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Hematopoietic Stem Cell TransplantationHematopoietic Stem CellsStem CellsStem Cell NicheGraft vs Host DiseaseStem Cell TransplantationTransplantation, HomologousHematologic NeoplasmsTransplantation ConditioningTransplantation, AutologousCell DifferentiationHematopoiesisAdult Stem CellsTransplantationPancytopeniaHistocompatibility TestingHepatic Veno-Occlusive DiseaseCell LineageMice, Inbred C57BLImmunocompromised HostEmbryonic Stem CellsAntigens, CD34Peripheral Blood Stem Cell TransplantationColony-Forming Units AssayBone Marrow CellsBone MarrowLeukemiaBone Marrow TransplantationTransplantation ChimeraFlow CytometryTissue DonorsPluripotent Stem CellsFetal BloodCell ProliferationCells, CulturedAnemia, AplasticStromal CellsImmunosuppressive AgentsTreatment OutcomeHLA AntigensCell CountNeural Stem CellsInduced Pluripotent Stem CellsMultipotent Stem CellsMesenchymal Stem Cell TransplantationSignal TransductionAntifungal AgentsStem Cell FactorMesenchymal Stromal CellsGene Expression RegulationRetrospective StudiesFollow-Up StudiesStem Cell ResearchPlant StemsFetal Stem CellsCell SurvivalHematopoietic Stem Cell MobilizationCell DivisionNeoplastic Stem CellsRegenerationOctamer Transcription Factor-3Cell Culture TechniquesRegenerative MedicineCell SeparationTotipotent Stem CellsEmbryo, MammalianSOXB1 Transcription FactorsSpermatogoniaCell- and Tissue-Based TherapyMice, SCIDTissue EngineeringGene Expression Regulation, DevelopmentalProto-Oncogene Proteins c-kitCord Blood Stem Cell TransplantationNestinCell LineReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingGerm CellsHomeodomain ProteinsHair FollicleBrain StemEmbryo ResearchPhenotypeBiological MarkersCoculture TechniquesMice, TransgenicNeuronsCell DedifferentiationModels, BiologicalImmunohistochemistryTranscription FactorsGreen Fluorescent ProteinsTeratomaAntigens, CDEmbryoid BodiesNuclear ReprogrammingSpheroids, CellularCell TransplantationNeurogenesis
TransplantationProgenitorHSCsDifferentiationProgenitorsFlow cytometryHematopoiesisMiceLineageLymphocytesAssaysHSPCsBiologyGrowth of hematopoieticEngraftmentLineagesExpansionReconstitutionProteinsFetalPeripheral bloodInducesHSPCMultipotentSelf-renewalHSCTRegulatorMesenchymal stemFound in the bone maCordTherapiesAdultTumorGenesTherapyReceptorVectorsErythroidCD34NicheImmunePrimary immunodeficiencyTherapeuticGene transferTransductionEmbryonic2020Human pluripotent stemGenerateRepopulationInnatePatients
Transplantation37
Progenitor28
  • Scholars@Duke publication: Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo. (duke.edu)
  • BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. (duke.edu)
  • Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
  • Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
  • However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
  • Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • Stem and progenitor cells can be taken from the pelvis, at the iliac crest, using a needle and syringe. (wikipedia.org)
  • It was used extensively in early studies, but is now considered to measure more mature progenitor or transit-amplifying cells rather than stem cells[citation needed]. (wikipedia.org)
  • Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
  • Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
  • Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
  • Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
  • We observed enhanced progenitor cell activity within the CD34+ cell population treated with Delta1 in hypoxia, but the benefits provided by low-oxygen cultures were most notable in the primitive HSC compartment. (confex.com)
  • Most clusters could be computationally assigned to a defined hematopoietic subpopulation, including progenitor cells (clusters 0 to 4) and a single transcriptionally defined HSC population (cluster 5). (confex.com)
  • In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
  • A readily available, inexpensive, small molecule drug can improve the fitness of hematopoietic stem and progenitor cells (HSPCs) that are modified outside the body, potentially improving the success of procedures such as ex vivo gene therapy, according to a new study by researchers at Children's Hospital of Philadelphia (CHOP). (phys.org)
  • To our surprise, we found that in the absence of any manipulation, endothelial cells could direct differentiation and support the expansion of CD34+ cells (progenitor blood cells) to a very specific blood cell type, named macrophages. (ca.gov)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • The allogeneic HSCT was the most frequently performed (57.14%) and the most used source of Hematopoietic progenitor cells (HPC) was the peripheral blood (54.29%) and 5.71% of these patients developed the Graft versus Host Disease (GVHD), of which one was affected by acute GVHD and another by chronic GVHD. (bvsalud.org)
  • [ 18 ] Arylsulfatase A deficiency leads to defective glial and neuronal differentiation from neural progenitor cells. (medscape.com)
  • In a second line, we use natural genetic variation to find genes that regulate hematopoietic stem and progenitor cells (HSPC) in humans. (lu.se)
  • Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
HSCs25
  • In steady-state adult hematopoiesis, most hematopoietic stem cells (HSCs) are in the resting phase of the cell cycle. (uzh.ch)
  • Upon enhanced hematopoietic demand, HSCs can be induced to divide and self-renew or differentiate. (uzh.ch)
  • Using in vivo high-resolution single HSC divisional tracking, we directly demonstrate that clinically applied thrombopoietin receptor but not granulocyte colony-stimulating factor (G-CSF) receptor agonists drive HSCs into self-renewing divisions leading to quantitative expansion of functional HSC as defined by their in vivo serial multilineage and long-term repopulating potential. (uzh.ch)
  • These results suggest that thrombopoietin mimetics might be applicable to expand HSCs in vivo and to sensitize thrombopoietin receptor-expressing HSCs to cell cycle-dependent cytotoxic agents. (uzh.ch)
  • Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. (duke.edu)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
  • Hematopoietic stem cells (HSCs) are the stem cells that give rise to other blood cells. (wikipedia.org)
  • In vertebrates, the very first definitive HSCs arise from the ventral endothelial wall of the embryonic aorta within the (midgestational) aorta-gonad-mesonephros region, through a process known as endothelial-to-hematopoietic transition. (wikipedia.org)
  • Because a larger fraction of LKS in the BM of treated mice was found in the S/M phase of the cell cycle, osteoclast impairment makes a proportion of HSCs enter the cell cycle and differentiate. (ox.ac.uk)
  • Subjecting transduced HSCs to an ex vivo expansion protocol following the transduction procedure could partially reverse this loss. (ox.ac.uk)
  • CONCLUSIONS: These studies suggest that loss of repopulating ability of transduced HSCs rather than low gene transfer efficiency might be the main problem in clinical gene therapy protocols, and that a clinically feasible ex vivo expansion approach post-transduction can markedly improve reconstitution with gene marked stem cells. (ox.ac.uk)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • We found that HSCs from Lnk-/- mice have an increased quiescent fraction, decelerated cell cycle kinetics, and enhanced resistance to repeat treatments with cytoablative 5-fluorouracil in vivo compared with WT HSCs. (jci.org)
  • Lnk -/- LSK HSCs show decelerated cell cycle kinetics. (jci.org)
  • We have identified several new growth factors, and established a very potent cell culture system for growing human HSCs. (gabriellesangels.org)
  • Here, we investigated whether ex vivo culture of HSPCs under hypoxia might limit cellular ER stress and thus offer a simple approach to preserve functional HSCs under high proliferative conditions, such as those promoted in culture with Delta1. (confex.com)
  • To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
  • To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
  • Their approach involves modifying hematopoietic stem cells (HSCs) ex vivo to obtain the expression of therapeutic proteins in the erythroid lineage, i.e., the cell line resulting in red blood cells. (genopole.com)
  • The funding from the Gates Foundation will advance Intellia's preclinical validation of in vivo hematopoietic stem cells (HSCs) genome editing using the company's proprietary non-viral delivery systems and CRISPR/Cas9 technology to potentially cure SCD. (newswise.com)
  • Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
  • However, metabolic processes underlying the emergence of HSCs from HE cells remain unclear. (lu.se)
Differentiation16
  • Accordingly, enforced expression of SKI induced a gene signature associated with hematopoietic stem cells and myeloid differentiation, as well as hepatocyte growth factor signaling. (edu.au)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-foetal globin genes in human erythroleukemia K562 cells, bioRvix. (davidicke.com)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • However, also ex vivo, primary cells are not homogeneous and may well differ, for example, in the differentiation state. (hstalks.com)
  • In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
  • Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
  • And ex vivo activation in prolonged culture has been shown, especially when using imperfect methodology in experiment, to induce differentiation or even it extinguishes the stem cell properties. (hstalks.com)
  • TGF-β affects development and differentiation of human natural killer cell subsets. (sunnybrook.ca)
  • HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation. (sunnybrook.ca)
  • We have found that inflammatory signals, particularly interferon gamma, influence the proliferation and differentiation of hematopoietic stem cells during infection, thus shaping the immune response as a whole. (texaschildrens.org)
  • Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
  • Our findings reveal that during EHT, metabolism is a major regulator of primitive versus definitive hematopoietic differentiation. (lu.se)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Progenitors6
  • Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
  • 02:14 progenitors that make red blood cells. (yale.edu)
  • The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
  • Left panels show representative FACS plots of Lin - Kit hi BM cells gated for LSK and LK HSC progenitors. (jci.org)
  • Rac2 GTPase deficiency depletes BCR-ABL+ leukemic stem cells and progenitors in vivo. (alexslemonade.org)
  • Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors. (sunnybrook.ca)
Flow cytometry5
  • citation needed] Hematopoietic stem cells can be identified or isolated by the use of flow cytometry where the combination of several different cell surface markers (particularly CD34) are used to separate the rare Hematopoietic stem cells from the surrounding blood cells. (wikipedia.org)
  • By using multiparametric flow cytometry , we confirmed the expansion of NK cells expressing CD9 immediately after autoHSCT, which exhibited higher granzyme B and perforin expression levels than CD9- NK cells . (bvsalud.org)
  • Flow cytometry and data analysis was performed using a BD LSRFortessa™ Cell Analyzer System X-20 and FlowJo™ software. (bdbiosciences.com)
  • In addition, my lab studies the process of developmental committment to the B cell lineages, using 7-color flow cytometry on a state-of-the-art 3 laser FACS. (umassmed.edu)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
Hematopoiesis3
  • These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo. (duke.edu)
  • In vertebrates, the vast majority of hematopoiesis occurs in the bone marrow and is derived from a limited number of hematopoietic stem cells that are multipotent and capable of extensive self-renewal. (wikipedia.org)
  • The team's approach combined the advantages of maximizing the quantity of therapeutic proteins produced and placing that production in the most populous cells resulting from hematopoiesis, that is, those involved in and resulting from erythropoiesis. (genopole.com)
Mice16
  • Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
  • Another CFU, the colony-forming unit-spleen (CFU-S), was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. (wikipedia.org)
  • Mice treated with bisphosphonates exhibited a decrease in proportion and absolute number of Lin(-)cKit(+)Sca1(+) Flk2(-) (LKS Flk2(-)) and long-term culture-initiating cells in bone marrow (BM). (ox.ac.uk)
  • Treated recipient mice experienced a delayed hematopoietic recovery compared with untreated controls. (ox.ac.uk)
  • Despite using optimized culture conditions, transduction resulted in striking losses of repopulating activity, translating into low numbers of gene marked cells in competitively repopulated mice. (ox.ac.uk)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • We further find a reduced T cell abundance in B cell lymphomas from Atm-defective mice and A-T patients. (nih.gov)
  • No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
  • Using HLA-A2.1 transgenic mice, we have demonstrated the feasibility of T-cell receptor (TCR) gene transfer into T cells to circumvent self-tolerance to the widely expressed human p53 and MDM2 TAAs. (unimedizin-mainz.de)
  • Immature human red blood cells in the bone marrow of mice infected with the parasite Plasmodium vivax. (pasteur.fr)
  • By engrafting human hematopoietic stem cells into mice, a team from the Institut Pasteur, Inserm and the CNRS was able to maintain the Plasmodium vivax parasite in vivo, providing a novel model to explore therapeutic strategies. (pasteur.fr)
  • To reconstitute interactions between P. vivax and its human host more effectively, the scientists worked on mice engrafted with human hematopoietic stem cells. (pasteur.fr)
  • The bone marrow of these mice is capable of producing human blood cells, including red blood cells that are targeted by the parasites," explains Sylvie Garcia, a group leader in the Biology of Host-Parasite Interactions Unit . (pasteur.fr)
  • By producing immature red blood cells in the bone marrow at the stage when they are infected by P. vivax , these mice provide an in vivo model in which the parasite can be maintained. (pasteur.fr)
  • This has been shown both in mice transplanted with mouse cells, as well as in mice transplanted with human cells in an American experiment, and very recently in humans transplanted with human cells in clinical trials. (hstalks.com)
  • Yet, most of what we know comes from studies in vitro and in mice, and little is known about the situation in vivo in humans. (lu.se)
Lineage6
  • Lack of expression of lineage markers is used in combination with detection of several positive cell-surface markers to isolate hematopoietic stem cells. (wikipedia.org)
  • GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
  • Enforcement of γδ-lineage commitment by the pre-T-cell receptor in precursors with weak γδ-TCR signals. (sunnybrook.ca)
  • Context-dependent regulation of hematopoietic lineage choice by HEBAlt. (sunnybrook.ca)
  • Hematopoietic stem cell lineage choices are decided by genetic networks that are turned ON/OFF in a switch-like manner. (lu.se)
  • We develop a computational model for the hematopoietic erythroid-myeloid lineage decision, which is determined by a genetic switch involving the genes PU.1 and GATA-1. (lu.se)
Lymphocytes6
  • In shape, hematopoietic stem cells resemble lymphocytes. (wikipedia.org)
  • Lymphoblast cells are immature white blood cells that develop into healthy immune cells called lymphocytes. (davidicke.com)
  • I will then show you a series of examples covering a broad variety of target subtypes, including lymphocytes, hematopoietic stem cells, neurons, and endothelial cells. (hstalks.com)
  • Immunotherapy based on the adoptive transfer of ex vivo expanded and activated autologous tumor-reactive lymphocytes can mediate tumor regression in cancer patients. (unimedizin-mainz.de)
  • A pilot phase I clinical trial to assess the effects of cell therapy using autologous Epstein-Barr virus-specific cytotoxic T-lymphocytes in individuals presenting for the first time signs of multiple sclerosis is underway. (afm-telethon.fr)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
Assays3
  • In this webinar, CFC assay expert Dr. Jackie Damen will describe how customized hematopoietic CFC assays can be applied to assist you in the successful development of your small molecule compounds and biologics. (stemcell.com)
  • This property forms the basis of in vivo assays of HSC function. (stemcell.com)
  • We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)
HSPCs4
  • We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
  • In some cases, as in ex vivo gene therapy, the patient acts as their own donor: the patient's HSPCs are removed, modified outside of the body using gene addition or gene editing, and then transplanted back in. (phys.org)
Biology7
  • Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
  • Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
  • To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
  • In parallel, the development of animal models opened new opportunities to study parasite biology in vivo . (wjgnet.com)
  • This research will lead to critical insights into the basic biology of hematopoietic stem cell function, including how inflammation may contribute to hematologic changes seen in aging and cancer. (texaschildrens.org)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
Growth of hematopoietic1
  • The first step was to test whether human endothelial cells could offer a supportive niche for the growth of hematopoietic cells. (ca.gov)
Engraftment1
Lineages1
  • Myeloid and lymphoid lineages both are involved in dendritic cell formation. (wikipedia.org)
Expansion2
Reconstitution3
  • Major emphasis has so far been directed at improving gene transfer efficiency, but low gene marking in transplanted recipients might equally well reflect compromised repopulating activity of transduced cells, competing for reconstitution with endogenous and unmanipulated stem cells. (ox.ac.uk)
  • To uncover global changes during NK cell reconstitution after autoHSCT, we performed RNA -sequencing on NK cells before and after autoHSCT. (bvsalud.org)
  • These results provide insights into the physiopathology of NK cells during their reconstitution after autoHSCT. (bvsalud.org)
Proteins3
  • b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
  • Ultimately secreted into the blood, those proteins are captured by the pathological cells wherein they are able to correct specific disorders. (genopole.com)
  • Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins. (genopole.com)
Fetal2
  • Developmental progression of fetal HEB(-/-) precursors to the pre-T-cell stage is restored by HEBAlt. (sunnybrook.ca)
  • HEBAlt enhances the T-cell potential of fetal myeloid-biased precursors. (sunnybrook.ca)
Peripheral blood1
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
Induces2
  • Here, we show that Atm reactivation in initially Atm-deficient B- and T cell lymphomas induces tumor regression. (nih.gov)
  • Correction: Nasopharyngeal Exposure to Streptococcus pneumoniae Induces Extended Age-Dependent Protection against Pulmonary Infection Mediated by Antibodies and CD138+ Cells. (umassmed.edu)
HSPC2
  • For this reason, researchers have sought to better understand HSPC programming and signaling so that every attempt could be made to maintain cell health ahead of transplant. (phys.org)
  • This important study, which links EV trafficking and the integrated stress response as a regulatory dyad guarding HSPC integrity and long-term fitness, has important implications for potentially broadening patient eligibility to ex vivo gene therapies. (phys.org)
Multipotent2
  • Hematopoietic stem cells can replenish all blood cell types (i.e., are multipotent) and self-renew. (wikipedia.org)
  • RESULTS: We demonstrate that conditions supporting in vitro stem cell self-renewal efficiently promote oncoretroviral-mediated gene transfer to multipotent adult bone marrow stem cells, without prior in vivo conditioning. (ox.ac.uk)
Self-renewal1
  • Therefore, we identified Lnk as a physiological negative regulator of JAK2 in stem cells and TPO/Mpl/JAK2/Lnk as a major regulatory pathway in controlling stem cell self-renewal and quiescence. (jci.org)
HSCT2
  • Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
  • The overall death infections that occurred in 2 clusters of hematopoietic stem rate was 50%, and death occurred within 8 days after diag- cell transplant (HSCT) recipients in our institution prompt- nostic culture collection. (cdc.gov)
Regulator2
  • Collectively our results demonstrate that SKI is an important regulator of hematopoietic stem cell activity and its overexpression leads to myeloproliferative disease. (edu.au)
  • It may be a major regulator of tumor angiogenesis in vivo. (ihcworld.com)
Mesenchymal stem2
Found in the bone ma2
Cord3
  • DESCRIPTION (provided by applicant): In the past years, we have shown that human umbilical cord blood cells (HUCBC, U-CORD- CELL ) provide cognitive recovery in animal models of neurodegenerative disease. (sbir.gov)
  • One concern about the procedure is that immune cells in cord blood do not have experience with the viruses responsible for latent infections, leading to a higher percentage of naive T cells and thus increased vulnerability to reactivation of cytomegalovirus or Epstein-Barr virus infection. (msdmanuals.com)
  • Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
Therapies4
  • Collectively, these findings indicate that thorough analyses are needed to uncover rare preexisting resistant cells and that unbiased molecular analyses can identify therapies to counter resistance. (aacrjournals.org)
  • The project, selected by the European Innovation Council, aims to resolve the difficulty of T-cell exhaustion in CAR-T therapies for solid tumors. (genopole.com)
  • Intellia's in vivo CRISPR/Cas9 delivery approach has the potential to develop HSC-based genome editing therapies that are more practical solutions to treat blood disorders, including SCD. (newswise.com)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Adult3
  • haematopoietic) is the medical term used to describe the process by which our blood cells are formed, develop and mature into their final "adult" types. (davidicke.com)
  • Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
  • Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)
Tumor6
  • However, high-avidity T cells specific for tumor-associated antigens (TAAs) are usually absent in patients because of self-tolerance. (unimedizin-mainz.de)
  • T cells and TCR will be obtained from blood and tumor tissue and tested for neoantigen specificity and tumor reactivity. (unimedizin-mainz.de)
  • Versican G3 promotes mouse mammary tumor cell growth, migration, and metastasis by influencing EGF receptor signaling. (sunnybrook.ca)
  • In vivo , adding an S6K inhibitor to MEKi plus CDK4i treatment reverted PI3KCA E545K -mediated resistance, inducing durable tumor regression in melanoma xenografts. (aacrjournals.org)
  • Analogous studies in a murine syngeneic tumor model using surrogate antibodies demonstrated significant synergy between LAG-3 and PD-1 blockade-combination treatment led to a marked improvement in therapeutic efficacy, increased T-cell proliferation, IFNγ production, and elicited durable immunologic memory upon tumor rechallenge. (aacrjournals.org)
  • Tumor persistence leads to sustained exposure of T cells to neoantigens-this results in repeated cycles of activation which culminate in a state of severe T-cell dysfunction or exhaustion, characterized by distinct epigenetic and transcriptional profiles ( 1-3 ). (aacrjournals.org)
Genes8
  • RNA interference is a gene therapy that could potentially be used to "treat a variety of diseases" by delivering short strands of RNA that block specific genes from being turned on in a cell. (davidicke.com)
  • Using this gene therapy technique with specialised nanoparticles, researchers from Penn Engineering and the Massachusetts Institute of Technology ("MIT") developed a way to turn off specific genes in cells of bone marrow. (davidicke.com)
  • Intellia believes the CRISPR/Cas9 technology has the potential to transform medicine by both producing therapeutics that permanently edit and/or correct disease-associated genes in the human body with a single treatment course, and creating enhanced engineered cells that can treat oncological and immunological diseases. (newswise.com)
  • In addition, we developed a series of novel tools to test the biological relevance of the genes identified in vivo (using mouse embryos). (ca.gov)
  • Specifically, we have tested whether forced expression of these genes could induce the generation of hematopoietic stem cells. (ca.gov)
  • In addition, the generation of two GFP mutants with distinct excitation optima allows for detection of two distinct GFP genes within single cells. (umassmed.edu)
  • 1 V(D)J recombination and the activity of genes of interest (using GFP as a reporter of gene expression) can be detected as intracellular fluorescence and are measured simultaneously in the same cell population, at the single cell level. (umassmed.edu)
  • However, prior to commitment, it has been cells, it is evident that GATA-1 and PU.1 are able to specify observed that many genes are expressed at intermediate or basal erythroid and myeloid cell fates (see [16] and references therein). (lu.se)
Therapy11
  • BACKGROUND: Extensive efforts to develop hematopoietic stem cell (HSC) based gene therapy have been hampered by low gene marking. (ox.ac.uk)
  • We believe that engineering nanoparticles to deliver RNA to different types of cells and organs in the body is key to reaching the broadest potential of genetic therapy. (davidicke.com)
  • In gene therapy, we do basically distinguish between in vivo and ex vivo modification of target cells. (hstalks.com)
  • It is obvious that upon in vivo applications, vectors will encounter a large variety of cell types besides the actual target cells relevant for therapy. (hstalks.com)
  • And now we start from discussing ex vivo application into hematopoietic cell gene therapy in particular hematopoietic stem cell gene therapy. (hstalks.com)
  • Ultimately, this serendipitous finding could enhance the quality of transplant products used in treatments like ex vivo gene therapy, improving patient outcomes . (phys.org)
  • AFM-Telethon has supported and continues to support the development (clinical trials underway or in preparation) of over forty drugs in gene therapy, cell therapy and pharmacology. (afm-telethon.fr)
  • Strimvelis® (gene therapy of the bone marrow stem cells) for X-linked severe combined immune deficiency. (afm-telethon.fr)
  • Zynteglo® (gene therapy of the bone marrow stem cells) for beta thalassemia. (afm-telethon.fr)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • A recent study by Passerini et al (2013) highlights the possibility of using adoptive cell therapy with genetically engineered regulatory T cells as a novel therapy for IPEX syndrome. (medscape.com)
Receptor3
  • Retroviral vectors are envelope particles that bind with their surface receptor for cell entry. (hstalks.com)
  • OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism. (umassmed.edu)
  • Lymphocyte activation gene-3 (LAG-3) is a coinhibitory receptor associated with impaired T-cell function and is frequently coexpressed with programmed cell death protein-1 (PD-1) in the context of human cancers. (aacrjournals.org)
Vectors6
  • We will first focus on ex vivo applications and then go through some examples of in vivo administrations of the targeted vectors. (hstalks.com)
  • By in vivo, we mean that vectors are directly injected into the patient, which can be locally, such as intracerebrally, or systemically. (hstalks.com)
  • The next slide shows and summarizes the fact that several work from the early days after the developmental of the lenvitiral vectors show that, indeed, this vector had a benefit of improved gene transfer into hematopoietic stem cell. (hstalks.com)
  • And this is mostly due to the fact that because, as mentioned several times, lentiviral vectors do not rely on active proliferation of the site at the time of infection, they don't require as much culture ex vivo and as much proliferation of the cell that they infect as the gamma lentiviral vector do. (hstalks.com)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Erythroid2
  • In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
  • Citation: Chickarmane V, Enver T, Peterson C (2009) Computational Modeling of the Hematopoietic Erythroid-Myeloid Switch Reveals Insights into Cooperativity, Priming, and Irreversibility. (lu.se)
CD342
  • CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
  • EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
Niche4
  • If we could develop technologies that could control cellular activity in bone marrow and the hematopoietic stem cell niche, it could be transformative for disease applications," said Michael Mitchell, one of the study's lead authors. (davidicke.com)
  • These were rather unexpected results that indicated the ability of endothelial cells to offer a niche for a selective group of blood cells. (ca.gov)
  • Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
  • Stem cell niche. (bvsalud.org)
Immune7
  • Mitchell was already working on new nanotechnologies that target bone marrow and immune cells for treating other diseases, especially blood cancers such as multiple myeloma. (davidicke.com)
  • Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
  • However, maintaining the fitness of these cells outside of the body poses a challenge, and any loss of fitness introduces the risk that the cells will not properly restore the blood and immune cell system in patients. (phys.org)
  • The host immune system can be viewed as a product of hematopoietic stem cells that reside in the bone marrow. (texaschildrens.org)
  • My research is focused on how the composition of the immune system is regulated, starting at the level of the hematopoietic stem cell. (texaschildrens.org)
  • This work provides insight into how immune cells are sustained during infection, when the demand for immune effector cells is dramatically increased. (texaschildrens.org)
  • In a humanized mouse non-small cell lung carcinoma model, TSR-033 boosted the antitumor efficacy of PD-1 monotherapy, with a concomitant increase in immune activation. (aacrjournals.org)
Primary immunodeficiency1
  • Regulatory T cells in primary immunodeficiency diseases. (medscape.com)
Therapeutic3
  • Previous work from the contributing DKTK partner sites has demonstrated the versatility of this approach in T cells, and showed that it is feasible to derive CAR-engineered, established human NK cells as an off-the-shelf cellular therapeutic. (unimedizin-mainz.de)
  • DESCRIPTION (provided by applicant): Therapeutic stem cell gene transfer relies on long-term gene expression achieved by integration of new DNA into the cellular genome. (sbir.gov)
  • Molecule screening on human motor neurons produced from pluripotent stem cells helped identify the therapeutic potential of lithium in this genetic form of autism. (afm-telethon.fr)
Gene transfer1
  • A safety concern of TCR gene transfer is the risk of pairing between introduced and the naturally expressed endogenous TCR chains, resulting in the generation of self-reactive T cells. (unimedizin-mainz.de)
Transduction1
  • Upon harvesting an ex vivo transduction, they may need to be activated. (hstalks.com)
Embryonic3
20201
  • Newswise - CAMBRIDGE, Mass., Nov. 11, 2020 - Intellia Therapeutics, Inc. (NASDAQ:NTLA), announced that it has received a grant from the Bill & Melinda Gates Foundation to research in vivo sickle cell disease (SCD) treatments using its CRISPR/Cas9 genome editing technology. (newswise.com)
Human pluripotent stem3
  • We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
Generate3
Repopulation1
  • And so lentiviral vector allows a much more efficient infection, which translate into shorter ex vivo culture, which means when these transducers are transplanted into recipients into a polyclonal, a more efficient repopulation of the host by gene-corrected cell. (hstalks.com)
Innate1
  • Lymphoid cells include T cells, B cells, natural killer cells, and innate lymphoid cells. (wikipedia.org)
Patients6
  • The number and function of proangiogenic cells are impaired in patients with diabetes or cardiovascular diseases. (hindawi.com)
  • These particles could be tailored to help treat heart disease or to boost the yield of stem cells in patients who need stem cell transplants, Penn Engineering Today wrote. (davidicke.com)
  • Fifteen patients have been transplanted using such a regimen of whom 13 underwent ex vivo TCD. (qub.ac.uk)
  • Chimeric antigen receptors (CAR)-engineered T cells have demonstrated remarkable efficacy in patients with B-cell malignancies. (unimedizin-mainz.de)
  • In an interview with Medscape Medical News, Sarin said that although the use of G-CSF for patients with severe SAH is still under investigation at his center, "we are using G-CSF routinely for decompensated cirrhosis, where it is like an in vivo extension of regenerative stem cells. (medscape.com)
  • En outre, il y avait une aug- mentation significative de la forme soluble du c-kit dans le sérum des patients atteints de pemphigus vulgaire actif par rapport aux témoins. (who.int)