Vivo expansion of hematopoietic stem cells. Medical search. Frequent questions

Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
decidual-like NK cell subset following autologous hematopoietic stem cell transplantation. (bvsalud.org)
In vivo expansion of a CD9 + decidual-like NK cell subset following autologous hematopoietic stem cell transplantation. (bvsalud.org)
Autologous hematopoietic stem cell transplantation (autoHSCT) is a treatment option for hematological disorders and pediatric solid tumors . (bvsalud.org)
The ability to grow blood stem cells (HSCs) in culture would greatly improve the patient outcome in transplantation, and make cord blood to be useful for adult applications. (gabriellesangels.org)
We will further investigate why our cultured cells greatly enhance allogeneic transplantation, and identify the responsible cell populations facilitating the allograft. (gabriellesangels.org)
So, efforts for applying cord blood stem cells in transplantation have led to establishment of some approaches for ex vivo expansion of HSCs such as garcinol. (civilica.com)
Smith and Broxmeyer, 1986), thereby making it a potential source of cells for transplantation (Bodger, 1987). (nationalacademies.org)
In addition, short-term osteoinductive treatment favored in vivo hematopoietic recovery in lethally irradiated mice that underwent BM transplantation. (elsevierpure.com)
Our findings demonstrate the unique biological characteristics of e-MSCs as hematopoiesis-regulatory stromal cells at differentiation stage between MSCs and osteoprogenitor cells and have significant implications in developing new strategy for using pharmacological osteoinductive treatment to support hematopoiesis in hematopoietic stem and progenitor cell transplantation. (elsevierpure.com)
Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation. (ox.ac.uk)
The transplantation of ex vivo expanded mobilized peripheral blood haematopoietic stem cells (PBSC), in place of unmanipulated cells following high dose chemotherapy, reduces the period of cytopenia associated with the therapy's hemotoxicity. (edu.au)
A method is described for transplantation of neonatal mice via facial vein to reconstitute the blood system with hematopoietic stem cells from any source. (wenzellab.com)
Moreover, HSPCs are of Major relevance for the establishment of successful hematopoietic stem cell transplantation (HSCT) strategies. (stemcell-lab-mk1dresden.de)
We have over-expressed SKI to define its intrinsic role in hematopoiesis and myeloid neoplasms, which resulted in a robust competitive advantage upon transplantation, a complete dominance of the stem and progenitor compartments, and a marked enhancement of myeloid differentiation at the expense of other lineages. (edu.au)
Hematopoietic stem cell transplantation is a curative therapy for many patients suffering from life threatening blood disorders. (lu.se)
Although the number of transplants each year has grown rapidly over the past two decades, the demand for transplantation using human cells, tissues and organs has also increased significantly, resulting in a continuing shortage of human material, particularly organs. (who.int)
As few countries are approaching self- sufficiency in the provision of cells, tissues and organs for transplantation, new ways continue to be sought to increase the donation of human material. (who.int)

In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
The clinical application of MSCs to tissue engineering has been introduced due to their numerous advantages including high expansion potential and extensive differentiation potential [ 6 , 7 ]. (hindawi.com)
The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. (stemcell.com)
Hypothesising that Scriptaid plus cytokines expands the CD90+ subset without differentiation and upregulates CD90 on CD90- cells, we sorted, then cultured Lin-CD34+CD38-CD45RA-CD90- cells with Scriptaid and cytokines. (ox.ac.uk)
B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
These primitive cells undergo division and differentiation to form the various peripheral blood cells. (nationalacademies.org)
Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be? (google.se)
HoxB4, a homeodomain-containing transcription factor, is involved in the expansion of hematopoietic stem cells and progenitor cells in vivo and in vitro, and plays a key role in regulating the balance between hematopoietic stem cell renewal and cell differentiation. (kyobobook.co.kr)
Self-renewal and differentiation are tightly controlled to maintain haematopoietic stem cell (HSC) homeostasis in the adult bone marrow 1,2 . (elsevierpure.com)
During fetal development, expansion of HSCs (self-renewal) and production of differentiated haematopoietic cells (differentiation) are both required to sustain the haematopoietic system for body growth 3,4 . (elsevierpure.com)
Accordingly, enforced expression of SKI induced a gene signature associated with hematopoietic stem cells and myeloid differentiation, as well as hepatocyte growth factor signaling. (edu.au)
Mesenchymal cells), with possibility to induce their differentiation. (bourginelab.com)
In a study published in the journal Blood, the Larsson group targeted the enzyme LSD1, part of the CoREST complex, known to mediate the epigenetic modification of DNA and pushing the differentiation of stem cells into mature blood cells. (lu.se)
We hypothesised that by reducing LSD1 levels using small molecule drugs we could halt stem cell differentiation and stimulate expansion. (lu.se)
These observations imply that current in vitro differentiation strategies are missing critical cues which are essential to unlock or maintain full HSC potential in vivo. (grantome.com)
Defining the molecular signaling pathways that mediate productive HSC formation in vivo will reveal new targets for optimizing the directed expansion and/or differentiation of adult-type HSCs for clinical use. (grantome.com)
In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
Objective: To characterize pulp stem cells and evaluate their capacity for expansion and differentiation in vitro. (bvsalud.org)
Results: It was observed that the pulp stem cells exhibited the capacity to adhere to plastic and a high rate of expansion and, after detection with specific stains, it was shown that the cells were capable of differentiation into osteoblasts and chondroblasts, but not into adipocytes. (bvsalud.org)
Stem cells are cells with a low degree of differentiation that have the capacity to reproduce and can generate differentiated cells of several different types of tissues 1 . (bvsalud.org)
The objective of this study is to isolate and characterize stem cells from the pulp of permanent third molars and analyze the cells obtained in terms of their morphology and chondrogenic, osteogenic and adipogenic cell differentiation. (bvsalud.org)

The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Single-cell assessment of transcriptome alterations induced by Scriptaid in early differentiated human haematopoietic progenitors during ex vivo expansion. (ox.ac.uk)
Indeed, one critical question contemplated at the Workshop was whether tumors derive from organ stem cells that retain self-renewal properties but acquire epigenetic and genetic changes required for tumorigenicity or whether tumor stem cells are proliferative progenitors that acquire self-renewal capacity. (aacrjournals.org)
Here we used in vivo genetic tracing in mice to analyse the formation of HSCs and progenitors from intra-arterial haematopoietic clusters, which contain HSC precursors and express the transcription factor hepatic leukaemia factor (HLF). (elsevierpure.com)
Through kinetic study, we observed the simultaneous formation of HSCs and defined progenitors-previously regarded as descendants of HSCs 5 -from the HLF + precursor population, followed by prompt formation of the hierarchical haematopoietic population structure in the fetal liver in an HSC-independent manner. (elsevierpure.com)
These data suggest that fetal HSCs minimally contribute to the generation of progenitors and functional blood cells before birth. (elsevierpure.com)
Using a mouse model of Mir223 deletion we found that loss of this gene results in a modest expansion of myeloid progenitors without significant effects on hematopoietic stem cell (HSC) function or development of malignancy with age. (wustl.edu)

Subjecting transduced HSCs to an ex vivo expansion protocol following the transduction procedure could partially reverse this loss. (ox.ac.uk)
CONCLUSIONS: These studies suggest that loss of repopulating ability of transduced HSCs rather than low gene transfer efficiency might be the main problem in clinical gene therapy protocols, and that a clinically feasible ex vivo expansion approach post-transduction can markedly improve reconstitution with gene marked stem cells. (ox.ac.uk)
Hematopoietic stem cells (HSCs) are the stem cells that give rise to other blood cells. (wikipedia.org)
In vertebrates, the very first definitive HSCs arise from the ventral endothelial wall of the embryonic aorta within the (midgestational) aorta-gonad-mesonephros region, through a process known as endothelial-to-hematopoietic transition. (wikipedia.org)
SAGA complex member expression is significantly reduced in aged HSCs and upregulated with heterochronic parabiosis, suggesting a novel mechanism of age-associated hematopoietic decline and rejuvenation. (biorxiv.org)
We have identified several new growth factors, and established a very potent cell culture system for growing human HSCs. (gabriellesangels.org)
In steady-state adult hematopoiesis, most hematopoietic stem cells (HSCs) are in the resting phase of the cell cycle. (uzh.ch)
Upon enhanced hematopoietic demand, HSCs can be induced to divide and self-renew or differentiate. (uzh.ch)
Using in vivo high-resolution single HSC divisional tracking, we directly demonstrate that clinically applied thrombopoietin receptor but not granulocyte colony-stimulating factor (G-CSF) receptor agonists drive HSCs into self-renewing divisions leading to quantitative expansion of functional HSC as defined by their in vivo serial multilineage and long-term repopulating potential. (uzh.ch)
These results suggest that thrombopoietin mimetics might be applicable to expand HSCs in vivo and to sensitize thrombopoietin receptor-expressing HSCs to cell cycle-dependent cytotoxic agents. (uzh.ch)
Objective: The application of human cord blood (hCB) is limited to children by using relatively small volume of cord blood that does not contain enough hematopoietic stem cells (HSCs). (civilica.com)
Materials and Methods: CD133+ HSCs were separated by a magnetic-activated cell sorting (MACS) system. (civilica.com)
The optimal dose of garcinol for ex vivo expansion of HSCs was determined by direct counting. (civilica.com)
Results: Our results showed that CD133+ HSCs in the presence of garcinol (5-10 µM) had high expansion activity and cell counting showed that the number of cells in treated group was higher than control group (1.9 -fold) and CFC assay showed that the number of colonies following treatment with garcinol had 1.3-fold increase. (civilica.com)
Conclusion: The present study showed that garcinol can improve ex vivo expansion of HSCs and enhance their potential for homing to bone marrow. (civilica.com)
Haematopoietic stem cells (HSCs) support blood system homeostasis and are also used clinically in cell and gene therapies. (ox.ac.uk)
Multipotent and self-renewing HSCs sustain our haematopoietic systems throughout life. (ox.ac.uk)
Towards this goal, we recently established methods to expand transplantable HSCs long-term ex vivo (Wilkinson et al. (ox.ac.uk)
Cas9-AAV6 gene correction of beta-globin in autologous HSCs improves sickle cell disease erythropoiesis in mice. (ox.ac.uk)
The transcription factor EVI1 is heterogeneously expressed within the precursor population, with EVI1 hi cells being predominantly localized to intra-embryonic arteries and preferentially giving rise to HSCs. (elsevierpure.com)
Hematopoietic stem cells (HSCs), first produced in the developing vertebrate embryo, supply the lifelong foundation of the blood and immune systems. (grantome.com)
Likewise, while the number of transplanted HSCs is well known to directly impact engraftment efficiency, there are currently no established clinical protocols to successfully expand donor-harvested HSCs, nor to differentiate embryonic or induced pluripotent stem cells (iPSCs) into functional HSCs in vitro. (grantome.com)
In the developing embryo, definitive HSCs arise from a unique population of mesodermal precursors termed hemogenic endothelium (HEC) through a process known as endothelial-to-hematopoietic transition (EHT). (grantome.com)
Hematopoietic stem cells (HSCs), produced in the developing embryo, form the foundation of our blood system. (grantome.com)
This work has significant relevance for the in vitro production and expansion of functional HSCs for therapeutic use. (grantome.com)
Concordant with this, we found that Tet2 deletion conferred a strong functional competitive advantage to Jak2V617F-mutant hematopoietic stem cells (HSCs). (westminster.ac.uk)
In aggregate, our findings indicate that Tet2 loss drives clonal dominance in HSCs, and Jak2V617F expression causes expansion of downstream precursor cell populations, resulting in disease progression through combinatorial effects. (westminster.ac.uk)

However, the relative low transduction efficiency in human long-term hematopoietic stem cells limits the utility of these vectors in this cell type. (stjude.org)

MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
Samples: Human bone marrow mesenchymal stromal cells exposed to static or laminar shear stress conditions. (wenzellab.com)

We searched the databases such as EMBASE, PubMed, and Web of Science with the keywords "stem cell," "progenitor cell," "satellite," "senescence" and excluded the keywords "cancer," "tumor," "malignancy" and "carcinoma" until June 2020. (magiran.com)

Our investigation indicates that most of these studies examined endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells, and a few others were about less-discussed types of stem cells such as cardiac stem cells, myeloblasts, and induced pluripotent stem cells. (magiran.com)
Handling, maintenance and understanding of human pluripotent stem cells. (lu.se)
Inducible Pluripotent Stem Cells (iPSC), first developed in late 2007, are stem cells produced by a number of cells that have been re-engineered back to their pluripotent state, The capability to generate somatic cells to iPSCs may provide significant opportunity to develop patient-specific cell lines to better understand human disorders. (platelettargetedtherapeutics.com)
Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)

Ex vivo expansion of cord blood cells. (civilica.com)
Umbilical cord blood is a rich source of these committed progenitor cells and, presumably, multipotent HPCs (Knudtzon, 1974). (nationalacademies.org)
Process optimization was continued using cord blood (CB) CD34+ cells. (edu.au)
This approach has the advantages of rapid availability and reduced stringency for a complete donor/recipient match, but is limited by a relatively low number of stem cells within a single cord blood unit. (lu.se)
This turned out to be the case for both cord blood derived and adult bone marrow stem cells, with LSD1 inhibition expanding stem and progenitor cells from different sources and different stages of development. (lu.se)

In vertebrates, the vast majority of hematopoiesis occurs in the bone marrow and is derived from a limited number of hematopoietic stem cells that are multipotent and capable of extensive self-renewal. (wikipedia.org)
To address this knowledge gap, we developed an in vivo hematopoietic stem cell (HSC)-based large-scale CRISPR knockout screening platform to enable the genetic interrogation of hematopoiesis and broad aspects of immune cell function in vivo. (biorxiv.org)
Loss of Tada2b or Taf5l inhibited hematopoiesis in vivo and was associated with upregulation of interferon response gene expression. (biorxiv.org)
Here, by using a microarray analysis, we demonstrate that human BM mesenchymal stromal/stem cells (MSCs) in an early osteoinductive stage (e-MSCs) are characterized by unique hematopoiesis-associated gene expression with an enhanced hematopoiesis-supportive ability. (elsevierpure.com)
Noteworthy, expression of the hematopoiesis-associated molecules CXCL12 and vascular cell adhesion molecule 1 was remarkably decreased in e-MSCs. (elsevierpure.com)
Transplant studies showed that deletion of Mir142 together with over-expression of mutant IDH2 resulted in anemia and neutrophilia in recipient mice, suggesting these mutations cooperate in vivo to induce aberrant hematopoiesis. (wustl.edu)
Collectively these data further support the importance of sncRNAs in the maintenance of the hematopoietic system and reveal how their dysregulation can lead to aberrant hematopoiesis. (wustl.edu)

A) The biological activity of Human Recombinant G-CSF, ACF was tested by its ability to promote the proliferation of mouse NFS-60 cells. (stemcell.com)
Cell proliferation was measured using a fluorometric assay method. (stemcell.com)
The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. (stemcell.com)
Here, in the presence of KL+FL+megakaryocyte growth and development factor (MGDF), which recruits virtually all Lin(-)Sca-1(+)kit(+) bone marrow cells into proliferation and promotes their self-renewal under serum-free conditions, IL-3 and IL-11 revealed an indistinguishable ability to further enhance proliferation. (lu.se)
Analysis of phenotypical characteristics showed that HoxB4 overexpression in Ba/F3 cells reduced cell size, death, and proliferation rate. (kyobobook.co.kr)
Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. (thermofisher.com)
The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. (thermofisher.com)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)

Evaluation of serum-free culture conditions able to support the ex vivo expansion and engraftment of human hematopoietic stem cells in the human-to-sheep xenograft model. (wakehealth.edu)

e-MSCs exhibited the absence of decreased stemness-associated gene expression, increased osteogenesis-associated gene expression, and apparent mineralization, thus maintaining the ability to differentiate into adipogenic cells. (elsevierpure.com)
These cancer stem cells have the capacity to both divide and expand the cancer stem cell pool and to differentiate into the heterogeneous nontumorigenic cancer cell types that in most cases appear to constitute the bulk of the cancer cells within the tumor. (aacrjournals.org)
When grown outside the body hematopoietic stem cells proliferate and differentiate from an immature state to more mature blood cell types. (lu.se)
GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)

The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. (stemcell.com)
Use of polyvinyl alcohol for chimeric antigen receptor T-cell expansion. (ox.ac.uk)

Despite using optimized culture conditions, transduction resulted in striking losses of repopulating activity, translating into low numbers of gene marked cells in competitively repopulated mice. (ox.ac.uk)
Another CFU, the colony-forming unit-spleen (CFU-S), was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. (wikipedia.org)
To dissect the effects of concomitant Jak2V617F expression and Tet2 loss within distinct hematopoietic compartments in vivo, we generated Jak2V617F/Tet2 compound mutant genetic mice. (westminster.ac.uk)
To model this in vivo, we characterized the hematopoietic compartment of mice lacking the Mir142 gene. (wustl.edu)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

In striking contrast, the ex vivo expansion of murine HSC in fetal calf serum-containing medium resulted in extensive loss of reconstituting activity, an effect further facilitated by the presence of IL-3. (lu.se)
Samples: Doxycline-inducible intracellular Notch1 domain (ICN1) murine embryonic stem cells differentiated for 3-5 days with or without induction of Notch1, followed by fluorescence-activated cell sorting for surface expression of VE-cadherin and CD41. (wenzellab.com)
The proto-oncogene SKI is highly expressed in human myeloid leukemia and also in murine hematopoietic stem cells. (edu.au)
Using a lentivector, we have performed pre-clinical studies using tissue cultured human cells, murine and canine models for both Hemophilia A and Glanzmann Thrombasthenia and have entered Phase I clinical trials in Hemophilia A patients with a history of inhibitory antibodies to recombinant and plasma derived Factor VIII infusion therapy. (platelettargetedtherapeutics.com)

In shape, hematopoietic stem cells resemble lymphocytes. (wikipedia.org)
This was associated with increased infiltration of macrophages, lymphocytes, and mast cells. (oaji.net)
Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)

The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)
Pulp stem cells (PSCs) are able to form ectopic dentin in vitro and in vivo and also to generate a dentine-pulp complex composed of a mineralized matrix, with dentinal tubules that are aligned and filled with odontoblastic prolongations, containing vascularized pulp tissue, in a similar arrangement to that observed in natural dental structures 3,6 . (bvsalud.org)

citation needed] Hematopoietic stem cells can be identified or isolated by the use of flow cytometry where the combination of several different cell surface markers (particularly CD34) are used to separate the rare Hematopoietic stem cells from the surrounding blood cells. (wikipedia.org)
There was no significant difference in the transcriptomic profile, by RNAsequencing, between cytokine-expanded and purified Lin-CD34+CD38-CD45RA-CD49f+CD90+ cells in the presence or absence of Scriptaid, suggesting that Scriptaid maintains stem cell gene expression programs despite expansion in HSC numbers. (ox.ac.uk)
Supporting this, 50 genes were significantly differentially expressed between CD90+ and CD90- Lin-CD34+CD38-CD45RA-CD49f+ subsets in Scriptaid-cytokine- and cytokine only-expansion conditions. (ox.ac.uk)
e-MSCs supported an enhanced expansion of CD34 + hematopoietic stem and progenitor cells, and generation of myeloid lineage cells in vitro. (elsevierpure.com)

The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
FIGURE 2-1 Formation of the multiple peripheral blood cells from multipotent hematopoietic stem cells. (nationalacademies.org)
Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)

Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)

Colony forming cell (CFC) assay was performed to evaluate clonogenic capability of treated cells. (civilica.com)

However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
Stem cell-independent pathways during development thus offer a rational strategy for the rapid and simultaneous growth of tissues and stem cell pools. (elsevierpure.com)
Biomimicking approaches can be implemented toward reconstitution of the constitutive elements of the human skeletal or hematopoietic tissues. (bourginelab.com)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
Likewise, commercial trade in cells, tissues and organs - and even trafficking involving human beings who are kidnapped or lured into other countries where they are forced to be "donors" - continues to be a serious problem, particularly in countries with substantial transplant tourism. (who.int)

This has triggered the search for the optimal combination of cytokines for HSC expansion. (lu.se)
Priming haematopoietic stem/progenitor cells (HSPCs) in vitro with specific chromatin modifying agents and cytokines under serum-free-conditions significantly enhances engraftable HSC numbers. (ox.ac.uk)
The Platelet-Targeted gene transfer treatment involves a series of steps whereby hematopoietic stem cells (HSC) are mobilized with cytokines to permit HSC to travel from bone marrow into the blood, which allows the HSC to be collected by apheresis. (platelettargetedtherapeutics.com)
During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)

Researchers at St. Jude have discovered that overexpression of a truncated form of the human HMGA2 gene in a lentiviral vector can cause transduced hematopoietic stem cells to proliferate in transplanted animal models. (stjude.org)

2017). Infection of hematopoietic stem cells by Leishmania infantum increases erythropoiesis and alters the phenotypic and functional profiles of progeny . (up.pt)
2017). Leishmania infantum exoproducts inhibit human invariant NKT cell expansion and activation . (up.pt)

Myeloid and lymphoid lineages both are involved in dendritic cell formation. (wikipedia.org)
Hematopoietic stem and progenitor cells (HSPCs) are crucial for the maintenance of homeostasis of all blood lineages in human. (stemcell-lab-mk1dresden.de)
This widespread expression of CD117 in hematopoietic precursors is consistent with the participation of c-Kit and its ligand in the regulation of several hematopoietic lineages. (bdbiosciences.com)

article{ff9b980d-2e89-453c-b22b-428d2e903c6f, abstract = {{Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)

Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)

Neutrophils undergo apoptosis and are then cleared by macrophages or other phagocytic cells, which clear the toxic contents. (medscape.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Hematopoietic stem cells can replenish all blood cell types (i.e., are multipotent) and self-renew. (wikipedia.org)
Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)
In the cancer stem cell model of tumors, there is a small subset of cancer cells, the cancer stem cells, which constitute a reservoir of self-sustaining cells with the exclusive ability to self-renew and maintain the tumor. (aacrjournals.org)
We aim to identify novel ways to counteract this maturation, pushing stem cells to self-renew and increase in numbers. (lu.se)

RESULTS: We demonstrate that conditions supporting in vitro stem cell self-renewal efficiently promote oncoretroviral-mediated gene transfer to multipotent adult bone marrow stem cells, without prior in vivo conditioning. (ox.ac.uk)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
Especially in cases where sufficient cells cannot be obtained due to the rarity of the disorder, iPSC models have shown great potential in providing research material to develop new treatments or diagnostics, but also to study the pathophysiology of the diseases [ 10 , 12 ]. (biomedcentral.com)

Lentivector insertion into the genomic DNA has a very low probability to lead to insertional mutagenesis, clonal expansion, or cancer. (platelettargetedtherapeutics.com)

The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. (hindawi.com)
MSCs naturally reside in a specialized niche in vivo , which mainly consists of the extracellular matrix (ECM). (hindawi.com)

Proteomics is a powerful approach to study the whole set of proteins expressed in an organism, organ, tissue or cell resulting in valuable information on physiological or pathological state of a biological system. (oaji.net)
When an accumulation of unfolded or misfolded proteins occurs in the lumen of the endoplasmic reticulum, the UPR works to protect the cell against the damage caused by these improperly folded proteins. (medscape.com)

Lack of expression of lineage markers is used in combination with detection of several positive cell-surface markers to isolate hematopoietic stem cells. (wikipedia.org)
CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. (thermofisher.com)

BACKGROUND: Extensive efforts to develop hematopoietic stem cell (HSC) based gene therapy have been hampered by low gene marking. (ox.ac.uk)
Perinatal Cells: A Promising COVID-19 Therapy? (unicatt.it)
The role of b cells in pe pathophysiology: A potential target for perinatal cell-based therapy? (unicatt.it)
The Cell Manipulations Laboratory's experienced scientists can help you develop and scale up your ex-vivo gene transfer and cell therapy clinical trials or simply transfer your existing at-scale technology to our clean facilities which will enable you to move your research to a safe and effective patient treatment plan. (cincinnatichildrens.org)
Researchers and clinicians can contact us for more information about how we can help you with your cell therapy and gene transfer needs. (cincinnatichildrens.org)
In addition to the aging phenomenon, stem cell senescence has been investigated in various concepts such as cancer, adverse drug effects, and as a limiting factor in cell therapy. (magiran.com)
Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
From that effort, we have developed an ex-vivo lentiviral-based gene therapy technology that isolates the patient's own stem cells, then introduces a normal gene to replace defective genetic material into those cells, which, after being incorporated into the patient's stem cells, functionally generates modified cells specifically designed to eradicate the genetic disease. (platelettargetedtherapeutics.com)
An ongoing concern with systemic delivery of chemotherapy has been that such therapy can have deleterious and/or off-target effects on healthy cells. (platelettargetedtherapeutics.com)
Conclusions: The cells isolated from dental pulp exhibited characteristics compatible with those expected for mesenchymal stem cells (MSCs) and are good candidates for cell therapy applications and tissue bioengineering. (bvsalud.org)

In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. (stemcell.com)
The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)

Hematopoietic stem cells constitute 1:10,000 of cells in myeloid tissue. (wikipedia.org)
If the cancer stem cell model is correct and if such cells retain the hallmarks of some tissue stem cells in being rare and entering the cell cycle infrequently, they could constitute a population that is intrinsically resistant to current therapies designed to kill cycling cells. (aacrjournals.org)
CML offers more-than-minimally manipulated cell and tissue procedures performed aseptically in our cleanroom in accordance with GMP / GTP quality compliance requirements . (cincinnatichildrens.org)
CellPlan Limited ('CellPlan'), which has developed the world's first stem cell healthcare insurance plan and medical concierge service, has cemented its agreement with Biovault Technical Ltd ('Biovault'), the UK's largest private human tissue storage facility via a definitive agreement. (bioinformant.com)
Currently, we have collaborations with a number of universities to investigate potential clinical applications of lentivector-based stem cells in several areas, such as ischemia, angiogenesis, growth factors, myocardial tissue, coagulation, bone fracture, and promotion of immunity against bacterial and viral pathogens. (platelettargetedtherapeutics.com)
The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
Methods: Pulp tissue was collected from permanent third molars and digested and then the cells were seeded onto plates containing HDMEM medium. (bvsalud.org)

This approach can be used to achieve in vivo expansion of hematopoietic stem cells transduced with lentiviral vectors that carry any desired therapeutic gene, such as gamma-globin, or to enhance genome editing efficiency. (stjude.org)

First, we looked at LIN28B, a gene recognized as the master regulator of fetal blood cell formation. (lu.se)

leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. (stemcell.com)

By genetically manipulating EVI1 expression, we were able to alter HSC and progenitor output from precursors in vivo. (elsevierpure.com)

2016). Poly-N-acetylglucosamine production by Staphylococcus epidermidis cells increases their in vivo proinflammatory effect . (up.pt)
In a recent peer-review article published in the journal Xenotransplantation (September 2016), AltuCell's medical lead Dr. Giovanni Luca reported a preclinical breakthrough utilizing microencapsulated Sertoli cells to establish reversal of diabetes in a mouse model. (bioinformant.com)

We are studying the biology of this important stem cell population with the aim of developing new HSC-based therapies. (ox.ac.uk)

Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
Efficient in vitro expansion of MSCs is however necessary for their clinical application. (hindawi.com)
In comparison to BM-MSCs without osteoinductive treatment, gene expression in e-MSCs was significantly altered in terms of their cell adhesion- and chemotaxis-related profiles, as identified with Gene Ontology and Gene Set Enrichment Analysis. (elsevierpure.com)
It was a interesting week in the stem cell sector, with new releases about MSCs, exosomes, Sertoli cells, and more. (bioinformant.com)

It will also help quantify the percentage of the cancer cells in the marrow and help with prognostication by seeking the genetic change that the cancer cells harbour. (singhealthdukenus.com.sg)
Since these mutations occur in utero and are very hard to study in humans, we used mouse models that express the same genetic mutations to investigate their impact on the blood or hematopoietic system. (lu.se)

It was used extensively in early studies, but is now considered to measure more mature progenitor or transit-amplifying cells rather than stem cells[citation needed]. (wikipedia.org)

The goals of the UPR are to maintain homeostasis in the cell by arresting protein translation and promoting signaling pathways that lead to increased production of molecular chaperones to help with protein folding. (medscape.com)

By using multiparametric flow cytometry , we confirmed the expansion of NK cells expressing CD9 immediately after autoHSCT, which exhibited higher granzyme B and perforin expression levels than CD9- NK cells . (bvsalud.org)
Flow cytometry analysis of CD19 was performed by staining Raji CD19 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ (Product # 12-0199-42, blue histogram). (thermofisher.com)

Researchers from Lund Stem Cell Center have identified a novel target for the expansion of human blood stem cells ex vivo and shed light on the mechanism of action of a stem cell promoting molecule, currently in phase II clinical trials. (lu.se)
They compared LSD1 inhibition to treatment with UM171, a human hematopoietic stem cell promoting molecule identified in 2014 and currently in phase II clinical trials, despite an as yet unidentified mechanism of action. (lu.se)

Postdoctoral researcher Agatheeswaran Subramaniam and PhD Student Kristijonas Žemaitis from the Stem Cell Regulators research group. (lu.se)

Results showed profound changes in the gene expression profile of NK cells immediately after autoHSCT. (bvsalud.org)
Significantly, we observed that following autoHSCT, NK cells acquired a decidual-like gene expression profile , including the expression of CD9. (bvsalud.org)
To gain insights into how targeting LSD1 led to the expansion of stem cells, the group performed gene expression profiling. (lu.se)

Collectively our results demonstrate that SKI is an important regulator of hematopoietic stem cell activity and its overexpression leads to myeloproliferative disease. (edu.au)

Anatomy54

Organisms14

Diseases25

Chemicals and Drugs59

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Psychiatry and Psychology1

Phenomena and Processes46

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Named Groups3

Health Care5

Transplantation18

Differentiation26

Progenitors9

HSCs27

Long-term hematopoietic stem1

Stromal cells2

Progenitor cell1

Pluripotent stem5

Cord5

Hematopoiesis7

Proliferation8

Engraftment of human1

Differentiate4

Receptor2

Mice7

Murine4

Lymphocytes3

Embryonic stem1

Vitro and in vivo2

CD344

Peripheral blood3

Autologous1

Assay1

Tissues5

Cytokines7

Proliferate1

20172

Lineages3

ABSTRACT1

Malignant1

Macrophages2

Self-renew4

Adult2

IPSC2

Clonal1

Extracellular2

Proteins2

Lineage2

Therapy10

Endothelial3

Tissue7

Lentiviral1

Fetal Blood1

Mobilization1

Precursors1

20162

Biology1

MSCs4

Genetic2

Citation1

Homeostasis1

Flow Cytometry2

Clinical trials2

Subramaniam1

Gene expression3

Regulator1