Vitro protein mutant proteins. Medical search. Frequent questions

In vivo virulence of the mutant viruses seems to decline in human patients, as assessed by fever duration in affected persons. (cdc.gov)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
Coincidently, we have discovered that microcystin binds to a number of these proteins in vivo . (uni-potsdam.de)
Endogenous protein substrates for in vitro and in vivo phosphorylation in cilia and ciliary membranes. (rupress.org)
The pattern of ciliary polypeptides labeled in vivo was similar to that observed in the in vitro experiments, although the relative intensities of labeling differed. (rupress.org)
Rbp38 is a multifunctional protein exerting functions in the kinetoplast, such as the stabilization of mitochondrial RNAs and in the nucleus, as a protein that binds in vivo kDNA, the double-stranded (LaTel) and the G-rich telomeric DNAs (Tel1). (unesp.br)
The growth and metastasis abilities of cells are evaluated in vitro and in vivo. (biomedcentral.com)
Recent unpublished results from the proposed host institution, the Department of Molecular Neurobiology (Dr. Klein) at Max Planck Institute of Neurobiology, indicate that shed ectodomains of FLRTs can function as long-range guidance cues for cortical neurons in vitro and in vivo. (europa.eu)

To clarify this issue, we decided to apply a transfection-based assay, named Host Cell Reactivation (HCR), to investigate DNA lesions removal efficacy in the presence of DDB2 Wt protein or DDB2 mutated one. (biomedcentral.com)
In vitro kinase assay is applied to identify the kinase of PIK3CA/p110α tyrosine phosphorylation. (biomedcentral.com)

A mutation that diminished Munc18 binding to syntaxin1 in vitro attenuated the vesicle-docking step but rescued vesicle priming in excess of docking. (jneurosci.org)
In vitro analyses of apoptosis through flow cytometry and confocal microscopy show that NANOS3 capacity to prevent apoptosis was impaired by this mutation. (hindawi.com)
The identification of an inactivating missense mutation in NANOS3 suggests a mechanism for POI involving increased primordial germ cells (PGCs) apoptosis during embryonic cell migration and highlights the importance of NANOS proteins in human ovarian biology. (hindawi.com)
Mutation of either region caused the protein to be more stable and mutation of both caused an additive effect, suggesting that both regions are important. (uri.edu)

In addition, the double mutant (Y42A/L48Q) of the PX domain of Vam7p, reported to cause vacuolar trafficking defects in yeast, has a dramatically decreased level of binding to PtdIns-3-P. These data reveal that the membrane targeting function of the Vam7p PX domain is based on its ability to associate with PtdIns-3-P, analogous to the function of FYVE domains. (embl.de)
The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)
The study, "Compromised catalysis and potential folding defects in in vitro studies of missense mutants associated with hereditary phosphoglucomutase 1 deficiency," was funded in part by the National Science Foundation and was published in The Journal of Biological Chemistry. (missouri.edu)
Six behavioral mutants of Paramecium, known to have defects in the excitable membrane that regulates the ciliary beat, showed normal patterns of ciliary protein phosphorylation in vitro, with and without added cyclic nucleotides, at both pH 6.0 and pH 8.0. (rupress.org)

Cell-fate-determinant molecule NUMB-interacting protein (TBC1D15) is overexpressed and contributes to p53 degradation in TICs. (nature.com)
suggesting protein misfolding and degradation. (jneurosci.org)
We identified two discrete regions of FtsZ important for degradation of both FtsZ monomers and polymers by ClpXP in vitro. (uri.edu)
We also observed that in vitro MinC inhibits degradation of FtsZ by ClpXP, suggesting that some of the same residues in the C-terminal site that are important for degradation by ClpXP are important for binding MinC. (uri.edu)

Inhibition of the Ras-MAPK pathway by expression of the dominant-negative Ras mutant HRasN17 or by addition of the MAPK kinase (MEK) inhibitor PD98059 reduced several of these parameters but failed to block transformation. (embl.de)
1976. The in vitro effect of zinc on the inhibition of human *-aminolevulinic acid dehydratase by lead. (cdc.gov)

The Paranoiac A mutant, however, showed a reduction in cyclic GMP-stimulated protein kinase activity. (rupress.org)
The second messenger PIP3 recruits and activates 3-phosphoinositide dependent protein kinase-1 (PDK1) and AKT serine/threonine kinase on the membrane [ 2 ]. (biomedcentral.com)
Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)

The mutated proteins can interact with normal proteins to form dysfunctional tetramers, and thus change the pattern of genome methylation ( 5 ). (frontiersin.org)
In addition, a mutant form unable to interact with PCNA (DDB2 PCNA- ), has shown a reduced ability to interact with a UV-damaged DNA plasmid in vitro. (biomedcentral.com)
The G174A substitution appears to impair the ability of a hydrophobic cleft in β to interact with the eubacterial clamp-binding motif (QL[S/D]LF) that is conserved in most partner proteins reported to interact with the β clamp ( 8 , 38 ). (asm.org)
In doing so, this GEF-facilitated exchange of GDP for GTP is associated with structural changes in the GTP-binding protein which influence the degree to which the GTP-binding protein can interact with other molecules. (justia.com)
When GTP is bound, for instance, Ras proteins can interact with effectors and other molecules to affect cell proliferation, differentiation and apoptosis. (justia.com)

The recruitment of specific cytosolic proteins to intracellular membranes through binding phosphorylated derivatives of phosphatidylinositol (PtdIns) controls such processes as endocytosis, regulated exocytosis, cytoskeletal organization, and cell signaling. (embl.de)
Thus, FLRT proteins have the potential to engage in bi-directional signaling ('forward' via Unc5 receptors and 'reverse' via their own intracellular domain), an original and innovative concept which Dr. Klein previously pioneered in the ephrin/Eph field. (europa.eu)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

The CLDN14 protein also binds to WW domain of Yes-associated protein. (wikipedia.org)
Structural analysis of the C-terminal domain reveals a dimer with a lysine-rich surface that binds DNA non-specifically and is essential for DNA condensation in vitro. (elifesciences.org)

ATP2A2, located on 12q23-24.1, encodes the sarcoplasmic/endoplasmic reticulum Ca 2+ -ATP isoform 2 protein (SERCA2), which is a calcium pump. (medscape.com)
The Escherichia coli dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. (asm.org)
The dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that bears two amino acid substitutions: G66E (glycine at position 66 replaced with glutamic acid) and G174A ( 14 , 35 , 38 ). (asm.org)
H ducreyi contains a fimbrialike protein (Flp) operon that encodes proteins that contribute to adherence and pathogenesis. (medscape.com)

The present thesis concerns studies of two α-amylases: 1) secondary substrate binding sites in barley α-amylase 1 (AMY1), and 2) the involvement of anti-nutrients in in vitro digestion of starch in legumes by porcine pancreatic α-amylase. (dtu.dk)
One substrate degraded by Escherichia coli ClpXP is FtsZ, an essential cell division protein. (uri.edu)
To better understand substrate selection by ClpXP, we engineered FtsZ mutant proteins containing amino acid substitutions or deletions near the FtsZ C-terminus. (uri.edu)

The protein encoded by CLDN14 is an integral membrane protein and a component of tight junctions, one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets. (wikipedia.org)
These junctions are composed of sets of continuous networking protein strands in the outer surface of the cell membrane, with complementary grooves in the inwardly facing extracytoplasmic leaflet. (wikipedia.org)
Protein modules such as FVYE domains and PH domains that bind specifically to PtdIns 3-phosphate (PtdIns-3-P) and polyphosphoinositides, respectively, can direct such membrane targeting. (embl.de)
Electron microscopy reveals loss of desmosomes (epithelial intercellular junctions formed by membrane and submembrane protein complexes), breakdown of desmosome-keratin intermediate filament attachment, and perinuclear aggregates of keratin intermediate filaments. (medscape.com)
Normally, upon the stimulations by growth factors (GFs), the receptor tyrosine kinases (RTKs) are activated, and then recruit PI3K protein complex on the membrane to generate phosphatidylinositol-3,4,5-triphosphate (PIP3) from phosphatidylinositol-4,5-biophosphate (PIP2) [ 2 ]. (biomedcentral.com)
Tyrosine phosphorylation of RTKs, or adaptor proteins such as IRS-1 facilitate their binding with p85 regulatory subunits of PI3K, and then bring PI3K complex on membrane [ 2 ]. (biomedcentral.com)
cSH2 domain S690 phosphorylation decreases p85 binding affinity to tyrosine-phosphorylated proteins resulting in less PI3K membrane localization [ 15 ]. (biomedcentral.com)
Lipid transport proteins at membrane contacts, where organelles are closely apposed, are critical in redistributing lipids from the endoplasmic reticulum (ER), where they are made, to other cellular membranes. (rupress.org)

Protein phosphorylation is crucial for signaling transduction of this pathway. (biomedcentral.com)

However, the use of mutant expression in the presence of endogenous protein left it unclear how the observed effects were related to the defect of vesicle docking in (M)unc18-1 knock-outs. (jneurosci.org)
The endogenous protein kinases of isolated Paramecium tetraurelia cilia phosphorylated approximately 30 ciliary polypeptides in vitro. (rupress.org)

Munc18-1 is a part of this fusion machinery, but its role is controversial because it is indispensable for fusion but also inhibits the assembly of purified SNAREs in vitro . (jneurosci.org)

Here, we combine in-vitro structural measurements, which used EPR spectroscopy and UV-Vis measurements, with cell experiments to explore the role of the various methionine residues in CusB. (biorxiv.org)

Substitution of Val 113 in Sendai virus (SeV) M protein generates non-functional polypeptides, characterized by their exclusion from virus particles and by their ability to interfere with virus particle production. (microbiologyresearch.org)
The present invention envisions phosphorylated forms of any one of these GEF-H1 peptides, polypeptides and proteins. (justia.com)

The substituted amino acid is located within the second C2HC motif in the conserved zinc finger domain of NANOS3 and in silico molecular modelling suggests destabilization of protein-RNA interaction. (hindawi.com)
Using its AFFITOME technology, AFFiRiS develops amino acid sequences that mimic the epitopes of self-proteins, which are modified by mutating the original amino acid sequence. (affiris.com)
These amino acid sequences, termed AFFITOPEs®, are coupled to a carrier protein and are formulated with an adjuvant to further enhance the immune response. (affiris.com)

The chemokine receptors CCR5 and CXCR4 belong to the class of 7 transmembrane G-protein-coupled receptors, and their natural ligands are key players in the recruitment of immune cells to sites of inflammation. (medscape.com)

Electrophoretic mobility shift assays (EMSA) were done in order to test the ability of each mutant to bind in vitro the G-rich telomeric DNA, double-stranded telomeric DNA and kDNA. (unesp.br)
The proposed fellow intends to tackle this problem using a multidisciplinary approach including conditional mouse mutant mice lacking either specific FLRT or Unc5 proteins, cell based functional assays using neurons derived from mutant mice, and molecular and biochemical assays to identify signalling pathways activated by FLRTs. (europa.eu)
In vitro assays and animal studies have provided information on the function of wild-type and variant GPCRs. (bvsalud.org)

Despite the detailed in vitro characterization of the enzymatic properties of yeast Sac1p, the exact cellular function of this protein has remained obscure. (embl.de)

Using the information provided by the NEJM study, we recreated the mutated proteins that cause the disorder in a test tube and conducted detailed biochemical analyses. (missouri.edu)
These mutant proteins were expressed in a bacterial system and were produced in high amounts as shown by SDS-PAGE and Western blotting analyses. (unesp.br)
Rare SOX10 variants were subjected to in silico and in vitro analyses. (bvsalud.org)

Previously we have shown that DDB2, a protein involved in the Global Genome Repair, interacts directly with PCNA and, in human cells, the loss of this interaction affects DNA repair machinery. (biomedcentral.com)
As a result, we have found clear evidence for a covalent interaction of the toxin with cysteines of proteins. (uni-potsdam.de)
Our results demonstrated that S1,RBD,N-IgY was able to inhibit RBD-ACE2 binding interaction in vitro, suggesting its potential use in blocking virus entry. (nutritruth.org)

Using the intrinsically fluorescent cholesterol analog, cholestatrienol, we directly observe sterol transport to mitochondria in fibroblasts upon treating NPC2 deficient human fibroblasts with NPC2 protein. (nature.com)
The production and secretion of 3 Flp proteins, Flp1, Flp2, Flp3, has been demonstrated to contribute to microcolony formation and attachment to human foreskin fibroblasts cells in vitro. (medscape.com)

Since Pol I is a multifunctional protein that participates in DNA replication, as well as numerous DNA repair pathways, several possibilities exist, including Okazaki fragment maturation ( 21 , 31 ) and single-stranded DNA (ssDNA) gap repair ( 16 , 22 ). (asm.org)

The factors and mechanisms regulating assembly of intermediate filament (IF) proteins to produce filaments with their characteristic 10 nm diameter are not fully understood. (johnshopkins.edu)

The in vitro starch digestion (hydrolytic index, HI) of pea (Pisum sativum) and mixtures of pea starch with different pea protein isolates and fiber has been investigated to learn about the involvement of anti-nutrients for the digestibility of legume starches. (dtu.dk)
Guanine nucleotide exchange factors ("GEFs") stimulate the dissociation of the GTP hydrolytic product, GDP, from small GTP-binding proteins, to promote the binding of a new GTP molecule. (justia.com)

And three, they chop up the mutant RNA, a process that is slow but appears to be effective in in vitro experiments. (illinois.edu)
The experiments show that oligomers form when the interdomain linker of one BiP molecule is bound to the region of an adjacent BiP molecule that is normally reserved for binding to unfolded proteins. (elifesciences.org)
Further experiments challenged cells with chemicals that caused the number of unfolded proteins in the cells to increase. (elifesciences.org)

The present work has the aim of mapping the boundaries of the DNA-binding domain of LaRBP38, since using the available in silico tools, we were not able to define any known nucleic acid binding domain in this protein. (unesp.br)

Lastly, we revealed that addition of type IV collagen protein induced myofibroblast proliferation and migration in monolayer culture and increased the formation of mesenchymal-epithelial septal-like structures in co-culture. (biomedcentral.com)
Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)
Here, live respiratory epithelial cells were inoculated with broth-grown or biofilm bacteria of different M-types, as well as with isogenic mutants lacking common virulence factors. (lu.se)
Dive into the research topics of 'Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)

[ 25 ] In particular, studies have revealed that keratosis follicularis (Darier disease) keratinocytes displayed abnormal trafficking of the desmosomal protein desmoplakin and abnormal expression of cytokeratins 10 and 14. (medscape.com)

When mutant DMPK is converted into RNA in a first step of protein production, the repeated sequences in the RNA cause it to bind to another protein, MBNL, which regulates RNA processing. (illinois.edu)

Previously, we reported the emergence and spread of mutant Middle East respiratory syndrome coronavirus bearing spike mutations (I529T or D510G) with reduced affinity to human receptor CD26 during the outbreak. (cdc.gov)
In previous studies, we reported the emergence and spread of mutant MERS-CoV bearing spike mutations (I529T or D510G) in receptor binding domain (RBD) with reduced affinity to human CD26 receptor during the South Korea outbreak ( 6 ). (cdc.gov)
The affinity to barley starch granules at SBS1 was 4-fold stronger than at SBS2, while the dual SBS1 and SBS2 mutants lost the ability to adsorb onto barley starch granules. (dtu.dk)

Two transcript variants encoding the same protein have been found for this gene. (wikipedia.org)
Here we report biallelic variants in CACNA2D1, encoding the α2δ-1 protein, in two unrelated individuals showing a developmental and epileptic encephalopathy (DEE). (stanford.edu)
The first study , published in January 2021, is titled: "Chicken Egg Yolk Antibodies (IgYs) block the binding of multiple SARS-CoV-2 spike protein variants to human ACE2. (nutritruth.org)

Regulator of G-protein signaling 5 regulates the shift from perivascular to parenchymal pericytes in the chronic phase after stroke. (lu.se)

We devise a protocol to determine the surface fraction of endo-lysosomes in contact with mitochondria and show that this fraction does not depend on functional NPC1 or NPC2 proteins. (nature.com)
The trypanosomatid Rbp38 does not share sequénce or functional/structural similarities with any other protein deposited in the public data bank. (unesp.br)

Legumes (beans, peas, and lentils) are characterised by low blood glucose raising potential, which is proportional to the in vitro starch digestion rates. (dtu.dk)

Mutant DMPK genes often continue to expand, amplifying the health problems that can result. (illinois.edu)

In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)
Our study was the first to systematically characterize and index these mutant proteins for comparison with the symptoms in human patients. (missouri.edu)
The AFFITOPE® formulation, the basis of SAIT, is administered to patients via a subcutaneous injection, which stimulates the generation of antibodies against the target proteins. (affiris.com)
ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)

Many proteins are made in a compartment within the cell called the endoplasmic reticulum and 'chaperone' proteins help them fold correctly. (elifesciences.org)
This leads to an increase in the number of unfolded proteins in the endoplasmic reticulum and so the cell increases the levels of chaperone proteins to cope with this. (elifesciences.org)
used biochemical techniques to study how an Hsp70 protein in the endoplasmic reticulum called BiP forms oligomers. (elifesciences.org)

[ 8 ] In a small human trial, injection of a deletion mutant that lacked expression of all 3 Flp proteins into volunteers demonstrated significantly reduced size of papule formation, as well as pustule formation rate. (medscape.com)

The most potent compounds the researchers developed reduce levels of the mutant RNA in cells that replicate the pathology of DM1. (illinois.edu)
After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
The results have shown that DDB2 Wt recognize and repair the UV-induced lesions in plasmidic DNA transfected in the cells, whereas a delay in these processes were observed in the presence of DDB2 PCNA- , as also confirmed by the different extent of co-localization of DDB2 Wt and some NER proteins (such as XPG), vs the DDB2 mutant form. (biomedcentral.com)
Cells carefully regulate the levels of chaperone proteins. (elifesciences.org)
However, when the levels of unfolded proteins rise, cells can rapidly break up these oligomers to make active monomers that help to deal with the excess numbers of unfolded proteins. (elifesciences.org)
Further work is needed to understand how changes in the number of unfolded proteins in cells leads to the formation and disassembly of BiP oligomers. (elifesciences.org)
The M3 protein, but not the M1 or M5 proteins, was required for optimal uptake and persistence of both planktonic and biofilm bacteria inside cells. (lu.se)

The microcystin deficient mutant mcyB showed significant changes in the accumulation of proteins, including several enzymes of the Calvin cycle, phycobiliproteins and two NADPH-dependent reductases. (uni-potsdam.de)
The nature of the binding was studied using extracts of a microcystin-deficient mutant in vitro . (uni-potsdam.de)

Wild type (wt) mouse NF-L formed 10 nm filaments in assembly buffer containing 175 mM NaCl, whereas a mutant deleted of 18 NH 2 -terminal amino acids failed to assemble under similar conditions. (johnshopkins.edu)
Our results suggests that the known inability of nuclear lamin proteins to assemble into 10 nm filaments in vitro cannot derive solely from their longer rod domain. (johnshopkins.edu)

These enzymes perform the same chemical reaction as the human protein involved in the newly identified inherited disease and share many other similarities. (missouri.edu)
AFFiRiS is a clinical-stage biotechnology company located in Vienna, Austria, with a vision of using the immune system to identify and target human proteins central to the development and progression of chronic diseases, based on its proprietary patented AFFITOME® technology. (affiris.com)

Such protein-mediated transfer is especially important for maintaining organelles disconnected from secretory pathways, like mitochondria. (rupress.org)

RNA-seq results showed that transcription factors and macrophage inflammatory proteins were significantly downregulated in the DNMT3A mutant clones. (frontiersin.org)
Crispr/CAS9 strategy is applied to generate Y317F and Y508F mutant knock-in cell clones. (biomedcentral.com)

The RNA-binding protein NANOS3 poses as an interesting candidate gene for POI since members of the Nanos family have an evolutionarily conserved function in germ cell development and maintenance by repressing apoptosis. (hindawi.com)
For example, the cell translates the mutant RNA into proteins that also turn out to be toxic. (illinois.edu)
We have further discovered a role of microcystin as infochemical and a strong influence of the toxin on the expression of cell surface proteins. (uni-potsdam.de)
Taken together, our data suggest a redox-dependent, protein-modulating role for microcystin within the producing cell, which represents a new addition to the catalogue of functions that have been discussed for microbial secondary metabolites. (uni-potsdam.de)
If there are too few chaperones in the cell, then newly-made proteins may fold incorrectly and interrupt other processes. (elifesciences.org)
If a cell experiences stressful conditions, or if there is a sudden demand for more proteins to be made, protein folding can be disrupted. (elifesciences.org)
These proteins can be present in a cell as single molecules (monomers) or as a group of several chaperone molecules (oligomers). (elifesciences.org)
Previous research has suggested that the chaperone proteins in oligomers are inactive, but the oligomers may be rapidly broken down into monomers when the cell needs to fold more proteins. (elifesciences.org)
The present project aims to study the role and mechanism of action of the Fibronectin Leucine-Rich Transmembrane (FLRT) family protein in axon guidance and cell migration during mouse central nervous system (CNS) development. (europa.eu)
We have established a method allowing to study the cell-specific secretome of pericytes in vitro-using Turbo-ID, a mutant non-toxic biotin ligase (6). (lu.se)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
protein patterns of subjects with leish- Cell culture maniasis [17]. (who.int)

Exocytosis of secretory or synaptic vesicles is executed by a mechanism including the SNARE (soluble N -ethylmaleimide-sensitive factor attachment protein receptor) proteins. (jneurosci.org)

The p53 interacting partner protein NUMB (homology of numb protein) preserves this intrinsic cellular asymmetry by preventing ubiquitin-mediated proteolysis of p53 catalyzed by the MDM2 E3 ubiquitin ligase 11 . (nature.com)

While GG-NER employs UV-DDB heterodimer and XPC complex to initiate the DNA repair process, TC-NER utilizes elongating RNA polymerase II (Pol II) and Cockayne syndrome B (CSB) proteins as damage sensors [ 8 ]. (biomedcentral.com)
These phenotypes appear to result, at least in part, from impaired interactions of the mutant β159 clamp protein with the replicative DNA polymerase, Pol III ( 38 ). (asm.org)

Members of a subfamily of these enzymes share a specific domain that was first identified in the yeast Sac1 protein [1]. (embl.de)

In particular, the relationship between these dynamic nucleoprotein complexes and the molecular architecture of the ParB protein is unclear and is the subject of the work presented here. (elifesciences.org)

Claudin-14 is a protein that in humans is encoded by the CLDN14 gene. (wikipedia.org)
Analysis of the Sendai virus M gene and protein. (microbiologyresearch.org)

We identify mitoguardin-2, a mitochondrial protein at contacts with the ER and/or lipid droplets (LDs), as a lipid transporter. (rupress.org)

Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
Most of the phosphopolypeptides labeled in vitro also turned over rapidly in vitro. (rupress.org)

The other region is near the FtsZ C-terminus and partially overlaps the recognition sites for several other FtsZ-interacting proteins, including MinC, ZipA and FtsA. (uri.edu)

d - i Type IV collagen protein and mRNA expression in the lung. (biomedcentral.com)

O presente trabalho teve por objetivo mapear os domínios de interação da proteína LaRbp38 (RNA binding protein 38 KD) de Leishmania amazonensis com ácidos nucléicos e a identificação de proteínas parceiras que formassem possíveis complexos com ela nos telômeros do parasita. (unesp.br)
Recent results demonstrated that Rbp38 is a protein exclusively expressed in trypanosomatid parasites, which includes the genus Leishmania. (unesp.br)

Transgenic animal models expressing rhodopsin glycosylation mutants also exhibit light exacerbated retinal degeneration (RD). In this study, we used transgenic Xenopus laevis to investigate the pathogenic mechanism connecting light exposure and RD in photoreceptors expressing T4K or T17M rhodopsin. (jneurosci.org)

In addition, we demonstrate that the β clamp stimulates the processivity of Pol I in vitro and that β159 is impaired for this activity. (asm.org)
On the path to achieving this, we have developed SAIT, a novel therapy that stimulates the body to find and fight proteins that are central to the development and progression of these diseases. (affiris.com)

They undergo activation, morphological changes and express a protein called Regulator of G-protein Signaling 5 (RGS5) (1, 2). (lu.se)

When bound by the mutant RNA, MBNL cannot function properly, causing a cascade of problems in protein production, Zimmerman said. (illinois.edu)

In addition, neutralizing activity against these 2 mutant viruses in serum samples from mice immunized with wild-type spike antigen were gradually reduced, suggesting emergence and wide spread of neutralization escapers during the outbreak. (cdc.gov)
Our results show that the anti-Spike-S1 IgYs showed significant neutralizing potency against SARS-CoV-2 pseudovirus, various spike protein mutants, and even SARS-CoV in vitro. (nutritruth.org)

Here we identify valosin-containing protein (VCP) as a regulator of p53-R273H by conducting immunoprecipitation-tandem mass spectrometry analysis. (aacrjournals.org)
Berger M, Bergers G, Arnold B, Hammerling GJ, and Ganss R. Regulator of G-protein signaling-5 induction in pericytes coincides with active vessel remodeling during neovascularization. (lu.se)
Loss of Regulator of G-Protein Signaling 5 Leads to Neurovascular Protection in Stroke. (lu.se)

We also demonstrate that lengthening of the NF-L rod domain, by inserting a 42 aa sequence unique to nuclear IF proteins, does not compromise filament assembly in any noticeable way. (johnshopkins.edu)
Other controversial point about LaRbp38 is whether this protein has nuclear and mitochrondrial subcelular localization. (unesp.br)

Such mutants constitute potential tools for the identification of critical steps in paramyxovirus assembly and budding. (microbiologyresearch.org)

The mechanisms by which specific ATP2A2 mutations impact the function of the ATP2A2 protein have been investigated using an in vitro model. (medscape.com)
ClpXP is a two-component ATP-dependent protease that unfolds and degrades proteins bearing specific recognition signals. (uri.edu)
Background Mutations in forkhead box protein P1 ( FOXP1 ) cause intellectual disability (ID) and specific language impairment (SLI), with or without autistic features (MIM: 613670). (bmj.com)

Proteins are composed of long chains of amino acids that fold on themselves to form three-dimensional structures. (elifesciences.org)

Sendai virus M protein is found in two distinct isoforms defined by monoclonal antibodies. (microbiologyresearch.org)

Rhodopsin consists of a protein (rod opsin) and a chromophore ligand (11- cis -retinal) and initiates phototransduction in a specialized organelle called the rod outer segment (OS). (jneurosci.org)

Y105A/Y380A and Y105A/Y380M showed decreased activity (kcat) on amylose, reminiscent of single SBS2 mutant proteins, Y380A and Y380M, and KM increased approx. (dtu.dk)
We evaluated FOXP1 activity by an in vitro luciferase model and assessed protein stability in vitro by western blotting. (bmj.com)

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