Whereas in fungal gene expression regulation fungal. Medical search. Frequent questions

Their presence in the cytoplasm is directly or indirectly detected by receptors, so-called resistance (R) proteins, or nucleotide-binding leucine-rich repeat (NB-LRR) proteins encoded by R- genes. (researchsquare.com)
The mature miRNAs are loaded onto ARGONAUTE (AGO) proteins, forming a miRNA-induced gene silencing complex (miRISC). (biomedcentral.com)
Interestingly, trapping device simplification was accompanied by expansion of gene families encoding adhesion proteins and their increasing adhesiveness on trap surfaces. (mcmaster.ca)
Several molecular mechanisms with antifungal agents have been reported for C. albicans where insertions, deletions, and point mutations in genes codifying target proteins are frequently related to the antifungal drug resistance. (intechopen.com)
Furthermore, gene overexpression is also frequently associated to antifungal resistance as well as an increase in the activity of proteins that reduce oxidative damage. (intechopen.com)

Ruderfer DM, Roberts DC, Schreiber SL, Perlstein EO, Kruglyak L. Using expression and genotype to predict drug response in yeast. (broadinstitute.org)
The yeast pheromone pathway has been extensively characterized, and is an ideal system for studying transcriptional regulation. (nih.gov)
In this work, we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter. (nih.gov)
Altogether, the results reveal the potential of the FUN-LOV optogenetic switch to control two biotechnologically relevant phenotypes such as heterologous protein expression and flocculation, paving the road for the engineering of new yeast strains for industrial applications. (nih.gov)
Here, we report a novel optogenetic switch (FUN-LOV) based on the LOV domain interaction of two blue-light photoreceptors (WC-1 and VVD) from the fungus N. crassa In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light. (nih.gov)
We used FUN-LOV to optogenetically manipulate, in yeast, two biotechnologically relevant phenotypes, heterologous protein expression and flocculation, resulting in strains with potential industrial applications. (nih.gov)
We analyse by means of quantitative PCR the expression of several stress induced genes in 24 efficient commercial wine yeast strains at the beginning of vinifications performed under standard conditions or with small variations in pH and temperature. (unboundmedicine.com)
TY - JOUR T1 - Analysis of the expression of some stress induced genes in several commercial wine yeast strains at the beginning of vinification. (unboundmedicine.com)
We analyse the effects of SVs on gene expression, quantitative traits and intrinsic reproductive isolation in the yeast Schizosaccharomyces pombe. (ox.ac.uk)
The QCR8 gene of the yeast K1uyveromyces lactis is transcriptionally regulated by the carbon source in the growth medium. (ox.ac.uk)
Inducible genes in yeast retain a "memory" of recent transcriptional activity during periods of short-term repression, allowing them to be reactivated faster when reinduced. (ox.ac.uk)
In a screen for cell cycle-regulated genes in the yeast Saccharomyces cerevisiae, we have identified a gene, EGT2, which is involved in cell separation in the G1 stage of the cell cycle. (ox.ac.uk)

Imre E. Somssich focuses on Genetics, WRKY protein domain, Transcription factor, W-box and Gene. (research.com)
His primary scientific interests are in Genetics, Gene, Arabidopsis, Transcription factor and WRKY protein domain. (research.com)

Much of this work is carried out in the species Candida albicans, the most prevalent fungal pathogen of humans. (ucmerced.edu)
We investigated the effect of extracellular prostaglandin E2 (PGE2) on the human fungal pathogen Candida albicans. (canada.ca)
The ISELs and their associated genes provide a comprehensive view of lncRNAs during fungal pathogen-plant interactions. (biomedcentral.com)
The first layer of immunity, known as pathogen-associated molecular pattern- (PAMP-) triggered immunity (PTI), is activated following recognition of the fungal pattern molecule chitin, by four lysin motif- (LysM-) containing protein (LYP) receptors, i.e., chitin elicitor binding protein (CEBiP), chitin elicitor receptor kinase (CERK1), LYP4 and LYP6 (Shimizu et al. (biomedcentral.com)
His Gene research incorporates themes from Evolutionary biology, Pathogen and Signal transduction. (research.com)
His Gene study deals with Biotechnology intersecting with Virulence, Real-time polymerase chain reaction and Pathogen. (research.com)
The opportunistic human fungal pathogen Candida glabrata is closely related to Saccharomyces cerevisiae, yet it has evolved to survive within mammalian hosts. (boku.ac.at)

We also tested the FUN-LOV switch for heterologous protein expression in Saccharomyces cerevisiae , where Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical-inducible system. (nih.gov)
Transcriptional analysis of C. albicans treated with PGE2 indicated differential expression of genes involved in alternative carbon source catabolism, and showed repression of genes encoding components of both the translational machinery and the homolog of Saccharomyces cerevisiae Mac1 regulon needed for iron uptake. (canada.ca)
A central fungal transcription factor, the best-studied example of which is Saccharomyces cerevisiae GCN4, is crucial for the response to amino acid limitation. (tamu.edu)

X-rays are a work of eggs in characteristics Wondering from Not fungal finished job results been by compounds to free ideal times related by agents optoelectronic as Candida problems, or Cryptococcus applications. (plywoodskyscraper.com)

Our analysis of metabolic regulation shows that UV activates DNA repair, aromatic amino acid and carotenoid biosynthesis and represses central carbon metabolism and the fungal-like apoptotic pathway. (biorxiv.org)
Down-regulation of Spds in the mutant altered overall nitrogen metabolism and significantly reduced fungal growth, pathogenicity, and the production of aflatoxins during maize seed infection. (usda.gov)

Concept of gene, Prokaryotes as genetic system, Prokaryotic and eukaryotic chromosomes, methods of gene isolation and identification, Split genes, overlapping genes and pseudo genes, Organization of prokaryotic and eukaryotic genes and genomes including operan, exon, intron, enhancer promoter sequences and other regulatory elements. (nipaers.com)
Gene cloning and sub-cloning strategies, chromosome walking, genetic transformation, Basis of animal cloning. (nipaers.com)
Enhancement of Genetic Molecular Expression by Mineral Ions in Microbial Cells, and its Effect at the Cellular Level. (raulcuero.com)
With the advances in BMT and gene therapy, patients now have a better likelihood of developing a functional immune system in a previously lethal genetic disease. (medscape.com)

Importantly, FUN-LOV's ability to accurately manipulate gene expression, with a high temporal dynamic range, can be exploited in the analysis of diverse biological processes in various organisms. (nih.gov)
two protein structures were solved, and insights into their regulation and biological roles were gained through gene expression analysis and enzymatic assays on complex biomass. (proteopedia.org)

They can modulate various biochemical and physiological processes, including plant response and resistance to fungal pathogens, by regulating the expression of numerous genes. (researchsquare.com)
This resistance may be acquired through gene mutation. (lookformedical.com)
2017 ). In turn, rice resistance ( R ) genes recognize avirulence effectors to activate the second layer of immunity, i.e., effector-triggered immunity (ETI), to contain the M. oryzae infection (Bialas et al. (biomedcentral.com)
The main differences seem to include an extended repertoire of adhesin genes, high drug resistance, an enhanced ability to sustain prolonged starvation and adaptations of the transcriptional wiring of key stress response genes. (boku.ac.at)
While traditional breeding techniques are often used to improve aflatoxin resistance in maize, 'Host Induced Gene Silencing' (HIGS) through RNA interference (RNAi) is being evaluated as a potential alternative. (usda.gov)
However, little is known about induced resistance nor about defense gene expression and regulation in kernels. (iita.org)
This chapter summarizes the main molecular mechanisms to C. albicans antifungal drug resistance, besides offering an overview of new antifungal agents and new antifungal targets to combat fungal infections. (intechopen.com)

His WRKY protein domain study incorporates themes from Genome, Genomics, Fungal protein and Abiotic component. (research.com)
Using genomics, transcriptomics and proteomics we have identified two fungal symbiosis factors that act synergistically in the apoplast, the enzymatically active ecto-5-nucleotidase ( Si E5NT) and nuclease A ( Si NucA). (mpg.de)

However, it is now known that the untranslated region of mRNA is involved in many regulatory aspects of gene expression in eukaryotic organisms. (wikipedia.org)
The cpc-1 mRNA contains two upstream open reading frames (uORFs) in its >700-nucleotide(nt) 5' leader, and its expression is controlled at the level of translation in response to amino acid starvation. (tamu.edu)
In all cases, high levels (with differences among strains) of GPD1 mRNA but quite low expression of other stress genes (TRX2, HSP104 and SSA3) were found. (unboundmedicine.com)
For all these genes, mRNA levels increase as temperature decreases or pH increases. (unboundmedicine.com)
The maintenance of these memory gene loops (MGLs) during intervening periods of transcriptional repression is required for faster RNA polymerase II (Pol II) recruitment to the genes upon reinduction, thereby facilitating faster mRNA accumulation. (ox.ac.uk)

Opportunistic fungal sepsis is seen most often in immunosuppressed patients with severe neutropenia or in postoperative patients with intravenous catheters and usually follows prolonged antibiotic therapy. (lookformedical.com)

The lab is also beginning to study interspecies interactions between different fungal and bacterial species. (ucmerced.edu)
Variations in transcriptional responses of CE15-encoding genes under different growth conditions suggest nonredundant functions for enzymes found in species with multiple CE15 genes and further illuminate the importance of GEs in native lignin-carbohydrate disassembly. (proteopedia.org)
TEs, associated with species-specific genes involved in disease process, also possibly had an incidence on evolution of pathogenicity by promoting translocations of effector genes to highly dynamic regions and thus tuning the regulation of effector gene expression in planta. (pasteur.fr)
PTI comprises both physical and chemical defence responses, e.g. the deposition of lignin-like compounds in the cell wall, the production of reactive oxygen species (ROS) and the activation of signalling cascades that modulate gene expression. (researchsquare.com)
We performed PCR-based sequencing of one region exhibiting introgression in 40 C. immitis isolates to confirm and better define the extent of gene flow between the species. (broadinstitute.org)

In this project, we propose to dissect plant immunity and cell death mechanisms induced by dAdo during fungal accommodation and the associated signalling pathways by genome mining and manipulation of host and fungal pathways. (mpg.de)

The bacterial GEs were able to utilize substrates lacking 4-OH methyl substitutions, known to be important for fungal enzymes. (proteopedia.org)
The gene expression analysis demonstrated that bacterial GE-encoding genes were differentially expressed as response to different carbon sources. (proteopedia.org)
Conclusions: Bacterial GEs exhibit much larger diversity than fungal counterparts. (proteopedia.org)
Clearly, interspecies bacterial communication influences the regulation of gene expression. (asmblog.org)

Streptococcal pathogens continue to evade concerted efforts to decipher clear-cut virulence mechanisms, although numerous genes have been implicated in pathogenesis. (cdc.gov)

Our results suggest that the expansion of adhesion genes in NTF genomes and consequential increase in trap surface adhesiveness are likely the key drivers of fungal adaptation in trapping nematodes, providing new insights into understanding mechanisms underlying infection and adaptation of pathogenic fungi. (mcmaster.ca)

However, only few studies have profiled fungal lncRNAs in a genome-wide manner during host infection. (biomedcentral.com)

Colonization of plant roots by the beneficial fungal endophyte Serendipita indica (syn. (mpg.de)
Fungal colonization of fiberglass insulation in the air distribution system of a multi-story office building: VOC production and possible relationship to a sick building syndrome. (cdc.gov)

His WRKY protein domain research integrates issues from MAMP, Transcriptional regulation, Immunity, Regulation of gene expression and Abiotic component. (research.com)

Fungal Genet Biol. (trichoderma.info)

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi. (nih.gov)

Deletion analysis of the KlQCR8 promoter shows that an element located between -144 bp and -113 bp specifically controls induction of QCR8 gene expression on non-fermentable carbon sources. (ox.ac.uk)

Her lab is interested in investigating how transcriptional networks underlie the regulation of gene expression during biofilm development. (ucmerced.edu)
His study explores the link between Transcription factor and topics such as Transcription that cross with problems in Reprogramming, Transcriptional Networks and Reporter gene. (research.com)

Although they are called untranslated regions, and do not form the protein-coding region of the gene, uORFs located within the 5' UTR can be translated into peptides. (wikipedia.org)
It is important to consider that mutations in 3' untranslated regions have the potential to alter the expression of several genes that may appear unrelated. (wikipedia.org)

Nucleus-specific expression in the multinuclear mushroom-forming fungus reveals different nuclear regulatory programs. (broadinstitute.org)
In our study, we identified miRNAs in hops and their expression patterns in the roots of susceptible and resistant hop cultivars in the early stages of infection with the fungus V. nonalfalfae . (researchsquare.com)
Disruption of the Spds gene in A. flavus halted growth and required exogenous supply of Spd for the fungus to grow. (usda.gov)

Here we combine computational and experimental approaches to study transcriptional regulation mediated by Ste12, the key transcription factor in the pheromone response. (nih.gov)
To identify elements other than Tup1 that are involved in regulation of the Mac1 regulon, we screened a C. albicans transcription factor mutant library with this FRE7 promoter fused to a lacZ reporter. (canada.ca)
His Transcription factor research includes elements of Arabidopsis, Mutant, Regulation of gene expression, Transcription and Plant defense against herbivory. (research.com)
His primary areas of investigation include Arabidopsis, Transcription factor, Cell biology, WRKY protein domain and Gene. (research.com)

There was little overlap between AMB- or CAS-responsive genes in biofilms and those that have been identified as AMB, FLU, or CAS responsive in C. albicans planktonic cultures. (who.int)

Analysis of the predicted functions of these associated genes suggested that lncRNAs regulate pathogenesis-related genes, including xylanases and effectors. (biomedcentral.com)

Interestingly, FLU-exposed biofilms showed no significant changes in gene expression over the course of the experiment. (who.int)
In AMB-exposed biofilms, 2.7% of the genes showed altered expression, while in CAS-exposed biofilms, 13.0% of the genes had their expression modified. (who.int)
Biofilms were stained with SYTO 9 ( green , live bacteria), FUN-1 (intracellular red , live fungal cells), calcofluor white ( blue , fungal cell wall). (asmblog.org)

Micro RNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. (researchsquare.com)
Gene regulation, RNA processing and Post transcriptional modifications. (nipaers.com)
Then, the miRISC binds to target sites with sequences complementary to the miRNAs, leading to either cleavage or translational inhibition of the target mRNAs, or methylation of the target sequences, resulting in post-transcriptional and transcriptional gene silencing, respectively. (biomedcentral.com)

CONCLUSIONS: Important levels of expression of GPD1 (but not of other stress genes) are required during the first hours of vinification, because of the need for glycerol production to counteract the hyperosmotic stress at this point. (unboundmedicine.com)

To implement effective RNAi against A. flavus requires identification of suitable gene target/s that are critical for fungal growth and aflatoxin production. (usda.gov)
The results presented here show that targeting A. flavus Spds gene through RNAi approach in future might be effective in reducing both fungal growth and aflatoxin contamination in maize. (usda.gov)
In this study, maize oligonucleotide arrays and a pair of closely-related maize lines varying in aflatoxin accumulation were used to reveal the gene expression network in imbibed mature kernels in response to A. flavus' challenge. (iita.org)

Complementation of the 'spds mutant with a wild type (WT) A. flavus Spds gene restored the WT phenotype. (usda.gov)

Notably, a sua7-1 mutant or the endogenous INO1 gene that lacks this MGL does not display such faster reinduction. (ox.ac.uk)

To date, more than 500 MIR genes have been identified in the rice genome, generating more than 700 mature miRNAs (Tang and Chu 2017 ). (biomedcentral.com)

CLR1 and CLR2 are light dependent regulators of xylanase and pectinase genes in Trichoderma reesei. (trichoderma.info)
Identification of Differentially Expressed Genes in Trichoderma koningii IABT1252 During Its Interaction with Sclerotium rolfsii. (trichoderma.info)

Analysis of the promoter of the Mac1 regulon component FRE7 revealed a well-conserved palindromic Mac1p binding site that was critical for expression of a downstream reporter. (canada.ca)
We then define a modular cloning system, including antibiotic selections, integration sites, and reporter genes, and use the transcriptomics to derive strong constitutive and regulated promoters. (biorxiv.org)
Induction of the reporter gene and protein-binding was dependent on the presence of a functional KlCAT8 gene, suggesting that, in K. lactis, K1Cat8p acts in the transcriptional regulation of respiratory function. (ox.ac.uk)

We demonstrate that this memory mechanism is associated with gene loop interactions between the promoter and 3' end of the responsive genes HXK1 and GAL1FMP27. (ox.ac.uk)

Differentially expressed miRNAs target 49 transcripts and their gene ontology enrichment analysis showed that the susceptible cultivar responds by modulating the processes of protein localization and pigment synthesis, whereas the resistant cultivar responds by modulating transcription factors and hormone signalling. (researchsquare.com)

Inactivation of Spds significantly reduced mycelial growth and sporulation in vitro and addition of exogenous Spd was required to restore fungal growth and sporulation. (usda.gov)
RefAuthors Nagarajan, U. M., Peijnenburg, A., Gobin, S. J., Boss, J. RefAuthors M., van den elsen, P. J. RefTitle Novel mutations within the RFX-B gene and partial rescue RefTitle of MHC and related genes through exogenous class II RefTitle transactivator in RFX-B-deficient cells. (lu.se)

Carbon source-dependent transcriptional regulation of the QCR8 gene in Kluyveromyces lactis. (ox.ac.uk)
The activating element contains no other known regulatory sites but two elements required for RNA holoenzyme functioning, raising the intriguing possibility of carbon source-dependent regulation by a subunit of the RNA polymerase holoenzyme in K. lactis. (ox.ac.uk)

All cells must detect and respond to changes in their environment, often through changes in gene expression. (nih.gov)

To decipher the potential roles of lncRNAs during infection, we identified 365 protein-coding genes that were associated with 214 ISELs. (biomedcentral.com)
His WRKY protein domain study integrates concerns from other disciplines, such as Regulation of gene expression, Jasmonate and Transcriptional regulation. (research.com)
The stress-activated protein kinase (SAPK) pathway plays a central role in coordinating gene expression in response to diverse environmental stress stimuli. (ox.ac.uk)

Mutations in the 3' UTR of the APP gene are related to development of cerebral amyloid angiopathy. (wikipedia.org)
SCID results from mutations in any of more than 15 known genes. (medscape.com)

Gene expression analysis revealed a consistent up-regulation of the adhesion genes during their lifestyle transition from saprophytic to nematophagous stages. (mcmaster.ca)
Analysis of the regulation of MatK gene expression. (mpg.de)

In planta dAdo induces activation of plant cell death marker genes, electrolyte leakage and 26S proteasome activity. (mpg.de)

This data not only reveals novel photobiology and metabolic regulation, it also allows derivation of constitutive and regulated gene expression parts. (biorxiv.org)

miRNAs are a class of small non-coding RNAs transcribed from miRNA genes ( MIRs ). (biomedcentral.com)

The pivotal role played by CPC1, combined with its translational regulation by uORFs and NCC utilization, underscores the emerging significance of noncanonical initiation events in controlling gene expression.IMPORTANCE There is a deepening and widening appreciation of the diverse roles of translation in controlling gene expression. (tamu.edu)

The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. (duke.edu)
His research integrates issues of Arabidopsis thaliana, Regulation of gene expression, Botany and Cell biology in his study of Arabidopsis. (research.com)
This host cell death is required for fungal accommodation and the establishment of a long-lasting beneficial interaction in barley and Arabidopsis. (mpg.de)
dAdo also triggers cell death in the liverwort Marchantia polymorpha , suggesting that this fungal-elicited cell death is conserved across plant lineages. (mpg.de)

In 1980, a study was conducted on the 3' UTR of the duplicated human alpha-globin genes. (wikipedia.org)
Since the regulation of gene expression is critical in the proper function of cells, this is an area of study that needs to be investigated further. (wikipedia.org)
In the current study we evaluated the importance of a polyamine (nitrogenous compound) biosynthetic gene, spermidine synthase (Spds) that is required to produce the polyamine spermidine (Spd), which is critical for living cells to survive. (usda.gov)

Notably, we discover a novel promoter from a hypothetical gene that has 9-fold activation upon UV exposure. (biorxiv.org)

2019 ). Some of these R genes have been widely exploited in rice production and have made major contributions to the control of rice blast. (biomedcentral.com)

Optogenetic switches permit accurate control of gene expression upon light stimulation. (nih.gov)

Photoregulation and phytochrome regulation of nuclear and chloroplastic gene expression. (nipaers.com)
Gene loops function to maintain transcriptional memory through interaction with the nuclear pore complex. (ox.ac.uk)

Immunoglobulins produced in a response to FUNGAL ANTIGENS. (lookformedical.com)
This confers a rapid and versatile gene expression response to the environment. (ox.ac.uk)
We predict that gene loop conformations enhance gene expression by facilitating rapid transcriptional response to changing environmental conditions. (ox.ac.uk)

Polyamines (PAs) are ubiquitous polycations that influence normal growth, development, and stress responses in living organisms and have been shown to play a significant role in fungal pathogenesis. (usda.gov)
Inoculated kernels were incubated 72 h via the laboratory-based Kernel Screening Assay (KSA), which highlights kernel responses to fungal challenge. (iita.org)

Anatomy4

Organisms8

Chemicals and Drugs11

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes32

Disciplines and Occupations1

Information Science4

Proteins5

Yeast12

Genetics2

Pathogen7

Saccharomyces3

Candida1

Metabolism2

Genetic4

Biological2

Resistance7

Genomics2

MRNA5

Opportunistic1

Species5

Pathways1

Bacterial4

Pathogens1

Genomes1

Genome-wide1

Colonization2

Immunity1

Biol1

Fungi1

Deletion1

Transcriptional networks2

Untranslated regions2

Fungus3

Transcription factor4

Albicans1

Effectors1

Biofilms3

Post-transcriptional3

Conclusions1

Aflatoxin3

Phenotype1

Mutant1

20171

Trichoderma2

Reporter3

Interactions1

Differentially1

Exogenous2

Carbon2

Changes in gene expression1

Protein3

Mutations2

Analysis2

Planta1

Metabolic regulation1

RNAs1

UORFs1

Cell4

Study3

Promoter1

20191

Control of gene1

Nuclear2

Response3

Responses2