• Recently, tau has been shown to undergo liquid liquid phase separation (LLPS) both in vivo and in vitro . (biorxiv.org)
  • This study examined the effects of Geranium sibiricum L. extract (GSE) on hair growth, using in vitro and in vivo models. (biomedcentral.com)
  • These results demonstrated that GSE promotes hair growth in vitro and in vivo by regulating growth factors and the cellular response. (biomedcentral.com)
  • Further studies indicated that the nanoprobe exhibits excellent tumor-targeting imaging ability in vitro and in vivo by effectively recognize the over-expressed nucleolin (NCL) on the breast cancer cells membrane. (biomedcentral.com)
  • Rhodiola crenulata root extract (RCE) has been shown to possess protective activities against hypoxia both in vitro and in vivo. (mdpi.com)
  • For instance, PLA could be combined with PGA to form poly(lactic- co -glycolic acid) (PLGA), which has degradation rate tailored with the tissue healing period and has been shown to support osteoblast cells attachment and growth in vitro and in vivo [ 22 - 24 ]. (hindawi.com)
  • Hochmair J, Exner C, Betzel C, Mandelkow E , Wegmann S . Light Microscopy and Dynamic Light Scattering to Study Liquid-Liquid Phase Separation of Tau Proteins In Vitro. (neurotree.org)
  • The results could potentially fill the translational gaps of in vitro/in vivo or preclinical species to human extrapolation of hepatobiliary elimination mediated by MRP2/Mrp2. (aspetjournals.org)
  • the in vitro or in vivo model for biliary excretion predication is far from being mature ( Mahmood and Sahajwalla, 2002 ). (aspetjournals.org)
  • New tracers that can deliver 19F to amyloid plaques or detect metabolic brain disorder associated with ND are designed, synthesized ans tested in vitro and in vivo. (cnr.it)
  • N protein can also undergo liquid-liquid phase separation (LLPS) with viral genome RNA and inhibit stress granules to facilitate viral replication and assembly. (ijbs.com)
  • We report on a model system of hydrophobically driven LLPS induced by high salt concentration (LLPS-HS), and compare it to electrostatically driven LLPS represented by tau-RNA/heparin complex coacervation (LLPS-ED). We show that LLPS-HS promotes tau protein dehydration, undergoes maturation and directly leads to canonical tau fibrils, while LLPS-ED is reversible, remains hydrated and does not promote amyloid aggregation. (biorxiv.org)
  • Biomacromolecules can form biomolecular condensates through liquid-liquid phase separation (LLPS, referred to as phase separation ) mediated by multivalent weak interactions. (medicaltrend.org)
  • This pore-forming toxin has been reported as an important protein that mediates tissue damage promoted by S. aureus . (nature.com)
  • As researchers and higher education providers, we want to design new strategies for the several aims listed below, trying to promote the application of nano, polymeric or protein-based materials in chemical biology and medicine, at the same time training new generations of scientists with good skill, innovation and knowledge in this area. (baigroup.online)
  • Actin-related protein 2/3 (Arp2/3) complex activation by nucleation promoting factors (NPFs) such as WASP, plays an important role in many actin-mediated cellular processes. (elifesciences.org)
  • In the nuclei of VU cells, residual NHR-67 protein is compartmentalized into discrete punctae that are dynamic over the cell cycle and exhibit liquid-like properties. (elifesciences.org)
  • Besides the function in viral life cycle, N protein can bind GSDMD to antagonize pyroptosis but promotes cell death via the Smad3-dependent G1 cell cycle arrest mechanism. (ijbs.com)
  • S100B is a calcium/zinc-binding protein secreted by mature astrocytes, associated, at high levels, with nervous tissue injury in a variety of neuropathological processes, including Multiple Sclerosis in the acute phase. (cnr.it)
  • These findings suggest that EphA2 is a novel membrane protein with phase separation ability and is associated with ferroptosis and immune cell infiltration, which further suggests that malignant progression of colorectal cancer may be inhibited by suppressing the phase separation ability of EphA2. (aging-us.com)
  • We also found that EphA2 can form liquid-liquid phase separation condensates on cell membrane, which can be disrupted by ALW-II-41-27, an inhibitor of EphA2. (aging-us.com)
  • icFSP1 exhibits a unique mechanism of action, which promotes ferroptosis in cancer cells by triggering the phase separation of FSP1, thereby inhibiting tumor growth. (medicaltrend.org)
  • In order to develop effective FSP1 inhibitors in vivo, the research team screened about 10,000 small molecules and conducted DMPK (Drug Metabolism and Pharmacokinetics) verification studies, and finally discovered and determined a new in vivo effective The FSP1 inhibitor, icFSP1 , sensitizes many human cancer cells to ferroptosis and inhibits tumor growth in vivo. (medicaltrend.org)
  • icFSP1 can strongly inhibit tumor growth in vivo, and there is obvious formation of FSP1 aggregates in tumor tissue. (medicaltrend.org)
  • In vivo, D4A is more potent than Abi in inhibiting tumor growth in a mouse xenograft model. (aacc.org)
  • It also promotes extracellular matrix (ECM) deposition and permits transportation for nutrient and gases and waste removal for cell survival [ 2 ]. (hindawi.com)
  • The NEXUS project will develop an easily customizable integrated platform to enable Extracellular Vesicles (EVs) separation, purification, and multivariate characterization from complex biofluids. (cnr.it)
  • These studies provide evidence that WASP and WIP play central roles in establishment of a robust multivalent SH3 domain-PRM network in vivo, giving actin assembly onset at endocytic sites a switch-like behavior. (elifesciences.org)
  • Pharmacokinetic studies and tissue distributions were performed and detected by an ultra-high performance liquid chromatography/mass spectrometry system. (dovepress.com)
  • By using our newly developed liquid chromatography-tandem mass spectrometry quantification method, we were able to measure the absolute amount of MRP2/Mrp2 in liver tissues and isolated hepatocytes across species. (aspetjournals.org)
  • 1986. Movement of selected organic liquids into dry soils. (cdc.gov)
  • In HIV infected cells, knockout of FUS delays the gradual entry of HIV into latency, and similarly promotes viral activation in a T cell latency model that is treated with JQ1. (biomedcentral.com)
  • These findings suggest that icFSP1 does not directly inhibit the activity of the FSP1 enzyme, but instead triggers phase separation of FSP1. (medicaltrend.org)
  • In October 2019, the Marcus Conrad team of Helmholtz Munich Research Center in Germany published a paper in Nature [1] , which identified FSP1 as a new type of potent ferroptosis inhibitor independent of glutathione , through the production of coenzyme Q10 Antioxidant forms of (CoQ) promote cancer cell resistance to ferroptosis. (medicaltrend.org)
  • The study found that phase separation of FSP1 promotes ferroptosis and led to the development of a new anticancer drug called icFSP1, which sensitizes cancer cells to ferroptosis. (medicaltrend.org)
  • This study revealed for the first time the link between ferroptosis and phase separation, and based on this, a new concept of cancer therapy by promoting FSP1 phase separation and ferroptosis was proposed. (medicaltrend.org)
  • A tumor-targeting nanoprobe based on the functional and programmable Liquid-Liquid phase separation of AS1411 promoted by Ru(II) complex RuPEP may develop into a potential phosphorescence probe to detect breast cancer cells, where AS1411 act as a tumor-targeting guidance moiety to distinguish tumor cells from normal cells and RuPEP act as a light-emitting element to highlight breast cancer cells. (biomedcentral.com)
  • This study produce a potent nanoprobe can be used as a convenient tool to highlight and distinguish tumor cells in vivo, and indicate the tumorous grading and staging in human breast cancer patient pathological section, which provides an effective way for breast cancer diagnostic imaging by targeting recognize NCL. (biomedcentral.com)
  • Researchers have been struggling to understand if different forms, or strains, of tau distinguish different tauopathies or different phases of disease progression. (alzforum.org)
  • In yeast, a member of the WASP family of proteins promotes the assembly of actin filaments around the site where endocytosis will occur. (elifesciences.org)
  • Cytokinetic bridge triggers de novo lumen formation in vivo. (syracuse.edu)
  • As it is known that the sensitivity of metal centers and the resulting low activity and turnover of such artificial metalloenzymes in vivo are systematic problems not fully solved, we are actively seeking for potential solutions to this problem by creating microenvironments inside cells, so that artificial metalloenzymes can enjoy benign media in which they can safely do their catalysis job. (baigroup.online)
  • Specific recent and current research topics that contribute to the synthetic biology toolbox for development of artificial cells include membrane texturing through phase separation, artificial DNA adhesion receptors, hybrid lipid-polymer membrane systems for enhanced stability and lifetime, encapsulation of feedback-responsive systems and controlled generation of eukaryote-like membrane architectures. (royalsociety.org)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
  • It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
  • Zhang and Ma have developed [ 25 ] a highly porous biodegradable polymer/apatite composite scaffold (95% porosity) through a thermally induced phase separation technique, which resulted in significant improvement in mechanical properties compared to polymer-only scaffold. (hindawi.com)
  • In addition, both the pristine polymer and the dispersion exhibited an interfacial Maxwell-Wagner relaxation, likely due to spatial heterogeneities associated with phase disproportionation in this polymer. (ibecbarcelona.eu)
  • MIP Technologies develops and produces molecularly imprinted polymers (MIPs) and other `smart' polymer materials for use in trace analysis or in advanced separations applications. (lu.se)
  • ja":"C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. (tokushima-u.ac.jp)
  • The endocytic pathway is the gateway between cells and their environment, and promotes diverse events such as nutrient uptake and cell migration. (royalsociety.org)
  • Single-molecule spectroscopy can be an effective tool to probe heterogeneity because molecular species can be observed one at a time, so individual oligomers can be detected without separation. (biorxiv.org)
  • The mechanism of transport through porins is of utmost and direct interest for drug discovery, extending toward nanotechnology applications for blue energy, separations, and sequencing. (ibecbarcelona.eu)
  • Xenobiotics and their metabolites are generally eliminated and detoxified by phase I and phase II enzymatic metabolism, by phase III transporter-mediated drug efflux to bile, or by both mechanisms. (aspetjournals.org)
  • To avoid these problems, we developed a method that allowed us to separate the metabolites using a conventional reverse-phase C18 column with a mobile phase consisting of methanol, acetonitrile, and formic acid. (aacc.org)
  • Development and validation of a novel LC-MS/MS method for simultaneous determination of abiraterone and its seven steroidal metabolites in human serum: Innovation in separation of diastereoisomers without use of a chiral column. (aacc.org)
  • Hair-growth promoting effect was measured in animal model. (biomedcentral.com)
  • Topical application of 1,000 ppm GSE for 3 weeks promoted more significant hair growth on shaved C57BL/6 mice than did 5% minoxidil. (biomedcentral.com)
  • Among the natural extracts, Allium cepa extract and Ziziphus jujuba extract were found to promote hair growth [ 5 , 6 ], with the antioxidant capacity of each extract being concluded as the contributing factor. (biomedcentral.com)
  • Additionally, histological analysis of the growth plate of PKAchu mice demonstrated that CNP increased the length of the growth plate, but coadministration of a PKA inhibitor, H89, inhibited the growth-promoting effect of CNP only in the hypertrophic zone. (tokushima-u.ac.jp)
  • In summary, we revealed that CNP-induced cGMP elevation activated the cAMP/PKA pathway, and clarified that this PKA activation contributed to the bone growth-promoting effect of CNP in hypertrophic chondrocytes. (tokushima-u.ac.jp)
  • Pluripotent cardiac progenitor cells resident in myocardium offer a potentially promising role in promoting recovery from injury. (stanford.edu)
  • In its chemically pure state, occurs in two phases: α- found naturally and β- a result of refining, is the predominant phase in dental materials 4 . (bvsalud.org)
  • This risk was also raised at some stage in Germany, because of the fear that this technique would generate bio-aerosol drops, which could promote virus spread. (springer.com)
  • Separation technique in which the stationary phase consists of ion exchange resins. (lookformedical.com)
  • The composition of the mobile phase along with the C18 column was the key to successful separation. (aacc.org)
  • In Biodine V different plants cooperate to mimic the composition and the liquid crystal structure of our skin. (cosmeticsbusiness.com)
  • Biodine V repairs this damage instantly thanks to its similar composition and liquid crystal structure. (cosmeticsbusiness.com)
  • As transcription silencing is key for establishing the HIV reservoir, significant efforts have been made to understand the mechanism that regulate HIV gene transcription, and the role of the elongation machinery in promoting this step. (biomedcentral.com)
  • Surgical operations to reestablish and promote further development of a pulmonary arterial connection preferentially utilize MAPCAs for reconstruction of central pulmonary arteries. (stanford.edu)
  • To investigate how this dichotomy in cellular behavior is controlled in vivo , we used C. elegans , leveraging its highly stereotypical development ( Sulston and Horvitz, 1977 ), as well as its genetic and optical tractability. (elifesciences.org)
  • The session will take place in Portuguese, with the exception of the last talk which will be in English: SAVE THE MICROBES TO SAVE THE PLANET to be presented by the President of the International Union of Microbiological Societies (IUMS), it is a call to action to promote the development of sustainable solutions to control infectious agents, preserve global microbial diversity and healthy life on our planet. (scoop.it)
  • We propose a model in which association of NHR-67 with the Groucho/TCF complex suppresses the default invasive state in non-invasive cells, which complements transcriptional regulation to add robustness to the proliferative-invasive cellular switch in vivo . (elifesciences.org)
  • interestingly, antibodies against granulocyte macrophage colony-stimulating factor, interferon, interleukin 1, and interleukin 6 in immune globulin have biologic activity in vivo. (medscape.com)
  • Recent studies have shown that phase separation is closely related to neurodegenerative diseases, cancer , virus infection and other major diseases are closely related, and disease treatment targeting the phase separation process has become a new frontier of biomedicine. (medicaltrend.org)
  • A properly shaped and disinfected root canal system is an environment in which the bacterial communities have been disrupted or eliminated, ensuring that the environment is, unable to promote or sustain periapical disease. (bvsalud.org)
  • Here, we investigated this dichotomy in vivo by examining two cell types in the developing Caenorhabditis elegans somatic gonad that derive from equipotent progenitors, but exhibit distinct cell behaviors: the post-mitotic, invasive anchor cell and the neighboring proliferative, non-invasive ventral uterine (VU) cells. (elifesciences.org)
  • A liquid, pasteurized, 10% concentrated intravenous gammaglobulin preparation is as effective as a 5% concentrated preparation. (medscape.com)
  • A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. (lookformedical.com)
  • Chromatographic techniques in which the mobile phase is a liquid. (lookformedical.com)
  • Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase). (lookformedical.com)
  • 2 lamellar phase towards the top and pure 2H O in the bottom. (lu.se)
  • Inhalation Toxicology Downloaded from informahealthcare.com by CDC Information Center on 07/06/12 stored as a liquid under pressure, but volatilizes rapidly fol- to agricultural soil is expected to occur over a time period lowing injection into soil. (cdc.gov)
  • insert":{"user_id":"R000005835","type":"published_papers"},"similar_merge":{"see_also":[{"@id":"https://www.ncbi.nlm.nih.gov/pubmed/35286679","label":"url"},{"@id":"https://web.db.tokushima-u.ac.jp/cgi-bin/edb_browse?EID=399625","label":"url"}],"paper_title":{"en":"Live Imaging of cAMP Signaling in D. discoideum Based on a Bioluminescent Indicator, Nano-lantern (cAMP). (tokushima-u.ac.jp)
  • Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. (lookformedical.com)
  • Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. (lookformedical.com)
  • Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. (lookformedical.com)