Vivo imaging optical imaging. Medical search. Frequent questions

Furthermore, the total fluorescence emanating from the assemblies gives rise to a localized optical signal of sufficient intensity to penetrate through the dense tumor tissue of a live animal. (duke.edu)
Robust NIR-emissive polymersomes thus define a soft matter platform with exceptional potential to facilitate deep-tissue fluorescence-based imaging for in vivo diagnostic and drug-delivery applications. (duke.edu)
In vivo fluorescence was strongly observed at 48 hr over the head, especially with removal of both head skin and skull bone. (elsevierpure.com)
The in vivo Cy5.5 fluorescence representing A5 signal spatially surrounding the ischemic core temporally detects an early-stage apoptosis after cerebral ischemia. (elsevierpure.com)
Medical imaging techniques such as fluorescence image-guided surgery often make use of the NIR window to detect deep structures. (wikipedia.org)
A) Fluorescence-advanced videodermatoscopy image shows oval body with 3 pairs of legs. (cdc.gov)
However, due to some inherent limitations of current Fd-OCT technology (e.g., lack of spectroscopic information, inability to measure fluorescent signals), it is important to co-register Fd-OCT data with images obtained by other clinical imaging modalities such as fundus cameras and fluorescence angiography to create a more complete interpretation and representation of structures imaged. (spie.org)
But fluorescence intensity of QDs accumulated in the tumors lasts longer, allowing her to image tumor cells. (the-scientist.com)
This includes facilitating imaging with fluorescence, bioluminescence and radioisotopes. (bruker.com)
He will also highlight how the Bruker In Vivo Xtreme II imaging system combines four imaging modalities - luminescence, fluorescence, radioisotope imaging and X-ray - in one to deliver an unparalleled level of sensitivity, speed and versatility in preclinical imaging. (bruker.com)
His research focuses on biomedical photonics, and he has specific expertise in fluorescence imaging and spectroscopy, optical coherence tomography (OCT), and multiphoton endomicroscopy for cancer detection, surgical guidance, and preterm-birth risk assessment etc. (hopkinsmedicine.org)
Noninvasive near infrared (NIR) fluorescence imaging provides visualization and detection of full body tissue bio-distribution of a new drug in real-time. (grandviewresearch.com)
Fluorescence microscopes and live cell imaging for microbiology at Dept. of Biology. (lu.se)
Live Cell Imaging Platform at MultiParkNikon Ti-E is a fully motorised research microscope with Spectra X light engine and EMCCD, suitable for imaging of fluorescence labelling, such as DAPI, CFP, GFP, YFP, mCherry and Cy5. (lu.se)
Plate Runner Platform at MultiParkHigh content screening (HCS) / Analysis (HCA) enables automated acquisition, visualization and quantification of a large number of images such as rapid snapshot acquisition of fluorescence images of cells and small organisms. (lu.se)
Cellomics Platform at MultiParkThe Cellomics ArrayScan VTI HCS Reader enables automated acquisition, visualization and quantification of a large number of images, with an automated fluorescence and brightfield microscopy allowing extraction of quantitative data from intensity and area measurement in high-throughput format. (lu.se)
The SIM-E is a fluorescence super-resolution microscope, the Ti2 is a fluorescence microscope for live imaging of pathogens, BSL2-compliant and the TE-300 is a fluorescence wide-field microscope. (lu.se)
The B10 set-up consists of a HEKA EPC10 double-patch clamp amplifier, an Olympus BX51WI microscope with multi-channel fluorescence capability and differential interference contrast for live imaging and optical manipulation of cells (e.g. optogenetics and calcium imaging), and a Hamamatsu C11440-10C camera. (lu.se)

In: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVI. (kent.ac.uk)
A miniature endoscope probe for forward viewing in a 50 kHz swept source optical coherence tomography (SS-OCT) configuration was developed. (kent.ac.uk)
Liu Y, Ai K, Lu L. Nanoparticulate X-ray computed tomography contrast agents: from design validation to in vivo applications. (springer.com)
Computed tomography imaging of primary lung cancer in mice using a liposomal-iodinated contrast agent. (springer.com)
Ai K, Liu Y, Liu J, Yuan Q, He Y, Lu L. Large-scale synthesis of bi 2S 3 nanodots as a contrast agent for in vivo X-ray computed tomography imaging. (springer.com)
Liu Y, Ai K, Liu J, Yuan Q, He Y, Lu L. A high-performance ytterbium-based nanoparticulate contrast agent for in vivo X-ray computed tomography imaging. (springer.com)
In this context, Animascope was founded to provide state-of-the-art multimodality imaging services to international customers: optical imaging, ultrasound imaging (US), X-ray computed tomography (CT), positron emission tomography (PET), single photon emission computed tomography (SPECT) and such as magnetic resonance imaging (MRI). (pharmaceutical-business-review.com)
There are five imaging modalities available for molecular imaging, including X-ray computed tomography imaging (CT), optical imaging (OI), radionuclide imaging (involving PET and SPECT), ultrasound (US) imaging and magnetic resonance imaging (MRI) [ 9 ]. (hindawi.com)
The purpose of this study is to demonstrate the application of ultrahigh-resolution Spectral Domain Optical Coherence Tomography (SD-OCT) for non contact in vivo imaging of the retina of birds of prey, which to the best of our knowledge has never been attempted. (spie.org)
Ultra-high isotropic resolution imaging of retinal structures was made possible with an adaptive optics system using dual deformable mirrors and a Fourier-domain optical coherence tomography (Fd-OCT) system with correction for longitudinal chromatic aberration. (spie.org)
Advances in Fourier-domain optical coherence tomography (Fd-OCT) permit visualization of three-dimensional morphology of in-vivo retinal structures in a way that promises to revolutionize clinical and experimental imaging of the retina. (spie.org)
Optical projection tomography can image embryos at high resolution, but of fixed specimens only. (the-scientist.com)
Magnetic resonance imaging and optical coherence tomography feature noninvasive imaging, but do not easily provide specific contrasts. (the-scientist.com)
Optical coherence tomography: advanced technology for the endoscopic imaging of Barrett's esophagus," Endoscopy 32(12):921-930 (2000). (hopkinsmedicine.org)
In comparison, computed tomography (CT) is superior in lesion depiction and characterization and is the preferred imaging modality in the initial workup of pancreatic lesions. (medscape.com)
Multispectral Optoacoustic Tomography is a powerful optoacoustic imaging system for small animal imaging. (lu.se)
Alexander Bengtsson, Material and technique development for ultrasound optical tomography using spectral hole burning filters , PhD thesis, Faculty of Engineering, Lund University 2022. (lu.se)

HAIP offers services in various animal imaging modalities. (helsinki.fi)
Here, we summarize the imaging modalities used for image-guided therapies and discuss recent advances on nanoparticle-based image-guided therapies. (springer.com)
A molecular imaging primer: modalities, imaging agents, and applications. (springer.com)
Combining preclinical in vivo imaging modalities offers the opportunity to synergize the strengths of each in one experiment. (bruker.com)
Different types of in-vivo modalities are used in small animal imaging operations. (grandviewresearch.com)
PET/CT, PET/MRI, and SPECT/CT are the recent technological advancements in preclinical in-vivo modalities. (grandviewresearch.com)
These non-invasive modalities allow functional, structural, and molecular imaging of subjects. (grandviewresearch.com)
Optical imaging was the largest segment in terms of revenue in 2016 owing to its rapid and easy operations and better cost-effectiveness as compared to other modalities. (grandviewresearch.com)

Traditional PA imaging systems employ expensive and bulky class IV lasers with low pulse repetition rate, due to which its availability for preclinical cancer research is hampered. (mdpi.com)
Animascope experts have several years of experience in discovery and preclinical imaging in vivo. (pharmaceutical-business-review.com)
Preclinical imaging technologies support the development of innovative diagnostic, prognostic and therapeutic applications. (pharmaceutical-business-review.com)
Preclinical imaging studies are therefore of great interest for the development of new pharmaceuticals through the use of a broad range of existing methodologies and the development of new diagnostic and therapeutic options for the management of pathologies. (pharmaceutical-business-review.com)
In biomedical research, non-invasive in vivo imaging is applicable to exploratory research and bridges the gap between preclinical and clinical research. (pharmaceutical-business-review.com)
Preclinical medical imaging is applicable to dynamic distribution and biomarker validation studies, and assessment of the delivery, liberation, efficacy and safety of drugs, biologics, biopharmaceuticals, nanoparticles, biomaterials and stem/progenitor cells. (pharmaceutical-business-review.com)
New imaging technologies will ultimately contribute to identify important biomarkers and surrogate endpoints both for preclinical and clinical research. (pharmaceutical-business-review.com)
To achieve this milestone, it must first be established that CLI data correlate to PET data in an in vivo preclinical antitumor study. (snmjournals.org)
Moreover, there is increasing interest in the role of medical imaging for both clinical and preclinical research and biomarker qualification ( 3 - 6 ). (snmjournals.org)
To facilitate the translation of imaging biomarkers from research to development, many preclinical imaging facilities use a multimodal approach, wherein the strengths of each individual modality can be combined and leveraged for the development of robust assays ( 13 , 14 ). (snmjournals.org)
Due to its advantages of high sensitivity and quantifiability, radionuclide molecular imaging plays an important role in clinical and preclinical researches [ 11 ]. (hindawi.com)
A lot of preclinical and clinical studies have confirmed the feasibility of using radionuclide molecular imaging to detect tumor and predict response to therapy [ 12 , 13 ]. (hindawi.com)
Co-presenter Dr. Andrew Van Praagh, Field Applications Scientist, from Bruker BioSpin, will demonstrate some specific examples of preclinical research drawing together the technology from both companies, including Bruker's multimodal Xtreme II imaging system. (bruker.com)
Since 2014, he has served as Lab Manager for the Preclinical Imaging Lab at Bruker BioSpin. (bruker.com)
The M2 3D MR-based histology system is a compact, multimodal, and high-resolution MRI instrument for high-throughput in-vivo and ex-vivo imaging of preclinical subjects. (grandviewresearch.com)
Increasing investments of CROs in preclinical studies and rising usage of multi-functional pre-clinical imaging are poised to provide a fillip to the market. (grandviewresearch.com)

With a combination of near-infrared spectral properties, enhanced optical properties,and significantly improved biosafety profile, this novel hybrid nanoparticulate fluorophore system demonstrably provides real-time, deep-tissue fluorescent imaging of live animals, laying a foundation for further development toward clinical application. (nih.gov)
Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. (lu.se)
article{fb3af781-e293-42a0-8bc7-b112dd9b3836, abstract = {{Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. (lu.se)
Furthermore, we demonstrate the imaging capability of the probe by obtaining B-scan images of diseased larynx tissue and compare them with those obtained in a 1310 nm SS-OCT classical non-endoscopic system. (kent.ac.uk)
The near-infrared (NIR) window (also known as optical window or therapeutic window) defines the range of wavelengths from 650 to 1350 nanometre (nm) where light has its maximum depth of penetration in tissue. (wikipedia.org)
Optical scattering occurs due to mismatches in refractive index of the different tissue components, ranging from cell membranes to whole cells. (wikipedia.org)
These include intravital and multiphoton imaging of mice, optical and US imaging from rodents to larger animals, SPECT/CT imaging of rodents and and PET imaging of larger animals, including production of the required radiotracer, MRI imaging of larger animals and micro-CT of tissue microstructures and rodents. (helsinki.fi)
Heart tissue can be imaged in real-time during keyhole procedures using a new optical ultrasound needle. (sciencedaily.com)
Heart tissue can be imaged in real-time during keyhole procedures using a new optical ultrasound needle developed by researchers at UCL and Queen Mary University of London (QMUL). (sciencedaily.com)
Doctors currently rely on external ultrasound probes combined with pre-operative imaging scans to visualise soft tissue and organs during keyhole procedures as the miniature surgical instruments used do not support internal ultrasound imaging. (sciencedaily.com)
The optical ultrasound needle is perfect for procedures where there is a small tissue target that is hard to see during keyhole surgery using current methods and missing it could have disastrous consequences," said Dr Malcolm Finlay, study co-lead and consultant cardiologist at QMUL and Barts Heart Centre. (sciencedaily.com)
Reflections of these ultrasonic pulses from tissue are detected by a sensor on a second optical fibre, giving real-time ultrasound imaging to guide surgery. (sciencedaily.com)
We used a custom, fast tissue-clearing protocol based on a high refractive-index aqueous solution to increase the imaging depth and allow us the detection of faint signals and we characterized the autofluorescence (AF) from the various cellular and sub-cellular components of the ENS. (frontiersin.org)
Combined fast clearing and two-photon volume autofluorescence imaging allow for label-free anatomical studies of intestinal tissue and biopsies at unprecedented speed and detail. (frontiersin.org)
As a general category, biomarkers comprise peripheral, tissue biopsy, and imaging methods and allow for a more refined clinical development path forward for therapeutic compounds ( 1 , 2 ). (snmjournals.org)
Cancer cells can occur anywhere in the body, so Yang's imaging method must be detectable through thick tissue mass. (the-scientist.com)
His research group pioneered the development of the first ultra-compact, fully integrated, single-fiber scanning endomicroscope for performing label-free functional imaging of tissue histology in vivo, in situ, and in real time. (hopkinsmedicine.org)
Toghi Eshghi S, Yang S, Wang XC, Shah P, Li XD , Zhang H. "Imaging of N-linked Glycans from Formalin-fixed Paraffin-embedded Tissue Sections Using MALDI Mass Spectrometry," ACS Chemical Biology 9(9):2149-56(2014). (hopkinsmedicine.org)
In this sense, this paper proposes a novel structured optical fibre made of agarose, a transparent, edible material used in culture media and tissue engineering. (nature.com)
Advances in tissue preparation and imaging technologies have allowed researchers to collect vast amounts of targeted structural data, potentially enabling new multi-resolution, multi-modal studies of neural volumes. (springer.com)
The thermal conductivity of water at 310 K is 0.623 watts/m-K and the energy per 500-nm photon is 400 zJ, so for 1 micron 3 of watery tissue the maximum scan rate is ~35 micron 3 /sec-K, if e SNR photons are used to image each 1 nm 3 voxel with SNR = 2. (nanomedicine.com)
The Development of an Innovative Embedded Sensor for the Optical Measurement of an Ex-Vivo Engineered Muscle Tissue Contractility. (azooptics.com)
Noninvasive technologies and methods, such as optical approaches, have recently been developed for measuring tissue contractile force to overcome the shortcomings of traditional systems. (azooptics.com)
In this study , researchers created an embedded sensor that uses an optical tracking algorithm to continuously measure the contractility of synthetic muscle tissue. (azooptics.com)
Confocal Microscope Platform at MultiParkLeica SP8 confocal microscope for imaging of fixed tissue and cells. (lu.se)
Biophotonics is the study of the interaction of light and tissue as well as the development and validation of new optical and imaging diagnostic techniques. (lu.se)
in vivo monitoring of human tissue, as well as industrial applications such as food quality control and spectroscopy of pharmaceutical materials. (lu.se)
Patch clamp recording in vitro (cell cultures) and, to some extent, ex vivo (tissue preparations) to study intrinsic electrical properties of single cells, such as resting membrane potential, membrane resistance, action potential parameters, presence and function of various channels and receptors as well as synaptic connectivity, and to identify, characterize and quantify synaptic transmission. (lu.se)
Extracellular field potential recordings, spontaneous or evoked, to study general network activity, or synaptic plasticity ex vivo in brain tissue preparations (predominantly in hippocampus - LTP, LTD). (lu.se)
Optical imaging of live cells in cultures and tissue preparations to monitor intracellular Ca 2+ fluctuations and to identify population of interest. (lu.se)
We have used different optical techniques to study tissue oxygenation in healthy volunteers. (lu.se)
In-vivo monitoring of tissue oxygen saturation using diffuse reflectance spectroscopy (DRS). Figure: Reistad 2016. (lu.se)
In-vivo monitoring of tissue oxygen saturation and distribution using photoacoustic imaging (PAI). (lu.se)

Noninvasive in vivo imaging technologies play a crucial role in understanding the underlying physiopathology of human diseases through real-time acquisition, processing and quantification of whole-body images. (pharmaceutical-business-review.com)
Functionalized silica-based nanocarriers (SiNPs) can be labeled for in vivo imaging applications and loaded with chemotherapy drugs, making possible the simultaneous noninvasive diagnosis and treatment (theranostic) for HER2-positive BC. (dovepress.com)
Bioluminescence imaging enabled noninvasive, real-time and longitudinal monitoring of the growth of prostate tumors in this model. (helsinki.fi)
The objective of molecular imaging is to characterize, visualize, and quantify normal and pathological biological activities in living organisms at sub-cellular and cellular levels using noninvasive methods. (grandviewresearch.com)

In this study, we evaluated the capability of a Light-Emitting Diode (LED)-based PA and ultrasound (US) imaging system for monitoring heterogeneous microvasculature in tumors (up to 10 mm in depth) and quantitatively compared the PA images with gold standard histology images. (mdpi.com)
For the study, published today in Light: Science & Applications , the team of surgeons, engineers, physicists and material chemists designed and built the optical ultrasound technology to fit into existing single-use medical devices, such as a needle. (sciencedaily.com)
The team developed the all-optical ultrasound imaging technology for use in a clinical setting over four years. (sciencedaily.com)
This is the first demonstration of all-optical ultrasound imaging in a clinically realistic environment. (sciencedaily.com)
A second innovation was the development of highly sensitive optical fibre sensors based on polymer optical microresonators for detecting the ultrasound waves. (sciencedaily.com)
For instance, in April 2022, PerkinElmer, Inc., a US-based company engaged in business areas such as diagnostics, life science research, and imaging, launched the Vega imaging system, an automated advanced technology ultrasound platform. (thebusinessresearchcompany.com)
On the basis of technology, the small animal imaging (in-vivo) market is segmented into micro-MRI, optical, nuclear imaging, and others, including micro-CT and micro-ultrasound. (grandviewresearch.com)
Preoperative diagnosis requires a multimodal diagnostic approach that includes imaging, cystic fluid biochemical analysis, and/or endoscopic ultrasound fine-needle aspiration (EUS-FNA). (medscape.com)

A continuous monitoring of the whole tumor burden of individuals in orthotopic tumor models is a desirable aim and requires non-invasive imaging methods. (nih.gov)
Photoacoustic (PA) imaging has shown tremendous promise for imaging tumor vasculature and its function at deeper penetration depths without the use of exogenous contrast agents. (mdpi.com)
SiNPs-TZ (1:8 Hc-TZ) tumor uptake was significantly greater than that of SiNPs-TZ (1:2 Hc-TZ) at 6 hours post-injection (p.i.) in ex vivo biodistribution experiment. (dovepress.com)
Imaging of 18 F-FDG uptake was performed at 5 time points after drug treatment in the subcutaneously implanted diffuse large B-cell lymphoma tumor line OCI-Ly10. (snmjournals.org)
NIR QDs injected into the tail vein of a mouse can detect tumor metastatic lesions in the lung (red) using the Kodak In Vivo FX Imaging system. (the-scientist.com)
Further, the relative strengths for Optical, microCT, and MRI imaging in tumor progression studies will be reviewed. (bruker.com)
Growth of the orthotopic tumor is difficult to monitor without measuring serum prostate specific antigen (PSA) concentrations or using specific imaging methods. (helsinki.fi)
Bioluminescence imaging is based on a reaction catalyzed by a luciferase which is expressed by the tumor cells. (helsinki.fi)
Bioluminescence imaging was found to correlate better with serum PSA concentrations than with the actual size of the tumor. (helsinki.fi)
However prostate tumor size was noted to correlate better with PSA concentrations than with bioluminescence imaging in this study. (helsinki.fi)
Deep Radiogenomics of Lower-Grade Gliomas: Convolutional Neural Networks Predict Tumor Genomic Subtypes Using MR Images. (cdc.gov)

Hence we devised a new biocompatible hybrid fluorophore composed of near-infrared-emitting PbSe quantum dots encapsulated in solid fatty ester nanoparticles (QD-FEN) for in vivo imaging. (nih.gov)
Image-guided therapy using nanoparticles have been used for various therapeutic applications such as drug and gene delivery, hyperthermia, and photodynamic therapy. (springer.com)
Superparamagnetic iron oxide nanoparticles with variable size and an iron oxidation state as prospective imaging agents. (springer.com)
In vivo delivery, pharmacokinetics, biodistribution and toxicity of iron oxide nanoparticles. (springer.com)
Secondly, nanoparticles were loaded with DOX and their in vitro and ex vivo/in vivo delivery was assessed, in comparison with liposomal Doxorubicin (Caelyx). (dovepress.com)
SiNPs-TZ (1:8 Hc-TZ) nanoparticles loaded with Doxorubicin (DOX-SiNPs-TZ) showed a similar DOX delivery capability than Caelyx (at 6 h p.i.), in in vitro and ex vivo assays. (dovepress.com)
Therapeutic drug or gene is incorporated into nanoparticles to construct multifunctional imaging agents which allow for theranostic applications. (hindawi.com)

Whereas most of the synaptic connections between these neurons have been identified by electron microscopy serial reconstructions, functional connections have been inferred between only a few neurons through combinations of electrophysiology, cell ablation, in vivo calcium imaging and genetic analysis. (nature.com)
To map functional connections between neurons, we combined in vivo optical stimulation with simultaneous calcium imaging. (nature.com)
We present a small, lightweight two-photon fiberscope and demonstrate its suitability for functional imaging in the intact brain. (uzh.ch)
We demonstrate functional imaging of calcium signals in Purkinje cell dendrites in the cerebellum of anesthetized rats. (uzh.ch)
The microscope will be easily portable by a rat or mouse and thus should enable functional imaging in freely behaving animals. (uzh.ch)
Fast, low-angle shoot functional magnetic resonance imaging (fMRI), based on the blood oxygenation level-dependent (BOLD) effect, was combined with optical recording of intrinsic signals (ORIS) and 2-deoxyglucose labeling in gerbil barrel cortex. (jneurosci.org)
Our research studies leverage the strengths of the zebrafish model system by using genetics and in vivo imaging to dissect the molecular and functional requirements underlying hair-cell synapse function and assembly. (amherst.edu)
The advantage of these multimodal instruments is derived from combining the functional strength of one technology, such as SPECT and PET, with the high-resolution anatomical imaging offered by CT or MRI. (grandviewresearch.com)
Here I independently set up a new electrophysiological platform to analyse functional integration of in vivo reprogrammed cells and stem-cell derived neurons. (lu.se)

Figure 1: Challenges facing an all-optical interrogation of neural circuits. (nature.com)
Here, we report a transparent, flexible neural electrode technology based on graphene, which enables simultaneous optical imaging and electrophysiological recording. (nature.com)
Metal microelectrode arrays commonly used for recording neural activity cannot be used for such purposes, as they block the field of view, generate optical shadows and are prone to producing light-induced artefacts in the recordings. (nature.com)
As of yet, neural recordings with completely transparent, flexible electrode arrays, compatible with in vitro and in vivo imaging techniques, have not been demonstrated, to our knowledge. (nature.com)
Unlike learning methods, our Flexible Learning-free Reconstruction of Imaged Neural volumes (FLoRIN) pipeline exploits structure-specific contextual clues and requires no training. (springer.com)
Regional 18F-fluoromisonidazole PET images generated from multiple advanced MR images using neural networks in glioblastoma. (cdc.gov)

We demonstrate that hippocampal slices can be imaged through transparent graphene electrodes by both confocal and two-photon microscopy without causing any light-induced artefacts in the electrical recordings. (nature.com)
Although microscopy techniques used for calcium imaging have generally a small field of view, complete transparency over larger areas is critical to use them with wide-field voltage imaging or in serial calcium imaging experiments to correlate small-scale and large-scale dynamic networks. (nature.com)
or on slicing, often combined with hematoxylin and eosin (H and E) staining and followed by imaging of selected single thin sections with bright-field microscopy. (frontiersin.org)
Brain imaging techniques from microscopy such as serially-sectioned scanning electron microscopy (sSEM), high-energy synchrotron X-ray microtomography ( μ CT), and spectral confocal reflectance (SCoRe) microscopy provide high-quality images with nanoscale or single-neuron resolution. (springer.com)
A new super-resolution method, entitled Near-field Projection Optical Microscopy (NPOM), is presented. (bvsalud.org)
At our microscopy platform in Lund, you meet personnel with expertise and long experience working with biological material and microscopy, imaging, and analysis. (lu.se)
Facilities for 3D optical imaging and electron microscopy at Materials Engineering with instruments including E-SEM FEI Quanta 200 MKII with analytical system Pegasus (EBSD+EDS) from EDAX and optical microscope Keyence VHX-6000. (lu.se)

Chronis, N., Zimmer, M. & Bargmann, C.I. Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans . (nature.com)
A second problem with the optical approach is that individual neuronal response properties are not accessible directly. (jneurosci.org)
To enable in-vivo validation, we introduced BoNT/C-Hc for binding to neuronal plasma membrane and Alexa Fluor 488 for ratiometric measurement. (elifesciences.org)
Different from μ CT X-ray and SCoRe, sSEM imaging provides a more comprehensive view of all neuronal structures within a given volume, allowing one to resolve fine-grained details such as dendritic spines and individual synapses. (springer.com)
Some neuronal circuit tracing studies are based on exhaustive manual proof-reading of results from an algorithm 10 , 11 , which, like the creation of ground-truth data, is not practical for datasets consisting of high resolution images or large volumes. (springer.com)

These image-guided approaches help to achieve more efficient treatments by visualizing biodistribution of therapeutic agents and quantifying them at targeted tissues. (springer.com)
With multi-modality imaging, multiple experimental readouts (anatomy, biodistribution, efficacy, safety and kinetics) are available within the frame of the same study, in the same anatomical context. (pharmaceutical-business-review.com)
Firstly, FITC-filled SiNPs, were engineered with two different amounts of Hc-TZ (trastuzumab half-chain) per single nanoparticle (1:2 and 1:8, SiNPs to Hc-TZ ratio), which was 99m Tc-radiolabeled at histidine residues for ex vivo and in vivo biodistribution evaluations. (dovepress.com)

Dr. Peng will be joined on the webinar by co-presenter Dr. Andrew Van Praagh, Field Applications Scientist, from Bruker BioSpin, who will describe some of the research facilitated by bringing together the products and reagents offered by Imanis with the state-of-the-art multimodal imaging technology delivered by Bruker. (bruker.com)
She will highlight the benefits of multimodal imaging, including early detection, high sensitivity and accelerated drug development. (bruker.com)
Dr. Andrew Van Praagh has a PhD from the University of Massachusetts, Amherst and has been working in the field of multimodal optical imaging for over seven years. (bruker.com)
Radiomics signature for temporal evolution and recurrence patterns of glioblastoma using multimodal magnetic resonance imaging. (cdc.gov)

We investigated the use of a new MMP activatable probe MMPSense ™ 750 FAST (MMPSense750) for in-vivo visualization of early MMP activity in ischemic stroke. (biomedcentral.com)

Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. (lu.se)
Three-dimensional reconstruction of Raman confocal optical images determined that both ND-MWCNT and P-MWCNT were taken up by the cell and localized in the nucleus. (cdc.gov)

The PET and CLI data presented confirm the correlation and dynamic sensitivity of this new imaging modality. (snmjournals.org)
PET is the molecular imaging modality most extensively used in current clinic routine. (hindawi.com)

Radionuclide molecular imaging including PET and SPECT is the earliest and most mature molecular imaging technique. (hindawi.com)

However, in some cases final necropsy revealed the existence of additional, deeper located tumors that had not been detected in vivo by their mPlum signal. (nih.gov)
Consistently, only the weights of the tumors that were detected in vivo based on their mPlum signal correlated with FI. (nih.gov)
Finally, the treatment efficacy of DOX-SiNPs-TZ (1:8 Hc-TZ) was evaluated in vivo by PET and supported by MS-based proteomics profiling of tumors. (dovepress.com)
24 ), who observed a strong correlation between PET and CLI for the imaging of a radiolabeled antibody, 89 Zr-DFO-J591, in LNCaP tumors. (snmjournals.org)
It was also possible to monitor the growth of the tumors more often with bioluminescence imaging than with PSA measurements. (helsinki.fi)
Medical treatment or castration was found to have no effect on the size of the tumors when measured with bioluminescence imaging. (helsinki.fi)
Bioluminescence imaging is not suitable for large or necrotic tumors because this imaging method can only be applied in living cells. (helsinki.fi)
Because of significant overlap in the imaging findings of mucinous and serous pancreatic tumors, these tumors should be followed up with surveillance CT scanning to assess interval growth if aspiration is not performed. (medscape.com)

In vivo visualization of MMP activity would provide important information regarding the spatial and temporal expression of MMP enzymatic activity with respect to the pathophysiology of the disease and to monitor response to interventions, such as therapeutic hypothermia. (biomedcentral.com)
The objective of the present study was to determine whether an alternate MMP activatable probe with a shorter optical imaging time, MMPSense ™ 750 FAST (MMPSense750), could be used for visualization of MMP activity in the early stages of ischemia reperfusion in a mouse model of stroke. (biomedcentral.com)

For instance, Aspect Imaging launched M2 3D MR-based histology system for in-vivo and ex-vivo toxicological imaging. (grandviewresearch.com)
Diffusion Histology Imaging Combining Diffusion Basis Spectrum Imaging (DBSI) and Machine Learning Improves Detection and Classification of Glioblastoma Pathology. (cdc.gov)

The work presented here is an intermediate step in our research towards in vivo endoscopic laryngeal cancer screening. (kent.ac.uk)

Small animal imaging (in-vivo) refers to the advanced imaging techniques for monitoring cellular activity, pharmacological effects that have been developed, and disease progression in live animals. (thebusinessresearchcompany.com)
Molecular imaging is an emerging field that integrates advanced imaging technology with cellular and molecular biology. (hindawi.com)
2/l, and conventional optical imaging will be difficult at the cellular level in vivo ( Section 4.9.4 ). (nanomedicine.com)

Molecular imaging requires high resolution and high sensitive instruments and specific imaging agents that link the imaging signal with molecular event. (hindawi.com)
In this review, we will discuss the characteristics of molecular imaging, the novel imaging agent including targeted imaging agent and multifunctional imaging agent, as well as cite some examples of their application in molecular imaging and therapy. (hindawi.com)
Molecular imaging is a rapidly developed multidiscipline which involves molecular biology, chemistry, computer, engineering, and medicine [ 1 ]. (hindawi.com)
Therefore, molecular imaging plays an important role in earlier detection, accurate diagnosis, and drug development and discovery [ 5 - 7 ]. (hindawi.com)
Therefore, to restore hearing loss after synapse damage is it critical to understand 1) the molecular requirements and 2) the function and assembly of synapses in vivo. (amherst.edu)
Optical redox imaging of ex vivo hippocampal slices differentiates normal from Alzheimer's disease in mouse models World Molecular Imaging Congress, Miami Beach, FL 2022 Notes: Oral presentation. (upenn.edu)
The World Molecular Imaging Congress, Miami Beach, FL 2022 Notes: Oral presentation. (upenn.edu)
It is projected that the growing use of molecular imaging in medicine and biology would drive the development of novel instruments as well as refinement of current technologies. (grandviewresearch.com)

This paper presents an approach for achieving full optical photoacoustic imaging with enhanced resolution utilizing speckle pattern analysis. (bvsalud.org)

Comparisons of responses from adjacent electrodes at successive locations enabled electrophysiological imaging of the two-dimensional pattern of preferred directions across the cortex. (jneurosci.org)
In addition, our results provide electrophysiological confirmation of map features that have been documented in other cortical areas and primate species by optical imaging. (jneurosci.org)
These limitations motivated us to develop an electrophysiological approach that is complementary to optical imaging. (jneurosci.org)

Journal of the Optical Society of America. (uh.edu)

The global small animal imaging (in-vivo) market size grew from $2.28 billion in 2022 to $2.54 billion in 2023 at a compound annual growth rate (CAGR) of 11.4% . (thebusinessresearchcompany.com)
Boris Louis, Multi-dimensional imaging: A tool to unravel material properties at the nanoscale , PhD thesis, Faculty of Science, Lund University 2022. (lu.se)

QDs that excite and emit in the ultraviolet and visible regions have limited in vivo applicability due to significant optical interference exerted by biological fluids and tissues. (nih.gov)
We now have real-time imaging that allows us to differentiate between tissues at a remarkable depth, helping to guide the highest risk moments of these procedures. (sciencedaily.com)
It provides doctors with a live image with a resolution of 64 microns, which is the equivalent of only nine red blood cells, and its fantastic sensitivity allows us to readily differentiate soft tissues," said study co-author, Dr Richard Colchester (UCL Medical Physics & Biomedical Engineering). (sciencedaily.com)
The small animal imaging (in-vivo) is used to observe, define, and quantify normal and pathologic processes in living animal organs and tissues non-invasively. (thebusinessresearchcompany.com)
Micro-MRI refers to a non-invasive technique that produces detailed 3-dimensional (3D) images of tissues without the use of harmful radiation. (thebusinessresearchcompany.com)

Deep-red FPs are more suitable for in vivo applications as compared to eGFP and dsRed2. (nih.gov)
The results confirm that this new sensor is suitable for imaging how ATP signals in the brain, and it may help resolve the underlying mechanisms of migraines and strokes. (elifesciences.org)

The objective of the experimental part of this thesis was to optimize bioluminescence imaging method in androgen responsive cell line LNCaP-luc2 in orthotopic model of prostate cancer. (helsinki.fi)
In addition, a successful luciferin injection is essential for the proper utilization of bioluminescence imaging in this model. (helsinki.fi)

The main components of small animal imaging (in-vivo) are micro-MRI, optical imaging, nuclear imaging, and other components. (thebusinessresearchcompany.com)
Cerenkov luminescence imaging (CLI) is an emerging imaging technique that combines aspects of both optical and nuclear imaging fields. (snmjournals.org)
In this webinar, Dr. Kah-Wye Peng, Chief Operating Officer at Imanis Life Sciences, will outline the range of products and genetic reporters systems and reagents offered by the company for use in optical and nuclear imaging. (bruker.com)

We compared the efficacious effects of MLN4924 using PET and Cerenkov luminescence image values in the same animals. (snmjournals.org)
In previously published work, we demonstrated the first in vivo use of Cerenkov luminescence imaging (CLI) with a highly sensitive and ultracooled charged-coupled device camera, allowing detection of this optical emission ( 15 ). (snmjournals.org)

Zonal ex vivo fluorescent signals were surrounding the ischemic core, and double-positive cells with Cy5.5/exogenous A5 antibody were found in this area. (elsevierpure.com)
One obvious problem is that optical signals cannot be recorded from cortical regions that are buried within sulci. (jneurosci.org)
Here, we propose the fast, label-free 3-D imaging of the ENS, based on intrinsic signals. (frontiersin.org)

Then, we demonstrate the rapid acquisition of detailed 3-D image stacks from unlabeled mouse ileum and colon, across the whole intestinal wall and including both the myenteric and submucosal enteric nervous plexuses using a new spinning-disk two-photon (2P) microscope. (frontiersin.org)
Here, we demonstrate two-photon autofluorescence imaging of NAD(P)H and FAD to nondestructively resolve spatiotemporal metabolic heterogeneity of individual macrophages within 3D microscale TME models. (aacrjournals.org)
Our results demonstrate the feasibility of this optical approach to achieve imaging with enhanced resolution without the need for physical contact with the target, opening up new possibilities for non-invasive medical imaging and other applications. (bvsalud.org)

Zhu D, Liu F, Ma L, Liu D, Wang Z. Nanoparticle-based systems for T1-weighted magnetic resonance imaging contrast agents. (springer.com)
Magnetic resonance imaging (MRI) can be a useful problem-solving adjunct in select cases. (medscape.com)

Other researchers have corroborated and expanded on the current radionuclide list that has been imaged by CLI ( 20 - 23 ). (snmjournals.org)

By processing stacked tubes in a drawing tower, cylindrical biodegradable waveguides can be fabricated with solid or hollow cores, which is desirable for potential applications in chemical measurements and microfluidics, whereas the optical properties and sensing capabilities can be easily tailored by adjusting the fibre geometry 5 , 12 . (nature.com)

B) Optical microscope examination of 1 larva detected from a superficial skin scraping. (cdc.gov)
Near-infrared (NIR) fluorescent images of MMPSense activation were acquired using an Olympus fluorescent microscope, 1.25x objective, a CCD camera and an appropriate filter cube for detecting the activated probe with peak excitation and emission at 749 and 775 nm, respectively. (biomedcentral.com)
A new microscope can generate three-dimensional images of living specimens with a greater combination of depth and precision than before, according to a report in the August 13 Science . (the-scientist.com)
The original Near-field Scanning Optical Microscope (NSOM) surpassed the classical diffraction limit by operating an optical probe at close proximity to the object. (nanomedicine.com)
This novel technique enables the imaging of nanoscale objects without the need for surface scanning, as is usually required in existing methods such as NSOM (near-field scanning optical microscope). (bvsalud.org)
The microscope combines high-resolution optical imaging with depth selectivity which allows us to do optical sectioning. (lu.se)

In vivo imaging of annexin V (A5) was performed at 12 hr, 24 hr, 48 hr, and 4 days after 90-min transient middle cerebral artery occlusion (tMCAO) in mice with a fluorescent protein Cy5.5. (elsevierpure.com)

Simultaneous electrophysiology and optical imaging could leverage the temporal and spatial resolution advantages of both techniques. (nature.com)
Completely transparent microelectrodes can offer a solution for this spatial-temporal resolution dilemma by enabling simultaneous imaging and electrophysiology from the same microcircuit. (nature.com)

1 nm resolution near-field optical scans of in situ protein molecules, since with atomically precise fabrication and single lines of atoms as conductors a minimum light guide (metal-dielectric-metal) is 3 atoms wide. (nanomedicine.com)

Fully automated analysis combining [F]-FET-PET and multiparametric MRI including DSC perfusion and APTw imaging: a promising tool for objective evaluation of glioma progression. (cdc.gov)

Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans . (nature.com)
Calcium imaging is a versatile experimental approach capable of resolving single neurons with single-cell spatial resolution in the brain. (nature.com)
Graphene electrodes record high-frequency bursting activity and slow synaptic potentials that are hard to resolve by multicellular calcium imaging. (nature.com)
Calcium phosphate, a bioresorbable glass, was used to fabricate optical fibres with relatively low attenuation and single-mode characteristics at the near-infrared 2 . (nature.com)

The combination of fast clearing (less than 15 min for 73% transparency), AF detection and rapid volume imaging [less than 1 min for the acquisition of a z-stack of 100 planes (150*150 μm) at sub-300-nm spatial resolution] opens up the possibility for new applications in fundamental and clinical research. (frontiersin.org)
The co-registration between different imaging platforms becomes even more important if small retinal changes are monitored for early detection and treatment. (spie.org)
While these are good techniques they fall short when reporting accurate levels of the active form of MMP, are limited in their sensitivities of MMP detection, and may not be a precise depiction of MMP activity in vivo . (biomedcentral.com)

Zhou Z, Lu Z-R. Gadolinium-based contrast agents for MR cancer imaging. (springer.com)
In contrast to the conventional ex vivo techniques (i.e. immunohistological techniques), in vivo imaging techniques are non-invasive and can therefore be used to perform longitudinal and dynamic studies. (pharmaceutical-business-review.com)

Figure 3: Optical setup for ChR2 stimulation and simultaneous G-CaMP imaging. (nature.com)
Figure 4: Validation of specific stimulation in vivo . (nature.com)

Nanotechnology in medical imaging: probe design and applications. (springer.com)
Biocompatible and resorbable optical fibres emerge as promising technologies for in vivo applications like imaging, light delivery for phototherapy and optogenetics, and localised drug-delivery, as well as for biochemical sensing, wherein the probe can be implanted and then completely absorbed by the organism. (nature.com)
Therefore, the proposed agarose-based structured optical fibre is an easy-to-fabricate, versatile technology with possible applications for medical imaging and in vivo biochemical sensing. (nature.com)
Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo . (cdc.gov)

2D en face spiral scanning pattern is achieved by adjusting the phase between the pairs of X and Y electrodes drive close to 90 degrees. (kent.ac.uk)

The IVIS Spectrum CT enables longitudinal workflows to characterize disease progression and therapeutic effect in animals throughout the complete experimental time frame with both quantitative CT and optical reconstructions. (lu.se)

Optical imaging of individual dye molecules has already been demonstrated, 3195 , 3196 with the ability to determine the orientation and depth of each target molecule located within ~30 nm of the scanned surface. (nanomedicine.com)
493 Molecules with nanometer-scale packing densities have been resolved to ~0.4-nm diameters using STMs to create photon emission maps 494 (an electric current generates photons in the sample), and laser interferometric NSOMs have produced clear optical images of dispersed oil drops on mica to ~1 nm resolution. (nanomedicine.com)

Multidisciplinary expertise and several years of practice are required to develop effective capabilities in non-invasive biomedical 3D imaging exploratory research. (pharmaceutical-business-review.com)
Among the imaging technologies, PET with the glucose analog 18 F-FDG is perhaps the most widely used exploratory imaging biomarker in oncology research and clinical trials ( 7 , 8 ). (snmjournals.org)

The M2 provides a wide range of benefits of MRI including high-resolution 3D images of internal, and anatomical morphology, and quantitative information regarding disease progression and regression. (grandviewresearch.com)

Hypothermia attenuated both the optical increase in intensity after MMPSense750 and the increase in MMP-9 protein expression supporting the proof of concept that NIRF imaging using MMPSense can be used to assess potential therapeutic strategies for stroke treatment. (biomedcentral.com)

Nitrogen-doped MWCNT (ND-MWCNT) has been shown to be less inflammatory in vivo than pristine MWCNT (P-MWCNT) of the same dimensions. (cdc.gov)
Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations. (cdc.gov)

Growing expenditure for the healthcare sector is expected to propel the growth of the small animal imaging (in-vivo) market going forward. (thebusinessresearchcompany.com)

In R&D, imaging is particularly useful for pathophysiology, drug efficacy evaluation and model validation studies. (pharmaceutical-business-review.com)

This system was used to image microscopic retinal structures of healthy as well as diseased retinas in vivo. (spie.org)
The NightShade LB 985 In Vivo Plant Imaging System is a modular, easy to use optical imaging system dedicated to in vivo analysis of plants. (berthold.com)
J.L. Wang, S.M. Zhu, Y.Y. Lin, S. Svanberg, and G.Y. Zhao, Mosquito counting system based on optical sensing, Applied Physics B 126 doi.org/10.1007/s00340-019-7361-2 (2020). (lu.se)

Improvement in instruments and iterative image reconstruction has resulted in high resolution images that reveal tiny lesion and realize accurate quantification of biological process. (hindawi.com)

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes4

Anthropology, Education, Sociology and Social Phenomena1

Information Science1

Health Care1

Fluorescence18

Tomography17

Modalities8

Preclinical16

Tissue33

Noninvasive4

Ultrasound8

Tumor11

Nanoparticles7

Functional9

Neural6

Microscopy7

Neuronal5

Biodistribution3

Multimodal4

Ischemic stroke1

Confocal2

Modality2

SPECT1

Tumors8

Visualization2

Histology2

Endoscopic1

Cellular3

Molecular8

Photoacoustic1

Electrophysiological3

Society of America1

20222

Tissues5

Suitable2

Bioluminescence2

Nuclear3

Luminescence2

Signals3

Demonstrate3

Magnetic2

Radionuclide1

Microfluidics1

Microscope6

Fluorescent1

Electrophysiology2

Situ1

Objective1

Calcium4

Detection3

Contrast2

Stimulation2

Applications4

Electrodes1

Experimental1

Molecules2

Exploratory2

Morphology1

Therapeutic1

Inflammatory2

Growth1

Validation1

System3

Quantification1