Weak anion exchange chromatography resin. Medical search. Frequent questions

Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. (springer.com)
A theoretical description of analyte retention in high-performance liquid chromatography (HPLC) has been the subject of various publications. (springer.com)
Normal or reversed-phase liquid chromatography can be used for analysis. (springer.com)
In normal phase liquid chromatography (NP-LC), the stationary phase is more polar than the mobile phase. (springer.com)
Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds. (springer.com)
For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)
s-IP permeability was assayed by high-performance liquid chromatography determination in the urine of the lactulose to mannitol ratio. (hindawi.com)
During my college study, I found liquid chromatography to be a profound subject. (galaklc.com)
Low-bleed silica-based stationary phase for hydrophilic interaction liquid chromatography, Anal. (ecust.edu.cn)
Preparation of a low bleeding polar stationary phase for hydrophilic interaction liquid chromatography, Talanta, 2018, 182, 500-504. (ecust.edu.cn)
Poly(vinyl alcohol)-cationic cellulose copolymer encapsulated SiO2 stationary phase for hydrophilic interaction liquid chromatography, RSC Adv. 2017, 7, 21336-21341. (ecust.edu.cn)
A positively charged porous graphitic carbon stationary phase for hydrophilic interaction liquid chromatography, Talanta, 2017, 164, 159-163. (ecust.edu.cn)
Poly(vinyl alcohol) Modified Porous Graphitic Carbon Stationary Phase for Hydrophilic Interaction Liquid Chromatography, Anal. (ecust.edu.cn)
10. Yahui Peng, Yanjie Hou, Feifang Zhang, Guobin Shen, B. C. Yang* , A hyperbranched polyethylenimine functionalized stationary phase for hydrophilic interaction liquid chromatography, Anal Bioanal Chem. (ecust.edu.cn)
An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography. (ecust.edu.cn)
Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons. (govsci.com)

These resins are prepared preswollen although cellulose exchangers swell in a strong basic environment to increase access to binding sites. (wikipedia.org)
Like all anion exchangers, the resin carries a positive charge that interacts favorably with negative charges. (wikipedia.org)
Cellulose, dextran, agarose, and other insoluble complexes are unaffected because they compose inert matrices, hence why they are so often derivatized with strong and weak cation and anion exchangers in chromatography. (wikipedia.org)
Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules. (sartorius.com)
Weak ion-exchangers are used for the analysis of inorganic ions, a technique more specifically termed ion chromatography. (galaklc.com)

In organic acid fermentation, DuPont's acrylic chromatography resins can separate the organic acids from contaminating salts and residual dextrose with sharp and efficient elution peaks. (dupont.com)
Recent advances in pH-based elution for ion-exchange chromatography utilizing volatile buffers have enabled rapid native mass-spectrometry-based identification of PTMs and proteoforms associated with protein therapeutics. (bvsalud.org)
In this study, we introduce a methodology that predicts the elution behavior of antibodies in multimodal chromatography based on their amino acid sequences. (bvsalud.org)
Elution of the gold-loaded resins was also examined, and a shortlist of promising structural features was derived from the combined results. (edu.au)
The quinuclidine resin was subjected to four cycles of B/R gold loading and elution. (edu.au)
Full capsids have a lower pI than empty capsids (5.9 versus 6.3 on average), and this charge difference can be used in anion or cation exchange with salt elution to separate the capsids. (insights.bio)
In addition, hybrid chromatography can be used for the viruses which are difficult to combine with ion exchange chromatography, or even if combined, it is difficult to establish suitable conditions for elution. (alfa-chemistry.com)

Experience unparalleled selectivity, resolution, yield and ease-of-use for a variety of biomolecules with the unique separation properties of our multimodal chromatography resins. (bio-rad.com)
Multimodal chromatography has emerged as a promising technique for antibody purification, owing to its capacity to selectively capture and separate target molecules. (bvsalud.org)
To tackle this issue, efficient predictive tools are essential for the development and optimization of multimodal chromatography processes. (bvsalud.org)

Ion Exchange PhyTip ® columns is used for efficient parallel purification of small protein samples. (biotage.com)
Refers to chromatography columns with inner diameters less than 0.5 mm. (speciation.net)
High surface accessibility of binding sites allows capacities beyond resin-based columns for large biomolecules, as well as rapid convection-based mass transfer. (sartorius.com)
Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. (tosohbioscience.com)
The CaptureSelect TM AAVX PhyTip® columns with our proprietary Dual Flow Chromatography are compatible with most 8, 12, and 96-channel automation liquid handling robots. (biotage.com)
PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step. (biotage.com)
Our AAV PhyTip columns are packed with a high capacity AAV affinity resin, which routinely generates purified samples of his-tagged protein with 60 - 80% sample recovery. (biotage.com)
PhyTip columns process samples by dual flow chromatography which is a gentle purification process for high levels of biologically active protein. (biotage.com)
Our Protein A PhyTip columns comes with dual flow chromatography protocols that have be validated with real protein and can be used on any automated liquid handling system. (biotage.com)

There are several ways to model the separation mechanism: partition, adsorption, ion exchange, and size exclusion. (springer.com)
This review's highlight is the recovery of lactic acid by adsorption technique using ion-exchange resins with a brief focus on the potential of in-site separation strategies alongside the important factors that influenced the lactic acid recovery process by ion exchange. (springeropen.com)
Ion exchange and adsorption method is a cost-efficient separation technique that has been widely used in the pharmaceutical industry. (seplite.com)

Ca ++ Pure-HA is a hydroxyapatite resin used in the chromatographic separation of biomolecules. (selectscience.net)
Resins are available for capture, separation, polishing and analysis of wide range of biomolecules, and streamline process development from drug discovery through clinical trials and bioproduction. (bio-rad.com)
Purify affinity-tagged proteins, monoclonal antibodies, and other biomolecules with a range of affinity resins including immobilized metal affinity chromatography (IMAC), Protein A, and activated resins. (bio-rad.com)
Separate biomolecules that have weak or strong hydrophobic regions from contaminants with resins of various strengths. (bio-rad.com)
Join us on a journey through the monolith matrix to discover how convective chromatography supports the robust separation of large biomolecules. (sartorius.com)
Sartorius experts represent extensive experience in chromatography and purification of biomolecules as well as analytical methods development. (sartorius.com)
Ion-exchange is used for the analysis of small ions but the key application area of the technique is the separation of biomolecules such as peptides, proteins, and oligonucleotides. (galaklc.com)

To ensure that the resin is protonated and positively charged, the chromatography should be performed at least 2 pH units below the pKa of the amine group, 10. (wikipedia.org)
A set of 48 novel ion-exchange (1EX) resins bearing a range of amine and quaternary ammonium moieties were prepared on a polystyrene-divinylbenzene substrate. (edu.au)
A common reaction scheme was employed for most preparations, via treatment of the exhaustively chloromethylated resin substrate with excess amine in dimethylformamide. (edu.au)
Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE (diethylaminoethyl). (tosohbioscience.com)

DEAE-C is a weak anion exchanger. (wikipedia.org)
The resin is a weak exchanger because it is only partially ionized over most pH values, and an efficient separation with DEAE-C chromatography requires a specific, narrow pH range. (wikipedia.org)
The exchange capacity of an ion-exchanger is an important measure of its retentivity (typically measured in milliequivalents per gram material). (galaklc.com)
Preparation of decarboxylic-functionalized weak cation exchanger and application for simultaneous separation of alkali, alkaline earth and transition metals, Talanta, 2016, 153(1): 79-82. (ecust.edu.cn)
As an organic exchanger for high molecular weight polymers, the backbone of the ion exchange resin has a network structure with many active groups that can exchange ions in the solution, such as sulfonic acid groups and carboxyl groups. (alfa-chemistry.com)

DuPont chromatography resins quickly and efficiently elute the salts and non-anion compounds while retaining the anionic compounds. (dupont.com)
For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
This is how dual flow chromatography works - capture, wash, elute. (biotage.com)

Purify charged and polar molecules with anion and cation exchange chromatography (AEX, CEX) resins available in various particle sizes, ionic forms, and binding capacities. (bio-rad.com)
Ion-exchange phases separate solutes on the basis of differences in ionic charge. (galaklc.com)
Retention in ion-exchange chromatography is determined by the pH of the eluent, the nature and ionic strength of the buffer and temperature. (galaklc.com)
For the former, ionic species are attached to the silica surface, whereas for the latter the ion-exchange groups are distributed throughout the matrix. (galaklc.com)

Stable and reliable ion exchange processing solutions for organic and amino acid production. (dupont.com)
Lysine is usually recovered from the fermented broth of sugar molasses, but it requires a larger volume of ion exchange resin for production than any other amino acid. (dupont.com)
A cation-exchange separation was optimized using volatile buffers to provide online hyphenation for native mass spectrometry to profile modifications and proteoforms present at the native level of a biotherapeutic, such as deamidation, O-glycosylation, amino acid substitution, N-linked glycosylation and oxidation. (bvsalud.org)

The relative gold sorption capacities of these resins were compared and assessed in terms of common structural features, with a view to developing a gold-selective resin for use with thiosulfate leachates. (edu.au)
Wide pore materials will typically have lower ion-exchange capacities. (galaklc.com)

The separation and purification of viruses mainly include ultracentrifugation, chromatography, chemical reagents and ultrafiltration. (alfa-chemistry.com)

Weak acid cation macroporous resin products have carboxylic functional groups which give high chemical efficiency in many applications including the removal of bicarbonate alkalinity. (purolite.com)
SEPLITE® LSC780 Ion Exchange Chelating Resin is a unique drinking water grade, macroporous resin designed for the removal of boron from drinking water. (seplite.com)

UniPS®, UniPSN, and UniPMM resins have been mainly applied in preparative RPC purification of peptides, oligonucleotides, proteins, and natural products, while UniPS® products of small particle sizes (especially non-porous resins) have been used as analytical chromatography media. (amerigoscientific.com)

Gas chromatography (GC) coupled on-line to mass spectrometry (MS) is routinely applied for the simultaneous analysis of multiple hormones in plant tissues [ 10 ]. (biomedcentral.com)
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)

Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids. (wikipedia.org)
The proteins are released from the resin by increasing the salt concentration of the solvent or changing the pH of the solution as to change the charge on the protein. (wikipedia.org)
This exchange is utilized to separate proteins that have faintly differing charges. (wikipedia.org)
Chromatography of Proteins. (wikipedia.org)

The resin retained 89% of the initial gold sorption capacity, and 99% of the adsorbed gold was eluted with a modified nitrate eluent. (edu.au)
Online Gas-Free Electrodialytic KOH Eluent Generator for Ion Chromatography, Anal. (ecust.edu.cn)
Fabrication and evaluation of an electrodialytic carbonate eluent generator for ion chromatography, Talanta 2016, 159, 143-147. (ecust.edu.cn)

The resultant highly concentrated, pure AAV sample, containing both full and empty capsids, required a polishing step using ion exchange chromatography to reduce the empty capsids. (insights.bio)
That is then followed by an anion exchange polish to remove empty capsids. (biotage.com)

Ion exchange chromatography can be used either for a separate purification step or for a second polishing purification step after affinity chromatography purification as an orthogonal chemistry to obtain a highly pure final product. (biotage.com)
They are specially designed for high resolution and high binding capacity purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media. (biokal.com)
As a global leader in resin technology, we develop and manufacture small beads that are used in the most regulated industries in the world to separate, remove or recover very specific elements and compounds. (purolite.com)
NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)

DuPont offers a full selection of high-quality strong acid cation and weak base anion ion exchange resins to tackle the challenging processing conditions that come with synthesizing organic and amino acids. (dupont.com)
DuPont's resins offer cost-effective deashing and chromatographic purification of organic and amino acids while resisting osmotic stress. (dupont.com)
Explore a wide selection of size exclusion (or gel filtration) chromatography resins for diverse applications, including those requiring use of organic eluents. (bio-rad.com)

Concentration and buffer exchange was completed by tangential flow filtration using hollow fibers. (insights.bio)

A set of promising moieties (piperidineethanol, quinuclidine, TMEDA, and methyl derivatives of diethanolamine, imidazole, morpholine and piperazine) had similar [Au]R/Cm values to the commercial IEX resins Dowex-1, IRA-410 and Amberlite A26 (-10 mmol/mol). (edu.au)

In ion exchange chromatography, the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. (tosohbioscience.com)
For instance, in anion exchange chromatography a molecule with a pI of 6.8 is run in a mobile phase buffer at pH 8.0 with the solid support pKa at 10.3. (tosohbioscience.com)

For example, a combination of ceramic hydroxyapatite chromatography and cation exchange column. (alfa-chemistry.com)

2023. "Accumulation mechanisms for contaminants on weak-base hybrid ion exchange resins. (pnnl.gov)
2023. "Part I: Predicting Performance of Purolite A532E Resins for Remediation of Comingled Contaminants in Groundwater. (pnnl.gov)
2023. "Part II: Predicting Performance of DOWEX 21K Resin for Remediation of Comingled Contaminants in Groundwater. (pnnl.gov)

The innovative tentacle technology of Fractogel® resins offers a number of advantages compared to conventional materials. (emdmillipore.com)

We analyzed a total of 64 full-length antibodies, including IgG1, IgG4, and IgG-like multispecific formats, which were eluted using linear pH gradients from pH 9.0 to 4.0 on the anionic mixed-mode resin Capto adhere. (bvsalud.org)
We performed affinity capture chromatography with the Capto™ AVB, HiTrap™ column. (insights.bio)

Cation-exchange phases contain negatively charged functional groups and retain positively charged cations. (galaklc.com)
Conversely, anion-exchange phases retain negatively charged analytes by their positively charged functional groups. (galaklc.com)

Innovative solutions - such as monolithic chromatography - are needed to purify these new modalities. (sartorius.com)

Alfa Chemistry can perform exchange capacity analysis on ion exchange resin samples provided by customers. (alfa-chemistry.com)
Alfa Chemistry is a company with advanced resin analysis technology. (alfa-chemistry.com)

Protein A SepFast is a family of affinity chromatography media for the purification of immunoglobulins. (biokal.com)
The range of Protein A SepFast media is developed and supported for production scale chromatography use. (biokal.com)
The HiSep DEAE SepFast HighRes Plus column belongs to a group of weak anion ion-exchange chromatography media with reduced bead size (20 - 50 μm). (biokal.com)
Explore a complete line of chromatography resins and media with various base bead and ligand chemistries for your lab-scale and bioproduction purification needs. (bio-rad.com)
Reverse phase chromatography (RPC) resins are important alternatives to silica-based RPC media because of their have unique selectivity, high capacity and wide pH stability range. (amerigoscientific.com)
These high-performance chromatography media can also be used as reverse phase solid phase extraction (SPE) sorbents. (amerigoscientific.com)

Unlike in other CE modes, where the amount of sample present can be determined from the area under the peaks as in chromatography, quantitation in CITP is mainly based on the measured zone length which is proportional to the amount of sample present. (speciation.net)

From high-throughput resin screening to small-scale method development and scale-up optimization, find chromatography resins in prepacked formats for the entire purification development cycle. (bio-rad.com)
However, the optimization of chromatography parameters remains a challenge due to the intricate nature of protein-ligand interactions. (bvsalud.org)

Due to the physiological buffers used during the protocol, ion exchange chromatography is a generally gentle purification method. (biotage.com)

Size-exclusion chromatography Stationary phase Rousseau, Ronald W. (wikipedia.org)

The strength of the bond between the resin and protein is highly dependent on the pH range in the column and the pI of the protein of interest. (wikipedia.org)
The TSKgel DNA-NPR weak anion exchange column is designed to analyze nucleic acids. (selectscience.net)
Protein A SepFast HighRes has a particle size of 20 - 50 µm and is good for small affinity chromatography column run at shorter residence time (i.e. higher flowrate). (biokal.com)
Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. (biotage.com)

Perchlorate-selective ion exchange resin is a best available technology for impacted groundwater remediation. (purolite.com)
While several resin technologies can treat perchlorate, single-use perchlorate selective resins have become a standard in perchlorate treatment due to its simple operation, effectiveness of removal, and lower overall cost of treatment. (purolite.com)
Today, the most common ion exchange treatment option for perchlorate is Purolite ® A532E , a single-use perchlorate selective resin certified by the WQA to NSF/ANSI/CAN 61 standard for drinking water. (purolite.com)
In addition, testing methods such as ultrasonic treatment, flow injection ion selective electrode method, and infrared spectroscopy have also been developed to study the exchange capacity of specific ion exchange resins. (alfa-chemistry.com)

For the downstream workflow of virus production, affinity chromatography purification has been found to effectively remove impurities such as plasmid DNA and host cell RNA/DNA. (biotage.com)

SUNRESIN is providing a wide range of Ion Exchange Resins to prepare Water for Human consumption. (seplite.com)

The dissolved citric and lactic acids are subsequently purified through a deashing step using ion exchange resins. (dupont.com)

Figure 2 shows how HILIC complements other areas of chromatography and extends the range of separation options. (springer.com)
The weak-base resins prepared in this manner were then exhaustively allcylated with either benzyl chloride or iodomethane, to provide a range of structurally related moieties. (edu.au)
This innovative methodology utilizing biomass (2G-biorefinery) had generated several kinds of bioproducts such as bioethanol, bioplastics, surfactants resin, biochemicals and a range of acids like citric and lactic acid (Ramos et al. (springeropen.com)

With 40 years of manufacturing expertise and 30 years of regulatory experience, we supply leading separation, purification and extraction technologies to support chromatography and biocatalysis applications in healthcare and life sciences. (purolite.com)

Find purification resources, resin packing calculators, webinars and events to support your bioprocessing success from process development to commercialization. (bio-rad.com)

DEAE-Sepharose, DEAE-650 and DEAE-Sephadex are commonly used in chromatography. (wikipedia.org)
Ion exchange is a water treatment process commonly used for water softening or demineralization, but it also is used to remove other substances from the water in processes such as dealkalization, deionization, and disinfection. (seplite.com)

The gold and copper thiosulfate complexes, along with the thiosulfate, trithionate and tetrathionate anions were modelled using ab-initio density functional theory (DFT). (edu.au)

Anatomy12

Organisms10

Chemicals and Drugs119

Analytical, Diagnostic and Therapeutic Techniques and Equipment59

Phenomena and Processes52

Disciplines and Occupations2

Technology, Industry, Agriculture16

Information Science4

Health Care2

Liquid Chromatography18

Exchangers5

Elution7

Multimodal chromatography3

Columns9

Adsorption3

Biomolecules7

Amine4

Exchanger5

Elute3

Ionic4

Amino acid3

Capacities2

Reagents1

Macroporous resin2

Analytical1

Mass Spectrometry2

Proteins4

Eluent3

Empty capsids2

Separate4

Organic3

Filtration1

Mmol1

Molecule2

Hydroxyapatite1

Contaminants3

Advantages1

Capto2

Positively charged2

Purify1

Alfa Chemistry2

Media6

Peaks1

Optimization2

Gentle purification1

Stationary phase1

Column4

Selective4

Impurities1

SUNRESIN1

Step usi1

Range3

Applications1

Bioprocessing1

Commonly2

Functional1