)
Mutations of p53 occur at a frequency of 50% or higher in many different cancer types [ 13 ]. (biomedcentral.com)
The majority of classical LFS families harbour germline mutations of the p53 tumour suppressor gene. (bmj.com)
These had all been previously screened for the presence of p53 mutations in exons 2 and 4-11 by single strand conformational polymorphism (SSCP) analysis and DNA sequencing as previously described. (bmj.com)
Different cancer types tend to depend on a limited number of 'driver' oncogene mutations. (cancerquest.org)

Both NUGC-4-mutant-p53 cell types expressed epithelial-mesenchymal transition (EMT)-related proteins. (bvsalud.org)
Furthermore, NUGC-4-mutant-p53-R175H cells showed less attachment to the extracellular matrix and greater expression of EMT-related proteins than NUGC-4-mutant-p53-R273H cells. (bvsalud.org)
In addition, numerous important cellular proteins, such as B-cell lymphoma-2 homologous antagonist/killer, Myc proto-oncogene protein, cyclin-dependent kinase inhibitor 1B, DNA replication licensing factor MCM-7, retinoblastoma 1 and Annexin A1, are degenerated through the UBE3A-mediated ubiquitin proteasome pathway ( 7 ). (spandidos-publications.com)
The crosstalk is regulated by tumor suppressor proteins, including ARF, P21 and FBXW7, which are also frequently mutated in many cancers. (biomedcentral.com)
8 9 This lack of an absolute phenotype:genotype concordance could be attributed to incomplete screening of the p53 gene, inactivation of the p53 protein through interaction with other cellular proteins or viruses, or defects in other genes involved in p53 mediated cell cycle regulatory pathways. (bmj.com)
Preeminent among these proteins are the cyclin dependent kinases, which upon binding to cyclins, phosphorylate numerous targets to trigger cell cycle progression. (smadpathway.com)
These proteins, including Aurora A and B, are serine/threonine kinases that are essential for cell division activities such as spindle construction, chromosome segregation, and cytokinesis. (smadpathway.com)

At the molecular level, pitavastatin induced expression of the cyclin dependent kinase (CDK) inhibitor p21 in a cholesterol independent manner, blocked repressive phosphorylation of the Retinoblastoma tumor suppressor protein at CDK targeted sites, and reduced expression of E2F target genes required for progression through the G1/S boundary. (oncotarget.com)
Mutant p53 promotes tumor progression through the gain-of-function (GOF) mechanism. (bvsalud.org)
p53, mdm-2, p21, and mib-1 expression were not significantly associated with response to chemotherapy, time to progression, or overall survival in the whole patient population or in the docetaxel group. (lu.se)
This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)
Mice lacking the tumor suppressors p16(Ink4a) (Cdkn2a, cyclin-dependent kinase inhibitor 2a), p19(Arf) (an alternative reading frame product of Cdkn2a,), and p27(Kip1) (Cdkn1b, cyclin-dependent kinase inhibitor 1b) result in malignant progression of epithelial cancers, sarcomas, and melanomas, respectively. (koreamed.org)
18-20 p16 INK4a shares functional similarities with p53 in that it blocks progression through the cell cycle at G1/S by inhibiting CDK 4/6 mediated phosphorylation of Rb. (bmj.com)
These results suggest that a p53-dependent pathway monitors telomere capping after DNA replication and delays G2/M progression in the presence of unprotected telomeres. (ox.ac.uk)
Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)
New sequencing technologies have shown that a large number of aberrantly expressed lncRNAs are associated with multiple cancer types and indicated they have emerged as an important class of pervasive genes during the development and progression of cancer. (biomedcentral.com)

Cells from breast cancer tumors were found to have elevated levels of CDK7 and Cyclin H when compared to normal breast cells. (wikipedia.org)
THZ1 was tested on HER2-positive breast cancer cells and exhibited high potency for the cells regardless of their sensitivity to HER2 inhibitors. (wikipedia.org)
Studies with pitavastatin in primary patient derived tumor cells and established PDA lines, revealed dose dependent growth inhibition. (oncotarget.com)
MATERIALS AND METHODS: This study examined the differences in the metastatic potential of wild-type, mutant-p53-R175H, and mutant-p53-R273H NUGC-4 gastric cancer cells in vitro and in vivo. (bvsalud.org)
RESULTS: NUGC-4-mutant-p53-R175H cells showed significant cell proliferation, healing and invasive abilities in proliferation, wound healing and invasion assay, respectively, compared to wild-type and mutant-p53-R273H cells. (bvsalud.org)
Regarding the peritoneal dissemination model, NUCG-4-mutant-p53-R175H and NUCG-4-mutant-p53-R273H cells demonstrated less frequent formation of dissemination nodules than NUGC-4-empty cells. (bvsalud.org)
CONCLUSION: Our results suggest that differences in the p53 status, even in the hotspot mutation site, affect not only the characteristics of the cells but also the metastatic ability of gastric cancer. (bvsalud.org)
p27 is a cyclin-dependent kinase inhibitor with many putative functions in normal and neoplastic cells. (umich.edu)
Precis: Pulsed high doses versus sustained low doses of the p53-MDM2 inhibitor HDM201 elicit a pro-apoptotic response from wild-type p53 cancer cells, offering guidance to current clinical trials with this and other drugs that exploit the activity of p53. (bace-signal.com)
Continuous exposure of HDM201 led to induction of p21 and delayed accumulation of apoptotic cells. (bace-signal.com)
The overexpression of miRNA‑375 significantly increased caspase‑3 and caspase‑9 activities, induced B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein, tumor protein 53 and cyclin‑dependent kinase inhibitor 1 protein expression and suppressed cyclin D1 and survivin protein expression in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
Unlike their wild-type counterparts, human and mouse cells lacking p53 or p21 progress into mitosis prematurely with persisting uncapped telomeres. (ox.ac.uk)
Uncapped telomeres that persist in mitotic p53-deficient cells are shorter than average and religate to generate end-to-end fusions. (ox.ac.uk)
Notably, terpinen-4-ol was able to increase p53 levels in A549 and CL1-0 cells. (hindawi.com)
Induction of p53 by roscovitine has in many cell lines.54 60 A Similar scheme has been exposed to p53 in SH SY5Y cells roscovitine and CR8 observed have been reported. (micrornalibrary.com)
The use of different cell lines have shown that Mcl NB 1 downregulation of roscovitine and CR8 was induced in cells in which p53 is absent, mutated or had undergone a significant increase in the size E at result of the reproduced by Ltigung of 3 exons. (micrornalibrary.com)
Close Of course, we used p53 / p53 / and HCT116 wild-type cells.63 two roscovitine and CR8 were foreign Mcl a down-regulation sen, Also in theTat further stimulates the phosphorylation of RNA Pol II hyperphosphorylated. (micrornalibrary.com)
More recently, two studies point toward Dicer-dependent nuclear RNAi in mammals by demonstrating that nuclear, chromatin-associated Dicer impairs expression of the microtubule-binding protein Doublecortin in mouse adult neural stem cells [ 14 ] and transactivation of the human secreted frizzled-related protein 1 promoter in cholangiocarcinoma cells [ 15 ]. (prolekarniky.cz)
Propofol could inhibit Bcl‑2 and MMP9 expression, and increase P21 expression in GC cells. (spandidos-publications.com)
Additionally, the effects of transfection with circ‑PVT1 small interfering RNA (si‑circ‑PVT1) on HGC‑27 and AGS cells could be reversed by treatment with miR‑195‑5p inhibitor. (spandidos-publications.com)
In GC, Yang et al ( 7 ) reported that propofol suppressed the proliferation of SGC-7901 and MGC-803 cells by promoting inhibitor of growth 3 ( 7 ). (spandidos-publications.com)
Embryogenesis depends on the timely proliferation of progenitor cells and their subsequent differentiation into multiple cell types. (biologists.com)
For example, in the developing nervous system of mouse embryos,progenitor cells proliferate and sequentially give rise to different types of cells by changing their differentiation competency. (biologists.com)
Without Hes genes such as Hes1 , however, progenitor cells prematurely differentiate into certain types of neurons only, and are depleted before they have proliferated sufficiently and generated all neuronal and glial cell types. (biologists.com)
Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
To test the utility of the sensor as a screening tool cells expressing the sensor were exposed to a known inhibitor of O-glycosylation extension or siRNAs targeting factors known to alter glycosylation efficiency. (sunolmolecular.com)
Hypoxia stimulates hypoxia inducible factor (HIF)-dependent expression of CXCL12 and KITL promoting mobilization from the bone marrow and recruitment to primary tumor and metastatic sites of CXCR4 + CD11b + bone marrow-derived cells and KITbCD11b + cells assisting vasculogenesis and metastasis respectively (Kuonen et al. (biomedcentral.com)
Such treatment was associated with upregulation of invasion- and inflammation-promoting soluble factors, such as matrix metalloproteinase 2 (MMP2), its activator MMP14, tissue inhibitors of matrix metalloproteinase 2 (TIMP2), chemokine ligand 2 (CCL2), and urokinase-type plasminogen activator (uPA), the latter two being linked to the recruitment of the monocytic cells. (biomedcentral.com)

Cyclin-dependent kinase 7, or cell division protein kinase 7, is an enzyme that in humans is encoded by the CDK7 gene. (wikipedia.org)
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. (wikipedia.org)
Interestingly, in eight cases with p53 wild-type gene, 6 cases (75%) showed a marked down-regulation of p14ARF mRNA, and three cases (37.5%) over-expressed MDM2 mRNA. (nih.gov)
Only one case with wild-type p53 gene showed normal level expression of p53 regulatory-factors (p33ING1, p14ARF, and MDM2). (nih.gov)
The tumor suppressor p53, encoded by the TP53 gene and known as "the guardian of the genome", performs a variety of functions in cancer prevention. (encyclopedia.pub)
It has been recognized that 50% of cancer patients acquire certain types of TP53 gene alterations [ 9 ] [ 10 ] . (encyclopedia.pub)
BACKGROUND/AIM: P53 is the most frequently mutated tumor suppressor gene among all cancers. (bvsalud.org)
The protein product of the murine double minute gene 2 (mdm-2) plays a central role in the regulation of p53. (lu.se)
Herpes virus thymidine kinase (HSV-tk) is used in suicide gene therapy. (umich.edu)
The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. (cancerindex.org)
E ditor -The Li-Fraumeni syndrome (LFS) is a rare familial cancer syndrome that predisposes gene carriers to the development of diverse early onset malignancies, including soft tissue sarcomas, osteosarcomas, adrenocortical carcinomas, brain tumours, breast carcinomas, and leukaemia, 1-3 with other cancer types occurring less frequently. (bmj.com)
p16 INK4a is a candidate gene that could account for the cancer predisposition in p53 wild type LFS families. (bmj.com)
In view of the comparable biological and phenotypic features of p53 and p16 INK4a inactivation, we proposed that germline inactivation of the p16 INK4a gene could account for the predisposition to cancer development in a proportion of LFS families that harboured wild type p53 . (bmj.com)
Both p53 wild type and p53 mutant samples were included in the p16 INK4a analysis to determine whether germline alterations of one gene precluded the occurrence of alterations of the other. (bmj.com)
Vitamin D is also known to exert rapid effects that are not dependent on gene transcription [ 5 ]. (biomedcentral.com)
The bands are characterized by low gene density, contain predominantly tissue-specific genes, and are represented by silent chromatin types in various tissues. (sdbonline.org)
The p53-inducible gene 3 (PIG3) recently has been reported to be a new player in DNA damage signaling and response, but the crucial mechanism remains unclear. (ijbs.com)
The larger the gene name, the more frequently that gene is defective in that cancer type. (cancerquest.org)
As an example TP 53 refers to the gene and p53 refers to the protein. (cancerquest.org)
HER2/neu (also called ERB B2 ) is the gene that encodes the human epidermal growth factor receptor type 2. (cancerquest.org)

In this study, we examined the expressions of p33ING1, p14ARF, MDM2 and p21WAF1 mRNA in 25 advanced colorectal cancers by quantitative RT-PCR method, and compared the expression levels of p33ING1, p14ARF, p21WAF1 and MDM2 in relation to p53 status in the tumors. (nih.gov)
Activation of p53 by inhibitors of the p53-MDM2 interaction is being pursued as a therapeutic strategy in p53 wild-type cancers. (bace-signal.com)
Here we report distinct mechanisms by which the novel, potent, and selective inhibitor of the p53-MDM2 interaction HDM201 elicits therapeutic efficacy when applied at various doses and schedules. (bace-signal.com)
Discrete, stochastic simulations show that p53 and MDM2 oscillations continue even after 10 hours, as observed by experiments. (biomedcentral.com)

p21 and other tumor suppressor genes' inactivation are therefore most likely involved in the first steps of the cancer model, with later stages possibly involving protooncogene activation and inflammation. (medscape.com)
Interestingly, the 1p36 region is frequently deleted not only in NB, but also in other human cancer types, including those of neural, epithelial and hematopoietic origin, indicating that the same tumor suppressor genes might be involved in a broad range of human cancers [ 11 ]. (biomedcentral.com)
These events are dependent on the proper levels of transcription and translation of certain genes. (cancerquest.org)

Fifteen of 25 colorectal cancers (60%) showed abnormal accumulation of p53 protein in the nucleus, and the remaining 10 colorectal cancers (40%) were negative for p53 immunostaining. (nih.gov)
The potential role of p53 as a specific target in modern therapies against cancers is being widely discussed. (encyclopedia.pub)
p16 is a cyclin-dependent kinase inhibitor that has shown prognostic utility in some human cancers. (umich.edu)

p53, mdm-2, p21, and mib-1 expression were assessed by immunohistochemical methods in primary tumors derived from 134 patients who took part in a randomized multicenter trial comparing docetaxel to sequential methotrexate and 5-fluorouracil (MF) in advanced breast cancer. (lu.se)
Interestingly, tumors with both negative mdm-2 and p21 expression, irrespective of p53 status, had a high response rate to docetaxel but no response to MF. (lu.se)
their variation was within a 5-fold range, and the level of p53 was similar in 6 of 8 tumors. (elsevierpure.com)
However, it remains unknown whether inactivation of EBF3 locus also occurs in other types of tumors and what functions of EBF3 underlie EBF3-mediated tumor suppression. (aacrjournals.org)

Mutant p53 was unable to downregulate CDK7 activity and mutant p21 had no effect on downregulation, indicating that p53 is responsible for negative regulation of CDK7. (wikipedia.org)
Gain of Function Mutant p53 in Cancer" Encyclopedia , https://encyclopedia.pub/entry/35066 (accessed December 03, 2023). (encyclopedia.pub)
In contrast, liver metastases were more frequent and greater in number in NUCG3-mutant-p53-R175H than in the other cell lines. (bvsalud.org)
Additionally, forced expression of NBPF1 in two p53-mutant neuroblastoma cell lines also resulted in a G1 cell cycle arrest and CDKN1A upregulation. (biomedcentral.com)
The antibody should recognize human wild-type and mutant p53. (abcam.com)

The growth suppressor p53 has been shown to interact with cyclin H both in vitro and in vivo. (wikipedia.org)

Here, we report that telomere capping is monitored at the G2/M transition by the p53/p21 damage response pathway. (ox.ac.uk)
Wu M, Sun Y, Xu F, Liang Y, Liu H, Yi Y. Annexin A2 Silencing Inhibits Proliferation and Epithelial-to-mesenchymal Transition through p53-Dependent Pathway in NSCLCs. (jcancer.org)

Interestingly, kuanoniamine C-induced cell death and downregulation of GRP78 expression was regulated by p53 signaling. (bvsalud.org)
In breast cancer patients, it is unclear whether measuring p53, mdm-2, or p21 expression provides information on how patients will respond to chemotherapy. (lu.se)
Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E. (uiowa.edu)
Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)
Meanwhile, miR‑195‑5p inhibitor reversed the si‑circ‑PVT1‑induced low expression of ETS1. (spandidos-publications.com)
The IR-increased expression of PIG3 is p53 dependent ( 7 ). (ijbs.com)

Recently, a growing body of evidence that r is given On a different cyclin / CDK complex, n Namely Cyclin E/CDK2 in Tat-activated transcription. (micrornalibrary.com)
Antagonists such as FGF and EGF, and cellular Ren stress signals act through mitogen-activated protein kinases, phosphorylation of a region that induce DNA binding and Smad transcription bridged. (micrornalibrary.com)

We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)

Addition of wild type p53 was found to heavily downregulated CAK activity, resulting in decreased phosphorylation of both CDK2 and CTD by CDK7. (wikipedia.org)
In kinase assays, induces phosphorylation of the CTD by CDK9 fact n TIG, there N-terminal arginine-rich motif and deed. (micrornalibrary.com)
The linker Smad-dependent cyclin is Independent kinases may need during the G1 cell cycle and MAPK phosphorylation by GSK3 action completed. (micrornalibrary.com)
Importantly, one other individual meats directly influence Aurora T localization, and phosphorylation of conserved residues in the C terminus of INCENP greatly raises Aurora B kinase activity. (smadpathway.com)

It is a key effector of cellular senescence downstream of the tumor suppressor p53. (umich.edu)

In 2017 CT7001, an oral CDK7 inhibitor, started a phase 1 clinical trial. (wikipedia.org)

Western blotting was performed to detect the protein levels of cyclin‑dependent kinase inhibitor P21 (P21), B‑cell lymphoma‑2 (Bcl‑2), matrix metalloproteinase 9 (MMP9) and E26 oncogene homolog 1 (ETS1). (spandidos-publications.com)

T transition (nonsense mutation) at the first nucleotide of codon 298 (exon 8) in one p53-negative case, and a frame shift mutation on exon 7 in another p53-negative case. (nih.gov)
Cyclin E/CDK2 has been shown to play an R Important by regulating the release of factors, including normal Rb E2F sequestered in the transition from G1 / S. (micrornalibrary.com)

Along with Cdk1/cyclin N, members of the Aurora/Ipl1 kinase family are also crucial regulators of mitosis. (smadpathway.com)

Cyclin E/CDK2 is the main cyclin / CDK complex, whose maximal activity is t observed in the sp Th G1 / S boundary. (micrornalibrary.com)

Selected oncogenes that have been associated with numerous cancer types are described in more detail below. (cancerquest.org)

Here, we generated knockout mouse models for each of these three cyclin-dependent kinase inhibitors using engineered nucleases. (koreamed.org)
A schematic of the Cdkn1b locus with single guide RNA target nucleotides (blue) and the amino acid sequences (purple) of the wild-type (upper) and knockout (lower) mice are shown. (koreamed.org)

The exposure SU11274 to this substance leads to stabilization and accumulation of p53 and Hdm2.62 Despite the regulations in force p53, Mcl 1 levels remained constant. (micrornalibrary.com)

Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)
Considering this genetic defect and the frequent genomic alterations of the NBPF1 locus in several cancer types, we hypothesized that NBPF1 is a tumor suppressor. (biomedcentral.com)

Cooperation between oncogenic Ras and wild-type p53 stimulates STAT non-cell autonomously to promote tumor radioresistance. (uiowa.edu)

Our results indicated that terpinen-4-ol elicited a dose-dependent cytotoxic effect, as determined by MTT assay. (hindawi.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Many studies have found evidence of crosstalk between the two signaling pathways involving p53 and CyclinE [ 15 ]. (biomedcentral.com)

These results suggest that co-treatment with bortezomib and kuanoniamine C is a novel therapeutic strategy for the treatment of osteosarcoma that enhances bortezomib-dependent cell death by the downregulation of GRP78, and this combination selectively targets the major cell population of osteosarcoma, which expresses wild-type p53. (bvsalud.org)

13 p16 INK4a is a cyclin dependent kinase inhibitor that is frequently mutated or deleted in many human cancer cell lines 14-16 and some sporadic malignancies, including sarcomas, breast cancer, leukaemia, and brain tumours, which are all component tumours of LFS. (bmj.com)

HDAC8 inhibitor Ufd1 was proved to be required for Survivin ubiquitination, and for the localization of Aurora and Survivin W to centromeres. (smadpathway.com)

Although steady-state conditions revealed no increase in primitive cell proliferation in p21-null mice, a significantly larger fraction of quiescent neural precursors was activated in the hippocampus and subventricular zone after brain ischemia. (rupress.org)
Recent studies have found that overexpression of the High-mobility group box-1 (HMGB1) protein, in conjunction with its receptors for advanced glycation end products (RAGEs) and toll-like receptors (TLRs), is associated with proliferation of various cancer types, including that of the breast and pancreatic. (biomedcentral.com)

An E1/E3 deleted adenovirus type 5 vector expressing cre recombinase under CMV promoter control. (umich.edu)
Lyoma virus enhancer, HSV-tk enhancer and promoter, HSV thymidine kinase. (umich.edu)
E1/E3 deleted, CMV promoter, expressing human p53 wild type. (umich.edu)

Crystallization and Structural Determination of ABLT315I:AP24534 The kinase domain of murine ABLT315I was coexpressed with YopH protein tyrosine phosphatase in E. coli as described previously and purified in the clear presence of AP24534 to near homogeneity using metal affinity, Mono Q, and measurement exclusion chroma tography. (smadpathway.com)

10-12 However, in the remaining "classical" LFS families, and in most LFS-L families, no alterations in p53 have been found. (bmj.com)

MiRNAs are abundant in many mammalian cell types and as extracellular circulating miRNAs. (micrornalibrary.com)

Responsible for such pivotal processes, p53 can deliver considerable damage if mutated, thus becoming "the guardian of the cancer cell" [ 8 ] . (encyclopedia.pub)
The DNA double-strand break (DSB) is considered to be the most severe type of DNA damage induced by ionizing radiation, and this form of DNA damage must be repaired immediately to prevent cell death. (ijbs.com)

HPV is a type of epithelial DNA virus, which has many subtypes, among which HPV6 and HPV11 cause CA ( 2 , 3 ). (spandidos-publications.com)

Mcl a down-regulation of roscovitine and CR8 is induced independently Ngig of p53 to regulate or MYCN amplification. (micrornalibrary.com)

Anatomy8

Organisms3

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes34

Disciplines and Occupations1

Information Science3

WAF119

Apoptosis15

Mutations9

Proteins7

Progression9

Cells28

Gene20

MDM24

Genes3

Cancers3

Tumors4

Mutant5

Vitro and in vivo1

Pathway2

Expression6

Transcription2

Vivo1

Phosphorylation4

Effector1

20171

Matrix metalloproteinase1

Transition2

Cdk11

CDK21

Oncogenes1

Knockout2

Accumulation1

Suppressor3

Stimulates1

Assay1

Pathways2

Downregulation1

Frequently mutated1

Ubiquitination1

Proliferation2

Promoter3

Murine1

Alterations1

Extracellular1

Damage2

Epithelial1

Regulate1