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  • cryopreservation
  • In dogs his work studies peri-ovulatory physiology, cryopreservation of gametes, sperm transport and sperm-oocyte interaction. (nottingham.ac.uk)
  • Vitrification is widely used in embryo and oocyte cryopreservation, but its effectiveness for ovarian freezing remains debatable. (publish.csiro.au)
  • However, in addition to in situ conservation, methods or techniques to maintain live animals outside their production or natural environment (ex situ live) or through cryopreservation of germplasm (ex situ) are set up to preserve (germplasm of) rare breeds as well as the more widely used commercial breeds. (fao.org)
  • To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. (biomedcentral.com)
  • The findings of differential expressed proteins may contribute to effective ways of proteome analysis of oocyte/embryo quality in order to assess safety of cryopreservation in felid species. (biomedcentral.com)
  • To date, cryopreservation of oocytes is an essential part in reproductive biotechnology, and is an interesting procedure to conserve female gametes. (biomedcentral.com)
  • Thus, the purpose of the present study is to identify the alterations of protein expression after cryopreservation of domestic cat oocytes. (biomedcentral.com)
  • In Vitro Embryo Production (IVEP) and Embryo Cryopreservation which generated successful pregnancies and live births both in the institutional and village herds. (gov.ph)
  • sperm
  • Spermatozoa were acquired by microepididymal sperm aspiration and processed using swim-up technique. (springer.com)
  • Intracytoplasmic sperm injection was performed on mature oocytes after 2 h of incubation and on in vitro matured (IVM) oocytes 4 h after maturation. (springer.com)
  • As continuation of our previous post on sperm selection, we revisit the topic from the perspective of the numerous techniques currently available to be specifically used prior intracytoplasmic sperm injection (ICSI) . (weebly.com)
  • Several techniques are currently utilised for this purpose, each of them relying on different features in order to select the optimal sperm. (weebly.com)
  • Volume 1 (Sperm and Oocyte Analysis) effectively describes how to manipulate mature germ cells, sperm and oocytes, from diverse organisms such as ascidians and pigs. (biologists.org)
  • artificial
  • Madrid, Spain: Scientists believe that they are an important step nearer to success in creating an artificial egg from the combination of the nucleus of a somatic cell and an oocyte which has had its DNA-carrying nucleus removed, a conference of international fertility experts heard today (Tuesday 1 July). (innovations-report.com)
  • As a result of an artificial activation, a second round of chromosome segregation provides the haploid (23 chromosome) normal oocyte content. (innovations-report.com)
  • In oocytes from hibernation-interrupted toads, the p34(cdc2) protein level was significantly lower than in oocytes from toads in artificial hibernation, providing an explanation for the different quantities isolated by recombinant tUCHL1 pull-down and, more importantly, identifying a mechanism involved in the toad oocytes dependence on a low environmental temperature during winter hibernation. (jove.com)
  • meiotic
  • This dataset provides a unique reference resource for studies concerned with the molecular mechanisms controlling oocyte meiotic maturation in cattle, addresses the existing conflicting issue of transcription during meiotic maturation and contributes to the global goal of improving assisted reproductive technology. (springer.com)
  • produced by somatic cell nuc
  • The authors conclude that although in this early phase of research no direct evidence can be provided about the practical use of transgenic pigs produced by somatic cell nuclear transfer as organ donors or disease models, the future chances even in medium term are good, and at least proportional with the efforts and sums that are invested into this research area worldwide. (publish.csiro.au)
  • 2007. Birth of viable female dogs produced by somatic cell nuclear transfer. (koreascience.or.kr)
  • Microinjection
  • Although microinjection provides a direct method for delivery of nucleic acid for cells that are difficult to transfect, it is low-throughput and a technique that is difficult to master. (mirusbio.com)
  • methods
  • In addition to the review of literature (Chapter 1), general materials and methods (Chapter 2) and general discussion (Chapter 6), in Chapter 3 and 4, the studies focused on optimizing electrical parameters on pig oocyte activation and investigating the effects of activation conditions including temperature, activation medium, and concentrations of Ca 2+ and Mg 2+ in activation medium and diploidization of activated oocytes. (bl.uk)
  • Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. (stanford.edu)
  • reproductive
  • The objective of this research study is to evaluate a newly developed oocyte maturation media system for human clinical use in assisted reproductive technology (ART). (clinicaltrials.gov)
  • A significant association was observed between the reproductive phases and oocyte quality (p=0.002). (vin.com)
  • Dr Peter Nagy, from Reproductive Biology Associates, Atlanta, collaborating with the University of Connecticut, USA, told the European Society of Human Reproduction and Embryology annual conference that former approaches to haploidisation using a fully mature oocyte and a resting (interphase) somatic cell had caused misaligned chromosomes during cell division. (innovations-report.com)
  • Meanwhile, the potential of reproductive techniques in harnessing female germplasm was seriously pursued by the PCC's Reproductive Biotechnology Unit during the past several years. (gov.ph)
  • More recent reproductive technology applied in buffalo reproduction is the Ultrasound-Guided Transvaginal Oocyte Retrieval, also known as Ovum Pick-UP (OPU). (gov.ph)
  • mature
  • Mature oocytes had been used in 500 l of TL-HEPES supplemented with Belinostat 0.1% hyaluronidase and were freed of cumulus cells by mechanical pipetting. (stemcellethics.net)
  • Recombinant tUCHL1 absorbed p34(cdc2), a component of MPF, in obviously larger quantities from mature oocytes than from immature oocytes, and p13(suc1) was isolated from tUCHL1 with a dependence on the ATP regeneration system, suggesting that still other functions may be involved in their association that require phosphorylation. (jove.com)
  • CONCLUSION
  • Conclusion: "VOP" HD closure reinforced by mesh introduced wihtout P10 and suturing with spinal needle technique is better for anatomical continuity, reduction of sac space, overlap with smaller mesh fixed by atraumatic sutures ensuring scientific application of Pascal's law and eliminating PSH. (blogs.com)
  • ultrastructural
  • The oocytes were collected as described in study I. After appropriate slide fixation and preparation, the material was analyzed for morphological and ultrastructural characteristics and photographed. (vin.com)
  • No ultrastructural differences were seen in oocytes of adult and young cats. (vin.com)
  • donor
  • Theoretically, among various possibilities, an established somatic cell nuclear transfer system with genetically engineered donor cells seems to be an efficient and reliable approach to achieve this goal. (publish.csiro.au)
  • Bovine hearing epidermis fibroblast (bESF) cells had been utilized as donor cells for nuclear transfer. (stemcellethics.net)
  • 2003. Epigenetic characteristics of bovine donor cells for nuclear transfer: Levels of histone acetylation. (koreascience.or.kr)