AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, Agriculture
Hematopoietic Stem CellsStem CellsStem Cell TransplantationEmbryonic Stem CellsAdult Stem CellsPluripotent Stem CellsHematopoietic Stem Cell TransplantationStem Cell NicheNeural Stem CellsInduced Pluripotent Stem CellsCell DifferentiationMultipotent Stem CellsMesenchymal Stem Cell TransplantationStem Cell FactorMesenchymal Stromal CellsCell LineageStem Cell ResearchPlant StemsFetal Stem CellsCell ProliferationCells, CulturedHematopoietic Stem Cell MobilizationNeoplastic Stem CellsBone Marrow CellsRegenerationOctamer Transcription Factor-3Cell Culture TechniquesRegenerative MedicineCell SeparationTransplantation, AutologousHematopoiesisPeripheral Blood Stem Cell TransplantationTotipotent Stem CellsAntigens, CD34Transplantation, HomologousFlow CytometryMice, Inbred C57BLEmbryo, MammalianSOXB1 Transcription FactorsSpermatogoniaSignal TransductionColony-Forming Units AssayCell- and Tissue-Based TherapyMice, SCIDTissue EngineeringGene Expression Regulation, DevelopmentalProto-Oncogene Proteins c-kitCord Blood Stem Cell TransplantationNestinCell LineReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingGerm CellsHomeodomain ProteinsHair FollicleBrain StemEmbryo ResearchPhenotypeCell SurvivalBiological MarkersCoculture TechniquesMice, TransgenicBone Marrow TransplantationNeuronsCell DivisionCell DedifferentiationModels, BiologicalFetal BloodImmunohistochemistryTranscription FactorsGreen Fluorescent ProteinsTransplantation ConditioningBone MarrowTeratomaAntigens, CDGene Expression RegulationEmbryoid BodiesNuclear ReprogrammingSpheroids, CellularCell TransplantationNeurogenesisTime FactorsChondrogenesisLentivirusGraft vs Host DiseaseCell MovementMice, Inbred NODCell TrackingGranulocyte Colony-Stimulating FactorAntigens, CD24Tissue ScaffoldsDental PulpTransplantation, HeterologousLimbus CorneaeEmbryonal Carcinoma Stem CellsGene ExpressionCell CountStage-Specific Embryonic AntigensAdipogenesis
Vitro and in vivoDifferentiationMSCsTransplantationTumorsPluripotent cellsTherapyRegenerationMesenchymal stem cell-ProgenitorTissueBiologyAdipose-derivedEngraftmentMultipotentLund Stem Cell CenterEndogenous neural stemMouse xenograftTumor cellsEpithelialPhenotypePopulationsEmbryonic stemMaturationFateCSCsDevelopment in vivoClinicalIsolationRegenerative medicineSurvivalProliferationHumanLimbal stem2019Extracellular vesiclesFlow cytometryMechanismsMetastasisHeterogeneityMolecularNeuronalMyeloidAutologousDonorTherapeutic potentialTissuesCharacterizationCancerSelf-renewalHematopoietic stemNeuronsCorrelatesNSCs
Vitro and in vivo8
  • Detection thresholds of the labeled cells in vitro and in vivo were determined to be 10 4 and 10 5 cells, respectively. (biomedcentral.com)
  • In vitro and in vivo results implied that Cbfa1-overexpressing ASCs may be an alternative cell source for BMSCs for bone tissue engineering. (jarde.jp)
  • The present study was conducted to evaluate whether secretome derived from Human Wharton's jelly mesenchymal stem cells (hWJMSCs) may stimulate or inhibit breast cancer growth in vitro and in vivo. (ac.ir)
  • 5. Ahn J-O, Coh Y-R, Lee H-W, Shin I-S, Kang S-K, Youn H-Y. Human adipose tissue-derived mesenchymal stem cells inhibit melanoma growth in vitro and in vivo. (ac.ir)
  • Naive human umbilical cord matrix derived stem cells significantly attenuate growth of human breast cancer cells in vitro and in vivo. (ac.ir)
  • Polarized microglia had differential effects on the differentiation potential of NSCs in vitro and in vivo, with M1 microglia promoting astrocytogenesis, while M2 microglia supported neurogenesis. (biomedcentral.com)
  • Overall, this study shed light on the complex conditions governing microglia polarization and the effects of differentially polarized microglia on critical functions of NSCs in vitro and in vivo. (biomedcentral.com)
  • This also allows us the unique opportunity to directly compare our hESC-derived dopamine neurons with those sourced from human fetal tissue both in vitro and in vivo. (lu.se)
Differentiation21
  • show that teratoma formation can be minimized by transplanting the cells into an engineered niche that promotes efficient differentiation. (nature.com)
  • Accordingly, CSCs are a subpopulation of cancer cells with capacity for self-renewal, differentiation and tumor initiation. (nih.gov)
  • Moreover, in vivo evidence is lacking to fully define the reversibility of CSC differentiation. (nih.gov)
  • In a study published in the journal Blood, the Larsson group targeted the enzyme LSD1, part of the CoREST complex, known to mediate the epigenetic modification of DNA and pushing the differentiation of stem cells into mature blood cells. (lu.se)
  • We hypothesised that by reducing LSD1 levels using small molecule drugs we could halt stem cell differentiation and stimulate expansion. (lu.se)
  • We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)
  • These findings suggested that overex-pression of the Cbfa1 gene induced a remarkable and rapid differentiation of ASCs into osteogenic cells. (jarde.jp)
  • 13. Wang X, Omar O, Vazirisani F, Thomsen P, Ekström K. Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation. (ac.ir)
  • We have over-expressed SKI to define its intrinsic role in hematopoiesis and myeloid neoplasms, which resulted in a robust competitive advantage upon transplantation, a complete dominance of the stem and progenitor compartments, and a marked enhancement of myeloid differentiation at the expense of other lineages. (edu.au)
  • Accordingly, enforced expression of SKI induced a gene signature associated with hematopoietic stem cells and myeloid differentiation, as well as hepatocyte growth factor signaling. (edu.au)
  • In the 1980's, Arnold Caplan and his colleagues published an isolation method of fibroblast-like stromal cells from bone marrow and first identified them as mesenchymal stem cells (MSCs) because of their multilineage differentiation potential ( Caplan, 1991 ). (frontiersin.org)
  • The effect of external signals on myogenic differentiation of BLC6 cells was demonstrated by cocultivation with visceral endodermal END-2 cells and the activin A-secreting WEHI-3 cells. (uni-luebeck.de)
  • END-2 cells essentially prevent skeletal muscle differentiation, whereas basic fibroblast growth factor, transforming growth factor-β, and WEHI-3 cells have no or an attenuating effect, respectively. (uni-luebeck.de)
  • We demonstrate here that R246S mutant p53 exhibits DN effects with respect to target gene expression, cell survival and cell cycle arrest both in cells that are in the undifferentiated state and upon differentiation. (silverchair.com)
  • At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)
  • This study describes for the first time, a differentiation system that does not require co-culture with animal cells or products, thus allowing the production of pathogen-free RPE cells suitable for transplantation into patients. (news-medical.net)
  • Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
  • In addition, cells were assessed for multi-differentiation potential. (bvsalud.org)
  • Similarly, the cells showed differentiation ability into all three lineages of cells tested. (bvsalud.org)
  • The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
  • Through the use of human embryonic stem cells (hESCs), we can mimic brain development towards different regions of the human brain, and thereby investigate the effect of novel genes on neural differentiation. (lu.se)
MSCs15
  • Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
  • Thus, we attempted to present an overview of previously published reports to evaluate the progress and provide molecular basis of mesenchymal stem cells (MSCs) therapy and its application in preclinical and clinical settings, which could aid in designing an effective regenerative therapeutic strategy in the future. (hindawi.com)
  • However, effective dose and appropriate time of MSCs delivery are the main challenges in the clinical translation of stem cell therapy. (hindawi.com)
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)
  • While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)
  • To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)
  • Controversial results have been reported regarding the anti-tumor properties of extracellular vesicles derived from mesenchymal stem cells (MSCs). (ac.ir)
  • Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of MSCs mediated by the interaction between MSC-derived paracrine mediators and Mφs remains elusive. (frontiersin.org)
  • There are currently over 800 registered clinical trials that use mesenchymal stem cells (MSCs) for tissue repair and immunomodulation. (iwmpi.org)
  • However, the fate of MSCs in vivo including their overall biodistribution and local tissue quantities is not fully known, especially how these parameters change over time. (iwmpi.org)
  • Cell therapy using MSCs (mesenchymal stem cells) might be effective treatment for refractory GVHD (graft-versus-host disease). (ewha.ac.kr)
  • In vivo movement of the donor splenocytes (EGFP) and MSCs (RFP) were evaluated by measuring the biofluorescence (IVIS-Xenogen system). (ewha.ac.kr)
  • Recently, ex-vivo gene therapy has emerged as a promising approach to enhance the therapeutic potential of mesenchymal stem cells (MSCs) by introducing functional genes in vitro . (ijstemcell.com)
  • We evaluated the correlation between the therapeutic efficacy and the purity of therapeutic MSCs/CD by examining their anti-cancer effect on co-cultured U87/GFP cells. (ijstemcell.com)
  • In vivo, DMOG delivery significantly reduced mineralisation of the proteoglycan-rich cartilaginous tissue generated by MSCs within alginate hydrogels loaded with TGF-β3 and BMP-2. (amrita.edu)
Transplantation15
Tumors5
  • The cancer stem cell model implies a hierarchical organization within breast tumors maintained by cancer stem-like cells (CSCs). (nih.gov)
  • Our main experimental approach is based on lineage-tracing studies, to genetically label stem cells and follow their lineages and behavior in vivo during development, tissue remodeling and regeneration and in tumors. (stemcells-live.fr)
  • Accumulating data suggests that many human tumors are organized in cellular hierarchies initiated and maintained by a small population of self-renewing so called cancer stem cells. (lu.se)
  • Cells with high expression of HIF-2 α have properties rendering the tumors aggressive behavior potentially being the neuroblastoma tumor stem cells. (lu.se)
  • The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
Pluripotent cells2
  • Pluripotent cells are implanted in a hydroxyapatite-coated poly- L -lactic acid scaffold that releases bone morphogenic protein 2. (nature.com)
  • The implants achieve rapid and efficient repair of a calvarial defect with a very low incidence of teratomas (they are seen in 2 of 42 mice) after injection of 1 million human pluripotent cells. (nature.com)
Therapy17
  • However, the standardization of the manufacturing process of MSC-based cell therapy medicinal products in compliance with the requirements of the local authorities is obligatory and will allow us to obtain the necessary permits for product administration according to its intended use. (mdpi.com)
  • However, understanding the mode of action of the cell therapy has been limited by cell tracking capability and correlation between the pharmacokinetics of the injected cells and the intended pharmacodynamics effect. (biomedcentral.com)
  • The current study represents the first attempt to correlate cell therapy efficacy on OA with the physical presence of the injected haMSCs in the OA model, and demonstrates that human adipose-derived mesenchymal stem cells persisted for 10 weeks locally in the rat joint, coinciding with the efficacy observed. (biomedcentral.com)
  • However, despite having the promising outcome of preclinical studies, the clinical application of stem cell therapy remained elusive due to little or no progress in clinical trials. (hindawi.com)
  • The objective of this study was to provide a generalized critique for the role of mesenchymal stem cell therapy in ischemic stroke injury, its underlying mechanisms, and constraints on its preclinical and clinical applications. (hindawi.com)
  • The recent decade has seen encouraging outcomes of mesenchymal stem cell therapy that holds promise to alleviate the burden of neurological disorders Moreover, initial study data of preclinical trials have also indicated the effectiveness, tolerance, and safety of MSC-based therapy [ 10 ]. (hindawi.com)
  • Herein, we presented an overview of a previously published work regarding the role of stem cell therapy in ischemic stroke and its underlying molecular mechanisms. (hindawi.com)
  • We aim to provide the basis for establishing a future study to promote the clinical translation of stem cell therapy in ischemic brain diseases. (hindawi.com)
  • Ewa K. Stachowiak, PhD, can assist you in areas including in vivo gene transfer and behavioral evaluation of stem cell therapy in animal models. (buffalo.edu)
  • 16. Wang J, Zheng Y, Zhao M. Exosome-Based Cancer Therapy: Implication for Targeting Cancer Stem Cells. (ac.ir)
  • In summary, our study highlights the potential benefits of using the PuroR gene as a selection marker to enhance the purity and efficacy of therapeutic cells in MSC-based gene therapy. (ijstemcell.com)
  • This in vivo pilot study aimed to evaluate the effects of the human dental pulp stem cells conditioned medium on the dental pulp tissue response to vital pulp therapy . (bvsalud.org)
  • Eliminating ROS elimination or inhibiting AKT blocked the effects of the laser therapy in vivo and in vitro. (google.com)
  • These cells are not killed by conventional cancer therapy and thus it is critical to identify those cancer stem cells to be able to improved diagnostics and treatment of cancer. (lu.se)
  • By generating recombinant antibodies against a set of such markers, validating their clinical use, and providing in vivo proof-of-concept data in preclinical models for a targeted antibody-based therapy, novel diagnostic and therapeutic modalities for AML will be designed. (lu.se)
  • Cell Therapy for Parkinsons Disease: What Next? (lu.se)
  • As we have a strong focus on developing cells for cell replacement therapy in Parkinson's disease, we are currently adapting our protocols for GMP compliance through the EU-funded network NeuroStemcellRepair. (lu.se)
Regeneration8
  • For one, if pluripotent stem cells are introduced in numbers large enough for tissue regeneration, there is a high risk of teratomas in an immunodeficient mouse (a standard research model for studying engraftment). (nature.com)
  • These studies will provide insights into the regulatory mechanisms controlling the maintenance, repair and regeneration of tissues, the basis for the development of effective therapeutic applications of stem cells in regenerative medicine. (stemcells-live.fr)
  • On the one hand, this (sterile) neuroinflammation eliminates degenerating tissue, thus supporting a restorative milieu for neuroprotection, axonal regeneration, remyelination, and stem cell-mediated tissue repair. (biomedcentral.com)
  • Effect of human dental pulp stem cell conditioned medium in the dentin-pulp complex regeneration: A pilot in vivo study. (bvsalud.org)
  • The association of dental pulp stem cell conditioned medium with MTA showed beneficial effects on dentin -pulp complex regeneration and has promising potential for studies in regenerative dentistry . (bvsalud.org)
  • Flies have the potential to provide many insights into how to promote the regeneration of sensory hearing cells," said Bonaguidi, who is an assistant professor of stem cell biology and regenerative medicine at the Keck School of Medicine of USC. (eurekalert.org)
  • Over the last years, dentistry has been exploring the potential application of stem cells from different origins in the regeneration of oral tissues that were lost or damaged by disease or trauma 1 . (bvsalud.org)
  • By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)
Mesenchymal stem cell-1
  • Recently, a growing number of studies are focusing on mesenchymal stem cell-based therapies for neurodegenerative disorders. (hindawi.com)
Progenitor4
  • Based on the extensive evidence linking the Notch pathway to stem and progenitor cells in a variety of tissues, we have chosen to mark these cells by the expression of the four Notch receptors. (stemcells-live.fr)
  • This turned out to be the case for both cord blood derived and adult bone marrow stem cells, with LSD1 inhibition expanding stem and progenitor cells from different sources and different stages of development. (lu.se)
  • Within the MAD for Cancer Program we aim to clarify the link between kidney cancer and the kidney progenitor cells, and to develop novel biomarkers for kidney cancer based on our understanding of adult kidney progenitor cells. (lu.se)
  • The allogeneic HSCT was the most frequently performed (57.14%) and the most used source of Hematopoietic progenitor cells (HPC) was the peripheral blood (54.29%) and 5.71% of these patients developed the Graft versus Host Disease (GVHD), of which one was affected by acute GVHD and another by chronic GVHD. (bvsalud.org)
Tissue18
  • In vivo engraftment of stem cell-derived cells into damaged or diseased tissue has many challenges. (nature.com)
  • In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
  • Our research is focused on the in vivo study of normal and malignant stem cells during development and tissue homeostasis in vivo in three dynamic epithelial tissues, the intestine, the mammary gland and the prostate. (stemcells-live.fr)
  • These tissues are particularly suited for our studies, because they are harbor highly active stem cells, to ensure extremely rapid and continuous cell renewal in the case of the intestinal epithelium and to guarantee remarkable tissue remodeling upon hormonal stimulation in the case of the mammary gland and the prostate following castration. (stemcells-live.fr)
  • This has represented a key step in the field, since the obtainment of in vitro NSC systems has been very useful in the last years in order to progress toward disclosure of the complex interplay of different extrinsic (signaling pathways) and intrinsic (transcription factors and epigenetics) signals that govern identity and functional properties of brain tissue-specific stem/progenitors [3]. (unitn.it)
  • An allogeneic source of transplantable cells for use in total bilateral disease can be obtained from culture of donated cadaveric corneal tissue. (liverpool.ac.uk)
  • Currently, little is known about the homing in the muscle tissue and the whole body distribution of the MuStem cells following a systemic delivery which are nevertheless essential to determine the safety and efficacy aspect of the therapeutic strategy. (emc-proceedings.com)
  • Microglia-the resident immune cells of the brain-are activated after brain lesions, e.g., cerebral ischemia, and polarize towards a classic "M1" pro-inflammatory or an alternative "M2" anti-inflammatory phenotype following characteristic temporo-spatial patterns, contributing either to secondary tissue damage or to regenerative responses. (biomedcentral.com)
  • In a resting state, microglia are involved in the maintenance of cell homeostasis surveying their microenvironment and communicating with the neuronal tissue via extremely motile processes and protrusions [ 8 ]. (biomedcentral.com)
  • Tissue Cell;72: 101536, 2021 Oct. (bvsalud.org)
  • For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
  • 43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
  • Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades. (researchgate.net)
  • Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • Materials and method: The pulp tissue, obtained from wisdom teeth, was placed in a 6-well plate containing proper culture medium, and stored at 37 °C and 5% CO2 for cell proliferation and plastic adherence. (bvsalud.org)
  • Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
  • Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
Biology5
  • As suggested by stem classical cell biology, we assumed that the number of mutations in the CSC subpopulation should be lower and distinct compared to the differentiated tumor cells with higher proliferation. (nih.gov)
  • To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
  • In parallel, the development of animal models opened new opportunities to study parasite biology in vivo . (wjgnet.com)
  • An insight into the biology of ischemic stroke indicates that a stream of molecular events initiates instantly after the onset of ischemic stroke, such as oxidative stress, increased level of intracellular calcium, excitotoxicity, and inflammation which results in apoptotic or necrotic neuronal cell death [ 12 - 14 ]. (hindawi.com)
  • Cell Biology International , 35 (4), 417-421. (ewha.ac.kr)
Adipose-derived3
  • Human adipose-derived mesenchymal stem cells (haMSCs) have shown efficacy in treating osteoarthritis (OA) both preclinically and clinically via intra-articular (IA) injection. (biomedcentral.com)
  • A specific population of adipose-derived stem cells (ASCs) is expected to be an alternate source of bone marrow-derived mesenchymal stem cells (BMSCs). (jarde.jp)
  • Goat adipose-derived stem cells (gADSCs) were transduced with either Firefly luciferase (Fluc) or Gaussia luciferase (Gluc) reporter genes and injected in isolated goat intervertebral discs (IVD). (amsterdamumc.org)
Engraftment2
  • Our facility analyzes the engraftment of a variety of stem cells in vivo, the effects of genetic modification of stem cells on engraftment and stem cell function, and the behavioral consequences of neural engraftment. (buffalo.edu)
  • To address this need, we use bismuth ferrite harmonic nanoparticles (BFO-NP, 100-120 nm range) as probes for the tracking of MuStem cells to study in vivo the hMuStem cell engraftment. (emc-proceedings.com)
Multipotent2
  • These represent populations of self-renewing multipotent cells able to differentiate into a variety of neuronal and glial cell types in a time- and regionspecific manner throughout developmental stages and that account for a weak regenerative potential in the adult brain [1]. (unitn.it)
  • Prospective identification, isolation by flow cytometry, and in vivo self-renewal of multipotent mammalian neural crest stem cells. (bvsalud.org)
Lund Stem Cell Center4
  • Researchers from Lund Stem Cell Center have identified a novel target for the expansion of human blood stem cells ex vivo and shed light on the mechanism of action of a stem cell promoting molecule, currently in phase II clinical trials. (lu.se)
  • At the end of 2023, researchers at Lund Stem Cell Center received funding amounting to SEK 77.5 million from the Swedish Research Council, the Swedish Cancer Society, and the Swedish Childhood Cancer Fund. (lu.se)
  • At Lund Stem Cell Center, researchers are using innovative approaches to advance the field, offering exciting possibilities for the future. (lu.se)
  • We hope that this new method will foster a culture of proactive health management and shift the paradigm towards preventive care, in addition to paving the way for more effective treatments," says Sandra Linstedt, research group leader at Lund Stem Cell Center. (lu.se)
Endogenous neural stem1
Mouse xenograft1
  • Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)
Tumor cells5
  • In addition, our results imply that CSCs must be a population of cells that can dynamically switch from differentiated tumor cells, and vice versa. (nih.gov)
  • CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
  • 60% of circulating tumor cells. (nature.com)
  • MTT assays was performed to determine anti-tumor effects of hWJMSCs-secretome on both MCF-7 and 4T1 tumor cells in vitro. (ac.ir)
  • In addition, validation of preliminary findings of solute carrier molecules (usually used for drug-delivery) selectively expressed in tumor cells with the aim to develop imaging-led theranostic approaches of personalized treatment will be performed. (lu.se)
Epithelial8
  • By IVCM, specific limbal morphology of epithelial cells, palisade ridges and projections was assessed. (arvojournals.org)
  • Limbal epithelial cells lost a regular mosaic and conjunctival-type epithelium invaded the limbal space. (arvojournals.org)
  • Vaccination with human amniotic epithelial cells confer effective protection in a murine model of Colon adenocarcinoma. (ac.ir)
  • Limbal stem cell deficiency (LSCD) is a disease resulting from the loss or dysfunction of epithelial stem cells, which seriously impairs sight. (liverpool.ac.uk)
  • We performed a controlled multicenter study to examine the feasibility, safety, and efficacy of allogeneic corneal epithelial stem cells in the treatment of bilateral LSCD. (liverpool.ac.uk)
  • Patients were randomized to receive corneal epithelial stem cells cultured on amniotic membrane (AM): investigational medicinal product (IMP) or control AM only. (liverpool.ac.uk)
  • This first randomized control trial of allogeneic corneal epithelial stem cells in severe bilateral LSCD demonstrates the feasibility and safety of this approach. (liverpool.ac.uk)
  • In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)
Phenotype4
  • Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
  • Furthermore, macrophages were shown to contribute to a niche that can support and maintain a breast CSC (BCSC) phenotype through heterotypic CD90/EphA4 signaling between macrophage and tumor cell 14 . (nature.com)
  • Controlling the phenotype of transplanted stem cells is integral to ensuring their therapeutic efficacy. (amrita.edu)
  • In work performed by Division of Translational Cancer Research it is shows that the hypoxic state pushes neuroblastoma and breast cancer towards an immature, stem cell-like phenotype. (lu.se)
Populations3
  • Our ultimate goal is to explore the signals controlling stem cell homeostasis, in order to gain mechanistic insights into organ morphogenesis, but also into the cellular hierarchy of the highly heterogeneous tumor cell populations. (stemcells-live.fr)
  • DESCRIPTION (provided by applicant): The capacity to evaluate the teratoma forming potential of differentiated embryonic stem cell populations represents an urgent need before widespread therapeutic application of these cells is possible. (sbir.gov)
  • [ 2 ] In addition, some tumor types do not appear to adhere to the CSC concept at all [ 3 , 4 ] or may have multiple independent tumor-initiating cell populations. (medscape.com)
Embryonic stem2
  • The mouse blastocyst-derived embryonic stem cell (ES cell) line BLC6 efficiently differentiates into myosin heavy chain-, desmin- and myogenin- positive skeletal muscle cells when cultivated in embryo-like aggregates (embryoid bodies). (uni-luebeck.de)
  • We differentiated several human embryonic stem cell lines into RPE," said Irina Klimanskaya, Senior Scientist at ACT, and first author of the study. (news-medical.net)
Maturation4
  • We aim to identify novel ways to counteract this maturation, pushing stem cells to self-renew and increase in numbers. (lu.se)
  • Upon maturation, the cell sheets were harvested by lowering the temperature. (researchgate.net)
  • Using whole‐cell patch‐clamp recordings, we characterised the maturation of the grafted cells into functional GABAergic inter-neurons in the host brain, and we confirmed the presence of functional inhibitory synaptic connections from grafted cells onto the host neurons. (lu.se)
  • This includes long-term survival of transplanted cells in vivo to assess cell fate, maturation and integration, as well as functional behavioural assessment for graft function in vivo. (lu.se)
Fate3
  • To achieve this goal, we combine the analysis of Notch-specific cell lineages with functional studies to determine and manipulate the fate of cells associated with Notch activity in both normal and cancer tissues. (stemcells-live.fr)
  • however, this potential is limited by our ability to longitudinally monitor the fate and action of cells used in regenerative therapies. (amsterdamumc.org)
  • Hypoxia is a known regulator of stem cell fate, the effects of which can be mimicked using hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors such as dimethyloxalylglycine (DMOG). (amrita.edu)
CSCs9
  • it is unclear whether the tumorigenicity of CSCs in vivo is solely a proxy for a certain genotype. (nih.gov)
  • The data presented here exclude the possibility that CSCs are only a phenotypic consequence of certain somatic mutations, that is a distinct and non-reversible population of cells. (nih.gov)
  • Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
  • At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
  • Thus, a better understanding of the in vivo mechanisms that regulate CSCs is essential for the development of more effective anti-cancer therapies. (nature.com)
  • Cancer stem cells (CSCs) are thought to the underlying reason for cancer initiation, metastasis, and relapse. (springer.com)
  • Cancer stem cells (CSCs) represent a small subset of the tumor population and reside at the apex of the hierarchy [ 9 ]. (springer.com)
  • Apart from CSCs, there are a myriad of factors and interactions between various cell types and the tumor microenvironment (TME) that ultimately affect CCA progression. (springer.com)
  • SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)
Development in vivo3
  • myogenin, myoD, and myf6 are expressed in these embryoid bodies in a characteristic temporal pattern which precisely reflects the sequence observed during mouse development in vivo. (uni-luebeck.de)
  • Our results suggest that ES cells recapitulate closely the early steps of muscle development in vivo and may serve as an excellent in vitro system to study this process. (uni-luebeck.de)
  • b) Computer-controlled application of a vacuum uniaxially strains the membranes, driving the loading pins apart to stretch the growth channel, and apply controlled dynamic strain to developing scaffold-free tendon fibers, emulating tensile strains seen in tendon development in vivo [90]. (researchgate.net)
Clinical8
  • There appears to be a strong relationship of the microscopic appearance of the limbal palisades of Vogt in aniridia and the corresponding clinical degree of conjunctivalization of the cornea, which lends support to the theory that the palisades of Vogt represents a stem cell niche. (arvojournals.org)
  • Each of those cell sources has specific advantages and disadvantages, and each has found particular clinical applications. (medscape.com)
  • This study aims to explore methodology and to understand in vivo biodistribution of clinical-grade haMSCs labeled with fluorescent dye and injected into an immunocompetent OA rat model. (biomedcentral.com)
  • They compared LSD1 inhibition to treatment with UM171, a human hematopoietic stem cell promoting molecule identified in 2014 and currently in phase II clinical trials, despite an as yet unidentified mechanism of action. (lu.se)
  • Multi-modal imaging techniques that can track cell therapeutics may allow facilitation and optimization of clinical translation therapeutic outcome. (iwmpi.org)
  • In 2011, we showed that a stem cell population (named MuStem cells) isolated from healthy dog skeletal muscle induces long-term muscle repair and striking clinical efficacy after its systemic delivery in the dystrophic dog representing the clinically relevant DMD animal model. (emc-proceedings.com)
  • Investigators at the Department of Clinical Genetics have a strong track record in identifying novel cell surface markers on leukemia stem cells and were the first to demonstrate the potential therapeutic use of recombinant antibodies directed against the marker IL1RAP (interleukin-1 receptor accessory protein) on cancer stem cells in chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). (lu.se)
  • [ 8 ] As such, tryptase is specific to mast cell granules and can provide information about mast cell number, distribution, and activation depending on the clinical context. (medscape.com)
Isolation1
Regenerative medicine3
  • In addition to retinal cells, other stem-cell derivatives are likely to play an important role in the future of regenerative medicine. (news-medical.net)
  • The researchers of the paper from Advanced Cell Technology, collaborated with scientists from the Institute of Regenerative Medicine at Wake Forest University School of Medicine, Winston-Salem, North Carolina, and the University of Chicago, Chicago, Illinios. (news-medical.net)
  • Advanced Cell Technology is a leading biotechnology company in the emerging field of regenerative medicine. (news-medical.net)
Survival2
  • Previous studies have juxtaposed and analyzed immune cell compartments between different CCA categorizations such as short or long-term overall survival (OS) [ 16 ]. (springer.com)
  • [ 16 ] Potential mechanisms through which TCF7L2 variants influence T2DM include its role in adipogenesis, myogenesis, and pancreatic islet development, as well as in beta-cell survival and insulin secretory granule function. (medscape.com)
Proliferation1
Human27
  • In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
  • In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
  • In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
  • Handling, maintenance and understanding of human pluripotent stem cells. (lu.se)
  • Mesenchymal stem cells derived from human adipose tissues favor tumor cell growth in vivo. (ac.ir)
  • Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma. (ac.ir)
  • Human bone marrow mesenchymal stem cells display anti-cancer activity in SCID mice bearing disseminated non-Hodgkin's lymphoma xenografts. (ac.ir)
  • The proto-oncogene SKI is highly expressed in human myeloid leukemia and also in murine hematopoietic stem cells. (edu.au)
  • Restored in vivo-like membrane lipidomics positively influence in vitro features of cultured mesenchymal stromal/stem cells derived from human placenta. (remembrane.com)
  • In Vivo Micro-CT imaging of human mesenchymal stem cells labeled with gold-poly-L-lysine nanocomplexes. (iwmpi.org)
  • Recently, ex vivo three-dimensional organ culture systems have emerged to study the physiology and pathophysiology of human organs. (amsterdamumc.org)
  • Ex vivo molecular rejuvenation improves the therapeutic activity of senescent human cardiac stem cells in a mouse model of myocardial infarction. (uniud.it)
  • We have therefore generated `knock-in' mouse embryonic stem (ES) cells to investigate the effects of expressing a commonly found hot-spot p53 mutant, R246S - the mouse equivalent of human R249S, which is associated with hepatocellular carcinomas. (silverchair.com)
  • The knock-in cells contain higher levels of p53 that localizes to the nucleus even in the absence of genotoxic stress and yet remains non-functional, reminiscent of mutant p53 found in human tumours. (silverchair.com)
  • During last years, our group isolated the human counterparts (hMuStem) and demonstrated that they share the same phenotypic and in vitro behavioral features of canine cells. (emc-proceedings.com)
  • Concentrated conditioned medium was obtained by incubating characterized human dental pulp stem cells with fresh culture medium. (bvsalud.org)
  • Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)
  • A) Colonies of human embryonic stem cells, bright field microscopy, scale bar: 100µm. (gsi.de)
  • D) Karyotype of a human cell after heavy ion exposure revealed by mFISH technique. (gsi.de)
  • Using human pluripotent stem cells, scientists engineered an amniotic sac-like structure, potentially useful for uncovering the elusive cellular signals that lead to infertility. (the-scientist.com)
  • Human embryonic stem cells are so exciting because they provide a pathway of making many desperately-needed cell types for use in medicine," said Michael D. West, President & C.E.O. at ACT and an author on the paper. (news-medical.net)
  • Besides use in the aging retina, human embryonic stem cells have now demonstrated the potential to change the face of several age-related degenerative diseases, perhaps one day allowing physicians to prescribe young cells they way they dispense pills today. (news-medical.net)
  • We generated GABAergic interneuron precursors from human embryonic stem cells (hESCs) and grafted them in the hippocampi of rats developing chronic SRSs after kainic acid‐induced status epilepticus. (lu.se)
  • Exploring the potential of human-derived cell lines and glial cell line-derived neurotrophic factor for network inhibition. (lu.se)
  • To complement the in vivo system of chick embryos, we employ patient omics data, single cell RNA sequencing, human and mouse embryos, human cancer cell cultures, human pluripotent stem cells, chick embryo neural crest-derived in vitro crestosphere cultures, and zebrafish and mouse in vivo models. (lu.se)
  • However, with a growing number of novel cell sources (stem cells and reprogrammed cells) it becomes important to develop new methodologies for a more thorough assessment of human neurons generated using these approaches. (lu.se)
  • We utilise monosynaptic tracing technology, using pseudotyped rabies vectors, in order to visualise the synaptic contacts formed between host neurons and graft human cells. (lu.se)
Limbal stem4
  • Biomarkers of in vivo limbal stem cell function. (ca.gov)
  • The purpose of this study is to evaluate markers in the eye that can predict and help diagnose limbal stem cell deficiency. (ca.gov)
  • A formula that has into account all of these markers was generated to diagnose limbal stem cell deficiency. (ca.gov)
  • PURPOSE: To evaluate in vivo parameters as biomarkers of limbal stem cell function and to establish an objective system that detects and stage limbal stem cell deficiency (LSCD). (ca.gov)
20191
Extracellular vesicles2
  • 10. Rani S, Ryan AE, Griffin MD, Ritter T. Mesenchymal stem cell-derived extracellular vesicles: toward cell-free therapeutic applications. (ac.ir)
  • 3-D cell culture recapitulates in vivo environments for generating physiologically relevant extracellular vesicles. (the-scientist.com)
Flow cytometry2
  • Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)
  • Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
Mechanisms2
  • hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)
  • The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
Metastasis2
  • We found that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. (cdc.gov)
  • Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)
Heterogeneity2
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • The CSC is defined as a cancer cell with self-renewing capacity that can regenerate the original tumor in all its differentiated heterogeneity. (medscape.com)
Molecular4
  • We attempted to reveal the existence of labeled cells in vivo by imaging and a molecular biomarker approach, and to correlate with the in vivo efficacy and physical presence over a follow-up period up to 10 weeks. (biomedcentral.com)
  • Molecular rejuvenation of patient-derived CSC by short pharmacologic conditioning boosts their in vivo reparative abilities. (uniud.it)
  • These cells expressed RPE-specific molecular markers and were capable of phagocytosis, an important RPE function. (news-medical.net)
  • Differentiomics could also be a valuable predictive tool for quality assessment of other ES cell derivatives based on their molecular signature. (news-medical.net)
Neuronal1
  • While expanding our knowledge of graft integration, we also investigate how that occurs at a functional level by utilising optogenetics in combination with highly sensitive and quantitative methods assessing neuronal function, such as electrophysiology and in vivo amperometry. (lu.se)
Myeloid5
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
  • This was first demonstrated in acute myeloid leukemia (AML) but during recent years, cancer stem cells have also been demonstrated in other cancer types. (lu.se)
  • However, prior to commitment, it has been cells, it is evident that GATA-1 and PU.1 are able to specify observed that many genes are expressed at intermediate or basal erythroid and myeloid cell fates (see [16] and references therein). (lu.se)
Autologous1
  • Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
Donor5
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • This approach has the advantages of rapid availability and reduced stringency for a complete donor/recipient match, but is limited by a relatively low number of stem cells within a single cord blood unit. (lu.se)
  • One focus of the Stem Cell Regulators research group, led by Prof. Jonas Larsson, is the development of new strategies to expand stem cells ex vivo , allowing for the treatment of patients who otherwise would lack a suitable donor. (lu.se)
  • A GVHD mouse model was established by transplanting C57BL/6 donor bone marrow cells and C57BL/6 EGFP (enhanced green fluorescent protein) splenocytes into lethally irradiated BALB/c nude recipient mice. (ewha.ac.kr)
  • the donor.4,11 In the case of nerve cells obtained from either delivery of dopamine released from cells the developing central nervous system (CNS), the opti- implanted into the ventricle, adjacent to the stria- mal age was shown to be at, or close to, the cell-cycle tum,6,7 or restoration of synaptic dopamine release exit. (lu.se)
Therapeutic potential1
  • Evaluation and comparison to their in vivo counterparts will be critical for assessment of their therapeutic potential. (news-medical.net)
Tissues1
  • According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)
Characterization1
  • Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
Cancer14
  • Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
  • 90% of cancer-related deaths, is a multi-step process involving cancer cell intravasation into the bloodstream, dissemination to distant sites, and seeding in new organs 1 . (nature.com)
  • We also dissect the involvement of Notch in stem cell homeostasis and in cancer initiation. (stemcells-live.fr)
  • 1. Usha L, Rao G, Christopherson II K, Xu X. Mesenchymal stem cells develop tumor tropism but do not accelerate breast cancer tumorigenesis in a somatic mouse breast cancer model. (ac.ir)
  • 4. Yang J, Lv K, Sun J, Guan J. Anti-tumor effects of engineered mesenchymal stem cells in colon cancer model. (ac.ir)
  • 9. Fatima F, Nawaz M. Stem cell-derived exosomes: roles in stromal remodeling, tumor progression, and cancer immunotherapy. (ac.ir)
  • However, due to their elusive character and differences in identification among different types of cancer, it remains a challenge to study such cells. (springer.com)
  • Here, we employ a fluorescent reporter system to track and isolate stem-like cancer cells of cholangiocarcinoma cell lines. (springer.com)
  • SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)
  • 3-D balls of cells can be used to screen for potential cancer drugs. (the-scientist.com)
  • The project funded by Cancerfonden, will focus on using exhaled breath particles as a non-invasive method for diagnosing and monitoring non-small-cell lung cancer. (lu.se)
  • Within the MAD for Cancer Program, a previously unexplored combination of strategies will be used to identify new cell surface markers on AML stem cells. (lu.se)
  • The strategy used to identify new therapeutic markers on leukemia stem cells will also be explored in other tumor types studied within the MAD for Cancer Program. (lu.se)
  • Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)
Self-renewal1
  • SCs are not only capable of maintaining themselves (self-renewal) but also give rise to a proliferative 'transit amplifying' compartment and ultimately to postmitotic differentiated cells with specialized functions. (medscape.com)
Hematopoietic stem8
  • In steady-state adult hematopoiesis, most hematopoietic stem cells (HSCs) are in the resting phase of the cell cycle. (uzh.ch)
  • When grown outside the body hematopoietic stem cells proliferate and differentiate from an immature state to more mature blood cell types. (lu.se)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • Collectively our results demonstrate that SKI is an important regulator of hematopoietic stem cell activity and its overexpression leads to myeloproliferative disease. (edu.au)
  • 00:39 police is these are hematopoietic stem cells. (yale.edu)
  • The overall death infections that occurred in 2 clusters of hematopoietic stem rate was 50%, and death occurred within 8 days after diag- cell transplant (HSCT) recipients in our institution prompt- nostic culture collection. (cdc.gov)
  • Hematopoietic stem cell lineage choices are decided by genetic networks that are turned ON/OFF in a switch-like manner. (lu.se)
  • It levels for the hematopoietic stem cell system [1,2]. (lu.se)
Neurons7
  • image: In the fly, the sensory hearing cells are neurons located in a segment of their antennae known as the Johnston's Organ, or JO. (eurekalert.org)
  • Instead, they observed JO neurons proliferate and produce more of themselves--although this doesn't rule out the potential for other types of cells to also produce JO neurons. (eurekalert.org)
  • In flies given the chemotherapy drug cisplatin, which is known to kill sensory hearing cells, JO neurons compensated for the damage by proliferating even more. (eurekalert.org)
  • The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
  • VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)
  • The cells are called induced neurons (iNs) and offer a shortcut over induced pluripotent stem cells (iPSc) for generating patient and disease-specific neurons. (lu.se)
  • Current efforts focus on identifying the optimal starting cell population for neural conversion, identification of gene combinations that yield subtype specificity and analysis of function and integration of the reprogrammed neurons. (lu.se)
Correlates1
  • This correlates to metastatic spread and aggressive disease in vivo. (lu.se)
NSCs2
  • During neural development, a relatively small and formerly considered homogeneous population of Neural Stem cells (NSCs) gives rise to the extraordinary complexity proper of the Central Nervous System (CNS). (unitn.it)
  • Here we will review the functional properties of different in vitro NSC systems, providing also a direct comparison with NSCs present in vivo. (unitn.it)