Vivo precursor cells b lymphoid. Medical search. Frequent questions

Further, T-BET- and EOMES-deleted CD56bright NK cells acquired an innate lymphoid cell precursor-like (ILCP-like) profile with increased expression of the ILC-3-associated TFs RORC and AHR, revealing a role for T-box TFs in maintaining mature NK cell phenotypes and an unexpected role of suppressing alternative ILC lineages. (bvsalud.org)
The developmental branching points between several lymphoid and myeloid lineages are still controversial, and little is known about how their diversification is induced. (scielo.br)
Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
A wide variety of hematopoietic cells exist, with the major protective lineages being lymphoid and myeloid. (justia.com)
The immune system which results from cells of the lymphoid and myeloid lineages is developed in vivo, so as to recognize self from non-self. (justia.com)
HSCs are able of distinguishing into the several myeloid and lymphoid lineages while MSCs maturate to the several mesodermal lineages including adipocytes, osteoblasts and fibroblasts. (cancer8.info)
GRPs are early precursors within the oligodendrocytic and astrocytic cell lineages and are described by their reflection of A2C5 [46, 47]. (cancer8.info)
There are many subsets of DCs with different phenotypes derived from either conventional or lymphoid lineages. (biomedcentral.com)
Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)

Medullary epithelial cells (mTEC), in conjunction with dendritic cells, then take over control of developing thymocytes. (frontiersin.org)
In this study, the finding that a significant proportion of all dendritic cells (DCs) resident in vivo in the human postnatal thymus displayed a myeloid-related phenotype prompted us to re-examine the developmental origin of thymic DCs, a cell type hitherto considered to represent a homogeneous lymphoid-derived population. (ashpublications.org)
Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)
Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)
CD4 has also been detected on pluirpotent hematopoietic stem cells, bone marrow myeloid precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. (bdbiosciences.com)
Dendritic cells-based therapeutic approach may be a potential strategy for SARS-CoV-2 infection. (medsci.org)
Basis of treatment based dendritic cells to combat coronavirus infections is summarized. (medsci.org)
Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. (biomedcentral.com)
Dendritic cells (DCs) are the most important antigen-presenting cells (APCs) in the body. (biomedcentral.com)

Using these results, we generated a chimeric avian-mammalian MXRA8 decoy-receptor that neutralizes infection of multiple alphaviruses from distinct antigenic groups in vitro and in vivo. (bvsalud.org)
BMSCs can also be engineered to secrete a variety of different proteins in vitro and in vivo that could potentially treat a variety of serum protein deficiencies and other genetic or acquired diseases [ 13 ]. (hindawi.com)
Here we review the in vitro and in vivo evidence of this intriguing phenomenon. (mssm.edu)

While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)
Although engraftment of irradiated C.B-17 scid/scid (SCID) mice with human progenitor cells occurred after infusion with human pediatric bone marrow cells, significant engraftment of the mouse bone marrow with human cells was dependent upon continuous treatment with exogenous human cytokines. (elsevierpure.com)
1) Estimation of the numbers of transduced progenitor cells by monitoring unique positions of integration of the therapeutic gene transfer vector. (europeanhealthjournal.com)
Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
we evaluate the different types of come cells and progenitor cells that are potential candidates for restorative strategies in perinatal mind accidental Rabbit Polyclonal to DRP1 injuries, and sum it up recent preclinical and medical studies. (cancer8.info)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

Her major scientific contributions are in the areas of T cell immune tolerance, T cell memory and development of intraepithelial lymphocytes. (wikipedia.org)
In post-natal life, SDF-1 is widely expressed and is induced in chronically inflamed tissues such as psoriatic skin and the rheumatoid synovium, but has also been implicated in the migration of lymphocytes to lymphoid organs. (biomedcentral.com)
In a very interesting chapter, Z. PANCER and M. D. COOPER first consider the emergence of lymphocytes as a novel circulatory cell type in vertebrates and then discuss phylogenetic aspects of the superfamily of LRR - containing proteins and their role in immunity. (scielo.br)
In the context of cancer, appropriately activated DCs can induce anti-tumor immunity by activating innate immune cells and tumor-specific lymphocytes that target cancer cells. (mdpi.com)
20. Melchers, F.: Biochemical characterization of immunoglobulin-producing B-lymphocytes at different stages of their differentiation.In: 'Regulation of Growth and Differentiated Function in Eukaryotic Cells,' eds. (uni-mainz.de)
Characterized by cell-surface molecules including CD103, CD69, and CD49a, T RM -like tumor-infiltrating lymphocytes (TILs) can be found in a wide range of human cancers, where they portend improved prognosis. (frontiersin.org)
The method entails optionally subjecting a sample to conditions suitable for expansion of one or more of the lymphocytes to form an expanded sample comprising antibody secreting cells (ASCs) and distributing the sample into containers to provide a plurality of subsamples. (justia.com)
Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

This model provides a powerful tool to investigate the pathways involved in cell-migration into lymphoid organs and potentially to target them for therapeutic purposes. (biomedcentral.com)
6 , 7 Murine DCs with the reciprocal CD8α + CD11b − phenotype, although present at various levels in all lymphoid organs, represent the prototype of the DC population resident in the postnatal thymus. (ashpublications.org)
In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs. (justia.com)
The field of the subject invention is the use of major histocompatibility complex antigen lacking cells and organs which may serve as universal donors in cellular and organ therapies including transplantation and to produce chimeric non-human mammals. (justia.com)
Classical definitions of memory T cells derive from the belief that T cells localize and recirculate predominantly throughout the blood and secondary lymphoid organs ( 7 , 8 ). (frontiersin.org)
Cells Tissues Organs , 171 (1), 38-43. (mssm.edu)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

We show here that these novel intrathymic DCs are truly myeloid, as they arise from CD34 + early thymic progenitors through CD34 lo intermediates which have lost the capacity to generate T cells, but display myelomonocytic differentiation potential. (ashpublications.org)
Signal transduction down the Ras/MAPK pathway, including that critical to T cell activation, proliferation, and differentiation, has been generally considered to occur at the plasma membrane. (scielo.br)
TGF-β affects development and differentiation of human natural killer cell subsets. (sunnybrook.ca)
HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation. (sunnybrook.ca)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
A long-term multi-organ co-culture system coupled with quantitative imaging to visualize blood cell cycle progression, analyze polarized blood cell mitosis, and track blood cell differentiation kinetics during Drosophila hematopoiesis in homeostatic condition and following infection. (elifesciences.org)
During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
the gene expression profile during B lymphoid differentiation. (lu.se)

Our results indicate a reduction in vascular placental cells, especially cells responsible for structural integrity, and increase in trophoblast proliferation in animals exposed to particulate matter. (nature.com)
Mechanistically, T-BET and EOMES were required for normal NK cell proliferation and persistence in vivo. (bvsalud.org)
MicroRNA miR-199a-3p regulates cell proliferation and survival by targeting caveolin-2. (sunnybrook.ca)
stream ATP-binding cassette transporters and HDL suppress hematopoietic stem cell proliferation. (skanestugan.se)
NMN-treated T cells showed a superior rate of proliferation and remained active and cytotoxic long after the controls died. (lifespan.io)
In: 'Control of Proliferation in Animal Cells', Cold Spring Harbor Laboratory, pp. 393-410, 1974. (uni-mainz.de)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)

Mutation of the IL2RG gene results in a form of severe combined immune deficiency (SCID-X1), which has been treated successfully with hematopoietic stem cell gene therapy. (europeanhealthjournal.com)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
In contrast to their wild type counterparts, Pax-5 -/- pre-B I cells posses an extraordinary developmental plasticity showing hematopoietic stem cell features such as pluripotency and self renewing capacity. (mssm.edu)
definitive treatment is hematopoietic stem cell transplantation. (msdmanuals.com)

Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
Immature B cells from normal mouse bone marrow were not responsive to the suppressive effect of androgens unless they were cocultured with marrow stromal cells or with supernatants from androgen-treated stromal cells, suggesting that the androgen effects are exerted through marrow stromal elements by production of a diffusible mediator. (jci.org)
Further experiments revealed that bone marrow stromal cells produced TGF-β in response to dihydrotestosterone (DHT), and neutralization of TGF-β in the DHT-treated stromal cells reversed the suppressive effects. (jci.org)
The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
These experiments suggest that effects of androgens on developing B cells are mediated through androgen receptors in bone marrow stromal cells. (jci.org)
Several lines of evidence support the hypothesis that gonadal steroids exert effects on the development of B cells in bone marrow. (jci.org)
Androgens also exert effects on B cell development in bone marrow. (jci.org)
In both patients, CTL019 T cells expanded to a level that was more than 1000 times as high as the initial engraftment level, and the cells were identified in bone marrow. (nih.gov)
SDF-1 (CXCL12), a CXC chemokine, has a primary role in signalling the recruitment of haematopoietic stem-cell precursors to the bone marrow during embryonic development. (biomedcentral.com)
In the present study, after infusion of irradiated SCID mice with pre-cultured human fetal bone marrow cells (BM-SCID-hu mice), their bone marrow became significantly engrafted with human precursor cells and their peripheral lymphoid compartment became populated with human B cells and monocytes independently of the administration of extraneous human cytokines. (elsevierpure.com)
Since engraftment occurs in the absence of exogenous cytokine treatment, the BM- SCID-hu mouse model described should provide a useful in vivo system for studying factors important in the maturation of human myeloid and lymphoid cells in the bone marrow and the behavior of the mature human cells after dissemination into the peripheral lymphoid tissue. (elsevierpure.com)
The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
In other cases, where certain cells are lacking, such as islets of Langerhans in the case of diabetes, or cells which secrete dopamine in the case of Parkinson's disease, or bone marrow cells in various hematopoietic diseases, or muscle cells in muscle wasting disease, or retinal epithelial cells in visual disorders, it would be desirable to be able to provide cells which could fulfill the desired function. (justia.com)

dATP and dGTP predominantly accumulates in lymphoid tissue. (medscape.com)
Interestingly, the transcriptional response of skin fibroblasts to IL-4 converged with that of TNF-alpha-treated synovial fibroblasts, suggesting resident tissue fibroblasts and their blood-borne precursors may be imprinted by inflammatory cytokines that are characteristic of different tissues. (birmingham.ac.uk)
In addition grafts retain some of the histological features of the pretransplantation tissue, such as follicular dendritic cell-associated B-cell aggregates, lymphatic and HEV markers. (biomedcentral.com)
However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
Moreover, it has been proven to play important roles under disease and stress situations by maintaining epithelial integrity and gut associated lymphoid tissue (GALT) function [ 3 , 6 , 7 ]. (biomedcentral.com)
Resident memory (T RM ) cells are a distinct tissue-localized T cell lineage that is crucial for protective immunity in peripheral tissues. (frontiersin.org)
Recent studies in mouse tumor models have shown that T RM cells are induced by cancer vaccines delivered in peripheral tissue sites, or by the depletion of regulatory T cells. (frontiersin.org)
Such tumor-specific T RM cells are recognized as both necessary and sufficient for long-lived protection against tumors in peripheral tissue locations. (frontiersin.org)
To this end, multiple tissue-resident cells and recruited immune cells cooperate to efficiently repair the injured tissue. (biomedcentral.com)
Loss of tissue is then repaired in a complex process that involves tissue-resident immune, stromal, and epithelial cells, as well as infiltrating immune cells [ 2 ]. (biomedcentral.com)
Specifically, the timely orchestration of pro- and anti-inflammatory responses, based on the activation and inhibition of various cell types, such as tissue-resident cells and different types of immune cells, can significantly impact wound repair [ 7 ]. (biomedcentral.com)
Here, we summarise the mechanisms contributing to adipose tissue (AT) plasticity and function including characteristics and cellular complexity of the various adipose depots and we discuss recent insights into AT origins, identification of adipose precursors, pathophysiological regulation of adipogenesis and its relation to WAT/BAT expandability in obesity and its associated comorbidities. (springer.com)
Autofluorescence imaging permits label-free cell type identification in live airway tissue and reveals the dynamic formation of airway secretory cell associated antigen passages. (elifesciences.org)

In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)
Moreover, although interleukin 7 (IL-7) supports the generation of such myeloid intermediates, we show that their developmental branching from the main intrathymic T-cell pathway is linked to the up-regulation of the myelomonocytic granulocyte macrophage-colony-stimulating factor (GM-CSF) receptor, to the down-regulation of the IL-7 receptor and to the lack of pre-T-cell receptor α (pTα) gene transcriptional activation. (ashpublications.org)
8 As these latter DCs derive from an intrathymic lymphoid-restricted progenitor able to generate also T, B, and NK cells, but not myeloid cells, upon transfer in vivo, 5 , 9 , 10 they have hitherto been considered of lymphoid origin. (ashpublications.org)

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) (also known as CD152) is a major negative regulator of T cell responses which interacts with the B7-1 (CD80) / B7-2 (CD86) ligands on the surface of an antigen-presenting cell. (scielo.br)
Jawed vertebrates (gnathostomes) generate a diverse repertoire of B and T cell antigen receptors through the rearrangement of immunoglobulin V, D, and J gene fragments, whereas jawless fish (agnathans) assemble diverse lymphocyte antigen receptor genes through the genomic rearrangement of leucine-rich repeat (LRR) - encoding molecules. (scielo.br)
Diagnosis is by detecting lymphopenia, absence or a very low number of T cells, and impaired lymphocyte proliferative responses to mitogens. (msdmanuals.com)

While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)

Taken together, these data challenge the current view that the thymus is colonized by a lymphoid-restricted progenitor and provide evidence that a more immature precursor population with lymphoid and myelomonocytic potential is actually seeding the human postnatal thymus. (ashpublications.org)
Although NK precursors and immature NK cells have been identified in mice and humans, the precise sites where NK cell maturation take place are not yet fully defined. (scielo.br)
However, clear evidence for functional NK cell subsets exists in humans and the presence of NK cells in the thymus and immature NK cells in the liver suggests specific functions for NK cells in the tissues. (scielo.br)

In a further description of the mechanism of T-cell depletion in purine nucleoside phosphorylase deficiency, Arpaia et al reported increased in vivo apoptosis of T cells and increased in vitro sensitivity to gamma irradiation in a murine model. (medscape.com)
The exhaustion of T cells that prevents them from attacking cancer cells manifests itself, in large part, in cellular senescence and increased apoptosis. (lifespan.io)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)

CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. (bdbiosciences.com)

The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)
Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)

Cortical epithelial cells (cTEC) support the earliest thymocyte progenitor populations, guiding them through the cortex, directing them to a T-cell lineage fate and ensuring their functional qualities as self-MHC restricted cells through the process of positive selection ( 2 ). (frontiersin.org)
whereas in PNP deficiency, the metabolites are especially toxic to T-lineage cells, resulting in profound T-cell deficiency and variable degree of B-cell dysfunction. (medscape.com)
In both metabolic disorders, the enzyme deficiencies cause the accumulation of metabolites that are toxic to lymphoid lineage cells. (medscape.com)
Next, an overview of several current models of hematopoietic lineage trees to identify developmental branching points between the lymphoid and myeloid cell compartments is discussed. (scielo.br)
Enforcement of γδ-lineage commitment by the pre-T-cell receptor in precursors with weak γδ-TCR signals. (sunnybrook.ca)
Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. (mdpi.com)
Lymphoid lineage DCs primarily differentiate into plasmacytoid DCs and occupy approximately 0.5% of peripheral blood mononuclear cells (PBMCs) in humans [ 2 ], but the cell population percentage is unclear in horses. (biomedcentral.com)

1-5 In mice, DCs bearing the myeloid marker CD11b (Mac-1), but lacking CD8α, derive efficiently from myeloid precursors and represent the major DC subset in the periphery. (ashpublications.org)
MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. (bdbiosciences.com)
These early classifications of T cell memory were quickly brought to bear on the question of what T cell subset provides the best immunity against cancer. (frontiersin.org)
Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)

Tissues, including the placenta, are comprised of diverse cell types with distinguishable developmental or functional origin that form a complex niche 1 . (nature.com)
Purine nueoside phosphorylase (PNP) deficiency causes a form of severe combined immunodeficiency (SCID) characterized by profound T cell deficiency, failure to thrive (FTT), recurrent deep seeded infection, developmental delay, progressive neurological deterioration, and autoimmune complications. (medscape.com)
1-3 These nonoverlapping functions have been proposed to result from the actions of 2 major DC populations which have been characterized as myeloid and lymphoid DCs, respectively, on the basis of their anatomical localization and cell-surface phenotypes and, ultimately, of their distinct developmental origin. (ashpublications.org)
Developmental progression of fetal HEB(-/-) precursors to the pre-T-cell stage is restored by HEBAlt. (sunnybrook.ca)

Androgens only affected B cell development in chimeric mice with androgen-sensitive stromal cells. (jci.org)
These microenvironments are classically defined as distinct cortex and medulla regions that each contain specialized subsets of stromal cells. (frontiersin.org)
Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)

Co-culture of skin or synovial fibroblasts with synovial fluid-derived mononuclear cells provided further evidence that these transcriptional differences were functionally significant in an ex vivo setting. (birmingham.ac.uk)
We also demonstrate that phenotypically and functionally equivalent myeloid precursors devoid of T-cell potential do exist in vivo in the postnatal thymus. (ashpublications.org)
Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature. (sunnybrook.ca)

Chimeric antigen receptor-modified T cells with specificity for CD19 have shown promise in the treatment of chronic lymphocytic leukemia (CLL). (nih.gov)
It remains to be established whether chimeric antigen receptor T cells have clinical activity in acute lymphoblastic leukemia (ALL). (nih.gov)
Two children with relapsed and refractory pre-B-cell ALL received infusions of T cells transduced with anti-CD19 antibody and a T-cell signaling molecule (CTL019 chimeric antigen receptor T cells), at a dose of 1.4×10(6) to 1.2×10(7) CTL019 cells per kilogram of body weight. (nih.gov)
In addition, the chimeric antigen receptor T cells were observed in the cerebrospinal fluid (CSF), where they persisted at high levels for at least 6 months. (nih.gov)
cytokine blockade with etanercept and tocilizumab was effective in reversing the syndrome and did not prevent expansion of chimeric antigen receptor T cells or reduce antileukemic efficacy. (nih.gov)
Chimeric antigen receptor-modified T cells are capable of killing even aggressive, treatment-refractory acute leukemia cells in vivo. (nih.gov)
2) Estimation of T cell population structure by sequencing of the recombined T cell receptor (TCR) beta locus. (europeanhealthjournal.com)
Versican G3 promotes mouse mammary tumor cell growth, migration, and metastasis by influencing EGF receptor signaling. (sunnybrook.ca)
The researchers experimented on the most popular CAR-T cells that express the receptor CD19 and are used against several types of blood cancer, such as B-cell acute lymphoblastic leukemia. (lifespan.io)
It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine Lck. (bdbiosciences.com)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
Recent evidence obtained in mouse models shows its essential role regulating blood cell function through various mechanisms that include pseudohypoxia responses by hypoxia-inducible factor-1α activation, post-translational modifications like succinylation, and communication mediated by succinate receptor 1. (haematologica.org)
GPR91 is a G protein-coupled cell surface receptor for extracellular succinate (Sucnr1). (haematologica.org)
The thymic selection of the human T-cell receptor repertoire releases polyspecific receptors with the ability to recognize and respond to peptides from unrelated viruses. (elifesciences.org)
In healthy individuals cells ingest cholesterol by endocytosis of LDL bound to the LDL-receptor (LDLR). (biomedcentral.com)

Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
TATA-box binding protein is not required for RNA Polymerase II transcription in mouse embryonic stem cells. (elifesciences.org)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)

In CAR-T cell-based immunotherapy, a bunch of T cells are procured from the patient's blood, genetically engineered to express the relevant CAR, and injected back into the patient's body. (lifespan.io)
As the field of cancer immunotherapy advances rapidly, it is now crucial to understand how the dissemination and maintenance of tumor-specific T cells can be optimally achieved. (frontiersin.org)

Stromal cell derived factor-1 (CXCL12) induces cell migration into lymph nodes transplanted into SCID MICE. (biomedcentral.com)
To investigate the role of SDF-1 in recirculation and homing in vivo we have developed a model in which human peripheral lymph nodes (huPLN) are transplanted into SCID mice. (biomedcentral.com)
This is the first description of huPLN transplantation into SCID mice, and of the functional effects of SDF-1 regarding the migration of human cells into huPLN in vivo . (biomedcentral.com)
Furthermore, despite cytokine treatment, only minimal peripheral engraftment of these mice with human cells was observed. (elsevierpure.com)
Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
In Pax-5 deficient mice, B cell development is blocked at the pre-B I cell stage. (mssm.edu)
Here we comprehensively characterized the kinetics of cell-mediated and humoral immune responses in VRP-vaccinated mice, and demonstrate that they predominantly target the nucleoprotein (NP). (cdc.gov)

The antibody response to HIV-1 in vivo is direct against several viral proteins. (scielo.br)
There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
This review summarizes some immunological factors involved in the development and control of this oral disease, such as: the participation of inflammatory cells in local inflammation, the synthesis of chemotaxis proteins with activation of the complement system and a range of antimicrobial peptides, such as defensins, cathelicidin and saposins. (bvsalud.org)

We employed in vitro cultures and two in vivo models to investigate whether androgens exert these effects directly on marrow lymphoid precursors or whether actions on marrow stromal elements are required. (jci.org)

Resident memory CD8 + T (Trm) cells stably reside in non-lymphoid tissues and mediate superior innate and adaptive immunity against pathogens. (lifeboat.com)
NK cells coordinate tumor immunosurveillance and the immune response against pathogens. (scielo.br)
This lets us get rid of cells that are infected with pathogens, such as viruses and bacteria, along with cells that have become cancerous. (lifespan.io)
The protective mechanism which protects the host from disease, as a result of invasion of viruses, bacteria, or other pathogens, is also able to recognize cells which come from a different mammalian host, even an allogeneic host. (justia.com)
It is likely that L-DCs play an important role in antigen uptake and processing of respiratory pathogens and are major contributors to respiratory tract immunity and may be ideal tools for future in vitro or ex vivo studies. (biomedcentral.com)

Single-cell RNA-Seq revealed a specific T-box transcriptional program in human NK cells, which was rapidly lost following T-BET and EOMES deletion. (bvsalud.org)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)

Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
This proposal is further supported by the finding that development of thymic DCs and T cells is linked via a common precursor at an early stage of thymocyte development. (ashpublications.org)

Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)
Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)

He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
This promising field may open new avenues to modulate inflammatory responses and to preserve blood cell homeostasis in the clinical setting. (haematologica.org)
CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
The integration of pathogen-associated molecular patterns (PAMPs) from microorganisms with their surface receptors in the immune cells, induces the production of several cytokines and chemokines that presents either a pro- and/or anti-inflammatory role by stimulating the secretion of a great variety of antibody subtypes and the activation of mechanisms of controlling the disease, such as the regulatory T cells. (bvsalud.org)

Placental transcriptomes, at day 19 of gestation, were determined using bulk RNA-seq from whole placentas of exposed (n = 4) and control (n = 4) animals and scRNAseq of three distinct placental layers, followed by flow cytometry analysis of the placental immune cell landscape. (nature.com)
Tumor development has been said to occur in three distinct steps: inefficient elimination of early transformed cells, development of a state of equilibrium between tumor cells and immune cells, and tumor escape from immune pressure ( 2 ). (frontiersin.org)
Integrating this data unveiled 107 distinct cell types and their specific utilization of 544,735 candidate cis-regulatory DNA elements (cCREs) in the human genome. (salk.edu)

Mol Cell Biol. (sunnybrook.ca)

Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors. (sunnybrook.ca)
Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)

In this review, we cover how the non-epithelial compartment of thymic stroma represent essential cell populations in regulating thymus function. (frontiersin.org)
SCID-X1 gene therapy results in reconstitution of the previously lacking T cell compartment, allowing analysis of the roles of T cell immunity in humans by comparing before and after gene correction. (europeanhealthjournal.com)

Transfusion of autologous tumor-specific CTLs 1-4 and blockade of T cell inhibitory receptors 5-7 have demonstrated to elicit durable clinical benefit in a significant proportion of patients with melanoma, leukemia, lymphoma and other cancers, who failed to respond to conventional treatments. (lifeboat.com)
After being treated with a dose of NMN, the cells were co-cultured with NALM6 cells, a model of leukemia. (lifespan.io)
Finally, the scientists tested NMN-CAR-T cells in a mouse model of B-cell lymphoid leukemia. (lifespan.io)
In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)
Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)

Memory CD8 + T cell responses have the potential to mediate long-lasting protection against cancers. (lifeboat.com)
Emerging evidence indicates that Trm cells develop in human solid cancers and play a key role in controlling tumor growth. (lifeboat.com)
Scientists have been trying to overcome this problem by genetically modifying T cells to express receptors specifically tuned to recognize various cancers. (lifespan.io)
The recent success of T cell immune checkpoint inhibitor (ICI) therapies for cancer has revealed CD8 T cells as potent mediators of immunity against advanced cancers ( 4 , 5 ). (frontiersin.org)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
Lymphoid neoplasms are cancers of the immune system, which afflict both adults and children, and account for 6-10% of all neoplastic diseases. (univ-mrs.fr)
Trained immunity of natural killer (NK) cells has shown great potential in the treatment of cancers by eliciting enhanced effector responses to restimulation by cytokines or cancer cells for long time periods after preactivation. (cancerbiomed.org)

However, the specific contribution of Trm cells to anti-tumor immunity is incompletely understood. (lifeboat.com)
8-10 Long-lasting protective immunity relies on the efficient establishment of long-lived memory CD8 + T cells, which have the potential to eradicate primary and disseminated tumors. (lifeboat.com)
Vaccine strategies that concentrate on stimulating T cell immunity will at best generate reactive and persisting T cell responses that can suppress virus without preventing infection, limiting or preventing the damage the virus cause. (scielo.br)
While a great deal of effort has focused on defining their role in immunity to infections, studies now reveal T RM cells as a vital component of the host immune response to cancer. (frontiersin.org)
This review will recount the path to discovering the importance of resident memory CD8 T cells as they pertain to cancer immunity. (frontiersin.org)
In addition to highlighting key studies that directly implicate T RM cells in anti-tumor immunity, we will highlight earlier work that implicitly suggested their importance. (frontiersin.org)
However, the human NK cells responsible for the generation and maintenance of trained immunity are largely unknown. (cancerbiomed.org)

The emergence of tumor cells that no longer express the target indicates a need to target other molecules in addition to CD19 in some patients with ALL. (nih.gov)
These changes can be inherited and are, therefore, found in every cell, but more often, they are somatically acquired and restricted to tumor cells. (medscape.com)

The production of a diverse αβ-T-cell pool is vital to establishing and maintaining a functional adaptive immune system. (frontiersin.org)
T cells are a central element of the adaptive immune system, and some of them can be cytotoxic: they have the ability to kill other cells. (lifespan.io)

Lymphoma cells are reported to over-express chemokine receptors that favors leukocyte infiltration, promotes tumorigenesis, angiogenesis, immune evasion, and metastasis. (aacrjournals.org)
These are chimeric antigen receptors (CARs), and CAR-T cell therapies are already being used against some types of cancer. (lifespan.io)

The thymus supports T-cell development via specialized microenvironments that ensure a diverse, functional and self-tolerant T-cell population. (frontiersin.org)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
In brief, my research team uses cutting edge imaging, clonal tracking (cellular barcoding), genomics technologies, and functional assays (in vivo and in vitro) to tackle long-standing questions about the biology of blood production. (edu.au)

Investigation of human hematopoietic maturation has been hampered by the lack of in vivo models. (elsevierpure.com)

Galanin (GAL) is a neuropeptide of 29 or 30 (in humans) amino acids that is proteolytically processed from the peptide precursor preprogalanin. (hindawi.com)
Indeed a wealth of studies from humans and mouse models establishes a particularly potent role for CD8 T cells in controlling the outgrowth of malignancies ( 3 ). (frontiersin.org)

Intradermal, but not intraperitoneal vaccination, generated memory precursors expressing skin-homing molecules in circulation and Trm cells in skin. (lifeboat.com)
The use of cell lines as "biological minipumps" to chronically deliver antinociceptive molecules into the pain-processing centers of the spinal cord represents a newly developed technique for the treatment of pain [ 3 ]. (hindawi.com)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)

Here we interrogate T cell reconstitution using four forms of high throughput analysis. (europeanhealthjournal.com)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)

11 They have been typically classified in two subsets: effector-memory (Tem) and central-memory (Tcm) CD8 + T cells. (lifeboat.com)
Such memory T cells were traditionally defined as being comprised of both central memory (T CM ) and effector memory (T EM ) subsets ( 9 ). (frontiersin.org)
In interaction with other non-immune cells, such immune cell subsets ensure the functionality and integrity of the skin layers [ 1 ]. (biomedcentral.com)

When the host lacks the ability to raise a defense against a particular disease, there may also be an interest in administering specific T-cells or B-cells or precursors thereof which may supplement the host's compromised immune system. (justia.com)
In order for the cells to be effective, they must be safe from attack by the host, so that they may function without being destroyed by the immune system. (justia.com)
It is therefore of interest to find effective ways to produce cells which may function, proliferate, and differentiate as appropriate, while being safe from attack by a recipient's immune system. (justia.com)
In: 'Regulation of the Immune System: Genes and the Cells in which They Function,' Vol. VI, eds. (uni-mainz.de)
Dendritic cell as sentinel of the immune system plays an irreplaceable role. (medsci.org)
In the case of allogeneic transplantation, one of the major complications is definitely the immune system mediated response to the foreign cells. (cancer8.info)

During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)

The most generally utilized and most likely greatest defined of the SSCs are hematopoietic (HSC) and mesenchymal (MSC) control cells. (cancer8.info)

In mammals, the immune systems serves as the primary defense with many different types of cells and mechanisms to protect the host. (justia.com)
Although OECs do always suit the traditional explanation of control cells, their features make them feasible candidates for cell therapy as well as study into the mechanisms controlling myelination. (cancer8.info)
However, this approach requires prior in-depth understanding of the mechanisms by which lymphoid cells undergo neoplastic initiation and progression. (univ-mrs.fr)

The nuclear membrane protein SUN1 stabilizes endothelial cell-cell junctions far from the nucleus via regulation of microtubule dynamics and Rho GEF-H1 signaling, revealing long-range cellular communication important for vascular development and function. (elifesciences.org)

While innate immune cells are important for early tumor immune surveillance, T cells are fundamentally recognized for their crucial role in the antigen-specific recognition and elimination of malignantly transformed cells ( 2 ). (frontiersin.org)
Nonetheless, when cells rely on anaerobic glycolysis, like cancer cells and certain innate immune cells upon activation, other metabolic pathways sustain succinate levels, including glutamine-dependent anerplerosis to α-ketoglutarate, and eventually citrate by reductive carboxylation. (haematologica.org)

In a growing number of cases, CD8 T cells have been shown to mediate the regression of large bulky tumors, resulting in durable long-term disease remissions ( 5 ). (frontiersin.org)

Here, we demonstrated that intradermal administration of clinically relevant vaccines efficiently induces Trm cells specific for tumor-specific and self-antigens that accumulate in vaccinated and non-vaccinated skin. (lifeboat.com)
Here we demonstrated that a single intradermal administration of gene- or protein-based vaccines efficiently induces specific Trm cell responses against models of tumor-specific and self-antigens, which accumulated in vaccinated and distant non-vaccinated skin. (lifeboat.com)
The lymphoid system is predicated upon recognition of the presence of such MHC antigens as self. (justia.com)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

The placenta is a heterogeneous organ whose development involves complex interactions of trophoblasts with decidual, vascular, and immune cells at the fetal-maternal interface. (nature.com)
HEBAlt enhances the T-cell potential of fetal myeloid-biased precursors. (sunnybrook.ca)

The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)

The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)
Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
He is a member of the Stem Cell Network Centre of Excellence (past member of the Stem Cell Policy Committee and Research Management Committee and current Sub-chair of the Training and Education Committee), and a member of the AllerGen Network Centre of Excellence (Research Management Committee and Co-Chair of the Biomarkers Program). (ubc.ca)
Here, we further discuss current suggestions of the possible contribution of succinate to blood stem cell function and blood formation. (haematologica.org)
Stem Cell Res. (uh.edu)
Wu J, Hunt SD, Matthias N, Servián-Morilla E, Lo J, Jafar-Nejad H, Paradas C, Darabi R . Generation of an induced pluripotent stem cell line (CSCRMi001-A) from a patient with a new type of limb-girdle muscular dystrophy (LGMD) due to a missense mutation in POGLUT1 (Rumi). (uh.edu)

The cytokine-release syndrome and B-cell aplasia developed in both patients. (nih.gov)
NK cells lacking T-BET and EOMES also exhibited defective responses to cytokine stimulation. (bvsalud.org)

SCID-X1 gene therapy thus provides a unique opportunity to study the consequences of T cell function in previously deficient human subjects. (europeanhealthjournal.com)
The treatment also changed the gene expression profile of the cells. (lifespan.io)
In addition, heterozygous hosts having one wild-type gene and one mutated gene could be mated to obtain homozygous hosts, so that all of the cells would have the appropriate modification. (justia.com)
Delineating the gene-regulatory programs underlying complex cell types is fundamental for understanding brain function in health and disease. (salk.edu)
Integrative analyses revealed concordant changes in DNA methylation, chromatin accessibility, chromatin organization, and gene expression across cell types, cortical areas, and basal ganglia structures. (salk.edu)
This multimodal epigenomic brain cell atlas provides new insights into the complexity of cell-type-specific gene regulation in adult human brains. (salk.edu)
Our study shows how controlling for gene co-expression enables robust enhancer-gene linkage using single-cell sequencing data. (salk.edu)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)

Anatomy67

Organisms24

Diseases5

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes53

Disciplines and Occupations1

Information Science3

Lineages9

Dendritic cells9

Vitro and in vivo3

Progenitor cells6

Lymphocytes9

Organs7

Differentiation15

Proliferation8

Hematopoietic stem5

Bone14

Tissue13

Intrathymic3

Lymphocyte3

Peripheral lymphoid1

Immature3

Apoptosis4

Antigen-prese1

Pluripotent stem2

Lineage7

Subset4

Developmental4

Stromal cells3

Functionally3

Receptor15

Embryonic4

Immunotherapy2

Mice7

Proteins7

Employed in vitro1

Pathogens5

Transcriptional2

Thymocyte2

Epithelial cells2

Inflammatory4

Distinct3

Biol1

Progenitors2

Compartment2

Leukemia5

Cancers7

Immunity7

Tumor cells2

Adaptive immune2

Receptors2

Functional3

Maturation1

Humans2

Molecules3

Reconstitution2

Subsets3

Immune system6

Cytokines4

Mesenchymal1

Mechanisms3

Endothelial1

Innate immune cells2

Mediate1

Antigens4

Fetal2

Progression1

Stem cell6

Cytokine2

Gene9