• and promoted the proliferation and invasiveness of breast cancer cells both in vivo and in vitro. (cancerindex.org)
  • Our facility also offers several proteomic relative quantification strategies, including label-free analysis and stable isotope labeling, both in vivo and in vitro, using a variety of labeling tags. (edu.pl)
  • In addition to the standard mouse lethality bioassay, many in vivo and in vitro analytical methods have been recently developed for the determination of a BoNT's identity and concentration for the purposes of diagnosis and confirmation of botulism 15 - 17 . (cdc.gov)
  • To carry out these legislative mandates, ATSDR's Division of Toxicology (DT) has developed and coordinated a mixtures program that includes trend analysis to identify the mixtures most often found in environmental media, in vivo and in vitro toxicological testing of mixtures, quantitative modeling of joint action, and methodological development for assessment of joint toxicity. (cdc.gov)
  • Moreover, a significant loss of Mrp2 protein in the membrane fraction of rat cryopreserved hepatocytes was observed. (aspetjournals.org)
  • N-Glycosylation plays an important role in the structure and function of membrane and secreted proteins. (cdc.gov)
  • By developing new stable isotope labeling techniques, we will push forward the NMR analysis of HMW proteins and membrane proteins, including important drug targets. (u-tokyo.ac.jp)
  • Through applying cutting edge techniques like advanced light microscopy, cell-free protein synthesis and microfluidics, we seek to systematic investigation of membrane proteins as well as bottom-up assembly of minimal cellular systems. (ezreregister.com)
  • A refined model of water and CO2 membrane diffusion: Effects and contribution of sterols and proteins. (ezreregister.com)
  • High Level Cell-Free Production of Membrane Proteins into Nanodiscs. (ezreregister.com)
  • Co-translational insertion of cell-free expressed membrane proteins into lipid bilayers. (ezreregister.com)
  • Synaptobrevin on the synaptic vesicle must interact with syntaxin and SNAP (synaptosomal-associated protein)-25 on the neuronal membrane for fusion to occur, which allows the nerve impulse to be delivered across the. (cdc.gov)
  • Depending on the length of their fatty acid chains and their degree of saturation, SL species have a strong impact on biophysical membrane parameters such as fluidity or rigidity and curvature, and on interaction with membrane proteins and/or cytoskeletal components, and membrane compartmentalization. (encyclopedia.pub)
  • Our main services include quantitative and qualitative proteomic approaches, PTM analysis, and the targeted quantification of protein panels. (edu.pl)
  • Recently, the data-independent acquisition (DIA) analysis has been added to the spectrum of our offered methods, which enables protein quantification in complex samples in a more accurate manner than the aforementioned data-dependent techniques. (edu.pl)
  • However, because of the unknown binding affinity of antibody to MRP2/Mrp2 protein across species and lack of purified MRP2/Mrp2 proteins for calibration, the immunoblotting assay was excluded from the absolute quantification of MRP2/Mrp2 protein for multiple species. (aspetjournals.org)
  • Our method allows for the identification of the proteins, identification of redox-sensitive cysteines within proteins, and quantification of the redox status of individual cysteine-containing peptides. (hindawi.com)
  • Thus, reversible cysteine modifications can influence protein activity and the relative quantification of the status of the thiol can potentially provide valuable insights into protein activity where the protein exists in a range of redox states. (hindawi.com)
  • In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)
  • Here, we report the use of a targeted liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method for concurrent quantification of multiple cytochrome P450 (P450), UDP-glucuronosyltransferase (UGT), and transporter proteins in cultured primary human hepatocytes. (aspetjournals.org)
  • Direct protein quantification was applied successfully to study in vitro induction in sandwich cultured primary hepatocytes in a 24-well format using the prototypical inducers rifampicin, omeprazole, and phenobarbital. (aspetjournals.org)
  • We conclude that targeted absolute LC-MS/MS quantification of drug-metabolizing enzymes and transporters can broaden the scope and significantly increase the impact of in vitro drug metabolism studies, such as induction, as an important supplement or future alternative to mRNA and activity data. (aspetjournals.org)
  • Usually synthetic or recombinant proteins are used as isotopic standards and spiked into samples to serve as internal standards for quantification. (genengnews.com)
  • Quantitative proteomic methods fall into two general categories: label-based or label-free quantification. (openaccesspub.org)
  • Quantification of ~250-800 different proteins among three different types of neurons revealed reproducible proteomic differences between the neuron types. (njacs.org)
  • A suite of in vitro assays and in silico models were evaluated to identify which best detected the endocrine-disrupting (ED) potential of 10 test chemicals according to their estrogenic, androgenic and steroidogenic (EAS) potential compared to the outcomes from ToxCast. (bvsalud.org)
  • In vitro methods included receptor-binding, CALUX transactivation, H295R steroidogenesis, aromatase activity inhibition and the Yeast oestrogen (YES) and Yeast androgen screen (YAS) assays. (bvsalud.org)
  • In silico final calls were mostly in agreement with the in vitro assays, and predicted ER and AR effects well. (bvsalud.org)
  • These can be combined with the YES/YAS assays in an initial screen of chemicals in the early tiers of an NGRA to inform on the MoA and the design of mechanistic in vitro assays used later in the assessment. (bvsalud.org)
  • Cell based and in vitro assays suggested that ADNP does not bind to H3K9me3 directly but is targeted to this modification by HP1. (uni-goettingen.de)
  • Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)
  • In contrast to steady-state protein abundance, which may be quantified directly in mass spectrometry (MS) by spectral intensity 16 , 17 or sampling frequency 18 , 19 , protein turnover rates cannot be predicted from steady-state data 20 , requiring instead methods that can distinguish old and new protein molecules in mass spectra 11 , 21 . (nature.com)
  • Mass spectrometric approaches to protein structure characterization are offered, including hydrogen-deuterium exchange, X-linking, native mass spectrometry, and ion mobility. (edu.pl)
  • We used two independent discovery-based approaches-microarray gene expression and stable isotope labeling by amino acids in cell culture (SILAC) quantitative proteomic mass spectrometry analyses-to identify cellular phenotypes in SZ hiPSC NPCs from four SZ patients. (ca.gov)
  • Improvements in mass spectrometry has led to the development of a number of techniques to quantify the relative protein abundance within a given sample. (hindawi.com)
  • We investigated the entire set of proteins modulated by BAG3 silencing in the human anaplastic thyroid 8505C cancer cells by using the Stable-Isotope Labeling by Amino acids in Cell culture strategy combined with mass spectrometry analysis. (oncotarget.com)
  • Based on recent developments in quantitative mass spectrometry, biological reference materials have been generated that can be used as internal (quality) controls for proteomics utilizing the characteristics of isotope-dilution mass spectrometry (ID-MS). (genengnews.com)
  • Using the cutting-edge mass spectrometry-based proteomic techniques, she has been focused on elucidating mechanisms of tissue-specific breast cancer metastasis and identifying protein targets to eradicate the growth of distal breast cancer metastasis. (openaccesspub.org)
  • Mass spectrometry (MS)-based proteomics has become a powerful technology to quantify the entire complement of proteins in cells or tissues. (biomed.news)
  • In large-scale quantitative mass spectrometry (MS)-based phosphoproteomics, isobaric labeling with tandem mass tags (TMTs) coupled with offline high-pH reversed-phase peptide chromatographic fractionation maximizes depth of coverage. (biomed.news)
  • For a better understanding of factors involved with heterochromatin I used stable isotope labeling by aminoacids in cell culture (SILAC) in a H3K9me3 pull-down experiment to identify new interaction partners. (uni-goettingen.de)
  • Their tiny size, rapid growth by clonal propagation, and facile uptake of labeled compounds from the media were attractive features that made them a well-known model for plant biology from 1950 to 1990. (osti.gov)
  • In many cases, drug discovery starts from screening compounds that bind to the target in vitro. (u-tokyo.ac.jp)
  • To confirm differential proteins that are revealed by the above techniques and develop protein diagnostic panels, targeted proteomics methods based on multiple reaction monitoring/parallel reaction monitoring (MRM/PRM) data acquisition protocols are applied. (edu.pl)
  • Redox proteomics has taken advantage of the thiol specificity of ICAT reagents not only to identify targets of ROS but also to quantify oxidative thiol modifications in individual proteins. (hindawi.com)
  • We also examined the dataset of proteins obtained by the quantitative proteomics analysis using two tools, Downstream Effect Analysis and Upstream Regulator Analysis of the Ingenuity Pathways Analysis software. (oncotarget.com)
  • Proteomics is rapidly progressing with many important applications in protein biomarker discovery and validation. (genengnews.com)
  • Given the high number of proteins used in the clinic, proteomics is poised to have a massive impact on in vitro diagnostics (IVD), drug development, and rapid assay development. (genengnews.com)
  • Over the last few years, the increasing number of proteomics studies has led to thousands of nonvalidated protein biomarker candidates for which little supportive data is available. (genengnews.com)
  • Therefore, the development of methods for accurate protein quantitation is one of the most challenging areas of proteomics. (openaccesspub.org)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • Each serotype of BoNT hydrolyzes their protein substrates at different peptide bonds. (cdc.gov)
  • Currently standards must be generated one by one by incorporating isotope-labeled amino acids into the peptide sequence by chemical synthesis or by addition to culture media. (genengnews.com)
  • The devices and kits include one or more associators specific for molecular targets, such as microRNAs and proteins and peptide fragments thereof. (justia.com)
  • To investigate to what extent limited sample amounts affect sensitivity and dynamic range of the analysis due to sample losses, we benchmarked TMT-based fractionation strategies against single-shot label-free approach with spectral library-free data independent acquisition (LFQ-DIA), for different peptide input per sample. (biomed.news)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • In a typical REU project, students analyze sequence data to identify mutations that could influence the evolution of protein function, then construct the mutations, express, purify and assay the enzymes to determine the role of the mutated position. (tamu.edu)
  • In general, two different methods of chemical labeling are used-isotopic mass labeling and isobaric mass labeling. (genengnews.com)
  • Isobaric mass labeling leads to isobaric analytes that can be differentiated by isotopic mass reporters released from the analyte during tandem mass spec. (genengnews.com)
  • Labels such as isobaric chemical tags with different masses can be introduced through covalent modification to peptides. (openaccesspub.org)
  • The results could potentially fill the translational gaps of in vitro/in vivo or preclinical species to human extrapolation of hepatobiliary elimination mediated by MRP2/Mrp2. (aspetjournals.org)
  • The assessment of potential drug-drug interactions by in vitro methods has become an indispensable part of the preclinical development process of new drug candidates. (aspetjournals.org)
  • Uncharacterized sub-cellular localization of some non-CYP enzymes is another knowledge gap with respect to the development of quantitatively viable in vitro and in silico models. (njacs.org)
  • Several fertility restoration approaches, whose aim is to mature cryopreserved tissues in vivo or in vitro in order to produce spermatozoa, are being developed. (elifesciences.org)
  • Protein dynamics data are therefore sought to better describe homeostatic processes and enhance the utility of phenotyping-by-omics approaches. (nature.com)
  • These MS-based quantitative approaches using label-free, tags, or stable isotope labeling have their own strengths and limitations. (openaccesspub.org)
  • Using these quantitative approaches, one can investigate the dynamics of proteome through differential protein expression in normal biological processes and diseases. (openaccesspub.org)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • The light chain is a zinc-metalloprotease which is responsible for the cleavage of three SNARE complex proteins including SNAP-25 (Synaptosome-associated protein of 25 kDa), synaptobrevin-2 (also termed VAMP-2) and syntaxin. (cdc.gov)
  • The dataset contains over 1.92 million data points in protein isotope labeling kinetics, culminating as the in vivo turnover rates of 3,228 cardiac proteins and the expression levels of 8,064 proteins. (nature.com)
  • Students will learn about protein expression and purification, and measurements of binding affinity and kinetics. (tamu.edu)
  • In vitro studies, which have toxicity assessment has proceeded with limited consideration of the become an essential component of risk assessment-directed unique kinetics of these materials in solution. (cdc.gov)
  • We demand to characterize the hazard potential of the considerable develop these ideas by introducing the concept of cellular dose number of nanomaterials that have been or will be produced is in vitro as an important dose metric and by integrating aspects of material science, solution physics, and kinetics to present the 1 factors and processes affecting the cellular dose for particles. (cdc.gov)
  • In NMR analyses of proteins, the atoms of interest can be selectively observed by labeling appropriate atoms with stable isotopes such as 2 H, 13 C, and 15 N. Especially, high-level deuteration of protons surrounding observed atoms enables sensitive detection of NMR signals in combination with the TROSY methods. (u-tokyo.ac.jp)
  • In addition, the number of samples analyzed in single studies is not increasing to match appropriate statistical methods but rather is stable or even decreasing. (genengnews.com)
  • N-glycosylation profoundly affects the biological stability and function of therapeutic proteins, which explains the recent interest in glycoengineering technologies as methods to develop biobetter therapeutics. (umd.edu)
  • accepted October 27, 2006 one of the most significant challenges faced by the regulatory, The rapid growth in the use of in vitro methods for nanoparticle research, and producer communities. (cdc.gov)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
  • Histological, RT-qPCR, western blot analyses and steroid hormone measurements were performed on in vitro cultured mouse prepubertal testicular tissues and age-matched in vivo controls. (elifesciences.org)
  • Our analyses confirm the association of the proteome profile observed in BAG3-silenced cells with an increase in cell survival and a decrease in cell proliferation and invasion, and highlight the possible involvement of four tumor suppressor miRNAs and TP53/63 proteins in BAG3 activity. (oncotarget.com)
  • We put efforts on developing methodologies that can elucidate the functional mechanisms of high-molecular-weight proteins, establish structure-function relationship analyses that do not rely on mutants, and introduce intracellular structure-guided drug development. (u-tokyo.ac.jp)
  • This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. (blogspot.com)
  • Protein stability is a major regulatory principle of protein function and cellular homeostasis. (nature.com)
  • Proteins with quantified dynamics belong to over 10 major cellular compartments and over 200 known pathways. (nature.com)
  • RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
  • In other scenarios, quantitative measurements derived from the MS technology can be used to associate cooperative protein expression changes with cellular responses such as cell signaling. (openaccesspub.org)
  • Botulinum neurotoxins (BoNTs) are proteases that cleave specific cellular proteins essential for neurotransmitter release. (cdc.gov)
  • BoNTs are zinc metalloproteases that cleave and inactivate specific cellular proteins essential for the release of the neurotransmitter acetylcholine ( Figure 1 ). (cdc.gov)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • Proteomic services include protein mass determination, protein identification from gel or solution, qualitative and semi-quantitative characteristics of the protein interactome, and the characterization and mapping of a wide spectrum of posttranslational modifications. (edu.pl)
  • Lipid synthesis requires bulk carbon skeleton acyl-CoAs, the transport of which depends on the acyl-CoA binding protein (ACBP). (biomedcentral.com)
  • Before entering lipid synthesis pathways, different kinds of acyl-CoA transporters which acts as the major carrier of acyl-CoAs, such as fatty acid binding protein (FABP), sterol carrier protein 2 (SCP2) and acyl-CoA-binding protein (ACBP), would activate and convert fatty acids to fatty acyl-CoA esters via a reaction catalyzed by fatty acyl-CoA synthetase and transported to various metabolic locations [ 11 ]. (biomedcentral.com)
  • Systems for the Cell-Free Synthesis of Proteins. (ezreregister.com)
  • However, large-scale protein dynamics datasets have remained scarce, due to the specialized technologies necessary to measure turnover of individual proteins on a global scale. (nature.com)
  • It has been previously shown that supplementing organotypic culture media with retinol improves the in vitro production of spermatids and spermatozoa in prepubertal mouse testicular tissues cultured at a gas-liquid interphase ( 5 , 7 ). (elifesciences.org)
  • Freshly isolated hepatocytes conserved MRP2/Mrp2 protein levels that are comparable with those in the liver tissues. (aspetjournals.org)
  • This technique allows non-destructive, label-free spectral imaging and analysis of cells, tissues, and nanoparticles [ 5 - 7 ]. (medsci.org)
  • How functionally important molecules, such as proteins and metabolites, contribute to these cell processes is largely unknown. (njacs.org)
  • The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
  • The biological role and underlying mechanism of action of zinc-finger protein 326 (ZNF326) in malignant tumors, including breast cancer, are still not clear. (cancerindex.org)
  • Manipulation of protein functions aids both biological research and disease treatments. (u-tokyo.ac.jp)
  • RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
  • The conjugate area has said biological drug detectably labeled and the capture area has said biological drug immobilized thereto. (justia.com)
  • Using a scalable plant expression system and reducing the in vitro deglycosylation burden creates the potential to eliminate glycan heterogeneity and provide affordable customization of therapeutics' glycosylation for maximal and targeted biological activity. (umd.edu)
  • Quantitative measurements derived from the MS technology can be translated into differential expression of proteins in biological samples, and collective molecular changes can then be used to describe complex phenotypes such as disease progression. (openaccesspub.org)
  • Key proteins in mitochondria and metabolic pathways are encompassed, in addition to contractile machineries and sarcolemmal signaling proteins. (nature.com)
  • Schizophrenia brain cells showed abnormal gene expression and protein levels related to cytoskeletal remodeling and oxidative stress. (ca.gov)
  • Consistent with recent reports indicating that neurons differentiated in vitro from human-induced pluripotent stem cells (hiPSCs) are immature relative to those in the human brain, gene expression comparisons of our hiPSC-derived neurons to the Allen BrainSpan Atlas indicate that they most resemble fetal brain tissue. (ca.gov)
  • From our findings that SZ hiPSC NPCs show abnormal gene expression and protein levels related to cytoskeletal remodeling and oxidative stress, we predicted, and subsequently observed, aberrant migration and increased oxidative stress in SZ hiPSC NPCs. (ca.gov)
  • Time and tissue specific gene expression patterns are manifested by the combination of DNA with proteins resulting in a structure called chromatin. (uni-goettingen.de)
  • All proteins were expressed in keratocytes cultured in all media in accordance to the gene expression findings. (blogspot.com)
  • The NcACBP recombinant protein was able to specifically bind acyl-CoA esters in vitro . (biomedcentral.com)
  • These processes are mediated by multi-modular proteins that bind to membranes in response to second messengers. (tamu.edu)
  • As a proof of principle, we applied this technique to yeast alcohol dehydrogenase-1 exposed in vitro to H 2 O 2 and also in vivo to the complex proteome of the Gram-negative bacterium Bacillus subtilis . (hindawi.com)
  • Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
  • c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
  • d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
  • The recombinant NcACBP protein was expressed to verify the binding activities of NcACBP in vitro , and the heterologous expression of NcACBP in Δacbp yeast in vivo . (biomedcentral.com)
  • Indeed, it has been demonstrated that cysteines are characterized by the most extreme conservation pattern, being highly conserved in functional positions of proteins but poorly conserved otherwise [ 5 ]. (hindawi.com)
  • We have identified a cytosolic acyl-CoA binding protein, with highly conserved amino acid residues and a typical acyl-CoA binding domain in N. caninum . (biomedcentral.com)
  • Interactions with other proteins or molecules are another feature of cysteines that can affect protein activity. (hindawi.com)
  • This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. (nih.gov)
  • Additionally, DBC1 knockdown eliminated the up-regulation of MMP7, EMT-related proteins, and cell cycle-related proteins as well as the enhanced proliferation and invasiveness induced by ZNF326. (cancerindex.org)
  • Here, I investigate the role of one intrinsic factor (receptor-type protein tyrosine phosphatase alpha) and one extrinsic factor (ionizing radiation) in facilitating cancer cell invasion. (ubc.ca)
  • Receptor-type protein tyrosine phosphatase alpha (PTPα is a widely expressed transmembrane-bound protein that has been implicated in integrin signaling, focal adhesion formation, and normal cell migration. (ubc.ca)
  • These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)
  • The critical difference between in vitro and in cell conditions sometimes leads to an unexpected difficulty, reducing the efficiency of the drug development process. (u-tokyo.ac.jp)
  • After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
  • As a new type of probe, the heavy isotope of water has been widely used in the study of cell development, metabolism, tissue homeostasis, aging, and tumor heterogeneity. (medsci.org)
  • As a new quantitative method, heavy water-labeled single-cell Raman microspectroscopy can reduce damage to cells and human interference factors. (medsci.org)
  • Co-translational stabilization of insoluble proteins in cell-free expression systems. (ezreregister.com)
  • Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins. (ezreregister.com)
  • 17.Rongrong Ma, Zhong Yang, Lei Huang, Xiangcheng Zhu, Lei Kai, Jin Cai, Xiaoning Wang, Zhinan Xu Construction of an efficient Escherichia coli cell-free system for in vitro expression of several kinds of proteins. (ezreregister.com)
  • Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. (blogspot.com)
  • When compared with the innovator product Rituxan®, the plant-made remodeled afucosylated antibody showed similar binding affinity to the CD20 antigen but significantly enhanced cell cytotoxicity in vitro. (umd.edu)
  • Here we develop and apply the principles of their evolution from systems principally for evaluating mechanism dosimetry in vitro and outline an approach for simulation of of toxicity to high-throughput systems for rapid and cost-effective nanoparticle particokinetics in cell culture systems. (cdc.gov)
  • To make this practical, mechanistic insights into the effects of THz radiation on proteins would be prerequisite. (u-tokyo.ac.jp)
  • The SARS-CoV-2 spike protein (S) is a prime example, having 22 potential sites of N-glycosylation per protein protomer, as predicted from the primary sequence. (cdc.gov)
  • In this report, we conducted mass spectrometric analysis of the N-glycosylation profiles of recombinant spike proteins derived from four common SARS-CoV-2 variants classified as Variant of Concern, including Alpha, Beta, Gamma, and Delta along with D614G variant spike as a control. (cdc.gov)
  • Our data reveal that the amino acid substitutions and deletions between variants impact the abundance and type of glycans on glycosylation sites of the spike protein. (cdc.gov)
  • Detailed site-specific glycan analysis of a wide variety of SARS-CoV-2 variants provides useful information toward the understanding of the role of protein glycosylation on viral protein structure and function and development of effective vaccines and therapeutics. (cdc.gov)
  • An efficient chemoenzymatic glycan remodeling technology using a plant expression system that combines in vivo deglycosylation with an in vitro chemoenzymatic glycosylation is described. (umd.edu)
  • Light-induced Printing of Protein Structures on Membranes in vitro. (ezreregister.com)
  • We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
  • Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
  • In vitro spermatogenesis could indeed be proposed to patients with testicular localization of residual tumor cells, for whom testicular tissue autografting is not indicated (about 30% of patients with acute leukemia). (elifesciences.org)
  • In Chapter 2, I examined how proteins secreted by cancer cells can promote a tumor-permissive microenvironment through the recruitment of suppressive innate and adaptive immune cells to the lungs. (ubc.ca)
  • I demonstrated that tumor cells secreted proteins that drove the recruitment and/or expansion of pulmonary TAMs, Tregs, and granulocytic-MDSCs (G-MDSCs), resulting in increased primary lung tumor growth and pulmonary metastasis of mammary carcinoma cells, respectively. (ubc.ca)
  • Targeting tumor-secreted proteins, or the suppressive immune cells that are expanded in response to said proteins, led to a decrease in primary and metastatic pulmonary disease. (ubc.ca)
  • I demonstrated that using glucose restriction of CD8⁺ T cells in vitro led to improved tumor regression in vivo. (ubc.ca)
  • We performed discovery-based genome-wide analysis of the RNA and proteins in control and schizophrenia brain cells, in order to better understand how they differ. (ca.gov)
  • By this approach we identified 37 up-regulated and 54 down-regulated proteins in BAG3-silenced cells. (oncotarget.com)
  • Trimethylation of histone H3 lysine 9 (H3K9me3) is one hallmark of heterochromatin, and is mediated by the methyltransferase Suv39 and recognized by heterochromatin protein 1 (HP1), which has three isoforms in mammalian cells. (uni-goettingen.de)
  • The isotope labeling strategy has been developed in E. coli expression systems, however, it is not that straight forwards in eukaryotic cells, such as insect cells and mammalian cells. (u-tokyo.ac.jp)
  • We have developed a strategy to selectively introduce methyl 13 C-labeling with the high-level deuteration of surrounding amino acid residues in insect cells. (u-tokyo.ac.jp)
  • Common culture formats used for in vitro enzyme induction studies comprise conventional and sandwich cultures, the former referring to plated hepatocytes maintained on rigid substratum (e.g., collagen), whereas in the latter cells are "sandwiched" by an additional layer of either collagen or a composite of extracellular matrix proteins, such as Matrigel ( LeCluyse, 2001 ). (aspetjournals.org)
  • Here, we review challenges and recent advances in the LC-MS-based analysis of minute protein amounts, down to the level of single cells. (biomed.news)
  • We report here a large dataset of protein turnover dynamics in the heart of six common genetic strains of mice, acquired under both normal and hypertrophic conditions. (nature.com)
  • The present dataset was collected using a technology platform we recently developed, which overcame several technical challenges in quantifying individual protein turnover rates on a proteome scale. (nature.com)
  • In a cross-sectional study of 48 AD patients without diabetes, 20 cognitively normal diabetic patients, 16 patients with frontotemporal dementia, and 84 cognitively normal controls, researchers found that dysfunctionally phosphorylated insulin receptor substrate-1 (IRS-1), a neuronal protein, is detectable in blood using exosome-based technology and may be able to predict AD up to 10 years before the appearance of symptoms. (medscape.com)
  • The effects of culture format (i.e., sandwich culture versus conventional culture) and of dexamethasone (DEX) media concentrations on mRNA, protein, and activity levels were determined for three donors, and protein expression was compared with that in liver. (aspetjournals.org)
  • The basic repeating element of chromatin is the nucleosome, where DNA is wrapped around an octamer of histone proteins, interconnected by sections of linker DNA. (uni-goettingen.de)
  • Conversely, chemical labeling, attaching small tags that carry isotope differences to the analytes, presents a simpler approach without the need to actually synthesize any peptides. (genengnews.com)
  • BAG3 protein is an apoptosis inhibitor and is highly expressed in Anaplastic Thyroid Cancer. (oncotarget.com)
  • Botulinum neurotoxins (BoNTs) are bacterial proteins that cause the life-threatening disease botulism 1 . (cdc.gov)
  • BoNTs are dichain proteins consisting of a light chain of 50 kDa and a heavy chain of 100 kDa linked by a disulfide bond 5 . (cdc.gov)
  • Many of these proteins are reportedly involved in tumor progression, invasiveness and resistance to therapies. (oncotarget.com)
  • Despite limited understanding on mechanisms, disruption of protein turnover is widely implicated in diverse pathologies from heart failure to neurodegenerations. (nature.com)
  • Reference materials are widely used for in vitro diagnostics to support the generation of reliable results and their correct use for diagnostic or prognostic purposes. (genengnews.com)
  • A significant lack of quantitative knowledge regarding non-Cytochrome P450 (non-CYP) enzymes and transporters in human is the major limitation towards building PBPK models in the drug development which often results inaccurate in vitro to in vivo extrapolation (IVIVE) and poor prediction of interindividual variability of drug metabolism. (njacs.org)
  • With a strict control for excluding thermal effects, we successfully recapitulated the non-thermal effects of THz radiation on the hydration on a protein surface ( Biophys J., 2021 ). (u-tokyo.ac.jp)
  • Folded proteins show a high degree of structural order and undergo (fairly constrained) collective motions related to their functions. (umd.edu)
  • On the other hand, intrinsically disordered proteins (IDPs), while lacking a well-defined three-dimensional structure, do exhibit some structural and dynamical ordering, but are less constrained in their motions than folded proteins. (umd.edu)
  • Regulation of proteome integrity requires chaperone-assisted folding of unfolded proteins, dissolution of misfolded aggregates, proteolytic removal of proteins, and other concerted proteostatic processes 1 , 2 . (nature.com)
  • The high diversity of proteins in any given proteome is reflected by a variety of workflows and study designs intended to achieve qualitative as well as quantitative coverage of the proteins. (genengnews.com)
  • We focused our attention on an oncogenic protein, CAV1, and a tumor suppressor protein, SERPINB2, that had not previously been reported to be modulated by BAG3. (oncotarget.com)
  • We previously reported that hepatobiliary transporter multidrug resistance-associated protein (MRP2/Mrp2) is considered to be the major cause of the interspecies differences detected by efflux of fluorescent substrates in isolated hepatocytes. (aspetjournals.org)
  • In comparison with our previously reported site-specific glycan analysis on the S-D614G and its ancestral protein, glycan types on later variants showed high similarity on the site-specific glycan content to S-D614G. (cdc.gov)
  • On the other hand, in vitro chemoenzymatic glycan remodeling has been successful in producing homogeneous pre-defined protein glycoforms, but needs to be combined with a cost-effective and scalable production method. (umd.edu)
  • Transient, reversible effects of the THz radiation to the protein structures were encoded in NMR signal intensities via proton exchange with solvent water. (u-tokyo.ac.jp)
  • The Glasner Lab investigates mechanisms of protein evolution in order to understand how the biophysical properties of proteins influence their capacity to evolve new structures and functions. (tamu.edu)
  • Application of electromagnetic waves at around terahertz (THz) frequency to proteins could be game-changing, as the THz radiation could modulate a protein function if it resonates with functionally relevant vibrational modes of the protein. (u-tokyo.ac.jp)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)