• Functionally, cDC1 cross-present exogenous antigens to activate CD8 + T cells and can promote IL-12 dependent Th1 responses ( 1 , 5 - 7 ). (frontiersin.org)
  • Cross presentation is also required for the induction of cytotoxic immunity by vaccination with protein antigens, for example, tumour vaccination. (wikipedia.org)
  • pDCs are found within the blood and are able to cross present antigens directly or from neighboring apoptotic cells, but the main physiological significance of pDCs is the secretion of type I IFN in response to bacterial infections. (wikipedia.org)
  • All mDCs have specialized functions and secretory factors, but they are all still able to cross present antigens in order to activate cytotoxic CD8+ T cells. (wikipedia.org)
  • As a consequence, external soluble antigens are targeted into the MHC class I cross-presentation pathway instead of the MHC Class II pathway. (wikipedia.org)
  • However, knowledge about the biological properties of target antigens or modalities of antigen delivery to efficiently induce or expand MVA-vaccine-mediated immunity in vivo is still sparse. (uniklinik-duesseldorf.de)
  • Vaccines based on conserved antigens would not did not cross-react with M2e peptides from H5 and H7 sub- require prediction of which strains would circulate during type avian viruses that have 3 or 4 mismatches ( 6 ). (cdc.gov)
  • Introduction Antigens captured from the extracellular environment by APCs are processed and then presented on MHC class I molecules to CD8+ CTLs in a process called cross-presentation, resulting in the stimulation of CTLs, or cross-priming (1). (technuc.com)
  • Glycoprotein 96 (gp96) is a well-known HSP that promotes the cross-presentation of tumor antigens. (celljournal.org)
  • Our previous research has demonstrated that cross-presentation efficiency significantly increased when antigens are conjugated covalently to α-Al2O3 nanoparticles. (providence.org)
  • Antigens targeted to DCs via the C-type lectin Langerin/CD207 are cross-presented to CD8(+) T cells in vivo. (cnrs.fr)
  • in both cases, cross-presentation is required to generate CTLs that are specific for the causative infectious agents and tumor Ags (2). (technuc.com)
  • 4168-17-6 IC50 The current research analyzed whether 3D8 scFv was capable to gain access to the cross-presentation path in murine DCs and cross-prime CTLs. (technuc.com)
  • Therefore, it might be likely that stimulation of SR-A on DCs by fucoidan results in the crosspriming of OVA-specific CTLs. (bcrablinhibitor.com)
  • D) On day 35, in vivo killing of adoptively transferred SIINFEK-coated and CFSE-labeled target cells by CTLs in the immunized mice was measured. (bcrablinhibitor.com)
  • it cross-primed CTLs in HLA-A2 Tgm. (elsevierpure.com)
  • Unlike TFH and B cells that were potently activated in the steady-state, cross-priming of cytotoxic lymphocytes (CTLs) by Clec9A-targeted antigen required co-administration of adjuvant. (edu.au)
  • This vaccination induced challenge infection, DNA vaccination with NP and M antibodies that cross-reacted with divergent M2 peptide achieved limited protection against an H5N1 virus strain from an H5N1 subtype. (cdc.gov)
  • This M2 prime-boost vaccination conferred broad protection against challenge with lethal vaccine target. (cdc.gov)
  • OP9-DL1-induced cDC1s showed preferential migration toward CCR7 ligands in vitro and superior T cell cross-priming and antitumor vaccination in vivo. (mssm.edu)
  • Ex vivo IFN-γ T cell response to vaccination. (jci.org)
  • Vaccines generated by us are being studied in vitro and in vivo in preclinical animal models as well as in collaborative clinical studies. (uniklinik-duesseldorf.de)
  • SIRPα-Fc-CD40L activated a type I interferon response in macrophages and potentiated the activity of ADCP-competent targeted antibodies both in vitro and in vivo . (aacrjournals.org)
  • These cell cultures may provide a valuable in vitro and in vivo model to investigate mesothelioma biology. (biomedcentral.com)
  • Collectively, our findings establish new avenues for regenerative medicine, patient-specific iPS cell disease modelling and the study of early human development in vitro and in vivo. (nih.gov)
  • The co-stimulatory molecule CD40/CD40L along with the danger presence of an exogenous antigen are catalysts for dendritic cell licensing, and thus the cross presentation and activation of naive CD8+ cytotoxic T cells. (wikipedia.org)
  • Classical aluminum adjuvant is a deficient antigen carrier for cross-presentation and cross-priming of CD8+ cytotoxic T cells. (providence.org)
  • However, the protective capacity of cytotoxic T cells induced by conventional proteins has not been tested in vivo so far. (ox.ac.uk)
  • Therefore, one focus of our work is the identification of key mechanisms essential for the efficient generation (priming) and expansion (recall) of anti-viral or vaccine-induced T cell responses. (uniklinik-duesseldorf.de)
  • The HM48-1 mAb blocks binding of soluble CD2 to CD48-bearing cells, blocks the interaction of CD2 and CD244, inhibits spleen cell proliferative responses to mitogens, augments the proliferative response of spleen cells when cross-linked with anti-CD3e mAbs, and inhibits priming of CTL in vitro. (bdbiosciences.com)
  • The M2 (M2-Ad) had enhanced antibody responses that cross- ability of anti-M2 monoclonal antibody (MAb) to reduce reacted with human and avian M2 sequences and pro- viral replication ( 5 ) implicates M2, in particular M2e, as a duced T-cell responses. (cdc.gov)
  • Cross-presentation is important for initiating CTL responses against tumors. (technuc.com)
  • Molecules capable of transferring exogenous Ag to the cross-presentation pathway have been examined in a number of studies to better understand the mechanisms underlying cross-presentation and to develop tumor vaccines that enhance CTL responses. (technuc.com)
  • Fucoidan stimulates macrophage and DC activation by means of scavenger receptor-A (SR-A) in in vitro research [19,23,32], and it truly is likely that fucoidan may possibly stimulate in vivo spleen cDCs by engaging SR-A. Activation of SR-A benefits in human peripheral blood DC (PBDC) maturation that subsequently promotes Th1 responses [23]. (bcrablinhibitor.com)
  • DCs are identified to prime CTL responses upon activation by ligands targeting several PRRs, including toll-like receptors and Dectin-1 [33,34]. (bcrablinhibitor.com)
  • Our futurestudies will straight test regardless of whether fucoidan can activate SR-A and whether activation of SR-A signaling in DCs can promote CLR responses in vivo by utilizing SR-A-knock out mouse. (bcrablinhibitor.com)
  • A ) Median ex vivo IFN-γ enzyme-linked immunospot (ELISPOT) responses in peripheral blood mononuclear cells (PBMC) to the RH5 insert (summed response across all the individual peptide pools) shown for all groups. (jci.org)
  • Targeted delivery of antigen to CD8α+ DCs via Clec9A in vivo induces versatile immune responses, most notably potent thymus-dependent humoral responses even in the absence of adjuvant. (edu.au)
  • Furthermore, targeting Clec9A primed long-lived memory CD4+ T cells capable of robust secondary TFH responses, even in the absence of adjuvant. (edu.au)
  • This event leads to priming of T-cell responses. (fakpathway.com)
  • Although the function of medullary macrophages in T cell immunity has been unexplored so far, these macrophages effectively cross-primed the vaccine-specific CD8+ T cells in the presence of a Toll-like receptor (TLR) agonist as an adjuvant. (shu.edu)
  • In vivo cell depletion experiments demonstrated that both macrophages and NK cells contribute to the antitumor activity. (bvsalud.org)
  • report that Notch signaling facilitates the generation of DCs that closely resemble their in vivo counterparts and show superior capacity to vaccinate against tumors in vivo. (mssm.edu)
  • However, the mechanisms by which NK cells control tumors in vivo are unclear. (bioxcell.com)
  • Although human ES cells share several molecular features with naive mouse ES cells, they also share a variety of epigenetic properties with primed murine epiblast stem cells (EpiSCs). (nih.gov)
  • Moreover, stable mature protein was preferred to pre-processed antigen or antigen fragments as the substrate for cross-priming. (uniklinik-duesseldorf.de)
  • effect on ceramide production in vivo, presumably due to limited substrate availability, because external addition of very long chain acyl-CoAs resulted in a significant upregulation of very long chain ceramides. (fakpathway.com)
  • Distinct subsets of DCs exist in vivo , broadly divided into conventional (cDC) and plasmacytoid (pDC) subsets. (frontiersin.org)
  • The nanogel-based vaccine significantly inhibited in vivo tumor growth in the prophylactic and therapeutic settings, compared to another vaccine formulation using a conventional delivery system, incomplete Freund's adjuvant. (shu.edu)
  • Efficient antigen cross-presentation through coating conventional alum" by Hongyan Ren, Yongbin Mou et al. (providence.org)
  • Efficient antigen cross-presentation through coating conventional aluminum adjuvant particles with PEI. (providence.org)
  • The novel naive pluripotent cells validated herein retain molecular characteristics and functional properties that are highly similar to mouse naive ES cells, and distinct from conventional primed human pluripotent cells. (nih.gov)
  • Four chromatin marks H3K4me1, H3K4me3, H3K27ac and H3K27me3 were measured in 5 cell lines: C1, WIBR3, LIS2 and WIS2 (naïve and conventional/primed stem cells), and BGO1 (only naïve stem cells). (nih.gov)
  • We hypothesized that blocking access to distinct lymphoid priming sites may alter the specific organ tropism and prevent aGVHD development. (nih.gov)
  • In support of this initial hypothesis, we found that different secondary lymphoid organs (SLOs) imprint distinct homing receptor phenotypes on evolving alloreactive effector T cells in vivo. (nih.gov)
  • Moreover, MVA is reconsidered as prime candidate vaccine for safer protection against orthopoxvirus infections. (uniklinik-duesseldorf.de)
  • Vaccine-induced anti-RH5 serum antibodies exhibited cross-strain functional growth inhibition activity (GIA) in vitro, targeted linear and conformational epitopes within RH5, and inhibited key interactions within the RH5 invasion complex. (jci.org)
  • These findings may be relevant for our understanding of the phenomenon of cross-priming and have obvious implications for vaccine strategies. (ox.ac.uk)
  • Mice primed with M2-DNA channel protein. (cdc.gov)
  • Cross-presentation capacity was assessed in vitro using human DCs and LPs encapsulated in liposomes and in vivo in HLA-A2 transgenic mice (Tgm). (elsevierpure.com)
  • Here, we used reflectance confocal microscopy (RCM) imaging in humans and in mice to visualize tumor architecture in vivo . (bioxcell.com)
  • PCR primers are shown in panel A. The FRT-flanked neomycin selection cassette was excised in vivo by crossing F1 progeny of PRCre chimeras bearing the targeted allele to mice expressing Flpe recombinase systemically (Rodríguez et al. (cdc.gov)
  • To assess the influence of orthonasal and retronasal stimulation on cross-modal interactions between texture and flavor perception of food, a series of experiments have been conducted. (unboundmedicine.com)
  • The retronasal presentation of aroma with concomitant presentation of texture is thought to more closely mimick the in vivo flavor release of semisolid food products as compared to orthonasal stimulation. (unboundmedicine.com)
  • Cross-priming, the result of this process, describes the stimulation of naive cytotoxic CD8+ T cells into activated cytotoxic CD8+ T cells. (wikipedia.org)
  • anti-nucleic acid Ab is used to deliver exogenous Ag to the cross-presentation pathway and inhibit in vivo tumor growth. (technuc.com)
  • Because of this, Bevan implied that these antigen presenting cells must have engulfed and cross presented these foreign MHC cells to host cytotoxic CD8+ cells, thus triggering an adaptive immune response against the grafted tissue. (wikipedia.org)
  • Collectively, the data presented in this thesis reveal the versatile capacity of CD8α+ DCs to interact with various cell types to promote immunity/tolerance and reinforces the notion that targeting Clec9A in vivo is a promising strategy to exploit for immunotherapy. (edu.au)
  • citation needed] However, there is still uncertainty in regard to a mechanistic pathway for cross presentation within an antigen presenting cell. (wikipedia.org)
  • Once the exogenous antigen peptide is loaded onto the MHC class I molecule, the complex is exported to the cell surface for antigen cross presentation. (wikipedia.org)
  • As for the cross-presentation path, the participation of TAP-independent endosomal paths was reported for Hsp90Cpeptide things (9) and for a CTL epitope combined to penetratin, a cell-penetrating peptide extracted from (11). (technuc.com)
  • METHODS: The contribution of immune effector cell subsets behind the antitumor activity of NI-1701 was investigated using flow cytometry, transcriptomic analysis, and in vivo immune-cell depletion experiments. (bvsalud.org)
  • In addition, NI-1701 enhanced dendritic cell-mediated phagocytosis of tumor cells in vitro, resulting in an increased cross-priming of tumor-specific CD8 T cells. (bvsalud.org)
  • On the other hand, it is essential not to paralyze the function of tumor antigen cross-presenting DCs and of the anti-tumor T cell response. (cell-stress.com)
  • We therefore tested multiple cytostatic drugs for their impact on tumor cell killing as well as in in vivo immunization model in which T cell priming requires antigen cross-presentation by activated DCs, using agonist anti-CD40 Abs as the dendritic cell (DC)-activating signal. (cell-stress.com)
  • Secondly, gemcitabine (GEM) and temozolomide, chemotherapeutics drugs generally considered mild and therefore applied in the context of immunostimulatory antibodies in pre-clinical and clinical settings, strongly suppressed DC-dependent T cell priming and expansion. (cell-stress.com)
  • Thus, we have defined a mechanism of NK cell-mediated control of metastases in vivo that may help develop NK cell-dependent cancer therapies. (bioxcell.com)
  • Here we establish defined conditions that facilitate the derivation of genetically unmodified human naive pluripotent stem cells from already established primed human ES cells, from somatic cells through induced pluripotent stem (iPS) cell reprogramming or directly from blastocysts. (nih.gov)
  • However, cross presenting cells are able to utilize the MHC I pathway in order to remain uninfected, while still triggering an adaptive immune response of activated cytotoxic CD8+ T cells against infected peripheral tissue cells. (wikipedia.org)
  • The first evidence of cross-presentation was reported in 1976 by Michael J. Bevan after injection of grafted cells carrying foreign minor histocompatibility (MHC) molecules. (wikipedia.org)
  • Ex vivo Hsp70-activated nk cells in combination with pd-1 inhibition significantly increase overall survival in preclinical models of glioblastoma and lung cancer. (celljournal.org)
  • Comparable to our in vivo observations, fucoidan has been shown to improve CTL activity against NYESO-1 expressing human cancer cells in vitro [19]. (bcrablinhibitor.com)
  • Moreover, transfer of alloreactive effector T cells into conditioned secondary recipients targeted the intestines and liver, irrespective of their initial priming site. (nih.gov)
  • Hallmarks of naive pluripotency include driving Oct4 (also known as Pou5f1) transcription by its distal enhancer, retaining a pre-inactivation X chromosome state, global reduction in DNA methylation and in H3K27me3 repressive chromatin mark deposition on developmental regulatory gene promoters.Upon withdrawal of 2i/LIF, naïve mouse ES cells can drift towards a primed pluripotent state resembling that of the post-implantation epiblast. (nih.gov)
  • This includes competence in the generation of cross-species chimaeric embryos that underwent organogenesis following microinjection of human naive iPS cells into mouse morulas. (nih.gov)
  • For comparison, RRBS was also performed on primed and naïve mouse pluripotent cells. (nih.gov)
  • In vivo administration of HM48-1 antibody can prolong survival of cardiac allografts, an effect which is greatly enhanced by the addition of anti-CD2 mAb 12-15. (bdbiosciences.com)
  • 3D8 scFv effectively shipped a CTL epitope to the proteasome-dependent cross-presentation path in DCs. (technuc.com)
  • The observed cross-modal effect of texture on aroma intensity was not significantly altered by the mode of aroma delivery, i.e., orthonasal or retronasal stimulus administration. (unboundmedicine.com)
  • The ion occupancies derived from the MRD data are substantially higher than previously reported, suggesting that groove-bound ions significantly influence the energetics and structural polymorphism of DNA in vivo. (lu.se)
  • Evidence of in vivo or in vitro genotoxicity often plays an important role in IARC carcinogenicity classifications. (who.int)
  • These results would not have been possible without critical recent innovations in the field, such as the development of safe and efficient materials for in vivo mRNA delivery and advanced protocols for the production of high quality mRNA. (researchgate.net)
  • nevertheless, their delivery of exogenous Ag to the cross-presentation path in APCs offers not really been demonstrated. (technuc.com)
  • Citation: Chickarmane V, Enver T, Peterson C (2009) Computational Modeling of the Hematopoietic Erythroid-Myeloid Switch Reveals Insights into Cooperativity, Priming, and Irreversibility. (lu.se)
  • Latest re-evaluation of the part of Compact disc91 in doctor96-mediated cross-presentation displays the importance of liquid phaseCmediated, than receptor-mediated rather, subscriber base paths and shows the part of heparan sulfate proteoglycans (HSPGs) in surface area 4168-17-6 IC50 joining of doctor96 (10). (technuc.com)
  • SIRPα-Fc-CD40L bound CD47 and CD40 with high affinity and activated CD40 signaling in the absence of Fc receptor cross-linking. (aacrjournals.org)
  • However, the molecular machinery involved in cross-presentation has not been fully defined. (technuc.com)
  • Understanding the underlying molecular circuitry in terms of how it governs both a primed state and, at the other extreme, a committed state is of relevance not only to hematopoiesis but also to developmental systems in general. (lu.se)
  • Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
  • This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes. (bdbiosciences.com)
  • A cross-sectional study method was carried out to explore the differences among the proportions of lung lobe infection and to correlate the percentage of infection (POI) of the whole lung in all patients with clinical laboratory examination values. (biomedcentral.com)
  • In conjunction with late delivery, the effect cross-modal interactions apparently decreased. (unboundmedicine.com)
  • The effects of saliva contamination after acid etching or self-etch priming, and the effect of re-etching or self-etch priming after saliva contamination on shear bond strength were also assessed. (who.int)
  • In addition, the use with previous caesarean section, ruptured of a cervical catheter has been shown ef- membranes, contraindications for vaginal fective for cervical priming and leads to a birth, suspected cephalopelvic dispropor- favourable outcome [ 9 ]. (who.int)
  • An interesting fallout from this architecture is that suppression of the cofactor through external means can lead to a loss of cooperativity, and hence to a primed state for PU.1 and GATA-1. (lu.se)
  • Reduced representation bisulfite sequencing was performed on primed and naïve (expanded in NHSM-WIS medium) human ESCs. (nih.gov)
  • There were no significant differences in shear bond strength between phosphoric acid etching and self-etch priming for no contamination, saliva contamination, and repeat treatment (etching or priming) after saliva contamination. (who.int)
  • Naser Al Deen N, Nassar F, Nasr R, Talhouk R. Cross-roads to drug resistance and metastasis in breast cancer: miRNAs regulatory function and biomarker capability. (celljournal.org)
  • It is the way that attacks the prime cause of cancer most directly and that is experimentally most developed. (tripod.com)
  • As expected, ascites cancer in vivo is not cured. (tripod.com)
  • The superior quality of our InVivo Plus™ products will meet and exceed the strict demands and rigorous standards required for in vivo research. (bioxcell.com)
  • An in vivo method was utilized in order to determine any anti-implantation, early abortifacient and/or foetal resorption effects of these extracts. (who.int)
  • One hundred and thirty extracts as well as one hundred fractions and seven pure compounds were tested by the in vivo method. (who.int)