Vivo differentiation hematopoietic stem cells embryonic cell differentiation. Medical search. Frequent questions

Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
However, metabolic processes underlying the emergence of HSCs from HE cells remain unclear. (lu.se)
The major shortcoming in generating functional hematopoietic stem cells (HSC) from pluripotent stem cells is the lack of adequate markers for specification and maintenance of functional human HSCs. (ca.gov)
Using these reporter lines to monitor the induction of HSC "stemless' program, we are making progress in optimizing differentiation conditions toward generating functional HSCs for transplantation. (ca.gov)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
moreover, they also promote the hematopoietic differentiation of mouse ESCs and favor the development of definitive HSCs from pre-HSCs in the mouse aorta-gonad-mesonephros (AGM) region [ 8 ]. (biomedcentral.com)
Identification of genes involved in in vitro differentiation induction of embryonic stem cells (ESCs) into hematopoietic stem cells (HSCs) has been challenged during last decade. (ebiotrade.com)
During fetal development, expansion of HSCs (self-renewal) and production of differentiated haematopoietic cells (differentiation) are both required to sustain the haematopoietic system for body growth 3,4 . (elsevierpure.com)
Here we used in vivo genetic tracing in mice to analyse the formation of HSCs and progenitors from intra-arterial haematopoietic clusters, which contain HSC precursors and express the transcription factor hepatic leukaemia factor (HLF). (elsevierpure.com)
Through kinetic study, we observed the simultaneous formation of HSCs and defined progenitors-previously regarded as descendants of HSCs 5 -from the HLF + precursor population, followed by prompt formation of the hierarchical haematopoietic population structure in the fetal liver in an HSC-independent manner. (elsevierpure.com)
The transcription factor EVI1 is heterogeneously expressed within the precursor population, with EVI1 hi cells being predominantly localized to intra-embryonic arteries and preferentially giving rise to HSCs. (elsevierpure.com)
These data suggest that fetal HSCs minimally contribute to the generation of progenitors and functional blood cells before birth. (elsevierpure.com)
Although the use of hematopoietic stem cells (HSCs) for therapeutic purposes has been widely applied in clinic 11-13 , HSCs have reduced differentiation and proliferative capacities, and HSCs are difficult to expand in in vitro cell culture 14-16 . (psu.edu)
Thus, iPS cells have greater potential to be used in ACT-based immunotherapy compared to ESCs or HSCs. (psu.edu)
HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
Hematopoietic stem cells (HSCs), first produced in the developing vertebrate embryo, supply the lifelong foundation of the blood and immune systems. (grantome.com)
Likewise, while the number of transplanted HSCs is well known to directly impact engraftment efficiency, there are currently no established clinical protocols to successfully expand donor-harvested HSCs, nor to differentiate embryonic or induced pluripotent stem cells (iPSCs) into functional HSCs in vitro. (grantome.com)
Therefore, the identification of novel modifiers of de novo production of HSCs with long-term self-renewal and differentiation capacity is a major unmet clinical need. (grantome.com)
In the developing embryo, definitive HSCs arise from a unique population of mesodermal precursors termed hemogenic endothelium (HEC) through a process known as endothelial-to-hematopoietic transition (EHT). (grantome.com)
Defining the molecular signaling pathways that mediate productive HSC formation in vivo will reveal new targets for optimizing the directed expansion and/or differentiation of adult-type HSCs for clinical use. (grantome.com)
Hematopoietic stem cells (HSCs), produced in the developing embryo, form the foundation of our blood system. (grantome.com)
Haematopoietic stem cells (HSCs) have been used in the clinic for several decades. (ox.ac.uk)
Yet, our capacity to grow, expand and manipulate HSCs is still limited, which is holding back a more wider application of HSC-based therapies, for example in therapies aimed at correcting single gene haematopoietic disorders by genome editing. (ox.ac.uk)
The Developmental Haematopoiesis Group aims to learn from the embryo how HSCs are specified and how their self-renewal and differentiation into functional end cells is controlled, and apply this knowledge for the purpose of regenerative medicine. (ox.ac.uk)
Making use of this reporter in in vivo HSC development and ESC/iPSC differentiation cultures, our goal is to further the understanding of how HSCs are specified and born. (ox.ac.uk)
Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)

The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
During embryonic development, hematopoiesis occurs through primitive and definitive waves, giving rise to distinct blood lineages. (lu.se)
Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
Expression trapping: identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells. (ox.ac.uk)
These neural precursor cells can be easily harvested from a minimally invasive nasal biopsy, making them a valuable cell source to study human neural cell lineages in health and disease. (gatech.edu)
Especially Mesenchymal Stem cells have been focused in a plethora of preclinical and clinical experiments over the five decades due to extraordinary capacity in differentiation to multiple lineages, immunomodulation and angigenic properties. (samploon.com)
Large body of data of experiment concludes that EMSCs have the ability to differentiation in multiple lineages such as osteoblast, cartilage, adipose, smooth muscle cell(1/18), hepatocytes, cardiomyocyte, neural cells. (samploon.com)
These stem cells are considered committed to certain cell lineages. (biomedcentral.com)
Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
Similarly, the cells showed differentiation ability into all three lineages of cells tested. (bvsalud.org)

We demonstrate that glycolysis-mediated differentiation of HE toward primitive erythroid hematopoiesis is dependent on the epigenetic regulator LSD1. (lu.se)
In contrast, OXPHOS-mediated differentiation of HE toward definitive hematopoiesis is dependent on cholesterol metabolism. (lu.se)
Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
The inventors have developed a novel system for co-differentiation of hepatocytes and hematopoietic progenitors from human embryonic stem cells (ES cells) that mimics a late fetal liver undergoing hematopoiesis. (warf.org)
We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

During embryonic development mesodermal differentiate into endothelial cells, hemangioblast and hematopoietic progenitors give rise to blood vessel, the vasculogenesis. (samploon.com)

Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
Previous studies have proven that rodent and human MSCs can differentiate into HLCs in vitro and in vivo , which is one of the mechanisms of MSCs in the treatment of liver failure. (springer.com)
In addition, like ESCs, iPS cells possess indefinite proliferative capacity in vitro, and have been shown to differentiate into hematopoietic cells. (psu.edu)
The pluripotent stem cells differentiate further into cells commonly called multipotent stem cells. (biomedcentral.com)
Until recently it was considered a fact that, for example, hematopoietic stem cells did not have the potential to differentiate into cells of other types than the cells constituting the blood system. (biomedcentral.com)
They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)

As of 2016[update], the only established therapy using stem cells is hematopoietic stem cell transplantation. (wikipedia.org)
This usually takes the form of a bone-marrow transplantation, but the cells can also be derived from umbilical cord blood. (wikipedia.org)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
Furthermore, transplantation of Lhx2-transduced hematopoietic cells into lethally irradiated mice resulted in multi-lineage repopulation of hematopoietic cells over 4 months. (ebiotrade.com)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
in 1956 and Till and McCulloch in 1961, demonstrating that lethally irradiated mice could be rescued with cells from freshly isolated bone marrow [ 10 ] and that BM cells formed spleen clonies (CFU-S) following transplantation to irradiated recipients [ 30 ], respectively. (biomedcentral.com)
For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)

These lines will serve as tools to identify factor combinations to improve the in vitro differentiation of hESCs to functional blood stem cells. (ca.gov)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
These observations imply that current in vitro differentiation strategies are missing critical cues which are essential to unlock or maintain full HSC potential in vivo. (grantome.com)

Preliminary studies related to multiple sclerosis have been conducted, and a 2020 phase 2 trial found significantly improved outcomes for mesenchymal stem cell treated patients compared to those receiving a sham treatment. (wikipedia.org)
Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro , and cell deployment in vitro and in vivo , can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. (nature.com)
Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)

However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
Our studies also suggest that these hESC reporter lines will become valuable for investigators working on optimizing stem cell differentiation and culture for other tissues, as many of these reporter genes are not only restricted to HSC. (ca.gov)
however, regulations for treating patients with cells and tissues that are based on evidence could help to address challenges relating to therapy, safety, and efficacy. (nationalacademies.org)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
Stem cell-independent pathways during development thus offer a rational strategy for the rapid and simultaneous growth of tissues and stem cell pools. (elsevierpure.com)
Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
During the development of embryonic and adult tissues, there is stage-specific, sequential expression of cytokine receptors during differentiation. (gatech.edu)
A few of the cells in the inner cell mass will develop into the foetus, whereas the rest will form the placenta and other supporting tissues needed for foetal development in the uterus. (biomedcentral.com)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Over the last years, dentistry has been exploring the potential application of stem cells from different origins in the regeneration of oral tissues that were lost or damaged by disease or trauma 1 . (bvsalud.org)

We generated an Aplnr-tdTomato reporter mouse embryonic stem cell (mESC) line and showed that HSPCs are generated exclusively from mesodermal cells that express Aplnr. (ed.ac.uk)
The objective of this 2-year project is to take the first step toward applying our bioreactor technology to ES cell research, by expanding and maintaining undifferentiated mouse embryonic stem (mES) cells in laboratory-scale versions of our bioreactor. (grantome.com)
Mouse embryonic fibroblasts isolated from transgenic Oct4-GFP reporter mice with or without Sirt6 were used for reprogramming by Yamanaka factors. (biomedcentral.com)

Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
Heterochromatin exists in small regions (nucleation sites) in stem cells (for example, embryonic or hematopoietic stem cells) that expand differentially depending on the lineage track. (ucsf.edu)
Transcriptional activity is regulated by DNA binding transcription factors, but these typically act in more than one cell lineage activating different subsets of target genes in each. (edu.au)
Their cell lineage-specific effects are governed by chromatin structure. (edu.au)
To further understand the epigenetic regulators for specific lineage differentiation from iPS cell would have great significance for potential regeneration therapy and human disease modeling [ 8 ]. (biomedcentral.com)

Compounds of the trifluoromethyloxadiazole and hydroxamic acid series exhibited promising anti-proliferative activities, HDAC6 targeting in PCA cells, and in vitro tumor selectivity. (bvsalud.org)
CTLs can recognize malignant cells by interacting tumor antigens with the T cell receptors (TCR), and release cytotoxins as well as cytokines to kill malignant cells. (psu.edu)
Stimulation in vitro with a Notch ligand drives T cell differentiation from iPS cells, and TCR gene transduction results in iPS cells differentiating into antigen-specific T cells in vivo, which prevents tumor growth. (psu.edu)
Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
Sirt1, the mouse homologue of yeast Sir2 deacetylates several non-histone proteins and plays roles in many key functions, including energy metabolism, differentiation, aging, and tumor suppression [ 10 ]. (biomedcentral.com)
Cells were in that case incubated with 100 L of moderate per good containing 20 L from the CellTiter 96 Aqueous A single Option reagent for 2 h in 37 C. cell apoptosis while suppressed cell development, cell invasion and migration and xenograft tumor development in vivo. (woofahs.com)

Next, I will describe some work on the differentiation of neural precursor cells isolated from human olfactory epithelium (OE). (gatech.edu)
The results indicated that GSK-3 inhibition promotes the early stages of neuronal differentiation and decrease proliferation in cultures of adult human neural precursors. (gatech.edu)
We show that the conditional depletion of the m 6 A reader protein Ythdf2 in mice causes lethality at late embryonic developmental stages, with embryos characterized by compromised neural development. (biomedcentral.com)
We demonstrate that neural stem/progenitor cell (NSPC) self-renewal and spatiotemporal generation of neurons and other cell types are severely impacted by the loss of Ythdf2 in embryonic neocortex. (biomedcentral.com)
We have shown that MOZ/MYST3 is essential for the development of haematopoietic stem cells and that QKF/MYST4 has a critical role in neural stem cells and in brain development. (edu.au)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

Activation of the Aplnr pathway in this system impaired the generation of long-term reconstituting HSPCs and appeared to drive myeloid differentiation. (ed.ac.uk)
These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
Committed myeloid progenitor cells and cells with long term reconstituting potential were lost from bone marrow within four days after poly injection. (biomedcentral.com)

Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
However, due to difficulties in obtaining a high number of such CTLs from patients, there is an urgent need to find a new approach to generate highly reactive Ag-specific CTLs for successful ACT-based therapies.TCR transduction of the self-renewable stem cells for immune reconstitution has a therapeutic potential for the treatment of diseases 8-10 . (psu.edu)
Here, we present methods for the generation of T lymphocytes from iPS cells in vitro, and in vivo programming of antigen-specific CTLs from iPS cells for promoting cancer immune surveillance. (psu.edu)
In vivo experiment performed without occurrence of immune rejection. (samploon.com)

Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Combining in vivo and in vitro assays, we show that the proliferation and differentiation capabilities of NSPCs decrease significantly in Ythdf2 −/− embryos. (biomedcentral.com)

The Japanese government has made innovation, including innovation in stem cell research, a national priority, and new laws regarding regenerative medicine products are expected to provide a more efficient path to the translation of these therapies to the clinic. (nationalacademies.org)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
Controlling the expansion and differentiation of stem and progenitor cells in vivo and in vitro is an important goal in regenerative medicine and for the development of patient-based, disease-relevant cellular models. (gatech.edu)
This regenerative capacity is due to presence of EnSCs that were demonstrated to be immuno-privileged in comparison with other cell types, shedding a new light for cell-based therapies and rendering these cells as a promising resource in regenerative medicine. (samploon.com)
During menstruation, all these cells could be found as menstrual stem cells or endometrial regenerative cells (ERC). (samploon.com)
It includes the possible stem cell therapy mechanisms involved and outcomes recorded so far, the limitations of using these regenerative medicines, and the progressive improvement in stem cell therapy by adopting approaches like PiggyBac, Sleeping Beauty, and the Sendai virus. (biomedcentral.com)
Microglial phagocytosis of apoptotic cells (efferocytosis) is at the core of the brain regenerative response. (frontiersin.org)
They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)

The Cell Manipulations Laboratory's experienced scientists can help you develop and scale up your ex-vivo gene transfer and cell therapy clinical trials or simply transfer your existing at-scale technology to our clean facilities which will enable you to move your research to a safe and effective patient treatment plan. (cincinnatichildrens.org)
Researchers and clinicians can contact us for more information about how we can help you with your cell therapy and gene transfer needs. (cincinnatichildrens.org)
Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
Recent iPS cell technology and the development of an in vitro system for gene delivery are capable of generating iPS cells from patients without any surgical approach. (psu.edu)
This study aims to characterize the role of biomechanical modulation of the hemogenic vascular niche in HSC formation in vivo and in vitro, with the overall goal of identifying the signaling pathway(s) connecting select biophysical forces to the gene regulatory network controlling HSC commitment. (grantome.com)
We have developed a large-scale, expression-based gene trap strategy to perform genome-wide functional analysis of the murine hematopoietic and vascular systems. (ox.ac.uk)
Using two different gene trap vectors, we have isolated embryonic stem (ES) cell clones containing lacZ reporter gene insertions in genes expressed in blood island and vascular cells, muscle, stromal cells, and unknown cell types. (ox.ac.uk)
The diverse cell types are genetically identical, but differ in their form and function due to differences in gene expression. (edu.au)
Therefore, gene activity is an important mechanism in embryonic development and adult stem cell differentiation. (edu.au)

Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)

a donor's healthy bone marrow reintroduces functional stem cells to replace the cells lost in the host's body during treatment. (wikipedia.org)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
Information exchange between cells coordinates devel- opment and functional interplay in complex organisms. (unicyte.ch)

In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
Expression and sequence analysis of representative clones suggest that this approach will be useful for identifying and mutating novel genes expressed in the developing hematopoietic and vascular systems. (ox.ac.uk)
Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
RNA sequencing was measured to identify the differential expressed genes due to loss of Sirt6 in somatic and pluripotent cells. (biomedcentral.com)
Despite significant advances in understanding nephron segment patterning, many questions remain about the underlying genes and signaling pathways that orchestrate renal progenitor cell fate choices and regulate differentiation. (mdpi.com)

By genetically manipulating EVI1 expression, we were able to alter HSC and progenitor output from precursors in vivo. (elsevierpure.com)
The differentiated hematopoietic precursors express mature markers that show promise for engraftment (study in progress). (warf.org)
and Tracing the origin of HE back to gastrulation to begin to map the factors acting on the precursors of these cells. (ox.ac.uk)

Our goal is to generate a toolbox of human embryonic stem cell lines to monitor blood forming (hematopoietic) stem cell development in culture. (ca.gov)
To help overcome these bottlenecks, we will generate a suite of human embryonic stem cell reporter lines that can be used to monitor key milestones in blood stem cell development. (ca.gov)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

Runx1 is critically required along the transition of HE to definitive haematopoietic stem and progenitor cells, and several of its known transcriptional targets act in this transition. (ox.ac.uk)
Particularly, by performing transcriptome analysis, we observed that several pluripotent transcriptional factors increase in knockout cell line, which explains the underlying loss of pluripotency in Sirt6-null iPS-like cell line. (biomedcentral.com)
Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)

It is known that less-differentiated and central-memory-like (termed highly reactive) CTLs are the optimal population for ACT-based immunotherapy, because these CTLs have a high proliferative potential, are less prone to apoptosis than more differentiated cells and have a higher ability to respond to homeostatic cytokines 2-7 . (psu.edu)
This usually achieved by exposing stem and progenitor cells to specific types and doses of cytokines, growth factors and extracellular matrix proteins. (gatech.edu)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)

Here, we show that HSC-like cells were efficiently induced from mouse ESCs by enforced expression of Lhx2, a LIM-homeobox transcription factor. (ebiotrade.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
However, the approach to obtain embryonic stem cells (ESCs) from patients is not feasible. (psu.edu)

Thus, it is necessary to develop culture systems which produce pure populations of tissue-specific stem-cells in vitro without the loss of stem-cell potential. (wikipedia.org)
The clinical application of MSCs to tissue engineering has been introduced due to their numerous advantages including high expansion potential and extensive differentiation potential [ 6 , 7 ]. (hindawi.com)
CML offers more-than-minimally manipulated cell and tissue procedures performed aseptically in our cleanroom in accordance with GMP / GTP quality compliance requirements . (cincinnatichildrens.org)
In the United States, stem cell therapies are regulated as biologics and are subject to premarket approval under the risk-based approach to approving cellular and tissue-based products. (nationalacademies.org)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
and 2) bioreactor-based directed differentiation of ES cells under perfused 3D tissue-density conditions. (grantome.com)
Tissue regeneration approach using stem cells in different clinical settings is a medical sensation now-a-days. (samploon.com)
Human endometrial tissue composed of epithelial stem cells (0.22%), mesenchymal stem cells (1.25%) and side population stem cells. (samploon.com)
EMSCs also have the high capacity proliferation, multidirectional differentiation that is indicative for the potentiality for tissue regeneration. (samploon.com)
However, they cannot form an organism because they are unable to give rise to extra embryonic tissue essential for normal development in the uterus. (biomedcentral.com)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair. (scirp.org)
The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
Materials and method: The pulp tissue, obtained from wisdom teeth, was placed in a 6-well plate containing proper culture medium, and stored at 37 °C and 5% CO2 for cell proliferation and plastic adherence. (bvsalud.org)
The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)

Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
Small hepatic progenitor cells (SHPCs) derived from these hepatocytes have shown mature metabolic functions as well as engraftment in immunodeficient mice. (warf.org)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
In mice, homozygous loss of Runx1 results in embryonic lethality and a complete absence of definitive haematopoietic cells. (ox.ac.uk)
To study the role of THOC5/Fms interacting protein in vivo , we generated THOC5/Fms interacting protein knockout mice. (biomedcentral.com)
Since these mice are embryonic lethal, we then generated interferon inducible conditional THOC5/Fms interacting protein knockout mice. (biomedcentral.com)
Furthermore, infusion of normal bone marrow cells rescued mice from death induced by loss of THOC5/Fms interacting protein. (biomedcentral.com)
Furthermore, the embryonic development of the conventional THOC1 knockout mice is arrested around the time of implantation [ 13 ], suggesting that the THO complex may play an essential role in early development. (biomedcentral.com)

These agents, however, cannot discriminate between the leukaemia or neoplastic cells, and the hematopoietic stem cells within the bone marrow. (wikipedia.org)
It is an allogenic stem therapy based on mesenchymal stem cells (MSCs) derived from the bone marrow of adult donors. (wikipedia.org)
Self-renewal and differentiation are tightly controlled to maintain haematopoietic stem cell (HSC) homeostasis in the adult bone marrow 1,2 . (elsevierpure.com)
The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells. (nature.com)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
We used adult bone marrow erythropoiesis (red blood cell development) as a model system since it depends on the sequential action of stem cell factor (SCF), an early-acting cytokine and erythropoietin (EPO), a late-acting cytokine. (gatech.edu)
Investigation of bone marrow cells showed that these became apoptotic within seven days after poly injection. (biomedcentral.com)

Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
Khetan, S. & Burdick, J. A. Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels. (nature.com)
The proposed research will elucidate how biomechanical forces resulting from the onset of embryonic circulation cue the cellular commitment and vascular remodeling necessary for HSC formation via Rho-Yap activation. (grantome.com)
It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. (bdbiosciences.com)
Additionally, the interwoven fibers provide a scaffold allowing the cells to form 3D structures where the size of cellular aggregates is limited by the spacing between the hollow fibers. (grantome.com)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
During embryonic development, the unique cellular phenotypes of the adult are established. (edu.au)
not unique for the embryonic system but are shared by other ogenesis regulation must consider different scales of multi-cellular modeling areas. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)

Leukemia inhibitory factor (LIF), Hepatocyte growth factor (HGF) and stem-cell factor (SCF) have the essential contribution in endometrial regeneration. (samploon.com)
Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)

As a result, human cells, with the exception of those deemed minimally manipulated and used for autologous treatments, would be subject to FDA premarket approval as biologics. (nationalacademies.org)
Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)

In addition to the functions of the cells themselves, paracrine soluble factors produced by stem cells, known as the stem cell secretome, have been found to be another mechanism by which stem cell-based therapies mediate their effects in degenerative, autoimmune, and inflammatory diseases. (wikipedia.org)
These tools will help translate the promise of stem cells to cell based therapies to treat human disease. (ca.gov)
Stem cell therapies are regulated differently in various countries around the world, with some countries offering stem cell therapies that are not available elsewhere. (nationalacademies.org)
While stem cell therapies offered in China must be registered, there are not yet approved quality control guidelines available. (nationalacademies.org)
If regulations for stem cell therapies were only just now being developed in the United States, they would look much different than the ones that exist today, said Margaret Foster Riley, professor of law at the University of Virginia School of Law. (nationalacademies.org)
The effectiveness of stem cell therapies has been hampered by cell death and limited control over fate. (nature.com)
Embryonic stem (ES) cell research and scale-up for development of possible clinical therapies is limited by the existing 2D dish culture methods. (grantome.com)

Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
While mechanical properties of the niche, including sheer stress and circumferential stretch, are increasingly recognized as important stem cell cues in many contexts, the mechanism(s) by which mechanotransduction drives commitment to hemogenic fate and HSC productionduring vertebrate development remain largely unexplored. (grantome.com)
There are different types of stem cells that best can be described in the context of normal human development [ 23 ]. (biomedcentral.com)
Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
We are currently investigating the function of chromatin modifiers during embryonic development, in adult stem cell populations and in cancer, and modelling chromatin-based intellectual disability to discover ameliorating treatments. (edu.au)
A tight spatial and timing regulation of growth factor action during embryonic development has been suggested [4]. (unicyte.ch)
From development to aging and disease, the brain parenchyma is under the constant threat of debris accumulation, in the form of dead cells and protein aggregates. (frontiersin.org)

In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. (scirp.org)
These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. (scirp.org)

Each of those cell sources has specific advantages and disadvantages, and each has found particular clinical applications. (medscape.com)

1) Hepatocytes generated in this system display late fetal markers (both albumin and alpha-fetoprotein, AFP) and give rise to mature and engraftable small hepatic progenitor cells (SHPCs, which express albumin but not AFP). (warf.org)

In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
Adoptive cell transfer (ACT) of antigen-specific CD8 + cytotoxic T lymphocytes (CTLs) is a promising treatment for a variety of malignancies 1 . (psu.edu)

Transduction of Lhx2 into induced pluripotent stem cells (iPSCs) was also effective in generating KSL cells in vitro, as well as HSC-like activities in vivo. (ebiotrade.com)
The different investigations described above both required the use of tools to examine how signal transduction operates in single live cells. (gatech.edu)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)

Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
Apelin receptor (Aplnr/Agtrl1/Apj) marks a transient cell population during the differentiation of hematopoietic stem and progenitor cells (HSPCs) from pluripotent stem cells but its function during the production and maintenance of haematopoietic stem cells is not clear. (ed.ac.uk)

In this seminar, I will first describe our investigation of the mechanisms responsible for the combinatorial cytokine dependence of stem/progenitor cell fate decisions. (gatech.edu)

A fertilized egg is totipotent, meaning that its potential is total, and as the fertilized egg divides all the cells in the embryo remain totipotent until the fertilized egg has reached the 8/16-cell stage. (biomedcentral.com)
In theory, and to some extent in practice, an 8-cell embryo can be divided into eight single cells, and each cell has the potential of generating an individual if implanted in a woman's uterus. (biomedcentral.com)

In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate. (nature.com)
Heterochromatin spreading plays a key role in the genome partitioning process that shapes cell fate. (ucsf.edu)
We hypothesized that combinatorial cytokine dependence arises in cell subsets in unique transitional stages where cell fate decision can be regulated by combinations of early- and late-acting factors. (gatech.edu)
We have identified a subset of hematopoietic progenitor cells which fate decisions depend on both the SCF and EPO signals. (gatech.edu)
In these experiments, ATP-competitive (6-bromoindirubin-3'-oxime and CHIR99021) and substrate-competitive (TAT-eIF2B) inhibitors were used in order to eliminate potential confounding effects on cell fate due to off-target kinase inhibition. (gatech.edu)
Therefore, in the last part of the seminar, I will explain how single-cell CE and mass spectrometry (MS) can be combined to examine the fate of enzyme activity reporters and inhibitors in single mammalian cells with a resolution at the single amino acid level. (gatech.edu)
In normal cells, the fate of pyruvate depends on many factors, one of which is oxygen availability. (hindawi.com)
The fate of the cell is deter- mined by coordinated and dynamic interactions among a number of factors, acting in a defined microenviron- ment. (unicyte.ch)

Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. (ox.ac.uk)

To track the location and quantity of transplanted cells in recipient small animal model, we will generate hESC lines with in vivo reporter system that combines bioluminescent or PET imaging, and serum-based assay. (ca.gov)
To facilitate the identification and isolation of HE and cells undergoing EHT for mechanistic studies, we generated a Runx1 +23 enhancer-reporter model (23GFP). (ox.ac.uk)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

We study the embryonic origins of blood stem cells with the aim to inform the generation of these cells in culture, and ultimate produce clinically relevant blood stem cells for therapeutic purposes. (ox.ac.uk)

Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

Stem-cell therapy is the use of stem cells to treat or prevent a disease or condition. (wikipedia.org)
Stem-cell therapy has become controversial following developments such as the ability of scientists to isolate and culture embryonic stem cells, to create stem cells using somatic cell nuclear transfer and their use of techniques to create induced pluripotent stem cells. (wikipedia.org)
this is the only widely practiced form of stem-cell therapy. (wikipedia.org)
Another stem-cell therapy, called Prococvhymal, was conditionally approved in Canada in 2012 for the management of acute graft-vs-host disease in children who are unresponsive to steroids. (wikipedia.org)
In some cases, offering a particular stem cell therapy can have a positive economic impact for the country in which it is offered because patients are willing to travel in hopes of finding effective therapeutic options. (nationalacademies.org)
Wollert, K. C. & Drexler, H. Cell therapy for the treatment of coronary heart disease: A critical appraisal. (nature.com)
A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)

The insights obtained from these studies will inform protocols to generate definitive HE and blood cells from ESC/iPSC. (ox.ac.uk)

Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
This can also be achieved by directly modulating the activity of signaling enzymes or transcription factors using genetic engineering strategies or by delivering bioactive molecules in the cell cytosol or nucleus. (gatech.edu)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)

Once individual lines have been validated, lines that contain multiple fluorescent reporters will be generated, and a multi factor screen will be performed to optimize conditions that induce these blood stem cell regulators. (ca.gov)
Different groups have established several protocols to induce the differentiation of MSCs into HLCs in vitro . (springer.com)
Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)

The majority of ES clones that expressed lacZ in blood islands also expressed lacZ upon differentiation into hematopoietic cells on OP9 stromal layers. (ox.ac.uk)
Since endometrial stromal cells are easy to isolate, and expand rapidly from patients without leading to major ethical and technical problems. (scirp.org)

Examples of multipotent stem cells are those producing blood, nerve and muscle cells. (biomedcentral.com)
Multipotent stem cells are present in the adult individual as well as the fetus and umbilical cord. (biomedcentral.com)
The established dogma has however been challenged by recent findings suggesting that multipotent stem cells have a broader differentiation potential than previously thought. (biomedcentral.com)
Although several reports indicate an ability of multipotent stem cells to produce cells even outside their normal limits, most of these findings remain highly controversial. (biomedcentral.com)

Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
MSCs naturally reside in a specialized niche in vivo , which mainly consists of the extracellular matrix (ECM). (hindawi.com)
13) Like other sources, endometrial MSCs have recently been emerged as a promising alternative source of therapeutic MSCs due to its unique ability to undergo smooth muscle differentiation, migratory, angiogenic. (samploon.com)

In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
Then, we will discuss several principles that govern macrophage phagocytosis of apoptotic cells (efferocytosis), including the existence of redundant recognition mechanisms ("find-me" and "eat-me") that lead to a tight coupling between apoptosis and phagocytosis. (frontiersin.org)

Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
We mostly used multicolor flow cytometry in these studies but we have also explored several applications for single-cell capillary electrophoresis (CE). (gatech.edu)
Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)
Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
Mouse somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. (biomedcentral.com)
Differentiated somatic cells can be reprogrammed into a pluripotent-like state through four defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc (OSKM) [ 1 , 2 ]. (biomedcentral.com)
In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
Similarly, reprogramming of somatic cells involves a complex interaction among intracellular and extracellular signals leading to epigenetic remodeling [6]. (unicyte.ch)

Thus, we demonstrate antigen-specific T cell differentiation from iPS cells. (psu.edu)

Despite more than a decade of research, current protocols rely primarily on enforced expression of transcription factors to help steer cells into an ?HSC-like? (grantome.com)
Ident- cult to deduce interaction rules from observations of the ified transcription factors for specific cell fates are, for entire system. (lu.se)

Anatomy60

Organisms16

Diseases14

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment42

Phenomena and Processes58

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science3

Named Groups1

Health Care1

HSCs31

Lineages11

Hematopoiesis6

Progenitors1

Differentiate6

Transplantation11

Vitro differentiation3

Mesenchymal stem3

Tissues10

Mouse embryonic3

Lineage7

Tumor8

Neural7

Myeloid5

Neuronal differentiation1

Immune4

Proliferation and differentiation3

Regenerative9

Gene9

Adult stem5

Functional5

Genes5

Precursors3

Human embryonic3

Transcriptional3

Cytokines3

ESCs3

Tissue23

Mice8

Bone7

Cellular15

Regeneration3

Autologous2

Therapies7

Development7

Endometrial Stem Cells2

Clinical1

Hepatic progenitor cells1

Lymphocytes2

Transduction3

HSPCs3

Progenitor cell fate1

Embryo2

Fate9

Endothelium1

Reporter3

Blood stem cells1

Populations1

Therapy8

Generate1

Genetic3

Induce3

Stromal2

Multipotent4

MSCs3

Apoptosis2

Flow cytometry5

Somatic cells6

Antigen-specific1

Transcription2