• Mechanistically, lncRNAs have been characterized as sponges with microRNAs (miRNAs) or circular RNAs (circRNAs) and act as competing endogenous RNAs (ceRNAs) to reduce the regulation of their target genes [ 15 ]. (biomedcentral.com)
  • Purpose: GG-H whole transcriptome array analysis suggested involvement of PININ (PNN) in the alternative splicing of multiple long non-coding RNAs (lncRNAs). (molvis.org)
  • Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
  • Alternatively spliced lncRNAs were detected with in situ hybridization with variant-specific RNA probes on human cornea sections. (molvis.org)
  • Conclusions: The data suggest PNN's role in the alternative splicing of a specific subset of lncRNAs might have a significant impact on the corneal epithelium. (molvis.org)
  • These regions are usually transcribed into long non-coding RNAs (lncRNAs). (biomedcentral.com)
  • Long non-coding RNAs (lncRNAs) have been demonstrated to be intensively involved in the development of various carcinomas, including glioblastoma multiforme (GBM). (cancerindex.org)
  • In situ hybridization analyses identify multiple locations of carbonic anhydrase expression in the larval mosquito. (silverchair.com)
  • Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)
  • They compete with endogenous nucleotides (dNTP/NTP) for incorporation into nascent DNA/RNA and inhibit replication by preventing subsequent primer extension. (plos.org)
  • Diverse KRAB-ZFPs recognize specific DNA sequences with the majority targeting endogenous retroviruses, ensuring their repression. (elifesciences.org)
  • Further mechanistic research indicated that circHERC1 acted as a competing endogenous RNA of miR-142-3p to relieve the repressive effect of miR-142-3p on its target HMGB1, activating the MAPK/ERK and NF-κB pathways and promoting cell migration and invasion. (biomedcentral.com)
  • Although Rab11a subcellular localization has been demonstrated by transgenic zebrafish [10], and molecular function of Rab11 proteins in primary cilia membrane assembly in Kupffer Vesicle has been studied [6], the developmental expression profile of Rab11 proteins in zebrafish remains elusive. (researchsquare.com)
  • however, the subcellular localization of the channels in HCs has not been fully resolved. (jneurosci.org)
  • Loss of PPan, but not interference with rRNA processing, led to an early downregulation of specific marker genes of the eye, including Rx1 and Pax6 , and of NCCs, such as Twist, Slug and FoxD3 . (silverchair.com)
  • While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
  • In humans, mutations in specific miRNA genes are associated with hearing loss. (biomedcentral.com)
  • All these studies have shown that spalt is activated in response to several signal transduction pathways in different tissues and developmental processes [ 11 ], and the expression patterns and functions of sall genes, together with the analysis of their regulation, indicate they cannot be universally assigned to a specific signaling pathway. (biomedcentral.com)
  • This method was used to measure the number of copies of repeated genes such as rRNA genes (2) and to measure whether specific genes were under-replicated during the replication process used in forming polytene chromosomes (3). (ddw-online.com)
  • The follicular status of F1 and F2 were further evaluated in terms of healthy and atretic conditions by investigating mRNA localization of identified genes. (biomedcentral.com)
  • Expression of stage-specific genes in follicles may be closely associated with their growth or atresia. (biomedcentral.com)
  • 3] In fact a quick BLAST search of Propionibacterium acnes genes for 16S ribosomal RNA (GenBank: AB097215.1) against M. tuberculosis shows an 88% identity within the first 100 hits. (j-alz.com)
  • qRT-PCR, western blotting and immunochemistry/In situ hybridization staining assays were performed to assess the expression of SNHG4, RRM2, TK1, AURKA, EZH2 and RREB1. (biomedcentral.com)
  • The interaction of circHERC1 with FOXO1 was verified by RNA pull-down, RNA immunoprecipitation (RIP) and western blot assays. (biomedcentral.com)
  • Noncoding RNAs such as circular RNAs (circRNAs) are abundant in the human body and influence the occurrence and development of various diseases. (biomedcentral.com)
  • Luciferase reporter assay and RNA immunoprecipitation assay were performed to verify the RNA-RNA interactions. (biomedcentral.com)
  • SUMOylation influences Bonus's subnuclear localization, its association with chromatin and interaction with SetDB1. (elifesciences.org)
  • The introduction of solid supports for DNA hybridisation/reassociations greatly broadened the range of applications of nucleic acid hybridisations, and provided the basis for solid-based methods being used today. (ddw-online.com)
  • METHODS The localization of VEGF-A, VEGF-C, VEGF-D, VEGF receptor (VEGFR)-1, VEGFR-2, and VEGFR-3 was examined in cancerous and adjacent benign prostate tissue from 52 subjects representing various grades of prostate cancer. (cdk-receptor.com)
  • Electrochemically triggered condensation, folding and assembly were simultaneously analysed using in situ dynamic light scattering, circular dichroism and UV absorbance spectroscopies. (bvsalud.org)
  • Dehydrated cells can be stored under these conditions for up to one month and must be rehydrated before being used in the in situ assay. (ice-hbv.org)
  • Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
  • DUX4‐fl transcription factor misexpression in skeletal muscle leads to disrupted RNA splicing, nonsense mediated decay (NMD), and protein degradation, possibly causing damaged associated molecular pattern (DAMP) release, which may elicit an immune response to targeted damaged muscles. (comprehensivephysiology.com)
  • Polyglutamine-binding protein 1 ( PQBP1 ) is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by severe cognitive impairment, but also by physical defects that suggest PQBP1 has broader functions in embryonic development. (xenbase.org)
  • Moreover, zebrafish is very well-suited for systematic analysis of gene expression patterns at transcript level by whole-mount in situ hybridization (WISH), and at protein level by immunohistochemistry/immunofluorescence (IHC/IF). (frontiersin.org)
  • Knockdown of Nedd4 binding protein three in producing primary hippocampal cultures alters axonal and dendritic branching Over the basis of our localization research, we cloned a functional N4BP3 interfering RNA con struct and performed transient N4BP3 KD experiments in main hippocampal neurons throughout the periods of axonal and dendritic development. (cret-signal.com)
  • Transfer RNA-mediated posttranslational protein modification by arginine has been demonstrated in vitro in axoplasm extruded from the giant axons of squid and in injured and regenerating vertebrate nerves. (bvsalud.org)
  • Injury-induced cytochrome c -specific cleavage of caspase-9 followed by activation of caspase-3 in mature brain correlated with marked increases in Apaf-1 and caspase-3 mRNA and protein expression. (jneurosci.org)
  • To start with, we have been interested in whether or not n4bp3 is expressed within the establishing nervous system of X. laevis and investigated its expression profile in the course of early embryogenesis by full mount in situ hybridization experiments using a specific antisense n4bp3 RNA probe. (cret-signal.com)
  • For up to 35 days after challenge, host factors including complete blood counts with T lymphocyte subsets, type I/III interferon (IFN) activity, neutralizing and total FMDV-specific antibody titers in serum, as well as antibody-secreting cells (in 6 non-vaccinated animals) were characterized in the context of viral infection dynamics. (biomedcentral.com)
  • There was a transient peak of FMDV-specific serum IgM in non-vaccinated animals after challenge, while IgM levels in vaccinated animals did not increase further. (biomedcentral.com)
  • Abnormal localization of leucine-rich repeat kinase 2 to the endosomal-lysosomal compartment in lewy body disease. (neurotree.org)
  • Nucleocytoplasmic localization of FOXO1 was determined by nucleocytoplasmic isolation and immunofluorescence. (biomedcentral.com)
  • There are numerous examples of ocular surface diseases in which the corneal-specific epithelial qualities are not maintained and significant anterior eye physiologic perturbations occur resulting in dramatic loss of vision. (molvis.org)
  • They inhibit DNA/RNA polymerization after being incorporated into nascent DNA/RNA, which prevents primer extension. (plos.org)
  • 5 ). Data from case reports, case series, cohort studies, in Cutavirus in Malignant vitro work, and animal studies indicate that the infectious Melanoma period for sexual transmission of Zika virus is consider- ably shorter than the period during which viral RNA can be detected in semen. (cdc.gov)
  • Blood vessels in the vertebrate brain are composed of a single layer of endothelial cells that possess distinct functional properties that allow the passage of necessary nutrients yet prevent unwanted entry of specific toxins and pathogens into the brain. (elifesciences.org)
  • Long non-coding RNA CASP5 promotes the malignant phenotypes of human glioblastoma multiforme. (cancerindex.org)
  • This may suggest gender-specific differences in the ability to detect distinct odorants, specifically human host-derived volatiles. (ijbs.com)
  • Their study in humans, however, has been hampered by the unavailability of inner ear RNA from human subjects, making the mouse an invaluable model for studying miRNA development and regulation in the inner ear [ 8 ]. (biomedcentral.com)
  • Localization of Parkinson's disease-associated LRRK2 in normal and pathological human brain. (neurotree.org)
  • Caricasole A, Sala C, Roncarati R, Formenti E, Terstappen GC: Cloning and characterization of the human phosphoinositide-specific phospholipase C-beta 1 (PLC beta 1). (smpdb.ca)
  • Peruzzi D, Calabrese G, Faenza I, Manzoli L, Matteucci A, Gianfrancesco F, Billi AM, Stuppia L, Palka G, Cocco L: Identification and chromosomal localisation by fluorescence in situ hybridisation of human gene of phosphoinositide-specific phospholipase C beta(1). (smpdb.ca)
  • Chromosome painting, performed on metaphase chromosomes preparations with an X chromosome specific probe (P5222-DG.5, Oncor), 17 confirmed that these hybrids contained only one X chromosome. (bmj.com)
  • [ 10 ] In addition, gene expression profiling studies support the concept that radial glial cells from different neuroanatomic sites may be predisposed to acquiring particular genetic aberrations that result in ependymomas with site-specific genetic signatures and biologic potential. (medscape.com)
  • An antibody prepared against a peptide sequence specific to the Aedes aegypti GPI-linked carbonic anhydrase labels plasma membranes of a number of cell types including neuronal cells and muscles. (silverchair.com)
  • Using a Gem-specific antibody, we show that the bulk of Gem/Ca v 1.3a immunoreactivity in HCs is restricted to basally located focal spots. (jneurosci.org)
  • Both groups had a lasting increase of specific IgG and neutralizing antibody after challenge. (biomedcentral.com)
  • We further localized NOGO-A expression during key phases of CNS development by in situ hybridization. (biomedcentral.com)
  • We examined the expression patterns of rab11a, rab11ba and rab11bb using RT-PCR and in situ hybridization. (researchsquare.com)
  • thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)
  • Together, our work identified Bonus as a regulator of tissue-specific gene expression and revealed the importance of SUMOylation as a regulator of complex formation in the context of transcriptional repression. (elifesciences.org)
  • It is well documented that increased expression of LH receptor (LHR) in granulosa cells (GC) and specific changes of intrafollicular factors such as the insulin-like growth factor (IGF) and inhibin-activin-follistatin systems play a critical role in E 2 production in the DF [ 6 , 7 ]. (biomedcentral.com)
  • FISH (fluorescence in situ hybridisation) analysis was performed essentially as described by Lichter et al 15 with minor modifications. (bmj.com)
  • Data table showing topics related to specific cancers and associated disorders. (cancerindex.org)
  • Results Single-cell RNA sequencing (scRNA-seq) data analysis on the Zeisel et al. (sagepub.com)
  • Moreover, RNAscope analysis confirmed co-localization of Glra3 with Slc17a6 or Slc32a1 in the central nervous system areas sug- gested from the single-cell data. (sagepub.com)
  • Finally, we showed that Bonus SUMOylation is mediated by the SUMO E3-ligase Su(var)2-10, revealing that although SUMOylation of TIF1 proteins is conserved between insects and mammals, both the mechanism and specific site of modification is different in the two taxa. (elifesciences.org)
  • A multiple alignment of the amino acid sequence across divergent species, identified five previously undescribed, Nogo-A specific conserved regions that may be relevant for development. (biomedcentral.com)
  • Dot blotting and dot hybridisation (4) evolved out of the filter hybridisation technique and provided the basic concept for DNA arrays. (ddw-online.com)
  • Presence and persistence of Zika virus RNA in munocompetent and HIV-positive persons in Germany. (cdc.gov)
  • Between the two membrane spanning domains is a 66 amino acid extracellular loop that acts as a ligand to a specific Nogo66 receptor (NgR) [ 10 ]. (biomedcentral.com)
  • Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)
  • Localization of phosphatidylinositol phosphate kinase IIgamma in kidney to a membrane trafficking compartment within specialized cells of the nephron. (neurotree.org)
  • In situ hybridization showed that AMH and CYP19 were detected in granulosa cells (GC) of healthy as well as atretic follicles. (biomedcentral.com)
  • 3] Taken together, these findings have led some to speculate that LCH is not a specific disease of epidermal Langerhans cells, but rather one of mononuclear phagocyte dysregulation. (medscape.com)
  • RNase A is an endonuclease that specifically degrades single-stranded RNA. (ice-hbv.org)
  • Our model also allows quantification of the cell specific factors affecting NRTI efficacy. (plos.org)
  • New insights into cell-specific and compartmental ATP and GTP production, as well as utilization and buffering strategies and their relationship with known retinal and cellular functions, are discussed. (molvis.org)