Vivo expansion of human hematopoietic stem. Medical search. Frequent questions

In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Single-cell assessment of transcriptome alterations induced by Scriptaid in early differentiated human haematopoietic progenitors during ex vivo expansion. (ox.ac.uk)
We employed in situ hybridization and immunocytochemical staining to monitor gene expression, and 5-bromo-2-deoxyuridine (BrdU), primary liquid cultures, and clonogenic progenitor assays to correlate AChE-R and ARP with proliferation and differentiation of hematopoietic progenitors. (tau.ac.il)

The ability to grow blood stem cells (HSCs) in culture would greatly improve the patient outcome in transplantation, and make cord blood to be useful for adult applications. (gabriellesangels.org)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
In addition, short-term osteoinductive treatment favored in vivo hematopoietic recovery in lethally irradiated mice that underwent BM transplantation. (elsevierpure.com)
Our findings demonstrate the unique biological characteristics of e-MSCs as hematopoiesis-regulatory stromal cells at differentiation stage between MSCs and osteoprogenitor cells and have significant implications in developing new strategy for using pharmacological osteoinductive treatment to support hematopoiesis in hematopoietic stem and progenitor cell transplantation. (elsevierpure.com)
So, efforts for applying cord blood stem cells in transplantation have led to establishment of some approaches for ex vivo expansion of HSCs such as garcinol. (civilica.com)
Hematopoietic stem cell transplantation is a curative therapy for many patients suffering from life threatening blood disorders. (lu.se)
We predicted that precise information related to the operational factors in the embryo would allow us to reproduce this process in a petri dish and generate hematopoietic stem cells when needed (situations associated with blood transplantation or cancer). (ca.gov)
Moreover, HSPCs are of Major relevance for the establishment of successful hematopoietic stem cell transplantation (HSCT) strategies. (stemcell-lab-mk1dresden.de)
2003). After the early success of transplantation of cord blood from related donors, cord blood banks were established to provide rapidly accessible, human leukocyte antigen (HLA)-typed units predominantly for transplantation of HPCs from unrelated donors. (nationalacademies.org)
In vivo transplantation demonstrated that LIFR+PDGFRB+CD45-CD31-CD235a- MSCs could form bone tissues and reconstitute the hematopoietic microenvironment (HME) effectively in vivo. (bjmu.edu.cn)
Risk-adapted therapies for AML, ALL, and CLL patients consist of chemotherapy with/without stem cell transplantation (SCT), but the rate of early failures and relapses is still unsatisfying. (nature.com)
Guiding Principles on Human Organ Transplantation. (who.int)
Member States to take appropriate measures to prevent the purchase and sale of human organs for transplantation (resolution WHA42.5). (who.int)
Although the number of transplantations performed each year has grown rapidly since the Guiding Principles were endorsed, there has also been a significant increase in the demand for transplantation using human cells, tissues and organs, resulting in a continuing shortage of human material, particularly organs. (who.int)
This practice, which includes the provision of human material for transplantation, should not be confused with the purchase abroad of medical care only. (who.int)

The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
The clinical application of MSCs to tissue engineering has been introduced due to their numerous advantages including high expansion potential and extensive differentiation potential [ 6 , 7 ]. (hindawi.com)
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. (stemcell.com)
Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
In a study published in the journal Blood, the Larsson group targeted the enzyme LSD1, part of the CoREST complex, known to mediate the epigenetic modification of DNA and pushing the differentiation of stem cells into mature blood cells. (lu.se)
We hypothesised that by reducing LSD1 levels using small molecule drugs we could halt stem cell differentiation and stimulate expansion. (lu.se)
Ex vivo, ARP was more effective than cortisol and equally as effective as stem cell factor in promoting expansion and differentiation of early hematopoietic progenitor cells into myeloid and megakaryocyte lineages. (tau.ac.il)
To our surprise, we found that in the absence of any manipulation, endothelial cells could direct differentiation and support the expansion of CD34+ cells (progenitor blood cells) to a very specific blood cell type, named macrophages. (ca.gov)
Previous studies have reported changes in the transcriptional program of genes involved in proliferation, differentiation, apoptosis, and homing when HSPCs were expanded ex vivo. (ox.ac.uk)
B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
We found that many bone marrow stromal cells have the ability of multi-lineage differentiation potential and CFU-F activity in vitro, but failed to form new bone when transplanted in vivo. (bjmu.edu.cn)
In this proposal, we seek to evaluate the integration of K562/GM-CSF vaccination with systemic therapies for MDS that alter host immunity and/or hematopoietic cellular differentiation. (acgtfoundation.org)
Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
Objective: To characterize pulp stem cells and evaluate their capacity for expansion and differentiation in vitro. (bvsalud.org)
Results: It was observed that the pulp stem cells exhibited the capacity to adhere to plastic and a high rate of expansion and, after detection with specific stains, it was shown that the cells were capable of differentiation into osteoblasts and chondroblasts, but not into adipocytes. (bvsalud.org)
Stem cells are cells with a low degree of differentiation that have the capacity to reproduce and can generate differentiated cells of several different types of tissues 1 . (bvsalud.org)
The objective of this study is to isolate and characterize stem cells from the pulp of permanent third molars and analyze the cells obtained in terms of their morphology and chondrogenic, osteogenic and adipogenic cell differentiation. (bvsalud.org)

There was no significant difference in the transcriptomic profile, by RNAsequencing, between cytokine-expanded and purified Lin-CD34+CD38-CD45RA-CD49f+CD90+ cells in the presence or absence of Scriptaid, suggesting that Scriptaid maintains stem cell gene expression programs despite expansion in HSC numbers. (ox.ac.uk)
Supporting this, 50 genes were significantly differentially expressed between CD90+ and CD90- Lin-CD34+CD38-CD45RA-CD49f+ subsets in Scriptaid-cytokine- and cytokine only-expansion conditions. (ox.ac.uk)
e-MSCs supported an enhanced expansion of CD34 + hematopoietic stem and progenitor cells, and generation of myeloid lineage cells in vitro. (elsevierpure.com)
Materials and Methods: We studied the effects of stress, cortisol, antisense oligonucleotides to AChE, and synthetic ARP on peripheral blood cell composition and clonogenic progenitor status in mice under normal and stress conditions, and on purified CD34 + cells of human origin. (tau.ac.il)
In human CD34 + hematopoietic progenitor cells, cortisol elevated AChE-R mRNA levels and promoted hematopoietic expansion. (tau.ac.il)

Researchers at St. Jude have discovered that overexpression of a truncated form of the human HMGA2 gene in a lentiviral vector can cause transduced hematopoietic stem cells to proliferate in transplanted animal models. (stjude.org)

Pulp stem cells (PSCs) are able to form ectopic dentin in vitro and in vivo and also to generate a dentine-pulp complex composed of a mineralized matrix, with dentinal tubules that are aligned and filled with odontoblastic prolongations, containing vascularized pulp tissue, in a similar arrangement to that observed in natural dental structures 3,6 . (bvsalud.org)

They could differentiate into bone, cartilage, and a hematopoietic microenvironment. (biomedcentral.com)

Handling, maintenance and understanding of human pluripotent stem cells. (lu.se)
Inducible Pluripotent Stem Cells (iPSC), first developed in late 2007, are stem cells produced by a number of cells that have been re-engineered back to their pluripotent state, The capability to generate somatic cells to iPSCs may provide significant opportunity to develop patient-specific cell lines to better understand human disorders. (platelettargetedtherapeutics.com)
Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)

Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
Efficient in vitro expansion of MSCs is however necessary for their clinical application. (hindawi.com)
MSCs can easily be obtained from various human tissue sources such as the bone marrow, cord blood, placenta, and adipose [ 2 - 5 ]. (hindawi.com)
Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
Additionally, Abedin and King showed that FGF-2 suppresses the cellular senescence of human MSCs [ 17 ]. (hindawi.com)
MSCs naturally reside in a specialized niche in vivo , which mainly consists of the extracellular matrix (ECM). (hindawi.com)
Here, by using a microarray analysis, we demonstrate that human BM mesenchymal stromal/stem cells (MSCs) in an early osteoinductive stage (e-MSCs) are characterized by unique hematopoiesis-associated gene expression with an enhanced hematopoiesis-supportive ability. (elsevierpure.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
One of the reasons of such outcomes is non-standardized and often disadvantageous ex vivo manipulation of MSCs prior therapy. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Because of their multi/pluripotency and immunosuppressive properties, mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair. (biomedcentral.com)
Producing cells according to Good Manufacturing Practices (GMPs) is a global challenge for the production of all cells for use in humans, specifically mesenchymal stem/stroma cells (MSCs), one of the most promising tools in cellular therapy and regenerative medicine. (biomedcentral.com)
MSCs with a mesodermic origin were first described by Alexander Friedenstein and colleagues in the 1960s and 1970s[ 1 ] as non-hematopoietic bone marrow (BM) cells that adhered to plastic and that could develop into colonies with a fibroblastic appearance. (biomedcentral.com)
A fundamental question in the study of MSCs is that the bona fide identity of human MSCs in vivo is not well defined. (bjmu.edu.cn)
The explicit alteration of fresh cells following ex vivo expansion, as well as the demonstration of in vivo niche for the MSCs in this work, is critical for guiding us to identify optimal culture conditions for maintaining the original characteristics of in vivo MSCs. (bjmu.edu.cn)
This work not only expands our understanding of human bone marrow MSCs, but also provides great hints for further expansion of primary MSCs in vitro, which would be great help for MSCs-based bone regeneration. (bjmu.edu.cn)
Conclusions: The cells isolated from dental pulp exhibited characteristics compatible with those expected for mesenchymal stem cells (MSCs) and are good candidates for cell therapy applications and tissue bioengineering. (bvsalud.org)

MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)

We target the development of 3D in vitro systems capable of modelling the constitutive tissues of the human Bone Organ. (bourginelab.com)
Biomimicking approaches can be implemented toward reconstitution of the constitutive elements of the human skeletal or hematopoietic tissues. (bourginelab.com)
Results: In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. (123dok.net)
Using immunohistochemistry, we observed HOXD9 protein expression in human brain tumor tissues, including astrocytomas and glioblastomas. (123dok.net)
Likewise, commercial trade in cells, tissues and organs - and even trafficking involving humans who are kidnapped or lured into other countries where they are forced to be "donors" - continues to be a serious problem, particularly in countries with substantial transplant tourism. (who.int)

Evaluation of serum-free culture conditions able to support the ex vivo expansion and engraftment of human hematopoietic stem cells in the human-to-sheep xenograft model. (wakehealth.edu)
The hematopoietic retrodifferentiated stem cells have been shown to engraft an animal host in two proofs of principle clinical studies, demonstrating long-term engraftment and safety in acquired aplastic anaemia, while transient amelioration of beta thalassemia major was also observed. (eurekaselect.com)

For several of the human tu- and various assays demonstrated an sues. (who.int)

The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. (stemcell.com)
Therapy-resistant leukemia stem and progenitor cells (LSC) are a main cause of acute myeloid leukemia (AML) relapse. (bvsalud.org)

Dominique Bonnet performs multidisciplinary study in the fields of Stem cell and Progenitor cell via his papers. (research.com)

leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. (stemcell.com)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
Conclusion: The present study showed that garcinol can improve ex vivo expansion of HSCs and enhance their potential for homing to bone marrow. (civilica.com)
This turned out to be the case for both cord blood derived and adult bone marrow stem cells, with LSD1 inhibition expanding stem and progenitor cells from different sources and different stages of development. (lu.se)
With his scientific publications, his incorporates both Bone marrow and Stem cell. (research.com)
The Platelet-Targeted gene transfer treatment involves a series of steps whereby hematopoietic stem cells (HSC) are mobilized with cytokines to permit HSC to travel from bone marrow into the blood, which allows the HSC to be collected by apheresis. (platelettargetedtherapeutics.com)
Currently, we have collaborations with a number of universities to investigate potential clinical applications of lentivector-based stem cells in several areas, such as ischemia, angiogenesis, growth factors, myocardial tissue, coagulation, bone fracture, and promotion of immunity against bacterial and viral pathogens. (platelettargetedtherapeutics.com)
and single cell RNA-seq technology helped us to gain a global recognition of the heterogeneous populations in the murine bone marrow (BM), however, the cell census of human fetal BM remains underestimated owing to material limitations. (bjmu.edu.cn)
Recently, the team published a research paper titled 'Characteristics of Dental Stem Cells in Human Fetal Bone Marrow by Single Cell Transcriptive and Functional Analysis' in the 'Signal Transmission and Targeted Therapy (STTT)', we reported the expression landscape of human fetal BM nucleated cells (BMNCs) based on the single-cell transcriptomic analysis. (bjmu.edu.cn)

We have identified several new growth factors, and established a very potent cell culture system for growing human HSCs. (gabriellesangels.org)
Objective: The application of human cord blood (hCB) is limited to children by using relatively small volume of cord blood that does not contain enough hematopoietic stem cells (HSCs). (civilica.com)
The optimal dose of garcinol for ex vivo expansion of HSCs was determined by direct counting. (civilica.com)
Results: Our results showed that CD133+ HSCs in the presence of garcinol (5-10 µM) had high expansion activity and cell counting showed that the number of cells in treated group was higher than control group (1.9 -fold) and CFC assay showed that the number of colonies following treatment with garcinol had 1.3-fold increase. (civilica.com)
SAGA complex member expression is significantly reduced in aged HSCs and upregulated with heterochronic parabiosis, suggesting a novel mechanism of age-associated hematopoietic decline and rejuvenation. (biorxiv.org)

A) The biological activity of Human Recombinant G-CSF, ACF was tested by its ability to promote the proliferation of mouse NFS-60 cells. (stemcell.com)
Conclusions: Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. (123dok.net)
Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells: impact on in vivo tumor growth. (research.com)

Priming haematopoietic stem/progenitor cells (HSPCs) in vitro with specific chromatin modifying agents and cytokines under serum-free-conditions significantly enhances engraftable HSC numbers. (ox.ac.uk)
We extend these studies by culturing human CD133+ HSPCs on nanofibre scaffolds to mimic the niche for 5-days with the HDAC inhibitor Scriptaid and cytokines. (ox.ac.uk)

To gain insights into how targeting LSD1 led to the expansion of stem cells, the group performed gene expression profiling. (lu.se)
Loss of Tada2b or Taf5l inhibited hematopoiesis in vivo and was associated with upregulation of interferon response gene expression. (biorxiv.org)
Based on principal component analysis and gene expression profiling we demonstrate that CD34+ HSPCs that do not undergo ex vivo expansion are transcriptionally similar to minimally differentiated AML blasts. (ox.ac.uk)

2016). Interleukin-27 early impacts Leishmania infantum infection in mice and correlates with active visceral disease in humans . (up.pt)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)
For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)

Melinda Tursky has specialised in the field of normal and malignant human hematopoiesis and has a strong translational interest in the pathogenesis of hematological cancers. (edu.au)
To address this knowledge gap, we developed an in vivo hematopoietic stem cell (HSC)-based large-scale CRISPR knockout screening platform to enable the genetic interrogation of hematopoiesis and broad aspects of immune cell function in vivo. (biorxiv.org)

Researchers from Lund Stem Cell Center have identified a novel target for the expansion of human blood stem cells ex vivo and shed light on the mechanism of action of a stem cell promoting molecule, currently in phase II clinical trials. (lu.se)
They compared LSD1 inhibition to treatment with UM171, a human hematopoietic stem cell promoting molecule identified in 2014 and currently in phase II clinical trials, despite an as yet unidentified mechanism of action. (lu.se)
Conclusions: Our findings attribute a role to AChE-R and ARP in hematopoietic homeostasis following stress, and suggest the use of ARP in clinical settings where ex vivo expansion of progenitor cells is required. (tau.ac.il)
High throughput proteomic data facilitated the understanding of various biological systems with respect to normal and pathological conditions particularly in the instances of human clinical manifestations. (oaji.net)
The Cell Manipulations Laboratory's experienced scientists can help you develop and scale up your ex-vivo gene transfer and cell therapy clinical trials or simply transfer your existing at-scale technology to our clean facilities which will enable you to move your research to a safe and effective patient treatment plan. (cincinnatichildrens.org)
2017). Pre-clinical antigenicity studies of an innovative multivalent vaccine for human visceral leishmaniasis . (up.pt)
In summary, an in vivo zebra fish imaging screen can be useful to identify potential drug candidates with clinical relevance. (stemcell-lab-mk1dresden.de)
Using a lentivector, we have performed pre-clinical studies using tissue cultured human cells, murine and canine models for both Hemophilia A and Glanzmann Thrombasthenia and have entered Phase I clinical trials in Hemophilia A patients with a history of inhibitory antibodies to recombinant and plasma derived Factor VIII infusion therapy. (platelettargetedtherapeutics.com)
Building off experience in hematopoietic stem cell transplant and exploiting advances in genetic engineering, genome editing, immunology, immunotherapy, stem cell biology, understanding disease mechanisms and cell therapy manufacturing, considerable progress has been made with hundreds of clinical trials underway. (bdbiosciences.com)
The ease by which various stem cell types can be generated from human peripheral blood has allowed the design of various kits to guarantee the specificity, sterility and efficacy of stem cells production for various clinical and research applications. (eurekaselect.com)

called either cancer stem cells (CSCs) or tumor-initiat-ing cells[3]. (123dok.net)
Lung tumor stem cells are a subpopulation of cells important for lung tumor development, metastasis, and medication resistance. (immune-source.com)
After that, tumor control cells had been reported in different types of malignancies, including lung tumor, gastric tumor, human brain cancers, liver organ cancers, and digestive tract cancers [7C11]. (immune-source.com)
Evidence exists that immune activation against hematopoietic elements frequently occurs in MDS patients, based on the identification of lymphocytic infiltrates in the marrow, oligoclonal expansion of T cells, and excessive production of tumor necrosis factor alpha. (acgtfoundation.org)
1. Examine the in vivo effect of: a) DNA methyltransferase inhibitors, b) HDACi, and c) IMiDs on the response to GM-CSF tumor vaccines in a mouse model (year 1). (acgtfoundation.org)

When grown outside the body hematopoietic stem cells proliferate and differentiate from an immature state to more mature blood cell types. (lu.se)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)

Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
Better understanding of the cellular and molecular pathophysiological mechanisms regulating adipocyte size, number and depot-dependent expansion has become a focus of interest over recent decades. (springer.com)

Cell Stem Cell 2018. (bourginelab.com)
During 2003-2018, an average of 8 (range 4-21) EEE cases/year in humans were reported to the Centers for Disease Control and Prevention (CDC) ( 8 ). (cdc.gov)

This was a very exciting result as indicated that we are in the right track and identified factors that can reprogram blood vessels to bud blood stem cells. (ca.gov)

However, the relative low transduction efficiency in human long-term hematopoietic stem cells limits the utility of these vectors in this cell type. (stjude.org)
T cells are modified ex vivo by transduction with viral vectors containing CAR or other modifications of interest. (bdbiosciences.com)

In 2019, an unprecedented epidemic of EEE across the eastern and upper midwestern United States resulted in 38 confirmed cases in humans, most in Massachusetts and Michigan ( 8 ). (cdc.gov)

Lentivector insertion into the genomic DNA has a very low probability to lead to insertional mutagenesis, clonal expansion, or cancer. (platelettargetedtherapeutics.com)
Thought to be disorders of hematopoietic stem cells, clonal cytogenetic abnormalities are frequently identified. (acgtfoundation.org)

Postdoctoral researcher Agatheeswaran Subramaniam and PhD Student Kristijonas Žemaitis from the Stem Cell Regulators research group. (lu.se)

2017). Infection of hematopoietic stem cells by Leishmania infantum increases erythropoiesis and alters the phenotypic and functional profiles of progeny . (up.pt)
Moreover, The use of animals as surrogate rine host, can provide a platform for animal models for tumour viruses in hosts for the study of human tu- in vivo infection. (who.int)

Stem and leukemic environmental stress responses. (imperial.ac.uk)
Mathematical modeling of stem and leukemic cell bioprocessing. (imperial.ac.uk)
Analysis of leukemia stem cell (LSC) chromatin changes at relapse indicated that this leukemic compartment underwent significantly less epigenetic evolution than non-LSCs, while epigenetic changes in non-LSCs reflected overall evolution of the bulk leukemia. (bvsalud.org)

2015). Drug discovery for human African trypanosomiasis: Identification of novel scaffolds by the newly developed HTS SYBR green assay for trypanosoma brucei . (up.pt)

We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
Similar expansions could also be obtained from the peripheral blood of patients with acute leukemia undergoing therapy ( n = 11). (aacrjournals.org)
SIGNIFICANCE: The elimination of therapy-resistant leukemia stem and progenitor cells (LSC) remains a major challenge in the treatment of AML. (bvsalud.org)
Anti-CD38 antibody-mediated clearance of human repopulating cells masks the heterogeneity of leukemia-initiating cells. (research.com)
Leukemia is characterized by the abnormal and uncontrolled expansion of malfunctioning blood cells that crowd out normal cells. (lu.se)

We aim to identify novel ways to counteract this maturation, pushing stem cells to self-renew and increase in numbers. (lu.se)

During this process, leukocytes lose lineage-associated markers home and undergo homocytic aggregation, upregulate expression of stem cell antigens, and subsequently redifferentiate to give rise original tissue or, transdifferentiate into a different tissue altogether. (eurekaselect.com)
Binding of MHC class II antigens on leukocytes with the monoclonal antibody CR3/43 appears to emulate stress and injury in human tissue in vitro, similar to limb amputation in salamander. (eurekaselect.com)
The robustness and efficacy of the retrodifferentiation process in generating unprecedented quantities of stem cells belonging to the three germ layers will enable organ and tissue reconstruction ex vivo, using bio-printing and various scaffold materials. (eurekaselect.com)
The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)

Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)

The objective of this proposal was to identify factors that allow blood vessels to generate hematopoietic stem cells early in the embryonic stage. (ca.gov)

2016). Poly-N-acetylglucosamine production by Staphylococcus epidermidis cells increases their in vivo proinflammatory effect . (up.pt)

In addition, we developed a series of novel tools to test the biological relevance of the genes identified in vivo (using mouse embryos). (ca.gov)
Specifically, we have tested whether forced expression of these genes could induce the generation of hematopoietic stem cells. (ca.gov)

The first step was to test whether human endothelial cells could offer a supportive niche for the growth of hematopoietic cells. (ca.gov)

By incorporating the HMGA2 gene, the small number of hematopoietic stem cells that were initially transduced (about 1%) expanded over a period of two years to become 40-70% of the stem cell population in transplant recipients. (stjude.org)
This protocol has been successful in human megakaryocytes (parental cells of platelets) and platelets produced in a xeno‑transplant mouse model. (platelettargetedtherapeutics.com)

Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
FIGURE 2-1 Formation of the multiple peripheral blood cells from multipotent hematopoietic stem cells. (nationalacademies.org)

Anatomy56

Organisms15

Diseases20

Chemicals and Drugs64

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Psychiatry and Psychology1

Phenomena and Processes46

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Named Groups4

Health Care3

Progenitors4

Transplantation15

Differentiation17

CD345

Transduced hematopoietic stem1

Vitro and in vivo1

Microenvironment1

Pluripotent stem3

MSCs18

Stromal cells1

Tissues5

Engraftment2

Assays1

Myeloid2

Progenitor cell1

Bone9

HSCs5

Proliferation3

Cytokines2

Gene expression3

Mice4

Hematopoiesis2

Clinical10

Tumor5

Proliferate2

Cellular2

20182

Blood stem cells1

Transduction2

20191

Clonal2

Subramaniam1

Infection2

Leukemic3

Scaffolds1

Leukemia5

Maturation1

Tissue4

Isolation1

Embryonic1

20161

Genes2

Niche1

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Peripheral blood2