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  • HSCs
  • Subjecting transduced HSCs to an ex vivo expansion protocol following the transduction procedure could partially reverse this loss. (ox.ac.uk)
  • CONCLUSIONS: These studies suggest that loss of repopulating ability of transduced HSCs rather than low gene transfer efficiency might be the main problem in clinical gene therapy protocols, and that a clinically feasible ex vivo expansion approach post-transduction can markedly improve reconstitution with gene marked stem cells. (ox.ac.uk)
  • I then tested the therapeutic efficacy of ex vivo expanded HSCs in nonmyeloablated mice with severe β-thalassemia caused by the homozygous deletion of the β-major globin gene (β-MDD). (ubc.ca)
  • Together these data provide "proof of principle" of the curative potential of ex vivo expanded HSCs in a preclinical model of β-thalassemia treated with nonmyeloablative conditioning. (ubc.ca)
  • For example, knockdown of select polarity-associated genes leads to enhanced (Prox1) or decreased (Pard6a, Prkcz, and Msi2) repopulation potential of HSCs in vivo. (rupress.org)
  • Tregs
  • We previously reported that TNFRSF25 stimulation with an agonist Ab, 4C12, expands pre-existing CD4 + Foxp3 + regulatory T cells (Tregs) in vivo. (jimmunol.org)
  • TNFRSF25 stimulation by TL1A-Ig in vivo induced expansion of Tregs such that they increased to 30-35% of all CD4 + T cells in the peripheral blood within 5 d of treatment. (jimmunol.org)
  • TL1A-Ig-expanded Tregs expressed high levels of activation/memory markers KLRG1 and CD103 and were highly suppressive ex vivo. (jimmunol.org)
  • TL1A-Ig-mediated Treg expansion in vivo was protective against allergic lung inflammation, a mouse model for asthma, by reversing the ratio of conventional T cells to Tregs in the lung and blocking eosinophil exudation into the bronchoalveolar fluid. (jimmunol.org)
  • Thus, TL1A-Ig fusion proteins are highly active and tightly controllable agents to stimulate Treg proliferation in vivo, and they are uniquely able to maintain high levels of expanded Tregs by repeated administration. (jimmunol.org)
  • stem cell f
  • Publications] Ueda T.: 'Expansion of human NOD/SCID-repopulating cells by a combination of stem cell factor, Flk2/Flt3 ligand, thrombopoietin and a complex of interleukin-6 and soluble interleukin-6 receptor. (nii.ac.jp)
  • stimulation
  • Expansion was far retarded during third round stimulation. (jimmunol.org)
  • Pools of overlapping peptides from antigenic proteins for T-cell stimulation, T-cell expansion in T-cell assays such as ELISPOT, ICS or Flow Cytometry, e.g. for immune monitoring. (jpt.com)
  • vitro expansion
  • Upon in vitro expansion, primary EPC/ECFC were characterized by immunohistochemical analysis, showing a uniform positivity for the specific endothelial marker Von Willebrandt factor (Factor VIII), as well as for CD105 (Figure 3B) and CD(data not shown). (cathepsininhibitor.com)
  • amniotic membrane
  • Because the amniotic membrane contains a basement membrane resembling that from the cornea 4 and the conjunctiva 5 and such growth factors such as EGF, KGF, HGF, bFGF, 6 , 7 and NGF, 8 it has been considered an ideal matrix to support ex vivo expansion of LEPC. (arvojournals.org)
  • gene
  • Protransduzin™ the highly efficient transduction enhancer increases viral gene transfer in pre-clinical and clinical applications, such as ex vivo gene therapy and CAR-T cell development. (jpt.com)
  • SHEM
  • These data collectively suggest that MESCM can be used to replace SHEM to further promote expansion of LEPC by maintaining limbal native NCs. (arvojournals.org)
  • chemotherapy
  • Lymphodepleting patients with chemotherapy before T-cell transfer enhances in vivo T-cell expansion, but results in nonspecific destruction of the resident immune system and can have significant toxicity. (jpt.com)
  • therapeutic
  • Therefore, deciphering the molecular mechanisms that induce β-cell expansion can be of great value for therapeutic approaches aimed at increasing β-cell mass in diabetes. (diabetesjournals.org)
  • 6,7 However, the benefit of ex vivo-expanded EPCs to the damaged organs along with the number of EPCs recruited to the damaged tissues varies considerably in the previous studies, 8-10 suggesting that a better understanding of the mediators and receptors responsible for the process of EPC trafficking and recruitment would be crucial to enhancing EPC-mediated therapeutic effect. (ahajournals.org)
  • potential
  • Ex vivo expansion has the potential to provide a safe, effective means of increasing cell numbers. (edu.au)
  • This study has identified optimized fold expansion conditions, with the potential for direct clinical translation to increase transplantable cell dose and as a baseline methodology against which future factors can be tested. (edu.au)
  • In this thesis I have explored the potential of forced overexpression of homeobox genes as a strategy to obtain the degree of HSC expansion required. (ubc.ca)
  • self-renewal
  • With these conditions, serial expansion showed continual target population expansion and consistently higher expression levels of self-renewal associated genes. (edu.au)
  • fold
  • Maximal expansion of LTC-IC and CFU- GEMM were 2.58 and 2.37 fold respectively after 9 days of culture, and were obtained with the combination of IL-3 SCF FL TPO. (koreamed.org)
  • nT reg expansion was ~50 million-fold when flow sort-purified nT regs were restimulated four times with aAPCs. (sciencemag.org)
  • The expansion rate was estimated at 4.2-fold by a limiting dilution method. (nii.ac.jp)
  • effective
  • and (b) growing the VSELs in a culture medium that comprises a histone deacetylase (HDAC) inhibitor, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and optionally transforming growth factor beta (TGFβ), wherein the culture medium comprises an effective amount of the HDAC inhibitor, LH, FSH, and optionally TGFβ to result in expansion of the VSELs. (freepatentsonline.com)
  • function
  • 1 In animal models of myocardial infarction (MI), injection of ex vivo-expanded EPCs significantly improved blood flow and cardiac function and reduced left ventricular (LV) scarring. (ahajournals.org)
  • Authors
  • The authors found that in vivo insulin sensitivity was related to a higher in vitro capacity for fat oxidation. (jci.org)
  • blood
  • Since the hematopoiesis might be controlled by both positive and negative factors, the finding of negative cellular components and thereafter depletion of them would be of importance for further expansion of HPC from cord blood in vitro. (haematologica.org)
  • Activity
  • However, infused EBV-specific CTL did not expand in vivo, likely limiting their antitumor activity. (jpt.com)
  • However
  • however, the initial number of nT regs in UCB units is limited, and average yield after expansion was only 1 × 10 9 nT regs . (sciencemag.org)
  • However, an absence of consensus regarding optimum expansion conditions prevents standard implementation. (edu.au)
  • However, the effect of direct PKC-ζ activation in the β-cell in vivo is unknown. (diabetesjournals.org)