• Embryo cryopreservation is useful for leftover embryos after a cycle of in vitro fertilisation, as patients who fail to conceive may become pregnant using such embryos without having to go through a full IVF cycle. (wikipedia.org)
  • Spare oocytes or embryos resulting from fertility treatments may be used for oocyte donation or embryo donation to another woman or couple, and embryos may be created, frozen and stored specifically for transfer and donation by using donor eggs and sperm. (wikipedia.org)
  • Oocyte age, survival proportion, and number of transferred embryos are predictors of pregnancy outcome. (wikipedia.org)
  • A study of more than 11,000 cryopreserved human embryos showed no significant effect of storage time on post-thaw survival for IVF or oocyte donation cycles, or for embryos frozen at the pronuclear or cleavage stages. (wikipedia.org)
  • In 2021, she began her doctoral studies, under Dr. Charles Love, focused on the study of various clinical factors associated with the efficiency of assisted reproductive technologies in the mare, particularly Intracytoplasmic Sperm Injection (ICSI) and in vitro culture of equine embryos. (tamu.edu)
  • Various of these studies are conducted in collaboration with Dr. Terje Raudsepp, including the validation of alternative methods of preimplantation genetic diagnosis in embryos, and the impact of ICSI and in vitro culture on the genome and transcriptome of equine embryos. (tamu.edu)
  • She also has interests in the application of molecular biology techniques for gametes and embryos, and the relationship of these to clinical equine reproduction. (tamu.edu)
  • Embryos produced outside the breeding season can be frozen for later transfer, which makes these techniques very versatile. (ivis.org)
  • However, other procedures based on the in vivo and in vitro production of equine embryos have emerged in recent years. (ivis.org)
  • Reasons for the delayed development of in vivo and in vitro production of equine embryos include the scarce availability of abattoir ovaries and the lack of interest from horse breeders and registries. (ivis.org)
  • To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
  • The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
  • ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
  • Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
  • Oocytes, sperms, embryos and testicular tissue can be cryopreserved and can later be used for conception. (oasisindia.in)
  • In this technique women's eggs are fertilized with the sperm of the husband and then frozen, so that the embryos can be used when the couple is ready to have a baby. (oasisindia.in)
  • Retrospectively, it seems absurd, but the respected big-bearded academics disregarded the obvious fact that - unlike those of frogs and salamanders - oocytes and embryos of mammals are extremely sensitive to temperature changes and do not tolerate exposition to room temperature, not even for a short period. (vitavitro.com)
  • Along with polyspermy, the suboptimal quality of IVF-derived pig embryos poses another challenge that needs to be addressed for in vitro production systems. (e-jarb.org)
  • With logical monitoring during the stimulation, we can prevent ovarian hyperstimulation syndrome using the cancellation of the cycle, coasting, triggering oocytes maturation with GnRH agonists in protocols with GnRH antagonists, cabergoline treatment and vitrification all embryos. (amedi.sk)
  • The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. (wakehealth.edu)
  • In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO. (wakehealth.edu)
  • Preimplantation genetic testing (PGT) is a technique used to identify chromosomal genetic abnormalities in embryos created through in vitro fertilization (IVF) before pregnancy. (medscape.com)
  • Successful implantation requires good quality of embryos, receptive endometrium, and proper embryo transfer technique" [1]. (peertechzpublications.com)
  • Influence of organochlorine pesticides on development of mouse embryos in vitro . (cdc.gov)
  • Assisted reproductive techniques (ARTs) involve manipulation of sperm and ova or embryos in vitro with the goal of producing a pregnancy. (msdmanuals.com)
  • The purpose of this study was therefore to compare treatment of P with DHP on batch level, in vitro, to determine dose-response effects, and in vivo, at a single dose. (wur.nl)
  • Our results support the conclusion that DHP and P work equally well in vitro and in vivo. (wur.nl)
  • Today, the most practical use of OPU or transabdominal follicle aspiration is to recover in vivo matured oocytes [ 9 ] for oocyte transfer. (ivis.org)
  • This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
  • We have recently established a novel culture system in which mouse pluripotent stem cells differentiate to mature oocytes in a manner similar to oogenesis in vivo. (researchsquare.com)
  • The long term goal of this work is to establish whether chemicals which are identified on the basis of in vitro bioactivity are predictive of effects in vivo and to determine which endocrine responses are most sensitive in terms of whole animal and population levels effects. (uoguelph.ca)
  • Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
  • In vitro maturation (IVM) of oocytes retrieved at germinal vesicle stage, followed by vitrification of mature oocytes, has emerged as a fertility preservation (FP) option. (amsterdamumc.org)
  • This protocol consists of a culture method that can be mainly divided into four periods: primordial germ cell-like cell (PGCLC) derivation, primary oocyte differentiation (in vitro differentiation: IVDi), oocyte growth to germinal vesicle (GV) oocytes (in vitro growth: IVG) and oocyte maturation (in vitro maturation: IVM). (researchsquare.com)
  • Assisted reproductive technologies (ARTs) are techniques to help a woman become pregnant, including in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and other similar procedures. (health.am)
  • At this point, intracytoplasmic sperm injection-injection of a single sperm into each oocyte-may be done, particularly if spermatogenesis is abnormal in the male partner. (msdmanuals.com)
  • ICSI produced the first pregnancy derived from an in vitro matured oocyte [ 5 ], and it was successfully carried to term. (ivis.org)
  • Mature oocytes area unit impregnated victimization of the ICSI technique . (ferty9.co.in)
  • The main techniques used for embryo cryopreservation are vitrification versus slow programmable freezing (SPF). (wikipedia.org)
  • The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. (jove.com)
  • Juvenile oocyte vitrification was a lot shorter in the generation interval in breeding programs. (jove.com)
  • In this video, we describe a protocol for the vitrification of sheep oocyte, collected from both juvenile and adult donors, and in vitro matured to prior to cryopreservation. (jove.com)
  • The protocol include all the procedures from in vitro maturation, vitrification, warming, and post-warming culture. (jove.com)
  • The selection of the oocytes to submit at the vitrification procedures represent the first important step to warranty the successful application of this procedure. (jove.com)
  • The loading of the oocyte in the vitrification device used is an important and critical step. (jove.com)
  • In this method, the oocytes in the vitrification solution, less than 0 point milliliter are rapidly loaded with a glass capillary on top of the film strip. (jove.com)
  • We offer Oocyte, Sperm and Embryo Vitrification along with Testicular Tissue Cryopreservation and In Vitro Maturation of Oocytes as part of the fertility preservation program. (oasisindia.in)
  • At OASIS we use a novel technique called VITRIFICATION to cryopreserve various reproductive tissues. (oasisindia.in)
  • Embryo cryopreservation is generally performed as a component of in vitro fertilization (which generally also includes ovarian hyperstimulation, egg retrieval and embryo transfer). (wikipedia.org)
  • Oocyte retrieval from donor. (lushfertility.in)
  • Donor egg program includes oocyte retrieval from healthy young oocyte. (lushfertility.in)
  • To assess if the absence of therapeutic intervention prior to oocyte retrieval for IVM modifies IVM outcomes in BC patients undergoing urgent FP, we performed a non-inferiority, randomized controlled trial. (amsterdamumc.org)
  • A total of 172 normo-ovulatory women, suffering from BC, 18 to 39 years of age received no injection or a subcutaneous injection of hCG or GnRH agonist (GnRHa) 36 h before oocytes retrieval according to randomized allocation. (amsterdamumc.org)
  • In G-CSF group (n=30), 300 µg trans-cervical intrauterine of G-CSF was administered at the oocyte retrieval day. (peertechzpublications.com)
  • pregnancy rates with this technique are lower than those with retrieval of multiple oocytes, but costs are lower and success rates are increasing. (msdmanuals.com)
  • There, she was trained in transvaginal oocyte aspiration, oocyte and embryo manipulation in the mare, and conducted studies on in vitro capacitation of stallion sperm. (tamu.edu)
  • In vitro fertilization (IVF) involves combining an egg and sperm within a laboratory setting to create an embryo. (ivf.net)
  • In vitro Fertilization of retrieved oocytes with recipient's husband's sperm /Donor sperm obtained from Sperm bank. (lushfertility.in)
  • But, with medical advancements, young men and women can opt for fertility preservation techniques where they can vitrify their sperm or oocytes to be used later. (oasisindia.in)
  • However these treatments are proven to either slow down the oocyte or sperm production or damage the reproductive organs leading to infertility in the patient. (oasisindia.in)
  • 4 Of note, PGCLCs gave rise to sperm and oocytes by transplantation into neonatal testes and adult ovaries, respectively, 4,5 which means that PGCLCs have the potential to give rise to functional gametes. (researchsquare.com)
  • In its simplest term, IVF is simply the uniting of egg and sperm in vitro (in the lab). (virascience.com)
  • in-vitro -matured oocytes were co-incubated with sperm according to a modified two-step culture system. (e-jarb.org)
  • In the first experiment, oocytes were briefly co-incubated with sperm, washed in IVF medium, and then moved to fresh IVF medium for 5 or 6 h. (e-jarb.org)
  • In experiment 2, oocytes were inseminated with sperm separated by Pecroll treatment. (e-jarb.org)
  • For assisted reproductive techniques, oocytes and sperm are collected from the intended parents or donors, and an embryo or the gametes are transferred to the woman's reproductive tract after culture in vitro. (msdmanuals.com)
  • The semen sample is typically washed several times with tissue culture medium and is concentrated for motile sperm, which are then added to the medium containing the oocytes. (msdmanuals.com)
  • After sperm are added, the oocytes are cultured for about 2 to 5 days. (msdmanuals.com)
  • The ovarian hyperstimulation is preferably done by using a GnRH agonist rather than human chorionic gonadotrophin (hCG) for final oocyte maturation, since it decreases the risk of ovarian hyperstimulation syndrome with no evidence of a difference in live birth rate (in contrast to fresh cycles where usage of GnRH agonist has a lower live birth rate). (wikipedia.org)
  • In addition, the duration of storage had no significant effect on clinical pregnancy, miscarriage, implantation, or live birth rate, whether from IVF or oocyte donation cycles. (wikipedia.org)
  • Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle -coordinating centrosomes . (wikipedia.org)
  • The use of preimplantation genetic testing for aneuploidy (PGT-A), formerly known as preimplantation genetic screening or PGS, has increased in recent years, now encompassing an estimated 40% of in vitro fertilization (IVF) cycles in the United States. (medscape.com)
  • Prior to the onset of menopause (10-15 y), menstrual cycles shorten and rapid follicular loss occurs because more oocytes are being recruited. (medscape.com)
  • This technique was first developed for patients with polycystic ovarian syndrome. (amsterdamumc.org)
  • The mammalian oocyte orchestrates the rate of ovarian follicular development. (yarsi.ac.id)
  • In the fifties of the last century, we had already the nuclear bomb, the sputnik, the television, the first digital computers - but not a single mammalian embryo produced in vitro . (vitavitro.com)
  • Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
  • The oocyte is the largest and most mysterious cell in the body of mammalian organisms. (ivf.net)
  • Here, we report an original case of GROS, associated with compound heterozygous follicle-stimulating hormone receptor (FSHR) variants, in a woman who achieved a live birth by in vitro maturation (IVM) of her oocytes. (hal.science)
  • This 31-year-old woman consulted our assisted reproduction center for a second opinion after having been advised, because of pervasive high serum follicle-stimulating hormone (FSH) levels, to pursue in vitro fertilization (IVF) with donor oocytes. (hal.science)
  • The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
  • Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro. (yarsi.ac.id)
  • Regulation of mouse follicle development by follicle stimulating hormone in a three-dimensional in vitro culture system is dependent on follicle stage and dose. (yarsi.ac.id)
  • Ovulation in European eel is induced by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the maturation-inducing hormone (MIH). (wur.nl)
  • Expression of genes involved in oocyte maturation and ovulation was similar in vitro for both DHP and P treatments. (wur.nl)
  • While our studies in the past used goldfish and rainbow trout our most recent work focuses on the zebrafish so that we can exploit the daily spawning in order to characterize the temporal sequence of endocrine changes that accompany oocyte final maturation and ovulation. (uoguelph.ca)
  • We study the control of growth, atresia and hormone biosynthetic capacity of zebrafish ovarian follicles during the period of oocyte maturation and at ovulation. (uoguelph.ca)
  • As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
  • After sufficient follicular growth, human chorionic gonadotropin (hCG) is given to trigger final follicular maturation and ovulation. (msdmanuals.com)
  • Vitrifaction by ultrarapid cooling and warming is considered a standard approach in cattle oocyte preservation. (jove.com)
  • Decline in the Number of Oocytes from Birth to Menopause. (ldysinger.com)
  • The decline in the number of oocytes begins at 20 weeks' gestation when the female fetus has approximately 6-7 million oogonia (largest lifetime endowment). (medscape.com)
  • Oogonia enter meiosis during embryonic development, becoming oocytes. (wikipedia.org)
  • Despite considerable technological advancements, polyspermy remains a significant challenge in in-vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. (e-jarb.org)
  • [2] It is developed from the primary oocyte by maturation. (wikipedia.org)
  • With IVM treatment , secretion medical care isn't taken to supply massive quantities of mature ovules at the time of gamete extraction, however, immature oocytes pass through the ovaries of ladies and mature within the laboratory for 24-48 hours. (ferty9.co.in)
  • Then, immature oocytes area unit big in ripening media for twenty-four to forty-eight hours. (ferty9.co.in)
  • Embryo transfer is typically done two to five days once the gathering of immature oocytes. (ferty9.co.in)
  • the number} of sex gland follicles and therefore the number of immature oocytes recovered. (ferty9.co.in)
  • Effect of follicle isolation technique on in vitro follicular development, oocyte maturation and embryo development in mice. (yarsi.ac.id)
  • The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
  • Duplicating the complex interaction of growth factors, cellular and hormonal signals required for follicular development and oocyte maturation in vitro is challenging. (biomedcentral.com)
  • [5] Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume. (wikipedia.org)
  • Cycle fecundability decreases as the number of oocytes decreases. (medscape.com)
  • The number of oocytes decreases to approximately 2-3 million at birth and decreases again to 300,000 by the time of puberty. (medscape.com)
  • Oogenesis incorporates a unique sequence of differentiation processes, during which biologically significant events such as meiosis and oocyte growth are orchestrated with communication to surrounding somatic cells. (researchsquare.com)
  • The world's first crossbred bovine embryo transfer calf under tropical conditions was produced by such technique on 23 June 1996 by Dr. Binoy S Vettical of Kerala Livestock Development Board, Mattupatti World usage data is hard to come by but it was reported in a study of 23 countries that almost 42,000 frozen human embryo transfers were performed during 2001 in Europe. (wikipedia.org)
  • It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)
  • 1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)
  • Her clinical and research interests are related to assisted reproductive technologies in horses, with an emphasis on transvaginal oocyte aspiration, in vitro oocyte maturation, fertilization, and embryo culture. (tamu.edu)
  • Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
  • Awakening the oocyte: controlling primordial follicle development. (yarsi.ac.id)
  • From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development. (ivf.net)
  • There has been a dramatic rise in the use of Assisted Reproductive Technologies (ART), including In Vitro Fertilization (IVF) throughout the world over the past three decades. (virascience.com)
  • Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. (biologists.com)
  • After the onset of puberty and menses, the female human ovary recruits at least 30-50 oocytes during each menstrual cycle. (medscape.com)
  • Percoll treatment increased the rate of oocyte penetration and blastocyst formation compared to the control. (e-jarb.org)
  • The large volume of medium significantly reduced the number of dead oocytes and increased the rate of blastocyst formation compared to the small volume. (e-jarb.org)
  • Advancements in embryo culture, blastocyst biopsy techniques, 24-chromosome aneuploidy screening platforms, and improved genomic coverage of new sequencing platforms, such as next-generation sequencing, have made PGT safe and accessible for all patients who undergo in vitro fertilization. (medscape.com)
  • [ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)
  • Results showed that after 8 days in vitro cultured in DMEM containing FSH, the average number of preantral follicles isolated from each whole, partially-cut and completely-cut ovaries were 16.1 + 3.3, 26.8 ± 7.7 and 12.3 + 1.9, respectively. (yarsi.ac.id)
  • Recent Achievements in in vitro culture and preservation of ovarian follicles in mammals. (yarsi.ac.id)
  • Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. (yarsi.ac.id)
  • Beginning on day 4 of culture, we observed significantly higher follicular and oocyte growth rates in follicles co-cultured with AOECs compared with follicles on gel or matrigel. (biomedcentral.com)
  • This model is a novel platform for the in vitro maturation of early follicles and for the future exploration of endothelial-follicular communication. (biomedcentral.com)
  • In vitro development of early follicles necessitates a complex interplay of growth factors and signals required for development. (biomedcentral.com)
  • These drawbacks could be overcome by the ability to mature early follicles in vitro. (biomedcentral.com)
  • The aim of this study was to compare the complication rates of oocyte pick-up (OPU) procedures via transvaginal ultrasonography in patients with different levels of ovarian reserve. (ecerm.org)
  • Oocyte pick-up (OPU) via transvaginal ultrasonography is a commonly used oocyte collection method that is less invasive than other methods [ 4 ]. (ecerm.org)
  • Indeed, efforts have been made to reconstitute in vitro oogenesis, 1-3 though none of them succeeded in the production of functional oocytes from pluripotent stem cells. (researchsquare.com)
  • Here, we established a culture system in which the entire process of oogenesis is reconstituted in a dish, yielding in vitro-generated oocytes that are capable of full-term development to healthy pups. (researchsquare.com)
  • oocytogenesis , ootidogenesis , and finally maturation to form an ovum (oogenesis proper). (wikipedia.org)
  • Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes , a process called oocytogenesis . (wikipedia.org)
  • Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum . (wikipedia.org)
  • Only a few oocytes (eggs) per IVF treatment cycle are able to produce a pregnancy because many eggs have the wrong number of chromosomes. (health.am)
  • Instead, many efforts concentrated on conventional in vitro fertilization (IVF). (ivis.org)
  • Fertility preservation is the technique of conserving or storing one's. (lushfertility.in)
  • Our Medical Director, Dr. Durga G Rao is a pioneer in Fertility Preservation Techniques. (oasisindia.in)
  • On going research focuses on the development of short term in vitro techniques through to whole animal test methods applicable in lab and field settings to evaluate both general reproductive physiology through to the effects seen following exposure to chemicals on reproductive physiology of fish. (uoguelph.ca)
  • Background: Induction of in vitro maturation and development of immature oocyte is one of the methods that has been frequently used recently in artificial reproduction techniques and has drawn attention of many investigators. (virascience.com)
  • Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence. (yarsi.ac.id)
  • The maintaining of oocyte potential viability after longterm storage represent a tool of great opportunity, as it would improve domestic animal breeding by genetic selection programs, contribute to preserve biodiversity through wildlife species conservation, and increase the research in biotechnology. (jove.com)
  • When we have a patient in need of a donor egg/ oocyte, we chose a suitable one from the Donor bank after matching the profile of the lady in need of the donor eggs with the donor. (lushfertility.in)
  • IUI - D (donor) - is an ART (artificial reproductive technique) procedure where in the. (lushfertility.in)
  • Despite major advancements in assisted reproductive techniques, the implantation rates remain relatively not high. (peertechzpublications.com)
  • Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
  • Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)
  • Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
  • Following in vitro maturation, the oocytes were denuded of Cumulus cells mechanically by gently pipetting and examined under stereo microscope, with 60x magnification. (jove.com)
  • First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)
  • Oocytes are surrounded by cells, called cumulus cells, which regulate and assist the process of egg maturation. (health.am)
  • The identification of these genes in cumulus cells can serve as a novel, non-invasive marker to identify abnormal oocytes and thus ultimately improve IVF success rates," said Patrizio, professor in the Department of Obstetrics, Gynecology & Reproductive Sciences at Yale. (health.am)
  • However, it is also possible that such genetic defects result in a smaller complement of oocytes during gonadal development. (ldysinger.com)
  • Importantly, the in vitro-generated oocytes are capable of full-term development. (researchsquare.com)
  • Oocytes were considered mature when they reached metaphase II. (sciencegate.app)
  • The main outcome was the total number of mature oocytes obtained and cryopreserved after IVM. (amsterdamumc.org)
  • In vitro fertilization (IVF), in which fertilization takes place after mature eggs are collected from the ovaries, is a widely used method in the treatment of infertility [ 3 ]. (ecerm.org)
  • For the in vitro experiment, ovarian tissue was extracted and placed in culture plates containing hormone-free medium and media supplemented with the treatment: DHP at 1, 10 and 100 ng mL−1, or P at 10, 100 and 1,000 ng mL−1. (wur.nl)
  • It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. (jove.com)
  • One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
  • In experiment 3, fertilized oocytes were cultured in 25 μL microdroplets (10 gametes/drop) or 500 μL (100 gametes/well) of culture medium in 4-well plates. (e-jarb.org)
  • The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
  • IUI is one of the simple and basic artificial reproductive techniques used in the treatment. (lushfertility.in)
  • Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)
  • Ovarian transplantation is a technique where a small piece of ovarian tissue is cryo-preserved. (oasisindia.in)
  • 11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
  • This technique preserves the right tissue and acts as a solution to the infertility issues arising from these treatments. (oasisindia.in)
  • This discovery may soon allow them to avoid using abnormal - or aneuploid - eggs during infertility treatments, and instead to pick eggs that are healthy enough for a successful in vitro fertilization (IVF) cycle. (health.am)
  • It has been hypothesized that in gonadal dysgenesis various deletions on the X chromosome cause such rapid atresia of oocytes 5 that few girls with this condition reach puberty with functioning ovaries. (ldysinger.com)