Vivo retroviral transduction. Medical search. Frequent questions

Using retroviral vectors foreign DNS ex vivo gentransfer was accomplished. (tum.de)
however, the transduction properties of Ad vectors in neonates and infants have not been fully examined. (hindawi.com)
On the other hand, Ad vectors also have a number of disadvantages, including their low transduction efficiency in cells lacking coxsackievirus-adenovirus receptor (CAR) and their induction of innate immune responses. (hindawi.com)
These vectors can be used both for direct injection (in vivo gene transfer) or transduction of cells in culture for subsequent transplantation (ex vivo gene transfer). (lu.se)
To address the problem of sustained expression in vivo (a major limitation of many gene transfer methods as of now) we are investigating the use of different promoters, post-transcriptional regulating elements and vectors. (lu.se)
By design, our lentiviral vectors lack the genes required for viral packaging and transduction (these genes are instead carried by helper plasmids used during virus packaging). (vectorbuilder.kr)
We will first focus on ex vivo applications and then go through some examples of in vivo administrations of the targeted vectors. (hstalks.com)
By in vivo, we mean that vectors are directly injected into the patient, which can be locally, such as intracerebrally, or systemically. (hstalks.com)
It is obvious that upon in vivo applications, vectors will encounter a large variety of cell types besides the actual target cells relevant for therapy. (hstalks.com)
To understand the strategies to achieve surface targeting, we first have to look at the entry routes of retroviral vectors. (hstalks.com)
Retroviral vectors are envelope particles that bind with their surface receptor for cell entry. (hstalks.com)
Compared to adenovirus and AAV, retrovirus-based vectors show relatively low transduction efficiency in-vivo , but their advantage lies in their ability to integrate stably and confer long-term transgene expression in dividing tissues. (biopharminternational.com)
2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)

We have tested the functionality of RQR8 in vitro and in vivo as well as in combination with T-cell engineering components. (ashpublications.org)
Therefore, the aim of our study was to develop a transplantable immunocompetent mouse model of HPV-positive OSCC and characterize it in vitro and in vivo. (biomedcentral.com)
Transduction of naive GTL-16 cells with truncated, but not full length, RAF1 and BRAF conferred in vitro and in vivo resistance to MET inhibitors, which could be reversed by MEK inhibition. (unito.it)
The efficient delivery of functional therapeutic genes into target cells in vitro and in vivo is an important problem in gene therapy approaches for the treatment of hereditary diseases and cancer (9, 10). (ualberta.ca)
Chitosan microparticles containing reporter genes are being used for the transfection of mammalian cells both in vitro and in vivo conditions (28-30). (ualberta.ca)

We are currently working with three different vector systems: adeno-associated viral, retroviral and lentiviral. (lu.se)
Retroviral and lentiviral pseudotypes have been patients. (cdc.gov)
For instance, Agilent has retroviral and lentiviral vector systems that are pseudotyped to achieve a wide target range. (biopharminternational.com)

Transfection efficiency and consistency were significantly higher than those following retroviral transduction (87% ± 6 vs. 60% ± 20). (maxcyte.com)
Adjuvants for ex vivo therapy settings need to increase transduction efficiency while being easily removed or degraded post-transduction to prevent the risk of venous embolism after infusing the transduced cells back to the bloodstream of patients, yet no such peptide system have been reported thus far. (bvsalud.org)
In this study, we introduce peptide amphiphiles (PAs) with a hydrophobic fatty acid and a hydrophilic peptide moiety that reveal enhanced viral transduction efficiency. (bvsalud.org)
Our findings establish PAs as additives in retroviral gene transfer, rivalling commercially available transduction enhancers in efficiency and degradability with promising translational options in clinical gene therapy applications. (bvsalud.org)
Alternate codon usage significantly enhanced the translational efficiency of this tightly regulated gene in retroviral vector-transduced cells. (johnshopkins.edu)
In the present study, we have investigated DNA encapsulation efficiency and loading capacity of chitosan microparticles prepared in different conditions, as well as in vitro DNA release from microparticles, and transfection of different cell lines with chitosan-DNA microparticles, which may be employed in future in vivo studies. (ualberta.ca)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
This retrovirus-based style of VEGF-driven angiogenesis offers the advantage of high retroviral transduction efficiency that is favored in proliferating cells and ECs are actively dividing in response to continuous VEGF-stimulation [15] [16]. (ecologicalsgardens.com)

This system is optimized for high copy number replication in E. coli, high-titer packaging of live virus, efficient viral transduction of a wide range of cells, and efficient vector integration into the host genome. (vectorbuilder.kr)

In vivo anti-tumor activity was demonstrated using a mouse model of acute lymphoblastic leukemia (ALL). (maxcyte.com)
These data imply that Smac mimetics can cooperate with TNFα secreted by tumor-associated immune cells to kill osteosarcoma cells in vivo. (biomedcentral.com)
Retroviral transduction of tumor antigen-specific T-cell receptor (TCR) genes into lymphocytes redirects T cells to lyse tumors. (johnshopkins.edu)
In addition, tumor models were characterized in vivo in C57Bl/6NCrl mice in terms of their histological properties, tumor growth kinetics, and radiosensitivity. (biomedcentral.com)
In vivo, cisplatin-induced immunogenic cell stress synergistically potentiated CTL-mediated tumor control in a human model of HPV-associated malignancy. (bmj.com)

Accordingly, this study examined the properties of Ad vector-mediated transduction in neonatal mice. (hindawi.com)
The retroviral vector pGCDNsam was employed for gene transduction into fetal hepatoblasts and human iPSC-derived hepatoblasts23. (nicotinic-receptor.com)
In this report we describe the generation of a retroviral vector encoding a codon-optimized IL-15 gene. (johnshopkins.edu)
Activated human CD4 + and CD8 + human lymphocytes expressed IL-15Rα and produced high levels of cytokine upon retroviral transduction with the IL-15 vector. (johnshopkins.edu)
The various retroviral product packaging cells portrayed retrovirus encoding the validated dominant-negative (DN) mutant of calpain-I [8] outrageous type (WT) calpain-I [8] or no put in Rplp1 (clear vector). (ecologicalsgardens.com)

Stampidine epigenetically modulates the host transcriptome in a unique manner, silences expression of a distinct set of genes encoding transcription factors and signal transduction molecules, and prevents HIV infection from distorting and disrupting key cellular transcriptional networks. (wikipedia.org)
Marker genes enable measurement of transduction and allow selection of transduced cells, whereas suicide genes allow selective deletion of administered T cells in the face of toxicity. (ashpublications.org)
Datasets have been normalized, and transcription-related genes with differential expression during in vivo liver development were extracted and represented as a heat map. (nicotinic-receptor.com)

As well as the VEGF-expressing cells we included similar amounts of retroviral product packaging cells within the Matrigel for constant delivery of built retroviruses. (ecologicalsgardens.com)
Retroviruses and lentiviruses, therefore, are most suitable for ex-vivo gene transfer, Chaudhary states. (biopharminternational.com)

The contribution of CXCL12 to hypoxia-induced angiogenesis was examined in vivo using a subcutaneous murine model of neovascularization. (haematologica.org)
To characterize the transforming ability of leukemic oncogenes, researchers in the field have developed an ex vivo murine myeloid transformation assay using retroviral gene transduction and its protocol has been improved over the past 10 years. (bio-thing.com)
To better understand the mechanism of disease onset, several murine leukemia models using retroviral gene transduction, xenograft, or Cre-mediated chromosomal translocation have been developed over the past twenty years. (bio-thing.com)
Particularly, a retroviral gene transduction-mediated murine leukemia model has been frequently used in the leukemia research field. (bio-thing.com)
We identify specific DAP12-associated receptors expressed by osteoclasts and examine function of DAP12 in murine osteoclasts in vivo and in vitro. (elsevierpure.com)

In vivo assays (mouse genetics, intravenous and intrafemoral transplantation assays, subcutaneous pump implantation for delivery of candidate therapies, humanized mouse models, pre-clinical testing of immunotherapies in xenotransplantation models, bioluminescence imaging). (postdocjobs.com)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)

The use of immunomodulatory adjuvants, eg, vaccination or cytokine therapy, for in vivo T-cell activation may help overcome this metabolic quiescence and thus augment cellular immunotherapy-based cancer therapy. (johnshopkins.edu)

Generation of retrovirus for gene transduction. (nicotinic-receptor.com)

In Vivo targeted molecular magnetic resonance imaging of free radicals in diabetic cardiomyopathy within mice. (omrf.org)

BIOL 6341 Oncogenes (3 semester credit hours) Properties of cancer cells, in vivo and in vitro. (utdallas.edu)
RESULTS- Lcn2 is highly expressed by fat cells in vivo and in vitro. (diabetesjournals.org)

Over-expression of hypoxia-inducible factor in multiple myeloma plasma cells strongly induced in vivo angiogenesis, and administration of a CXCL12 antagonist decreased hypoxia-inducible factor-induced angiogenesis. (haematologica.org)
A Dominant-negative Mutant of Calpain-I Improves VEGF Neovessel Integration and Lumen Development In Vivo To research a possible romantic relationship between calpain activity and abnormal neovascular structures we employed a recognised mouse style of VEGF-driven angiogenesis [15] [16]. (ecologicalsgardens.com)

Eighteen such patients were analyzed for persistence and gene expression in vivo. (johnshopkins.edu)
Although gene-modified lymphocytes persisted in vivo, the shutdown of TCR transgene expression was observed. (johnshopkins.edu)
Bisulfite sequencing analysis and ex vivo DNA methyltransferase inhibition demonstrated that the decrease in gene expression did not result from DNA methylation. (johnshopkins.edu)
These data demonstrate a lack of γ-retroviral promoter-specific gene silencing in adoptively transferred human lymphocytes and support that transgene expression is largely affected by global cellular mechanisms. (johnshopkins.edu)
Two monoclonal HPV-positive OSCC mouse cell lines were established by inducing the expression of HPV-16 oncogenes E6 and E7 in the MOC1 OSCC cell line using retroviral transduction. (biomedcentral.com)
Cells were transduced with three different retroviral cDNA expression libraries and selected for growth in the presence of the MET inhibitor PHA-665752. (unito.it)
Restoration of DAP12 expression by retroviral transduction of DAP12 -/- osteoclast precursors rescued in vitro osteoclast multinucleation, Direct sfimulation of DAP12 expressed in RAW264.7 during in vitro osteoclastogenesis led to a marked increase in the number of TRACP + multinucleated osteoclast-like cells formed. (elsevierpure.com)
Lentivirus can effectively transduce both dividing and non-dividing mammalian cells, and integrate into the host genome, allowing stable long-term, high-level gene expression both in vivo and in vitro. (onenucleus.com)

Experience performing in vivo procedures in mice. (postdocjobs.com)

Michael Koch, a Ph.D. student within the BMLS research group said "As epithelial organoids have a stem cell-like character, which makes them difficult to transduce, they require optimised transduction protocols. (onenucleus.com)

In the setting of adoptive cell transfer, IL-15-transduced lymphocytes may prolong lymphocyte survival in vivo and could potentially enhance antitumor activity. (johnshopkins.edu)

The role of oncogenes in growth hormone signal transduction. (utdallas.edu)

In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)

by "transduction," we mean the introduction of genetic material into target cells. (frontiersin.org)
The CAR-T cell manufacturing platform is based on the ex vivo genetic engineering of a patient's T cells to express CAR, followed by the reinfusion of the T cells into the patient. (biomedcentral.com)

In ex-vivo , cells are modified outside the patient's body and the corrected version is transplanted back into the patient. (biopharminternational.com)
Opposite of ex-vivo is what we call in-vivo , where cells are treated inside the patient's body. (biopharminternational.com)

Methods Retroviral transduction with HPV16 E7 established a novel HPV-associated sinonasal squamous cell carcinoma (SNSCC) cell line. (bmj.com)

However, they have some disadvantages in their usage in vivo such as immunogenicity, insertional mutations, and potential pathogenicity (20). (ualberta.ca)

In vivo, 68 Ga-BMS-986192 uptake was high at 1 h after injection in PD-L1-positive tumors (9.0 ± 2.1 percentage injected dose [%ID]/g) and kidneys (56.9 ± 9.2 %ID/g), with negligible uptake in other tissues. (snmjournals.org)

As an alternative approach, growth factor receptors or components of their signal transduction machinery may be targeted directly. (springer.com)
The binding of specific T cell receptors (TCR) to major histocompatibility complex (MHC)/antigen complexes on cancer cells induces the TCR/CD3-mediated signal transduction pathway and stimulates the release of specific toxins (perforin and granzyme) for cancer elimination [ 6 , 7 ]. (biomedcentral.com)

Retroviral SARS-CoV S capsid (N) proteins (10). (cdc.gov)

However, inefficient virion-cell attachment resulting in low gene transduction efficacy remains a major challenge in clinical applications. (bvsalud.org)

The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)

iii) Neonates and infants have higher stem/progenitor cell ratios, and thus transduction into stem/progenitor cells may occur more efficiently than in adults. (hindawi.com)
Although the Smac mimetics were effective in vivo, in vitro they only efficiently killed osteosarcoma cells when TNFα was supplied. (biomedcentral.com)

In vitro observations were translated using an in vivo xenograft NSG mouse model of human cervical carcinoma evaluating cisplatin in combination with CTL adoptive cell transfer. (bmj.com)
Unlike traditional retroviral system, the AMSBIO lentivirus is much more actively imported into the nuclei of non-dividing cells and stably integrated into the host cell's genome independent of cell cycle. (onenucleus.com)

In keeping with these observations co-culture of the many retroviral product packaging cells using the VEGF-expressing SK-MEL-2 cells within the same proportions used in vivo acquired no influence on VEGF creation (see Strategies) needlessly to say because VEGF appearance in this technique is constitutively powered by way of a CMV promoter. (ecologicalsgardens.com)

Retroviral gene delivery is the key technique for in vitro and ex vivo gene therapy. (bvsalud.org)
Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. (frontiersin.org)
In gene therapy, we do basically distinguish between in vivo and ex vivo modification of target cells. (hstalks.com)
Generally, AAV and adenoviruses are primarily used for creating in-vivo gene therapy. (biopharminternational.com)

To assess effects on insulin action, we used retroviral delivery of short hairpin RNA to reduce Lcn2 levels in 3T3-L1 adipocytes. (diabetesjournals.org)

Ex vivo means that target cells are removed from the patient then transduced ex vivo in culture vessels before they will be re-implanted into the patient. (hstalks.com)

Anatomy15

Organisms15

Diseases2

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes25

Information Science3

Vectors13

Vitro and in vivo5

Lentiviral3

Efficiency9

Viral transduction1

Tumor5

Vector5

Genes3

Retroviruses2

Murine5

Transplantation2

Cytokine1

Retrovirus1

Molecular1

Cells in vivo2

Angiogenesis2

Expression8

Mice1

Transduce1

Lymphocytes1

Oncogenes1

Hematopoietic2

Genetic2

Patient's2

Methods1

Disadvantages1

High1

Signal2

Proteins1

Clinical1

Activity1

Efficiently2

Cell2

Observations1

Therapy4

Levels1

Target cells1