VivoProliferationTranswell MigrationFlow cytometryInvasion assaysAngiogenesisScratch assaysChamber assayLymphocyteEndothelialMetastasisCellsViabilityLuciferaseImmunohistochemicalChemotaxisCellularBiologicalAssessPivotalQuantificationWoundELISANeuralPutativeMechanismsImmunofluorescenceImmunohistochemistryExtracellularCell behaviorOverexpressionAdhesionMacrophagesCompoundsTissuesCancer ResearchMoleculesInhibitionClinicalMethodsFindingsResultsFormationGlioma
Vivo26
- The predictions were confirmed using a functional in vitro assay (scratch assay) as well as an ex vivo multicellular environment (aortic ring angiogenesis assay), validating the concept that endothelial cells can be used as an effective screening tool of exposed workers for determining bioactivity of altered circulatory factors. (cdc.gov)
- Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
- In vitro and in vivo results showed that POGLUT2 knockdown could delay tumor growth and progression. (frontiersin.org)
- The focus here is placed on developing, evaluating, validating and utilizing tailored in vitro and in vivo models. (fraunhofer.de)
- To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
- CCDC43 was necessary for FOXK1- mediated EMT and metastasis in vitro and vivo. (karger.com)
- Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
- We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
- I demonstrated that using glucose restriction of CD8⁺ T cells in vitro led to improved tumor regression in vivo. (ubc.ca)
- My work involving the depletion of PTPα in TNBC reveals PTPα as a regulator of ECM degradation and invasion in vitro and in vivo. (ubc.ca)
- Area A will delineate the diagnostic potential of TSPO imaging by means of positron emission tomography (PET) in brain tumors in vivo and will enable the complementary characterization of brain tissue in vitro to delineate the neurobiological characteristics underlying TSPO PET labeling in the human brain. (uni-regensburg.de)
- The biological functions of PKM2 were investigated in vitro and in vivo . (researchsquare.com)
- Silencing PKM2 markedly inhibits proliferation and metastasis capacity in vivo and in vitro , and vice versa. (researchsquare.com)
- Today, several in vivo and in vitro testing methods are available to assess the performance of skin care products and ingredients on biomechanical properties of the skin. (premiumbeautynews.com)
- The in-vitro or ex-vivo assays can target the various biological mechanisms providing a limitless opportunities of claims support. (premiumbeautynews.com)
- In this study, we investigated HIF-2α expression and VM by immunohistochemistry in 70 pancreatic cancer patients as well as the role of Twist1and Twist2 in HIF-2α-induced VM in vitro and in vivo . (oncotarget.com)
- The effects of PIP5K1α and its inhibitor on growth and invasion of BC were confirmed by using in vitro assays including proliferation, migration, apoptosis and luciferase reporter assays and in vivo xenograft mouse models. (lu.se)
- Among them, in vitro assays have been quantitative and expeditious as initial approximations that warrant further confirmation in vivo . (news-medical.net)
- A majority of endothelial cell assays utilize human umbilical vein endothelial cells or bovine aortic endothelial cells - not because these types of cells are good representatives of vascular conditions in vivo , but primarily because they are relatively easy to harvest from large blood vessels. (news-medical.net)
- Furthermore, in vivo interaction between endothelial cells and other cells types (such as fibroblasts, pericytes and macrophages) is cumbersome to translate and simulate in vitro . (news-medical.net)
- PKM2 knockdown inhibited cell migration and invasion, reduced SOD2 (manganese superoxide dismutase) activity and the intracellular H 2 O 2 level, and inhibited tumour growth and lung metastasis in vivo . (oncotarget.com)
- Immunosuppression mediated by MICCop was investigated in vivo by daily assessment of clinical signs of paralysis and in in vitro restimulation assays of peripheral immune cells. (uni-koeln.de)
- In this review, we discuss the various factors of BNNT production that can influence its toxicity followed by summarizing the toxicity findings from in vitro and in vivo studies conducted to date, including a review of particle clearance observed with various exposure routes. (cdc.gov)
- This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
- Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
- This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. (lu.se)
Proliferation18
- In vitro assays confirmed that the two compounds, to varying extents, inhibited RA-FLS proliferation, migration, invasion, and NF-κB signaling. (frontiersin.org)
- Proliferation and cell viability were assayed by means of the enzymatic cleavage of the tetrazolium salt WST-1 to formazan dye by cellular mitochondrial dehydrogenases. (scirp.org)
- In addition, the impact of miR-126 on cell proliferation, apoptosis, migration and invasion was detected in ESCC cell lines. (spandidos-publications.com)
- miR-126 mimics downregulated the expression of IRS-1 and GOLPH3 protein and suppressed the proliferation, migration and invasion of ESCC cells, whereas miR-126 inhibitors led to the opposite results. (spandidos-publications.com)
- miR-126 suppressed the proliferation, migration and invasion of ESCC cells, and acted as a tumor suppressor in the carcinogenesis of ESCC. (spandidos-publications.com)
- Moreover, in vitro assays revealed that the decreased expression of lncRNA ANRIL could suppress the cell proliferation, migration and invasion HCC cells. (nih.gov)
- Cell viability and proliferation assays can be effectively used to evaluate cell health. (sigmaaldrich.com)
- For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
- Interference with and overproduction of GRP78 in vitro impacted the proliferation, migration, invasion, and metastasis of HCC cells. (bvsalud.org)
- The ECM composed of hundred proteins is a dynamic network controlling the proliferation, adhesion, migration, polarity, differentiation, and apoptosis of the cells. (premiumbeautynews.com)
- Various assays were used to investigate the stimulatory effects of the purified rFg14-3-3e protein on specific functions of goat PBMCs, including cytokine secretion, proliferation, migration, nitric oxide (NO) production, phagocytosis, and apoptotic capabilities. (biomedcentral.com)
- Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)
- Traditionally scratch assays have been used to study cell migration, cell proliferation and wound healing. (2bscientific.com)
- Cells may then be treated or monitored for proliferation or migration across the wound field by imaging samples at fixed time points or by time-lapse microscopy. (2bscientific.com)
- Those are degradation of adjacent matrix, migration toward the angiogenic stimuli, proliferation to create additional cells for the new blood vessels, as well as reorganization to form 3D vessel structures. (news-medical.net)
- There are a myriad of well-established in vitro methods for studying cell proliferation. (news-medical.net)
- The most reliable among them calculates proliferation by direct measurement of DNA synthesis, and thymidine incorporation assay is often cited as a reference model. (news-medical.net)
- Other in vitro angiogenesis assays that cover all angiogenic functions ( i.e. proliferation, migration, tube formation) include mouse metatarsal assay, embryoid assay and others. (news-medical.net)
Transwell Migration3
- Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. (sigmaaldrich.com)
- Using in vitro transwell migration and invasion assays, the effect of anthocyanins and possible metabolites on processes such as extravasation, cell adhesion molecules (integrins and selectins) and proteolytic enzymes (matrix metalloproteinases (MMP)) are investigated. (uni-giessen.de)
- The transwell migration assay (a modification of the classical Boyden chamber assay) is frequently utilized to assess endothelial cell migration, which is one of the main in vitro assays in the repertoire of Thermo Fisher Scientific. (news-medical.net)
Flow cytometry2
- The number and inhibitory activity of CD4(+)CD25(+)FoxP3(+) regulatory T cells were analysed by histology, flow cytometry and in vitro mixed lymphocyte cultures. (uni-koeln.de)
- METHODS: Cell Counting Kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), colony formation assays, flow cytometry, and transwell invasion assay were employed to determine cell growth, the apoptotic rate, cell invasion of NSCLC cells, respectively. (bvsalud.org)
Invasion assays1
- Explore tumor metastasis employing various cell migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay and in vitro scratch assay. (sigmaaldrich.com)
Angiogenesis7
- Changes in relative mRNA patterns indicated that serum from rats exposed to MMA-SS, and not GMA-MS or PBS, could influence several functional aspects related to endothelial cells, including cell migration, angiogenesis, inflammation, and vascular function. (cdc.gov)
- Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology. (sigmaaldrich.com)
- Angiogenesis assays can be used to study both the induction and inhibition of angiogenesis in in vitro cell models. (sigmaaldrich.com)
- Cell-based assay development for drug discovery can accelerate optimization of treatments for cancer and other angiogenesis-dependent diseases. (sigmaaldrich.com)
- The development of angiogenesis assays was a pivotal step in the discovery of proangiogenic and antiangiogenic molecules. (news-medical.net)
- Therefore in vitro angiogenesis assays should be viewed as a starting point rather than an endpoint for discovery. (news-medical.net)
- The aortic ring angiogenesis assay is widely used in angiogenesis research due to its reliability and reproducibility. (news-medical.net)
Scratch assays1
- Current quantification methods of scratch assays deal poorly with irregular cell-free areas and crooked leading edges which are features typically present in the experimental data. (ox.ac.uk)
Chamber assay1
- Cell migration and invasion can be evaluated in vitro with widely-used membrane-based migration systems such as the Boyden Chamber assay. (sigmaaldrich.com)
Lymphocyte2
- The relationship of in vitro lymphocyte defects to clinical infections is not understood. (medscape.com)
- 4 ]. A relationship between activated or lymphocytes and enzyme-linked immuno- memory-effector lymphocyte populations sorbent assay (ELISA) for CD117. (who.int)
Endothelial6
- Simoneau, B., Houle, F. and Huot, J. (2012) Regulation of Endothelial Permeability and Transendothelial Migration of Cancer Cells by Tropomyosin-1 Phosphorylation. (scirp.org)
- It has been reported that miR-126 is located on chromosome 9q34.3 within the host gene encoding for epidermal growth factor like-7 (EGFL-7), an endothelial cell-derived, secreted inhibitor of smooth muscle cell migration and a regulator of blood vessel formation ( 12 , 13 ). (spandidos-publications.com)
- In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
- To study the migration of microglia across the BBB, we developed an in vitro co-culture system of mouse brain endothelial cells (MBECs) and Ra2 microglia using Transwell inserts. (portlandpress.com)
- In conclusion, we offer evidence that microglia migration across the brain endothelial cell monolayer is increased in the presence of ATP in a manner that involves MMP secretion. (portlandpress.com)
- At the moment, the most popular tube formation assays involve plating human umbilical vein endothelial cells or bovine aortic endothelial cells with Matrigel. (news-medical.net)
Metastasis1
- Within this project, we hypothesize that anthocyanins and their metabolites inhibit cancer cell migration, which is a key property of cancer metastasis and inflammation. (uni-giessen.de)
Cells10
- In the present study, the effect of miR-183 on cell viability, cell cycle progression, and migration was investigated, and the interaction between miR-183 and PTEN in BC cells was examined. (nature.com)
- Adhesion assays were performed at various periods of time on cells grown on plastic. (scirp.org)
- Cell migration was evaluated by using the wound-healing assay and by measuring transendothelial migration of cancer cells. (scirp.org)
- Similarly, the transendothelial migration of MDA-MB231/S283E/Tm1 cells is also reduced as compared to the other cell lines. (scirp.org)
- Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. (scielo.br)
- These assays may be used to quantify and to assess the health of mammalian cell lines, primary cells and stem cells. (sigmaaldrich.com)
- Cell migration is the movement of cells in response to specific external signals, including chemical and mechanical stimuli. (sigmaaldrich.com)
- In the chemotaxis migration assay, pretreatment with fluvastatin and simvastatin inhibited the migration of human CD4+ T cells towards CCL20. (nih.gov)
- During normal cell migration, cells use focal adhesions to facilitate cycles of cell adhesion to, and release from, the extracellular matrix (ECM). (ubc.ca)
- Migration of MICCop cells after injection was examined by biodistribution analysis of (111)Indium-labelled MICCop. (uni-koeln.de)
Viability2
- To determine the impacts of miR-183 on cell viability, the MTT assay was used. (nature.com)
- MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. (nature.com)
Luciferase1
- PTEN was validated by dual luciferase assay as a target gene of miR-183. (nature.com)
Immunohistochemical1
- Histological and immunohistochemical analysis showed keratinocyte migration at 2 days following wounding. (mattek.com)
Chemotaxis1
- In leukocyte adhesion deficiency I, assays of random migration, chemotaxis, phagocytosis, and killing by neutrophils invariably show deficits in these functions. (medscape.com)
Cellular6
- Finally, we provide evidence that cellular phenotypes associated with autism-linked gene haploinsufficiency can be rescued by transcriptional activation of the intact allele in vitro, offering a proof of concept for a potential therapeutic approach for ASDs. (nature.com)
- The scratch assay is an in vitro technique used to assess the contribution of molecular and cellular mechanisms to cell migration. (ox.ac.uk)
- Cellular assays, or cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. (sigmaaldrich.com)
- Our data suggest that rFg14-3-3e protein can influence various cellular and immunological functions of goat PBMCs in vitro and may be involved in mediating F. gigantica pathogenesis. (biomedcentral.com)
- During their migration and development, liver flukes encounter various host tissue types, distinct physiological microenvironments, and a combination of both humoral and cellular immune responses. (biomedcentral.com)
- Humoral and cellular immune responses were then determined by ELISA and in vitro antigen restimulation assay. (uni-koeln.de)
Biological1
- Cell migration plays a critical role in a number of biological processes such as embryonic development, wound healing, and immune response. (clarkson.edu)
Assess2
- Consequently, we aimed to assess miR-637 expression in gliomas to determine the effects of miR-637 on glioma cell migration and invasion, as well as to explore the possible signaling pathways involved. (scirp.org)
- Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and can assess compound characteristics simultaneously. (sigmaaldrich.com)
Pivotal1
- Altogether, our analysis highlights a pivotal role for Cul3 in brain development, identifies a new player of neuronal migration, and provides a proof of concept of CRISPR-mediated rescue of an ASD-linked genetic defect. (nature.com)
Quantification2
- We introduce a new migration quantification method, called 'monolayer edge velocimetry', that permits analysis of low-quality experimental data and better statistical classification of migration rates than standard quantification methods. (ox.ac.uk)
- Application of this new method will enable quantification of migration rates from in vitro scratch assay data that cannot be analysed using existing methods. (ox.ac.uk)
Wound8
- To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. (nature.com)
- Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)
- However, these assays lack a consistently defined wound gap and can result in high inter-sample variation. (2bscientific.com)
- Our CytoSelect™ 24-Well Wound Healing Assay provides a much more consistent method to measure cell migration across a wound field gap in vitro. (2bscientific.com)
- Other variants of migration assays include under-agarose assay, Teflon fence assay, wound healing assay and phagokinetic track assay. (news-medical.net)
- A model of wound healing was created by introducing epidermal wounds in a full thickness in vitro human skin model (EpiDermFT) using a 3-mm punch biopsy and subsequently evaluated at multiple recovery time points. (mattek.com)
- Historically, EpiDermFT has been used to evaluate re-epithelization of the wound by a) manually bisecting the tissues through the center of the wound, b) staining with hematoxylin and eosin, and c) quantifying migration from the wound origin. (mattek.com)
- Accurate bisection of the wound is difficult and often leads to variability in assay results. (mattek.com)
ELISA1
- Cytokine profiling was performed by enzyme-linked immunosorbent assay (ELISA). (uni-koeln.de)
Neural4
- At the molecular level, we found that Cul3 controls neuronal migration by tightly regulating the amount of Plastin3 (Pls3), a previously unrecognized player of neural migration. (nature.com)
- Furthermore, we found that Pls3 cell-autonomously regulates cell migration by regulating actin cytoskeleton organization, and its levels are inversely proportional to neural migration speed. (nature.com)
- In particular, we found that Cul3 controls the abundance of Plastin 3 (Pls3), a novel player of neural cell migration, whose amount is inversely proportional to neural cell migration speed. (nature.com)
- Finally, we found that CRISPR-mediated activation of Cul3 transcription fully rescues neural cell migration defects. (nature.com)
Putative2
- In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
- Our findings were subsequently tested by in vitro experiments, where putative early and later-stage DN1 progenitors from the thymus were purified and their pro- gression into DN2 was measured. (lu.se)
Mechanisms1
- The material from each step isscreened using acellular and in vitro assays for evaluating general toxicity, mechanisms of toxicity, and macrophage function. (cdc.gov)
Immunofluorescence1
- Western blot and immunofluorescence assay (IFA) were used to examine the reactivity of rFg14-3-3e protein to anti- F. gigantica and anti-rFg14-3-3e antibodies, respectively. (biomedcentral.com)
Immunohistochemistry2
- Thymidine Incorporation Assay BrdU offered by a company Thermo Fisher Scientific includes a nucleoside analog that is identified during active DNA synthesis by employing immunohistochemistry. (news-medical.net)
- In addition, immunohistochemistry assay was employed to detect the level of KIF20A, CCNE2, c-Myc, and MMP9 in mice tissues. (bvsalud.org)
Extracellular1
- Traditional assays struggle with evaluating cell phenotypes over time and correlating time-dependent responses with changes in the extracellular environment. (clarkson.edu)
Cell behavior1
- Characterizing collective cell behavior in vitro. (clarkson.edu)
Overexpression1
- PKM2 overexpression promoted cell migration and invasion, and increased SOD2 activity and the intracellular H 2 O 2 level. (oncotarget.com)
Adhesion2
- Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
- Receptor-type protein tyrosine phosphatase alpha (PTPα is a widely expressed transmembrane-bound protein that has been implicated in integrin signaling, focal adhesion formation, and normal cell migration. (ubc.ca)
Macrophages1
- Furthermore, ATP promoted the migration of microglia but not macrophages across the MBEC monolayer. (portlandpress.com)
Compounds1
- The assay can also be used to evaluate therapeutic compounds before clinical use. (ox.ac.uk)
Tissues4
- MSCs derived from placental tissues (PL-MSCs) significantly enhanced EPC migration while BM-MSCs, which are the standard source of MSCs for various clinical applications, did not. (hindawi.com)
- By using protein fractionation and mass spectrometry analysis, we identified several novel candidates for EPC migration enhancing factor in PL-MSCs secretome that could be used to enhance neovascularization in the injured/ischemic tissues. (hindawi.com)
- Vasculogenesis involves migration of EPCs from blood circulation into injured/ischemic tissues where they proliferate and generate new vessels de novo . (hindawi.com)
- In contrast, wounded tissues cultured with growth factors demonstrated a dramatic increase in healing rate as keratinocyte migration completely covered the wounded area by day 6. (mattek.com)
Cancer Research1
- Cell migration and invasion kits are oftenn used in cancer research to study cell motility and invasiveness in response to chemoattractant gradients. (sigmaaldrich.com)
Molecules1
- These proteins control the dynamics of the actin cytoskeleton and thus represent key regulatory molecules that are active during cell migration ( 12 ). (spandidos-publications.com)
Inhibition1
- Kuntz S, Kunz C, Rudloff S. Inhibition of pancreatic cancer cell migration by plasma anthocyanins isolated from healthy volunteers receiving an anthocyanin-rich berry juice. (uni-giessen.de)
Clinical1
- Since 1986, when tests for measuring PSA levels in serum were introduced into clinical practice, early diagnosis and management of prostate cancer has been revolutionized, and much has been learned about the strengths and weaknesses of these assays. (medscape.com)
Methods1
- When applied to in vitro data, our method outperforms standard methods by detecting differences in the migration rates between different cell groups that the other methods could not detect. (ox.ac.uk)
Findings1
- Our findings suggest that miR-637 inhibits glioma invasion and migration and could be a potential diagnostic marker for gliomas. (scirp.org)
Results1
- The results of these experiments demonstrated that PPI increased the susceptibility of HCC to sorafenib while inhibiting SR-HCC cell growth, migration, and invasion. (bvsalud.org)
Formation1
- Or la compréhension des processus intervenant dans la formation de tissus osseux ectopiques dans la cavité buccale - comme les tori - pourrait aider à mettre au point des méthodes moins invasives pour favoriser l'augmentation de la crête alvéolaire et l'amélioration naturelle de la qualité des os en vue de la mise en place d'implants dentaires. (jcda.ca)