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  • murine
  • Yet, N-CAM is also expressed by T cell subsets, muscle cells, and neurons, but it is not expressed by murine NK cells ( 9 ). (pnas.org)
  • Ly49H is an NK cell-specific activating receptor that accounts for the genetic resistance to murine CMV (MCMV). (jimmunol.org)
  • In addition, to explore the in vivo relevance of targeting PGE2 to reduce MDSC-mediated suppression of NK cells, we established a murine model, where tumor cells were disabled from cyclooxygenase-2 (COX-2) production. (aacrjournals.org)
  • Furthermore, silencing COX-2 in murine 4T1 tumor cells reduced the accumulation of CD11b + Gr1 + MDSCs in the spleen, resulting in concomitant improved in vivo clearance of NK-cell sensitive YAC-1 cells. (aacrjournals.org)
  • The first demonstration of antigen-specific recall responses by NK cells was in the setting of hapten-induced contact hypersensitivity, where CXCR6 + liver-derived murine NK cells could mediate antigen-specific contact hypersensitivity independently from B and T cells [ 13 , 14 ]. (hindawi.com)
  • We examined the role of glycolysis in NK cell function during murine cytomegalovirus (MCMV) infection and the ability of IL-15 to prime NK cells during CMV infection. (jci.org)
  • while, more recently, we employed validated murine models of surgical stress and spontaneous metastases [ 11 ] to provide in vivo evidence of global NK cell dysfunction in postoperative metastatic disease. (springer.com)
  • tumor cells
  • CD69−/− mice showed an enhanced NK cell-dependent response that leads to a higher protection and rejection of MHC class I − tumor cells compared with wild-type (WT) mice. (rupress.org)
  • receptor
  • NKp46/CD335 is an Ig-like superfamily cell surface receptor involved in human NK cell activation. (pnas.org)
  • Using NKp46 promoter, we generated transgenic mice expressing EGFP and the diphtheria toxin (DT) receptor in NK cells. (pnas.org)
  • Thus, continuous activating receptor engagement can induce a transient tolerance in mature NK cells in vivo. (jimmunol.org)
  • The Ly49H activation receptor, which is expressed on a subset of NK cells in C57BL/6 mice, is coupled to the DAP12 adaptor protein. (jimmunol.org)
  • ALT-803 is a superagonist IL-15 mutant and IL-15Rα-Fc fusion complex that activates the IL-15 receptor constitutively expressed on natural killer (NK) cells. (aacrjournals.org)
  • additionally, NK cells are potently activated by CD16 or Fc γ RIIIa (low-affinity Fc receptor for IgG)-dependent recognition of IgG-opsonized targets. (hindawi.com)
  • AIM/CD69 is the earliest activation cell surface receptor on leukocytes. (rupress.org)
  • The CD1d reactive glycolipid, α-galactosylceramide (α-GalCer), potently activates T cell receptor-α type I invariant NKT cells that secondarily stimulate the proliferation and activation of other leukocytes, including NK cells. (rupress.org)
  • mediate
  • However, IL12 possesses a unique potential for immunocytokine development because of its ability to mediate a potent CD4 + T-cell response and because of its benign effect at sites of inflammation, making IL12-based immunocytokines particularly suited for the treatment of polymorbid patients. (aacrjournals.org)
  • 1 In contrast, NK cells reconstituting after transplantation can overcome this major histocompatibility complex barrier by KIR ligand mismatching to mediate a potent anti-leukemia reaction by decreased triggering through inhibitory KIR. (bloodjournal.org)
  • Abstract
  • From the publication abstract, the clinical trial demonstrated, "production feasibility and safety of infusing high doses of ex vivo expanded NK cells after haploidentical HSCT without adverse effects, increased GVHD or higher mortality, and was associated with significantly improved NK cell number and function, lower viral infections, and low relapse rate post-transplant. (cytosen.com)
  • lymphoid
  • NK cells are present in lymphoid organs as well as in nonlymphoid peripheral tissues ( 2 ). (pnas.org)
  • The results of this study indicate that PDT is a highly effective means of generating tumor-sensitized immune cells that can be recovered from lymphoid sites distant to the treated tumor at protracted time intervals after PDT, which asserts their immune memory character. (aacrjournals.org)
  • Due to the lack of phenotypically detectable lymphoid cells, it was necessary to address the question of the lymphoid potential of the expanded populations under these conditions. (irsn.fr)
  • CONCLUSION: This culture system permits the revelation of a population that, although poorly represented in terms of phenotypically detectable cells, nevertheless retains high levels of lymphoid NK and B potential after 14 days expansion. (irsn.fr)
  • Such data suggest the persistence, or expansion, of lymphoid progenitors and, hence, the multipotentiality of the expanded progenitor/stem cells. (irsn.fr)
  • IL-21 has pleiotropic roles in the lymphoid lineages, including the promotion of CD8 + effector cell function ( 19 ) and the inhibition of IgE production in B cells ( 20 , 21 ). (rupress.org)
  • therapeutic
  • Strategies are emerging to apply NK cells as therapeutic agents against a broad range of malignancies ( 6 , 7 ). (pnas.org)
  • Along with highlighting these issues, we speculate that memory NK cell-based adoptive immunotherapy settings would greatly take advantage from the combination with tumor-targeting therapeutic antibodies (mAbs), as a strategy to fully unleash their clinical efficacy. (hindawi.com)
  • These results indicate that G-MDSCs can negatively regulate NK cell activation and function in response to VV infection and suggest that manipulation of G-MDSCs could represent an attractive strategy for regulating NK cell activities for potential therapeutic benefits. (jimmunol.org)
  • haploidentical
  • Depletion of host regulatory T cells with IL2DT improves efficacy of haploidentical NK cell therapy for refractory acute myeloid leukemia. (bloodjournal.org)
  • Haploidentical natural killer (NK) cell infusions can induce remissions in some patients with acute myeloid leukemia (AML) but regulatory T-cell (Treg) suppression may reduce efficacy. (bloodjournal.org)
  • 3 Patients were treated with lymphodepleting chemotherapy and received haploidentical NK cell infusions from siblings, parents, or children, followed by subcutaneous interleukin (IL)-2 to stimulate NK proliferation and activation. (bloodjournal.org)
  • maturation
  • Within the NK cell lineage, IL-21 was reported to enhance NK cell maturation from human multipotent bone marrow progenitors and to activate peripheral NK cells in the absence of other stimuli ( 16 ). (rupress.org)
  • Immunology
  • Veerle De Colvenaer , Sylvie Taveirne UGent , Maarten Delforche , Magda De Smedt , Bart Vandekerckhove UGent , Tom Taghon UGent , Louis Boon , Jean Plum and Georges Leclercq UGent ( 2011 ) IMMUNOLOGY AND CELL BIOLOGY . (ugent.be)
  • adaptive
  • The perspective of NK cells as exquisitely innate effectors is challenged by the recent appreciation that NK cells can adapt their functional program in response to environmental factors, through the generation of long-lasting specialized NK cell populations with enhanced effector functions, named adaptive or memory NK cells [ 10 - 12 ]. (hindawi.com)
  • Ex Vivo Expanded Adaptive NK Cells Effectively Kill Primary Acute Lymphoblastic Leukemia Cells. (nih.gov)
  • Donor
  • We present preliminary data suggesting that B-CLL susceptibility significantly correlates with HLA mismatch between NK cell donor and B-CLL patient. (frontiersin.org)
  • hematopoietic stem cell trans
  • Researchers from multiple institutions, including Drs. Stefan Ciurea and Dean Lee, published data last month showing the feasibility and safety for preventing relapse of acute myeloid leukemia (AML) by treating with ex vivo expanded NK cells in combination with haploindentical hematopoietic stem cell transplantation (HSCT). (cytosen.com)
  • monocytes
  • Suppression of NK-cell activity by PGE2-treated monocytes was compared with that of freshly isolated CD14 + HLA-DR low/− monocytic MDSCs (moMDSC) from patients with melanoma. (aacrjournals.org)
  • phenotypic
  • However, our understanding of NK cell biology is severely limited by the lack of consensus phenotypic definition of these cells across species, by the lack of specific marker to visualize them in situ , and by the lack of a genetic model where NK cells may be selectively ablated. (pnas.org)
  • In addition to human, we show here that NKp46 is expressed by NK cells in all mouse strains analyzed, as well as in three common monkey species, prompting a unifying phenotypic definition of NK cells across species based on NKp46 cell surface expression. (pnas.org)
  • Second, there is no consensus phenotypic definition of NK cells across species. (pnas.org)
  • These various phenotypic NK cell definitions and the lack of specific NK cell markers impede the comparison of data across species. (pnas.org)