Vitro stem cell stem cell. Medical search. Frequent questions

When dissociated, they can be co-cultured with SC-like adipose-derived stem cells (ASC), providing a valuable model to study in vitro nerve regeneration and myelination, mimicking the in vivo environment at the injury site. (jove.com)
In order to further understand the potential use of MSCs to treat dogs with neurological disorders, this study examined the paracrine action of adipose-derived canine MSCs on neuronal and endothelial cell models. (aston.ac.uk)
Hence, this study has demonstrated that adipose-derived canine MSC CM stimulated neuronal and endothelial cells probably through the paracrine activity of MSC-secreted factors. (aston.ac.uk)
We investigated in vitro the paracrine effects of human adipose-derived stem cells (ADSCs) on wound healing mechanisms for human tympanic membrane-derived keratinocytes (hTM) and immortalized human keratinocytes (HaCaT). (oslomet.no)
Cell attachment, proliferation, and osteoblastic differentiation (RT-qPCR and alkaline phosphatase activity) were measured in human adipose-derived stem cells grown on the mantle surface. (port.ac.uk)
A specific population of adipose-derived stem cells (ASCs) is expected to be an alternate source of bone marrow-derived mesenchymal stem cells (BMSCs). (jarde.jp)
however, the adipose-derived stem cells (ASC) have very similar properties and some advantages to be considered a good alternative to BMSC. (oregonstate.edu)

Culture conditions of CD34(+) cells - either with or without mesenchymal stromal cells (MSCs) - had relatively little impact on DNAm, although proliferation is greatly increased by stromal support. (nih.gov)
Bone marrow mesenchymal stem cells (MSCs) have been identified to have the potential to differentiate into multiple types of cells. (nih.gov)
However, the rejection reaction often occurs in the transplantation of MSCs and could induce cell death. (nih.gov)
The primary objective of this study was to verify whether a natural antioxidant, lycopene, could protect MSCs from ischemia-induced apoptosis in vitro. (nih.gov)
Mesenchymal stem cells (MSCs) have been used in cell replacement therapies for connective tissue damage, but also can stimulate wound healing through paracrine activity. (aston.ac.uk)
The culture-expanded MSCs exhibited a MSC phenotype according to plastic adherence, cell morphology, CD profiling and differentiation potential along mesenchymal lineages. (aston.ac.uk)
Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. (nature.com)
To induce in vitro chondrogenesis, MSCs were seeded into MC solution retained within a porous polyurethane (PU) matrix. (nature.com)
This kind of plasticity has been suggested to be a fundamental feature of mesenchymal stem cells (MSCs) as well. (biomedcentral.com)
Mesenchymal stem cells (MSCs) are multi-potent cells that persist in adult life in some tissue types, such as bone-marrow stroma, fat, skeletal muscle, and synovium without loosing their capacity to proliferate and differentiate [ 9 , 10 ]. (biomedcentral.com)
A comparison was done between the properties of NDP-SCs and the properties of mesenchymal stem cells (MSCs) from bone marrow (BM) of the human. (hacettepe.edu.tr)
Mesenchymal stem cells (MSCs) are multipotent progenitor cells with the ability to differentiate into osteoblasts, chondroblasts, myocytes, and adipocytes. (gazi.edu.tr)
Results indicate the feasibility of bone tissue engineering with MSCs and mineralized PLGA scaffolds supporting cell adhesion, viability and osteogenic differentiation properties of cells in hybrid structures under appropriate bioreactor conditions. (gazi.edu.tr)
Controversial results have been reported regarding the anti-tumor properties of extracellular vesicles derived from mesenchymal stem cells (MSCs). (ac.ir)

When cultivated on cell culture dishes coated with 6C3-rich CS/DS, corneal stromal cells (keratocytes) that had been isolated from embryonic chicken corneas formed circular colonies, which took several days to reach confluency. (cardiff.ac.uk)
I claim that the debate over so-called compromise positions in the human embryonic stem cell debate suggests that the purpose of the research for which a research embryo is created is unlikely to be considered as having any significant bearing on the moral permissibility of the practice for those who oppose it. (bmj.com)
NPCs can be differentiated from embryonic stem cells (ESCs) but also from induced pluripotent stem cells (iPSCs) (PMID: 11731782). (sanbio.nl)
We have therefore generated `knock-in' mouse embryonic stem (ES) cells to investigate the effects of expressing a commonly found hot-spot p53 mutant, R246S - the mouse equivalent of human R249S, which is associated with hepatocellular carcinomas. (silverchair.com)
Cell replacement using human embryonic stem cells (hESCs) derivatives may offer new therapeutic opportunities to replace degenerated neurons and repair damaged circuits. (lu.se)
These cancers originate from cells of the sympathetic nervous system (SNS), in turn derived from the transient embryonic neural crest stem cell population. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

Hematopoietic stem and progenitor cells (HPCs) can be maintained in vitro, but the vast majority of their progeny loses stemness during culture. (nih.gov)
Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
List: Human dermal fibroblasts → multilineage blood progenitors (Oct4 and cytokine treatment) Mouse dermal fibroblasts → polygonal hyaline chondrogenic cells (Klf4, c-Myc, Sox9) Mouse dermal fibroblasts → cardiomyocytes (Oct4, Sox2, Klf4, JI1 and Bmp4) Fibroblasts → neural stem/progenitor cells (Oct4, Sox2, c-Myc, Klf4) Transdifferentiation Induced stem cells Pournasr, B. (wikipedia.org)
Figure 3) or PSA-NCAM are markers frequently used for intermediate progenitor cells and early immature neurons (PMID: 29625071). (sanbio.nl)
Early fate decisions in hematopoietic stem and progenitor cells. (lu.se)

In vitro osteogenic differentiation of r. (gazi.edu.tr)
13. Wang X, Omar O, Vazirisani F, Thomsen P, Ekström K. Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation. (ac.ir)
More osteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture. (utwente.nl)

article{ff9b980d-2e89-453c-b22b-428d2e903c6f, abstract = {{Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)

They have potential for bone tissue engineering by the utilization of in vitro expanded cells with osteogenic capacity and their delivery to the appropriate sites via biomaterial scaffolds. (gazi.edu.tr)
This series covers biophysical regulation of stem cell fate and function in the context of tissue development. (biomedcentral.com)
Advances in the fields of stem cell biology, biomaterials, and tissue engineering over the last decades have brought the possibility of constructing tissue substitutes with a broad range of applications in reg. (biomedcentral.com)
In this regard, tissue engineers have sought to leverage native mechanotransduction pathways to enhance in vitro stem cell. (biomedcentral.com)
Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. (biomedcentral.com)
thus, they are promising for facilitating self-organizing bone/cartilage tissue formation in vitro from induced pluripotent stem cells (iPSCs). (elsevierpure.com)
A subset of NSCs is present in the developed nervous system and acts as a reservoir of cells for cell replacement and nervous tissue regeneration (PMID: 20110496). (sanbio.nl)
In vitro and in vivo results implied that Cbfa1-overexpressing ASCs may be an alternative cell source for BMSCs for bone tissue engineering. (jarde.jp)
5. Ahn J-O, Coh Y-R, Lee H-W, Shin I-S, Kang S-K, Youn H-Y. Human adipose tissue-derived mesenchymal stem cells inhibit melanoma growth in vitro and in vivo. (ac.ir)
Development of good in vitro human tissue models would help to bridge the gap in our current knowledge of lung responses and provide a better understanding of lung development, physiology and pathology. (biomedcentral.com)
A benefit of current in vitro lung models is that hypotheses generated from review of data from human or animal disease studies can be tested directly in engineered human tissue models. (biomedcentral.com)
Subsequent work focused on the construction of alveolar-like tissue structures using primary type II cells grown in three-dimensional (3D) collagen gels that supported production of surfactant proteins [ 5 ]. (biomedcentral.com)
The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source for bone tissue engineering. (utwente.nl)
These results define BFP as a new, rich, and accessible source of stem cells for tissue engineering purposes. (utwente.nl)
The purpose of this study was to fabricate and evaluate the osteogenic potential diferentiation of human amnion mesenchymal stem cell with carbonate apatiteâ€"chitosan scaffolds (CA-ChSs) for tissue engineering. (ejournals.ca)
hAMSc harvested from human amniotic membrane seeding in CA-ChSs had the capability for in vitro osteogenesis makes them be the one of the potential options for bone tissue engineering. (ejournals.ca)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
Materials and method: The pulp tissue, obtained from wisdom teeth, was placed in a 6-well plate containing proper culture medium, and stored at 37 °C and 5% CO2 for cell proliferation and plastic adherence. (bvsalud.org)
The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

In this webinar, Dr. Cedric Bardy talks about why and how he invented BrainPhys™, a new neuronal basal medium that better supports neuronal function in vitro, described in C Bardy et al. (stemcell.com)
Dr. Bardy describes how BrainPhys™ increases the physiological relevance of primary and hPSC-derived neuronal cultures in vitro. (stemcell.com)
Neural stem cells (NSCs) are undifferentiated neural cells with the capacity for long-term self-renewal and for differentiation into all types of neuronal and glial cells. (sanbio.nl)
Neural progenitors are precursor cells able to further differentiate into various neuronal and glial cells. (sanbio.nl)
This can be achieved in neuronal monocultures using phase images obtained with Essen Bioscience's IncuCyte ZOOM™ live-cell imaging platform followed by analysis with IncuCyte NeuroTrack™ software. (genengnews.com)
In this tutorial, we describe a method for visualizing and quantitating neurite outgrowth in co-culture with astrocytes, utilizing a novel fluorescent label for neuronal processes and the IncuCyte ZOOM live-cell imaging system. (genengnews.com)
The reagent uses a synapsin promoter to drive neuronal expression of the red fluorescent protein mKate2 and minimize crossover to non-neuronal cells. (genengnews.com)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

Is the Subject Area "Hematopoietic stem cell transplantation" applicable to this article? (plos.org)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
Human mesenchymal stem cells (hMSCs) is viable for xenogenic transplantation. (ejournals.ca)
The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
My projects investigated whether patient-derived cells are more prone to develop disease phenotypes and what can be done to protect these cells after transplantation," explained Fredrik. (lu.se)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
These stem cells are most often used for transplantation. (msdmanuals.com)
Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
Hematopoietic stem cell transplantation is currently an inpatient procedure. (medscape.com)
A study by MacMillan et al reported that alternative donor hematopoietic cell transplantation had a high success rate in patients with Fanconi anemia who did not have a history of opportunistic infections or transfusions and who underwent conditioning with single fraction total body irradiation 300 cGy, cyclophosphamide, fludarabine, and antithymocyte globulin. (medscape.com)
Although the only therapy that can cure the pancytopenia is stem cell transplantation, androgens, to which approximately 50-75% of patients respond, are used for those in whom transplantation is not an option. (medscape.com)

A flow cytometric analysis of these keratocytes revealed changes in their expression levels of the indicative stem cell markers, Connexin 43 (Cx43), Paired Box 6 (PAX6), B-lymphoma Moloney murine leukemia virus insertion region-1 (Bmi-1), and C-X-C Chemokine Receptor 4 (CXCR4) suggestive of a less-differentiated phenotype compared with expression levels in cells not exposed to CS/DS. (cardiff.ac.uk)
These findings support the view that CS/DS promotes the retention of a stem cell phenotype in corneal cells, much as it has been proposed to do in other connective tissues. (cardiff.ac.uk)
EE selectively increased cell differentiation into MSN phenotype and promoted host-to-graft connectivity. (lu.se)

We used the pancreatic adenocarcinoma cell line PANC-1 as a model to apply different chemotherapeutic protocols (monotherapy and combined therapy) using 5-Fluorouracil, Oxaliplatin, Irinotecan, Gemcitabine and Abraxane. (isciii.es)
Currently, the only FDA-approved cell-based therapy for cartilage defects involves autologous chondrocyte implantation: chondrocytes harvested from low-contact areas are expanded in vitro and then re-injected directly into the damaged site 1 . (nature.com)
An article collection in Stem Cell Research & Therapy . (biomedcentral.com)
Eliminating ROS elimination or inhibiting AKT blocked the effects of the laser therapy in vivo and in vitro. (google.com)
16. Wang J, Zheng Y, Zhao M. Exosome-Based Cancer Therapy: Implication for Targeting Cancer Stem Cells. (ac.ir)
Cell Replacement Therapy for Parkinson's Disease - Evaluating the potential of autologous grafting. (lu.se)
For the last four years, Fredrik has explored the use of patient-specific cells in cell replacement therapy for Parkinson's disease to identify strategies to safeguard these transplanted cells from disease-related degeneration. (lu.se)
My Ph.D. studies revolved around cell replacement therapy for Parkinson's disease. (lu.se)
My thesis cover illustrates the workflow of generating patient-specific cells for cell replacement therapy for Parkinson's Disease. (lu.se)
Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)
The aim of this study was to analyze the influence of low-level laser therapy (=660 nm), with three different irradiation times, on the behavior of OD-21 cell line. (bvsalud.org)
One such idea is cell replacement therapy (CRT). (lu.se)

We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
Here, in the presence of KL+FL+megakaryocyte growth and development factor (MGDF), which recruits virtually all Lin(-)Sca-1(+)kit(+) bone marrow cells into proliferation and promotes their self-renewal under serum-free conditions, IL-3 and IL-11 revealed an indistinguishable ability to further enhance proliferation. (lu.se)
For example, temozolomide (TMZ), of use for glioblastoma (GBM) treatment, appears as capable of inducing autophagy partially inhibiting cancer cell proliferation. (nature.com)
The objective of the present study was to investigate whether OSM affects the proliferation and differentiation of rat stem Leydig cells . (bvsalud.org)
We used a Leydig cell regeneration model in rat testis and a unique seminiferous tubule culture system after ethane dimethane sulfonate (EDS) treatment to assess the ability of OSM in the regulation of proliferation and differentiation of rat stem Leydig cells . (bvsalud.org)
Analysis indicated a larger and more coordinated transcriptomic adaptation during adipogenesis compared to osteogenesis, with a larger induction of metabolism, particularly lipid synthesis (mostly triglycerides), and a larger use of amino acids for synthesis of feed-forward adipogenic compounds, larger cell signaling, lower cell-to-cell interactions, particularly for the cytoskeleton organization and cell junctions, and lower cell proliferation. (oregonstate.edu)
Conclusion: Our results suggest that the explant method - or cell proliferation method - is suitable for the isolation and culture of stem cells from dental pulp of permanent teeth. (bvsalud.org)
Irradiation time of group I influenced positively the proliferation and viability of OD-21 cells in late cell culture period. (bvsalud.org)

Transcription Adaptation during In Vitro Adipogenesis and Osteogenesis of Porcine Mesenchymal Stem Cells: Dynamics of Pathways, Biological Processes, Up-Stream Regulators, and Gene Networks. (oregonstate.edu)
In the present study we have used transcriptome analysis to unravel pathways and biological functions driving in vitro adipogenesis and osteogenesis in BMSC and ASC. (oregonstate.edu)

Immunofluorescent staining of PAX6 ( 12323-1-AP , 1:250 dilution) with 4% PFA fixed control human-induced pluripotent stem cell (hiPSC)-derived NPCs. (sanbio.nl)
To complement the in vivo system of chick embryos, we employ patient omics data, single cell RNA sequencing, human and mouse embryos, human cancer cell cultures, human pluripotent stem cells, chick embryo neural crest-derived in vitro crestosphere cultures, and zebrafish and mouse in vivo models. (lu.se)
Skin cells are taken from a patient, reprogrammed to induced pluripotent stem cells (iPSCs), differentiated into dopaminergic progenitors, and put back in the patient. (lu.se)
No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)

These studies have demonstrated that mechanical forces are able to shape the mesenchymal stem cell (MSC) fate when appropriately applied in in vitro models of cartilage regeneration. (nature.com)
Intratesticular injection of OSM (10 and 100 ng/ testis ) from post-EDS day 14 to 28 blocked the regeneration of Leydig cells by reducing serum testosterone levels without affecting serum luteinizing hormone and follicle-stimulating hormone levels. (bvsalud.org)
The importance of mesenchymal stem cells (MSC) for bone regeneration is growing. (oregonstate.edu)
Fibroblast stem cells or stemlike cells (FSCs) are proposed to play a pivotal role in extracellular matrix (ECM) regeneration by serving as a key source of ECM-producing fibroblasts. (cdc.gov)
Over the last years, dentistry has been exploring the potential application of stem cells from different origins in the regeneration of oral tissues that were lost or damaged by disease or trauma 1 . (bvsalud.org)

Keratinocytes cultured in CMADSC showed a significant increase in cell number compared to serum-free cultures and further significant increases in hypoxic CMADSC. (oslomet.no)
Amniotic membrane obtained using cesarean section under aseptic condition did not exhibit any growth of cell cultures which were not contaminated. (ejournals.ca)
Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)

Oncostatin M inhibits differentiation of rat stem Leydig cells in vivo and in vitro. (bvsalud.org)

Accordingly, investigations on cellular therapies have therefore moved to progenitor cell populations such as bone marrow derived mesenchymal stem cells (BMSCs), which have the ability to differentiate into cartilage cells 4 . (nature.com)
Stem cells differentiate into bone-forming osteoblasts, and mechanical stimulation is involved in this p. (biomedcentral.com)

The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)
After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)
Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
In vitro cultivated stem cell populations are in general heterogeneous with respect to their expression of differentiation markers. (biomedcentral.com)
NPC markers are also useful in establishing and monitoring in vitro NPC culture differentiation. (sanbio.nl)
Alternatively, NPCs can be isolated from fresh nervous tissues using cell sorting with NPC markers (PMID: 11121071). (sanbio.nl)
The focus-forming cells exhibited stem properties as indicated by stem cell markers expression, sphere formation, and ALDH activity assays. (cdc.gov)
Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)

J Cell Mol Med;23(1): 426-438, 2019 01. (bvsalud.org)

In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
Propagation of human spermatogonial stem cells in vitro. (wakehealth.edu)
The present study was conducted to evaluate whether secretome derived from Human Wharton's jelly mesenchymal stem cells (hWJMSCs) may stimulate or inhibit breast cancer growth in vitro and in vivo. (ac.ir)
Mesenchymal stem cells derived from human adipose tissues favor tumor cell growth in vivo. (ac.ir)
Vaccination with human amniotic epithelial cells confer effective protection in a murine model of Colon adenocarcinoma. (ac.ir)
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma. (ac.ir)
Human bone marrow mesenchymal stem cells display anti-cancer activity in SCID mice bearing disseminated non-Hodgkin's lymphoma xenografts. (ac.ir)
Naive human umbilical cord matrix derived stem cells significantly attenuate growth of human breast cancer cells in vitro and in vivo. (ac.ir)
In vitro models of simple one-cell or two-cell culture systems as well as complex multicellular lung analogs that reproduce defined components of specific human lung responses have already been realized. (biomedcentral.com)
Because of these problems, most researchers have utilized in vitro cell culture models to answer basic questions related to human cellular responses in the lung. (biomedcentral.com)
Formation of human composite respiratory mucosa using normal human bronchial cells cocultured with bone marrow mesenchymal stem cells under air-interface conditions allowed for the formation of mucus-producing cells in vitro [ 12 ]. (biomedcentral.com)
The knock-in cells contain higher levels of p53 that localizes to the nucleus even in the absence of genotoxic stress and yet remains non-functional, reminiscent of mutant p53 found in human tumours. (silverchair.com)
Restored in vivo-like membrane lipidomics positively influence in vitro features of cultured mesenchymal stromal/stem cells derived from human placenta. (remembrane.com)
Conclusions: The data support the long-term therapeutic potential of ESC-derived human striatal progenitor grafts for the replacement of degenerated striatal neurons in HD and suggest that EE can effectively accelerate the maturation and promote the integration of human striatal cells. (lu.se)
Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

What are neural stem cells (NSCs), neural progenitors, and neural precursors (NPCs)? (sanbio.nl)

We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)

Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)

MTT assays was performed to determine anti-tumor effects of hWJMSCs-secretome on both MCF-7 and 4T1 tumor cells in vitro. (ac.ir)

We developed a mechanism-based in vitro model for fibrogenicity testing of nanomaterial s based on their ability to induce FSCs. (cdc.gov)
One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)

Neural stem cells (NSCs) obtained from the fetal mouse hippocampus were cultured in the collagen-chitosan composite scaffolds and the biocompatibility was studied. (ox.ac.uk)

NPCs can additionally be obtained by direct reprogramming of somatic cells from different cell lineage (PMID: 22445518). (sanbio.nl)
NPCs are typically grown in vitro in the form of neurospheres or as adherent monolayers (PMID: 26798357). (sanbio.nl)

One of the earliest models used to study lung development, an in vitro culture of pieces of intact fetal lung, indicated that there were critical differences in the development of submersion-cultured fetal lung cell- scaffold constructs versus air-interface-cultured fetal lung constructs [ 4 ]. (biomedcentral.com)

Our results demonstrate that HPCs acquire DNA-hypermethylation at specific sites in the genome which is relevant for the rapid loss of stemness during in vitro manipulation. (nih.gov)

Since cancer stem cells have been hypothesized to play a role in refractory/relapsing cancers, in the present work we investigated if autophagy could represent a constitutive cytoprotection mechanism for glioblastoma stem-like cells (GSCs) and if the modulation of autophagic process could affect GBM growth and survival. (nature.com)
Death of GSCs was apparently due to the iron dependent form of programmed cell death characterized by the accumulation of lipid peroxides called ferroptosis. (nature.com)
The failure of current therapies may be due to the presence, within the tumor, of cells characterized by enhanced self-renewal capacity, multilineage differentiation potential and elevated invasive behavior, called glioma stem cells (GSCs). (unimib.it)

Cancer stem cells are responsible for tumor chemoresistance and recurrence in adjuvant and metastatic settings. (isciii.es)
Recurrence and chemoresistance in pancreatic cancer are largely related to cancer stem cells. (isciii.es)

How did you end up doing a Ph.D. at Lund Stem Cell Center? (lu.se)
During a biology course, we had a guest speaker from Lund Stem Cell Center who talked about stem cells and their potential to become any type of cell in the body. (lu.se)
I found it incredibly interesting so when it came to doing a master's project, I reached out to Lund Stem Cell Center. (lu.se)
What have you found the most enjoyable during your Ph.D. studies at Lund Stem Cell Center? (lu.se)

The generation and maintenance of replenishing tissues relies on an appropriately regulated balance between self-renewal and differentiation within a relatively small population of adult stem cells. (biomedcentral.com)
Dental pulp stem cells were primarily derived from the pulp tissues of exfoliated deciduous teeth, primary incisors and permanent third molar teeth. (hacettepe.edu.tr)
Stem cells reside within most tissues throughout the lifetimes of mammalian organisms. (biomedcentral.com)
Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)

In the Dentistry field, stem cells derived situated in red and near-infrared region of electromagnetic from dental pulp have been isolated and investigated as spectrum. (bvsalud.org)

Tras analizar mediante RT-qPCR diferentes marcadores de células madre tumorales (SOX2, OCT4, CD133, CD44 y CD24) en células de cáncer de páncreas tratadas con diferentes protocolos quimioterapéuticos, el Oxaliplatino y la Gemcitabina en monoterapia fueron los quimioterápicos que seleccionaron en mayor medida las células madre cancerosas mientras que el protocolo FOLFIRI las disminuyó. (isciii.es)

Even though in vitro gametogenesis could serve as a powerful new technology to overcome infertility, I argue that opponents of the practice of creating embryos solely for research purposes would still view the creation of research embryos that the development of in vitro gametogenesis would require, as being incompatible with affording the embryo proper moral respect. (bmj.com)
I conclude by suggesting that Sparrow's analysis of the potential benefits of in vitro gametogenesis provides us with further reasons to scrutinise the unconvincing arguments that are often cited in favour of prohibiting the practice of creating embryos solely for research purposes. (bmj.com)
Stem cells can be obtained from the embryos that are not used. (msdmanuals.com)

This paper gives an Islamic perspective on some of these advances, including abortion, in vitro fertilization, genetic engineering, cloning and stem cell research. (who.int)

To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
At Axion BioSystems, our goal is to help you capture the complex biology of your cells. (axionbiosystems.com)
Nature Cell Biology. (wikipedia.org)

Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)

Here, we study MSC plasticity on the level of individual cells applying a multi-scale computer model that is based on the concept of noise-driven stem cell differentiation. (biomedcentral.com)
Our predictions on MSC plasticity and its dependence on the environment motivate a number of in vitro experiments for validation. (biomedcentral.com)
Currently, there is an ongoing debate on fundamental dynamics underlying this kind of cell plasticity. (biomedcentral.com)
According to this concept, MSC plasticity bases on permanent fluctuations of the differentiation state of each individual cell, which enables more differentiated cells to re-gain stem cell properties and subsequently to switch lineage (details see below). (biomedcentral.com)

Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
The only stimulant that enhanced PAC paracrine angiogenic activity was atorvastatin, which also had ability to stabilize endothelial tubes in vitro . (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)

Besides the cell source choice, also mechanical stimuli are crucial in the development and maintenance of articular cartilage. (nature.com)
Chondroitin sulphate (CS) proteoglycans with variable sulphation-motifs along their glycosaminoglycan (GAG) chains are closely associated with the stem cell niche of articular cartilage, where they are believed to influence the characteristics of the resident stem cells. (cardiff.ac.uk)

Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
Together with gene regulation associated with hypoxia in ADSCs, this study has provided several strong leads for a stem cell-derived approach to TM wound healing. (oslomet.no)
This study also aimed to assess the expression of ALDH1 (aldehyde dehydrogenase 1) enzyme activity on the isolated cells. (bvsalud.org)
The study, which involved 130 patients, also found that adding fludarabine to conditioning caused three-fold enhancement of hematopoietic cell engraftment. (medscape.com)

Axion BioSystems' live-cell assay technology can help you record it noninvasively, in real time, and with software tools to make analysis quick and painless. (axionbiosystems.com)
To evaluate the pharmacological efficacy of selected drugs on six GSC lines, we set up a multiple drug responsivity assay based on the combined evaluation of cytomorphological and functional parameters, including the analysis of polymorphic nuclei, mitotic index and cell viability. (unimib.it)
Here we describe a medium throughput in vitro assay providing kinetic measurements of neurite dynamics in a co-culture model of neurons and astrocytes. (genengnews.com)
Figure 2 shows data from a 96-well assay measuring neurite length over twelve days using NeuroLight Red labeled rat forebrain neurons with rat cortical astrocytes (NeuroPrime™ Cell Kit). (genengnews.com)

Anatomy57

Organisms16

Diseases12

Chemicals and Drugs67

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Phenomena and Processes51

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science2

Named Groups1

Health Care1

Adipose-derived7

MSCs14

Embryonic9

Progenitor cells9

OSTEOGENIC DIFFERENTIATION3

Abstract1

Tissue22

Neuronal10

Transplantation15

Phenotype3

Therapy12

Proliferation8

Osteogenesis2

Pluripotent7

Regeneration5

Cultures3

Differentiation of rat1

Differentiate2

Markers9

20191

Human21

Progenitors1

Donor4

Endothelial cells3

Tumor cells1

Induce2

Neural stem1

PMID2

Scaffold1

Stemness1

GSCs3

Cancer stem2

Lund-stem-cell-center4

Tissues4

Dental pulp1

OCT41

Embryos3

Genetic1

Biology3

Fetal1

Plasticity4

Angiogenic4

Cartilage2

Study4

Assay4