• Further described are anti-VAP-1 monoclonal antibodies and methods for the diagnosis and treatment of inflammatory and autoimmune diseases by the administration of VAP-1 binding compounds, such as anti-VAP-1 antibodies. (justia.com)
  • Monoclonal antibodies against KS cells are also provided, as are methods for evaluating antimalignancy therapies. (justia.com)
  • 23. Discrimination of different forms of the murine urokinase plasminogen activator receptor on the cell surface using monoclonal antibodies. (nih.gov)
  • This is incredibly useful for measuring the concentration of important biomolecules, such as developing a quantitation assay for monoclonal antibodies. (heelzup.com)
  • Green Mountain Antibodies utilizes an Octet analysis system for developing monoclonal antibodies which help us deliver protein quantitation with a higher level of accuracy and produce results quickly and efficiently. (heelzup.com)
  • Protein extraction and western blot Cells have been grown to confluency in 35 mm culture dishes. (plcsignaling.com)
  • Using two affinity chromatography columns to purify fCD23, and we detected 44-kDa monomer fCD23 and cross-linked oligomer fCD23 by western blot analysis. (ncl.edu.tw)
  • Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. (takarabio.com)
  • Anti-Integrin alpha-3 (ITGA3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. (bicellscientific.com)
  • Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state. (bicellscientific.com)
  • An acidic amino acid features in the integrin-interaction site of many ECM proteins, for example as part of the amino acid sequence Arginine-Glycine-Aspartic acid ("RGD" in the one-letter amino acid code). (wikipedia.org)
  • Antibody blocking experiments showed that adhesion of both cell types to fibronectin was mediated by the alpha 5 beta 1 integrin and to laminin by alpha 3 beta 1 in synergy with alpha 2 beta 1. (rupress.org)
  • they also expressed alpha 1 beta 1, an integrin that was not consistently detected on normal keratinocytes. (rupress.org)
  • The PAI-vitronectin complex binds to the Arg-Gly-Asp motif of αv integrins and inhibits the integrin-mediated cell migration [ 28 - 33 ]. (biomedcentral.com)
  • Hybridoma cell line, ATCC HB 11029, which produces murine monoclonal antibody, 10C4.1.3 which specifically binds to alphavbeta3 integrin on human osteoclasts, and is capable of blocking binding of alphavbeta3 integrin to fibrinogen or to vitronectin. (justia.com)
  • The antibody is useful for detecting alphavbeta3 integrin on cells in histological and diagnostic assays, and to treat disease conditions that are characterized by excessive bone resorption. (justia.com)
  • IMD-4482 inhibited in vitro cell adhesion to vitronectin in PAI-1-positive ovarian cancer cells, followed by the inhibition of extracellular signal-regulated kinase and focal adhesion kinase phosphorylation through dissociation of the PAI-urokinase receptor complex from integrin αVβ3. (oncotarget.com)
  • Detects human Integrin alpha 2b/CD41 in direct ELISAs. (rndsystems.com)
  • Integrin alpha 2b associates with Integrin beta 3 to form complexes that interact with Fibrinogen, von Willebrand factor, Fibronectin, and Vitronectin. (rndsystems.com)
  • These experiments identified proteins called MMP14, Notch3, and β1-integrin as critical to the invasive spread of melanoma cells. (elifesciences.org)
  • Integrin alpha-3 proteins are expressed by the distal tubules in the mouse kidney, which lack the expression of LTL. (bicellscientific.com)
  • FFPE section of mouse kidney shows integrin alpha-3 protein localization in the podocytes of the glomerulus. (bicellscientific.com)
  • An integrin molecule is composed of two protein subunits, alpha and beta. (bicellscientific.com)
  • Integrin alpha-3 protein is encoded by the ITGA3 gene in human. (bicellscientific.com)
  • Second, there is a strong functional hypothesis: ITGAV encodes the protein α v (CD51 antigen) of the integrin family, which combines with β 3 to form the vitronectin receptor [ 3 ] and has a central role in angiogenesis [ 4 ]. (biomedcentral.com)
  • Utilizing this method has increased Green Mountain Antibodies production capability and improved antibody production by monitoring developing cell lines. (heelzup.com)
  • Best Practices for Aggregate Quantitation of Antibody Therapeutics by Sedimentation Velocity Analytical Ultracentrifugation. (nih.gov)
  • Mini-abstract: This Unit introduces the basic principles and practice of sedimentation velocity analytical ultracentrifugation for the study of reversible protein interactions, such as the characterization of self-association, heterogeneous association, multi-protein complexes, binding stoichiometry, and the determination of association constants. (nih.gov)
  • Secretion of PAI-1 from glial cultures was detected by ELISA and western blotting analysis. (biomedcentral.com)
  • In IgE and CD23 binding ability test, we used binding assay and ELISA to detect fCD23 binding ability. (ncl.edu.tw)
  • Why Converting an ELISA into An Octet Protein Quantitation Assay Is Better? (heelzup.com)
  • An enzyme-linked immunosorbent assay (ELISA) requires a capture antibody and detection antibody to create a "sandwich" with the biomolecule of interest in the middle. (heelzup.com)
  • Integrins work alongside other proteins such as cadherins, the immunoglobulin superfamily cell adhesion molecules, selectins and syndecans, to mediate cell-cell and cell-matrix interaction. (wikipedia.org)
  • Despite many years of effort, discovering the high-resolution structure of integrins proved to be challenging, as membrane proteins are classically difficult to purify, and as integrins are large, complex and highly glycosylated with many sugar 'trees' attached to them. (wikipedia.org)
  • Both cell types adhered to vitronectin by alpha v-containing integrins. (rupress.org)
  • Integrins are transmembrane proteins that facilitate cell-extracellular matrix interactions or focal adhesions. (bicellscientific.com)
  • Keratinocytes adhered to collagen with alpha 2 beta 1, but an antibody to alpha 2 did not inhibit adhesion of ndk to collagen. (rupress.org)
  • Here, we define the domain organization of Vn, report the crystal structure of its carboxyl-terminal domain, and show that it harbors the binding site for the Yersinia pestis outer membrane protein Ail, which recruits Vn to the bacterial cell surface to evade human host defenses. (nih.gov)
  • Harvey, H. Schlech III, and R. Immunobiology of gonococcal outer membrane protein I. Swanson, J. (levinguitars.eu)
  • 29. Purified alpha 2-macroglobulin receptor/LDL receptor-related protein binds urokinase.plasminogen activator inhibitor type-1 complex. (nih.gov)
  • Presently, DHF is established by assessing antibody concentrations and other rule-of-thumb criteria, but often these assays can be difficult to interpret and lead to false conclusions. (nih.gov)
  • A standard curve is generated using known amounts of antibodies while the unknown sample concentrations are extrapolated from the standard curve. (heelzup.com)
  • Membranes have been blocked with 5% nonfat milk in 1 TBS Tween 20, followed by incubation selleck chemical with mouse monoclonal anti human EDA FN antibody, rabbit polyclonal anti human FN antibody, rabbit polyclonal anti ERa antibody, rabbit polyclonal anti ERb antibody, mouse monoclonal anti human vitronectin, mouse monoclonal anti b actin, or mouse monoclonal anti GAPDH in one TBS Tween twenty. (plcsignaling.com)
  • This donor-selected human polyclonal immune globulin preparation contains high titers of naturally occurring antibodies against S. aureus ClfA and S. epidermidis SdrG. (medscape.com)
  • This assay can be implemented to rapidly detect and distinguish norovirus strains from genogroups I and II, which are responsible for the majority of human infections. (nih.gov)
  • CDC researchers have developed a RT-PCR multiplex assay that, prior to sero-conversion, selectively detects dengue virus in biological or other fluid media, such as whole blood, plasma, or serum. (nih.gov)
  • The concentration of the antibodies is then assessed with the direct binding assay reliably and reproducibly. (heelzup.com)
  • Interactions were tested in a solid phase assay, which led to the identification of 23 protein-protein interactions among which 20 are new. (biomedcentral.com)
  • Human IgG antibody Laboratories manufactures the protein assay reagent a #5000113 reagents distributed by Genprice. (fgf-erk.com)
  • The Protein Assay Reagent A #5000113 reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (fgf-erk.com)
  • PAI-1 plays a dual role in the regulation of cell migration through differential interactions with its binding partners such as uPA, tPA, vitronectin, and low-density lipoprotein receptor-related protein (LRP)1. (biomedcentral.com)
  • Dr. Schuck obtained his Ph.D. from the Goethe-University Frankfurt am Main, Germany, where he worked on interactions of integral proteins of the erythrocyte membrane using analytical ultracentrifugation. (nih.gov)
  • He received his post-doctoral research training in physical biochemistry with Dr. Allen Minton at NIDDK, and joined the Bioengineering and Physical Science Program of NCRR as a Research Fellow in 1997, developing biophysical methods for the study of protein interactions. (nih.gov)
  • The Laoratory of Dynamics of Macromolecular Assembly develops biophysical methods to study protein interactions and the assembly of multi-protein complexes. (nih.gov)
  • Hallmarks of multi-protein complexes are multi-valent interactions and cooperativity. (nih.gov)
  • We designed a medium throughput approach to systematically test for interactions between these pneumococcal surface proteins and host proteins (extracellular matrix proteins, circulating proteins or immunity related proteins). (biomedcentral.com)
  • Sedimentation velocity (SV) analytical ultracentrifugation is a highly versatile method that can shed light on many aspects of protein interactions, including the number of different classes of complexes, their stoichiometry, binding constants from the nanomolar to millimolar range, and structural changes associated with binding. (nih.gov)
  • The latter aspects highlight why SV is uniquely suited for the study of non-covalent protein interactions. (nih.gov)
  • The α and β subunits are both class I transmembrane proteins, so each penetrates the plasma membrane once, and can possess several cytoplasmic domains. (wikipedia.org)
  • TF is an integral transmembrane protein expressed by various cells, is a component of the factor VIIa-TF complex enzyme and is essential for normal hemostasis [ 1 , 2 ]. (hindawi.com)
  • A fluid occurring in minute amounts in the gingival crevice, believed by some authorities to be an inflammatory exudate and by others to cleanse material from the crevice, containing sticky plasma proteins which improve adhesions of the epithelial attachment, have antimicrobial properties, and exert antibody activity. (lookformedical.com)
  • The MSCRAMMs/surface proteins discussed now are targets for vaccine development. (medscape.com)
  • Pneumococcal surface proteins represent challenging candidates for the development of new therapeutic targets against the bacteria. (biomedcentral.com)
  • Availability of genome sequences facilitated the identification of pneumococcal surface proteins bearing characteristic motifs such as choline-binding proteins (Cbp) and peptidoglycan binding (LPXTG) proteins. (biomedcentral.com)
  • We cloned, expressed and purified 28 pneumococcal surface proteins. (biomedcentral.com)
  • It now appears clearly that cell-surface proteins participate in many stages of the colonization process and/or the disease transition. (biomedcentral.com)
  • PAI-1 promoted the migration of microglial cells in culture via the low-density lipoprotein receptor-related protein (LRP) 1/Janus kinase (JAK)/signal transducer and activator of transcription (STAT)1 axis. (biomedcentral.com)
  • Alveolar macrophages and alveolar epithelial cells are directly stimulated by bacterial endotoxins or indirectly by pro-inflammatory mediators ( e.g. tumour necrosis factor (TNF)-α) to produce pro-coagulant and anti-fibrinolytic proteins. (ersjournals.com)
  • 34. A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking. (nih.gov)
  • The CoMoFarm project was conceived to facilitate the development of high-yielding production systems based on plants, plant tissues and plant cells for the large-scale production of pharmaceutical and industrial proteins. (europa.eu)
  • The concept behind the project was to develop ways to standardize the growth and behavior of plants (and plant tissues and cells) so that pharmaceutical proteins can be produced to a consistent yield and quality. (europa.eu)
  • Endothelial cells may allow the transport of proteins and even cells through their cell body in what has been called the transcellular pathway. (hindawi.com)
  • Given that FUS +MB achieves BBB opening in part by disruption of tight junction (TJ) proteins such as claudin-5 of brain endothelial cells, we generated a stable MDCK (Madin-Darby Canine Kidney) II cell line (eGFP-hCldn5-MDCK II) that expresses fluorescently tagged human claudin-5. (thno.org)
  • conducted a series of experiments to identify specific proteins in the melanoma cellsthat were responsible for making the cancer more invasive after it interacted with the lymph vessel cells. (elifesciences.org)
  • Despite the relative simplicity and small size of TF, there are numerous contradictory reports about the synthesis and presentation of TF on blood cells and circulation in normal blood either on microparticles or as a soluble protein. (hindawi.com)
  • This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. (fgf-erk.com)
  • Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. (fgf-erk.com)
  • High-performance liquid chromatography (HPLC) has been used as the gold standard for antibody quantitation and purification for the past 50 years. (heelzup.com)
  • The system can perform protein quantitation in less than two minutes on a maximum of 96 samples. (heelzup.com)
  • Proteome characterization of the two acellular matrices showed enrichment of matrisome proteins and major cardiac extracellular matrix components, considerably higher for the recellularized pericardium. (nature.com)
  • 24. Vitronectin concentrates proteolytic activity on the cell surface and extracellular matrix by trapping soluble urokinase receptor-urokinase complexes. (nih.gov)
  • These diseases have in common the increased reactive oxygen species (ROS), mitochondrial dysfunctions, protein misfolding and aggregation, neuroinflammation, progressive loss of specific subtypes of neurons, and so far, the lack of a cure. (springer.com)
  • We conclude that whether peptidoglycan binding proteins do not appear to be major adhesins, most of the choline-binding proteins interact with host proteins (elastin and C reactive proteins are the major Cbp partners). (biomedcentral.com)
  • Another drawback is its inability to derive binding kinetics to determine antibody performance, i.e., affinity and dissociation constants. (heelzup.com)
  • Although not a very informative method for determining detailed binding kinetics, it can be readily detected whether the kinetics is fast or slow relative to the time-scale of sedimentation (103-104 sec). (nih.gov)
  • The incubation with anti-GAPDH antibody significantly decreased the adherence of M. hyorhinis to both cell lines. (biomedcentral.com)
  • The data suggest that pre incubation with clinically suitable binders to TJ proteins may be a general strategy to facilitate safer and more effective ultrasound-mediated BBB opening in cellular and animal systems and potentially also for the treatment of human diseases of the brain. (thno.org)
  • This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
  • Among them, the enzymes in the glycolysis pathway are the most common proteins. (biomedcentral.com)
  • In this pathway, fluid and proteins are actively transported in a complex system of vesicles from the luminal to the basal side of the cell, where the vesicular content is released [ 13 , 17 ]. (hindawi.com)
  • Complement component 1q (C1q) is the recognition molecule of the classical pathway of the complement system that can bind to an array of closely spaced antigen-bound immunoglobulin G (IgG) and IgM antibodies. (explorationpub.com)
  • ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
  • The levels of PAI-1 mRNA and protein expression were increased by lipopolysaccharide and interferon-γ stimulation in both microglia and astrocytes. (biomedcentral.com)
  • Diversity of Short Linear Interaction Motifs in SARS-CoV-2 Nucleocapsid Protein. (nih.gov)
  • N-linked protein glycosylation (N-glycosylation of N-glycans) at Asn residues (Asn-x-Ser/Thr motifs) in glycoproteins [1] . (wikidoc.org)
  • The algU gene encodes a protein homologous to an alternative sigma factor regulating sporulation and other developmental processes in Bacillus, and along with the negative regulators mucA and mucB comprises the gene cluster controlling conversion to mucoidy. (justia.com)
  • The PTK2 gene encodes a cytosolic protein tyrosine kinase. (faksignaling.com)
  • M. hyorhinis has been detected in cancerous tissues from gastric, esophageal, lung, breast, glioma and colon cancers by polymerase chain reaction (PCR) or immunohistochemical staining (IHC) [ 2 ]. (biomedcentral.com)
  • Tissues samples from 154 cases of ovarian carcinoma were immunostained with anti-PAI-1 antibody, and the prognostic value was analyzed. (oncotarget.com)
  • One of 10 patients had an EGFR-positive tumor detected by both microarray and immunohistochemistry. (nih.gov)
  • One tumor kinases such as insulin-like growth factor I receptor (IGFIR) and expressed significant levels of EGFR protein and erlotinib had insulin receptor (1, 2). (nih.gov)
  • The image below depicts positive staining of tumor cell nuclei with monoclonal antibody to estrogen receptor. (medscape.com)
  • Somatic mutations vary in distinct cancer types (eg, brain, pancreas, breast, colon), as well as in a given tumor type, but they do appear to utilize common overlapping oncogenic pathways detected in the malignant phenotype. (medscape.com)
  • Complementary to crystallographic techniques, such solution interaction studies can provide information on the assembly principles of structurally polymorph multi-protein complexes. (nih.gov)
  • 31. alpha-2 Macroglobulin receptor/Ldl receptor-related protein(Lrp)-dependent internalization of the urokinase receptor. (nih.gov)
  • 36. Direct binding of occupied urokinase receptor (uPAR) to LDL receptor-related protein is required for endocytosis of uPAR and regulation of cell surface urokinase activity. (nih.gov)
  • No cleavage of PAI-1 was detected with Yersinia pseudotuberculosis, an oral/fecal pathogen from which Y. pestis has evolved, or with Escherichia coli. (nih.gov)
  • Cleavage of PAI-1 was also detected with the omptins Epo of Erwinia pyrifoliae and Kop of Klebsiella pneumoniae, which both belong to the same omptin subfamily as Pla and PgtE, whereas no cleavage of PAI-1 was detected with omptins of Shigella flexneri or E. coli or the Yersinia chromosomal omptins, which belong to other omptin subfamilies. (nih.gov)
  • We are involved in several collaborative applications in various fields including immunological protein complexes, viral proteins, membrane receptor complexes, and eye lens crystallins. (nih.gov)
  • In this study, we investigated the aberrant changes in N-glycosylation pattern of proteins associated with tumorigenesis as well as disease progression in bladder cancer. (oncotarget.com)
  • Moreover, we identified altered glycosylation in 41 NMIBC and 21 MIBC proteins without any significant change in protein abundance levels. (oncotarget.com)
  • In concordance with the previously published bladder cancer cell line N-glycoproteomic data, we also observed dysregulated glycosylation in ECM related proteins. (oncotarget.com)
  • PAI-1 inhibited microglial engulfment of zymosan particles in a vitronectin- and Toll-like receptor 2/6-dependent manner. (biomedcentral.com)
  • Panel A. The mCherry Monoclonal Antibody recognizes mCherry but not tdTomato, DsRed-Express2, or DsRed-Monomer. (takarabio.com)
  • Although disputed, SdrG could not be detected at the bacterial surface in several in vitro conditions, but was expressed at the surface within 1 h after injection in the bloodstream of a mouse, indicating that sdrG transcription is induced in response to host-specific signals in invasive infection and that it may not be transcribed during commensal life. (medscape.com)
  • [ 75 ] Using SdrG-deficient mutants or opsonic anti-SdrG antibodies, the role of SdrG in biofilm formation and virulence was clearly demonstrated. (medscape.com)
  • Over the past 20 years, the importance of proteins for S. pneumoniae virulence has become clear. (biomedcentral.com)
  • The data interpretation is based on first principles and can give absolute protein molecular weights and hydrodynamic translational frictional coefficients (leading to low resolution shape information of macromolecules in solution). (nih.gov)
  • They have shown vitronectin is significantly reduced in DHF and severe dengue infections when compared to dengue non-hemorrhagic fever patients. (nih.gov)
  • [ 83 ] This lack of antibody therapy efficacy may be due to an insufficient clearance of the pathogens resulting from deficiencies in the innate immune response in neonates. (medscape.com)
  • Erlotinib has been tested in various phases of clinical trials as shown by the decreased activities of phosphorylated-EGFR, for its safety, tolerability, and treatment efficacy with the most phosphorylated-mitogen-activated protein kinase, and phos- activity seen in non - small cell lung cancer. (nih.gov)
  • The sugar group(s) can assist in protein folding or improve proteins' stability. (wikidoc.org)
  • Resultant shifts in electrophoretic migration help distinguish among proteins with N-glycan, O-glycan, or GPI linkages and also between high mannose and complex N-glycans. (wikidoc.org)
  • Tauopathies are a class of neurodegenerative diseases that are characterized by the progressive aggregation of tau protein and include Alzheimer's Disease (AD) and frontotemporal dementias (FTD) such as Pick's Disease, Corticobasal Degeneration and Progressive Supranuclear Palsy. (biomedcentral.com)
  • The resulting sedimentation boundaries show strongly size-dependent separation of protein species. (nih.gov)
  • In contrast, for example, to conventional size-exclusion chromatography, the faster migrating protein complexes will always remain in a bath of the slower sedimenting species, such that association and dissociation events from reversible complexes will continue to take place throughout the experiment, maintaining reversible complexes in a way that reflects the chemical equilibria and kinetic properties of the interaction. (nih.gov)
  • The detection antibody is labeled and requires another step to detect the presence of the label which correlates to the quantity of the biomolecule of interest. (heelzup.com)
  • Basically, glycoprotein is a biomolecule composed of a protein and a carbohydrate (an oligosaccharide). (wikidoc.org)
  • Vitronectin (Vn) is a major component of blood that controls many processes central to human biology. (nih.gov)
  • The structure explains key features of native Vn and provides a blueprint for understanding and targeting this essential human protein. (nih.gov)
  • sub.2B -adrenergic receptor, and an isolated protein which is a human .alpha. (justia.com)
  • The invention herein also concern an antibody directed to the human .alpha. (justia.com)
  • The Worldwide Society of Urologic Pathologists (ISUP) grade 5 and pT3b as a pathological stage have been detected as impartial variables within the prediction of BCR formation. (milansystem.org)
  • Two identified groups of genes with differential expression were those encoding for ribosomal proteins and attachment proteins, such as the RGD (Arg-Gly-Asp)-associated proteins. (benthamopen.com)
  • This study demonstrates that MFG-hESC lines overexpress cell attachment proteins but retain the typical characteristics of undifferentiated feeder-cultured hESCs. (benthamopen.com)
  • Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
  • Gene changesin EGFR-negative tumorsare those of G-protein-linked and cell surface receptor ^ linked signaling. (nih.gov)
  • Among 495 gene ontology categories, the significant dif- fered gene ontology groupsinclude G-protein-coupled receptor protein signaling (34 genes, P = 0.002) and cell surface receptor ^ linked signal transduction (74 genes, P = 0.007). (nih.gov)
  • If a genetic variant is relevant in only some subtypes (defined by sex, erosion status, auto-antibodies, or other parameters), its effect may be present in one cohort but not in another and may appear with smaller effect size in large, multi-center cohorts. (biomedcentral.com)
  • Tissue factor (TF) is an integral membrane protein that is essential to life. (hindawi.com)
  • Macro and micromechanics, structural characteristics and protein content of the decellularized scaffolds were evaluated prior to recellularization. (nature.com)
  • In addition to its multiple procoagulant roles, thrombin also acts as an anticoagulant when combined with the cofactor thrombomodulin in the protein Case complex. (hindawi.com)
  • Nevertheless, due to restricted information concerning the period of humoral immunity to COVID-19 and the existence of distinctive particular person immune responses, the potential position of antibody testing within the analysis of present and previous infections of COVID-19 stays ambiguous. (milansystem.org)