Dendritic cells derivedMature dendritic cellsCarcinoembryonic antigenImmunodominantAllelesCytotoxicInduceAutologousImmunotherapyMelanomaEpitopeAllogeneicLymphocytesTransgenicTumor cellsVivoAdjuvantsVaccinationTumorsResponsesLigandGenerateVaccineProteinCellsHumanPotentiallyAntibodiesPatientsSpecificClassHighly
Dendritic cells derived2
- Autologous mature dendritic cells derived from peripheral blood monocytes were pulsed with the HLA-A2-binding MUC1 peptides (M1.1 and M1.2). (aacrjournals.org)
- Banchereau J, Ueno H, Dhodapkar M, Connolly J, Finholt JP, Klechevsky E, Blanck JP, Johnston DA, Palucka AK, Fay J. Immune and clinical outcomes in patients with stage IV melanoma vaccinated with peptide-pulsed dendritic cells derived from CD34+ progenitors and activated with type I interferon. (nwbio.com)
Mature dendritic cells1
- Bleumer I, Tiemessen DM, Oosterwijk-Wakka JC, Völler MC, De Weijer K, Mulders PF, Oosterwijk E. Preliminary analysis of patients with progressive renal cell carcinoma vaccinated with CA9-peptide-pulsed mature dendritic cells. (nwbio.com)
Carcinoembryonic antigen1
- Babatz J, Röllig C, Löbel B, Folprecht G, Haack M, Günther H, Köhne CH, Ehninger G, Schmitz M, Bornhäuser M. Induction of cellular immune responses against carcinoembryonic antigen in patients with metastatic tumors after vaccination with altered peptide ligand-loaded dendritic cells. (nwbio.com)
Immunodominant1
- The crystal structure of human and murine T cell receptors (TcRs) specific for immunodominant viral peptides presented to cytotoxic T cell (CTL) suggests that the central region of the peptide is critical for TcR contacts and that aromatic rings are accommodated in a small cavity determined by the variable CDR3 region of the TcR ( 7 , 8 ). (silverchair.com)
Alleles2
- In addition, it is likely that protein vaccination leads to presentation of epitopes in the context of various HLA alleles, and therefore this type of vaccine should be applicable to any patient regardless of HLA restriction. (wikipedia.org)
- Peptides were synthesized and tested for in vivo immunogenicity by screening peripheral blood mononuclear cells from healthy volunteers (n = 64) and HAdV-infected stem cell recipients (n = 26) for memory T cells recognizing the candidate epitopes in the context of most common HLA alleles. (biomedcentral.com)
Cytotoxic5
- This is due to the fact that most tumors are positive for MHC class I but negative for MHC class II, and CD8+ cytotoxic T lymphocytes (CTLs) are able to induce tumor killing upon direct recognition of peptide antigens presented by the tumor's MHC class I molecules. (wikipedia.org)
- Vaccination with tumor lysate-pulsed dendritic cells elicits antigen-specific, cytotoxic T-cells in patients with malignant glioma. (nwbio.com)
- Immature dendritic cells phagocytose apoptotic cells via alphavbeta5 and CD36, and cross-present antigens to cytotoxic T lymphocytes. (nwbio.com)
- Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (thermofisher.com)
- Outcomes for patients with melanoma have improved over the past decade with the clinical development and approval of immunotherapies targeting immune checkpoint receptors such as programmed death-1 (PD-1), programmed death ligand 1 (PD-L1) or cytotoxic T lymphocyte antigen-4 (CTLA-4). (biomedcentral.com)
Induce5
- We aimed to clarify whether soluble CD40 ligand (sCD40L) activated B cells may be loaded with HBcAg18-27 peptide and served as antigen-producing cells (APCs) to induce HBV-specific cytolytic T lymphocytes (CTLs). (biomedcentral.com)
- Furthermore, the peptide-pulsed activated B cells could induce HBcAg18-27 specific CTLs. (biomedcentral.com)
- Dendritic cells acquire antigen from apoptotic cells and induce class I-restricted CTLs. (nwbio.com)
- In the present study we have analyzed 10 potential 9-mer epitopes containing the HLA-A2.1 binding motifs for their ability to induce melanoma-specific T cell lines. (elsevierpure.com)
- We conducted a pilot randomized phase I trial in PC patients to determine the peptide dose required to induce an optimal CD8 + T cell response. (bmj.com)
Autologous3
- Vaccination of prostatectomized prostate cancer patients in biochemical relapse, with autologous dendritic cells pulsed with recombinant human PSA. (nwbio.com)
- MART-1 27-35 -specific CTL could be effectively generated from a total of 11 of 12 PBL and from 3 of 3 TIL derived from HLA-A2 + melanoma patients, as well as from 2 of 4 PBL from HLA-A2 + healthy donors by in vitro stimulation with autologous PBMC pulsed with the synthetic MART-1 27-35 peptide. (elsevierpure.com)
- Autologous as well as allogeneic CD8 + T cells transduced with tumor antigen specific T cell receptors (TCR) may cause significant tumor lysis upon adoptive transfer. (oncotarget.com)
Immunotherapy2
- These results demonstrate that MART-1 27-35 peptide may represent an ideal candidate for Ag-specific immunotherapy in melanoma patients. (elsevierpure.com)
- Because of these encouraging results, we applied the same antigenic strategy to TACA-containing glycopeptides because induction of a degenerate T cell response against this class of tumor antigens may be of great advantage in cancer immunotherapy ( 10 - 12 ). (silverchair.com)
Melanoma3
- MART-1 is an Ag expressed on melanomas and melanocytes, and is recognized by the majority of HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes (TIL) from melanoma patients. (elsevierpure.com)
- These CTL lines specifically lysed and release cytokines (TNF-α, IFN-γ, and GM-CSF) in response to T2 cells pulsed with MART-1 27-35 , as well as to HLA-A2 + MART-1 + melanoma cells. (elsevierpure.com)
- CTL generated with MART-1 27-35 also lysed uncultured HLA-A2 + melanoma cells derived from tumor biopsies, indicating that this MART-1 epitope is likely to be expressed in association with HLA-A2 on the surface of tumor cells in vivo. (elsevierpure.com)
Epitope1
- The inclusion of CD4+ epitopes into MAGE-3 vaccination studies has recently been facilitated by the identification of several HLA-DR-restricted and one HLA-DP4-restricted epitope. (wikipedia.org)
Allogeneic3
- Allogeneic-donor-derived cells can be genetically modified to eliminate expression of HLA-A. (ashpublications.org)
- HLA-A disruption from donor cells is a step toward generating allogeneic cells as an off-the-shelf therapeutic. (ashpublications.org)
- Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)
Lymphocytes2
- For the activation of CD4 + T-helper lymphocytes, dendritic cells were further incubated with the PAN-DR-binding peptide PADRE. (aacrjournals.org)
- Furthermore, we provide evidence that the activated B cells may serve as APCs to present core peptide of HBV to cytolytic T lymphocytes (CTLs). (biomedcentral.com)
Transgenic6
- Methods Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (bmj.com)
- Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)
- After TCR identification, we generated HLA-A*02:01/peptide restricted TCR transgenic T cells by retroviral transduction and tested T cell expansion rates as well as A*02:01/peptide recognition and ES killing in ELISpot and xCELLigence assays. (oncotarget.com)
- TCR transgenic T cells demonstrated HLA-A*02:01/ADRB3 295 mediated ES recognition and killing in ELISpot and xCELLigence assays. (oncotarget.com)
- Amino-acid exchange scans clearly indicated the cross-reactive potential of HLA-A*02:01/ADRB3 295 - and HLA-A*02:01/CHM1 319 -TCR transgenic T cells. (oncotarget.com)
- Comparison of peptide motive binding affinities revealed extended fratricide among ADRB3 295 specific TCR transgenic T cells in contrast to CHM1 319 . (oncotarget.com)
Tumor cells1
- In the current study we show that designer glycopeptides containing the Thomsen-Freidenreich (TF) antigen (β-Gal-[1→3]-α-GalNAc- O -serine) are immunogenic in vivo and generate TF-specific CTL capable of recognizing a variety of tumor cells in vitro including a MUC1-expressing tumor. (silverchair.com)
Vivo3
- CD8 responses were assessed in vitro for cytotoxicity and in vivo tumor therapy. (bmj.com)
- Results Homocitrullinated peptides from aldolase and cytokeratin were identified, that stimulated CD8-mediated responses in vivo. (bmj.com)
- Efficient antigen presentation by antigen presenting cells (APCs) is critical for inducing T-cell mediated immunity in vivo [ 1 , 2 ]. (biomedcentral.com)
Adjuvants1
- Further evaluation of 1 mg CEA peptide with stronger adjuvants, and/or combined with agents to overcome immune inhibitory pathways, may be warranted in PC pts. (bmj.com)
Vaccination3
- citation needed] Clinical vaccination studies using full-length recombinant proteins have the advantage that this form of antigen potentially includes the full range of epitopes for CD4+ and CD8+ T T cells. (wikipedia.org)
- Vaccination resulted in robust spike-specific T cell responses for the dominant CD4+ (HLA-DRB1∗15:01/S191) and CD8+ (HLA-A∗02/S691) T cell epitopes. (stanford.edu)
- Antigen-specific CD4+ and CD8+ T cell responses were asynchronous, with the peak CD4+ T cell responses occurring 1 week post the second vaccination (boost), whereas CD8+ T cells peaked 2 weeks later. (stanford.edu)
Tumors1
- Tumors express embryonic carbohydrate antigens called tumor-associated carbohydrate antigens (TACA). (silverchair.com)
Responses5
- Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)
- Even though CD4+ T cells are known to play a central role in regulating virtually all antigen-specific immune responses, the role they play in immune responses to tumor antigens has not been widely studied compared to that of CD8+ T cells. (wikipedia.org)
- Antigen-specific CD40L-activated B cells may efficiently endocytose and present antigens, such as protein, RNA, and cDNA, to prime primary T cells and boost robust memory T-cell responses [ 9 ]. (biomedcentral.com)
- Thus, the local immunological milieu in tissue compartments forms the nature and magnitude of the host responses, i.e., frequencies of regulatory T cells (Tregs) vs. T-helper 17 (Th17) cells, amount of pro-inflammatory cytokines vs. anti-inflammatory cytokines, extent of neutrophilia and antigen-presenting-cell (APC) activation, among others. (frontiersin.org)
- Conclusions The T cell response in this randomized phase I trial was dose-dependent with the 1 mg CEA peptide dose eliciting the most robust T cell responses. (bmj.com)
Ligand1
- CAP1-6D, an altered peptide ligand can help bypass tolerance. (bmj.com)
Generate1
- computer simulations of proteolysis of marburg and ebola-zaire filovirus coded proteins to generate nonapeptides with motifs of known hla class i haplotypes and detection of antigenic domains in the viral glycoproteins. (liverpool.ac.uk)
Vaccine1
- Using "spheromer" peptide-MHC multimer reagents, we analyzed healthy subjects receiving two doses of the Pfizer/BioNTech BNT162b2 vaccine. (stanford.edu)
Protein1
- citation needed] In contrast, of eight patients from the second cohort vaccinated with MAGE-3 protein and adjuvant, seven developed high-titered antibodies to MAGE-3, and four had a strong concomitant CD4+ T cell response to HLA-DP4-restricted peptide 243-258. (wikipedia.org)
Cells10
- CD4+ T cells promote anti-tumor immunity through numerous mechanisms, including enhancing antigen presentation, co-stimulation, T cell homing, T cell activation, and effector function. (wikipedia.org)
- furthermore, recent reports suggest that immunization (using either adjuvant or dendritic cells with pure tumor peptides) can result in productive anti-tumor immunity that is restricted by MHC class I. Finally, elimination of CD8+ T cells from mice at least partially abrogates anti-tumor immunity induced by most cancer vaccines. (wikipedia.org)
- One patient simultaneously developed CD8+ T cells to HLA-A1-restricted peptide 168-176. (wikipedia.org)
- Cytometry and fluorescence microscopy showed that more than 98% sCD40L-activated B cells were loaded by the HBcAg peptide. (biomedcentral.com)
- Dendritic cells (DCs), activated macrophages, and activated B cells are all capable of presenting antigen peptides. (biomedcentral.com)
- However, little is known whether CD40L-activated B cells may present HBV antigen to T cells. (biomedcentral.com)
- In this study, we set up an effective culture method for long-term maintenance of B cells in vitro , in which the B cells are activated by human soluble CD40L (sCD40L). (biomedcentral.com)
- We identified HLA-A*02:01/peptide-restricted T cells directed against ADRB3 295 . (oncotarget.com)
- HLA-identical sibling stem cells are used for the treatment of patients suffering from various hematologic malignant and nonmalignant diseases. (ashpublications.org)
- Functional CD8 + T cells recognizing seven epitopes were identified, among them four penton-derived and two hexon-derived peptides. (biomedcentral.com)
Human3
- Human tumor samples were analyzed by targeted mass spectrometry for presence of homocitrullinated peptides. (bmj.com)
- Figure B. FITC conjugated Mouse anti Human CD19 ( MCA1940F ) and Pacific Blue conjugated Mouse anti Human HLA ABC ( MCA81PB ). (bio-rad-antibodies.com)
- Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 ( MCA1940A488 ) and RPE-Alexa Fluor 647 conjugated Mouse anti Human HLA ABC ( MCA81P647 ). (bio-rad-antibodies.com)
Potentially1
- Amino-acid exchange scans alone predict TCR cross-reactivity with little specificity and thus require additional assessment of potentially cross-reactive HLA-A*02:01 binding candidates. (oncotarget.com)
Antibodies1
- Antibodies to gp350 neutralize pathogen in vitro [1]. (exatecan-mesylate.com)
Patients2
- Of nine patients in the first cohort, three developed marginal antibody titers and another one had a CD8+ T cell response to HLA-A2-restricted peptide MAGE-3 271-279. (wikipedia.org)
- Methods Patients with a PS 0-1, HLA-A2+ and CEA-expressing, previously-treated PC were randomized to receive 10 μg (arm A), 100 μg (arm B) or 1000 μg (arm C) of CEA peptide emulsified in Montanide and GM-CSF, given every 2 weeks until disease progression. (bmj.com)
Specific2
- In fact, our preliminary studies using trinitrophenyl (TNP) as a hapten model, suggested that K b -restricted peptides haptenated in position 4 in 8 mers or position 5 in 9 mers induced a TNP-specific CTL repertoire whose TcR was poorly dependent on MHC class I contacts and highly degenerate ( 9 ). (silverchair.com)
- Forty-two of 104 tetramer pos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. (ashpublications.org)
Class3
- the primary amino acid sequences of the proteins coded by marburg and ebola-zaire filoviruses were studied by computer programs to search for putative proteolytic cleavages which yield nonapeptides with motifs of binding to known hla class i haplotypes. (liverpool.ac.uk)
- the computer analyses predicted that numerous nonapeptides with motifs to bind hla class i a68 and a2 haplotypes were detected. (liverpool.ac.uk)
- a few nonapeptides with motifs hla class i a24, b8, b27 and b35 were predicted in marburg virus proteins. (liverpool.ac.uk)
Highly1
- it permits a highly accurate and sensitive rapid detection of the antigen. (liverpool.ac.uk)