Vivo pathway differentiation of cells. Medical search. Frequent questions

Therefore, mutations have been introduced by homologous recombination (HR) in F9 embryonal carcinoma (EC) cells, a cell-autonomous system that differentiates in the presence of RA, in order to disrupt RAR and RXR genes and establish their cellular and molecular functions in RA-induced differentiation. (degruyter.com)
We conclude that spatio-temporally controlled somatic mutations generated in animal models in given cell-types/tissues and at chosen times during pre- and postnatal life, are required to reveal the physiological and pathophysiological functions of the receptor genes involved in the retinoid signaling pathway throughout the life of the mouse. (degruyter.com)
In this study, we assayed the effect of exogenous mouse recombinant GDF-5 on cell proliferation of dental pulp cells and determined the expression levels of odontoblast differentiation marker genes in the cells of isolated mouse dental pulp tissues in the presence of GDF-5. (ispub.com)
Here, we investigated the effect of GDF-5 on the proliferation of dental pulp cells and their differentiation into odontoblasts by assaying the expression levels of odontoblast marker genes. (ispub.com)
Wortmannin, an inhibitor of PI3K, was used to further investigate the role of transcription factors FoxO1 and PPARγ and their downstream target genes in promoting adipocyte differentiation and lipid accumulation, and in regulating glucose and lipid metabolism. (biomedcentral.com)
We then proceed to characterize the identified genes in vivo and in vitro . (yale.edu)
By recruiting more than thousand kindreds with early onset CAD and multiple metabolic risk factors for genetics and metabolic studies we have been able to map, identify and characterize a dozen of human disease genes for CAD and MetS, which have been reported in leading journals such as Nat Genet, Science, NEJM, Cell Metab, JCI, PNAS, AJHG, etc. (yale.edu)
Subsequent molecular and physiological studies in human mutation carriers and animal models have allowed us to unravel novel functions of the identified genes, to delineate their cognate pathways and to discover new targets for pharmaceutical intervention. (yale.edu)
This task can be accomplished by replacing tumor suppressor genes that have been lost or mutated, selectively inserting genes that produce cytotoxic substances, or modifying the immune system to destroy the tumor cells. (medscape.com)
Between 15 and 18% of genes with DNA methylation changes were differentially expressed in CD4 + T cells in vivo. (biomedcentral.com)
Consistent with TP53-ARID1A mutual exclusivity, the p53 pathway is activated following ARID1A loss in the endometrial epithelium, where ARID1A normally directly represses p53 pathway genes in vivo, including the stress-inducible transcription factor, ATF3. (nih.gov)
At the molecular level, MWCNT exposure significantly increased the expression of the cell proliferation markers Ki-67 and PCNA and a panel of cell cycle-controlling genes in the lungs in a TIMP1- dependent manner. (cdc.gov)
Through the use of human embryonic stem cells (hESCs), we can mimic brain development towards different regions of the human brain, and thereby investigate the effect of novel genes on neural differentiation. (lu.se)
This team showed that injection of just three genes- Neurog3, Mafa, and Pdx1 -into the pancreatic parenchyma of mice leads to conversion of exocrine cells to functional beta cells, a process popularly known as "reprogramming. (medscape.com)
For example, how can genes be delivered at the precise dose to cells in humans? (medscape.com)

Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
To date, various studies have reported that BMP-family growth factors can induce cells isolated from dental pulp tissues to differentiate into odontoblasts. (ispub.com)
Yet it remains poorly understood how these factors can induce the various types of cells in the dental pulp to engage in a harmonized and coordinated process of dentin regeneration. (ispub.com)
Expression of exogenous wt-p53 in different tumor cell lines can induce growth arrest, apoptosis, or differentiation. (mcmaster.ca)
We speculate that Ad36 may induce adipocyte differentiation and changes of glucose and lipid metabolism by regulating PI3K/Akt/FoxO1/PPARγ signaling pathway. (biomedcentral.com)
Still missing from these approaches is any attempt to induce regeneration or replication of endogenous beta cells in the pancreas, an approach that would be physiologically desirable. (medscape.com)
The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)

Conclusion: A defined set of core signaling pathway mutations induces de-differentiation of cortical murine astrocytes into glioma stem cells. (nih.gov)
TCR stimulation via antigen/MHC presentation or anti-CD3 mAb induces T-ALL cell death. (aacrjournals.org)
We have also demonstrated that SPL metabolism is altered in "injured" corneal stromal cells, and that stimulation of healthy corneal stromal cells with Sphingosine 1-Phosphate (S1P), a bioactive SPL, induces TGF-ß1 expression and fibrosis, signaling through the S1P receptor 3 (S1P3). (hhs.gov)
Based on these discoveries, we hypothesize that S1P is a key mediator of corneal fibrosis via activation of TGF-ß, and TGF-ß in turn induces expression of S1P signaling proteins and thus forms a positive feedback loop which drives irreversible activation of corneal fibroblasts and differentiation to myofibroblasts. (hhs.gov)
By combinatorial screening using H4 and molecules with defined targets, we demonstrated that H4 induces differentiation by the activation of protein kinase C (PKC) signaling pathway, and in line with this, activates PKC phosphorylation and translocation of PKC to the cell membrane. (lu.se)
Right now, the most common theory is that the disease could potentially be triggered by an initial injury to the lung epithelium which in turn induces an abnormal repair pathway, leading to d amage of the lung. (lu.se)

Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)
There were no significant differences in dental pulp cell proliferation patterns related to the absence or presence of GDF-5 after seven days of culture. (ispub.com)
These results indicate that, although GDF-5 may have no effect on cell proliferation in isolated dental pulp tissue, it could promote odontoblast differentiation. (ispub.com)
2003). A previous in-vitro study on the role of GDF-5 in periodontal ligament cells has shown that exogenous GDF-5 promotes cell proliferation while suppressing the activity of alkaline phosphatase (ALP), which is closely related to tissue calcification and which is secreted during the formation of tissue calcifications. (ispub.com)
mTOR is normally a serine/threonine kinase having the ability to integrate environmental stimuli to modify cell metabolism success development and proliferation. (bioskinrevive.com)
RESULTS: The results of in vitro experiments showed that CHAG suppressed both basic and EGF-induced migration and invasion of OC cells, blocked the activation of EGF-initiated EGFR activation, inhibited downstream signal transduction of EGFR, and decreased expression of proliferation and migration/invasion related proteins. (bvsalud.org)
CONCLUSIONS: CHAG inhibited migration, invasion and proliferation of OC cells in vitro, and suppressed development of implantation tumor of OC in vivo. (bvsalud.org)
IL-15 is considered superior to IL-2 for cancer immunotherapy due to its strong bioactivity in improving CD8 + T and natural killer (NK) cell survival, proliferation and cytotoxic function ( 8 ). (frontiersin.org)
Androgens are also key regulators of immune cell function and have a suppressive influence on differentiation, proliferation, and cytokine production. (bham.ac.uk)
MWCNT-stimulated cell proliferation was most prominent in fibroblasts but not myofibroblasts. (cdc.gov)
Our study uncovers a new pathway by which induced TIMP1 critically modulates the pulmonary fibrotic response to MWCNTs by promoting fibroblast activation and proliferation via the TIMP1/CD63/integrin B1 axis and ERK signaling. (cdc.gov)
Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)

Insulin receptor substrate 1 plays a key role in transmitting signals from the insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF-1) to intracellular pathways PI3K / Akt and Erk MAP kinase pathways. (wikipedia.org)
This enables IRS-1 to activate several signalling pathways, including the PI3K pathway and the MAP kinase pathway. (wikipedia.org)
Ad36 may promote fatty acid and triglyceride synthesis, and improve insulin sensitivity by affecting the PI3K/Akt/FoxO1/PPARγ signaling pathway. (biomedcentral.com)
Molecular studies in mouse embryo fibroblast and human colorectal cancer cell lines defined APC/β-catenin and SRC-PI3K as key pathways in miR-135b activation. (gla.ac.uk)
The results of in vitro experiments with gastric cancer cell line AGS and hepatic cancer cell line HepG2 showed that CHAG inhibited the migration and invasion activities, the MAPK and PI3K/Akt mediated signaling, the activation of small G proteins Rac1 and RhoA, and the expression of MMPs and PCNA initiated by EGF, through blocking the activation of EGFR. (bvsalud.org)
In paper I, we identified and tested novel inhibitors targeting the PIM, PI3K, and mTOR pathways. (lu.se)

We developed techniques to isolate mouse OMSCs derived from mandibles and verified their MSC characteristics by single colony formation, multi-lineage differentiation, and in vivo tissue regeneration. (ca.gov)
TE strategies typically incorporate cells, biomaterials and signals (e.g. growth factors), with the goal of developing a construct that once implanted will promote tissue regeneration. (nature.com)
One of the potential limitations associated with the use of alginate for in vivo tissue regeneration is that it is in general non-degradable by mammals, as they lack the enzymes needed to break down the polymer chains 21 . (nature.com)
Inactivity impairs the balance of muscle protein synthesis and degradation and influences muscle-cell regeneration. (diabetesjournals.org)
His research interest lies in the development of in vitro and in vivo platforms for studying heart regeneration and precision medicine. (stanford.edu)
The successful generation of beta cells in vitro and regeneration of pancreatic beta cells in vivo will require a thorough understanding of the pathways that direct the normal development and function of these cells. (lu.se)
There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues. (nature.com)
Recently, a growing number of studies are focusing on mesenchymal stem cell-based therapies for neurodegenerative disorders. (hindawi.com)
The objective of this study was to provide a generalized critique for the role of mesenchymal stem cell therapy in ischemic stroke injury, its underlying mechanisms, and constraints on its preclinical and clinical applications. (hindawi.com)
The recent decade has seen encouraging outcomes of mesenchymal stem cell therapy that holds promise to alleviate the burden of neurological disorders Moreover, initial study data of preclinical trials have also indicated the effectiveness, tolerance, and safety of MSC-based therapy [ 10 ]. (hindawi.com)
The Jonason laboratory is interested in the signaling mechanisms that govern mesenchymal stem cell differentiation as well as maturation of committed chondrocytes and osteoblasts during skeletal development. (rochester.edu)

Evaluation of purified iNKT cells uncovered that TSC1 promotes T-bet which regulates iNKT maturation but downregulates ICOS appearance in iNKT cells by inhibiting mTOR complicated 1 (mTORC1). (bioskinrevive.com)
Furthermore mice missing T-bet exhibited both a terminal maturation defect of iNKT cells and a predominance of iNKT-17 cells and elevated ICOS appearance was necessary for the predominance of iNKT-17 cells in the populace of TSC1-lacking iNKT cells. (bioskinrevive.com)
Our data reveal that TSC1-reliant control of mTORC1 is essential Thy1 for terminal iNKT maturation and effector lineage decisions leading to the predominance of iNKT-1 cells. (bioskinrevive.com)
iNKT cell terminal maturation from levels 2-3 3 requires sign through the IL-15 and supplement D receptors as well as the transcription factor paederosidic acid T-bet and mediator subunit Med1 (11-14). (bioskinrevive.com)
To understand the molecular and cellular mechanisms that control beta cell maturation and function in the embryonic and adult pancreas. (lu.se)
This includes long-term survival of transplanted cells in vivo to assess cell fate, maturation and integration, as well as functional behavioural assessment for graft function in vivo. (lu.se)

Several years later, Kerr and co-workers 2 defined the morphological changes in cells undergoing this process, also known as apoptosis. (nature.com)
We discuss here the contribution of glycan-lectin interactions to the initiation, execution and resolution of apoptosis and their emerging roles in other cell death programs including autophagy. (nature.com)
In contrast, thymocytes that recognize self-peptide/MHC with high affinity undergo negative selection via TCR-mediated apoptosis, prompting Trinquand, dos Santos, Tran Quang, and colleagues to hypothesize that reactivation of this developmental checkpoint in T-ALL cells may have antileukemic activity. (aacrjournals.org)
miR-135b up-regulation resulted in reduced apoptosis and increased cell growth due to the downregulation of TGFRB2, DAPK1, APC, and FIH. (gla.ac.uk)
However, co-existing TP53-ARID1A mutations led to invasive adenocarcinoma associated with mutant ARID1A-driven ATF3 induction, reduced apoptosis, TP63+ squamous differentiation and invasion. (nih.gov)
In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)

At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
10 Galectins can function inside the cells by modulating signaling pathways, 11 although they also act extracellularly by establishing multivalent interactions with cell surface glycans and delivering signals that lead to disruption of cellular homeostasis. (nature.com)
Can we find common cellular pathways associated with photoreceptor cell death caused by distinct genetic mutations? (nih.gov)
The pathways involved in cellular differentiation or transformation are Smad, Rho proteins, and PI3-kinase. (molvis.org)
July 20, 2021 -- Cellular reprogramming of ex vivo cells may offer improvements on first-generation adoptive cell therapies, which have experienced limited clinical success due to complex manufacturing, potential patient rejection, and availability of clinical cell types. (scienceboard.net)
Techniques such as cellular reprogramming will allow the exploration of scalable allogeneic cell sources and the optimizable generation of cells for therapeutic development. (scienceboard.net)
Cellular reprogramming involves remodeling gene expression to generate a desired cell lineage or type. (scienceboard.net)
Advances in single-cell technologies and exponential growth of high-resolution biological data, harnessed by high-throughput bioinformatics and computational methodologies, have enabled the introduction of systematic means for controlling cellular identity. (scienceboard.net)
Together, our work demonstrates ILK as necessary for normal oligodendrocyte development, reinforces its role as a bridge between the actin cytoskeleton and cell membrane, and highlights the overarching compensatory capacity of oligodendrocytes in response to cellular milieu. (jneurosci.org)
Because secretion systems for Stx are lacking, the release of Stx is mediated through bacterial cell lysis by Stx phages in response to the induction of the SOS response (a cellular response to DNA damage) ( 11 ). (cdc.gov)
More striking, the pancreas in these mice appeared relatively normal, with well-demarcated islets, suggesting that Pax4 misexpression was inducing an orderly and spatially correct program of cellular conversion that probably recapitulated the normal cellular differentiation process. (medscape.com)

Our investigations utilize in vivo mouse retina and human retinal organoids derived from pluripotent stem cells as study systems. (nih.gov)
One is a method for in vitro differentiation of thyrocytes and 3D culture of follicles starting from mouse embryonic stem cells. (unil.ch)
We isolated a new population of stem cells from human orofacial tissue gingiva, a tissue source easily accessible from the oral cavity, namely GMSC, which exhibited clonogenicity, self-renewal, and multipotent differentiation capacities. (ca.gov)
Human orofacial bone-derived mesenchymal stem cells (OMSCs) showed distinct differentiation traits from mesenchymal stem cells (MSCs) derived from long bones, mouse OMSCs have not been isolated due to technical difficulties, which in turn precludes using mouse models to study orofacial diseases. (ca.gov)
Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
Zhou JQ, Wan HY, Wang ZX, Jiang N. Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells. (wjgnet.com)
Mesenchymal stem cells (MSCs), distributed in many tissues in the human body, are multipotent cells capable of differentiating in specific directions. (wjgnet.com)
The cells in the dental pulp comprise a heterogeneous mixed population, made up of odontoblasts, fibroblasts, stem cells, and macrophages and other immunocompetent cells. (ispub.com)
Introduction: Glioma stem cells isolated from human glioblastomas are resistant to radiation and cytotoxic chemotherapy and may drive tumor recurrence. (nih.gov)
Treatment efficacy may depend on the presence of glioma stem cells, expression of DNA repair enzymes such as methylguanine methyltransferase (MGMT), or transcriptome subtype. (nih.gov)
Serial neurosphere culture, multi-lineage differentiation, and orthotopic transplantation were used to assess whether these mutations induced de-differentiation of cortical astrocytes into glioma stem cells. (nih.gov)
Pediatric cancer treatments such as chemotherapy have recognized toxicity on germline stem cells, which could lead to infertility at adulthood ( 1 , 2 ). (elifesciences.org)
Our findings may have implications for research on liver differentiation from embryonic stem cells. (xenbase.org)
Mesenchymal stem cells up-regulate the invasive potential of prostate cancer cells via the eotaxin-3/CCR3 axis. (cancerindex.org)
This study aimed to clarify the role of mesenchymal stem cells (MSCs) as a component of the cancer microenvironment. (cancerindex.org)
Thus, we attempted to present an overview of previously published reports to evaluate the progress and provide molecular basis of mesenchymal stem cells (MSCs) therapy and its application in preclinical and clinical settings, which could aid in designing an effective regenerative therapeutic strategy in the future. (hindawi.com)
This concept gained prominence following Shinya Yamanaka's discovery of induced pluripotent stem cells (iPSCs) in 2007. (scienceboard.net)
Unfortunately, donor material is scarce and large efforts are made to develop beta cells from stem cells. (lu.se)
However, with a growing number of novel cell sources (stem cells and reprogrammed cells) it becomes important to develop new methodologies for a more thorough assessment of human neurons generated using these approaches. (lu.se)
The cells are called induced neurons (iNs) and offer a shortcut over induced pluripotent stem cells (iPSc) for generating patient and disease-specific neurons. (lu.se)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
A more appealing strategy involves the transplantation of beta cells grown from stem cells, particularly with recent advances in generating large numbers of beta cells from human stem cells. (medscape.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
All tissues originate from stem cells. (bvsalud.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

This identified IOX1, a pan-histone demethylase inhibitor, which selectively downregulates α-globin expression without perturbing erythroid differentiation or general gene expression, more specifically β-like globin expression. (haematologica.org)
In a matrigel invasion assay, interferon-gamma, a specific inhibitor of eotaxin-related homing, significantly reduced the transmigration of PC-3 cells, under co-cultured condition with MSCs, in a dose-dependent manner (P (cancerindex.org)
In vitro , MET overexpression activated ERK signaling and rendered BRAF-mutant colorectal cancer cells resistant to vemurafenib and panitumumab, and sensitivity was restored by treatment with the MET inhibitor crizotinib. (aacrjournals.org)
Functionally, BMP-9-treated HSVSMCs displayed a time-dependent increase in s-mothers against decapentaplegic (SMAD)1 phosphorylation paralleled by an increase in ID1 (encodes inhibitor of differentiation-1) mRNA expression levels indicating that BMP-9 activates the SMAD1 pathway in this cell type. (gla.ac.uk)
Furthermore, the combinatorial screening identified a bromo- and extra-terminal domain (BET) inhibitor that could further improve H4-dependent leukemic differentiation in FLT3 wild-type monocytic AML. (lu.se)

Results: G1/S-defective, Rb knockout astrocytes gained unlimited self-renewal and multi-lineage differentiation capacity, in both the presence and absence of Kras and Pten mutations. (nih.gov)

IRS-1 plays important biological function for both metabolic and mitogenic (growth promoting) pathways: mice deficient of IRS1 have only a mild diabetic phenotype, but a pronounced growth impairment, i.e. (wikipedia.org)
IRS-1 integrates signalling from insulin receptor (InsR), insulin-like growth factor-1 receptor (IGF1R) and many other cytokine receptors and is elevated in β-catenin induced cells. (wikipedia.org)
Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)
It is a highly biocompatible hydrogel whose physical properties can potentially be tailored to direct 3D cell growth and differentiation both in vitro and in vivo 10 , 11 , 12 . (nature.com)
These cells support tumour growth and recurrence and are resistant to radiotherapy and chemotherapy. (nih.gov)
Remarkably, pharmacological blockade of mGlu3 receptors stimulated the differentiation of cultured GICs into astrocytes, an effect that appeared to be long lasting, independent of the growth conditions, and irreversible. (nih.gov)
In in vivo experiments, a 3-month treatment with the brain-permeant mGlu receptor antagonist, LY341495 limited the growth of infiltrating brain tumours originating from GICs implanted into the brain parenchyma of nude mice. (nih.gov)
Growth/differentiation factor-5 (GDF-5) belongs to the bone morphogenetic protein (BMP) family, which is expressed in dental pulp tissues. (ispub.com)
To date, various studies have reported that cells isolated from the dental pulp of various animals can be induced to differentiate into cells of the odontoblastic phenotype by means of various chemicals, growth factors and signaling molecules both in vitro and in vivo ( reviewed in Nakashima and Akamine, 2005). (ispub.com)
Growth/differentiation factor-5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family, which is a subgroup of the transforming growth factor- (TGF-) superfamily. (ispub.com)
They leverage transcriptomic and epigenetic data to systematically identify key regulatory switches, such as the optimal combination of transcription factors or growth factors, required to directly differentiate any source cell type to any target cell type , as well as maintain cell identity . (scienceboard.net)
1 Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. (jci.org)
The corneal wound-healing, as well as the process of fibrosis, is driven by multiple complex pathways involving many cytokines, growth factors, and chemokines, which are not completely understood. (hhs.gov)
Our recent advances, with the aid of an NIH/NEI R21 (EY025256), have led us to discover a novel potential mechanism that combines sphingolipid (SPL) signaling with classical transforming growth factor-ß (TGF-ß) pathways to mediate corneal fibrosis via activation/differentiation of keratocytes into myofibroblasts. (hhs.gov)
Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)
The HER (erbB) family of transmembrane receptor tyrosine kinases is one of the cytostatic targets in tumor cell growth and survival. (medscape.com)
This family, which includes epidermal growth factor receptor (EGFR), plays a pivotal role in normal cell growth, lineage determination, repair, and functional differentiation. (medscape.com)
However, whether it can affect the implantation and growth of OC cells or not is still not clear. (bvsalud.org)
Meanwhile, results of in vivo experiments showed that CHAG not only inhibited the formation of implantation tumor of OC cells but also delayed the of the growth of the tumors. (bvsalud.org)
This paper was designed to investigate the effect of CHAG on metastasis and growth of tumor cells. (bvsalud.org)
In vivo experimental results showed that CHAG suppressed colonization, growth and metastasis of gastric cancer cell line SGC-7901 in peritoneal cavity of nude mice. (bvsalud.org)
In vivo, combination treatment resulted in slower tumor growth and increased survival of neuroblastoma PDX models. (lu.se)
In vivo, rigosertib treatment resulted in decreased tumor growth and increased survival of PDX mice. (lu.se)
Intraperitoneal administration of IL-15cx results in a moderate inhibition of breast cancer growth that is associated with an increase in the frequency of cytotoxic CD8 T cells and the improvement of their function. (frontiersin.org)
The depletion of myeloid-derived suppressor cells (MDSCs) has no impact on mouse breast cancer growth. (frontiersin.org)
Although a few FDA-approved therapies for type 2 diabetes (meglitinides, sulfonylureas, incretin-based drugs) directly enhance beta-cell function, no therapies for any form of diabetes lead to the growth of healthy, new beta cells to replace those that were lost or dysfunctional. (medscape.com)

Transcript levels of the androgen receptor (AR)-regulated gene Rhox5 were decreased at the end of the culture period, suggesting that testosterone production by Leydig cells and/or AR transcriptional activity was impaired in organotypic cultures ( 8 ). (elifesciences.org)
This result is particularly significant since 32D cells do not spontaneously express the CSF-1 receptor, whereas they undergo granulocytic differentiation upon G-CSF stimulation. (mcmaster.ca)
This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. (cancerindex.org)
During normal thymic development, T cells mature via cell surface expression of the T-cell receptor (TCR) and positive selection of thymocytes that bind self-peptide/MHC complexes with low affinity. (aacrjournals.org)
The iNKT-17 fate is normally developmentally programmed reliant on RORγt and favorably governed by IL-17 receptor B (17 19 On the other hand T-bet which is crucial for Th1 differentiation is vital for iNKT-1 (20 21 Nevertheless the romantic relationship between both of these iNKT effector lineages as well as the systems dictating iNKT-1 predominance over iNKT-17 are badly understood. (bioskinrevive.com)
Inhibition of the Prostaglandin EP-1 Receptor in Periosteum Progenitor Cells Enhances Osteoblast Differentiation and Fracture Repair. (rochester.edu)

How do inherited mutations affect photoreceptor homeostasis and cause cell death? (nih.gov)
HR in embryonic stem (ES) cells, has therefore been used to generate null mutations of the various RARs and RXRs in the mouse germline. (degruyter.com)
As reviewed here, the generation of such RAR and RXR germline mutations, combined with pharmacological approaches to block the RA signaling pathway, has provided many valuable insights on the developmental functions of RA receptors. (degruyter.com)
Methods: To model genetic alterations in the core signaling pathways of human glioblastoma, we induced conditional Rb knockout, Kras activation, and Pten deletion mutations in cortical murine astrocytes. (nih.gov)
Hence, TP53 and ARID1A mutations may co-occur in a subset of aggressive or metastatic endometrial cancers, with ARID1A loss promoting squamous differentiation and the acquisition of invasive properties. (nih.gov)
First, we screened 513 natural compounds on primary AML cells and identified a novel diterpene (H4) that preferentially induced differentiation of FLT3 wild-type AMLs, while FLT3-ITD/mutations conferred resistance. (lu.se)
[ 22 ] Investigators transfected a fibroblast cell line with 51 different mutations seen in keratosis follicularis (Darier disease) pedigrees. (medscape.com)

Cell-based therapy using systemic infusion of GMSC in experimental colitis significantly ameliorated both clinical and histopathological severity of the colonic inflammation, restored the injured gastrointestinal mucosal tissues, reversed diarrhea and weight loss, and suppressed the overall disease activity in mice. (ca.gov)
Nevertheless, the effect of GDF-5 on odontoblast differentiation from dental pulp tissues remains unknown. (ispub.com)
For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that the cells be pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. (bdbiosciences.com)
Histological, RT-qPCR, western blot analyses and steroid hormone measurements were performed on in vitro cultured mouse prepubertal testicular tissues and age-matched in vivo controls. (elifesciences.org)
Several fertility restoration approaches, whose aim is to mature cryopreserved tissues in vivo or in vitro in order to produce spermatozoa, are being developed. (elifesciences.org)
The organotypic culture procedure, which preserves testicular tissue architecture, microenvironment and cell interactions, has been used successfully to obtain spermatozoa from fresh or frozen/thawed mouse prepubertal testicular tissues ( 3 - 6 ). (elifesciences.org)
Here, we uncovered a mechanism of progenitor cell allocation that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. (elifesciences.org)
During embryo development, naïve cell lineages undergo concurrent processes of fate specification and morphogenesis as critical steps towards the generation of differentiated tissues and organs. (elifesciences.org)
These early progenitor cells often travel long distances from their induction site to the site of terminal differentiation, making them vulnerable to environmental cues and movement of neighbouring tissues. (elifesciences.org)
Several embryonic tissues and organs originate from small sets of progenitor cells. (elifesciences.org)
T2D results from a reduced ability of the pancreatic beta cells to secrete enough insulin to stimulate glucose utilisation by peripheral tissues. (europa.eu)
The characterization of this protein in vivo has shown that it widely expressed in different tissues and circulates in the blood, its levels rise after food intake in humans and stimulates insulin secretion and sensitivity and inhibits platelet aggregation. (yale.edu)
Local generation of active androgens from circulating androgen precursors is an essential mediator of androgen action in peripheral androgen target cells and tissues. (bham.ac.uk)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

MYH6:mCherry knockin human embryonic stem cell (hESC) reporter line, which allows the identification and purification of SAN-like cells. (stanford.edu)
Understanding the mechanisms underlying embryonic beta cell development will be a prerequisite for the development of a universally available cell replacement therapy in diabetes. (lu.se)

This project seeks to elucidate transcriptional and post-transcriptional regulatory networks that determine cell fate and guide the development of photoreceptors from retinal progenitor cells. (nih.gov)
We are now focused on delineating the transcription factors and signaling pathways that are responsible for generating photoreceptors from retinal progenitor cells. (nih.gov)
Human CD34 + hematopoietic stem and progenitor cells purified from umbilical cord or adult peripheral blood were cultured in a two-phase liquid culture system in a serum-free medium for 21 days. (haematologica.org)
The developmental strategies used by progenitor cells to allow a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. (elifesciences.org)
Despite the importance of the developmental pathways followed by these small groups of progenitor cells and their impact on the physiology of the organism, we still know little about the set of developmental strategies that progenitor cells deploy in vivo to overcome the challenges imposed by the environment as they travel to the site of terminal differentiation. (elifesciences.org)
Downloaded from periodic thymic colonization by progenitor cells guarantees stage. (lu.se)

Activated T-lymphocytes impaired OMSCs via the Fas/Fas ligand pathway, as occur in long bone MSCs. (ca.gov)
It is usually considered that the differentiation process of MSCs depends on specialized external stimulating factors, including cell signaling pathways, cytokines, and other physical stimuli. (wjgnet.com)
Recent findings have revealed other underrated roles in the differentiation process of MSCs, such as material morphology and exosomes. (wjgnet.com)
We investigated the homing-related chemokine expression levels of MSCs treated with a prostate cancer cell line (PC-3) -conditioned medium. (cancerindex.org)
However, effective dose and appropriate time of MSCs delivery are the main challenges in the clinical translation of stem cell therapy. (hindawi.com)

This is mediated by cytokines and other mediators released by the activated immune cells invading the islets, which activate secondary pathways of cell death in the target beta cells. (europa.eu)
One of the most striking features of iNKT cells is usually their ability to rapidly produce multiple cytokines such as IL-4 IFN-γ GM-CSF IL-10 IL-13 and IL-17. (bioskinrevive.com)
These cytokines greatly impact innate immunity shape adaptive immune responses and donate to the defensive and detrimental assignments of iNKT cells in a variety of autoimmune allergic and inflammatory illnesses in protection against microbial an infection and in tumor surveillance (1-5). (bioskinrevive.com)

GMSC can function as an immunomodulatory and anti-inflammatory component of the immune system in vivo and is a promising cell source for cell-based treatment in experimental inflammatory diseases. (ca.gov)
The Parkinson's Disease (PD) kinase LRRK2 is highly expressed in immune cells such as macrophages. (biorxiv.org)
In these cells, LRRK2 regulates innate immune pathways and it is activated after membrane damage leading to the phosphorylation of the Rab GTPases Rab8A and Rab10. (biorxiv.org)
Although some immune-related biomarkers can identify individuals at risk to develop T1D, the process by which the beta cells are destroyed is not well understood. (europa.eu)
Further, in vivo reprogramming may expand on this concept by eliminating the need for manufacture and the risk of immune rejection. (scienceboard.net)
First-generation ex vivo cell therapies, such as autologous anti-CD19 CAR T cells, demonstrated the clinical value and curative potential of enhancing the therapeutic qualities of immune and other patient-derived cell types. (scienceboard.net)
Launch The invariant NKT (iNKT) cells play essential jobs in both innate and adaptive immune system replies (1-4). (bioskinrevive.com)
PD-L1 is expressed mainly on tumor-infiltrating immune cells in roughly 41% of patients with triple negative breast cancer ( 5 ). (frontiersin.org)

In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS. (lu.se)
In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
This also allows us the unique opportunity to directly compare our hESC-derived dopamine neurons with those sourced from human fetal tissue both in vitro and in vivo. (lu.se)

The cross-talk between mGlu3 receptors and BMP receptors was mediated by the activation of the mitogen-activated protein kinase pathway. (nih.gov)

This study aims to investigate the molecular mechanism of Adenovirus type 36 (Ad36) in adipocyte differentiation and glucolipid metabolism. (biomedcentral.com)
Herein, we presented an overview of a previously published work regarding the role of stem cell therapy in ischemic stroke and its underlying molecular mechanisms. (hindawi.com)
An insight into the biology of ischemic stroke indicates that a stream of molecular events initiates instantly after the onset of ischemic stroke, such as oxidative stress, increased level of intracellular calcium, excitotoxicity, and inflammation which results in apoptotic or necrotic neuronal cell death [ 12 - 14 ]. (hindawi.com)
In conclusion, a reduction in beta cell mass is a key component of diabetes mellitus and the molecular mechanisms underlying beta cell loss remain to be clarified. (europa.eu)
These findings validate the proposed systems biology approach to understand the molecular pathways underlying decreased beta cell mass in diabetes mellitus and have provided important information for the development of novel alternatives to preserve beta cell mass in early diabetes. (europa.eu)
The derived hESC-SAN cells display molecular and electrophysiological characteristics of bona fide nodal cells, which allowed exploration of their transcriptional profile at single-cell level. (stanford.edu)
We combine in vivo genetic mouse models with in vitro tissue culture and molecular biology techniques to better understand the role of specific signaling pathways and transcription factors in regulation of bone and joint development as well as in the onset and progression of osteoarthritis. (rochester.edu)
[ 3 ] In an attempt to improve outcomes in HNSCC, most research in the field is now focusing on the molecular biology of HNSCC in an attempt to target select pathways involved in carcinogenesis. (medscape.com)
With the increased understanding of molecular mechanisms and basic pathways in the pathogenesis of squamous cell cancer of the head and neck , these pathways may be modified, and rational approaches in cancer therapy at the molecular level may be created. (medscape.com)
The goal of specific molecular targets in cancer therapy is to create a "magic bullet" that selectively kills cancer cells. (medscape.com)
As our understanding of the molecular biology of HNSCC continues to develop, we can target the specific components of cancer cells that are not found in normal cells. (medscape.com)
Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)

2015) . Jarid2 methylation via the PRC2 complex regulates H3K27me3 deposition during cell differentiation . (sdbonline.org)

This Specific Targeted Research Project (STREP) aims to utilise functional genomics to identify pathways responsible for the reduction of beta cell mass in diabetes, and use this knowledge to define targets for intervention to preserve beta cell mass. (europa.eu)
Here as a complement to genetic profiling, we used unbiased, combinatorial in vitro drug screening to identify pathways that drive AML and to develop personalized combinatorial treatments. (lu.se)

To transfer to the Sykiotis lab the 3D follicle model established by the Costagliola lab in order to perform cell-based studies on the role of stress response pathways on iodine metabolism. (unil.ch)

Prior to the invention of CAR, cell therapies were largely limited to allogeneic hematological transfusion and transplantation. (scienceboard.net)
Therefore, a central goal of diabetes research is to regenerate pancreatic beta cells or produce large numbers of functional beta cells for transplantation into diabetic patients. (lu.se)
We monitor our cells after transplantation using standard in vivo assessments as listed above. (lu.se)

What are intrinsic control mechanisms that lead to photoreceptor cell fate from retinal progenitors? (nih.gov)
We previously discovered that that Maf-family bZIP transcription factor NRL is critical for rod photoreceptor fate and functional differentiation, and that loss of NRL leads to S-cones instead of rods. (nih.gov)
The remaining delaminated cells follow the movement of apically attached progenitors by a protrusion-dependent cell-cell contact mechanism, avoiding sequestration by the adjacent endoderm, ensuring their collective fate and allocation at the site of differentiation. (elifesciences.org)

Using mice and traditional 2D cell cultures, new factors have been identified that mediate responses of thyroid cells to various forms of stress encountered both during normal physiology of the thyroid gland and also during extreme conditions associated with thyroid disorders. (unil.ch)
To investigate in parallel the functions of these pathways in mice and in zebrafish using genetic means. (unil.ch)
While clusters of tumour cells were consistently found in the brain of control mice, they were virtually absent in a large proportion of mice treated with LY341495. (nih.gov)
Mice treated with anti-miR-135b showed well-differentiated tumours with an acinar pattern whereas tumours in the control groups showed poor differentiation and adenomatous pattern. (gla.ac.uk)
intervention in the defective signal transduction pathways identified above using genetically modified mice, long-acting viral vectors and small interfering RNAs. (europa.eu)
It was shown that these mice fail to increase their beta cell mass in response to a high fat diet and develop diabetes. (europa.eu)
Though it was reported that TSC1-deficient mice contain reduced iNKT cells. (bioskinrevive.com)
and in vivo models of corneal wound healing in wild type, Sphk1, and S1P3 knockout mice, along with testing the therapeutic potential of targeting S1P and TGF-ß signaling using selective inhibitors in these models (Aim 2). (hhs.gov)
Nude mice implantation tumor model was applied to observe the effect of CHAG on implantation tumor of OC cells. (bvsalud.org)
Here, through the use of conditional knock-out mice, we demonstrate a requirement for ILK in oligodendrocyte differentiation and axonal myelination in vivo . (jneurosci.org)
We used genetically engineered mice and in vivo genomic approaches to discern both unique and overlapping roles of TP53 and ARID1A in the endometrium. (nih.gov)
Knockout of Timp1 in mice caused a significant reduction in fibrotic focus formation, collagen fiber deposition, recruitment of fibroblasts, and differentiation of fibroblasts into myofibroblasts in the lungs, indicating that TIMP1 plays a critical role in the pulmonary fibrotic response to MWCNTs. (cdc.gov)

The emergence of functional studies on animal lectins during the 1990s has provided the appropriate framework to better understand their roles in cell death. (nature.com)
The Sykiotis lab has been studying the roles of the antioxidant response pathway in the thyroid gland in health and disease. (unil.ch)
In addition, the developmental roles and the evolutionary conservation of the antioxidant response pathway in the thyroid remain unknown. (unil.ch)

In this context, we have outlined a project with the overall goal to combine the respective strengths and expertise of the two labs to elucidate the physiological and developmental functions and evolutionary aspects of the stress response pathway in the thyroid. (unil.ch)
The clinical success of anti-CD19 CAR T-cell therapies has not only provided efficacious treatment alternatives for cancer patients but also aided the developmental and regulatory precedent for the translation of cell-based therapies for other disease areas. (scienceboard.net)
As a result, developmental trends have shifted toward an allogeneic approach, seeking to replace autologous cell sources with consistent and scalable alternatives, such as iPSCs. (scienceboard.net)

To achieve this ambitious goal, we have established a consortium of leading European experts from seven European countries in the fields of pancreatic beta cell diabetes research, functional genomics and bioinformatics. (europa.eu)
While expanding our knowledge of graft integration, we also investigate how that occurs at a functional level by utilising optogenetics in combination with highly sensitive and quantitative methods assessing neuronal function, such as electrophysiology and in vivo amperometry. (lu.se)
[ 7 , 8 ] As background, in 2009, Collombat and colleagues [ 9 ] showed that pancreatic misexpression of the gene Pax4 led to the conversion of glucagon -producing alpha cells into functional beta cells. (medscape.com)

T1D is characterised by a near complete lack of insulin production due to specific destruction of the pancreatic beta cells that typically develops over several years. (europa.eu)

What pathways are this gene/protein implicaed in? (cancerindex.org)
The antitumor efficacy was studied in mouse mammary carcinoma models (Her2/neu transgenic and 4T1-luc mammary cancers) treated with systemic recombinant protein with/without the depletion of myeloid-derived suppressor cells or intra-tumoral gene electrotransfer (GET). (frontiersin.org)
The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)
protein patterns of subjects with leish- Cell culture maniasis [17]. (who.int)

Other inhibitory pathways include SOCS proteins and O-GlcNAcylation of IRS-1. (wikipedia.org)
Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)

In the adult, HSC quiescence, maintenance, and differentiation are closely linked to changes in metabolism. (lu.se)
In contrast, OXPHOS-mediated differentiation of HE toward definitive hematopoiesis is dependent on cholesterol metabolism. (lu.se)
Our findings reveal that during EHT, metabolism is a major regulator of primitive versus definitive hematopoietic differentiation. (lu.se)
However, the mechanism underlying the effect of Ad36 induced adipocyte differentiation and changes in glucose and lipid metabolism is unclear. (biomedcentral.com)
We have developed expertise in in vivo investigation of lipid and glucose metabolism, insulin secretion and sensitivity, and vascular biology and in human physiological studies, leading to discovery of attractive drug targets that have been either patented or being investigated for their utility in treatment of fatty liver disease and diabetes in 2 clinical trials in the outlier populations of Fars/Iran. (yale.edu)

Deletion of Jarid2 leads to impaired orchestration of gene expression during cell lineage commitment. (sdbonline.org)

Functions of RARs and RXRs in vivo: Genetic dissection of the retinoid signaling pathway" Pure and Applied Chemistry , vol. 75, no. 11-12, 2003, pp. 1709-1732. (degruyter.com)
In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
The goal of gene therapy is to introduce new genetic material into cancer cells that selectively kills them without causing toxicity to the surrounding cells. (medscape.com)
Th)1 cells.5 Meanwhile, the function and immunity of with genetic predisposition, involving multiple cells, DCs are closely regulated by miRNAs. (cdc.gov)

Furthermore, MWCNTs elicited a significant induction of CD63 and integrin B1 in lung fibroblasts, leading to the formation of a TIMP1/CD63/integrin B1 complex on the surface of fibroblasts in vivo and in vitro, which triggered the phosphorylation and activation of Erk1/2. (cdc.gov)

In the context of either TP53 or ARID1A loss, PIK3CA mutant endometrium exhibited inflammatory pathway activation, but other gene expression programs differed based on TP53 or ARID1A status, such as epithelial-to-mesenchymal transition. (nih.gov)
2012) . Sterile inflammation associat- The key mechanistic pathways and vation of inflammatory cel s (Balkwill, ed with inhalation of crystal ine silica mediators involved in inflamma- 2012 ). (who.int)

IRS-1 is required for maintenance of neoplasmic phenotype in adenomatous polyposis coli (APC) - mutated cells, it is also needed for transformation in ectopically expressing oncogenic β-catenin cells. (wikipedia.org)
One of the main challenges with bioprinting cell laden constructs is the identification of an appropriate bioink, as the material not only needs to have the necessary structural and mechanical properties, but should also protect the cells from damage during printing and ultimately provide them with an appropriate environment to direct or control their phenotype and function. (nature.com)
Moreover, intimal SVG SMCs displayed a decrease in myosin heavy chain (MYH)11 and a trend towards a decrease in calponin expression compared to medial SMCs, suggesting in vivo SMC phenotype switching. (gla.ac.uk)
The observation of in vivo SMC phenotype switching in pre-implantation SVGs prompted development and generation of a ligand-independent contractile differentiation protocol for primary HSVSMCs and primary human coronary artery (HCA) SMCs. (gla.ac.uk)
MicroRNAs not only participate in determining DCs phenotype and then naive T lymphocyte differentiation, but also participate in the regulation of airway inflammation and airway remodeling in asthma. (cdc.gov)

The responding samples to H4, displayed increased expression of myeloid markers, a clear decrease in the nuclear-cytoplasmic ratio and the potential of re-activation of the monocytic transcriptional program reducing leukemia propagation in vivo. (lu.se)
Previously, our research team had found that two molecules, or more specifically, co-transcriptional regulators, known as YAP and TAZ, are involved in the damage of the lung epithelial cells. (lu.se)

To transfer to the Costagliola lab expertise on the Nrf1 and Nrf2 pathways and their manipulation with pharmacological compounds. (unil.ch)

Glycosylation of classical death receptors fine-tunes cell death programs. (nature.com)
In conclusion, CHAG had inhibitory effect on tumor cells, through covering cell surface and blocking the interaction between extracellular stimulative factors and their receptors. (bvsalud.org)

However, metabolic processes underlying the emergence of HSCs from HE cells remain unclear. (lu.se)
RUNX3 , STAT4 , NFKB1 ) and metabolic pathways (e.g. (biomedcentral.com)

In vitro systems are used to differentiate these cells into diverse macrophage subsets to mimic the populations observed in vivo . (biorxiv.org)
During development, oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs) that extend processes to contact multiple axons of CNS neurons. (jneurosci.org)

We are using state-of-the-art next generation sequencing combined with bioinformatic strategies, and developing stem cell-based approaches for gene therapy and drug discovery. (nih.gov)
Targeted-therapies enhancing differentiation of glioma-initiating cells (GICs) are potential innovative approaches to the treatment of malignant gliomas. (nih.gov)

Here we show that 32D cells transformed with two different oncogenes, v-src or c-fms [S301,F969], both belonging to the CSF-1 transduction pathway, respond to exogenous wt-p53 expression with the same final outcome-monocytic differentiation. (mcmaster.ca)

Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
The answers to these questions will be valuable for delineating pathogenic mechanisms that contribute to photoreceptor cell death. (nih.gov)
Zaniar's work is focused on identifying the mechanisms of cardiac arrhythmias using several experimental systems ranging from genetically engineered animal models to human pluripotent stem cell derived cardiac cell types. (stanford.edu)

To address some of the limitations of these models, here we used two complementary ex vivo models based on Xenopus embryos: pluripotent animal cap explants expressing Gata4 transcription factor and conjugates of gastrula -stage AE with animal caps (AC). (xenbase.org)

Liver Specification in the Absence of Cardiac Differentiation Revealed by Differential Sensitivity to Wnt/β Catenin Pathway Activation. (xenbase.org)
It also delivers its function without activating regulatory T cells (Tregs) ( 9 ) and mediating activation-induced cell death of effector cytotoxic T cells ( 10 ). (frontiersin.org)
To examine the activation of classic and 11-oxygenated androgens within human peripheral blood mononuclear cells (PBMCs), PBMCs were isolated from healthy donors and subsequent activity analysis assays were carried out. (bham.ac.uk)

Immunoreceptor signaling during development, homeostasis, and effector function of T cells and NK cells. (upstate.edu)

Although it is becoming apparent that TSC1/mTOR signaling is normally involved with many areas of T cell biology the need for TSC1/mTOR in iNKT cells is normally unclear. (bioskinrevive.com)

1 The accumulation of free excess α-globin chains in red blood cells and their precursors, as a result of the decreased production of β-globin, is believed to be the main pathophysiological mechanism leading to hemolytic anemia and ineffective erythropoiesis in β-thalassemia. (haematologica.org)
T-cell acute lymphoblastic leukemia (T-ALL) arises from leukemic transformation and expansion of immature T-cell precursors. (aacrjournals.org)
PBMCs were incubated ex vivo with androgen precursors and active androgens from the classic pathway (DHEA, A4 and T) and the 11-oxygenated androgen pathway (11KA4, 11KT, 11OHA4 and 11OHT). (bham.ac.uk)

We demonstrate that glycolysis-mediated differentiation of HE toward primitive erythroid hematopoiesis is dependent on the epigenetic regulator LSD1. (lu.se)
Our data show that selective silencing of α-globin expression in erythroid cells is pharmacologically feasible, and IOX1 is a lead compound to developing new therapy to treat β-thalassemia through the novel pathway of downregulating α-globin expression. (haematologica.org)
We first optimized a serum-free, miniature erythroid differentiation system starting from primary human CD34 cells, the exact type of cells we would ultimately like to target in vivo ( Figure 1 ). (haematologica.org)
Characterization and validation of the small scale erythroid differentiation system used for small molecule screen. (haematologica.org)

Developing next-generation cell therapies centers around the enhancement of efficacy, safety, and accessibility. (scienceboard.net)

miR-135b silencing in vivo reduced tumour multiplicity and tumour load in the AOM/DSS colorectal cancer model. (gla.ac.uk)
The in-vivo silencing of miR-135b shows preclinical efficacy with low toxicity and represents the first in-vivo study for use of anti-miRs in treatment of colorectal cancer. (gla.ac.uk)

Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. (bdbiosciences.com)

ES cells are pluripotent cells, which mean that they can give rise to all differentiated cell types derived from all three germ layers. (bvsalud.org)

This study demonstrated clinical and experimental evidences supporting a potential efficacy and safety of utilizing autologous PDL cells in the treatment of human periodontitis. (ca.gov)
Efficacy of radiation and temozolomide was examined in vitro and in an allograft model in vivo. (nih.gov)
The goal of this study was to examine the activity of IL-15/IL-15Rα complex (IL-15cx) to CD8 + T cells and evaluate its potential efficacy in murine breast cancer models. (frontiersin.org)
[ 3 ] Clinical trials are currently under way on the safety, tolerability, and efficacy of transplanting such cells in encapsulated forms in persons with type 1 diabetes, with results to be announced in the coming years. (medscape.com)

Due to their wide-range of functions in immunity and tissue remodelling, macrophages in vivo are phenotypically heterogeneous. (biorxiv.org)
Deregulation of mTOR signaling because of TSC1 deficiency continues to be implicated in propensity to loss of life lack of quiescence and level of resistance to anergy of T cells aswell as unusual function of mast cells and macrophages (34-41). (bioskinrevive.com)

Anatomy65

Organisms8

Diseases8

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes57

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science4

Genes15

Induce7

Induces6

Proliferation12

PI3K6

Regeneration7

Mesenchymal stem5

Maturation6

Apoptosis6

Cellular11

Stem cells33

Inhibitor5

Multi-lineage differentiation1

Growth26

Receptor6

Mutations7

Tissues14

Embryonic2

Progenitor cells6

MSCs5

Cytokines3

Immune8

Vitro and in vivo3

Kinase pathway1

Molecular12

Regulates1

Identify pathways2

Response pathways1

Transplantation3

Fate3

Mice12

Roles3

Developmental3

Functional3

Pancreatic beta cells1

Protein4

Proteins4

Metabolism5

Lineage commitment1

Genetic4

Fibroblasts1

Inflammatory2

Phenotype5

Transcriptional2

Pharmacological1

Receptors2

Metabolic2

Differentiate2

Approaches2

Monocytic1

Mechanisms3

Transcription1

Activation3

Effector1

Biology1

Precursors3

Erythroid4

Therapies1

Colorectal cancer2

Characterization1

Pluripotent1

Efficacy4

Macrophages2