• Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)
  • The responsibilities of the specialized surface epithelium of the cornea require it to maintain tightly regulated differentiated properties. (molvis.org)
  • This markers include the number of cells in the cornea, the thickness of the corneal epithelium and the presence of nerves. (ca.gov)
  • A 110-115-kD protein is present at levels 27-fold higher in migratory epithelium in the rat cornea than in stationary epithelium. (silverchair.com)
  • The production, preparation, and use of bacterial nanocellulose as corneal bandages could be the key to help delicate stem cells to migrate to the cornea and heal the eye from a range of ocular disorders. (icmab.es)
  • A therapeutic answer can be found in limbal stem cells (LSC), which are located in the corneal limbus and replace lost cells in the cornea. (icmab.es)
  • The size of the pig eyes is quite similar to those of humans and regeneration of the corneal epithelium can be studied because the cells of the cornea are alive during the culture. (icmab.es)
  • Scanning the cornea with a field of view of up to 400 x 400 μm, HRT3 RCM allows you to acquire unique en face images of corneal cells and structures, identify keratocytes subpopulations, and visualize details of the corneal subbasal nerve plexus. (heidelbergengineering.com)
  • Navigate through the cornea at the cellular level and select your preferred scanning depth for a comprehensive in vivo assessment of all corneal layers - from epithelium to endothelium. (heidelbergengineering.com)
  • The comprehensive assessment of the cornea and other external ocular structures with HRT3 RCM can aid you in the diagnosis and monitoring of corneal abnormalities, pre- and post-surgery evaluation, the assessment of dry eye disease, or in the analysis of corneal nerve structure in diabetic patients. (heidelbergengineering.com)
  • The corneal epithelium is maintained by a population of stem cells known as limbal stem cells (LSCs) due to their location in the basal layer of the outer border of the cornea known as the limbus. (ncl.ac.uk)
  • To develop and evaluate a three-dimensional organ culture system of the cornea anterior chamber that could replicate the in vivo processes occurring during corneal wound healing and corneal transplantation. (lancs.ac.uk)
  • This study compared the abnormal corneal epithelium after laser-assisted subepithelial keratectomy (LASEK) with dystrophic cornea using in vivo using confocal microscopy (IVCM) and examined the effects of the abnormal epithelium on postoperative recovery of uncorrected distance visual acuity (UDVA) and sub-basal nerve plexus regeneration. (geneticsmr.com)
  • Although FD-related cornea verticillata due to glycosphingolipids accumulation is considered to be primarily a corneal disease, in vivo confocal microscopy showed structural alterations throughout the entire ocular surface epithelia. (arvojournals.org)
  • This alternative method utilizes reconstructed human cornea-like epithelium which closely mimics the histological, morphological, biochemical and physiological properties of the human corneal epithelium. (nelsonlabsbozeman.com)
  • Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage. (nelsonlabsbozeman.com)
  • The test method evaluates the cytotoxicity induced by a series of test chemical dilutions in a monolayer of rabbit corneal fibroblasts (Statens Seruminstitut Rabbit Cornea - SIRC) after a single five-minute exposure. (iivs.org)
  • Tandem scanning confocal corneal microscopy in the diagnosis of suspected Acanthamoeba keratitis. (cdc.gov)
  • Aims To analyse age-related changes of human conjunctiva by in vivo laser scanning confocal microscopy (LSCM). (bmj.com)
  • On confocal microscopy, distinct deposits are found in the epithelium and Bowman layer. (aao.org)
  • A, Confocal microscopy reveals highly reflective material without shadows in the basal epithelium. (aao.org)
  • Acanthamoeba was detected by culture, smear, and in-vivo confocal microscopy (IVCM) in 25 eyes (56.8%), while in 19 eyes (43.2%) the diagnosis was based solely on the clinical findings. (dovepress.com)
  • The provisional diagnosis of AK is often based on the clinical features and in-vivo confocal microscopy (IVCM). (dovepress.com)
  • Microstructural analysis using confocal laser scanning microscopy evaluated increased corneal thickness, decreased number of cells, increased debris or specific deposits, and unusual microstructures. (scielo.br)
  • In vivo confocal microscopy is a useful tool for evaluating the corneal microstructure when a Kayser-Fleischer ring is clinically present. (scielo.br)
  • Avaliar, ao nível microestrutural, através de microscopia confocal in vivo a lazer, 12 córneas com anel de Kayser-Fleischer visível ao exame da lâmpada de fenda e compará-las com 12 córneas clinicamente normais de indivíduos com idades correspondentes aos pacientes com doença de Wilson e sintomas neurológicos. (scielo.br)
  • Todos os pacientes foram submetidos a um exame clínico abrangente e a uma análise microestrutural subsequente utilizando microscopia confocal in vivo de varredura a laser. (scielo.br)
  • A microscopia confocal in vivo com varredura a laser revelou achados semelhantes em todas as córneas afetadas. (scielo.br)
  • To evaluate the cellular changes in the corneal epithelium and surrounding cells within patients suffering from LSCD, Dr Deng and colleagues examined 27 eyes of 20 patients with in vivo laser scanning confocal microscopy. (ophthalmologytimes.com)
  • To evaluate the microscopic corneal and conjunctival morhpology in patients with Fabry disease (FD) related keratopathy by using in vivo confocal microscopy (IVCM). (arvojournals.org)
  • Corneal and conjunctival morphology were assessed by using a Laser-Scanning confocal Microscope. (arvojournals.org)
  • Confocal microscopy examination revealed two different types of corneal epithelial alterations. (arvojournals.org)
  • Confocal microscopy may assist ophthalmologists in the diagnosis of FD-related ocular surface and corneal manifestations and in the detection of variations while monitoring the effect of enzyme replacement therapy. (arvojournals.org)
  • The basement membrane is also important for epithelial migration, and abnormalities in basement membrane structure, whether due to trauma (eg, recurrent erosion syndrome) or dystrophy (eg, basement membrane dystrophy), can lead to persistence of corneal epithelial defects and stromal ulceration. (medscape.com)
  • Electron microscopy is needed to histologically distinguish RBCD from Thiel-Behnke corneal dystrophy (TBCD), which has curly fibers (see the next section). (aao.org)
  • Figure 7-6 Reis-Bücklers corneal dystrophy. (aao.org)
  • Reis-Bücklers corneal dystrophy appears in the first few years of life and mainly affects the Bowman layer. (aao.org)
  • Novel DCN Mutation in Armenian Family With Congenital Stromal Corneal Dystrophy. (ucla.edu)
  • Recurrent corneal erosion syndrome (RCES) is a relatively common condition and may be classified as either dystrophic (dystrophic-RCES) where it occurs associated with an anterior corneal dystrophy, or non-dystrophic (ndRCES) where it occurs following superficial corneal trauma (post-traumatic RCES) or unrelated to any pre-existing corneal disease or past history of trauma (idiopathic RCES). (bmj.com)
  • Corneal dystrophies commonly associated with RCES include epithelial basement membrane (Cogan's microcystic or map dot fingerprint dystrophy), Reis-Buckler's, lattice, and granular dystrophies. (bmj.com)
  • Examination of Kynurenine Toxicity on Corneal and Conjunctival Epithelium: In vitro and in vivo Studies. (cellsystems.eu)
  • IMPORTANCE Both in vitro and in vivo models are employed for assessing the pandemic potential of novel and emerging influenza A viruses in laboratory settings, but systematic examinations of how well viral titer measurements obtained in vitro align with results from in vivo experimentation are not frequently performed. (cdc.gov)
  • Effect of Scleral Lens Wear on Central Corneal Thickness and Intraocular Pressure in Patients With Ocular Surface Disease. (sc-ctsi.org)
  • The control eyes all presented normal visual acuity, intraocular pressure, and corneal appearance. (scielo.br)
  • In recent years, more and more attention was paid to certain features that appeared as round or oval empty structures within the conjunctival epithelium under LSCM. (bmj.com)
  • The development of high-resolution microscopy techniques indicates nanotopography occurs in vivo, for example in the basement membrane of the corneal epithelia, suggesting its involvement in fundamental cell and tissue processes. (gla.ac.uk)
  • Wound healing and corneal transplantation were observed using light, scanning, and transmission electron microscopy. (lancs.ac.uk)
  • An understanding of the pathophysiology of sterile corneal ulceration requires a review of the processes involved in epithelial and stromal wound healing, as well as an examination of the role of precorneal tear film, corneal nerves, proteolytic enzymes, and cytokines. (medscape.com)
  • According to the EPA, EpiOcular Eye Irritation testm along with BCOP test (Bovine Corneal Opacity and Permeability) and Cytosensor Microphysiometer assaym "can be used to satisfy the in vivo data requirement for eye irritation in 40 CFR Part 158W for Antimicrobial Cleaning Products. (nelsonlabsbozeman.com)
  • The BCOP (Bovine Corneal Opacity and Permeability) assay is an in vitro eye irritation test method developed by Gautheron et al. (iivs.org)
  • 1992), which uses living bovine corneal tissue, obtained as a by-product from abattoirs, to evaluate the potential ocular irritancy of a test article. (iivs.org)
  • 13] Robertson DM,Kalangara JP,Baucom RB,et al.A reconstituted telomerase-immortalized human corneal epithelium in vivo:a pilot study[J].Curr Eye Res,2011,36(8):706-712. (magtech.com.cn)
  • This study also includes preliminary ex vivo evaluation of the BNC on a Porcine Corneal Organ Culture as a simulated transplantation scenario. (icmab.es)
  • Treatment of limbal stem cell deficiency (LSCD) has been achieved with transplantation of ex vivo expanded LSCs taken from a small biopsy of limbus. (ncl.ac.uk)
  • 05). This study demonstrates that transplantation of autologous limbal epithelial stem cells cultured on HAM without the use of non-human animal cells or products is a safe and effective method of reconstructing the corneal surface and restoring useful vision in patients with unilateral total LSCD. (ncl.ac.uk)
  • Development of a Three-Dimensional Organ Culture Model for Corneal Wound Healing and Corneal Transplantation. (lancs.ac.uk)
  • The authors observed that the processes of wound healing and corneal transplantation in the model appeared similar to those occurring in vivo. (lancs.ac.uk)
  • In vitro model closely replicated the in vivo processes of wound healing and corneal transplantation. (lancs.ac.uk)
  • IVCM was effective in detection of Acanthamoeba in cases with early presentation, while culture was more sensitive in late presentation with corneal melting. (dovepress.com)
  • Biomarkers of in vivo limbal stem cell function. (ca.gov)
  • PURPOSE: To evaluate in vivo parameters as biomarkers of limbal stem cell function and to establish an objective system that detects and stage limbal stem cell deficiency (LSCD). (ca.gov)
  • The test method utilizes an in vitro reconstructed human corneal epithelium (RhCE) model (EpiOcular™, MatTek Corp. or HCE, SkinEthic™), in an acute exposure assay to support international regulatory labeling requirements, according to the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS). (iivs.org)
  • The ocular irritation potential of formulations, products, ingredients, and chemicals can be evaluated using in vitro reconstructed human corneal epithelium (RhCE) models, such as the EpiOcular™ (MatTek Corp.) and SkinEthic HCE (EPISKIN) organotypic 3-D tissue constructs. (iivs.org)
  • Recently, the role of the corneal nerve in the pathogenesis of dry eye disease (DED) has been highlighted. (annocl.org)
  • These results suggest that the TEER assay using the human corneal epithelial model is very useful for an ocular irritancy evaluation as an alternative to the Draize eye irritation test. (bio-story.com)
  • An in vitro method for assessment of Ocular Irritation potential of chemicals has been recently accepted by the regulatory authorities as an alternative to in vivo Draize rabbit eye irritation test. (nelsonlabsbozeman.com)
  • The Eye Irritation Test (EIT) is an OECD-approved in vitro non-animal test method for identifying chemicals and mixtures that may be irritating to the corneal epithelium. (iivs.org)
  • The Short Time Exposure (STE) assay, developed by Kao Corporation (Japan), is an in vitro assay used to assess acute eye irritation potential as an alternative to the traditional in vivo Draize test. (iivs.org)
  • The Ocular Irritection Assay is an in chemico eye irritation assay that detects, ranks, and predicts the corneal irritation potential of a test material. (iivs.org)
  • Corneal recovery is a key property of ocular irritation classification schemes used in the regulatory arena. (iivs.org)
  • However, no current validated non-animal ocular irritation test methods or prediction tools are able to predict corneal recovery in a manner consistent with the animal tests historically employed. (iivs.org)
  • To develop such a TEER assay system in vitro utilizing a human corneal epithelial model, HCE-T cells (a human corneal epithelial cell line) were cultured on the collagen vitrigel membrane substratum prepared in a Millicell chamber suitable for TEER measurement. (bio-story.com)
  • Molecular characterization of a new corneal pathogen. (cdc.gov)
  • Wiernas, Terry Kirkham, Characterization of the Bradykinin Receptor in Human Corneal Epithelium. (tdl.org)
  • This electrical activity is often used during embryogenesis, regeneration, and cancer suppression-it is one layer of the complex field of signals that impinge upon all cells in vivo and regulate their interactions during pattern formation and maintenance. (wikipedia.org)
  • There are numerous examples of ocular surface diseases in which the corneal-specific epithelial qualities are not maintained and significant anterior eye physiologic perturbations occur resulting in dramatic loss of vision. (molvis.org)
  • The implant was also tested in vivo on HSV-1-infected rabbit corneas where it was grafted by anterior lamellar keratoplasty. (diva-portal.org)
  • The epithelium was exposed to air, and its anterior surface was automatically irrigated. (lancs.ac.uk)
  • Only very diffuse localization with occasional punctate spots between adjacent superficial cells was present in stationary epithelium. (silverchair.com)
  • This information will be helpful to physicians and clinicians who are in search of additional forms of treatment for intractable ocular surface and corneal abnormalities. (sc-ctsi.org)
  • Numerous cytokines and growth factors that are up-regulated in corneal cells further contribute to tissue inflammation. (medscape.com)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • As the relative contribution of in vitro experimentation to these rubrics has not been closely examined, we sought to evaluate to what extent viral titer measurements over the course of in vitro infections are predictive or correlates of nasal wash and tissue measurements for IAV infections in vivo. (cdc.gov)
  • Half the PKP specimens were debrided, and half were left with the epithelium in situ. (unicatt.it)
  • During this training, she invented several natural biopolymers based in situ forming hydrogels to promote corneal wound healing. (stanford.edu)
  • Conclusions: The data suggest PNN's role in the alternative splicing of a specific subset of lncRNAs might have a significant impact on the corneal epithelium. (molvis.org)
  • CONCLUSIONS: The HPLC quantitative study showed that stromal concentrations of riboflavin increased with exposure time only if the epithelium was removed. (unicatt.it)
  • Hurt M, Niederkorn J, Alizadeh H. Effects of mannose on Acanthamoeba castellanii proliferation and cytolytic ability to corneal epithelial cells. (cdc.gov)
  • Aligned and stacked cells (such as in epithelia) generate transepithelial potentials (such as batteries in series) and electric fields, which likewise propagate across tissues. (wikipedia.org)
  • Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
  • To block viral reactivation, we incorporated silica dioxide nanoparticles releasing KR12, the small bioactive core fragment of LL37, an innate cationic host defense peptide produced by corneal cells. (diva-portal.org)
  • The corneal epithelium is composed of stratified squamous epithelial cells on the outer surface of the eye, which acts as a protective barrier and is critical for clear and stable vision. (wjgnet.com)
  • Within minutes after a small corneal epithelial injury, cells at the edge of the abrasion begin to migrate centripetally to cover the defect rapidly at a rate of 60-80 µm/h. (medscape.com)
  • Human corneal epithelium model possessing 5-6 cell layers sufficient for TEER assay was successfully reconstructed on the substratum in the Millicell chamber by culturing the cells in monolayer for 2 days and subsequently in air-liquid interface for 7 days. (bio-story.com)
  • The present invention provides methods of achieving directed evolution of viruses by in vivo screening or "panning" to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and/or neutralization profile (e.g., ability to evade neutralizing antibodies). (justia.com)
  • The invention relates to methods for directed evolution and in vivo panning of adeno-associated virus vectors as well as optimized AAV capsids and virus vectors comprising the same. (justia.com)
  • Methods: Eight patients who failed primary phototherapeutic keratectomy for recurrent corneal erosion syndrome were treated with repeat phototherapeutic keratectomy. (bmj.com)
  • The ring consists of granular, bright particles that increase in density toward Descemet's membrane, and is associated with a decreased number of keratocytes and peculiar dark, round areas in all stromal layers, probably a sign of corneal damage. (scielo.br)
  • Eight eyes of eight consecutive patients with unilateral total LSCD treated with ex vivo expanded autologous LSC transplant on human amniotic membrane (HAM) with a mean follow-up of 19 (RANGE) months were included in the study. (ncl.ac.uk)
  • It is still unclear weather the different type of corneal epithelial lesions observed for hemizygous and heterozygous patients is to be related to different physiopathological mechanisms. (arvojournals.org)
  • Conclusion: Re-treatment with phototherapeutic keratectomy appears to be successful for patients with macroerosions complicating recurrent corneal erosion syndrome who have failed conservative management with ocular lubricants and a primary phototherapeutic keratectomy. (bmj.com)
  • The assay assesses changes to the reagent solution (containing proteins, glycoproteins, lipids), which mimics the denaturation and disruption that occurs in corneal proteins in vivo . (iivs.org)
  • Pseudomonas aeruginosa-Derived Extracellular Vesicles Modulate Corneal Inflammation: Role in Microbial Keratitis? (elsevierpure.com)
  • 5 The tear film, lacrimal glands, corneal and conjunctival epithelia and Meibomian glands work together as an integrated lacrimal functional unit (LFU). (touchophthalmology.com)
  • PURPOSE: To evaluate intrastromal concentrations of riboflavin with and without epithelium to ensure the efficacy and safety of corneal crosslinking (CXL) by the standard and transepithelial procedures. (unicatt.it)
  • In Western blots comparing proteins from migratory and control epithelium, antibody against vinculin cross-reacted with the 110-115-kD protein. (silverchair.com)
  • In flat mounts of these whole wounded corneas, vinculin was localized as punctate spots in the leading edge of migrating epithelium. (silverchair.com)
  • She also established two corneal disease models in rodents and large animals with surgical tool-inner stopper trephines, which she invented to create consistent corneal defects in animal corneas. (stanford.edu)
  • The brief TEER (trans-epithelial electrical resistance) assay after exposing chemicals to corneal epithelium in vivo is known as a suitable method for evaluating corneal irritancy and permeability quantitatively and continuously. (bio-story.com)
  • Grasp the nictitating membrane at its apex with 0.3 forceps and pull it over the corneal surface. (jove.com)
  • When ready, replace the wire speculum, grasp and extend the nictitating membrane over the corneal surface with the forceps, and cut the membrane at it's base with tenotomy scissors. (jove.com)
  • A collagen vitrigel membrane we previously developed is a thin (about 20 μm thick) and transparent membrane composed of high density collagen fibrils equivalent to connective tissues in vivo , e.g. corneal Bowman's membrane. (bio-story.com)
  • Surgical intervention in the form of tectonic grafts or amniotic membrane transplant was required in five cases (11.3%) due to progressive corneal thinning and perforation. (dovepress.com)
  • Com a hipótese de que a associação da INS à lipossomas aumente a biodisponibilidade ocular da INS e auxilie na proteção das células do epitélio corneano, lesionadas nos casos de DOS, o objetivo deste trabalho foi avaliar, in vivo, o efeito de lipossomas contendo INS (lipossoma-INS) nos principais sinais da DOS: secreção lacrimal diminuída, células epiteliais agredidas, epitélio fino e inflamação. (usp.br)
  • Hubschman JP, Govetto A, Farajzadeh M, Sato T, Askari S, Glasgow B. Feasibility of a polyethylene glycol-derived polymer as retinal patch to seal retinal breaks during vitrectomy for rhegmatogenous retinal detachment: a prospective, in vivo pilot study in a porcine model. . (ucla.edu)
  • When the ring is not visible in subjects with Wilson's disease, changes to the corneal microstructure are insignificant. (scielo.br)
  • Cellular tropism is similar to other morbilliviruses, and PoMV viral RNA was detected in neurons, respiratory epithelium, and lymphocytes. (cdc.gov)
  • Mathematical modeling was used to estimate more generalizable key replication kinetic parameters from raw in vitro viral titers, revealing commonalities between viral infection progression in vivo and in vitro. (cdc.gov)