• We propose that BMI1 plays an important role in enforcing Treg identity in vitro and in vivo. (jci.org)
  • T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
  • Emperipolesis is amplified in models of murine inflammation associated with platelet overproduction, contributing to platelet production in vitro and in vivo. (elifesciences.org)
  • Mainly analyzed by in vitro assays in tumor transplantation models, little is known about their function in autochthonous tumor models in vivo. (mdc-berlin.de)
  • Notably, genetic Ae2 silencing in OVA-specificT cells improvedCD4(+)/CD8(+) T cell function in vitro as well as their antitumor activity in vivo. (unav.edu)
  • Post electroporation, cells were resuspended in NK cell media and transferred to culture flasks for in vitro analysis. (maxcyte.com)
  • Proliferative/expansion potential, phenotypical characteristics and functionality of the propagated CD19CAR T cells were analyzed in vitro and in vivo after 17-21 day ex vivo expansion. (biomedcentral.com)
  • QL-007 significantly inhibited HBV replication in a dose-dependent manner both in vitro and in vivo, resulting in significant decreases in HBV DNA, 3.5 kb HBV RNA and HBeAg. (bvsalud.org)
  • In vitro cytotoxicity assays and in vivo adoptive transfer experiments were performed to determine the cytotoxicity of high and low avidity populations. (bmj.com)
  • For that aim, we functionally characterize these targets in vitro and in vivo. (leloir.org.ar)
  • Blockade of TGF-beta in vitro and in vivo interfered with immunosuppression. (nih.gov)
  • CD4 + cells from the skin-draining lymph nodes (SDLN) of either 1,25(OH) 2 D 3 -treated or UVB-irradiated mice had reduced capacity to proliferate to Ags presented in vitro, and could suppress Ag-specific immune responses upon adoptive transfer into naive mice. (aai.org)
  • Furthermore, purified CD4 + CD25 + cells from the SDLN of 1,25(OH) 2 D 3 -treated or UVB-irradiated mice compared with equal numbers of CD4 + CD25 + cells from control mice had increased capacity to suppress immune responses in both in vitro and in vivo assay systems. (aai.org)
  • Functional studies revealed that HOXA5 upregulation impaired the stem-like characteristics and malignant behaviors of PCa cells in vitro and in vivo. (bvsalud.org)
  • We established an in vivo BLM (3.5 mg/kg)-induced PF murine model and in vitro BLM (35 µM)-damaged MLE-12 cell model. (bvsalud.org)
  • Interest therefore exists in enhancing immune reconstitution through regenerative or cell therapies for boosting thymus activity in vivo, or providing customized in vitro generated T cell repertoires for adoptive transfer. (ed.ac.uk)
  • We also tested our hypothesis that intratumoral (i.t.) delivery of dendritic cells that had been transduced with IFN-alpha cDNA (DC-IFN-alpha) would enhance the tumor-homing and antitumor effectiveness of adoptively transferred Tc1 via induction of an IFN-gamma-inducible protein 10 (IP-10). (nih.gov)
  • In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
  • 3 Autologous lymphocytes genetically modified with the TCR for MART-1/Melan-A have been adoptively transferred to patients with advanced melanoma. (haematologica.org)
  • These analyses revealed the coordinated functional dynamics of the adoptively transferred, as well as endogenous, T cells, and the importance of highly functional T cells in dominating the antitumor immune response. (ca.gov)
  • Magnetic targeting of adoptively transferred tumour-specific nanoparticle-loaded CD8+ T cells does not improve their tumour infiltration in a mouse model of cancer but promotes the retention of these cells in tumour-draining lymph nodes. (csic.es)
  • While increased CD16 expression was transient in nature, this may not negatively impact the therapeutic potential of engineered NK cells due to the short persistence of adoptively transferred NK cells. (maxcyte.com)
  • Once adoptively transferred into CD19+ tumor-bearing mice, Akti treated CD19CAR T cells exhibited more antitumor activity than did untreated CD19CAR T cells. (biomedcentral.com)
  • Enhancing the in Vivo Expansion of Adoptively Transferred EBV-Specific CTL with Lymphodepleting CD45 Monoclonal Antibodies in NPC Patients. (texaschildrens.org)
  • The CD4(+) T cells expressed both cell surface and soluble TGF-beta and inhibited the development of an allergic phenotype when adoptively transferred to naive recipient mice. (nih.gov)
  • Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
  • Les IgIV intéragissent avec de nombreux composants du système immunitaire comme les récepteurs Fc, le complément, les cytokines, les lymphocytes T et B, les cellules dendritiques, les granulocytes et les cellules NK, ce qui explique en partie leurs effets anti-inflammatoires. (academie-medecine.fr)
  • Adoptive cell transfer therapy using CD8+ T lymphocytes showed promising results eradicating metastatic malignancies. (unav.edu)
  • Cloned populations of antigen-specific, murine T-lymphocytes, maintained in long-term continuous culture, have been reported to exhibit functional activity upon adoptive transfer. (psu.edu)
  • Adoptive T cell therapy (ACT) with tumor infiltrating lymphocytes (TIL), T cell receptor (TCR) and Chimeric Antigen Receptor (CAR) engineered T cells, or hematopoietic stem cell transplantation, is a promising new approach to cancer treatment. (nih.gov)
  • Moreover, lymphocytes collected from recipients transferred by lymphocytes from transgenic mice revealed less proliferative potential than lymphocytes obtained from control recipients. (diabetesjournals.org)
  • The transfer of lymphocytes or ethylene di-isocyanate (HDI), which are used in a serum from sensitized mice can cause clinical disease in naive variety of industries including polyurethane foam mice. (cdc.gov)
  • Using C57BL/6 mice bearing intracranial (i.c.) ovalbumin-transfected melanoma (M05), we evaluated the efficacy and tumor homing of i.v. transferred type 1 or 2 CTLs (Tc1 or Tc2, respectively) prepared from ovalbumin-specific T-cell receptor-transgenic OT-1 mice. (nih.gov)
  • 15 Several second generation anti-CD20 antibodies have recently been developed, and clinical trials are currently ongoing to investigate their in vivo efficacy. (haematologica.org)
  • We have established a new humanised mouse model of lupus nephritis that enables us to test the efficacy of our targeted Tregs in vivo. (lupusresearch.org)
  • Early studies on the transfer of unmodified autologous or allogeneic NK cells have established their clinical safety and demonstrated modest anti-tumor efficacy. (maxcyte.com)
  • Anti-tumor IgG administered intravenously to mice eliminated a fraction of CD47 knockout tumors in vivo but had no effect on wild-type tumors. (aiche.org)
  • In vivo, DIDS administration delayed B16OVA tumor growth in immunocompetent mice as monotherapy or when combined with adoptive T cell transfer of OVA-specificT cells. (unav.edu)
  • Using HLA-A2.1 transgenic mice, we have demonstrated the feasibility of T-cell receptor (TCR) gene transfer into T cells to circumvent self-tolerance to the widely expressed human p53 and MDM2 TAAs. (unimedizin-mainz.de)
  • In this model, the adoptive transfer of PBMCs from SLE patients with lupus nephritis into immunocompromised NSG-MHC null mice lead to the development of nephritis. (lupusresearch.org)
  • Similarly, CD8 T cells transferred into tumor-bearing mice upregulate FOXP3 in vivo. (unav.edu)
  • Anti-tumor activity was evaluated after adoptive transfer of the CD19CAR T cells into CD19+ tumor-bearing immunodeficient mice. (biomedcentral.com)
  • For immunological studies, we use different animal and cellular models (i.e. knock-out mice, OT-1 and OT-2 transgenic mice, adoptive transfer of immunity, models of protective and therapeutic vaccine against tumors, in vivo analysis of antigen persistence in cell and tissues). (leloir.org.ar)
  • Uninfected or Leishmania -infected thioglycollate-elicited peritoneal exudate cells were transferred from C57BL/6 to BALB/c mice or from Ly5.1 + to Ly5.1 - mice. (biomedcentral.com)
  • Using newly developed transgenic mice expressing a bioluminescent ATP probe on the cell surface, we found that extracellular ATP levels increase during sepsis, indicating that ATP may contribute to bacterial killing in vivo. (jci.org)
  • Results with adoptive transfer of macrophages, myeloid-specific P2X4R-deficient mice, and P2rx4 tdTomato reporter mice indicate that macrophages are essential for the antibacterial, antiinflammatory, and organ protective effects of P2X4Rs in sepsis. (jci.org)
  • Following the sensitization of recipient mice with OVA, the proportion of CD4 + Foxp3 + cells of donor origin significantly increased in recipients of CD4 + CD25 + cells from the SDLN of 1,25(OH) 2 D 3 -treated mice, indicating that these regulatory T cells can expand in vivo with antigenic stimulation. (aai.org)
  • Adoptive cell transfer (ACT) of genetically engineered T cells expressing cancer-specific T-cell receptors (TCR) is a promising cancer treatment. (ca.gov)
  • Lessons learned from the study of NK cell memory could help with the design of better expansion protocols for adoptive NK cell therapy, for the manufacturing of chimeric antigen receptor (CAR)-engineered NK cells and for improving NK cell-based therapies that rely on antibody-dependent cellular cytotoxicity (ADCC). (nature.com)
  • Insufficient persistence and effector function of chimeric antigen receptor (CAR)-redirected T cells have been challenging issues for adoptive T cell therapy. (biomedcentral.com)
  • T-cell transfer therapy: a treatment that takes T-cells from the tumor and selects or changes them in the lab to better attack cancer cells, then reintroduces them into the patient. (wikipedia.org)
  • In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
  • In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
  • In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
  • In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
  • Finally, BMI1 mutant FOXP3+ cells did not suppress colitis in the adoptive transfer model of human inflammatory bowel disease. (jci.org)
  • The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)
  • Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
  • CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
  • Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
  • Transfer of HSV-tk to DLI preserves the beneficial anti-tumor effect and allows in vivo elimination of donor T cells if severe GvHD occurs. (haematologica.org)
  • 4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
  • In this study, we investigated the use of CD20 as safety switch after retroviral transfer to T cells with different cytomegalovirus (CMV) specificities, and conclude that CD20 may be broadly applicable as safeguard in adoptive T-cell therapy. (haematologica.org)
  • This manuscript describes the application of newly developed nanodiagnostic assays to analyze the function of immune cells used in adoptive cell transfer for patients with melanoma. (ca.gov)
  • Here, we investigate the in vivo functional activity and dynamics of the transferred cells by analyzing samples from 3 representative patients with melanoma enrolled in a clinical trial of ACT with TCR transgenic T cells targeted against the melanosomal antigen MART-1. (ca.gov)
  • One approach relies on the isolation and cloning of T cell receptors (TCRs) that confer non-reactive T cells with specific and potent immune function following gene transfer. (upenn.edu)
  • The adoptive transfer of OVA-specificT cells overexpressing Hvcn1 exerted a better antitumor activity in B16OVA tumor-bearingmice. (unav.edu)
  • Adoptive cell transfer (ACT) is the transfer of cells into a patient. (richardvigilantebooks.com)
  • vivo activation and expansion of T cells or NK cells and passive transfer of these cells into the cancer patient. (richardvigilantebooks.com)
  • Cells for adoptive therapy can be obtained either from the patient or from a separate individual. (richardvigilantebooks.com)
  • A safety concern of TCR gene transfer is the risk of pairing between introduced and the naturally expressed endogenous TCR chains, resulting in the generation of self-reactive T cells. (unimedizin-mainz.de)
  • Cell-intrinsic loss of FOXP3 by CD8+ T cells resulted in improved functionality after TCR stimulation and better antitumor responses in vivo. (unav.edu)
  • Only 10% of the human population is homozygous for this polymorphism, suggesting that genetic manipulation of NK cells to express CD16-158V prior to adoptive transfer may improve clinical success not only of NK cell therapies but also anti-tumor IgG1 antibodies. (maxcyte.com)
  • Non-viral engineering of NK cells using MaxCyte ® mRNA electroporation provides significant benefits including high efficiency and low toxicity as well as clinical scalability enabling rapid development of novel adoptive cell therapy approaches. (maxcyte.com)
  • NK cells were isolated from healthy peripheral blood mononuclear cells (CD16-158F/F donors) and expanded ex vivo for 11-15 days with irradiated EBV-SMI-LCL cells. (maxcyte.com)
  • Ex vivo expanded NK cells electroporated with mRNA encoding the high-affinity Fc receptor, CD16-158V, demonstrated a significant increase in CD16 surface expression (Figure 1A). (maxcyte.com)
  • Ex vivo expanded NK cells were electroporated with mRNA (4 µg/10 6 NK cells) encoding the high-affinity, CD16-158V Fc receptor. (maxcyte.com)
  • Non-electroporated, ex vivo expanded NK cells were used as a control to assess endogenous CD16 expression. (maxcyte.com)
  • In contrast, ACT expands a patient's own T cells ex vivo to direct anti-tumor immunity when transfused back into the patient. (elifesciences.org)
  • We now investigate whether inhibition of Akt signaling during ex vivo expansion of CAR T cells can promote the generation of CAR T cells with enhanced antitumor activity following adoptive therapy in a murine leukemia xenograft model. (biomedcentral.com)
  • Manufacturing CAR T cells is a process that involves activation and ex vivo culture with IL-2 to facilitate CAR gene transfer and to achieve a therapeutic number of T cells. (biomedcentral.com)
  • The concept of enhancing cellular immunity through the transfer of ex-vivo expanded T cells was pioneered by Greenberg et al. (biomedcentral.com)
  • BACKGROUND: Adoptive transfer of virus-specific T cells may accelerate reconstitution of antigen-specific immunity and limit the morbidity and mortality of viral infections following allogeneic hematopoietic stem cell transplantation. (ox.ac.uk)
  • Expansions of both CD4(+) and CD8(+) cytomegalovirus-specific T cells were observed in vivo within days of adoptive transfer. (ox.ac.uk)
  • The transferred cells were injected into the peritoneal cavity and tracked to the draining lymph node. (biomedcentral.com)
  • Coincubation with Leishmania changes the migratory pattern of dendritic cells in vivo . (biomedcentral.com)
  • Adoptive Transfer of EBV-Specific T Cells Results in Sustained Clinical Responses in Patients with Locoregional Nasopharyngeal Carcinoma. (texaschildrens.org)
  • aAPCs on the quality of the extended Capital t cells for adoptive transfer possess however to become identified. (bioskinrevive.com)
  • A recent study by Passerini et al (2013) highlights the possibility of using adoptive cell therapy with genetically engineered regulatory T cells as a novel therapy for IPEX syndrome. (medscape.com)
  • Inhibition of Akt signaling during ex vivo priming and expansion gives rise to CD19CAR T cell populations that display comparatively higher antitumor activity. (biomedcentral.com)
  • Here, we evaluated the safety issues raised by the risk of TAA-TCR gene transfer-associated on/off-target toxicities and the therapeutic potential in relevant transgenic mouse models of adoptive transfer. (unimedizin-mainz.de)
  • This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. (elifesciences.org)
  • For bacterial in vivo characterization, we evaluate the biological relevance of our targets in the host-microbe interaction: we use genome site directed mutagenesis, homologous and heterologous complementation with wild-type and mutant versions of the proteins, classical microbiological determination assays (i.e. biofilm formation, EPS content, flagellar apparatus production, motility, survival to stresses, growth, etc). (leloir.org.ar)
  • These data suggest that Tc1-based adoptive transfer therapy may represent an effective modality for CNS tumors, particularly when combined with strategies that promote a type 1 polarized tumor microenvironment. (nih.gov)
  • Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
  • First, since the B-cell products, antibodies, can be transferred humorally, and should be tolerated by the general population, many existing treatments simply involve injecting antibodies into the patient's bloodstream. (stackexchange.com)
  • Few data are currently available on the in vivo distribution of these homogenous, functional cell populations. (psu.edu)
  • Two of the primary immunotherapies are immune checkpoint blockade (ICB) and adoptive cell transfer (ACT). (elifesciences.org)
  • T cell receptor (TCR) transfer experiments indicate that a major part of avidity is hardwired within the structure of the TCR. (mdc-berlin.de)
  • None of the iMC from the different tumor models suppressed CTL responses in adoptive cell transfer experiments, unless GM-CSF was provided in vivo. (mdc-berlin.de)
  • Therefore, ex vivo NK cell expansion protocols are currently being developed. (frontiersin.org)
  • The possibility to generate Th17 ex vivo has facilitated the elucidation of the signals and transcription factors required for their differentiation and functions and has allowed for the evaluation of their functions following adoptive transfer in vivo. (arizona.edu)
  • Adoptive immunotherapy is a promising therapeutic approach for the treatment of chronic infections and cancer. (mdc-berlin.de)
  • These data indicate that koff rate measurements can improve the predictability of adoptive immunotherapy and provide diagnostic information on the in vivo quality of T cells. (mdc-berlin.de)
  • This article summarizes recent evidence in the literature on the peculiar features that render memory NK cells an attractive tool for antitumor immunotherapy, including their long-term survival and in vivo persistence, the resistance to tumor-dependent immunosuppressive microenvironment, the amplified functional responses to IgG-opsonized tumor cells, and in vitro expansion capability. (hindawi.com)
  • Along with highlighting these issues, we speculate that memory NK cell-based adoptive immunotherapy settings would greatly take advantage from the combination with tumor-targeting therapeutic antibodies (mAbs), as a strategy to fully unleash their clinical efficacy. (hindawi.com)
  • These findings establish Cish as a novel targetable inhibitory molecule demonstrating regulatory properties in tumor-specific CD8+ T cells, which can be manipulated to improve adoptive cancer immunotherapy. (nih.gov)
  • By developing these three Aims for CAR T cells, we hope to not only further advance adoptive T cell therapy but also develop a tool box for metabolic reprogramming that can be exploited to enhance cancer immunotherapy as well as a vast array of immunologically engineered systems. (jhmi.edu)
  • Dr. Childs' lab also has a major focus on NK Cell immunotherapy, conducting lab based and clinical research aimed at exploring a variety of different strategies to optimize adoptive NK cell therapy for cancer. (nih.gov)
  • A commentary that appeared in the same issue of the journal in which the NIH study was published, reported: "The clinical application of T cell immunotherapy depends on ex vivo modification and expansion of T cells for adoptive transfer. (nih.gov)
  • Adoptive immunotherapy (AIT) using this supernatural T cell have gained momentum after decades of intense debates because of the promising results obtained from preclinical models and clinical trials. (biomedcentral.com)
  • In this review, we focus on the critical issues related to the clinical outcomes of CAR-based adoptive immunotherapy and discuss the rationales to refine this new cancer therapeutic modality. (biomedcentral.com)
  • Adoptive immunotherapy (AIT) using chimeric antigen receptor-engineered T cells is a promising approach in cancer treatment. (biomedcentral.com)
  • Besides these beneficial effects, the technical feasibility of CAR makes it even more attractive in the development of cancer adoptive immunotherapy. (biomedcentral.com)
  • T-cell receptor (TCR) gene transfer for cancer immunotherapy is limited by the availability of large numbers of tumor-specific T cells. (eur.nl)
  • In vivo expansion and persistence of adoptively transferred CAR T-cells are required for beneficial antitumor clinical responses in treated patients. (massgeneral.org)
  • Finally, IL10- deficient iNKT cells failed to control the pathogenic activity of adoptively transferred CD4+ T cells in an experimental colitis model. (ecco-ibd.eu)
  • By adoptively transferring sub-optimal doses of CAR T cells (where tumor growth begins to fail), they have successfully compared the potency and persistence of different CAR T cell constructs. (jhmi.edu)
  • In this regard, his group has cloned T-cell receptors recognizing HERV-E antigens and in a first in human clinical trial being conducted at the NIH Clinical Center, is investigating the anti-tumor potential of adoptively transferring autologous T-cells genetically modified to express HERV-E T-cell receptors. (nih.gov)
  • In-vivo tracking of adoptively transferred natural killer-cells in rhesus macaques using 89Zirconium-oxine cell labeling and PET imaging. (nih.gov)
  • A variety of in vivo and more recently in vitro assays have been described to assess cell mediated immunity (CMI). (nih.gov)
  • Knowledgeable of secondary ex vivo cell-based assays across various technology platforms (e.g. (nih.gov)
  • Evidence from a cuprizone-induced model of demyelination, in vitro and in vivo T-cell assays and EAE adoptive transfer experiments indicated that the observed efficacy of this drug results directly from an enhancement of remyelination rather than immune suppression. (ca.gov)
  • NK cell ex vivo and in vivo tumor killing was measured by chromium-release and adoptive transfer assays, respectively. (biomedcentral.com)
  • The disruption of Cish using short hairpin microRNA's was characterized by overall improved sensitivity to cognate antigens, functional avidity, and cytokine productivity in both in vitro co-culture and in vivo adoptive transfer experiments. (nih.gov)
  • We show that PKCη is required for the Treg-mediated suppression of tumor immunity in vivo. (jci.org)
  • The frequency of anti-Dsg3 IgG production depended on the number of transferred naive splenocytes. (elsevierpure.com)
  • Adoptive transfer of Tregs will then be done to restore gut homeostasis. (nih.gov)
  • Hands-on experience and knowledge of humanized models, cell line-derived xenograft models, genetically engineered mouse models, adoptive immune cell transfer models, and/or in vivo genetic delivery platforms. (nih.gov)
  • These data provide the first in vivo genetic evidence that ICC integrate intestinal pacemaker activity with enteric neurotransmission to control and coordinate digestive activity in adult animals. (nature.com)
  • We will measure gut permeability in vivo, tight junction proteins and endotoxin levels in the circulation. (nih.gov)
  • Uses detailed knowledge of state-of-the-art tools and approaches to enhance in vivo evaluation of novel therapeutics mechanism of action and/or identify new targets. (nih.gov)
  • Adoptive cell therapy (ACT) is an attractive new therapeutic approach for treating various cancers. (nih.gov)
  • With the help of this novel model, we analysed ICC function in vivo by genetic loss-of-function studies. (nature.com)
  • Interleukin-12 (IL-12) gene transfer enhances the therapeutic potency of adoptive T cell therapies. (unav.edu)
  • We sought to identify ex vivo culture conditions that would be suitable for the clinical expansion of polyfunctional HER2 specific Th1/Th17 for therapeutic infusion. (biomedcentral.com)
  • Molecular imaging enables non-invasive visualization of the dynamics of molecular processes within living organisms in vivo . (biomedcentral.com)
  • European Society for Molecular Imaging defines molecular imaging as the characterization of the dynamics of the molecular processes in the living organisms in vivo . (biomedcentral.com)
  • How to Choose Between in Vivo and ex Vivo Strategies? (chipsbooks.com)
  • Lentiviral vector-based gene delivery systems have shown increased transfer efficiency and transgene expression compared with the widely used gamma-retroviral vectors. (eur.nl)
  • These links provide resources to those interested in the technology transfer activities at NIH. (nih.gov)
  • These links provide information about the Office of Technology Transfer. (nih.gov)
  • Extensive experience with T cell exhaustion, CAR-T, and in vivo targeted gene delivery. (nih.gov)
  • With the development of sequencing technology, transfer RNA (tRNA)-derived small RNAs (tsRNAs) have received extensive attention as a new type of small noncoding RNAs. (bvsalud.org)
  • Investigations may entail both human and animal studies, and both in vitro and in vivo analyses. (nih.gov)
  • The 53-6.7 antibody exhibits depleting activity when used in vivo . (bioxcell.com)
  • These topics range from general technology transfer information to processes specific to NIH. (nih.gov)
  • The NIH Office of Technology Transfer (OTT) plays a strategic role by supporting the patenting and licensing efforts of our NIH ICs. (nih.gov)
  • To study its role in oncogenic progression, we developed a selective small-molecule RIP1 inhibitor with high in vivo exposure. (bioxcell.com)