AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaInformation ScienceNamed GroupsHealth CareGeographicals
Hematopoietic Stem Cell TransplantationTransplantation, HomologousStem Cell TransplantationGraft vs Host DiseaseTransplantation ConditioningLymphocyte DepletionHematologic NeoplasmsTransplantation, AutologousPeripheral Blood Stem Cell TransplantationTransplantation ChimeraT-LymphocytesStem CellsBone Marrow TransplantationGraft vs Leukemia EffectTreatment OutcomeMyeloablative AgonistsTissue DonorsBusulfanCD4-Positive T-LymphocytesRemission InductionLymphocyte ActivationRecurrenceVidarabineGraft vs Tumor EffectGraft SurvivalHistocompatibility TestingImmunosuppressive AgentsLymphocyte TransfusionAntilymphocyte SerumLeukemia, Myeloid, AcuteMice, Inbred C57BLLeukemiaMultiple MyelomaHematopoietic Stem CellsRetrospective StudiesCytomegalovirus InfectionsSiblingsMinor Histocompatibility AntigensCombined Modality TherapyCord Blood Stem Cell TransplantationLeukemia, Myelogenous, Chronic, BCR-ABL PositiveWhole-Body IrradiationSalvage TherapyDisease-Free SurvivalMice, Inbred BALB CLiver TransplantationSurvival AnalysisMyelodysplastic SyndromesVirus ActivationSurvival RateNeoplasm, ResidualHistocompatibilityPrecursor Cell Lymphoblastic Leukemia-LymphomaTime FactorsCD8-Positive T-LymphocytesMelphalanT-Lymphocyte SubsetsChimerismReceptors, Antigen, T-CellTransplantation ImmunologyHepatic Veno-Occlusive DiseaseAntigens, CD34CyclophosphamideAntibodies, MonoclonalMice, TransgenicFlow CytometryCytomegalovirusHematopoietic Stem Cell MobilizationMesenchymal Stem Cell TransplantationAntibodies, NeoplasmAntineoplastic Combined Chemotherapy ProtocolsImmunosuppressionAcute DiseaseLymphocyte CountCell TransplantationHLA AntigensPrimary MyelofibrosisAnemia, AplasticFollow-Up StudiesKidney TransplantationEmbryonic Stem CellsHematologic DiseasesLeukemia, Myeloid, Accelerated PhaseGraft RejectionPrognosisLymphoproliferative DisordersAntibodies, Monoclonal, Murine-DerivedT-Lymphocytes, RegulatoryRoseolovirus InfectionsAntineoplastic AgentsCell DifferentiationCytarabineBenzamidesAdult Stem CellsImmunocompromised HostLeukemia, MyeloidAntigens, CDImmunotherapy, AdoptiveFusion Proteins, bcr-ablReceptors, Purinergic P2X5
HSCTGVHDHematopoietic stemAcute myeloidPeripheralMarrow TransplantationGraftHaematopoieticDonorsBoneMesenchymal stemDonorOrgan TransplantationHaploidenticalTransplantMalignanciesImmunotherapyRelapseMultiple MyelomaVitroAplastic AnemiaRejectionUmbilicalRefractoryRegulatory T celMonoclonal antibodiesTregsLymphocytesImmunologyDifferentiationInfusion2022ClinicalProliferationChemotherapyRemissionPatientsResponsesReceptorCytokineAdoptiveTherapiesLeukemiaCurative treatmentRenalHumansPatient'sMyeloma cellsTreatmentProgenitor cellsHuman-murineMSCsImmune systemRecipients
HSCT20
  • In contrast, in-vivo TCD is performed using anti-T cell antibodies or, most recently, post-HSCT cyclophosphamide. (wikipedia.org)
  • Experiments show that transplantation of other types of veto cells along with megadose haploidentical HSCT allows to reduce the toxicity of the conditioning regimen, which makes this treatment much safer and more applicable to many diseases. (wikipedia.org)
  • Invasive fungal disease (IFD) is a significant cause of morbidity and mortality in patients undergoing allogeneic haematopoietic stem cell transplantation (allo-HSCT). (kcl.ac.uk)
  • Allogeneic hematopoietic stem-cell transplantation (HSCT) may provide cure in this scenario. (unisr.it)
  • Hematopoietic stem cell transplantation (HSCT) results in the alteration of several components of the immune system. (medscape.com)
  • Patients who undergo HSCT experience a sequential suppression of host defenses, resulting in varying infectious risk at different phases of the transplantation process. (medscape.com)
  • Here, we present a case of an over 60-years old male patient with AML, and the possibility for him to receive an allogeneic hematopoietic stem cell transplantation (allo-HSCT). (pensoft.net)
  • The main research focus of our laboratory is the study of immune reconstitution after hematopoietic stem cell transplantation (HSCT) in humans and the development of strategies that modulate immune responses and tolerance post-transplant. (ulisboa.pt)
  • 2020/2022 CMV infection and CMV-specific T-cell reconstitution in unrelated donor allo-HSCT after in vivo T-cell depletion with ATG: a comparative study of prophlylaxis with letermovir versus standard of care preemptive. (ulisboa.pt)
  • The Hematopoietic stem cell transplantation (HSCT) is used in children as a definitive treatment for various oncological, immune deficiencies, hemoglobinopathy, and malignancies diseases that involve the hematological system, congenital metabolism disorders, among others. (bvsalud.org)
  • This is a quantitative, retrospective, observational, descriptive and analytical quantitative approach approaching the medical records of children and adolescents submitted to HSCT in a referral hospital service for this type of transplantation in the state of Rio Grande do Sul North (RN). (bvsalud.org)
  • The allogeneic HSCT was the most frequently performed (57.14%) and the most used source of Hematopoietic progenitor cells (HPC) was the peripheral blood (54.29%) and 5.71% of these patients developed the Graft versus Host Disease (GVHD), of which one was affected by acute GVHD and another by chronic GVHD. (bvsalud.org)
  • The profile of the clinical variables presented by the children and adolescents of this study shows that the most prevalent diagnosis was ALL, the most frequent toxicities were gastrointestinal, cardiac, respiratory and hematological, the most common HSCT was allogeneic peripheral blood and the greatest cause of mortality was sepsis. (bvsalud.org)
  • Hematopoietic stem cell transplantation not responded to other therapeutic modalities, with (HSCT) is used in children as a definitive treatment an increase in survival after transplantation, for different oncological, immune deficiencies, contributing to its use (YEILIPEK, 2014). (bvsalud.org)
  • Thus, we studied the safety, efficacy, and feasibility of transfer of gene modified donor T-cells shortly after allo-HSCT in two clinical trials between 2002 and 2007 and here we compare the results to unmodified donor leukocyte infusion (DLI). (frontiersin.org)
  • The aim of these trials was to provide patients with the protection of T-cells after T-cell-depleted allo-HSCT in the matched or mismatched donor setting with an option to delete transduced T-cells, if severe aGvHD occurred within the trial period. (frontiersin.org)
  • Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is applied successfully to the treatment of many hematopoietic malignancies, but remains limited by severe acute graft-versus-host-disease (aGvHD). (frontiersin.org)
  • Recent advances in flow cytometric and molecular measurable residual disease detection have further enhanced modern risk-stratified approaches to chemotherapy and allocation to allogeneic hematopoietic stem cell transplantation (HSCT) in first complete remission (CR1) when indicated. (haematologica.org)
  • Given the potential importance of MAIT cells in control of microbial infections and protection of epithelial surfaces, we investigated MAIT cell reconstitution in 43 children who underwent hematopoietic stem cell transplantation (HSCT) after myeloablative conditioning for the treatment of malignant hematological disease. (cryostem.org)
  • In the context of allogeneic hematopoietic stem cell transplantation (allo-HSCT), this review outlines and analyzes recent developments in the understanding of the properties of tissue-resident memory T cells (TRMs). (blood-genomics.com)
GVHD21
  • Ex vivo TCD is predominantly used in GVHD prevention, where it offers the best results. (wikipedia.org)
  • Early on it was apparent that TCD was good for preventing GVHD, but also led to increased graft rejection, this problem can be solved by transplanting more hematopoietic stem cells. (wikipedia.org)
  • Graft-versus-host disease (GVHD) remains a significant complication in patients undergoing allogeneic stem cell transplantation (SCT) using a reduced intensity conditioning regimen. (qub.ac.uk)
  • Although T-cell depletion (TCD) reduces the risk of GVHD after a myeloablative conditioning regimen, it is associated with an increased risk of graft failure. (qub.ac.uk)
  • In GVHD, the donated stem cells view the recipient cells as foreign and attack recipient cells. (medscape.com)
  • Transplants from HLA-matched siblings are associated with a lower risk of GVHD and faster recovery of the recipient's immune system following transplantation. (medscape.com)
  • GVHD has been reported after syngeneic and autologous transplantation but is rare. (medscape.com)
  • Criteria used by hematologists/oncologists to diagnose acute graft-versus-host disease (GVHD), and common therapies used for GVHD prevention in patients who receive hematopoietic stem cell transplantation. (targetedonc.com)
  • We are particularly interested in developing strategies that may be translated into the clinical setting, such as the use of pathogen-specific T cells, donor regulatory T cells for GVHD and disease-specific T cells, with the aim of improving patient survival. (ulisboa.pt)
  • T-cell depletion is an approach that enhances procedure tolerability by reducing acute and chronic GVHD related morbidity and mortality. (biomedcentral.com)
  • We have employed in vivo T-cell depletion with alemtuzumab for over a decade at the University of Chicago and confirmed lower rates of acute and chronic GVHD with similar overall survival to T cell repleted HCT [ 10 ]. (biomedcentral.com)
  • Thus, prophylactic transfusion of donor T-cells has been included in many protocols, despite the increased risk for acute GvHD ( Kolb, 2008 ). (frontiersin.org)
  • The spleen also uniquely contributes toGraft Versus Host Disease (GVHD) complete B cell memory [11]. (fliphtml5.com)
  • Our hypothesis about a splenic stem cell contributionor maintained survival compared to bone marrow transplants, to PBSCT also derives from the observation that G-CSF mobilizationsalthough graft versus host disease (GVHD) still occurs [1]. (fliphtml5.com)
  • The use of allogeneic hematopoietic cell transplantation (HCT) is the most potent immunotherapeutic treatment for hematologic diseases, but its practical use is impeded by acute and chronic graft-versus-host disease (GvHD). (cryostem.org)
  • Adoptive Allogeneic T-cell therapy (ATCT) might be curative for MM, however current ATCT protocols often lead to graft versus host disease (GvHD). (biomedcentral.com)
  • Transplanting only tumor reactive donor T cells that mediate a graft-versus-myeloma (GvM) but not GvHD may overcome this problem. (biomedcentral.com)
  • These data demonstrate that a transplantation protocol involving only selective tumor-reactive donor T cell families is an effective immunotherapy and results in long-term survival in a mouse model of human MM. The results highlight the need to develop similar ATCT strategies for MM patients that result in enhanced survival without symptoms of GvHD. (biomedcentral.com)
  • Since GvT responses involve T-cell recognition of tumor-specific peptides presented by MHC molecules [ 14 ], it may be possible to identify and select donor T cells that provide beneficial GvT responses but minimal GvHD risk. (biomedcentral.com)
  • By then infusing the animals with B10.D2 T cells from only the TCR Vβ 2, 3 and 8.3 families appropriately pre-activated in vitro, we saw a vigorous GvM response without any clinical or histological signs of GvHD or disease relapse, which translated into long-term, disease-free survival. (biomedcentral.com)
  • We have a series of clinical trials available to evaluate several different strategies to prevent GVHD, such as T cell depletion and the use of novel immunosuppressive agents. (harvard.edu)
Hematopoietic stem4
Acute myeloid3
  • However, complete TCD via ex vivo, especially in acute myeloid leukemia (AML), patients usually does not improve survival. (wikipedia.org)
  • The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
  • Allogeneic marrow transplantation in patients with acute myeloid leukemia in first remission: a randomized trial of two irradiation regimens. (sagepub.com)
Peripheral14
  • The stem cell source may be bone marrow, peripheral blood, or umbilical cord blood. (medscape.com)
  • Furthermore, we have developed an automated process with selection techniques to generate CAR-T cells from umbilical cord blood T cells as well as conventional peripheral blood lymphocyte harvests from healthy donors. (ukri.org)
  • Peripheral blood has largely replaced bone marrow as a source of stem cells, especially in autologous hematopoietic stem cell transplantation, because stem cell harvest is easier and neutrophil and platelet counts recover faster. (msdmanuals.com)
  • The patient received a 7/8 HLA-matched allogeneic peripheral blood SCT (with a single mismatch at the DRB1 locus) from an unrelated donor. (cdc.gov)
  • After UMSC transplantation (UMSCT), the frequencies of Tregs and CD4 + memory T cells were significantly increased, and the frequencies of T helper (Th) 17 and CD4 + naive T cells were significantly decreased in peripheral blood (PB) of psoriasis patients. (nature.com)
  • Mature B-cell-derived cells progressively accumulate in the patients' peripheral blood, lymphatic tissues, and bone marrow. (ovgu.de)
  • These drugs strongly inhibit HIV-1 replication in a GFP indicator T cell line and peripheral blood mononuclear cells (PBMC). (biomedcentral.com)
  • Citation: Mera T, Heimfeld S, Faustman DL (2014) The Spleen Contributes Stem Cells to Peripheral Blood Stem Cell Transplants. (fliphtml5.com)
  • G-CSF was found to mobilize both CD34+ stem cells (p=0.02)and even more dramatically mobilize Hox11+ splenic stem cells (p=0.000013) into the peripheral blood. (fliphtml5.com)
  • TheIntroduction later step is unique to splenic function since splenectomy results in similar accumulations of naïve B cells, reduction of memory B cells and Treatment of malignancies with allogeneic peripheral blood stem well-known susceptibilities to select infections [12]. (fliphtml5.com)
  • 1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
  • These primitive cells undergo division and differentiation to form the various peripheral blood cells. (nationalacademies.org)
  • Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • An inflammatory checkpoint regulates recruitment of graft-versus-host reactive T cells to peripheral tissues[J]. J Exp Med , 2006, 203(8): 2021−2031. (blood-genomics.com)
Marrow Transplantation5
Graft24
  • Ex vivo techniques enable a more accurate count of the T cells in a graft and also has the option to 'addback' a set number of T cells if necessary. (wikipedia.org)
  • Another method is addition of cells back into the graft, after a comprehensive TCD method, examples are re-introduction of natural killer cells (NK), γδ T-cells and T regulatory cells (Tregs). (wikipedia.org)
  • These veto cells can also exert graft vs tumor effect. (wikipedia.org)
  • However, T-cell depletion is associated with higher rates of graft rejection and increased vulnerability to viral and fungal infections while the T-cell population is diminished. (medscape.com)
  • Although uncommon, graft failure is a severe complication following allogeneic haematopoietic stem cell transplantation (aHSCT) in paediatric patients. (bjh.be)
  • In this retrospective analysis, paediatric AML/ALL patients who were treated with an ex vivo T-cell depleting protocol and were subsequently treated with aHSCT, being under the age of 9 and having a CD8+ concentration ≥150 μL was associated with a higher risk of graft failure. (bjh.be)
  • Risk factors and survival of graft failure in pediatric patients with hematological malignancies treated with allogeneic hematopoietic stem-cell transplantation from haploidentical donors using exviv T-cell-depletion. (bjh.be)
  • If you're doing an in vivo or ex vivo T-cell depletion, the incidence of graft-versus-host disease is going to be lower than when you do a T-cell-replete, for which you leave the T cells in the graft, or you don't try to significantly remove them afterward using monoclonal antibodies targeting T cells. (targetedonc.com)
  • Therefore, the immune cells that are passively transferred in the stem cell graft are more primed against these minor histocompatibility antigens that are known to be associated with the development of acute graft-versus-host disease. (targetedonc.com)
  • Another more frequently used method to prevent graft-versus-host disease is post-transplantation cyclophosphamide. (targetedonc.com)
  • The other major way to prevent graft-versus-host disease is through T-cell depletion, and that can be ex vivo, where the graft, before it's infused, goes through a process, usually through a selection column to remove T cells from the graft. (targetedonc.com)
  • 2015/2021 Repair of tissue and organ damage in refractory chronic graft versus host disease after hematopoietic stem cell transplantation by the infusion of purified allogeneic donor regulatory T lymphocytes. (ulisboa.pt)
  • Graft-versus-tumor effects after allogeneic hematopoietic cell transplantation with nonmyeloablative conditioning. (sagepub.com)
  • Much of the curative potential of allografts is attributed to the graft-versus-tumor (GvT) response that aims to destroy residual tumor cells that persist after induction therapy and ASCT [ 5 ]. (biomedcentral.com)
  • The novel Cymerus™ manufacturing facilitates production of a virtually limitless supply of consistent human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells, which could play a key role in selective immunosuppression and graft repair during rejection. (biomedcentral.com)
  • We demonstrated that iPSC-derived MSC treatment leads to significant increases in hTSG-6 protein, followed by an upregulation of mouse Tregs and IL-5, IL-10, and IL-15 cytokines, which augments graft microvascular blood flow and oxygenation, and thereby maintained a healthy airway epithelium and prevented the subepithelial deposition of collagen at d90 post transplantation. (biomedcentral.com)
  • Existing immunosuppressive drugs for organ transplantation may achieve sufficient immunosuppression to prevent organ rejection or limit autoreactivity, but they are typically not successful in achieving long-term survival of the graft or preventing progression of fibrosis and chronic rejection [ 3 ]. (biomedcentral.com)
  • In addition, we are committed to graft engineering and vaccine development to enhance the ability of transplantation to control blood diseases. (harvard.edu)
  • The link between tissue-resident memory T cells and graft-versus-host disease[J]. Blood&Genomics, 2022, 6(2): 81-90. (blood-genomics.com)
  • A crucial role for antigen-presenting cells and alloantigen expression in graft-versus-leukemia responses[J]. Nat Med , 2005, 11(11): 1244−1249. (blood-genomics.com)
  • Fefer A. Graft-vs. -tumor responses[M]// Thomas' hematopoietic cell transplantation , New Jersey: Blackwell Publishing Ltd, 2003: 369-379. (blood-genomics.com)
  • Graft-vs-lymphoma effect in an allogeneic hematopoietic stem cell transplantation model[J]. Biol Blood Marrow Transplant , 1999, 5(6): 357−368. (blood-genomics.com)
  • Tissue-resident PSGL1 lo CD4 + T cells promote B cell differentiation and chronic graft-versus-host disease-associated autoimmunity[J]. J Clin Invest , 2021, 131(1): e135468. (blood-genomics.com)
  • Spatiotemporal single-cell profiling reveals that invasive and tissue-resident memory donor CD8 + T cells drive gastrointestinal acute graft-versus-host disease[J]. Sci Transl Med , 2021, 13(576): eabc0227. (blood-genomics.com)
Haematopoietic2
  • Beyond T cell/CAR therapies, the terminal-CRISPR vector configuration is being applied to a correction of single gene disorders of the haematopoietic system and skin disorders, and will be of broad interest to the field. (ukri.org)
  • Haematopoietic cell transplantation as immunotherapy[J]. Nature , 2001, 411(6835): 385−389. (blood-genomics.com)
Donors9
  • Donors for allogeneic transplants may be related or unrelated to the recipient. (medscape.com)
  • Allogeneic hematopoietic stem cell transplantation is limited mainly by lack of histocompatible donors. (msdmanuals.com)
  • Treatment for acute myelogenous leukemia by low-dose, total-body, irradiation-based conditioning and hematopoietic cell transplantation from related and unrelated donors. (sagepub.com)
  • Transduced cells were transfused either after day +60 (matched donors) or on day +42 (haploidentical donors). (frontiersin.org)
  • For induce splenomegaly in most donors and in rare, severe cases splenicautologous stem cell transplants, the use of autologous PBSCT from rupture [13,14]. (fliphtml5.com)
  • Results Natural killer cells from healthy donors and myeloma patients expanded a median of 804- and 351-fold, respectively, without significant T-cell expansion. (haematologica.org)
  • 2003). After the early success of transplantation of cord blood from related donors, cord blood banks were established to provide rapidly accessible, human leukocyte antigen (HLA)-typed units predominantly for transplantation of HPCs from unrelated donors. (nationalacademies.org)
  • Blood and Marrow Transplant Research [CIBMTR] in 2004), the European Research Project on Cord Blood Transplantation (Eurocord) in 1993, and the Japanese Cord Blood Banking Network in 1996-expedited the clinical evaluation of the efficacy and safety of transplantation of cord blood from unrelated donors. (nationalacademies.org)
  • Among PSCs, the donors available for induced pluripotent stem cells (iPSCs) are greatest, providing a potentially universal cell source for all types of cell therapies including cancer immunotherapies using natural killer (NK cells). (molcells.org)
Bone13
  • Autologous transplantation is feasible when the patient's bone marrow is normal and there are no relevant genetic conditions. (medscape.com)
  • AML is a type of blood cancer with excess immature white blood cells in bone marrow. (pensoft.net)
  • Bidirectional interaction between CLL cells and the surrounding non-transformed stromal compartment (especially in the bone marrow) and extracellular matrix components extend CLL survival and protect from chemotherapeutics. (ovgu.de)
  • Similarly to the bone marrow stroma, ECs express several surface markers that may allow cell-to-cell contact between them and CLL cells (e.g. (ovgu.de)
  • A.) CLL cells display a recurrent and often yearlong contact to the epithelial barrier during circulation and/or transendothelial migration into e.g., the bone marrow or spleen. (ovgu.de)
  • The findingssupport the hypothesis that G-CSF mobilizes two distinct stem cell populations, one from the bone marrow andthe other from the spleen. (fliphtml5.com)
  • These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
  • The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
  • Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)
  • What is radically new and is causing a change in the way we view health and wellness is the discovery that adult stem cells in the bone marrow have the ability to become virtually any kind of cell types in the body. (myradiary.com)
  • Mesenchymal stem cells (MSCs) are adult stem cells that can be isolated from most adult tissues, including bone marrow, adipose, liver, amniotic fluid, lung, skeletal muscle and kidney. (myradiary.com)
  • and hence being tested in four main areas: tissue regeneration for cartilage, bone, muscle, tendon and neuronal cells for treatment of immune diseases such as RA, MS, etc. (myradiary.com)
  • B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
Mesenchymal stem5
  • Mesenchymal stem cells (MSCs) have immunomodulatory properties. (nature.com)
  • With other REMEDI researchers we are specifically studying the immune therapeutic properties of mesenchymal stem cells (MSC). (universityofgalway.ie)
  • To learn more about stem cells, do watch this video in which Dr. Riordan discusses about mesenchymal stem cells (MSCs) harvested from fat tissue and the role they play in reducing inflammation, repairing tissue and modulating the immune system. (myradiary.com)
  • mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
Donor12
  • Majority of patients (97%) were taking newer tri-azole prophylaxis (posaconazole) as per standard centre protocol.Results: Pre-IFD cases were similar to those without pre-IFD in gender, age, disease type, stem cell source, conditioning regimen and donor source whereas conditioning intensity, donor matching and HCTCI scores were significantly different. (kcl.ac.uk)
  • In autologous transplantation, the donor and recipient is the same individual. (medscape.com)
  • When the donor is someone other than the recipient, the procedure is described as allogeneic transplantation. (medscape.com)
  • Allogeneic transplants are further categorized by the degree of human leukocyte antigen (HLA) match between the donor and recipient. (medscape.com)
  • We recently showed that its possible not only to arm cells against leukaemia but also disarm them to stop side effects if they are taken from a healthy donor and transplanted to a patient without any matching. (ukri.org)
  • Hematopoietic stem cell transplantation may be autologous (using the patient's own cells) or allogeneic (using cells from a donor). (msdmanuals.com)
  • The treatment options for disease relapse after HCT include withdrawal of immune suppression, chemotherapy, second allogeneic transplant, cytokine and adoptive cell therapy and donor lymphocyte infusion [ 1 ]. (biomedcentral.com)
  • Donor-T-cells were transduced with the replication-deficient retrovirus SFCMM-3, expressing HSV-TK and the truncated ΔLNGFR for selection of transduced cells. (frontiersin.org)
  • Donor chimerism was stabilized after transfusion of the transduced cells in all patients treated. (frontiersin.org)
  • The implication of these results would be that myeloma-specific T cell subfamilies might be positively selected from the donor inoculum and infused to myeloma patients post ASCT, to afford separation of allo- from tumor-reactive T cells without the prior need to define specific target antigens. (biomedcentral.com)
  • Donor Clonal Hematopoiesis and Recipient Outcomes After Transplantation. (harvard.edu)
  • The only curative treatment for ALF is liver transplantation, but there are many restrictions on the application of liver transplantation because of financial considerations, a shortage of donor livers, and immunosuppression-related complications [ 3 ]. (springer.com)
Organ Transplantation2
Haploidentical5
Transplant17
  • Risks of infection also vary with the type of transplant, the indication for transplantation, and other host factors. (medscape.com)
  • Although further prospective research is required to validate these findings, these results highlight the significance of pre-existing cellular immunity and support the use of T-cell population analysis in the pre-transplant patient workup. (bjh.be)
  • We report a case of disseminated microsporidiosis in a patient with multiple myeloma who had received an allogeneic stem cell transplant requiring substantial immunosuppression. (cdc.gov)
  • in an immunosuppressed patient who received an allogeneic stem cell transplant for multiple myeloma. (cdc.gov)
  • After receiving an autologous stem cell transplant (SCT), she experienced a relapse of disease and was enrolled in an experimental protocol of immunoablative chemotherapy, followed by hematopoietic SCT at the National Institutes of Health Clinical Center in Bethesda, Maryland ( www.ClinicalTrials.gov identifier NCT00520130). (cdc.gov)
  • Microsporidium detected in clinical specimens from a stem cell transplant patient who had undergone substantial immunosuppression. (cdc.gov)
  • Second allogeneic hematopoietic stem cell transplant (HCT) remains as an option for disease relapse after initial HCT. (biomedcentral.com)
  • Although it is proving to be a heterogeneous disease process, the only treatment with proven survival benefit for poor risk AML remains allogeneic hematopoietic cell transplant. (sagepub.com)
  • The immunosuppressants mycophenolate mofetil (MMF) and cyclosporine were given to the patient to prevent rejection of the allogeneic stem cell transplant, whereas the chemotherapeutic agent cytarabine was utilized to kill leukemic cells. (biomedcentral.com)
  • My interest in transplantation resulted in a research fellowship in basic immunology at the University of Chicago, USA following which I spent 9 years combining clinical transplant medicine with laboratory-based immunology research at Mayo Clinic. (universityofgalway.ie)
  • Prior to transplant, the woman was given several drugs to allow her body to accept the new cells. (myradiary.com)
  • BALB/c → C57BL/6 allografts were reconstituted with iPSC-derived MSCs (2 million/transplant/at d0), and allografts were examined for regulatory T cells (Tregs), oxygenation, microvascular blood flow, airway epithelium, and collagen deposition during rejection. (biomedcentral.com)
  • Transplant Cell Ther 2021. (harvard.edu)
  • Defibrotide: Real World Management of Veno-Occlusive Disease/ Sinusoidal Obstructive Syndrome after Stem Cell Transplant. (harvard.edu)
  • incidence and predictors of post-transplant lymphoproliferative disease after kidney transplantation during adulthood and childhood: a registry study. (liverpool.ac.uk)
  • All three of the responders failed multiple lines of prior therapy including chemotherapy, CD19-directed autologous CAR T cell therapy, and allogeneic stem cell transplant. (cellectis.com)
  • These treatment responses in combination with the safety data are very encouraging for patients with r/r B-cell ALL who have limited, if any, treatment options, especially for those who have failed prior CD19 directed CAR T-cell therapy and allogeneic stem cell transplant', said Nitin Jain, M.D., The University of Texas MD Anderson Cancer Center, Department of Leukemia, and coordinating investigator for the BALLI-01 study. (cellectis.com)
Malignancies7
  • Other leukaemia including T cell malignancies and acute myeloid leukaemia should be amenable to similar approaches. (ukri.org)
  • More recently, we engaged in developing several novel strategies for CAR T cell therapies of hematologic malignancies and solid tumors. (ulisboa.pt)
  • Funding Agency: Fellowship Building the future knowledge in mature B cell malignancies attributed by SPH, APCL and Gilead Sciences. (ulisboa.pt)
  • Natural killer (NK) cells can kill cancer cells in the absence of prior stimulation and hold considerable potential for cell-based therapies targeting human malignancies ( 1 - 4 ). (aacrjournals.org)
  • NCI First International Workshop on The Biology, Prevention and Treatment of Relapse after Allogeneic Hematopoietic Cell Transplantation: report from the committee on prevention of relapse following allogeneic cell transplantation for hematologic malignancies. (sagepub.com)
  • CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. (thermofisher.com)
  • adoptive transfer of genetically engineered t-cells to express antigen-specific t-cell receptor (tcr) is a feasible and effective therapeutic approach for numerous types of cancers, including epstein-barr virus (ebv)-associated malignancies. (liverpool.ac.uk)
Immunotherapy4
  • Infusions of natural killer (NK) cells are an emerging tool for cancer immunotherapy. (aacrjournals.org)
  • Stem cell-based immunotherapy has been recognized as a crucial immunoregulatory regimen in various preclinical and clinical studies. (biomedcentral.com)
  • Cell-mediated immunotherapy is emerging as an alternative in single and combination therapies to rescue rejecting transplants. (biomedcentral.com)
  • here, we describe a tcr gene transfer regimen to rapidly and reliably generate t-cells specific to ebv-encoded latent membrane protein-1 (lmp1), which is a potential target for t-cell-based immunotherapy. (liverpool.ac.uk)
Relapse2
Multiple Myeloma2
Vitro9
  • These techniques can be performed either in vivo, ex vivo, or in vitro. (wikipedia.org)
  • In contrast, both in vivo TCD with alemtuzumab and in vitro TCD with CD34+ selection performed comparably. (wikipedia.org)
  • We showed that functional blockade of CD200 on lymphoma cells or primary CLL cells, both of which express CD200 at high levels, augmented cytotoxic killing of these cells by effector CD8+ T cells in vitro. (ndltd.org)
  • We will employ different methods for metabolic profiling using an in vitro co-culture model (cooperation with Project 2 ) including single-cell RNA sequencing (cooperation with Project 12 ), targeted metabolomics, and flow cytometry-based analyses of metabolic markers/parameters as well as intracellular signaling components (focus on NF-κB signaling in cooperation with Project 1 ). (ovgu.de)
  • Knockdown of CASP5 greatly inhibited GBM proliferation and resulted in G1 cell cycle arrest along with higher apoptosis ratios in vitro and in vivo, while overexpression led to the opposite phenomenon. (cancerindex.org)
  • We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
  • Although liver failure can be treated via hepatocyte transplantation, it also faces multiple problems comprising the shortage of high-quality hepatocytes sources, rejection of allogeneic transplants, difficulty to expand, and losing hepatic characteristics in vitro [ 7 , 8 ]. (springer.com)
  • Previous studies have proven that rodent and human MSCs can differentiate into HLCs in vitro and in vivo , which is one of the mechanisms of MSCs in the treatment of liver failure. (springer.com)
  • The results of in vitro C3 uptake studies and the effect of IVIG on the clearance of preopsonized cells suggest that IVIG produces a kinetic depression of C3 uptake and modifies the process of complement fragment deposition on erythrocytes. (medscape.com)
Aplastic Anemia1
  • Over-activated T-cells will cause immune-related adverse events such as Aplastic Anemia (AA). (pensoft.net)
Rejection1
  • This procedure is called 'megadose transplantation' and it prevents rejection because the stem cells have an ability (i.e. veto cell killing) to protect themselves from the host's immune system. (wikipedia.org)
Umbilical3
Refractory3
  • We now propose a Phase 1 study to treat children with relapsed refractory CD19+ leukaemia as a proof of concept application of CRISPR genome modified T cells. (ukri.org)
  • Around 10-15% go on to develop relapsed refractory cases and may fail to respond to aggressive treatment, including allogeneic stem cell transplantation. (ukri.org)
  • In the journal, Induction of remission of severe and refractory rheumatoid arthritis by allogeneic mixed chimerism , by Burt et al, American College of Rheumatology, first published in 2004, it was reported that researchers from Northwestern University in Chicago alleged that a 52-years old woman with RA in 38 joints has successfully been treated with stem cells from a sibling. (myradiary.com)
Regulatory T cel3
  • Depletion of regulatory T cells increases immune activation. (wikipedia.org)
  • In a mammary carcinoma model, depletion of CD25+ regulatory T cells increase the amount of CD8+CD11c+PD110, which target and kill the tumors. (wikipedia.org)
  • 2021/2024 Generation and in-depth characterization of antigen-specific regulatory T cells to be applied in allogeneic hematopoietic stem transplantation. (ulisboa.pt)
Monoclonal antibodies2
  • In vivo depletion often uses monoclonal antibodies (eg, alemtuzumab) or heteroantisera. (wikipedia.org)
  • We found this is at least partly mediated via bioenergetic reprogramming and formation of immune escape variants, both of which are of particular importance for drug sensitivity and immunotherapeutic concepts including among others monoclonal antibodies or genetically engineered CAR T-cells. (ovgu.de)
Tregs2
  • And all responders showed significant increases in Tregs and CD4 + memory T cells, and significant decreases in Th17 cells and serum IL-17 level after UMSCT. (nature.com)
  • In conclusion, allogeneic UMSCT is safe and partially effective in psoriasis patients, and level of Tregs may be used as a potent biomarker to predict the clinical efficacy of UMSCT. (nature.com)
Lymphocytes1
Immunology2
Differentiation2
  • When genetically susceptible individuals are exposed to infection, stress or trauma, cutaneous dendritic cells are activated to produce tumor necrosis factor (TNF)-α and interleukin (IL)-23, which subsequently stimulate the proliferation and differentiation of proinflammatory T cells. (nature.com)
  • Accordingly, scientists have developed protocols for the expansion, genetic modification and differentiation of stem cells to NK cells. (molcells.org)
Infusion4
  • The harvested stem cells are processed before infusion into the recipient. (medscape.com)
  • The methotrexate is usually given on days 1, 3, 6, and 11 following the stem cell infusion. (targetedonc.com)
  • Infusion of sCD200hi CLL plasma, but not sCD200lo normal plasma, enhanced engraftment of CLL-splenocytes in vivo, an effect which was abrogated by depletion of sCD200 from CLL plasma. (ndltd.org)
  • Repeated infusion of naïve myeloma-reactive T cell TCRVβ 2, 3 and 8.3 subfamilies was also effective. (biomedcentral.com)
20221
  • 2022. Human embryonic stem cell-derived dopaminergic grafts alleviate L-DOPA induced dyskinesia . (cardiff.ac.uk)
Clinical6
  • We therefore adapted the K562-mb15-41BBL stimulation method to large-scale clinical-grade conditions, generating large numbers of highly cytotoxic NK cells. (aacrjournals.org)
  • The results that we report here provide rationale and practical platform for clinical testing of expanded and activated NK cells for cell therapy of cancer. (aacrjournals.org)
  • Enrichment of PBSCT by purification of CD34+stem cells fails to produce superior clinical benefits. (fliphtml5.com)
  • The inferior clinical performance of CD34+-enriched and purified PBSCTs compared tounenriched PBSCTs may be explained by the omission of Hox11+ stem cells. (fliphtml5.com)
  • 9. Clinical complications and outcomes of human kidney and pancreas transplantation. (universityofgalway.ie)
  • Compared to the last clinical update on BALLI-01 at ASH 2021, the webcast presented data from five additional patients who received UCART22 at dose level 3 (DL3) 5x106 cells/kg after lymphodepletion with FCA. (cellectis.com)
Proliferation3
  • Splenomegaly might reflect dramatic G-CSF-inducedG-CSF stimulation also in multiple studies shows faster recovery of Hox11+ stem cell proliferation. (fliphtml5.com)
  • CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. (thermofisher.com)
  • latent infection can spread by b cell recirculation and proliferation, but whether this alone achieves systemic infection is unclear. (liverpool.ac.uk)
Chemotherapy2
  • Laboratory investigations revealed white blood cell count (WBC) 44×109/L with 86% blasts, hemoglobin 8.1 g/dL and a platelet count of 37×109/L. After obtaining an informed consent, the patient was treated with an induction chemotherapy with IA regimen (cytarabine 1.5 g/m2 by vein daily on days 1-3, with idarubicin 12 mg/m2 by vein daily on days 1-3). (pensoft.net)
  • Hematopoietic stem cell transplantation is also sometimes used for solid tumors (eg, some germ cell tumors) that respond to chemotherapy. (msdmanuals.com)
Remission1
Patients11
  • Antiretroviral therapy, the most common treatment for patients with HIV, has been shown to restore CD4+ T cell counts. (wikipedia.org)
  • Fifteen patients have been transplanted using such a regimen of whom 13 underwent ex vivo TCD. (qub.ac.uk)
  • The toxicities are observed in more than 10% of patients, because of its ability, anti-PD-1 will upregulate the activity of T-cells. (pensoft.net)
  • Currently, there are no known risk factors to identify paediatric patients who may be at a greater risk of this complication when patients are treated with an ex vivo T-cell depletion protocol beforehand. (bjh.be)
  • All patients received a T-cell depletion protocol consisting of fludarabine, busulfan and thiotepa. (bjh.be)
  • Most of the trials so far have relied in collecting a patients own white cells and then using a disabled virus to fit new genes into a group of cells called T cells. (ukri.org)
  • This process then arms the cells against leukaemia, so when they are given back to the patients they can attack the disease. (ukri.org)
  • All except four patients received T cell depleted (TCD) first HCT. (biomedcentral.com)
  • To date, six patients have relapsed and died, two after a second hematopoietic stem cell transplantation without T-cell depletion or administration of unmodified T-cells. (frontiersin.org)
  • Lung transplantation is a life-saving surgical replacement of diseased lungs in patients with end-stage respiratory malfunctions. (biomedcentral.com)
  • Lung transplantation is a life-saving surgical procedure for patients with end-stage lung diseases. (biomedcentral.com)
Responses7
  • T-cell depletion (TCD) is the process of T cell removal or reduction, which alters the immune system and its responses. (wikipedia.org)
  • One example is antigen-specific CD4+ T cell tolerance, which serves as the primary mechanism restricting immunotherapeutic responses to the endogenous self antigen guanylyl cyclase c (GUCY2C) in colorectal cancer. (wikipedia.org)
  • However, in some cases, selective CD4+ T cell tolerance provides a unique therapeutic opportunity to maximize self antigen-targeted immune and antitumor responses without inducing autoimmunity by incorporating self antigen-independent CD4+ T cell epitopes into cancer vaccines. (wikipedia.org)
  • The ability of cancer cells to escape anti-tumor immune responses is acknowledged as one of the hallmarks of cancer. (ndltd.org)
  • enhance the CLL cells' ability to suppress immune responses by e.g., substrate depletion and/or production of bioactive metabolites and it could (2. (ovgu.de)
  • In this regard, immune-transcriptome analyses of T cell receptor (TCR) Vβ CDR3-size and sequence is being used to characterize alloreactive versus tumor-specific T-cell responses. (biomedcentral.com)
  • Immunotolerance is a major immunological phase, which facilitates reparative microenvironment during transplantation, and plays a vital role to suppress alloimmune responses. (biomedcentral.com)
Receptor8
  • We undertook the first application of genome modified T cells expressing a chimeric antigen receptor (CAR) against CD19 to successfully treat infant B cell acute lymphoblastic leukaemia (B-ALL) in 2015. (ukri.org)
  • Cells were transduced with a lentiviral vector to express CAR19 and then further modified using TALENS to disrupt T cell receptor (TCR) and CD52. (ukri.org)
  • 2020/2023 Development of Chimeric Antigen Receptor T cell therapy targeting oncological-associated chondroitin sulfate as cancer treatment. (ulisboa.pt)
  • Mounting evidence also supports the pivotal role of the clonotypic B-cell receptor immunoglobulin (BcR IG) in the natural history of CLL. (ndltd.org)
  • Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
  • The mucosa-associated invariant T (MAIT) cells are innate-like T cells with restricted T cell receptor (TCR) usage, which are preferentially localized in mucosal tissues (liver, lung and gut) and respond to microbial infection by rapidly producing cytokines and cytotoxic effectors. (cryostem.org)
  • CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. (thermofisher.com)
  • After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. (thermofisher.com)
Cytokine3
  • cytokine levels cause a decline in cell numbers. (123dok.net)
  • In certain circumstances, MAIT cells are also able to produce IL-22, a cytokine involved in gut epithelial protection during inflammatory intestinal damage. (cryostem.org)
  • No dose limiting toxicities (DLTs), Grade 2 or higher cytokine release syndrome (CRS), immune effector cell-associated neurotoxicity syndrome (ICANS) or adverse events of special interest (AESI) were observed. (cellectis.com)
Adoptive2
  • Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
  • Adoptive cell transfer (ACT) is the latest arsenal in the battle against cancer. (molcells.org)
Therapies2
  • Universal T cells aim to circumvent HLA barriers and provide an 'off-the-shelf' alternative to autologous cell therapies. (ukri.org)
  • Last, we will interfere with the identified (metabolic) mechanisms in vivo using a preclinical model and evaluate the impact on the CLL cell metabolism, T cell compartment, and response to therapies. (ovgu.de)
Leukemia1
  • We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
Curative treatment2
Renal1
  • long-term follow up of post renal transplantation epstein-barr virus-associated smooth muscle tumors: report of two cases and review of the literature. (liverpool.ac.uk)
Humans1
  • Conclusions These studies provide the rationale for testing expanded natural killer cells in humans. (haematologica.org)
Patient's1
  • After the first week of treatment, the patient's WBC count fell to 36×109/L with a significant decrease in blast cell count. (pensoft.net)
Myeloma cells1
  • To test this rationale, we used the allogeneic B10.D2 → Balb/c BMT model with MOPC315.BM myeloma cells. (biomedcentral.com)
Treatment5
  • Depletion can occur naturally (i.e. in HIV) or be induced for treatment purposes. (wikipedia.org)
  • In the meantime, improvements in the way we modify cells have led to the development of new versions of the treatment that we think are going to be more widely used. (ukri.org)
  • 1-3 This has downscaled the role of allogeneic hematopoietic cell transplantation (allo-HCT) as the formerly most effective treatment of HR-CLL. (ashpublications.org)
  • Can Stem Cell Therapy be a Viable Rheumatoid Arthritis Treatment? (myradiary.com)
  • Indirectly, it also leads me to think about whether stem cell therapy can be a viable alternative treatment for Rheumatoid Arthritis at all. (myradiary.com)
Progenitor cells2
  • As the cells reproduce, they commit to a particular task or cell line and become known as committed progenitor cells . (nationalacademies.org)
  • These committed progenitor cells are difficult to discern from the original multipotent cells but can be cultured to form colonies of specific types of blood cells (Guyton and Hall, 2000). (nationalacademies.org)
Human-murine2
  • In vivo, human-murine anti-leukaemia modelling has confirmed functional integrity of CAR+TCR- cells, and found these were less prone to exhaustion compared to TCR+ cells. (ukri.org)
  • First, we will analyze the metabolic phenotype and the underlying signaling pathways of human/murine CLL cells in response to their interaction with ECs. (ovgu.de)
MSCs4
  • MSCs are multipotent cells characterized by their regenerative capabilities and immunomodulatory properties. (nature.com)
  • 11 The regulatory effect of MSCs on T cells has also been demonstrated in mice models of autoimmune disease such as rheumatoid arthritis and sjogren syndrome (SS). (nature.com)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • Therefore, MSCs may help resolve issues restricting the application of hepatocyte transplantation. (springer.com)
Immune system3
  • This problem is partially answered by more selective depletion, such as depletion of CD3+ or αβT-cell and CD19 B cell, which preserves other important cells of the immune system. (wikipedia.org)
  • However, over time, an individual's immune system can no longer continue to replace CD4+ T cells. (wikipedia.org)
  • 2 Psoriasis is a disease with innate and adaptive immune system disorder and dendritic cells, neutrophils, keratinocytes and T cells play major roles in the pathogenesis. (nature.com)
Recipients1
  • In preparation for receipt of the stem cells, recipients undergo myeloablation to eliminate their own myeloid cells. (medscape.com)