• Induced pluripotent stem cells (iPSCs) hold great potential for the modelling of these diseases or as potential therapeutic agents. (nature.com)
  • Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing. (sigmaaldrich.com)
  • This article provides an extensive step-wise overview of CRISPR Cas9 protocol that can be used to perform gene editing in human induced pluripotent stem cells (iPSCs). (sigmaaldrich.com)
  • Combining our CRISPR, ZFN gene editing , and stem cell expertise, we now offer novel stem cell lines, optimized media, and innovative kits for all areas of stem cell biology, including induced pluripotent stem cells (iPSCs), neural, mesenchymal and hematopoietic stem cell culture. (sigmaaldrich.com)
  • Pluripotent stem cells, including embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). (sigmaaldrich.com)
  • The ability to expand pluripotent cells in vitro and subject them to direct differentiation to produce specific cell types is crucial to the development of cell-based therapies to replace or restore tissue that has been damaged by disease or injury. (sigmaaldrich.com)
  • Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
  • The unit will demonstrate various types of stem cells: adult, embryonic, induced pluripotent and their specialised culture methods. (manchester.ac.uk)
  • Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
  • Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
  • Dr. Ardehali uses novel transgenic mouse models, as well as mostly human pluripotent stem cells (PSC) to address his research questions. (ucla.edu)
  • Additionally, PSCs - both embryonic stem cells and induced pluripotent stem cells - provide insights into the mechanisms involved in the differentiation and specification of heart cells. (ucla.edu)
  • Advertorial: Pluripotent Stem Cell Verification: Is There an Alternative to Teratoma Assays? (genengnews.com)
  • Pluripotent stem cells have great potential for use in regenerative medicine and are an important tool for understanding developmental and disease mechanisms. (genengnews.com)
  • The defining characteristics of pluripotent stem cells, which include embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, are straightforward: their ability to self-renew and differentiate into ectoderm, mesoderm, and endoderm germ layers. (genengnews.com)
  • The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
  • Time and resources spent expanding stem cells are wasted if the resulting population is not functionally pluripotent. (genengnews.com)
  • Manually picking colonies that contain pluripotent stem cells is possible through an analysis of colony morphology. (genengnews.com)
  • The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
  • The kit contains a set of azide-free, fluorochrome-conjugated GloLIVE antibodies that recognize two positive markers and one negative marker of human pluripotent stem cells. (genengnews.com)
  • These are the pluripotent cells that possess the ability to get differentiated into all germ layers. (grandviewresearch.com)
  • Transduction of Lhx2 into induced pluripotent stem cells (iPSCs) was also effective in generating KSL cells in vitro, as well as HSC-like activities in vivo. (ebiotrade.com)
  • This led us to question whether excessive Ca V 3 channels, retained mistakenly in engineered human-induced pluripotent stem cell-derived islet (hiPSC-islet) cells, act as an obstacle to hiPSC-islet maturation. (mdpi.com)
  • Mesenchymal stem cells are multipotent but not pluripotent, which means they can differentiate into some, or "multiple," but not all tissue types. (vin.com)
  • Induced pluripotent stem (iPS) cells are a valuable resource for discovery of epigenetic changes critical to cell type-specific differentiation. (nih.gov)
  • Although iPS cells have been generated from other terminally differentiated cells, the reprogramming of normal adult human basal prostatic epithelial (E-PZ) cells to a pluripotent state has not been reported. (nih.gov)
  • These E-PZ-iPS-like cells with normal karyotype gained expression of pluripotent genes typical of iPS cells (Tra-1-81, SSEA-3, Nanog, Sox2, and Oct4) and lost gene expression characteristic of basal prostatic epithelial cells (CK5, CK14, and p63). (nih.gov)
  • These cells are pluripotent and capable of prostatic differentiation, and therefore provide a novel model for investigating epigenetic changes involved in prostate cell lineage specification. (nih.gov)
  • These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
  • Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
  • No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • Essential role of Shp2-binding sites on FRS2{alpha} for corticogenesis and for FGF2-dependent proliferation of neural progenitor cells. (okano-lab.com)
  • Enhanced proliferation of progenitor cells in the subventricular zone and limited neuronal production in the striatum and neocortex of adult macaque monkeys after global cerebral ischemia. (okano-lab.com)
  • Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture. (sigmaaldrich.com)
  • Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson's disease . (bvsalud.org)
  • Here, we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells . (bvsalud.org)
  • This paper investigates and discusses a study done on the transplantation of oligodendrocytes progenitor cells (OPCs) derived from human embryonic stem cells (hESCs) at the site of injury as it is a primary target to promote recovery in the spinal cord by remyelination of axons. (oxjournal.org)
  • Of particular interest to Dr. Ardehali is the intrinsic signaling that trigger cardiac regeneration early in life, molecular events that regulate developmental decisions instructing cardiac progenitor cells to adopt a specific cell fate, and delivery approaches of cardiovascular progenitors into an injured heart. (ucla.edu)
  • Therefore, although S7 cells are not fully equivalent to mature beta cells, their capacity for glucose-responsive insulin secretion and rapid reversal of diabetes in vivo makes them a promising alternative to pancreatic progenitor cells or cadaveric islets for the treatment of diabetes. (nih.gov)
  • Transplantation of striatal progenitor cells that reconstitute missing DARPP-32 positive medium spiny projection neurons is a major strategy under assessment and consideration for the treatment of Huntington's disease (HD). (neurodegenerationresearch.eu)
  • The greatest single issue for development of this therapy concerns the availability of a renewable source of appropriately specified donor neural progenitor cells. (neurodegenerationresearch.eu)
  • The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
  • Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
  • Transplantation of pancreatic progenitors or insulin-secreting cells derived from human embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. (nih.gov)
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • Embryonic stem cells hold considerable promise in tissue engineering and regenerative medicine as a source of tissue-specific cells. (ca.gov)
  • Here we have succeeded in inducing human iPSC to recapitulate the main steps of retinal development and to form fully laminated 3D retinal tissue by exploiting the intrinsic cues of the system to guide differentiation ( Supplementary Fig. 1 ). (nature.com)
  • Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis. (thermofisher.com)
  • Intestinal epithelial cells (IECs) have evolved to build a complex physiological barrier that separates the intestinal lumen from the underlying mucosal tissue. (nature.com)
  • These properties provide stem cells with powerful capabilities for tissue repair, replacement, and regeneration, so human stem cells are of special interest in medical research. (sigmaaldrich.com)
  • With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
  • Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
  • Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
  • They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
  • Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
  • Such a view of early endocrine development is seen in the developmental processes of stem cells, adult mesenchymal stem cells and early endocrine cells, all of which are involved in regulating cell proliferation and differentiation within their tissue compartments. (medicalexaminationhelp.com)
  • Mammalian heart development involves differentiation of diverse cell types, including atrial and ventricular muscle cells known as cardiomyocytes (CMs), specific myocytes constituting conducting tissue such as Purkinje fibers, smooth muscle (SM) cells, and non-muscle cells including endothelial cells (ECs) and neuronal cells. (researchsquare.com)
  • Controlled differentiation that can be achieved in tissue committed cells through the co-culturing with basic fibroblast growth factor is expected to induce growth. (grandviewresearch.com)
  • and 2) bioreactor-based directed differentiation of ES cells under perfused 3D tissue-density conditions. (grantome.com)
  • Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
  • This series covers biophysical regulation of stem cell fate and function in the context of tissue development. (biomedcentral.com)
  • Advances in the fields of stem cell biology, biomaterials, and tissue engineering over the last decades have brought the possibility of constructing tissue substitutes with a broad range of applications in reg. (biomedcentral.com)
  • In this regard, tissue engineers have sought to leverage native mechanotransduction pathways to enhance in vitro stem cell. (biomedcentral.com)
  • Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. (biomedcentral.com)
  • Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
  • For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
  • Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
  • However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
  • Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
  • Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • The first steps toward a new cell culture system for human norovirus included the detection of human norovirus antigen in duodenal and jejunal enterocytes in tissue sections from human norovirus-infected transplant patients ( 11 ) and the development of human intestinal enteroids (HIEs) derived from nontransformed small intestine and colonic tissues ( 12 ). (cdc.gov)
  • The HIEs, or "mini-guts," are generated from stem cells present in the intestinal crypts isolated from human intestinal tissue and cultured indefinitely as ex vivo, 3-dimensional (3D) cultures in growth-factor-enriched media ( 13 - 15 ). (cdc.gov)
  • Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
  • of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
  • Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. (bvsalud.org)
  • Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. (bvsalud.org)
  • What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
  • Cardiomyocytes are the chief cell type in the heart and their coordinated contraction as a mass is responsible for the pumping of blood around the developing embryo. (mechanobio.info)
  • In vivo studies in an experimental model of acute myocardial infarction showed extensive survival of SiPS and SiPS-derived cardiomyocytes in mouse heart after transplantation. (elsevierpure.com)
  • Our results from 4-week studies in DMEM without cells (group 1), SMs (group-2), SiPS (group-3), and SiPS-derived cardiomyocytes (group 4) showed extensive myogenic integration of the transplanted cells in group 4 with attenuated infarct size and improved cardiac function without tumorgenesis. (elsevierpure.com)
  • ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes. (sigmaaldrich.com)
  • and, using novel in vitro and in vivo models to identify pure populations of cardiovascular progenitors capable of engraftment into the host myocardium. (ucla.edu)
  • Nevertheless, S7 cells rapidly reversed diabetes in mice within 40 days, roughly four times faster than pancreatic progenitors. (nih.gov)
  • Currently, directed differentiation of human embryonic stem cells (hESCs) to neural progenitors has the greatest potential of meeting this requirement. (neurodegenerationresearch.eu)
  • Critically we have now developed protocols for neural differentiation under defined conditions to generate progenitors that express markers of forebrain fate determination, and that differentiate to DARPP-32 positive neurons both in vitro and in vivo in animal models of HD. (neurodegenerationresearch.eu)
  • Objective: To reprogram SMs to pluripotency and show that reprogrammed SMs (SiPS) express embryonic gene and microRNA profiles and that transplantation of predifferentiated cardiac progenitors reduce tumor formation. (elsevierpure.com)
  • In our recent study, we examined the impact of the three-dimensional co-culture of FPMSCs on human embryonic stem cell-derived pancreatic progenitors' development. (biomedcentral.com)
  • Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
  • Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)
  • In the future, this cell culture platform will be further enhanced to enable efficient conversion of mouse and human ESCs to hepatocytes. (ca.gov)
  • Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
  • Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
  • Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
  • What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
  • This protocol introduces how to generate heart organoids from mouse embryonic stem cell-derived embryoid bodies (EB) in the presence of the laminin-entactin (LN/ET) complex and fibroblast growth factor 4 (FGF4) by three consecutive steps, 1) the culture of ESCs, 2) in vitro differentiation of ESCs into EBs and 3) in vitro culture of EBs for the generation of heart organoids. (researchsquare.com)
  • For 3D culture methods using embryonic stem cells (ESCs) or adult stem cells, ECMs including Matrigel and laminin were widely used for in vitro organogenesis,including the development of the brain 1 , stomach 2 , kidney 3 , and intestinal tissues 4 . (researchsquare.com)
  • Ino80 KO ESCs (Embryonic stem cells) were viable when maintained at ground state pluripotency but fail to differentiate in vitro and in vivo . (vcu.edu)
  • Identification of genes involved in in vitro differentiation induction of embryonic stem cells (ESCs) into hematopoietic stem cells (HSCs) has been challenged during last decade. (ebiotrade.com)
  • Here, we show that HSC-like cells were efficiently induced from mouse ESCs by enforced expression of Lhx2, a LIM-homeobox transcription factor. (ebiotrade.com)
  • For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
  • Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
  • Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
  • In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
  • Our findings reveal that during EHT, metabolism is a major regulator of primitive versus definitive hematopoietic differentiation. (lu.se)
  • Furthermore, transplantation of Lhx2-transduced hematopoietic cells into lethally irradiated mice resulted in multi-lineage repopulation of hematopoietic cells over 4 months. (ebiotrade.com)
  • These results demonstrate that ectopic expression of Lhx2 confers an in vivo engrafting capacity to ESC/iPSC-derived hematopoietic cells and in vivo behavior of iPSC-derived hematopoietic cells is almost identical to that of ESC-derived cells. (ebiotrade.com)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals. (sigmaaldrich.com)
  • Embryonic stem cells have the ability to differentiate into more cell types than adult stem cells. (sigmaaldrich.com)
  • They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
  • Stem cells differentiate into bone-forming osteoblasts, and mechanical stimulation is involved in this p. (biomedcentral.com)
  • A stem cell is a generic term referring to any unspecialized cell that is capable of long-term self-renewal through cell division but that can be induced to differentiate into a specialized, functional cell. (vin.com)
  • Most importantly stem cells possess the ability to differentiate into cell types of multiple lineages including adipocytes, chrondrocytes, and osteocytes. (vin.com)
  • 4,6,7 It has been proposed that some of these MSC may actually differentiate into functional renal tubular epithelial cells, though this theory remains controversial. (vin.com)
  • Androgen induced expression of androgen receptor (AR), and co-culture with rat urogenital sinus further induced expression of prostate-specific antigen, a hallmark of secretory cells, suggesting that E-PZ-iPS-like cells have the capacity to differentiate into prostatic basal and secretory epithelial cells. (nih.gov)
  • However, realizing this promise requires novel methods for guiding lineage-specific differentiation of stem cells. (ca.gov)
  • pTUNAR deficiency in mouse embryonic stem cells improves their differentiation potential towards neural lineage both in vitro and in vivo. (gencat.cat)
  • Increase of bone marrow-derived secretory lineage epithelial cells during regeneration in the human intestine. (okano-lab.com)
  • Deletion of Jarid2 leads to impaired orchestration of gene expression during cell lineage commitment. (sdbonline.org)
  • Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
  • A side-by-side comparison was made to evaluate the effect of each serum on proliferation rate, cell cycle, senescence, multi-lineage differentiation capacity, immunophenotype, and tumorigenesis of FPMSCs. (biomedcentral.com)
  • Flow cytometry analysis and three-lineage differentiation ability demonstrated that fibroblast-like cells obtained from primary culture had MSCs' characteristics. (biomedcentral.com)
  • To transfer to the Sykiotis lab the 3D follicle model established by the Costagliola lab in order to perform cell-based studies on the role of stress response pathways on iodine metabolism. (unil.ch)
  • Using novel stem cell, reprogramming and genome editing technologies we are studying how genetic pathways involved in aging and neurodegenerative disease affect formation and function of neural cells. (lu.se)
  • The pathways involved in cellular differentiation or transformation are Smad, Rho proteins, and PI3-kinase. (molvis.org)
  • Growth checkpoint pathways and their circuits were also involved in controlling proliferation and differentiation of embryonic stem cells in vitro or in vivo. (medicalexaminationhelp.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • DNA methylation profiling identified key pathways and regulators of prostatic differentiation including Wnt5a, BPM7, PTEN, and NKx3.1 using E-PZ-iPS-lke cells. (nih.gov)
  • Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • The successful generation of beta cells in vitro and regeneration of pancreatic beta cells in vivo will require a thorough understanding of the pathways that direct the normal development and function of these cells. (lu.se)
  • However, it is still unclear to what extent hiPSC may be capable of recapitulating in vitro the cellular and molecular features of the native retina, especially regarding photoreceptor differentiation and functional maturation. (nature.com)
  • Ca V 3 channels are ontogenetically downregulated with the maturation of certain electrically excitable cells, including pancreatic β cells. (mdpi.com)
  • We addressed this question by using the anterior chamber of the eye (ACE) of immunodeficient mice as a site for recapitulation of in vivo hiPSC-islet maturation in combination with intravitreal drug infusion, intravital microimaging, measurements of cytoplasmic-free Ca 2+ concentration ([Ca 2+ ] i ) and patch clamp analysis. (mdpi.com)
  • To understand the molecular and cellular mechanisms that control beta cell maturation and function in the embryonic and adult pancreas. (lu.se)
  • In vivo PET measurements with [11C]PE2I to evaluate fetal mesencephalic transplantation to unilateral 6-OHDA-lesioned rats. (okano-lab.com)
  • Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
  • The high proliferation and differentiation capacity of fetal MSCs and the role of fetal pancreatic-derived MSCs (FPMSCs) in islet generation make them good candidates for diabetes treatment. (biomedcentral.com)
  • The Wnt signaling pathway is crucial for brain development processes, which include neuron proliferation and differentiation as well as neuroblast migration and axon guidance. (wikipedia.org)
  • These questions remained until our interest was extended in the first part of our efforts to discover the molecular mechanisms to which these hormone-regulating mechanisms act to regulate proliferation and differentiation. (medicalexaminationhelp.com)
  • It was noted that until recently, growth controllers have served as a paradigm in which the regulation of pregnancy was the key element in pregnancy and the endocrine system as the source of the cellular machinery to support cell proliferation and differentiation. (medicalexaminationhelp.com)
  • Because such cells are in a perpetual cycle or are proliferating on the entire cell surface of the cell cycle, since these cells are capable of proliferation and differentiation, it is not surprising that the initial findings that such processes continue to be observed may be the result of the endocrine system\'s role in regulating proliferation and differentiation within the cells or in particular the endocrine cells. (medicalexaminationhelp.com)
  • Mesenchymal stem cells (MSCs) have received more attention due to their proliferation and differentiation capacity and low immunogenicity. (biomedcentral.com)
  • We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. (nih.gov)
  • These analyses revealed that mESCs co-cultured with HepG2 cells showed a decrease in pluripotency gene expression concomitant with up-regulation of endodermal genes. (ca.gov)
  • To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
  • To address these difficulties, R&D Systems has developed new tools that aid in the verification of stem cell pluripotency during derivation, maintenance, and expansion. (genengnews.com)
  • Moreover, colony morphology is not truly indicative of pluripotency, since cells that are not fully reprogrammed can have similar morphology. (genengnews.com)
  • Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
  • Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
  • Another assay used to verify functional pluripotency is in vitro embryoid body formation. (genengnews.com)
  • We hypothesize that undifferentiated mES cells can be expanded and maintained in the perfused 3D environment provided by our bioreactor, and that within this 3D culture model mES cell pluripotency can be maintained by culturing mES cells and fibroblast feeder cells in two separate bioreactors perfused within one circuit (compartmentalized co-culture). (grantome.com)
  • SiPS were positive for alkaline phosphatase, expressed SSEA1, and displayed a panel of embryonic stem (ES) cell-specific pluripotency markers. (elsevierpure.com)
  • E-PZ-iPS-like cells demonstrated pluripotency by differentiating into ectodermal, mesodermal, and endodermal cells in vitro, although lack of teratoma formation in vivo and incomplete demethylation of pluripotency genes suggested only partial reprogramming. (nih.gov)
  • Cell Transplantation 14: 673-682, 2005. (okano-lab.com)
  • Cell Transplantation 14: 655-663, 2005. (okano-lab.com)
  • Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances their migration and axonal regeneration after rat spinal cord injury. (okano-lab.com)
  • In vivo imaging of engrafted neural stem cells: its application inevaluating the optimal timing of transplantation for spinal cord injury. (okano-lab.com)
  • However, transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche. (bvsalud.org)
  • Transplantation of these cell lines into immune-competent mice revealed that BPTF KD enhances NK cell antitumor activity. (vcu.edu)
  • This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
  • We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
  • The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
  • Therefore, a central goal of diabetes research is to regenerate pancreatic beta cells or produce large numbers of functional beta cells for transplantation into diabetic patients. (lu.se)
  • Collagen I is used in both 2D and 3D cell culture to make the in vitro cellular environment more in vivo -like. (thermofisher.com)
  • Therefore, our aims are to develop cellular models of human neuronal aging and degeneration, to investigate underlying mechanisms responsible for dysfunction in neurons and neural stem cells in the aging and diseased brain and to dissect the regulation of normal neurogenesis. (lu.se)
  • These protocols, such as organoid cell culture methods, have provided more predictive in vitro cellular "Disease-in-a-Dish" models. (sigmaaldrich.com)
  • The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)
  • Before and during early gastrulation, the epiblast layer assumes a tightly packed cellular morphology due to an increase in E-cadherin mediated mechanical linkages between cells. (mechanobio.info)
  • Additionally, the interwoven fibers provide a scaffold allowing the cells to form 3D structures where the size of cellular aggregates is limited by the spacing between the hollow fibers. (grantome.com)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
  • In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
  • not unique for the embryonic system but are shared by other ogenesis regulation must consider different scales of multi-cellular modeling areas. (lu.se)
  • A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
  • Conversely, pTUNAR overexpression impairs neuronal differentiation by reduced neurite formation in different model systems. (gencat.cat)
  • However, to fulfill this promise, a remaining challenge is to induce human iPSC to recreate in vitro key structural and functional features of the native retina, in particular the presence of photoreceptors with outer-segment discs and light sensitivity. (nature.com)
  • We hypothesize that ovarian cancer cells will induce human ovarian TVM expression. (nih.gov)
  • I cover mainly cell biology and physiology. (nottingham.ac.uk)
  • We also have a strong interest in basic stem cell biology with a clear focus on neural stem cells and neurogenesis in the adult and aging brain. (lu.se)
  • The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
  • Understanding how an epigenetic regulator such as ATP-dependent chromatin-remodeling complexes modulate processes such as development and/or immune response is essential for our comprehension of cell biology. (vcu.edu)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • In this study, we developed a micropatterned co-culture platform for stimulating hepatic differentiation of mouse embryonic stem cells (mESCs). (ca.gov)
  • One is a method for in vitro differentiation of thyrocytes and 3D culture of follicles starting from mouse embryonic stem cells. (unil.ch)
  • Moreover, it has recently been shown that mouse and human ES cells can develop into a three-dimentional (3D) optic cup in culture that remarkably resembles the embryonic vertebrate eye 20 , 21 . (nature.com)
  • We have therefore generated `knock-in' mouse embryonic stem (ES) cells to investigate the effects of expressing a commonly found hot-spot p53 mutant, R246S - the mouse equivalent of human R249S, which is associated with hepatocellular carcinomas. (silverchair.com)
  • Correlation between Musashi-1 and c-hairy-1 expression and cell proliferation activity in the developing intestine and stomach of both chicken and mouse. (okano-lab.com)
  • In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
  • The teratoma assay is also inherently variable, depending on the site of cell inoculation and the mouse strain used. (genengnews.com)
  • The mouse blastocyst-derived embryonic stem cell (ES cell) line BLC6 efficiently differentiates into myosin heavy chain-, desmin- and myogenin- positive skeletal muscle cells when cultivated in embryo-like aggregates (embryoid bodies). (uni-luebeck.de)
  • myogenin, myoD, and myf6 are expressed in these embryoid bodies in a characteristic temporal pattern which precisely reflects the sequence observed during mouse development in vivo. (uni-luebeck.de)
  • physiologically based animals because a mouse embryonic stem cell line is used. (cdc.gov)
  • Generation of functional heart organoids from mouse embryonic stem cells. (researchsquare.com)
  • For instance, in mouse embryos this occurs as early as day 6.5 post fertilization and involves the development of a population of cardiac precursor cells in the lateral posterior epiblast just prior to formation of the primitive streak - a structure which marks the beginning of gastrulation. (mechanobio.info)
  • mouse breast cancer cell lines. (vcu.edu)
  • The objective of this 2-year project is to take the first step toward applying our bioreactor technology to ES cell research, by expanding and maintaining undifferentiated mouse embryonic stem (mES) cells in laboratory-scale versions of our bioreactor. (grantome.com)
  • 2004). In vivo and in vitro differentiation of male germ cells in the mouse. (edu.au)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • Ablation of DNMT3A in Caco-2 cells results in global DNA hypomethylation, which is linked to impaired regenerative capacity, transepithelial resistance and intercellular junction formation. (nature.com)
  • Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
  • Applicability for inducing differentiation into a wide range of tissues is attributive to the largest share of regenerative medicine. (grandviewresearch.com)
  • The differentiated cells are further used for therapeutic applications in regenerative medicine. (grandviewresearch.com)
  • They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)
  • It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. (wikipedia.org)
  • The other is the use of a new zebrafish model to investigate in vivo thyroid development in a non-invasive manner. (unil.ch)
  • Here we report that hiPSC can, in a highly autonomous manner, recapitulate spatiotemporally each of the main steps of retinal development observed in vivo and form three-dimensional retinal cups that contain all major retinal cell types arranged in their proper layers. (nature.com)
  • Dynamics of male canine germ cell development [6] "Primordial germ cells (PGCs) are precursors of gametes that can generate new individuals throughout life in both males and females. (edu.au)
  • Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
  • During embryonic development, hematopoiesis occurs through primitive and definitive waves, giving rise to distinct blood lineages. (lu.se)
  • Thus, the endocrine system can play a significant role at certain critical developmental, physiological and physiological circumstances to provide information regarding the endocrine cells that affect fertility, fertility, and development.How does the endocrine system regulate growth and development? (medicalexaminationhelp.com)
  • Our results suggest that ES cells recapitulate closely the early steps of muscle development in vivo and may serve as an excellent in vitro system to study this process. (uni-luebeck.de)
  • In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
  • In the earliest stages of development, the physical properties of the microenvironment can direct cell differentiation, and initiate the coordinated movement of groups of cells to establish the patterns that will define how the body is arranged. (mechanobio.info)
  • Cardiac specification occurs very early on during embryonic development. (mechanobio.info)
  • During development, the nuclear translocation of β-catenin, a transcriptional cofactor in the Wnt signaling pathway, is controlled by the extent of cell-cell contacts formed between cells. (mechanobio.info)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • This view is supported by key advances in basic research including, (i) progress in developing methodology for the derivation and propagation of hESC lines, and (ii) understanding of underlying mechanisms of forebrain development and neuronal sub-type specification that provide a rationale for the development of directed differentiation protocols. (neurodegenerationresearch.eu)
  • Variation in the policies across the globe is expected to impact the global human embryonic stem cell market development. (grandviewresearch.com)
  • Significant involvement of the Japanese government in the development of cell line based therapies is a significant factor and is expected to provide lucrative growth avenues. (grandviewresearch.com)
  • Embryonic stem (ES) cell research and scale-up for development of possible clinical therapies is limited by the existing 2D dish culture methods. (grantome.com)
  • Mechanical factors play a crucial role in the development of articular cartilage in vivo . (biomedcentral.com)
  • Invariant left-right (LR) patterning or chirality is critical for embryonic development. (biomedcentral.com)
  • The ability to understand and regulate human vasculature development and differentiation has the potential to benefit patients suffering from a variety of ailments, including cardiovascular disease, peripheral. (biomedcentral.com)
  • Understanding the mechanisms underlying embryonic beta cell development will be a prerequisite for the development of a universally available cell replacement therapy in diabetes. (lu.se)
  • Stage (S) 7 cells expressed key markers of mature pancreatic beta cells, including MAFA, and displayed glucose-stimulated insulin secretion similar to that of human islets during static incubations in vitro. (nih.gov)
  • NK-92 activity is reduced by the addition of heparin to the culture medium, further indicating the involvement of NKp30 (in human) and NCR1 (in mice) in killing of tumor cells. (vcu.edu)
  • A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)
  • We offer a large collection of cell culture media, supplements, bioactive small molecules, and growth factors used to control the cell fate of human iPSCs. (sigmaaldrich.com)
  • The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)
  • In conclusion, micropatterned co-cultures of mESCs and hepatic cells showed a significant promise in driving stem cell differentiation towards hepatic phenotype. (ca.gov)
  • Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
  • They proliferate easily in culture and can be cryopreserved without loss of phenotype or differentiation potential. (vin.com)
  • This kit, used in combination with the analysis of colony morphology, promotes the selection of high-quality, undifferentiated stem cell colonies, thereby reducing wasted time. (genengnews.com)
  • These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion , effectively maintaining axonal integrity in vitro . (bvsalud.org)
  • First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
  • Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson's disease . (bvsalud.org)
  • Accelerated uptake of ESC based cell therapy on in vivo and in vitro principle is expected to fuel growth through to 2024. (grandviewresearch.com)
  • An article collection in Stem Cell Research & Therapy . (biomedcentral.com)
  • Stem cell therapy in particular is an innovative new field of scientific investigation and clinical application that holds promise for a variety of diseases in veterinary medicine as well as human medicine. (vin.com)
  • In addition, it is also important to keep the immune modulatory properties of MSC in mind when interpreting the results of many stem cell therapy studies. (vin.com)
  • A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
  • Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
  • The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
  • Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
  • One such idea is cell replacement therapy (CRT). (lu.se)
  • Beta cell replacement therapy is the only available cure for type 1 diabetes. (lu.se)
  • Use of TH-EGFP transgenic mice as a source of identified dopaminergic neurons for physiological studies in postnatal cell culture. (okano-lab.com)
  • The myocytes also express functional nicotinic cholinoceptors and exhibit T-type Ca 2+ currents and later L-type Ca 2+ currents, demonstrating physiological properties of skeletal muscle cells. (uni-luebeck.de)
  • Particularly, pathological and toxicology modeling can be performed by the provision of physiological models for different human cell types. (grandviewresearch.com)
  • Outlining the physiological functions, pathological implications, and clinical applications of embryonic germline stem cells. (chamc.co.kr)
  • Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs [3] "Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. (edu.au)
  • Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation. (sigmaaldrich.com)
  • In addition to our expansive portfolio of assay-ready stem cells, serum-free cell culture media, and 3D culture solutions, we offer custom engineered stem cell lines through our easy-to-use Cell Design Studio. (sigmaaldrich.com)
  • These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)
  • showed that the differentiation assay of benchmark dose. (cdc.gov)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • A thin coating of diluted collagen solution supports cell attachment and growth of some primary cells, including hepatocytes. (thermofisher.com)
  • Pro-adhesive and chemotactic activities of thrombospondin-1 for breast carcinoma cells are mediated by alpha3beta1 integrin and regulated by insulin-like growth factor-1 and CD98. (thermofisher.com)
  • Cells were expanded in hESC growth media [23] and differentiated according to published protocols [18, 24, 25]. (oxjournal.org)
  • END-2 cells essentially prevent skeletal muscle differentiation, whereas basic fibroblast growth factor, transforming growth factor-β, and WEHI-3 cells have no or an attenuating effect, respectively. (uni-luebeck.de)
  • In addition to normal karyotypes and anchorage-dependent growth, in vivo tumor formation was not seen. (biomedcentral.com)
  • Tumor vascular cells are critical for the growth of tumor stem cells, which reside within the vascular niche. (nih.gov)
  • One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
  • We hypothesize that the ESC ovarian tumor model, with human vascular cells, will provide an ideal microenvironment to support human stem cell growth. (nih.gov)
  • Rapid advancements in this vertical include protocols for directed differentiation, defined culture systems, demonstration of applications in drug screening, the establishment of various disease models, and evaluation of therapeutic potential in treating incurable diseases. (grandviewresearch.com)
  • In vivo studies have been conducted to evaluate the therapeutic effect of FPMSCs in diabetes. (biomedcentral.com)
  • Additional characterization using single-cell imaging and dynamic glucose stimulation assays revealed similarities but also notable differences between S7 insulin-secreting cells and primary human beta cells. (nih.gov)
  • Additionally cell surface marker characterization via flow cytometry differentiates them from hematopoetic cells, though no truly unique MSC molecule has been identified. (vin.com)
  • Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. (bvsalud.org)
  • We propose that the well-controlled and versatile culture environment provided by our bioreactor is ideal for both large-scale expansion of undifferentiated ES cells and directed differentiation of ES cells using numerous strategies, including controlled exposure of the cells to molecular reagents and compartmentalized co-culture with mature cells. (grantome.com)
  • Molecular motors lie at the heart of biological processes from DNA replication to cell migration. (nih.gov)
  • Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation. (sigmaaldrich.com)
  • Also, these heart organoids exhibited the ultrastructural, histochemical and gene expression profile similarities to embryonic hearts via consecutive morphological changes like self-organization and, therefore, without the requirement of complex differentiation protocols. (researchsquare.com)
  • This translational project aims to expedite the ultimate goal of bringing stem cell therapies to clinical trial by adapting, and further developing, protocols to generate cells with markedly improved yields and a level of reproducibility and quality control that meet regulatory standards of good manufacturing practice (GMP), while maintaining a high functional efficacy in vivo in animal models of HD. (neurodegenerationresearch.eu)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • To use this knowledge to improve beta cell differentiation protocols. (lu.se)
  • Independently of the gestational periods, the qualitative evaluation showed the initial wash-in phase from the first appearance of the uterine artery to the rapid distribution in embryonic vesicles or placenta to the progressive washout, whilst there was no enhancement of either embryos or fetuses in any bitch. (edu.au)
  • Stem cells exist both in embryos and adult cells. (articlecity.com)
  • For instance, Science University and Oregon Health are engaged in developing blastocyst staged embryos of around 150 cells in order to provide hESC source. (grandviewresearch.com)
  • Information about mesenchyme, specifically mesenchymal stem cell procotols. (sigmaaldrich.com)
  • For most studies, the adult stem cell in question is actually a mesenchymal stem cell or mesenchymal stromal cell. (vin.com)
  • 11. Kramer PR, Nares S, Kramer SF, Grogan D, Kaiser M. Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro. (bvsalud.org)
  • Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems. (thermofisher.com)
  • Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
  • The knock-in cells contain higher levels of p53 that localizes to the nucleus even in the absence of genotoxic stress and yet remains non-functional, reminiscent of mutant p53 found in human tumours. (silverchair.com)
  • The generated heart organoids possess structural and functional capacity similar to atrial and ventricular parts of in vivo embryonic heart. (researchsquare.com)
  • DKK1 is one of the most upregulated genes in androgen-potentiated balding, with DKK-1 messenger RNA upregulated a few hours after DHT treatment of hair follicles at the dermal papilla in vitro. (wikipedia.org)
  • Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
  • DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)
  • The discovery of murine norovirus (MNV) in 2003 and the fact that this virus successfully replicated in a murine macrophage cell line in vitro and in primary immune cells in vivo suggested that immune cells may also support replication of human norovirus ( 7 ). (cdc.gov)
  • Stem cells reside within most tissues throughout the lifetimes of mammalian organisms. (biomedcentral.com)
  • Collagen I is the most common fibrillar collagen for cell culture and is found in skin, bone, tendons, and other connective tissues. (thermofisher.com)
  • Interestingly, bundles of aligned and uniformly spaced collagen fibrils were more prevalent in keratocytes in Y-27632-treated corneas, which is reminiscent of fibripositor-like structures that have been proposed as a mechanism of matrix deposition in embryonic connective tissues. (molvis.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • This innovative treatment involving human stem cells is yet to be tested on humans, but the testing conducted on rats has produced positive results. (oxjournal.org)
  • Genetic deficiency induces embryonic lethality in Dnmt1 and Dnmt3b-deleted mice and postnatal lethality in Dnmt3a-knockout mice at ~4 weeks of age 10 . (nature.com)
  • However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
  • In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
  • All research areas are substantiated by analyses (Figure D) monitoring the acute radiation-induced cytogenetic damage and the long-term genetic stability of cells. (gsi.de)
  • This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
  • In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
  • hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)